| aminoacylase pellets. | aminoacylase was immobilized on the mycelium pellets of aspergillus ochraceus by using albumin and glutaraldehyde. no difference in the optimum ph was observed between native aminoacylase and aminoacylase pellets. the aminoacylase pellets were stable in ph 4-8 but they were unstable in alkaline conditions. the aminoacylase pellets were more stable against heavy metal ions and inhibitors than native aminoacylase. however, the degree of the activation of aminoacylase with cobalt ion decreased with ... | 1977 | 14748 |
| 6-mfa, an antiviral agent from aspergillus ochraceus atcc 28706: influence of body weight and mineral oil administration of the antiviral activity in mice. | sera obtained from mice treated with 6-mfa, an antiviral agent from aspergillus ochraceus atcc 28706 showing high interferon activity, could be transferred to healthy animals to make them resistant to semliki forest virus (sfv) infection. body weight of mice directly influenced, within limits, the proportion of treated mice surviving challenge virus infection as well as interferon production. mineral oil (liquid paraffin, b. p.) administered prior to 6-mfa increased both the level of interferon ... | 1977 | 15439 |
| influence of solute and hydrogen ion concentration on the water relations of some xerophilic fungi. | germination and growth of six xerophilic fungi, aspergillus flavus, aspergillus ochraceus, eurotium chevalieri, chrysosphorium fastidium, wallemia sebi and xeromyces bisporus were examined on media of a wide range of water activities (aw). the influence of three solutes, nacl, glycerol and a glucose/fructose mixture, was studied at ph 4-0 and ph 6-5 using a plate-slide technique. germination times and growth rates were affected by solute type, but the influence of ph was less marked. except for ... | 1977 | 19558 |
| purification and properties of rennin-like enzyme from aspergillus ochraceus. | an active milk-clotting enzyme was purified some 40-fold from culture supernatant of aspergillus ochraceus. the purification steps included ammonium sulfate precipitation, g-100 sephadex gel filtration, and ion exchange chromatography, using deae cellulose column. the enzyme exhibited milk-clotting activity and proteolytic behaviour, an optimum at ph 6.0 and in the range of 7--8.5, respectively. the purified enzyme was actively proteolytic against casein, haemoglobin, and bovine serum albumin at ... | 1978 | 31745 |
| [proteolytic enzymes produced by aspergillus ochraceus in relation to their plasma coagulating and fibrinolytic activities]. | the paper describes some properties of coagulases and fibrinolytic enzymes isolated from a proteolytic complex produced by aspergillus ochraceus hp-19 during submerged cultivation on a synthetic medium. proteolytic enzymes with the plasmocoagulating activity hydrolyzed casein at the maximum rate at 45 degrees c and ph 8.0--10.0. the coagulases were stable at ph 5.0--7.0 and were rather resistant to low ph values. the enzymes were entirely inactivated at 55 degrees c within 20--30 min. the activi ... | 1979 | 41165 |
| allergic bronchopulmonary aspergillosis caused by aspergillus ochraceus. | a case with the characteristics of allergic bronchopulmonary aspergillosis is described. the species of aspergillus involved, a. ochraceus, has not previously been found in this disorder. the organism had antigenic properties distinct from five other species of aspergillus most commonly associated with allergic bronchopulmonary aspergillosis. the patient had immediate skin test, immunodiffusion, and radioallergosorbent reactivity to the species. four per cent of 112 serum samples from others sus ... | 1978 | 102184 |
| metabolism of glutamic acid in aspergillus ochraceus during the biosynthesis of ochratoxin a. | the uptake and utilization of glutamic acid in the biosynthesis of ochratoxin a by aspergillus ochraceus were studied. uniformly labeled l[14c]glutamic acid was incorporated into both the phenylalanine and isocoumarin moieties of ochratoxin a. penicillic acid was also labeled. during the early stages of development, the amino acid was used mainly for the synthesis of ribonucleic acid and protein. a portion of glutamic acid was oxidized and was recovered as metabolic 14co-2. the initial uptake v ... | 1975 | 234718 |
| [animal experiments on the pharmacokinetics of ocrase (author's transl)]. | the blood level, distribution and elimination of ocrase, a protease from aspergillus ochraceus, were determined in rabbits after application of the 131i labelled enzyme in therapeutic doses. | 1979 | 397495 |
| characterization of rennin-like enzyme produced in submerged cultures of aspergillus niger. | | 1979 | 400533 |
| mycotoxicosis produced in swine by cultural products of an isolate of aspergillus ochraceus. i. clinical observations and pathology. | pigs fed a ration, 25% of which was rice culture, of aspergillus ochraceus lost weight or failed to gain and became depressed. some pigs died and most developed subcutaneous edema, hydrothorax, hydroperitoneum, pulmonary atelectasis, edema of the mesentery and perirenal edema. microscopic lesions in addition to edema were primarily renal and consisted of tubular degeneration and necrosis, hyaline tubular casts, interstitial fibrosis and tubular cell regeneration. the first change found after 3 d ... | 1979 | 473489 |
| [proteolytic system of aspergillus ochraceus studied in relation to its plasma-coagulating and fibrinolytic activities]. | the culture of aspergillus ochraceus hp-19 accumulates two types of proteolytic enzymes in the cultural broth. one group of proteases coagulates blood while the other has the fibrinolytic activity. the enzymes can be separated by gel filtration on sephadex g-100. the first group of enzymes consists of several proteases with the coagulase activity and an inert protein. | 1979 | 481278 |
