| cell-mediated immune responses in mice infected with fonsecaea pedrosoi. | time course of cellular and humoral immune responses in mice infected with fonsecaea pedrosoi was investigated by using an antigen prepared from culture filtrate of this fungus. mice were infected by intravenous injection with yeast-like cells of the fungus. viable fungus was recovered from the brain of the infected mice until the 36th day after inoculation, and from the other organs examined until 14th to 16th day. inflammatory lesions were observed in the brain, lung, heart, liver, spleen, kid ... | 1979 | 386129 |
| chromomycosis. successful treatment with 5-fluorocytosine. | a case of chromomycosis, caused by fonsecaea pedrosoi, was treated with 5-fluorocytosine. after 18 weeks of treatment the initial lesions had largely resolved, and no evidence of active disease was observed clinically, histologically or from fungal cultures of the biopsy material. further resolution of the remaining hypertrophic scar was achieved by intralesional injections of triamcinolone acetonide. minimal side-effects were encountered during the therapy, and no recurrence during an 18-month ... | 1979 | 438397 |
| [dermatology in french guiana]. | in french guayana, climatic and geographic conditions enhance development of numerous fungal, parasitic and microbial skin diseases. the most particular infectious diseases of this french department are lobomycosis, chromomycosis (phialophora pedrosoi), creeping disease, cutaneous leishmaniasis, tungiasis, mycobacterial ulcus (mycobacterium ulcerans) and papular dermatitis caused by nettling hairs of lepidoptera (hylesia urticans). the occurence of these skin diseases is demonstrative of patholo ... | 1979 | 511550 |
| [synergistic action of amphotericin b and 5-fluorocytosine on fonsecaea pedrosoi]. | | 1979 | 550288 |
| [susceptibility tests of fonsecaea pedrosoi strains to the combination of thiabendazole and amphotericin b "in vitro"]. | | 1979 | 550289 |
| skin test-active substance prepared from culture filtrate of fonsecaea pedrosoi. | ethanol-precipitated substance (ep) was prepared from culture filtrate of fonsecaea pedrosoi. ep was separated into two components by passing through a sephadex g-50 column; the faster passing component was referred to as ep-1, the slower as ep-2. ep-1 and ep-2 were evaluated as an antigen for detecting cutaneous delayed hypersensitivity in patients with chromomycosis. ep-1 elicited positive delayed skin reactions in all of 8 patients with chromomycosis, of which 7 caused by f. pedrosoi and one ... | 1979 | 573371 |
| [tropic-subtropic fungus infections in germany]. | observations by the author and other workers on exotic mycoses in germany are used in order to establish several groups of patients. the following mycoses from tropical and subtropical areas and their causative agents are mentioned: tinea nigra (cladosporium werneckii), south american blastomycosis (paracoccidioides brasiliensis), coccidioidomycosis (coccidioides immitis), histoplasmosis (histoplasma capsulatum), mycetoma (madurella mycetomi), dermatophytosis/tinea capitis (trichophyton soudanen ... | 1978 | 627480 |
| serologic cross-reactivity of the d-galacto-d-mannans isolated from several pathogenic fungi against anti-hormodendrum pedrosoi serum. | | 1975 | 804991 |
| effect of carbon source on conidiogenesis in fonsecaea dermatitidis, agent of chromomycosis. | hormiscium dermatitidis kano is a well known etiological agent of cutaneous and generalized chromomycosis. however, the generic designation of this fungus has long been a much debated question. the results of the present study of the type culture atcc 28869 indicate that the fungus is polymorphic, producing a phialophora state in media containing glucose or maltose and a cladosporium state in media containing galactose or melibiose. morphologically and developmentally this chromomycotic agent is ... | 1976 | 945462 |
| [chromomycosis in iberia]. | a 66 year old, spaniard, born at sueca (valencia province), was found infected with "fonsecaea pedrosoi". his lesion, located in the inner aspect of the right arm, appeared when he was 7-8 years old, and progressed very slowly until he migrated to venezuela, at 42. thereafter it progressed more rapidly and started to hurt. treatment included administration of 5-flucytosine at the daily dose of 150 mg./kg. during 4 and a half months and thermotherapy by means of a permanent adhesive tape on the l ... | 1976 | 988455 |
