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[study on the pathogenecity of chrysosporium species (author's transl)]. 197994426
case for diagnosis. 197896370
the prevalence of adiaspiromycosis in three sympatric species of ground squirrels.lungs from three species of ground squirrels collected in south central saskatchewan were examined by histopathology and a digestion technique for adiaspores of emmonsia crescens. two of 81 (2.5%) citellus richardsoni, 3 of 17 (17.6%) c. tridecemlineatus and 35 of 44 (79.5%) c. franklini were infected. infection was more common in adults than in young-of-the-year. tissue digestion was the more sensitive method for detecting adiaspores. possible reasons for the difference in prevalence among the ...1978567700
adiaspiromycosis in czechoslovakian mammals.the examination of 46 mammalian species in czechoslovakia revealed adiaspiromycosis caused by the emmonsia crescens in 22 species of free living animals; e. parva was the etiological agent in 3 species. the disease was most frequently found in carnivores of the family mustelidae.1977564081
[current state of immunology of visceral mycoses]. 1978366947
occurrence of histoplasma capsulatum darling, 1906 in israel, with a review of the current status of histoplasmosis in the middle east.the isolation of histoplasma capsulatum from a bat cave in the galilee region of israel is reported along with the discovery of a bat (myotis myotis) infected by h. capsulatum. soils collected in avian and chiropteran habitats throughout israel also yielded the following fungi of medical interest: arthroderma quadrifidum, a. tuberculatum, candida parapsilosis, chrysosporium indicum, c. keratinophilum, c. tropicum, cryptococcus neoformans, ctenomyces serratus, microsporum gypseum, and petriellidi ...1977842775
adiaspiromycosis.we have then a disease that, for many years (since 1942), was known only to infect lower animals; after a sporadic encapsulated adiaspore was observed by doby-dubois in 1964 in a patient's lung, a widely disseminated, clinically symptomatic case was reported from czechoslovakia, rapidly followed by three mildly disseminated cases, one from russia and two from guatemala, with innumerable granulomas similar to the patient of kodousek et al. patient. alert observers contributed descriptions of sing ...1978364386
extracellular enzyme system utilized by the fungus sporotrichum pulverulentum (chrysosporium lignorum) for the breakdown of cellulose. 3. purification and physico-chemical characterization of an exo-1,4-beta-glucanase.an exo-1,4-beta-glucanase from culture solution of the rot fungus sporotrichum pulverulentum (formerly called chrysosporium lignorum) grown on powder cellulose as the sole carbon source has been extensively purified and characterized with respect to some physico-chemical properties. the purification has been carried out in a five-step procedure comprising chromatography on deae-sephadex, gel filtration on polyacrylamide p-150, activation on a dowex 2-x8 anion exchanger, chromatography on concana ...1975235428
extracellular enzyme system utilized by the fungus sporotrichum pulverulentum (chrysosporium lignorum) for the breakdown of cellulose. 1. separation, purification and physico-chemical characterization of five endo-1,4-beta-glucanases.five endo-1,4-beta-glucanases (ec 3.2.1.4) have been separated from culture solutions of the rot fungus sporotrichum pulverulentum (formerly called chrysosporium lignorum) grown on powder cellulose as the sole carbon source. they have been extensively purified and characterized with regard to some physicochemical properties. the purifications have been carried out on a quantitative basis, the purity of the enzymes being tested in several ways. after purification they all showed one single protei ...1975235427
specific immunological test for the rapid identification of members of the genus histoplasma.a sensitive and specific immunological method was developed for rapid identification of the mycelial forms of histoplasma capsulatum var. capsulatum, h. capsulatum var. duboisii, and h. farciminosum and for separation of these pathogenic fungi from morphologically similar hyphomycetes and other fungal pathogens. this method is based on the fact that all of the histoplasma spp. produce h and m histoplasmin antigens, whereas the other fungi do not. inocula consisting of heavy mycelial growth from ...197656339
induction of colonial growth and replica plating of the white rot basidiomycete phanaerochaete chrysosporium.a medium containing l-sorbose and sodium deoxycholate was devised which induces colonial growth of phanaerochaete chrysosporium in the presence of a variety of supplements. the restricted size and heavy conidial production of the colonies permits high plating densities and the use of a replica-plating technique for copying the pattern of colony growth.197828081
chrysosporium species as fungal air pollutants.air filters utilized in monitoring industrial air pollutants were analyzed for the presence of airborne fungi. the genera of fungi isolated were essentially those isolated in other areas of the united states; however, chrysosporium species, unreported in previous airborne surveys, were found in significant numbers. thirty-six genera of fungi were recovered over a one-year period. aspergillus and chrysosporium were isolated most frequently from the filter samples examined.1979760619
ligninolytic enzyme system of phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation.the relationship between growth, nutrient nitrogen assimilation, and the appearance of ligninolytic activity was examined in stationary batch cultures of the wood-destroying hymenomycete phanerochaete chrysosporium burds. grown under conditions optimized for lignin metabolism. a reproducible sequence of events followed inoculation: 0 to 24 h, germination, linear growth, and depletion of nutrient nitrogen; 24 to 48 h, cessation of linear growth and derepression of ammonium permease activity (demo ...1978690075