| mycotoxicosis produced in swine by cultural products of an isolate of aspergillus ochraceus. ii. clinicopathologic changes. | rice culture of a toxigenic strain of aspergillus ochraceus was fed at a concentration of 25% to weanling pigs for 10 days. the clinicopathological abnormalities reflected renal damage. activities of lactic dehydrogenase, glutamic-oxaloacetic transaminase and isocitrate dehydrogenase were increased in the urine but not in the serum. serum concentrations of urea nitrogen and creatinine were high. cellular and granular casts, blood, protein, and glucose were in the urine of pigs fed toxic diet. se ... | 1979 | 505895 |
| [moulds of bakery dusts (author's transl)]. | a total of 260 samples from bakery dusts were investigated. the samples were taken in the great part with the sedimentation-method. a large concentration of moulds was found in the meal-room. the fungal flora was here charcterized by species of rhizopus nigricans, mucor and circinella. with a new volumetric sampler a total quantity of 8 680 fungus spores of particles in 1 m3 was registrated. by a re-examination we found 4473. in the baker's rooms besides moulds (aspergillus, penicillium etc.) th ... | 1977 | 558701 |
| mycotoxic diseases produced in mice by species of the aspergillus ochraceus group. | | 1977 | 598791 |
| [animal experimental studies on the thrombolytic effect of ocrase]. | the thrombolytic effect of "ocrase", a protease isolated from aspergillus ochraceus, was investigated in animal experiments. to prevent general hyperproteolysis a protease dose was used which was was neutralized by the inhibitors in the blood of the animals. experimentally produced deposition and clotting thrombi in peripheral venous or arterial vessels of mice, rats, dwarf pigs and dogs were lysed by regional and systemic administration, and the therapeutic result was estimated by vital microsc ... | 1977 | 614755 |
| effects in mice of simultaneous prenatal exposure to ochratoxin a and t-2 toxin. | aspergillus ochraceus and fusarium tricinctum are food contaminating molds whose toxic metabolites, ochratoxin a and t-2 toxin, are known mammalian teratogens. in order to determine the possible effects of simultaneous exposure to such environmental agents, ochratoxin a (2 or 4 mg/kg) and t-2 toxin (0.5 mg/kg) were injected ip, either together or individually, in cd-1 mice on gestation days 8 or 10. ochratoxin induced craniofacial malformations when given alone on day 8, but not on day 10. t-2 t ... | 1978 | 625706 |
| studies on growth inhibition by lectins of penicillia and aspergilli. | it has previously been shown in our laboratory that wheat germ agglutinin (wga) binds to trichoderma viride and inhibits growth of this fungus. here we report on the effect of wga, soybean agglutinin (sba) and peanut agglutinin (pna) on penicillia and aspergilli. binding of the lectins to the fungi was examined with the aid of their fluorescein isothiocyanate (fitc) conjugated derivatives. fitc-wga bound to young hyphal walls of all species, in particular to the hyphal tips and septa, in agreeme ... | 1978 | 637658 |
| antiviral agent (6-mfa) from aspergillus ochraceus--sensitivity of arboviruses in experimentally infected mice. | | 1978 | 680873 |
| production of xanthomegnin and viomellein by isolates of aspergillus ochraceus, penicillium cyclopium, and penicillium viridicatum. | fungal isolates from legumes were cultured on rice and examined for production of the toxic mold metabolites xanthomegnin and viomellein. six of 14 aspergillus ochraceus isolates produced from 0.3 to 1.3 mg of xanthomegnin per g and 0.1 to 1.0 mg of viomellein per g. one of nine isolates of penicillium cyclopium produced 0.1 mg of xanthomegnin per g and 0.06 mg of viomellein per g. three of nine p. viridicatum isolates produced from 0.4 to 1.6 mg of xanthomegnin per g and 0.2 to 0.4 mg of viomel ... | 1978 | 708029 |
| mycotoxicosis produced in rats by cultural products of an isolate of aspergillus ochraceus. | | 1978 | 711069 |
| production of xanthomegnin and viomellein by species of aspergillus correlated with mycotoxicosis produced in mice. | by using thin-layer chromatography and infrared spectroscopy, xanthomegnin and viomellein have been isolated and identified from species of the aspergillus ochraceus group. a correlation was established between the occurrence of these fungal quinones in the fungal cultural products and the ability of these products to induce mycotoxicosis in mice. in addition, a method was employed to estimate the amount of xanthomegnin and viomellein produced by the fungi. | 1978 | 736540 |
| an improved 11 alpha-hydroxylation of progesterone by aspergillus ochraceus ts. | 1. the stereospecific hydroxylation of progesterone exclusively to its 11 alpha-hydroxy derivative by aspergillus ochraceus ts is reported. 2. there is no secondary metabolite (6beta, 11 alpha-dihydroxyprogesterone) formed even when the transformation was attempted with different concentrations of the substrate (200 microgram/ml-200 mg/ml) for prolonged with zn2+ at a concentration of 50 microgram/ml identical results were obtained. 3. similar results were also obtained at different ph values of ... | 1978 | 743260 |
| production of ochratoxin a by aspergillus ochraceus nrrl-3174 before and after exposures to 60co irradiation. | spores from the toxigenic organism aspergillus ochraceus nrrl-3174 were exposed to specific levels of gamma irradiation and then allowed to germinate on selected media. increases in ochratoxin a production by irradiated, compared to non-irradiated, spores were observed after inoculation of spores onto a cracked red wheat or into a synthetic liquid medium. variations in daily ochratoxin production were also observed for control and irradiated spore-derived cultures developing on both media, with ... | 1976 | 938031 |
| mycotoxicosis produced in mice by cultural products of an isolate of aspergillus ochraceus. | | 1976 | 1017773 |