| heat-shock response in fonsecaea pedrosoi, a pathogenic fungus. | using two-dimensional electrophoresis we have investigated the heat-shock response in a pathogenic fungus, fonsecaea pedrosoi. fungal cultures were transferred from 37 to 45 degrees c for either 30 or 90 min and then returned back to the initial temperature. analysis of the total proteins resolved on two-dimensional gels indicated important changes in the accumulation of several peptides according to the duration of treatment and the temperature. the 30-min incubation at 45 degrees c resulted in ... | 1992 | 1288846 |
| four non-endemic new zealand cases of chromoblastomycosis. | the majority of cases of chromoblastomycosis are reported from tropical to subtropical countries; only one previous case being reported from new zealand. four non-endemic cases in pacific island patients are described. all of the new zealand cases were caused by fonsecaea pedrosoi. in the present report, one patient was successfully treated by excision of the lesion followed by skin grafting. another was treated with 200 mg ketoconazole daily for 10 weeks with no obvious improvement. no follow-u ... | 1992 | 1303079 |
| [fonsecaea pedrosoi-caused chromoblastomycosis in the state of maranhão. i. the clinical, epidemiological and evolutionary aspects]. | the aim of this study was to observe the clinical and of epidemiological behavior of chromoblastomycosis in the state of maranhão. a retrospective and prospective study of 13 cases was performed at the infectious diseases section of the hospital dos servidores do estado do maranhão, from november, 1988 to july, 1991. in the investigation a protocol record was used with a view to further analysis. a higher prevalence between 50 and 60 years of age (46.1%) and in male patients (92.3%) was noted. t ... | 1992 | 1308064 |
| tropical mycoses. | the most common tropical subcutaneous and deep mycoses include chromomycosis, sporotrichosis and mycetoma. all are commonly found in natal and in other subtropical countries. although blastomycosis is endemic in north america, only 4 cases have been identified in natal during the last 25 years, and all presented with atypical clinical features. african histoplasmosis, caused by histoplasma capsulatum var. duboisii and limited mainly to central and western africa, has been found in only 1 patient ... | 1992 | 1319312 |
| itraconazole in the treatment of chromoblastomycosis due to fonsecaea pedrosoi. | the efficacy and tolerability of itraconazole in chromoblastomycosis due to fonsecaea pedrosoi were evaluated in a non-comparative open clinical trial in 19 brazilian patients with histopathologically and mycologically proven active chromoblastomycosis. patients were classified in terms of severity and received itraconazole at the dosage of 200 to 400 mg per day until previously described criteria of cure have been reached. clinical, mycologic, histopathologic, and laboratory evaluations were pe ... | 1992 | 1330949 |
| fonsecaea pedrosoi: lipid composition and determination of susceptibility to amphotericin b. | conidia and mycelial cells of fonsecaea pedrosoi atcc 46428 were obtained for analyses of lipid composition. total lipids, phospholipids, sterols, and qualitative sterols and fatty acid composition were determined. a higher lipid content was detected in conidia than in mycelial cells of fonsecaea pedrosoi, which could not be attributed to total sterols and phospholipids. in both forms of this fungus, ergosterol was the only sterol detected. the minimal inhibitory concentration of amphotericin b ... | 1992 | 1393822 |
| effect of environmental factors on fonsecaea pedrosoi morphogenesis with emphasis on sclerotic cells induced by propranolol. | the influence of growth conditions, as well as of propranolol on fonsecaea pedrosoi morphogenesis was established using the chemically defined media of czapeck-dox (cd) and butterfield (bf). mycelial growth of f. pedrosoi in both media was obtained at room temperature (25 degrees c) for 14 days, without shaking, whereas conidia formed at 37 degrees c, for 4 days, in shaken cultures and could be isolated free from the mycelium by filtration in gauze. at low ph (2.5-3.0), there appeared sclerotic ... | 1992 | 1406903 |