effects of temperature and fungicides on chrysosporium pannorum (link) hughes.c. pannorum was isolated in low frequencies and only in the colder months from untreated soil and leaves and from those treated with captan, dicloran and thiram. the fungus was isolated throughout the year as the major species from verdasan-treated substrates and it occurred with increased frequencies immediately after application of the fungicide. the abundance of c. pannorum on verdasan-treated substrates was attributed to the ability of the fungus to utilise the fungicide rather than to the a ...1979575973
adiaspiromycosis of human skin caused by emmonsia crescens.two cases of cutaneous adiaspiromycosis by emmonsia crescens are reported. this is the first human skin infection by this species and is the first report of its kind in man from india. in the first patient, the agent was demonstrated in koh mounts, histology and culture from irregular, pigmented skin plaques on the right gluteal area. the lesion also contained calcium. in the second patient the fungus was demonstrated histologically in a knee lesion. the agent had elicited a histiocytic and gian ...1979575679
isolation of keratomycetes from the soil of wild animal cages and enclosures in the zoo of the parco nazionale d'abruzzo, italy.the keratinophilic fungi present in the soil of wild animal cages and enclosures in the zoo of the "parco nazionale d'abruzzo", at pescasseroli, italy were studied. the goal of the investigation was to determine what species of such fungi existed in wild animal habitats in that area and what variation there may be in their frequency in connection with the seasons. the most prevalent fungus was trichophyton ajelloi, followed in decreasing order by the chrysosporium species (c. keratinophilum, c. ...1978572094
antigenic activity of emmonsia crescens mutants.the antigenic properties of 3 uv mutants of emmonsia crescens emmons et jellison 1960 were compared with the original wild strain, further with other 13 e. crescens strains, with e. parva, e. brasiliensis, e. ciferrina and chrysosporium pannorum. for this purpose the strains were used for the immunisation of 70 rabbits. the formation of specific igm-and igg-antibodies was examined in their blood by means of several serologic reactions, and the capability of sensitizing the organism was proved by ...1978571532
spontaneous infection of white laboratory mice with emmonsia crescens emmons et jellison, 1960 under natural conditions.fifty white laboratory mice were planted in a microbiotope in which adiaspiromycosis has been detected in 52 microtus arvalis during the last 10 years. four of the white mice became infected with adiaspiromycosis. serological tests revealed the infection in two mice exposed for 3 months. after 4 months' exposure, another two mice were found to be infected and the positive serological results were confirmed by microscopical examination which revealed adiaspores in their lungs. the infection was t ...1978570537
adiaspiromycosis in the lungs of a goat. 1978566741
the effects of temperature on growth of four high arctic soil fungi in a three-phase system.the effect of temperature on the growth of chrysosporium pannorum, cylindrocarpon sp., penicillium janthinellum, and phoma herbarum, isolated from tundra soils, was studied. the growth in two systems, glucose-mineral agar plates and sand, moistened with glucose-mineral broth, was compared. all isolates showed an exponential increase in mass (measured as protein increase) in sand and a linear rate of extension on agar. radial increase on agar was shown not to be a good index of growth in sand. tr ...1978565246
[budding of the parasitic forms of the fungus emmonsia crescens emmons et yellison, 1960]. 1977564442
[disseminated pulmonary adiasporomycosis. (report of one case) (author's transl)]. 1977564165
[tissue morphology of the causative agents of visceral mycoses. studies of polysaccharide and protein histochemistry. (6. rhinosporidium seeberi and emmonsia crescens)]. 19751095926
[mycological control of laboratory animals]. 1975821323
experimental infection of white mouse with chrysosporium and paecilomyces.spf white mice were inoculated intraperitoneally with 5 strains of saprophytic fungi of the mycelial genera chrysosporium (c. keratinophilum, c. tropicum) and paecilomyces (p. lilacinus, p. marquandii, p. victoriae). the fungi caused granulomatous lesions in the peritoneal cavity and they were recultured (except p. lilacinus and p. marquandii) two months after inoculation. spores, short hyphae and budding cells of all the fungi were observed in the granulomas stained by periodic-acid schiff (pas ...1977563979
[different behavior of 22 strains of emmonsia cifferi and montemartini 1959, a monilial fungus, in the lungs of laboratory mice in comparison with their parasitic morphology in vitro].the 22 strains of emmonsia ciferri & montemartini 1959, inoculated intranasally to laboratory mice are not equally virulent. one month after the inoculation, 15 of the strains had produced adiaspores 120 t9 190 micrometer in diameter in the lung. another strain produced adiaspores measuring 44 micrometer and 2 others measuring 20 micrometer or 10 micrometer. the remaining 4 strains did not develop in the lung tissue. four thermophilic strains, which in vitro have adiaspores measuring 8 to 15 mic ...1977562477
avirulence and decreased virulence in mutants of emmonsia crescens, a causative agent of adiaspiromycosis.three mutants of emmonsia crescens with altered morphology and pathogenicity for mice are described. the mutant m-1 is avirulent and does not form adiaspores of normal morphology on agar medium at 37 degree c. the mutants m-2 and m-12 have a considerably decreased virulence. the pathogenicity of e. crescens depends on the ability to differentiate adiaspores of perfect morphology. the loss of virulence and the decrease of virulence of mutants seem to be caused by the pleiotrophic effect of mutati ...1977560338
the atmospheric fungal flora of the athens metropolitan area.in the research programme of the department of microbiology of the athens university the nature of the mycological flora of the athenian air was studied. the research took place during the calendar year 1971. the open air was sampled twice weekly from two observation stations. the open plate technique was used, petri dishes containing sabourand's agar being exposed for 15 minutes. a total of 180 plates were exposed, and 1714 fungal colonies were isolated; these were subcultured and identified as ...19751207717