| [thrombolytic and pharmacodynamic properties of aspergillus ochraceus protease]. | the protease isolated from aspergillus ochraceus exerts a strong thrombolytic action. in in vitro experiments and in tests with animals (mice, rats, dogs) it caused lysis of fibrinous clots. the thrombolytic effect of the protease is conditioned by its direct influence on the fibrin. besides the thrombolytic action the protease, used within a definite ranges of doses, displays antithrombotic properties. the paper cites data on the acute toxicity (in mice, rats and rabbits) and on the influence o ... | 1975 | 1084284 |
| rapid method for preparing cell-free extracts of aspergillus ochraceus. | a rapid method for preparing cell-free extracts of aspergillus ochraceus was developed. mycelial mats were prefrozen in liquid nitrogen, ground to a fine powder in a cold mortar, and homogenized in an all-glass mechanical homogenizer. this method provided preparations averaging 43.0 mg of protein per g of mycelium (wet weight). the method was fast, efficient, and did not subject the extract to temperatures above 1 c or to heavy metals. the preparation method was suitable for studying a varie ... | 1975 | 1115500 |
| [aspergillus ochraceus wilhelm toxins. iv. -- toxicity in rat by prolonged oral administration of ochratoxin a (author's transl)]. | adult male and female rats are given oral doses of 0.25 to 8 mg/kg of ochratoxin a daily for 10 days. this study confirms the intoxication symptomatology and weight loss observed in rat and mouse after sole administration of toxins. estimation of the dl c50 and the calculation of the detoxication coefficient show accumulation of ochratoxin a in the organism. microscopic lesions emphasize ochratoxin a aggression on hepatocytes, nephrocytes and megacaryocytes. serious renal failure may cause the a ... | 1975 | 1163963 |
| microbiological hydroxylation. part xvi. incubation of derivatives (mainly acetals) of 5alpha-androstane ketones with the fungi calonectria decora, aspergillus ochraceus, and rhizopus nigricans. | | 1975 | 1172504 |
| [formation of fatty acids by aspergillus ochraceus 15b growing on a hexadecane containing medium]. | | 1975 | 1214637 |
| microbiological hydroxylation. part xxi. hydroxylations of 3-halogeno-17-oxo-, 3-halogeno-7-oxo-, and 17-halogeno-3-oxo-androstanes by the fungi calonectria decora, rhizopus nigricans, and aspergillus ochraceus. | | 1975 | 1239450 |
| studies on genetic transcription. ii. induced production of new morphological traits in aspergillus ochraceus wilhelm by 2-amino-pyrimidine. | | 1975 | 1243839 |
| ochratoxigenicity of aspergillus ochraceus group and penicillium verrucosum var. cyclopium strains on various media. | production of ochratoxin a (oa) by strains of aspergillus ochraceus group (a. ochraceus--21, a. sclerotiorum--1, a. sulphureus--1) and penicillium verrucosum var. cyclopium strains--11, on various media was investigated. thirteen percent of a. ochraceus strains and 18% of p. verrucosum var. cyclopium formed oa growing on sterile crushed wheat for 14 days at 25 to 27 degrees c (preliminary experiments). toxin concentrations were 5.0 to 7.0 micrograms/kg. five strains oa-positive on crushed wheat ... | 1992 | 1343939 |
| detoxification of polycyclic aromatic hydrocarbons by fungi. | the polycyclic aromatic hydrocarbons (pahs) are a group of hazardous environmental pollutants, many of which are acutely toxic, mutagenic, or carcinogenic. a diverse group of fungi, including aspergillus ochraceus, cunninghamella elegans, phanerochaete chrysosporium, saccharomyces cerevisiae, and syncephalastrum racemosum, have the ability to oxidize pahs. the pahs anthracene, benz[a]anthracene, benzo[a]pyrene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene, as well as several met ... | 1992 | 1367975 |
| new diketopiperazine metabolites from the sclerotia of aspergillus ochraceus. | three new diketopiperazine-containing metabolites 1-3 have been isolated from the sclerotia of aspergillus ochraceus (nrrl 3519) by chromatography on sephadex lh-20 and reversed-phase hplc. the structures of these compounds were established using extensive high-field 1d and 2d nmr experiments. all three compounds cause moderate reduction in weight gain in assays against the lepidopteran crop pest helicoverpa zea. | 1992 | 1402958 |
| [inhibition of eight natural foods on mutagenic effect by aflatoxin b1 and extracts of fungi]. | eight natural foods were tested for their antimutagenic activities on afb1, metabolic extracts of a. versicolor and a. ochraceus which induced mutagenic activity in ta 98 and ta 100 mutants. the tested substances were extracted repeatedly with acetone. the revertance induced by afb1, metabolic extracts of a. versicolor and a. ochraceus were significantly decreased when the eight natural foods were added into the medium. the results showed that the eight natural foods had different degrees of ant ... | 1992 | 1451583 |
| isolation and structure determination of natural analogues of the mycotoxin ochratoxin a produced by aspergillus ochraceus. | three new natural mycotoxins, analogues of ochratoxin a, in which the phenylalanine moiety is replaced by serine, hydroxyproline or lysine, were isolated from cultures of aspergillus ochraceus by tlc followed by hplc column chromatography. their structures were determined after acidic hydrolysis by the characterisation of both their amino acid moieties and of ochratoxin alpha, the chlorinated dihydroisocoumarin moiety of ochratoxin a. these structures were confirmed by mass spectrum analysis. | 1992 | 1471160 |