| inhibitory effect of melanin on the interaction of fonsecaea pedrosoi with mammalian cells in vitro. | conidial forms of fonsecaea pedrosoi, grown under conditions where melanin was or was not synthesized, were allowed to interact with normal and cytochalasin treated macrophages. melanin-free conidia were more infective to the macrophages. treatment of macrophages with either cytochalasin b or d before the interaction decreased, but did not totally prevent their infection by the fungi. this inhibitory effect was higher (approximately 90%) if f. pedrosoi was grown under conditions where melanin wa ... | 1992 | 1432486 |
| [morphologic and physiologic characteristics of costa rica pathogenic fungi (dermatiaceae)]. | forty one strains of dematiaceous fungi from the mycology collection of the university of costa rica were studied. thirty three were pathogenic (fonsecaea pedrosoi, cladosporium carrionii, xylohypha bantiana, exophiala jeanselmei, rhinocladiella aquaspersa, phialophora verrucosa) and the other eight were contaminants (hormodendrum sp.). morphological studies were done using the slide culture technique. the physiological criteria used were: urease production, gelatin and loeffler media liquefacti ... | 1991 | 1844148 |
| characterization of fonsecaea pedrosoi melanin. | the constituents of the melanin complex from mycelial forms of fonsecaea pedrosoi were partially characterized. the pigment was mainly accumulated on large alkali-extractable, electron-dense cytoplasmic bodies (melanosomes) and, apparently, on the outer layer of the cell wall as external deposits within verrucose outgrowths. using electron microscopy and thiéry's periodate/thiosemicarbazide/silver proteinate staining method, glycogen-like particles were also detected at the periphery of the cell ... | 1991 | 1856679 |
| treatment of chronic murine chromoblastomycosis with the triazole sch39304. | congenitally athymic (nu/nu) mice were inoculated sc with 10(5) conidia of fonsecaea pedrosoi and treated orally from the 1st to the 16th week of infection with either a new triazole, sch39304, or itraconazole at doses of 20 or 60 mg/kg/day. the volumes of the lesions were measured with calipers at 4 week intervals and compared statistically by the wilcoxon test. at the end of the experiment, mice were killed and samples of the lesions were examined histopathologically and by electron microscopy ... | 1990 | 2164791 |
| fine structure and cytochemistry of the interaction between fonsecaea pedrosoi and mouse resident macrophages. | the interaction between conidia of fonsecaea pedrosoi and mouse resident peritoneal macrophages was observed by light microscopy and by scanning (sem) and transmission (tem) electron microscopy. the conidia first attached to the surface of the macrophage and were then ingested. prolonged incubation of the macrophage cultures showed proliferation of intracellular fungi as well as those which remained attached to the macrophage surface. the conidia were ingested by a typical phagocytic process, wi ... | 1990 | 2283584 |
| chromoblastomycosis. | chromoblastomycosis, together with phaeohyphomycosis and mycetoma, makes up the disease entities caused by the dematiaceous fungi. most cases of chromoblastomycosis are caused by five genera of fungi: fonsecaea compactum, fonsecaea pedrosoi, phialophora verrucosa, cladosporium carrionii, and rhino-cladiella aquaspersa. the disease has a cosmopolitan distribution but predominates in rural, agricultural settings. clinically, chromoblastomycosis is hallmarked by verrucous nodules at the site of fun ... | 1989 | 2670367 |
| physiological characteristics of environmental isolates of pathogenic dematiaceous fungi. | a total of 39 environmental isolates of pathogenic dematiaceous fungi (fonsecaea pedrosoi: 14 isolates, phialophora verrucosa: 6, cladosporium carrionii: 9, exophiala jeanselmei: 2, ramichloridium subulatum: 6, cladosporium tenuissimum: 1 and phaeoisaria clematidis: 1) were evaluated for their various physiological characteristics including the ability to produce extracellular enzymes. significant physiological characteristics included the ability of isolates of all the species except c. tenuiss ... | 1989 | 2710157 |
| lipases of fonsecaea pedrosoi and phialophora verrucosa. | lipase activity was demonstrable titrimetrically in the culture filtrates of fonsecaea pedrosoi and phialophora verrucosa on the 6th day of incubation reaching a peak on the 15th and 12th days respectively for the two fungi. purified lipases of f. pedrosoi and p. verrucosa, with specific activities of 36.0 and 39.4 fold increase respectively, were obtained by cold acetone extraction, gel filtration followed by ion exchange chromatography. the lipases had the same optimum ph (5.5) and temperature ... | 1989 | 2729958 |