importance of free living mustelid carnivores in circulation of adiaspiromycosis.the study of adiaspiromycosis in 8 species of free living mustelid carnivores (266 specimens) revealed the average intensity of infection to be 41.4%. the highest incidence rate was found in the exoanthropic species putorius eversmanni (73.1%) and martes martes (72.2%) while the lowest was observed in the hemisynanthropic species putorius putorius (30.6%). the stone marten (martes foina)) is a new, still unknown reservoir host of c. parvum var. crescens, c. parvum for which putorius eversmanni a ...1977559252
[research of keratinophiles in the soil and their effects on human cutaneous pathology in the lyons area].between 1967 and 1977, the systematic research of keratinophiles of the ground in a radius of 30--50 km around lyon, has shown the ubiquitarian predominance of microsporum nanum, which is probably the most ancient occupant of the ground. the most intensive areas of human and animal occupation in the rhodanian tract show the predominance of microsporum gypseum, which is scarcely found in man. sporadic localizations, tightly limites, bound to cirulcation axes and international gathering, let us de ...1979488487
[dermatophytosis, pseudo-dermatophytosis and healthy cutaneous portage observed in platyrhini and cynomorpha monkeys (author's transl)].the most frequent tinea of the subhuman primates are microsporosis due to microsporum canis or trichophytosis at trichophyton mentagrophytes and t. simii. the t. mentagrophytes, an anthropo-zoophilic species must be differenciated from t. rubrum and t. interdigitale, dermatophytes of the man; due to the importance of that diagnosis in primate mycology, some tests are used for the differenciation. between the 226 monkeys investigated it was registered: 2 trichophytosis at t. mentagrophytes (an on ...1977418728
[comparative sensitivity of several laboratory animals to infection by nasal instillation of the saprophytic phase of emmonsia crescens emmons & jellison, 1960: frequency and intensity of parasitism, histological reactions].comparative observations were made on the development of emmonsia crescens in the lungs of laboratory rats and mice, golden hamsters and guinea pigs after a nasal instillation of a heavy suspension of the saprophytic phase of the fungus. 95% of 80 experimental rats were found to be parasited against 80% of 200 inoculated mice, while only 30% of 70 hamsters and all of 4 guinea pigs showed an infection. the lungs of the mice, rats and guinea pigs were frequently more heavily infected than those of ...19751239660
keloidal blastomycosis (lobo's disease) with lymphatic involvement: a case report.keloidal blastomycosis (lobo's disease) is reported in a 34-year-old man from the brazilian amazon area. his left ear and posterior auricular region were involved. he is the eighth of 99 patients known to have lymph node involvement.1976942708
[in vitro formation of adiospores in 10 strains of emmonsia cresens, the fungal agent of adiospiromycosis].emmonsia crescens emmons and jellison 1960 is a dimorphic fungus responsible for pulmonary mycoses in animals and man. temperature was the essential factor influencing the development of the parasitic phase : the adiaspores, which formed better at 37 degrees than at 40 degrees. certain media (medium with blood, with egg, sabouraud...) also favoured the growth of adiaspores. the amount of co2 in the ambiant atmosphere had no effect on the formation of adiaspore phase. on the other hand, the liqui ...1975129238
[contribution to the antigenic activity within the genus chrysosporium corda]. 1976986323
[scanning electron microscopy of adiaspiromycosis agent]. 1976986547
mycotic flora in the lower digestive tract of feral pigeons (columba livia) in the el paso, texas area.fourteen species of fungus were isolated from the lower digestive tract of 39 of 80 pigeons. sixteen pigeons had concurrent isolations while two harbored three species. fungi isolated were allescheria boydii, aspergillus spp., candida krusei, chrysosporium spp., geotrichum candidum, mucor spp., paeciliomyces spp., penicillium spp., rhizopus spp., rhodotorula spp., scopulariopsis spp., streptomyces spp., and trichosporon cutaneum. there was no apparent evidence that these fungi were associated wi ...19761255918
mutants of emmonsia crescens--their pathogenicity and size of adiaspores in vivo.the pathogenicity of seven morphological mutants of emmonsia crescens was tested by means of intraperitoneal inoculation in mice. all mutants caused adiaspiromycosis. adiaspores were isolated from granulomas after 2 months and their diameters were determined. adiaspores from granulomas caused by five mutants (m-5, m-6, m-8, m-9 and m-16) were significantly smaller than adiaspores from granulomas caused by the wild strain, from which the mutants were derived. two mutants (m-6 and m-9) produced ad ...1976987978
adiaspiromycosis in the lungs of a dog. 19761033930
[antigenic characteristics of emmonsia crescens emmons et jellison 1960]. 19761034207
interspecific affinity among keratinolytic fungi associated with birds.twelve species of keratin decomposers isolated from 923 samples of feathers, nests and pellets of 90 species of free-living birds were clustered in 4 "econs" (numerical ecological groups): (a) artroderma tuberculatum, a. ciferrii, a. multifidum, a. cuniculi (associated with the birds nesting in hollows); (b) ctenomyces serratus, arthroderma quadrifidum, chrysosporium evolceanui (moderately alkalophilic species associated with the birds having a frequent contact with the soil); (c) chrysosporium ...19761034590
