| sutterella wadsworthensis gen. nov., sp. nov., bile-resistant microaerophilic campylobacter gracilis-like clinical isolates. | campylobacter gracilis (formerly bacteroides gracilis) is an asaccharolytic, nitrate-positive, urease-negative organism that requires formate and fumarate or hydrogen as a growth additive and may pit agar media. clinical isolates that were obtained primarily from appendiceal and peritoneal fluid specimens and initially were identified in our laboratory as b. gracilis were later found to include "unusual" strains that could be distinguished by biochemical and genetic criteria. these unusual c. gr ... | 1996 | 8573504 |
| in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria. | the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ... | 1996 | 8878617 |
| recently described clinically important anaerobic bacteria: taxonomic aspects and update. | a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ... | 1997 | 9310640 |
| recently described clinically important anaerobic bacteria: medical aspects. | there is still inadequate information on the role of certain newly described or reclassified anaerobes in disease processes, on their normal sites of carriage, and on their antimicrobial susceptibilities. herein, we summarize this information (most of the literature reviewed is from the past 5 years, but a few of the articles are approximately 10 years old). porphyromonas species had seemed to be relatively nonpathogenic, but recent work indicates that this belief is incorrect. p. gingivalis, p ... | 1997 | 9310641 |
| sources and antimicrobial susceptibilities of campylobacter gracilis and sutterella wadsworthensis. | | 1997 | 9310700 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| in vitro activities of mk-826 (l-749,345) against 363 strains of anaerobic bacteria. | the activity of mk-826 was compared to the activities of cefoxitin, ceftriaxone, imipenem, and meropenem against 363 gram-negative and gram-positive anaerobes by using nccls procedures. at least 98% of the strains were susceptible to the carbapenems. all strains of clostridium perfringens, fusobacterium nucleatum, peptostreptococcus, and sutterella wadsworthensis were susceptible to all agents tested. | 2000 | 10898709 |
| antimicrobial susceptibility of non-bacteroides fragilis group anaerobic gram-negative bacilli in europe. | objective: to investigate the in vitro activity of a range of anti-anaerobe antimicrobials against non-bacteroides fragilis group anaerobic gram-negative bacilli isolated in europe. methods: isolates from 15 laboratories in 13 countries were identified by conventional methods. the mics of 20 antibiotics were determined by an agar dilution method. results: there were 488 prevotella spp., 174 fusobacteria, 69 porphyromonas spp., 33 bacteroides spp., 28 bilophila wadsworthia and 16 campylobacter sp ... | 1999 | 11853565 |
| in vitro activities of faropenem against 579 strains of anaerobic bacteria. | the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ... | 2002 | 12384389 |
| sutterella stercoricanis sp. nov., isolated from canine faeces. | morphological, biochemical and molecular genetic studies were carried out on an unknown non-spore-forming, gram-negative, rod-shaped bacterium which was isolated from dog faeces. the bacterium grew under anaerobic conditions, was asaccharolytic, resistant to 20 % (v/v) bile and was oxidase- and urease-negative. phylogenetic analysis based on comparative 16s rrna gene sequencing showed that the unidentified bacterium clustered with sutterella wadsworthensis, although a sequence divergence of >5 % ... | 2004 | 15388713 |
| biochemical differentiation and comparison of desulfovibrio species and other phenotypically similar genera. | seventeen human clinical isolates representing four species of desulfovibrio were characterized using 16s rrna gene sequences and tests for catalase, indole, nitrate, bile, urease, formate-fumarate stimulation, desulfoviridin, motility, and hydrogen sulfide production, plus susceptibility to antimicrobial agents. eighty additional strains representing 10 phenotypically similar genera (bilophila, selenomonas, capnocytophaga, campylobacter, bacteroides, sutterella, anaerobiospirillum, dialister, v ... | 2005 | 16081948 |
| taxonomy--general comments and update on taxonomy of clostridia and anaerobic cocci. | since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. for example, reclassification of the 'bacteroides gracilis group' revealed that sutterella wadsworthensis is actually responsible for the antimicrobial resistance seen in that group. with clostridia and anaerobic cocci, as with other taxa, there have ... | 2002 | 16887670 |
