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fungal nucleic acids as interferon inducers.nucleic acids isolated from the fungi aspergillus niger x11, piptoporus betulinus and ganoderma applanatum reduced the number of vaccinia virus plaques in chick embryo fibroblast (cef) tissue culture and when administered intravenously to white mice protected them against lethal infection with tick borne encephalitis virus strain k5 (tbe). in cef tissue culture the nucleic acids of the studied fungi were found to induce small but detectable amounts of a substance with the character of interferon ...197994746
studies of rna isolated from piptoporus betulinus as interferon inducer.crude rna isolated from piptoporus betulinus and two fractions of it obtained after the chromatography contain single-stranded rna. the crude rna, but not its chromatographic fractions, are resistant to the pancreatic rna-ase. the crude preparation of rna from p. betulinus contains beside ribose, considerable amounts of glucose, galactose and mannose. the crude rna from p. betulinus induces in the human fibroblasts interferon with specific activity of 400 units per mg of protein.1978219808
4-methylmorpholine n-oxide-methyl sulfoxide soluble glucan of piptoporus betulinus. 19902081343
[increased levels of cesium-137 and cesium-134 in 34 mushroom species following the reactor accident at chernobyl].from september to december of 1986, 85 samples of 34 species of wild mushrooms were collected, mostly in northern baden and occasionally in neighboring areas. cs-137, cs-134, and the natural nuclide k-40 were determined by gamma-spectrometry. samples from the rhine valley and the kraichgau were generally much less contaminated than those from the black forest. with one exception, their radiocesium levels were below the common market limit of 600 bq/kg. the exception was a sample of laccaria amet ...19873660949
ether extracts from the fruiting body of piptoporus betulinus as interference inducers. 19744838508
the influence of inhibitors of cellular synthesis and uv irradiation on interferon induction by rna from piptoporus betulinus.the effect of cycloheximide, chloramphenicol, ethidium bromide (eb), aurin tricarboxylic acid (ata), and actinomycin d on the production of interferon (ifn) in human embryo fibroblasts (hat) and l929 cells, stimulated with rna from piptoporus betulinus (pb-rna) was studied. treatment of hat cells with cycloheximide superinduced the production of ifn. subsequent treatment with cycloheximide and actinomycin d chloramphenicol and actinomycin d or ata and actinomycin d early after induction also enh ...19827149923
cellulase activity of higher fungi.the method of liquefaction of gel from cross-linked cellulose was used for monitoring the cellulolytic activity of 114 cultures of higher fungi, 47 of which belonged to pleurotus ostreatus. all cultures of p. ostreatus had a low activity. the highest cellulase activity, manifested by piptoporus betulinus, was comparable with that of tricloderma viride qm6a.19807191386
volatile constituents of wood-rotting basidiomycetes.phytochemical investigation of the hydrodistillation products of the basidiomycetes fomitopsis pinicola, piptoporus betulinus, gloeophyllum odoratum and trametes suaveolens led to the identification of numerous mono- and sesquiterpenes as well as many aliphatic alcohols, aldehydes and ketones and some aromatic compounds. in addition, some diterpenes were identified as constituents of fomitopsis pinicola. the absolute configuration of some terpenes was determined200011014259
piptamine, a new antibiotic produced by piptoporus betulinus lu 9-1. 200011099232
novel hydroquinone as a matrix metallo-proteinase inhibitor from the mushroom, piptoporus betulinus.the novel hydroquinone, (e)-2-(4-hydroxy-3-methyl-2-butenyl)-hydroquinone, and known compound, polyporenic acid c, were isolated as matrix metallo-proteinase inhibitors from the mushroom, piptoporus betulinus.200212596882
anti-inflammatory lanostane-type triterpene acids from piptoporus betulinus.six lanostane-type triterpene acids were isolated from the fruiting bodies of piptoporus betulinus. they were identified as polyporenic acids a (1) and c (2), three derivatives of polyporenic acid a (3-5), and a novel compound, (+)-12 alpha,28-dihydroxy-3 alpha-(3'-hydroxy-3'-methylglutaryloxy)-24-methyllanosta-8,24(31)-dien-26-oic acid (6). all these compounds suppressed the 12-o-tetradecanoylphorbol-13-acetate (tpa)-induced edema on mouse ears by 49-86% with a 400 nmol/ear application.200312932134
anti-inflammatory and anti-hyaluronate lyase activities of lanostanoids from piptoporus betulinus. 200415712671
estimation of bound and free fractions of lignocellulose-degrading enzymes of wood-rotting fungi pleurotus ostreatus, trametes versicolor and piptoporus betulinus.liquid cultures with cellulose and solid state fermentation cultures on wheat straw of the white-rot fungi pleurotus ostreatus and trametes versicolor and the brown-rot fungus piptoporus betulinus were assayed for the free and solid fraction-bound activity of lignocellulose-degrading enzymes. the majority of the ligninolytic enzymes laccase and mn peroxidase was detected in the free fraction of p. ostreatus and t. versicolor. the endocleaving enzymes endo-1,4-beta-glucanase, endo-1,4-beta-mannan ...200616125911