histopathological changes and the genesis of adiaspiromycomas in mice infected intraperitoneally with emmonsia cresens emmons et jellison, 1960.following an intraperitoneal inoculation with a suspension of aleuries of the funtus emmonsia crescens, characteristic adiaspiromycomas originate in the abdominal cavity; a generalisation of the infection was caused by lymphogenic and haematogenic dissemination. at first, fragments of the mycelium became implanted and started to grow on the peritoneum; these were quickly destroyed by a gradually increasing leukocytic reaction. in the first month of infection nodules were formed mainly on predisp ...19761035183
[emmonsia crescencse emmons et jellison, 1960- a common cause of adiaspiromycosis in squirrels (sciurus vulgaris l.) in czechoslovakia]. 19761035789
[10th case of lobomycosis observed in french guiana].lobomycosis a disease specific to the south-american continent; it is rare but not exceptional, since 11 cases have already been observed in french guyana. a propos of the 10th case, the authors recall the circumstances of the discovery and the basic elements of the microscopical diagnosis. the actual progress in the in vivo culture techniques should allow a better knowledge of the pathogen agent in the future.19761037625
isolation of keratinophilic fungi from soil in pavia, italy.the examination of keratinophilic fungi in 125 soil samples collected in pavia, italy revealed the following organism: microsporum gypseum 23, m. vanbreuseghemii 4, arthroderma uncinatum 4, ctenomyces serratus 5, chrysosporium tropicum 5, unidentified gymnoascacean 1. arthroderma uncinatum, m. gypseum, m. vanbreuseghemii were isolated from a city park and cultivated soils; c. tropicum, ct. serratus and an unidentified gymnoascacean were found only in soils from natural woods. the distribution of ...19751091998
extracellular enzyme system utilized by the fungus sporotrichum pulverulentum (chrysosporium lignorum) for the breakdown of cellulose. 2. activities of the five endo-1,4-beta-glucanases towards carboxymethylcellulose.a complementary discussion is given of an earlier presented theory for viscometric determination of enzymic activity towards polymer substrates. five different, purified endo-1,4-beta-glucanases from the fungus sporotrichum pulverulentum have been characterised with respect to their molecular activities(am) and michaelis-menten constants (km). the errors in the am and km values are discussed. the am value seems to be more valuable as a distinguishing parameter than the km value.19751122913
classification of some gymnoascaceae by pyrolysis-gas-liquid chromatography using added marker compounds.methyl esters of fatty acids were added to samples before pyrolysis to superimpose peaks of known retention time on the pyrochromatograms. these peaks were useful in calibrating the retention time scales so that peaks on different chromatograms could be appropriately homologized. the study included 3 species of nannizzia, 3 strains of trichophyton rubrum and 1 strain each of gymnoascus uncinatus, g. reesii, arachniotus hyalinosporus, chrysosporium pannorum and gliocladium viride. pyrograms of th ...19751124463
a study of the kinetics of microsporogenesis in campelia zanonia (l.) h.b.k.during maturation, microspores pass through a series of morphologically distinguishable stages or compartments. a study has been made of the systematic fluctuations in the frequency of microspores in these compartments, when plants are grown under rigidly controlled conditions. a new approach to the construction of cumulative flux rate curves is described; these give the number of cells passing the compartment boundaries per unit time. the curves obtained indicate that simple models, which assum ...19751170019
isolation of emmonsia crescens emmons et jellison 1960 from small mammals in bulgaria.authors examined a total of 3849 specimens of mammals included in 36 species from different region of bulgaria. adiaspiromycosis was detected microscopically in 18 mammals species. strains of emmonsia crescens emmons et jellison 1960 was isolated from 6 species--apodemus flavicollis, apodemus sylvaticus, clethrionomys glareolus, microtus arvalis, musmusculus and rattus norvegicus.19751171369
expression of a single lignin peroxidase-encoding gene in phanerochaete chrysosporium strain me446.a previously described linked set of lignin peroxidase-encoding genes (lpo) from phanerochaete chrysosporium (p.c.) me446 is not expressed under standard growth conditions for ligninolytic activity. however, a single unlinked lpo gene, not previously described in p.c. strain me446, is expressed. the transcription start points of this gene are mapped and the gene is assigned to a genetic linkage group by the use of restriction-site polymorphism segregation analysis. no transcripts from lpo-relate ...19921351020
extracellular enzyme system utilized by the fungus sporotrichum pulverulentum (chrysosporium lignorum) for the breakdown of cullulose. functional characterization of five endo-1,4-beta-glucanases and one exo-1,4-beta-glucanase.a strong synergistic response was observed between the five endo-1,4-beta-glucanases and the exo-1,4-beta-glucanase obtained from culture solutions of the rot fungus sporotrichum pulverulentum (formerly called chrysosporium lignorum), when these enzymes were allowed to degrade de-waxed cotton and avicel. no synergism was observed if walseth cellulose, an acid-swollen cullulose, was used. if de-waxed cotton was pretreated with endo-1,4-beta-glucanases, the exo-1,4-beta-glucanase enzyme released ...19751225521
the role of polecats of the genus putorius cuvier, 1817 in natural foci of adiaspiromycosis.adiaspiromycosis provoked by the fungus emmonsia crescens was diagnosed in two different species of polecats. in total, 76.2% of steppe polecats (putorius eversmanni) and 35.6% of dark polecats (p. putorius) were infected. the infection was not demonstrated at all in ferrets (p. furo) reared as fur animals. the differences found in the extent and intensity of infection in the two species of wild polecats are explained by different ecological requirements of these related carnivores. the habitat ...19751238314