| mycotoxic flora and mycotoxins in smoke-dried fish from sierra leone. | examination of 20 samples of smoke-dried fish of the ethmolosa sp. commonly called "bonga", from homes and markets in njala (sierra leone) revealed the presence of 4 aspergilli species: a. flavus links ex fries, a. ochraceus wilhelm, a. tamarii kita and a. niger van tieghem. fresh or properly preserved smoke-dried fish showed no signs of fungal contamination. mouldy fish extracts contained varying amounts of aflatoxins b1, g1, g2 and ochratoxin a. isolates of a. flavus grown on yeast extract suc ... | 1992 | 1480216 |
| characterization of a novel microsomal glutathione s-transferase produced by aspergillus ochraceus ts. | purification and characterization of microsomal glutathione s-transferase produced by aspergillus ochraceus ts are reported. the isozymes are located in microsomes and were active against 1-chloro-2,4-dinitrobenzene, ethacrynic acid, 1,2-dichloro-4-nitrobenzene, trans-4-phenyl-3-buten-2-one,p-nitrobenzyl chloride and bromosulphophthalein. they were inhibited by n-ethylmaleimide and bromosulphophthalein. the gst isozymes produced by aspergillus ochraceus ts are indistinguishable in respect of the ... | 1992 | 1488053 |
| production of ochratoxins by some egyptian aspergillus strains. | in all the investigated aspergillus ochraceus and a. alliaceus strains the maximum quantities of ochratoxins produced on yeast extract-sucrose (yes) medium exceeded that detected on wheat solid medium except a. ochraceus 67. the shake cultures lowered ochratoxins formation. the maximum yields of ochratoxins did not coincide with maximum fungal growth. in most a. strains investigated, the total ochratoxin contents of the culture filtrates highly exceeded that of the mycelia. | 1992 | 1509830 |
| use of electron beam irradiation for mold decontamination on cheddar cheese. | cheddar cheese slices, surface-inoculated with either penicillium cyclopium or aspergillus ochraceus spores, were vacuum packaged and irradiated using an electron beam accelerator. following treatment at .21 and .52 kgy, the shelf-life of cheese containing p. cyclopium was extended by 3 and 5.5 d, respectively, in comparison with inoculated, untreated samples. under similar treatment and storage conditions, cheese containing a. ochraceus exhibited average shelf-life extensions of 42.5 and 52.2 d ... | 1992 | 1541727 |
| production of an extracellular milk-clotting activity during development in myxococcus xanthus. | we describe here an extracellular proteolytic activity secreted during both growth and submerged development by myxococcus xanthus dk1622. this activity yields the clotting of kappa-casein at ph 6 and is inhibited by specific inhibitors of aspartic proteases. secretion of this milk-clotting proteolytic activity (of mcp) is time regulated during the developmental cycle, with a large increase near 9 h poststarvation, but its production does not require cell-cell contact. the lack of secretion of t ... | 1992 | 1629171 |
| mixed cultivation of trichoderma reesei and aspergillus ochraceus for improved cellulase production. | aspergillus ochraceus imi 317911 was screened to be a high cellobiase producing strain (2.40 iu/ml). the mixed batch cultivation of trichoderma reesei qm 9414/rut c-30 and a. ochraceus imi 317911 resulted in a balanced enzyme as compared to singly grown cultures. the milled rice straw (6%, w/v) as carbon source was found suitable for production of cellulases resulting in a filter paper activity (fpa) of 1.83 iu/ml and a cellobiase production of 1.63 iu/ml after 7 days of stirred cultivation. an ... | 1992 | 1632200 |
| development of gastric proteases in fetal pigs and pigs from birth to thirty six days of age. the effect of adrenocorticotropin (acth). | development of the synthesis and secretion of gastric proteases was studied in 55 large white x landrace pigs from 22 days before birth (93 days gestation) to 36 days of age. the pigs came from eight litters and were 0.4 - 13.5 kg body weight. littermate pairs were treated with either saline or adrenocorticotropin (acth) from three days of age. secretion of protease activity (milk-clotting and general proteolytic activity) was investigated in anaesthetized pigs by a gastric perfusion technique u ... | 1991 | 1667404 |
| variants of aspergillus alutaceus var. alutaceus (formerly aspergillus ochraceus) with altered ochratoxin a production. | the present studies, using aspergillus alutaceus var. alutaceus berkeley et curtis (formerly a. ochraceus wilhelm) nrrl 3174 along with three other wild-type strains, were undertaken in an attempt to understand the effects of irradiation and other treatments on mycotoxin production in grain. bedford barley was inoculated with spores of nrrl 3174, gamma irradiated, and incubated at 28 degrees c and 25% moisture. after 10 days of incubation, two colony types, ochre (parental) and yellow (variant), ... | 1991 | 1768122 |
| role of the competitive microbial flora in the radiation-induced enhancement of ochratoxin production by aspergillus alutaceus var. alutaceus nrrl 3174. | the radiation sensitivity and the toxigenic potential of conidiospores of the fungus aspergillus alutaceus var. alutaceus were determined after irradiation with 60co gamma rays and high-energy electrons. over the ph range of 3.6 to 8.8, the doses required for a 1 log10 reduction in viability based on the exponential portion of the survival curve ranged from 0.21 to 0.22 kgy, with extrapolation numbers (extrapolation of the exponential portion of the survival curve to zero dose) of 1.01 to 1.33, ... | 1991 | 1768123 |
| [the separation of true coagulases from other enzymes of the extracellular proteolytic complex of aspergillus ochraceus by using affinity chromatography]. | a complex of proteases has been isolated from the cultural broth of fungi aspergillus ochraceus hp-19 by precipitation ammonium sulfate. using the method of affinity chromatography on the biospecific sorbents the conditions for separation of proteases into ones with coagulase activity and with fibrinolytic activity have been found. | 1991 | 1768732 |