| experimental murine chromomycosis mimicking chronic progressive human disease. | congenitally athymic (nu/nu) mice, mice defective in nk cell and macrophage function (bg/bg), and normal balb/c mice were inoculated sc with 10 conidia of fonsecaea pedrosoi (fp). in immunologically intact and immunodeficient mice, a local infection developed approximately 2 weeks post-inoculation and enlarged over 1-2 weeks. in bg/bg and normal nu/+ mice, lesions resolved within 5-6 weeks. however, nu/nu mice continued to have enlarging sc lesions during greater than 4-6 months of observation. ... | 1989 | 2742041 |
| a clinical trial of itraconazole in the treatment of deep mycoses and leishmaniasis. | itraconazole was administered orally to two patients with sporotrichosis, 10 patients with paracoccidioidomycosis, three with mycetomas (due to madurella grisea, streptomyces madurae, and pseudochaetosphaeronema larense, respectively), nine with chromomycosis due to cladosporium carrionii, five with chromomycosis due to fonsecaea pedrosoi and five with leishmaniasis (including one with the nodular disseminated form). the clinical and laboratory tests showed excellent tolerance to the drug with a ... | 1987 | 3027848 |
| itraconazole for deep mycoses: preliminary experience in mexico. | itraconazole has been used as oral therapy for deep mycoses since july 1984 in our institution. so far, therapy has been evaluated for eight patients: one with cutaneous coccidioidomycosis; two with chromomycosis (fonsecaea pedrosoi); and five with sporotrichosis (three fixed, one lymphangitic, and one disseminated). clinical and mycologic evaluations were done at the beginning of treatment, 15 days after the initiation of treatment, and monthly thereafter. dosage was 100-200 mg per day, and dur ... | 1987 | 3027849 |
| exoantigen test for cladosporium bantianum, fonsecaea pedrosoi, and phialophora verrucosa. | exoantigens from 10-day-old cultures of 100 isolates of pathogenic and saprophytic dematiaceous fungi were analyzed by the exoantigen test. antisera to cladosporium bantianum atcc 10958, fonsecaea pedrosoi cdc amo-b06, and phialophora verrucosa cdc amo-c12 were prepared in new zealand rabbits immunized with soluble antigens from 1-month-old cultures. absorbed and nonabsorbed antisera and exoantigens from the same organisms were used as reference reagents. serologic reactions were analyzed in ter ... | 1986 | 3084549 |
| cerebral phaeohyphomycosis caused by fonsecaea pedrosoi in saudi arabia. | a case of cerebral phaeohyphomycosis (cpm) in a 70-yr-old saudi male was diagnosed recently at king khalid university hospital in riyadh. computerized tomography (ct) scans of the patient's brain unveiled 2 abscesses in the left frontal and a 3rd abscess in the right frontal lobes. aspirated pus from the abscesses contained branched, septate, brown hyphae diagnostic of cpm. culturing of pus yielded a slow-growing, dematiaceous fungus which was identified as fonsecaea pedrosoi. combined therapy o ... | 1988 | 3179083 |
| multiseptate bodies in systemic phaeohyphomycosis diagnosed by fine needle aspiration cytology. | fine needle aspiration of a lung nodule in an immunosuppressed adult man revealed numerous dematiaceous (pigmented) fungi with both hyphal and spherical forms. multiseptate bodies ("muriform" cells) were also present in small numbers. fonsecaea pedrosoi was cultured from the aspirate. the cytomorphologic features of the fungus in tissue are described, and the significance of finding fungal cells that divide both by budding and fission is discussed. | 1988 | 3201878 |
| evaluation of proteolytic activity to differentiate some dematiaceous fungi. | a total of 123 isolates of cladosporium spp., exophiala spp., fonsecaea spp., lecythophora hoffmannii, phaeoannellomyces werneckii, phialophora spp., wangiella dermatitidis, and xylohypha bantiana were tested for proteolytic activity by using 26 different formulations of gelatin, milk, casein, and loeffler media. other physiological properties examined included hydrolysis of tyrosine and xanthine, sodium nitrate utilization in czapek dox agar, and thermotolerance. isolates of exophiala jeanselme ... | 1988 | 3343325 |
| immunochemical characterisation of antigens and growth inhibition of fonsecaea pedrosoi by species-specific igg. | antigens of fonsecaea pedrosoi, the most common agent of chromomycosis, were characterised by immunoprecipitation with a rabbit antiserum raised against the cell-protein extract and serum from an infected patient. thirteen antigens were commonly detected and, as some of these antigens could be iodinated, they may be present in the fungal cell wall. purified igg from the rabbit antiserum was shown to produce a 50-60% inhibition of fungal growth. some of the antigens characterised may be important ... | 1988 | 3392728 |