[human pulmonary adiaspiromycosis: comment on a new case in brittany].pulmonary and human adiaspiromycosis in brittany: a new observation. a young woman is operated on for pulmonary abcess. the histological study the pulmonary tissue around and at a distance from the abcess shows numerous adiaspores of emmonsia crescens emmons & jellison (1960). the culture of the fungus were not possible. the authors discuss the relationship between the fungus and the pulmonary lesions observed in the cases of human adiaspiromycosis.19751238904
adiaspiromycosis in the franklin's ground squirrel, spermophilus franklini, and pika, ochotona princeps, from alberta, canada.pulmonary adiaspiromycosis due to emmonsia crescens was diagnosed in three of six franklin's ground squirrels (spermophilus franklini) captured in rochester, central alberts, canada in the summer of 1971, and in one of 240 pikas (ochotona princeps) collected in southwestern alberta in 1969. granulomas measuring 0.5 to 1 mm in diameter were found in both lungs. these granulomas contained adiaspores measuring 60 to 400 mum in diameter. the disease was not found in six franklin's ground squirrels c ...1976943581
[thielavia heterothallica spec. nov. (abb.1) status conidialis: chrysosporium thermophilum (apinis) von klopotek].thielavia heterothallica spec. nov. is described as a heterothallic, thermophilic fungus with spherical, black, non-ostiolate cleistothecia; elliposidal evanescent asci which contain eight one-celled ellipsoidal ascospores, darkening to deep brown to black, with one germ pore. the conidial state is chrysosporium thermophilum (apinis) von klopotek.1976944025
conversion of adiasporic and mycelial stages of emmonsia crescens examined in the scanning electron microscope.conversion of adiasporic and mycelial stages of the fungus emmonsia crescens, the causative agent of adiaspiromycosis, was observed by a scanning electron microscope. multilocular germination of adiaspores and their surface structures are described.1976945212
adiaspiromycosis in large free living carnivores.adiaspiromycosis was identified in 6 animals in the examination of the lungs of 90 large carnivores. emmonsia crescens (chrysosporium parvum var. crescens) was demonstrated as the causative agent in 5 cases of disease-in the badger (meles meles), the otter (lutra lutra) and the fox (vulpes vulpes). e. parva was demonstrated in the remaining case of disease in a fox. the badger is a new, up to the present unknown host of e. crescens. the sporadic occurrence of adiaspiromycosis in the fox and the ...1976945461
scanning electron microscopy of experimental adiaspiromycosis.adiaspiromycotic granulomas of mice experimentally inoculated with fungus emmonsia crescens emmons et jellison 1960 were examined by scanning electron microscopy. there morphology, surface structure, and germinating adiaspores isolated from granulomas are described.1976945464
[lymph node localization of mycoses]. 19751242118
outdoor airborne dermatophytes and related fungi: a survey in turin (italy).aerial dispersal of propagules, an important mechanism of fungal spread, has not been sufficiently studied for skin-infecting fungi. over a period of one year, we have monitored the distribution of dermatophytes in the air of turin. we have also examined the related aleurioconidic and arthroconidic fungi, which share physiological and morphological characters with dermatophytes and are sometimes considered to cause similar infections. a viable volumetric collector was used. samples were taken ea ...19921291878
de-novo biosynthesis of chlorinated aromatics by the white-rot fungus bjerkandera sp. bos55. formation of 3-chloro-anisaldehyde from glucose.the white-rot fungus bjerkandera sp. bos55 produced de-novo several aromatic metabolites. besides veratryl alcohol and veratraldehyde, compounds which are known to be involved in the ligninolytic system of several other white-rot fungi, other metabolites were formed. these included anisaldehyde, 3-chloro-anisaldehyde and a yet unknown compound containing two chlorine atoms. additionally gc/ms analysis revealed the production of small amounts of anisyl alcohol and 3-chloro-anisyl alcohol. after 1 ...19921299620
the nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete phanerochaete chrysosporium.the nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in phanerochaete chrysosporium was studied. our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pme) of p. chrysosporium. using the total dna of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in reca- e. coli strains while only p12-6 was recovered in reca+ e. coli strains. the results ...19921314140
camp-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete phanerochaete chrysosporium.lignin peroxidases (lips) and manganese-dependent peroxidases (mnps) are major components of the lignin-degrading enzyme system of phanerochaete chrysosporium and typically appear during secondary metabolism. the involvement of camp in the regulation of production of lips and mnps was investigated in this study. production of lips and mnps was preceded by a sharp rise in intracellular camp concentration. addition of atropine, theophylline, or histamine to cultures resulted in a drop in intracell ...19921319067
a comparison of the catalytic properties of cellobiose:quinone oxidoreductase and cellobiose oxidase from phanerochaete chrysosporium.several catalytic properties of the fad enzyme cellobiose:quinone oxidoreductase (cbq) and the heme/fad enzyme, cellobiose oxidase (cbo) have been investigated and compared. dichlorophenol-indophenol was found to be a very good electron acceptor for cellobiose oxidation by both enzymes. the optimal ph value for this oxidation with dichlorophenol-indophenol as a co-substrate was observed around ph 4 for both enzymes. the turnover numbers of this reaction were also very similar. the km values for ...19921321038
cellobiose oxidase from phanerochaete chrysosporium. stopped-flow spectrophotometric analysis of ph-dependent reduction.cellobiose oxidase (cbo) from phanerochaete chrysosporium can utilize dichlorphenol-indophenol (cl2ind) and cytochrome c as effective electron acceptors for the oxidation of cellobiose. however, the ph dependencies of activity for these electron acceptors are significantly different. both compounds act as effective electron acceptors at ph 4.2, whereas only dichlorophenol-indophenol is active at ph 5.9. to explain this discrepancy, the ph dependencies of the reduction rates of fad and heme, resp ...19921321733