| novel metabolite structures from biotransformation of a sesquiterpenoid ketone by selected fungal strains. | the sesquiterpenoid ketone, 1,4,4-trimethyltricyclo[5.4.0.0(3.5)]undec-7-en-9-one (1), was subjected to microbial transformation by six fungal strains: aspergillus niger atcc 9142, aspergillus ochraceus dsm 824, beauveria bassiana atcc 7159, cunninghamella echinulata atcc 9244, rhizopus arrhizus atcc 11.145, and absidia blakesleeana atcc 10.148. four main metabolites were formed from 1: 10(r)- and 10(s)-hydroxy-1,4,4-trimethyltricyclo-[5.4.0.0(3.5)]undec-7- en- 9-one (2 and 3, respectively), bes ... | 1991 | 1872996 |
| evaluation of microbial metabolites for trypanocidal activity: significance of biochemical and biological parameters in the mouse model of trypanosomiasis. | trypanosomiasis is a parasitic disease, prevailing in both humans and animals, caused by a single-cell parasite, trypanosoma spp. three microbial metabolites, namely antiamoebin, f-857 and 6-mfa, were evaluated for trypanocidal activity by using a mouse model of trypanosomiasis, which is caused by t. evansi. the significance of the biological and biochemical parameters with respect to physio-pathology of trypanosomiasis and their implications in the evaluation of new trypanocidal compounds were ... | 1991 | 1875553 |
| sixth mycelial fraction acetone (6-mfa), an interferon inducer modulates acrylamide neurotoxicity. | a single i.p. administration of an immunomodulatory agent 6-mfa (a biological response modifier and antiviral agent of fungal origin, 10 mg/100g b.wt.), on 5th day of repeated acrylamide (acr, 50 mg/kg b.wt.) treatment significantly protected rats against its specific neurotoxic effects. corpus striatal 3h-spiperone binding elevated (24%) while glutathione-s-transferase (gst) activity decreased (33%) in acr group but values were markedly restored in 6-mfa alone and co-exposed group. development ... | 1991 | 1882702 |
| protective effect of fungal growth product (6mfa), assessed therapeutically against ehrlich's ascites tumor in swiss mice. | 6mfa is a growth product of the fungus aspergillus ochraceus (atcc 28706) obtained by fermentation in stationary culture. it has both interferon inducing and antiviral properties, in vivo and in vitro, with a relatively high margin of safety (9, 17, 18). ehrlich's ascites tumor bearing swiss albino male mice were treated with 0.5 ml of acqueous preparation of 6mfa (0.75 mg total solids) i.p. in a therapeutic regimen schedule; the sham treated mice received only pbs. 6mfa treatment produced an in ... | 1991 | 1897382 |
| mycotoxin production on different cultivars and lines of broad bean (vicia faba l.) seeds in egypt. | one hundred different cultivars and lines of broad bean (vicia faba l.) seed samples were inoculated with aspergillus flavus link (cmi 102135) to determine varietal differences which may support or resist aflatoxin production. thin-layer chromatographic analysis of the chloroform extracts of the different seed samples revealed that 11 cultivars/lines were highly resistant to seed invasion and aflatoxin production while 9 cultivars/line showed partial resistance. the remaining 80 samples were sus ... | 1991 | 1906134 |
| protective effect of 6-mfa, a fungal interferon inducer against japanese encephalitis virus in bonnet macaques. | 6-mfa, an extract from the fungus aspergillus ochraceus was administered to 8 bonnet macaques. an equal number of monkeys matched for age, sex and weight received placebo and served as controls. twenty hours after the administration of the 6-mfa/placebo the monkeys were challenged with an indian strain of japanese encephalitis virus by the intranasal route. signs and symptoms of the disease such as fever, tremors, loss of appetite, dehydration, flaccid paraplegia or quadriplegia were pronounced ... | 1990 | 1965430 |
| the activity of aspergillus ochraceus (fungi) on replete females of rhipicephalus sanguineus (acari: ixodidae) in natural and experimental conditions. | aspergillus ochraceus wilhelm was isolated from rhipicephalus sanguineus (latreille) ticks infected under natural conditions, and developing an illness characterized by absence of oviposition, mummification and death. from the histological sections, it is deduced that a. ochraceus colonized the ticks via the anus, as posterior gut and malpighian tubules are invaded. the experimental inoculation was carried out on replete female ticks; a disease alike to that observed in naturally infected ticks ... | 1990 | 2093613 |
| ochratoxinogenicity of aspergillus ochraceus strains from nephropathic and non-nephropathic areas in yugoslavia. | mycological analyses of 855 samples of stored grains and dried meat collected in period 1980-1987 from individual households in the nephropathic and wider non-nephropathic area in sr croatia in yugoslavia showed 10% of samples to be contaminated with aspergillus ochraceus. ability to produce ochratoxin a (oa) was tested in 70 samples (27 from nephropathic areas and 43 from non-nephropathic areas). the detection was carried out under uv-light (365 nm) (light blue fluorescence) and 6 oa-producers ... | 1990 | 2366853 |
| mould growth on black table olives and prevention by sorbic acid, methyl-eugenol and spice essential oil. | thirty-two mould strains were isolated from turkish-style black table olives. four of these isolates were identified as aspergillus ochraceus, two as a. niger, 16 as penicillium clavigerum and 10 as p. expansum. mould growth was evaluated on olives treated with 500 ppm sorbic acid, 1000 ppm methyl-eugenol and 1500 ppm çörtük spice (echinophora sibthorpiana guss.) essential oil. olives inoculated with a. parasiticus nrrl 2999 and p. clavigerum were also examined. all samples were stored at 28 deg ... | 1990 | 2388689 |