| comparative histopathology of dactylaria constricta, fonsecaea pedrosoi, wangiella dermatitidis, and xylohypha bantiana in experimental phaeohyphomycosis of the central nervous system. | | 1987 | 3614262 |
| in vitro sensitivity of environmental isolates of pathogenic dematiaceous fungi to azole compounds and a phenylpropyl-morpholine derivative. | the in vitro sensitivity (minimum inhibitory concentrations; mics) of 42 environmental isolates of pathogenic dematiaceous fungi to 7 azole compounds, viz. thiabendazole, ketoconazole, miconazole, econazole bifonazole, bay n 7133, bay 1 9139 and phenylpropyl-morpholine derivative, ro14-4767/002 was studied by an agar dilution method using emmon's sabouraud dextrose agar (esda) as the culture medium. the isolates of fonsecaea pedrosoi, cladosporium carrionii, exophiala jeanselmei and ramichloridi ... | 1987 | 3657909 |
| use of carbohydrate and nitrate assimilations in the identification of dematiaceous fungi. | a total of 61 isolates of dematiaceous fungi, including exophiala jeanselmei, wangiella dermatitidis, fonsecaea pedrosoi, phialophora verrucosa, and a few isolates of related organisms were evaluated for their ability to assimilate 13 carbohydrates and sodium nitrate. results indicated that patterns of assimilations can facilitate specific identifications when used with microscopic morphologic features. eleven isolates of w. dermatitidis demonstrated negative results for nitrate assimilation, al ... | 1986 | 3709095 |
| identification of sialic acids on the cell surface of hyphae and conidia of the human pathogen fonsecaea pedrosoi. | sialic acids were characterized on the cell surface of conidia and hyphae of fonsecaea pedrosoi, one of the agents of chromoblastomycosis. neuraminidase-treated conidia had a reduced negative electrophoretic mobility and, in comparison with untreated cells, bound fewer particles of colloidal iron hydroxide and of cationized ferritin. sialic acid residues in conidia are linked to galactopyranosyl units as indicated by the increased reactivity of neuraminidase-treated cells with peanut agglutinin. ... | 1986 | 3723289 |
| studies on pathogenic dematiaceous fungi. 1. isolation from natural sources. | two hundred and twenty-six samples of woody materials, vegetable matter and soil were processed by the direct plating and mouse inoculation technique for the isolation of pathogenic dematiaceous fungi. the species of fungi isolated were fonsecaea pedrosoi - 13, cladosporium carrionii - 7 and phialophora verrucosa -4 isolates. the mouse inoculation technique was found to be much better than direct plating for the recovery of these fungi. woody plant materials proved to be a good sample source for ... | 1986 | 3724831 |
| [an unusual localization of chromoblastomycosis]. | most african cases of chromoblastomycosis present with lesions on the arms or legs. we report the case of a 48 years old man, a retired professional hunter, living in the haut-ogooué province of gabon. the patient presented with extensive lesions of the upper thorax and left shoulder but none on the limbs. direct examination of crusts and skin biopsies revealed spherical, septated, pigmented bodies. fonsecaea pedrosoï was isolated in culture. the patient was treated with ketoconazole (400 mg per ... | 1986 | 3773687 |
| studies on pathogenic dematiaceous fungi, ii. pathogenicity of fonsecaea pedrosoi and phialophora verrucosa for laboratory mice. | | 1986 | 3796678 |
| granulomatous reactions induced by lipids extracted from fonsecaea pedrosoi, fonsecaea compactum, cladosporium carrionii and phialophora verrucosum. | granulomatous reactions induced by lipid extracts from the dermatophyte fungi fonsecaea pedrosoi, fonsecaea compactum, cladosporium carrionii and phialophora verrucosum, the causal organisms of chromoblastomycosis, were studied. charcoal particles coated with the lipid extracts were prepared and injected intravenously into mice. inflammation was characterized by an intense mononuclear cell infiltrate that lodged in the lung from 4 to 8 d after inoculation. | 1985 | 3989508 |
| [treatment of chromomycosis with a combination of 5-fluorocytosine and thiabendazole. apropos of a zairian case due to fonsecaea pedrosoi]. | | 1985 | 4004379 |
