| a new procedure for efficient recovery of dna, rna, and proteins from listeria cells by rapid lysis with a recombinant bacteriophage endolysin. | a method for the rapid lysis of listeria cells, employing a recombinant listeria bacteriophage a118 lytic enzyme (ply118), is described. the procedure can be used with all listerial species. it enables fast, efficient, and gentle recovery of dna, rna, or native cellular proteins from small-scale (2- to 5-ml) cultures. moreover, this approach should be very useful in analytical detection and differentiation of listeria strains when the release of native nucleic acids or proteins is required. | 1995 | 7540820 |
| bacteriophage receptors on listeria monocytogenes cells are the n-acetylglucosamine and rhamnose substituents of teichoic acids or the peptidoglycan itself. | different approaches were used to examine the function of teichoic acids (ta) as phage receptors among selected listeria strains, and to identify and characterize specific receptor structures of host cells belonging to different serovars. this included successive removal of cell wall constituents, preparation and purification of ta, and glc analysis of ta components. adsorption of listeria monocytogenes bacteriophages could be inhibited by polyvalent antisera, specific lectins and addition of pu ... | 1996 | 8936325 |
| transposon-induced mutations in two loci of listeria monocytogenes serotype 1/2a result in phage resistance and lack of n-acetylglucosamine in the teichoic acid of the cell wall. | teichoic acid-associated n-acetylglucosamine and rhamnose have been shown to serve as phage receptors in listeria monocytogenes serotype 1/2a. we generated and characterized two single-copy tn916deltae mutants which were resistant to phage a118 and several other serotype 1/2a-specific phages. in one mutant the insertion was immediately upstream of the recently identified ptshi locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other ... | 1999 | 10543788 |
| complete nucleotide sequence, molecular analysis and genome structure of bacteriophage a118 of listeria monocytogenes: implications for phage evolution. | a118 is a temperate phage isolated from listeria monocytogenes. in this study, we report the entire nucleotide sequence and structural analysis of its 40 834 bp dna. electron microscopic and enzymatic analyses revealed that the a118 genome is a linear, circularly permuted, terminally redundant collection of double-stranded dna molecules. no evidence for cohesive ends or for a terminase recognition (pac) site could be obtained, suggesting that a118 viral dna is packaged via a headful mechanism. p ... | 2000 | 10652093 |
| comparative genomics of the late gene cluster from lactobacillus phages. | three prophage sequences were identified in the lactobacillus johnsoni strain ncc533. prophage lj965 predicted a gene map very similar to those of pac-site streptococcus thermophilus phages over its dna packaging and head and tail morphogenesis modules. sequence similarity linked the putative dna packaging and head morphogenesis genes at the protein level. prophage lj965/s. thermophilus phage sfi11/lactococcus lactis phage tp901-1 on one hand and lactobacillus delbrueckii phage ll-h/lactobacillu ... | 2000 | 10998330 |
| cloning and characterization of m.lmoa118i, a novel dna:m4c methyltransferase from the listeria monocytogenes phage a118, a close homolog of m.ngomxv. | a homolog of m.ngomxv dna:m4c methyltransferase has been identified among the open reading frames deduced from the genomic sequence of listeria monocytogenes phage a118 [loessner et al., 2000]. the gene coding for this putative protein has been cloned in escherichia coli and its enzymatic activity in vivo in this host have been analyzed. remarkably, despite m.ngomxv and m.lmoa118i exhibit high sequence similarity (58% identical and 19% conservatively substituted residues), their target preferenc ... | 2001 | 11720310 |
| genome analysis of an inducible prophage and prophage remnants integrated in the streptococcus pyogenes strain sf370. | the mitomycin c inducible prophage sf370.1 from the highly pathogenic m1 serotype streptococcus pyogenes isolate sf370 showed a 41-kb-long genome whose genetic organization resembled that of sf11-like pac-site siphoviridae. its closest relative was prophage nih1.1 from an m3 serotype s. pyogenes strain, followed by s. pneumoniae phage mm1 and lactobacillus phage phig1e, listeria phage a118, and bacillus phage spp1 in a gradient of relatedness. sequence similarity with the previously described pr ... | 2002 | 12441069 |
| functional regulation of the listeria monocytogenes bacteriophage a118 holin by an intragenic inhibitor lacking the first transmembrane domain. | we have dissected the functional properties of the holin encoded by listeria monocytogenes bacteriophage a118. native hol118 was cloned into lambdadeltasthf, devoid of the s holin, and tested in an e. coli background. surprisingly, it caused very late cell lysis, beginning at 80 min after induction. immunological analyses demonstrated that hol118 appears in the cytoplasmic membrane shortly after infection. the hol118 gene features a dual start motif similar to lambda s. therefore, different n-te ... | 2003 | 12657053 |
| genome and proteome of listeria monocytogenes phage psa: an unusual case for programmed + 1 translational frameshifting in structural protein synthesis. | psa is a temperate phage isolated from listeria monocytogenes strain scott a. we report its complete nucleotide sequence, which consists of a linear 37 618 bp dna featuring invariable, 3'-protruding single stranded (cohesive) ends of 10 nucleotides. the physical characteristics were confirmed by partial denaturation mapping and electron microscopy of dna molecules. fifty-seven open reading frames were identified on the psa genome, which are apparently organized into three major transcriptional u ... | 2003 | 14507382 |