| neurochemical effects of prenatal treatment with ochratoxin a on fetal and adult mouse brain. | ochratoxin a (oa) is a mycotoxin produced by several storage fungi, such as aspergillus ochraceus and several penicillium species. oa (3 mg/kg) was given intraperitoneally to pregnant mice on day 11 of gestation (day 1 = day of insemination), and neurochemical changes in brains of their offspring were examined at fetal and adult stages. oa treatment produced retardation of intrauterine growth as well as microencephaly and reductions in total weight and dna content of fetal brains. specific activ ... | 1988 | 2467220 |
| hobson's choice. | | 1989 | 2610943 |
| protective effect of 6-mfa, an interferon inducer against acrylamide neurotoxicity. | protective effect of 6-mfa, an interferon inducer and antiviral agent of fungal origin, was investigated against the neurotoxic effects induced by acrylamide in rats. animals of 6-mfa (2.5, 5, 10 mg/100 gm, i.p.) pretreated plus acrylamide (acr) group exhibited a reduction in development of hind limb paralysis which was 34, 25 and 20 (%) with increasing doses of 6-mfa respectively. corpus striatal dopamine binding was significantly raised in the acr treated rats while 6-mfa (10 mg) plus acr grou ... | 1989 | 2816492 |
| role of siderophores in iron storage in spores of neurospora crassa and aspergillus ochraceus. | spores of neurospora crassa 74a are lacking in ferritinlike iron pools, as demonstrated by mössbauer spectroscopic analysis. the cyclic hexapeptide siderophore ferricrocin constituted 47% of the total iron content in spores. after germination and growth, the ferricrocin iron pool disappeared, indicating that the metal was utilized. in spores of aspergillus ochraceus, 74% of the total iron content was bound by ferrichrome-type siderophores. siderophores may function as iron storage forms in funga ... | 1987 | 2960664 |
| mycotoxin production by molds isolated from 'greek-style' black olives. | seventeen mold strains were isolated from 'greek-style' black olives produced in morocco. eight of these isolates were identified as aspergillus flavus, seven as aspergillus petrakii, and two as aspergillus ochraceus wilhelm. the a. flavus strains were tested for production of aflatoxins b1, b2, g1, and g2; and a. ochraceus and a. petrakii strains were tested for production of ochratoxin, penicillic acid, patulin, and citrinin. the organisms were tested for mycotoxin production on five different ... | 1988 | 3079463 |
| mould spoilage and mycotoxin formation in grains as controlled by physical means. | low oxygen concentrations (less than 1%) and/or increased concentrations of co2 or n2 have been found to be highly effective in preventing the development of mould on grain and in inhibiting selected mycotoxins, e.g. aflatoxins, ochratoxin, patulin, penicillic acid and t-2. however, the levels of co2 needed to inhibit mould growth are much higher than those required for the inhibition of mycotoxin production. the degree of inhibition achieved by elevated co2 concentrations is dependent on other ... | 1988 | 3079472 |
| [preparation of the mycotoxin ochratoxin a for research and diagnostic purposes]. | the following fungal strains were tested for the production of the mycotoxin ochratoxin a: aspergillus melleus (ccm f 802), aspergillus ochraceus (ccm 8002) and aspergillus ochraceus (ccm f 803). the strain aspergillus melleus (ccm f 802) proved to be very suitable for the laboratory preparation of ochratoxin a. the mould was multiplied on a natural substrate for 14 days at the temperature of 28 degrees c and at 100% relative humidity. ochratoxin a was extracted into organic solvents (chloroform ... | 1987 | 3125655 |
| seedborne fungi of sesame (sesamum indicum l) in sierra leone and their potential aflatoxin/mycotoxin production. | sesame seeds (sesamum indicum l) from four different geographic locations in sierra leone were sampled for their mycoflora. three toxigenic aspergillus species: a. flavus link ex fries, a. ochraceus wilhelm, and a. tamarii kita were common to all samples. penicillium citrinum thom and two fusarium sp. were found in samples from two localities. the mycotoxins aflatoxin b1 and g1, ochratoxin a and b, and citrinin were positively identified. | 1988 | 3146700 |
| l-657,398, a novel antifungal agent: fermentation, isolation, structural elucidation and biological properties. | l-657,398 is a broad spectrum antifungal agent isolated from solid fermentation or from the mycelium of the liquid fermentation of aspergillus ochraceus. structurally, the compound is a novel pyrollidine related to anisomycin. | 1988 | 3209471 |
| changes during lactation in the renin-like enzyme concentration in rat luteal tissue. | the activity of a renin-like enzyme (rle) previously found in rat copora lutea was studied during lactation. luteal rle concentration significantly increased after delivery and reached a maximum on day 5 of lactation. plasmatic levels of prl and progesterone also increased through lactation. treatment with 2 bromo-alpha-ergocryptine, which diminished plasma prl and progesterone levels, enhanced luteal rle activity. therefore, the increase in luteal rle during lactation seems to be independent of ... | 1987 | 3321821 |
| secretion of mucor rennin, a fungal aspartic protease of mucor pusillus, by recombinant yeast cells. | the aspartic protease gene of a zygomycete fungus mucor pusillus was expressed in saccharomyces cerevisiae under the control of the yeast gal7 promoter. a putative preproenzyme with an nh2-terminal extension of 66 amino acids directed by the gene was processed in yeast cells and the mature enzyme, whose nh2-terminus was identical to that of the mucor enzyme, was efficiently secreted into the medium at a concentration exceeding 150 mg/l. the enzyme secreted from the recombinant yeast was more gly ... | 1987 | 3323844 |