use of differential scanning calorimetry for structural analysis of fungally degraded wood.this paper assesses the potential use of differential scanning calorimetry for analyzing sound and decayed wood. with sound wood, this method permitted the detection of cellulose, hemicellulose, and lignin components as discrete peaks of combustion at defined temperatures. characteristic changes in the calorimetric thermogram of birchwood (temperature of maxima, peak height, and peak area) were obtained from wood samples degraded by the basidiomycetes fomes fomentarius and piptoporus betulinus. ...198616347209
evaluation of the monophyly of fomitopsis using parsimony and mcmc methods.to evaluate the monophyly of fomitopsis and elucidate phylogenetic relationships of its members, partial nuclear large subunit (partial 28s) ribosomal rna genes were sequenced from 10 species of fomitopsis and 15 related species. phylogenetic analyses indicated that fomitopsis was phylogenetically heterogeneous and its members were divided into three subgroups. the constrained tree excluding f. palustris (the type species of pilatoporus) from fomitopsis core group was rejected, thus rejecting th ...200516457351
degradation of cellulose and hemicelluloses by the brown rot fungus piptoporus betulinus--production of extracellular enzymes and characterization of the major cellulases.piptoporus betulinus is a common wood-rotting fungus parasitic for birch (betula species). it is able to cause fast mass loss of birch wood or other lignocellulose substrates. when grown on wheat straw, p. betulinus caused 65% loss of dry mass within 98 days, and it produced endo-1,4-beta-glucanase (eg), endo-1,4-beta-xylanase, endo-1,4-beta-mannanase, 1,4-beta-glucosidase (bg), 1,4-beta-xylosidase, 1,4-beta-mannosidase and cellobiohydrolase activities. the fungus was not able to efficiently deg ...200617159214
chemical constituents of stereum subtomentosum and two other birch-associated basidiomycetes: an interspecies comparative study.metabolites of the wood-rotting fungus stereum subtomentosum pouzar (basidiomycetes, order russulales, family stereaceae) occurring on birch (betula pendula roth) trees were phytochemically investigated for the first time. three main metabolite chemotypes present in meoh extracts of the fruit bodies, viz. steroids, fatty acids, and water-soluble sugars, were fractionated, isolated, and identified by 1d/2d nmr-spectroscopic analyses, nmr data comparisons, and chemical correlations combined with g ...200818493960
autofluorescence of the fruiting body of the fungus macrolepiota rhacodes.the autofluorescence (primary fluorescence, af) of the freshly collected fruiting bodies of the fungus macrolepiota rhacodes was studied in a zeiss jenalumar fluorescence microscope at a blue and a green excitation. the strongest yellow af at blue excitation was displayed by irregular granules on the surface of the fungal pileus. a weaker yellow-green af was exhibited by spherical cells and hyphae in the central part of the pileus while basidiospores emitted somewhat stronger af. at green excita ...200919381481
intraspecific variability in growth response to cadmium of the wood-rotting fungus piptoporus betulinus.the intraspecific variability in growth response to cadmium (cd) on agar media and in liquid culture was studied among fourteen strains of a wood-rotting fungus piptoporus betulinus. the variability of cd tolerance was found to be very high. the ed(50) ranged from 6.8 μm cd in the most sensitive strain, up to 255.1 μm in the most resistant one. on agar media the addition of cd to nutrient media resulted in reduction of relative growth rate and increased lag time. while the reduction of growth ra ...200221156514
α-(1 → 3)-d-glucans from fruiting bodies of selected macromycetes fungi and the biological activity of their carboxymethylated products.purpose of work: to show biological activity of carboxymethylated α-(1 → 3)-d-glucans isolated from the selected macromycetes fungi on human tumor and normal cells. water-insoluble, alkali-soluble polysaccharides (wip) were isolated from fruiting bodies of four macromycetes fungi: lentinus edodes, pleurotus ostreatus, piptoporus betulinus and laetiporus sulphureus. the structure of the polysaccharides was determined using composition analysis, methylation analysis, fourier transform infrared spe ...201021188616
enhancement of autofluorescence of the brown-rot fungus piptoporus betulinus by metal ions.the autofluorescence (primary fluorescence, af) of agar cultures of the brown-rot fungus piptoporus betulinus was investigated in zeiss jenalumar and nikon eclipse 8201 fluorescence microscopes at various excitations. the strongest af of hyphae was found in minimal medium with glucose, where the hyphae exhibited green af at violet (450 nm) excitation and red af at green (570 nm) excitation. addition of metals to cultivation media led to enhanced white-blue af in the presence of co (at 450 nm) an ...201021253910
investigation of antiproliferative effect of ether and ethanol extracts of birch polypore medicinal mushroom, piptoporus betulinus (bull.:fr.) p. karst. (higher basidiomycetes) in vitro grown mycelium.this paper describes the study conducted to evaluate the antiproliferative activity of ether and ethanol extracts isolated from piptoporus betulinus against cancer-derived cells. the fungal material used for extract preparation and further experiments was obtained from in vitro grown strains of p. betulinus. to the best of the authors’ knowledge, this is the first study evaluating antiproliferative potential of in vitro cultured birch polypore fungus. the effect of ether and ethanol extracts on ...201122181840
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