| comparative electrophoresis, isoelectric focusing and numerical taxonomy of some isolates of fonsecaea pedrosoi and allied fungi. | the proteins in broken-cell extracts from eight isolates of fonsecaea pedrosoi, the principal agent of chromomycosis, were studied and compared by electrophoresis and isoelectric focusing. a type pattern was established with 16 fractions ranging in molecular weight between 7600 and 78500 daltons and 16 fractions varying in isoelectric point between 4.95 and 7.90. a genetic distance of 0.1 found by the numerical study applied to both analyses reveals a considerable similarity among the isolates s ... | 1985 | 4049186 |
| role of the fungal cell wall in the granulomatous response of mice to the agents of chromomycosis. | mice were given, intraperitoneally, inocula of a cell-wall preparation and fractions thereof from fonsecaea pedrosoi, f. compactum, cladosporium carrioni and phialophora verrucosum. large doses of cell-wall preparation, with or without trypsin treatment, produced a pronounced loss of body weight, a granulomatous reaction and, sometimes, death. after extraction of the cell wall preparation with 1n naoh, three fractions were obtained: an alkali-insoluble fraction 1; an alkali-soluble acid-insolubl ... | 1985 | 4068024 |
| septal ultrastructure in phialophora pedrosoi, phialophora verrucosa and cladosporium carrionii. | | 1973 | 4127193 |
| spontaneous occurrence and experimental transmission of the fungus, fonsecaea pedrosoi, in the marine toad, bufo marinus. | | 1973 | 4347757 |
| the frequent isolation of phialophora verrucosa and phialophora pedrosoi from natural sources. | | 1972 | 4640043 |
| [association of chromoblastomycosis (fonsecaea pedrosoi) and histoplasmosis (histoplasma capsulatum) in south cameroon]. | | 1973 | 4801858 |
| a serological comparison of phialophora verrucosa, fonsecaea pedrosoi and cladosporium carrionii using immunodiffusion and immunoelectrophoresis. | | 1970 | 4993154 |
| pathogenicity of phialophora pedrosoi. | | 1971 | 5540278 |
| [habitat of nocardia asteroides, phialophora pedrosoi and cryptococcus neoformans in venezuela]. | | 1968 | 5662486 |
| taxonomic criteria for the fungi of chromoblastomycosis with reference to fonsecaea pedrosoi. | | 1971 | 5900177 |
| antimycotic therapy of experimental infections caused by dematiaceous fungi. | experimental infections of mice with wangiella dermatitidis and fonsecaea pedrosoi provided a model for evaluating new antifungal agents or new combination therapy. in our models flucytosine exerted a dose-related therapeutic effect on the acute and on the more chronic infection. in the acute wangiella infection amphotericin b also showed therapeutic activity whereas in the fonsecaea model the effect was weak. the azole derivative ici 153066 was the most efficacious drug in the wangiella model w ... | 1984 | 6095469 |
| serological cross-reactivity between sporothrix schenckii and various unrelated fungi. | the serological cross-reactivity of sporothrix schenckii with various unrelated fungi was investigated by use of immunodiffusion tests. a rabbit anti s. schenckii serum was obtained, which reacted with cladosporium werneckii, c. carrionii, c. bantianum, coccidioides immitis, phialophora jeanselmei, p. gougerotii, p. dermatitidis, fonsecaea pedrosoi, aspergillus fumigatus, histoplasma capsulatum and trichophyton mentagrophytes, but not with saccharomyces cerevisiae antigens. the serological deter ... | 1981 | 6163987 |
| chronic mucocutaneous candidosis and other superficial and systemic mycoses successfully treated with ketoconazole. | four patients with chronic mucocutaneous candidosis from early infancy were treated successfully with ketoconazole given orally. all thrush lesions were clinically and mycologically cured within a few days of treatment with 100-400 micrograms of ketoconazole daily; skin lesions were cured within a few weeks, and nails were cured after about three months of treatment. delayed cutaneous hypersensitivity to candidin was acquired by the third month. cellular and humoral immunologic responses were re ... | 1980 | 6255540 |
| treatment of chromomycosis with ketoconazole and 5-fluorocytosine. | chromomycosis is a disfiguring cutaneous mycosis that is often recalcitrant to therapy. we describe a case of chromomycosis of the lower extremities that is being successfully treated with ketoconazole and 5-fluorocytosine (5-fc) after treatment with ketoconazole alone had failed. in vitro studies showed that the drugs acted synergistically against the strain of fonsecaea pedrosoi which was isolated from the patient's lesion. | 1983 | 6298285 |