| primary structure of a precursor to the aspartic proteinase from rhizomucor miehei shows that the enzyme is synthesized as a zymogen. | in order to characterize the zymogen of the milk-clotting enzyme from rhizomucor miehei, we constructed a cdna library on pbr327 in escherichia coli. aspartic proteinase-specific recombinants were isolated by colony hybridization to a specific oligonucleotide mixture, and the cdna sequence corresponding to a precursor form of the enzyme was determined. the deduced amino acid sequence shows that this secreted fungal proteinase is synthesized as a precursor. the first 22 amino acid residues in thi ... | 1986 | 3329734 |
| effect of inducers on metabolism of benzo(a)pyrene in vivo and in vitro: analysis by high pressure liquid chromatography. | the characterisation of metabolites formed from benzo(a)pyrene (bp) by aspergillus ochraceus ts and effect of inducers on bp metabolism are reported. the high pressure liquid chromatographic profile of bp metabolites was similar to that of mammalian microsomes furnishing diols, quinones and phenols. the production of bp-4,5-dihydrodiol (k-region diol) by aspergillus ochraceus ts seems to be novel and provides first report on bp metabolism by eukaryotic fungi. in control, phenols and quinones wer ... | 1988 | 3415704 |
| effect of nacl on the accumulation of glycerol by three aspergillus species. | the accumulation of glycerol was investigated in three aspergillus species, a. niger, a. ochraceus and a. tamarii after being grown in media containing different nacl concentrations. intra-extracellular as well as total glycerol were markedly accumulated by the three organisms in response to increased salinity. however, at salinity levels of 10-14% nacl, extracellular glycerol was somewhat lowered. in addition, it was found that the maximum accumulation of glycerol in a. niger and a. tamarii was ... | 1987 | 3449615 |
| cloning and sequencing of a gene for mucor rennin, an aspartate protease from mucor pusillus. | the aspartate protease of mucor pusillus (mucor pusillus rennin; mpr) is a milk-clotting enzyme used in the cheese industry. the partial amino acid sequence of mpr was determined and oligonucleotide probes were synthesized for cloning of the mpr gene. a clone giving positive hybridization with the probes was selected from the cosmid library. sequencing of the cloned dna revealed an open reading frame of 1281 bp without introns which encodes 361 amino acids for the expected mpr with an nh2-termin ... | 1986 | 3534790 |
| microbiological diagnosis of suppurative keratitis in bangladesh. | experience in setting up an inexpensive microbiology laboratory in the bangladesh national society for the blind eye hospital and training complex at chittagong is presented, together with the results of a pilot study to identify organisms responsible in 33 consecutive cases of suppurative keratitis in the chittagong area of bangladesh. of the 33 cases 21 were positively identified by means of gram stain and/or culture. two-thirds of the responsible organisms were bacteria, and one-third were fu ... | 1987 | 3555609 |
| effect of roasting on ochratoxin a level in green coffee beans inoculated with aspergillus ochraceus. | the heat stability of ochratoxin a in green coffee beans inoculated with aspergillus ochraceus was studied. heat treatment (roasting) at 200 degrees c for 10 or 20 min reduced the levels of ochratoxin a by only 0-12% in the dried whole beans. almost all of the ochratoxin a was infused into the coffee decoction when the roasted samples were ground and extracted with boiling water. therefore, the reduction of ochratoxin a concentration of contaminated coffee beans by roasting under these condition ... | 1987 | 3574430 |
| utilization of glucose and phosphate by fungus aspergillus ochraceus during submerged fermentation and its relationship to the production of anti-viral substance 6-mfa. | | 1986 | 3597127 |
| characterization of progesterone 11 alpha-hydroxylase of aspergillus ochraceus ts: a cytochrome p-450 linked monooxygenase. | the monooxygenase of aspergillus ochraceus ts capable of 11 alpha-hydroxylation of progesterone has been resolved into three components and characterized as (i) cytochrome p450, (ii) nadph-cytochrome p450-reductase and (iii) phosphatidyl choline. the 11 alpha-hydroxylase was observed to be nadph dependent, and hydroxylation was enhanced by a nadph regenerating system. this fungal monooxygenase has many features in common with that of mammalian liver microsomes. the role of mammalian cytochrome p ... | 1987 | 3657154 |
| modulation of benzene toxicity by an interferon inducer (6mfa). | repeated intraperitoneal administration of benzene (1.0 ml/kg body wt) for 3 days produced leucopenia, lymphocytopenia and an increased number of nucleated cells in the bone marrow and significantly decreased organ weights of thymus (p less than 0.001) and spleen (p less than 0.001) in female albino rats. iron content, lipid peroxidation and superoxide dismutase activity of the liver and bone marrow were significantly increased as a result of benzene exposure. prior administration of 6mfa, an in ... | 1986 | 3705090 |
| anti-inflammatory activity of 6mfa, a compound obtained from fungus aspergillus ochraceus. | 6mfa, an interferon-inducing substance obtained from fungus, aspergillus ochraceus, has shown anti-inflammatory activity both in acute and chronic animal models of inflammation. it was found that 6mfa was equally effective in inhibiting both exudative as well as granulative phase of inflammation. the compound suppressed also cellular migration during inflammatory process and potentiated significantly the anti-inflammatory activity of indomethacin. the compound was devoid of analgesic or antipyre ... | 1986 | 3784110 |