effect of white rot basidiomycetes on chemical composition and in vitro digestibility of oat straw and alfalfa stems.five white rot basidiomycetes were evaluated for their potential to improve ruminal degradation of oat straw and alfalfa stems. phanerochaete chrysosporium (pc), scytinostroma galactinum (sg), phlebia tremellosa (pt), phellinus pini (pp), and pholiota mutabilis (pm) were incubated on oat straw and alfalfa stems for 30 d at 28 degrees c and 90% relative humidity. detergent fiber and total fiber components (neutral sugars, uronic acids, klason lignin [kl], and ester- and ether-linked non-core lign ...19921321801
saprophytic and keratinophilic fungi isolated from desert and cultivated soils continuously exposed to cement dust particles in egypt.forty soil samples collected from desert (uncultivated) and cultivated soils, exposed continuously to cement dust were screened for their content of saprophytic and keratinophilic fungi using the hair baiting technique and the dilution plate method. using the hair baiting technique, 21 genera and 31 species were collected from 20 samples of each of desert (15 genera and 21 species) and cultivated (17 genera and 24 species) soils, on sabouraud's dextrose agar. these included chrysosporium keratin ...19921325092
manganese(ii) oxidation by manganese peroxidase from the basidiomycete phanerochaete chrysosporium. kinetic mechanism and role of chelators.manganese oxidation by manganese peroxidase (mnp) was investigated. stoichiometric, kinetic, and mnii binding studies demonstrated that mnp has a single manganese binding site near the heme, and two mniii equivalents are formed at the expense of one h2o2 equivalent. since each catalytic cycle step is irreversible, the data fit a peroxidase ping-pong mechanism rather than an ordered bi-bi ping-pong mechanism. mniii-organic acid complexes oxidize terminal phenolic substrates in a second-order reac ...19921429709
multiple enzymatic pathways involved in the metabolism of glyceryl trinitrate in phanerochaete chrysosporium.a study of glyceryl trinitrate metabolism by a filamentous fungus, phanerochaete chrysosporium, carried out with the 14c-labeled substrate, provides evidence for a multienzymatic system leading to di- and mononitrate derivatives. at least two independent enzymatic activities were detected in the cytosolic fraction: an aerobic glutathione s-transferase activity and an anaerobic nadph-dependent soluble cytochrome p450-like activity. other hemoproteins with enzymatic activities dependent upon the p ...19921326993
1h nmr investigation of manganese peroxidase from phanerochaete chrysosporium. a comparison with other peroxidases.1h nmr spectra at 200- and 600-mhz of manganese peroxidase from phanerochaete chrysosporium and of its cyanide derivative are reported. the spectrum of the native protein is very similar to that of other peroxidases. the assignment of the spectrum of the cyanide derivative has been performed through 1d noe, 2d noesy, and cosy experiments. this protein is very similar to lignin peroxidase, the only meaningful difference being the shift of h delta 2 of the proximal histidine. the spectra of the cy ...19921327129
cellobiose oxidase from phanerochaete chrysosporium as a source of fenton's reagent. 19921327893
production of hydroxyl radical by lignin peroxidase from phanerochaete chrysosporium.the mechanism for the production of hydroxyl radical by lignin peroxidase from the white rot fungus phanerochaete chrysosporium was investigated. ferric iron reduction was demonstrated in reaction mixtures containing lignin peroxidase isozyme h2 (liph2), h2o2, veratryl alcohol, oxalate, ferric chloride, and 1,10-phenanthroline. the rate of iron reduction was dependent on the concentration of oxalate and was inhibited by the addition of superoxide dismutase. the addition of ferric iron inhibited ...19921329659
nucleotide sequence of a gene from phanerochaete chrysosporium that shows homology to the faca gene of aspergillus nidulans.heterologous hybridisation was used to isolate a genomic dna sequence from phanerochaete chrysosporium using the faca (acetyl coa synthetase) gene from aspergillus nidulans as a probe. the cloned sequence hybridises to a 2.2 kb transcript in poly(a)+ rna prepared from mycelium grown on acetate as the sole carbon source. comparison of the dna sequence obtained with those of the a. nidulans faca and n. crassa acu5 genes reveals an orf that appears to be interrupted by five typical fungal introns. ...19921352996
keratinolytic fungi in egyptian soils. i. baited with hair and wool.150 soil samples were collected, 90 from nile valley and delta, 36 from desert and 24 from salt marshes. human, buffalo and cow hair and sheep wool were used as baits at three incubation temperatures. forty-four species which belong to twenty-one genera at 27 degrees c and forty-two which belong to twenty-two genera at 37 degrees c were collected. we isolated the following keratinophilic fungi chrysosporium tropicum, c. keratinophilum, c. indicum, c. pannicola, c. queenslandicum, trichophyton te ...19921471440
screening of basidiomycetes for lignin peroxidase genes using a dna probe.basidiomycetes were screened for lignin peroxidase (lpo) genes using a dna probe prepared from the lpo restriction fragment of phanerochaete chrysosporium. southern blot analysis showed restriction fragments of chromosomal dna of bjerkandera adusta and coriolus consors hybridized with the probe. bjerkandera adusta produced lpo in a glucose-peptone medium. ion-exchange chromatography showed that this fungus produced multiple molecular forms of lpo. one of the enzymes, lpo-2, was purified and char ...19901366392
multiple lignin peroxidases of phanerochaete chrysosporium ina-12.nine proteins with lignin peroxidase activity were separated from cultures of phanerochaete chrysosporium ina-12 in glycerol as carbon source and non-nitrogen limited. four lignin peroxidase isozymes (4, 5, 8, 9) were purified and characterized. although differences in kinetic parameters could be shown, antibody reaction showed homology between isozymes. however, thermal stability studied, peptide mapping results, and n-terminal sequence analyses established a higher degree of homology between i ...19901366630