| comparison of the cecal microbiota of domestic and wild turkeys. | the extent to which production methods alter intestinal microbial communities of livestock is currently unknown. as the intestinal microbiota may affect animal health, nutrition, and food safety, a baseline comparison of the cecal communities of domestic and wild turkeys was performed. oligonucleotide fingerprinting of ribosomal rna (rrna) genes (ofrg) of 2,990 16s rrna clones and dot blot quantification of dominant populations were used to identify the dominant bacterial taxa. seventy-three per ... | 2008 | 18183454 |
| sutterella parvirubra sp. nov. and megamonas funiformis sp. nov., isolated from human faeces. | three strains of anaerobic, non-spore-forming, gram-negative coccobacilli (yit 11816t, yit 11817 and yit 11818) were isolated from human faeces. on the basis of 16s rrna gene sequence similarity, these strains were shown to belong to the family alcaligenaceae and to be related to the type strain of sutterella stercoricanis (94.9 %) and to sutterella wadsworthensis wal 7877 (94.3 %); the similarity to strains of any other species with a validly published name within the family alcaligenaceae was ... | 2008 | 18398204 |
| activity of a novel carbapenem, doripenem, against anaerobic pathogens. | doripenem, a synthetic 1-beta-methyl carbapenem, has a broad-spectrum of activity against almost all species of anaerobic bacteria, including all bacteroides fragilis group species, most with mic(90) results at <or=1 microg/ml (with the exception of sutterella wadsworthensis). against clostridium difficile strains, it has a narrow range of inhibitory concentrations (1-4 microg/ml) that "may be achievable in the colon", potentially resulting in lower disease rates. it has been shown to be active ... | 2009 | 19249176 |
| parasutterella excrementihominis gen. nov., sp. nov., a member of the family alcaligenaceae isolated from human faeces. | a novel, strictly anaerobic, non-spore-forming, gram-negative coccobacillus (strain yit 11859(t)) was isolated from human faeces. biochemically, this strain was largely unreactive and was asaccharolytic. growth of strain yit 11859(t) in peptone-yeast extract broth produced no visible turbidity, and a trace amount of propionate was detected as an end product of metabolism. 16s rrna gene sequence analysis showed that strain yit 11859(t) was related most closely to the type strains of sutterella sp ... | 2009 | 19542131 |
| molecular inventory of faecal microflora in patients with crohn's disease. | intestinal microbial community is involved in the pathogenesis of crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. using sequence analysis of randomly cloned bacterial 16s ribosomal dna, the dominant faecal species from four crohn's disease patients and four controls were compared. whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly re ... | 2004 | 19712374 |
| parasutterella secunda sp. nov., isolated from human faeces and proposal of sutterellaceae fam. nov. in the order burkholderiales. | a novel, strictly anaerobic, non-spore-forming, gram-reaction-negative coccobacillus bacterium, designated strain yit 12071(t), was isolated from human faeces. biochemically, this strain was largely unreactive and asaccharolytic. growth of this strain in peptone-yeast-extract broth was weak, producing no visible turbidity, and no short-chain fatty acids were detected as an end product of metabolism. following 16s rrna gene sequence analysis, strain yit 12071(t) was found to be most closely relat ... | 2011 | 20400667 |
| a comprehensive evaluation of colonic mucosal isolates of sutterella wadsworthensis from inflammatory bowel disease. | inflammatory bowel disease (ibd) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. twenty-six clinical isolates of sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had ibd. s. wadsworthensis was initially more frequently isolated from ibd subjects, hence this comprehensive study was undertaken to elucidate its ro ... | 2011 | 22073125 |
| application of novel pcr-based methods for detection, quantitation, and phylogenetic characterization of sutterella species in intestinal biopsy samples from children with autism and gastrointestinal disturbances. | abstract gastrointestinal disturbances are commonly reported in children with autism and may be associated with compositional changes in intestinal bacteria. in a previous report, we surveyed intestinal microbiota in ileal and cecal biopsy samples from children with autism and gastrointestinal dysfunction (aut-gi) and children with only gastrointestinal dysfunction (control-gi). our results demonstrated the presence of members of the family alcaligenaceae in some aut-gi children, while no contro ... | 2012 | 22233678 |