| postharvest production of ochratoxin a by aspergillus ochraceus and penicillium viridicatum in barley with different protein levels. | the production of ochratoxin a (oa) in barley by aspergillus ochraceus and penicillium viridicatum was measured at 12 and 25 degrees c. the grain had been fertilized with various amounts of nitrogen fertilizer (0, 90, or 240 kg/ha) and contained (at crop maturity) 9.1, 10.4, or 12.0% protein, respectively. the production of oa by both fungi increased as the protein concentration increased. glutamic acid and proline were enriched relative to other amino acids as the protein concentration increase ... | 1985 | 4004212 |
| caffeine degradation and increased ochratoxin a production by toxigenic strains of aspergillus ochraceus isolated from green coffee beans. | the growth and ochratoxin a production of aspergillus ochraceus strains s-235-100 and ifm 0458, which were isolated from green coffee beans and glutinous rice, respectively, were examined in yeast extract-sucrose (yes) medium containing 0.1 to 1.0% caffeine. the mycelial growth and ochratoxin a formation of strain ifm 0458 was inhibited by caffeine at concentrations over 0.1%, and ochratoxin a was not produced at caffeine levels of 0.5% and 1.0%. contrary to this, a. ochraceus strain s-235-100 p ... | 1985 | 4033738 |
| [effect of ochratoxin a and other intracellular metabolites on the phosphogluconate metabolic pathway in aspergillus ochraceus]. | | 1985 | 4036670 |
| effects of cysteine and structurally related compounds on ochratoxin production by aspergillus ochraceus. | addition of 10(-2) m l-cysteine, l-cystine, or s-ethyl-l-cysteine to a synthetic medium containing xylose as the sole carbon source did not decrease ochratoxin production by aspergillus ochraceus. at that concentration, dl-homocysteine thiolactone hc1, dl-cysteine hc1, l-ethionine, s-methyl-l-cysteine, and glutathione (reduced) strongly inhibited ochratoxin production. dl-homocysteine thiolactone hc1, dl-cysteine hc1, and l-ethionine also strongly inhibited fungal growth. at lower concentrations ... | 1985 | 4092177 |
| [isolation of a fibrinolytically active protease from aspergillus ochraceus]. | | 1971 | 4106006 |
| [effect of a protease from aspergillus ochraceus on platelet function]. | | 1971 | 4106008 |
| [microbiological isolation of a protease with fibrinolytic effect from aspergillus ochraceus]. | | 1974 | 4134259 |
| [animal experimental studies on a protease from aspergillus ochraceus]. | | 1974 | 4134260 |
| [clinical and coagulation physiological studies on a protease from aspergillus ochraceus (preliminary report)]. | | 1974 | 4134261 |
| [characterization of an alkaline protease form aspergillus ochraceus]. | | 1974 | 4134483 |
| studies on the proteolysis of fibrinogen and fibrin by aspergillus ochraceus enzyme as compared to the action of plasmin. | | 1974 | 4134484 |
| medium-scale production and purification of ochratoxin a, a metabolite of aspergillus ochraceus. | the preparation of crystalline ochratoxin a from aspergillus ochraceus nutrient solution is described. methods are adaptable to large-scale fermentations. | 1972 | 4336018 |
| fibrinolysis by protease of aspergillus ochraceus. | | 1972 | 4669810 |
| influence of nitrogen source on the synthesis of fat from sucrose by aspergillus terreus, aspergillus ochraceus, cladosporium fulvum, cladosporium herbarum and penicillium gladioli. | | 1972 | 4672593 |
| [aspergillus ochraceus protease: an additional enzyme for the determination of incomplete erythrocyte antibodies]. | | 1972 | 4675273 |
| microbiological hydroxylation of steroids. vi. hydroxylation of simple mono- and di-oxygenated 5 -androstanes and of 3-oxoestranes with the fungus aspergillus ochraceus. | | 1972 | 4675916 |
| effect of zinc, copper, and iron on ochratoxin a production. | the effect of zinc, copper, and iron levels on production of ochratoxin a by aspergillus ochraceus wilhelm in a synthetic medium in a shake culture was investigated. optimal concentrations of znso(4), cuso(4), and fecl(3) for ochratoxin a production were 0.055 to 2.2 mg/liter, 0.004 to 0.04 mg/liter, and 1.2 to 24 mg/liter, respectively. zinc and copper levels greater than optimum reduced the rate of ochratoxin accumulation without altering either glutamate or sucrose utilization. ochratoxin a p ... | 1973 | 4715563 |
| production of ochratoxins a and b on country cured ham. | two strains of aspergillus ochraceus and six of penicillium viridicatum isolated from country cured hams were screened for production of ochratoxins a and b. none of the isolated p. viridicatum strains yielded detectable amounts of ochratoxin a or b, whereas both strains of a. ochraceus produced ochratoxins a and b on rice, defatted peanut meal, and country cured ham. after 21 days of incubation on ham, one-third of the toxin was found in the mycelial mat on the ham surface, whereas two-thirds h ... | 1973 | 4737852 |
| effect of time and temperature on ochratoxin a production by aspergillus ochraceus. | | 1973 | 4762800 |
| the component fatty acids of aspergillus ochraceus fat. | | 1973 | 4764005 |
| comparative toxicity of ochratoxin a and crude aspergillus ochraceus culture extract in japanese quail (coturnix coturnix japonica). | | 1973 | 4789009 |
| production of penicillic acid and ochratoxin a on poultry feed by aspergillus ochraceus: temperature and moisture requirements. | a strain of aspergillus ochraceus wilhelm, isolated from poultry feed, produced both penicillic acid and ochratoxin a. studies demonstrating the ability of this fungus to colonize poultry feed and produce these two mycotoxins under various temperatures and moistures indicated that the interaction was complex. the optimal temperature for conidial development did not vary with moisture, but accumulation of both toxins did. a combination of low temperature, 15 or 22 c, and low moisture favored the ... | 1973 | 4795527 |
| efects of dichlorvos on ochratoxin production by aspergillus ochraceus. | | 1974 | 4840523 |
| production of 3-(1,2-epoxypropyl)-5,6-dihydro-5-hydroxy-6-methylpyran-2-one by aspergillus ochraceus wilhelm. | | 1974 | 4840919 |