characteristics and n-terminal amino acid sequence of a manganese peroxidase purified from lentinula edodes cultures grown on a commercial wood substrate.extracellular culture filtrates from ligninolytic cultures of the lignin-degrading basidiomycete lentinula (syn. lentinus) edodes (berk.) pegler contained one major peroxidase when grown on a commercial oak-wood substrate. the peroxidase was purified by polyethylenimine clarification, anion-exchange chromatography, and hydrophobic-interaction hplc. the enzyme (mnp1) was a heme-iron protein with an apparent molecular weight of 44,600 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis ge ...19901366641
[comparison of growth and morphological differentiation of 22 strains of emmonsia ciferri et montemartini, 1959, agent of adiasporomycosis, in different temperatures].the comparative study of the responses of 22 strains of emmonsia to different temperatures between 5 degrees and 40 degrees c allowed us to confirm the existence of thermophilic and non-thermophilic strains. 11 strains composed a very homogeneous non-thermophilic group: their growth, maximal at 20-25 degress c was almost completely inhibited above 30 degrees and they produced characteristic adiaspores at 35 degrees. the remaining 11 strains composed a thermophilic group: their growth was maximal ...19751239665
[first european case of adiaspiromycosis due to emmonsia parva (emmons and ashburn) ciferri and montemartini 1959, in a mammal: apodemus flavicollis]. 19751239973
lipase production by free and immobilized protoplasts of sporotrichum (chrysosporium) thermophile apinis.production of lipase by free and alginate-entrapped protoplasts was studied in batch culture. cell-wall-degrading enzymes novozym 234 and cellulase cp improved lipase secretion of normal mycelium by 25%-100%. the protoplast-regenerated mycelium exhibited several-fold higher lipase activity in batch replacements in tris buffer over normal spore-derived mycelium. the specific lipase activity of immobilized protoplasts was about four times higher than normal mycelial beads. protoplasts beads were s ...19901366741
the 1,4-beta-d-glucan cellobiohydrolases from phanerochaete chrysosporium. i. a system of synergistically acting enzymes homologous to trichoderma reesei.a physico-chemical and structural characterization of three 1,4-beta-d-glucan cellobiohydrolases (ec. 3.2.1.91), isolated from a culture filtrate of the white-rot fungus phanerochaete chrysosporium, reveals that the cellulolytic enzyme secretion pattern and thus the general degradation strategy for p. chrysosporium is similar to that of trichoderma reesei. partial sequence data show that two of the isolated enzymes, i.e., cbhi, pi 3.82 and cbh62, pi 4.85, are homologous with cbhi and egi from t. ...19911367241
[adiaspiromycosis of the polecats]. 19751240597
detoxification of polycyclic aromatic hydrocarbons by fungi.the polycyclic aromatic hydrocarbons (pahs) are a group of hazardous environmental pollutants, many of which are acutely toxic, mutagenic, or carcinogenic. a diverse group of fungi, including aspergillus ochraceus, cunninghamella elegans, phanerochaete chrysosporium, saccharomyces cerevisiae, and syncephalastrum racemosum, have the ability to oxidize pahs. the pahs anthracene, benz[a]anthracene, benzo[a]pyrene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene, as well as several met ...19921367975
occurrence of keratinolytic fungi and related dermatophytes in soils in cairo, egypt.120 soil samples collected from various sites of cairo were processed for the isolation of keratinophilic fungi by "tokava" hair baiting technique. 22 species belonging to 6 genera were isolated viz.: chrysosporium tropicum, c. indicum, c. keratinophilum, c. queenslandicum, c. merdarium, c. anamorph of arthroderma curreyi, c. pannicola, c. lobatum, c. anamorph of renispora flavissima, c. pseudomerdarium, microascus mangini, malbranchea gypsea, ml. state of uncicarpus reesii, ml. state of coccidi ...19921380752
biodegradation of soil humic acids by streptomyces viridosporus.biodegradation of soil humic acids by streptomyces viridosporus atcc 39115 growing in a mineral salts--glucose medium was demonstrated. this biodegradation accompanies bacterial growth and is, therefore, presumed to be a primary metabolic activity, but humic acids were not used as the sole source of carbon. this bacterial activity was enhanced when cells were shaken and within a ph range of 6.5-8.5. in further experiments, the relative abilities of s. viridosporus to mineralized [14c]melanoidin, ...19921393821
production of fenton's reagent by cellobiose oxidase from cellulolytic cultures of phanerochaete chrysosporium.the reduction of dioxygen by cellobiose oxidase leads to accumulation of h2o2, with either cellobiose or microcrystalline cellulose as electron donor. cellobiose oxidase will also reduce many fe(iii) complexes, including fe(iii) acetate. many fe(ii) complexes react with h2o2 to produce hydroxyl radicals or a similarly reactive species in the fenton reaction as shown: h2o2 + fe2+----ho. + ho- + fe3+. the hydroxylation of salicylic acid to 2,3-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid is ...19921396686
homology between cellobiose oxidase from phanerochaete chrysosporium and other proteins. 19921397518
haloperoxidase activity of phanerochaete chrysosporium lignin peroxidases h2 and h8.monochlorodimedone (mcd), commonly used as a halogen acceptor for haloperoxidase assays, was oxidized by hydrogen peroxide in the presence of lignin peroxidase isoenzymes h2 and h8. when oxidized, it produced a weak absorption band with an intensity that varied with ph. this absorbance was used as a simple method for the product analysis because it disappeared when mcd was brominated or chlorinated. we assessed the activity of the lignin peroxidases for oxidation of bromide by measuring the brom ...19921420193
genomic organization of a cellulase gene family in phanerochaete chrysosporium.southern blot and nucleotide sequence analysis of phanerochaete chrysosporium bkm-f-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the trichoderma reesei cellobiohydrolase i gene (cbh1). using pulsed-field gel electrophoresis to separate p. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. the organization of these genes was similar i ...19921423728
an improved method for the purification of lignin peroxidases from phanerochaete chrysosporium ina-12: properties of two major isoforms.1. phanerochaete chrysosporium ina-12 secretes several lignin peroxidase isoenzymes. this paper reports an improved procedure for the purification of the different isoforms compared to those previously described. 2. lignin peroxidases are first concentrated and prefractionated on fast-flow ion-exchangers which avoid concentration by ultrafiltration and dialysis. 3. further purification is achieved by hydrophobic interaction chromatography and anion-exchange fplc. 4. two major forms were purified ...19921426519
a selective technique for isolation of three species of chrysosporium from soils. 19921435853
fungal biodegradation of lignopolystyrene graft copolymers.white rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene [poly(1-phenylethylene)]. the biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4% (wt/wt) lignin. the polymer samples were incubated with the white rot fungi pleurotus ostreatus, phanerochaete chrysosporium, and trametes versicolor and the brown rot fungus gloeophyllum trabeum. w ...19921444360
degradation of phenanthrene by phanerochaete chrysosporium occurs under ligninolytic as well as nonligninolytic conditions.in order to delineate the roles of lignin and manganese peroxidases in the degradation of polycyclic aromatic hydrocarbons by phanerochaete chrysosporium, the biodegradation of phenanthrene (chosen as a model for polycyclic aromatic hydrocarbons) was investigated. the disappearance of phenanthrene from the extracellular medium and mycelia was determined by using gas chromatography. the disappearance of phenanthrene from cultures of wild-type strains bkm-f1767 (atcc 24725) and me446 (atcc 34541) ...19921444413
enhanced biodegradation of phenanthrene in oil tar-contaminated soils supplemented with phanerochaete chrysosporium.in recent years, the white rot fungus phanerochaete chrysosporium has shown promise as an organism suitable for the breakdown of a broad spectrum of environmental pollutants, including polynuclear aromatic hydrocarbons (pahs). the focus of this study was to determine whether p. chrysosporium could effectively operate in an actual field sample of oil tar-contaminated soil. the soil was loaded with [14c]phenanthrene to serve as a model compound representative of the pahs. soil with the native flor ...19921444426
influence of 2,4,6-trinitrotoluene (tnt) concentration on the degradation of tnt in explosive-contaminated soils by the white rot fungus phanerochaete chrysosporium.the ability of phanerochaete chrysosporium to bioremediate tnt (2,4,6-trinitrotoluene) in a soil containing 12,000 ppm of tnt and the explosives rdx (hexahydro-1,3,5-trinitro-1,3,5- triazine; 3,000 ppm) and hmx (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine; 300 ppm) was investigated. the fungus did not grow in malt extract broth containing more than 0.02% (wt/vol; 24 ppm of tnt) soil. pure tnt or explosives extracted from the soil were degraded by p. chrysosporium spore-inoculated cultures a ...19921444437
mineralization of sulfonated azo dyes and sulfanilic acid by phanerochaete chrysosporium and streptomyces chromofuscus.five 14c-radiolabeled azo dyes and sulfanilic acid were synthesized and used to examine the relationship between dye substitution patterns and biodegradability (mineralization to co2) by a white-rot fungus and an actinomycete. 4-amino-[u-14c]benzenesulfonic acid and 4-(3-sulfo-4-aminophenylazo)-[u-14c]benzenesulfonic acid were used as representative compounds having sulfo groups or both sulfo and azo groups. such compounds are not known to be present in the biosphere as natural products. the int ...19921482182
influence of aromatic substitution patterns on azo dye degradability by streptomyces spp. and phanerochaete chrysosporium.twenty-two azo dyes were used to study the influence of substituents on azo dye biodegradability and to explore the possibility of enhancing the biodegradabilities of azo dyes without affecting their properties as dyes by changing their chemical structures. streptomyces spp. and phanerochaete chrysosporium were used in the study. none of the actinomycetes (streptomyces rochei a10, streptomyces chromofuscus a11, streptomyces diastaticus a12, s. diastaticus a13, and s. rochei a14) degraded the com ...19921482183
nucleotide sequence of a new lignin peroxidase gene glg3 from the white-rot fungus, phanerochaete chrysosporium. 19921508705
characterization of a highly expressed lignin peroxidase-encoding gene from the basidiomycete phanerochaete chrysosporium.the genomic clone, lg2, encoding lip2, the major lignin peroxidase (lip) isozyme from phanerochaete chrysosporium strain ogc101, was isolated and characterized. the 5'-untranslated region of lg2 contains sequences similar to cre and xre promoter elements. comparison with its transcript indicates that eight introns, each less than 59 bp, interrupt the coding sequence. comparison with genes encoding other lip isozymes shows five related patterns of intron location, whose incidence coincides with d ...19921511887
degradation of azo dyes by the lignin-degrading fungus phanerochaete chrysosporium.under nitrogen-limiting, secondary metabolic conditions, the white rot basidiomycete phanerochaete chrysosporium extensively mineralized the specifically 14c-ring-labeled azo dyes 4-phenylazophenol, 4-phenylazo-2-methoxyphenol, disperse yellow 3 [2-(4'-acetamidophenylazo)-4-methylphenol], 4-phenylazoaniline, n,n-dimethyl-4-phenylazoaniline, disperse orange 3 [4-(4'-nitrophenylazo)-aniline], and solvent yellow 14 (1-phenylazo-2-naphthol). twelve days after addition to cultures, the dyes had been ...19921514787
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