| [chromomycosis of the buttocks]. | we report a case of chromomycosis involving buttocks in a 63 years old white male patient, who was living temporaly in several tropical areas of argentine, due to professional reasons. the lesion had arisen 28 years ago, located in his right buttock. clinical examination showed a large, erythematous and violaceus plaque, with atrophic center and peripheric warty nodules. diagnosis was established on histologic and mycologic examination. fonsecaea pedrosoi was isolated as the ethiologic agent. tr ... | 1984 | 6396470 |
| activation of the alternative complement pathway by fonsecaea pedrosoi. | human sera were examined for evidence of complement activation by fonsecaea pedrosoi. the serum that had been incubated with f. pedrosoi showed immunoelectrophoretic c3 conversion and generation of c5a, as measured by radioimmunoassay. c3 conversion and c5a generation did not occur in serum chelated with edta, or in serum heated to 56 degrees c for 30 min. serum depleted of the heat (50 degrees c x 30 min)-labile factor b was also deficient in c3 conversion and c5a generation. complement activat ... | 1984 | 6481182 |
| study of the conidial development and cleistothecium-like structure of some strains of fonsecaea pedrosoi. comparison with other close dematiaeae. | the production of twenty-seven strains of fonsecaea pedrosoi was studied. the denticulate type (asexual reproduction) showed three morphological variations: medium-size, long and sessile forms. these forms can be used to characterize the different strains isolated especially for epidemiological purposes. only one strain (mr 1335) isolated from a chromomycosis case in martinique showed a cleistothecium-like structure. this formation was compared to that observed in phialophora verrucosa, another ... | 1984 | 6538933 |
| experimental infection of mice by fonsecaea pedrosoi and wangiella dermatitidis. | after i.v. inoculation in cortisone-pretreated mice fonsecaea pedrosoi produced a chronic infection characterized by black lesions in the skin and subcutis with sclerotic bodies resembling the tissue form of human chromomycosis in addition to lesions in the brain and other organs. wangiella dermatitidis inoculated into unpretreated mice produced an acute, fatal cerebral infection closely resembling human phaeohyphomycosis. abundant hyphal growth was observed in the brain tissue. | 1984 | 6539507 |
| successful treatment of chromoblastomycosis with topical heat therapy. | four female patients with chromoblastomycosis were completely cured by prolonged topical application of tolerable heat from pocket warmers. the lesions involuted after 2, 3, and 6 months, respectively, in three patients who faithfully followed our strict treatment schedule, in contrast to the fourth patient who performed the topical heat therapy in an irregular manner at home over a 12-month period. in vitro studies showed that the mature colonies of fonsecaea pedrosoi, isolated from three of th ... | 1984 | 6715610 |
| auricular chromomycosis. | chromomycosis, a relatively uncommon infection caused by a variety of dematiaceous fungi, eg, phialophora verrucosa, fonsecaea pedrosoi, fonsecaea compacta, cladosporium carrionii, rhinocladiella aquaspersa, and cladophialophora ajelloi, reflects a spectrum of disease ranging from an usually localized cutaneous form to rare visceral involvement. the cutaneous form has been reported to involve almost any site on the body surface. however, the lesions are usually confined to the extremities, face, ... | 1983 | 6849572 |
| scanning electron microscopic observation of the parasitic forms of fonsecaea pedrosoi in a human skin lesion. | the parasitic form of fonsecaea pedrosoi in the superficial hyperkeratotic layer of the skin, in a patient with chromoblastomycosis, was observed by a scanning electron microscope using some technical improvements. the fungus elements were comprised of branched septate hyphae with spherical cells and sclerotic cells. it was observed that the sclerotic cells, divided into two or three parts, formed a germ tube. | 1983 | 6877340 |
| evaluation of skin test for chromoblastomycosis using antigens prepared from culture filtrates of fonsecaea pedrosoi, phialophora verrucosa, wangiella dermatitidis and exophiala jeanselmei. | antigenic substances were prepared from culture filtrates of fonsecaea pedrosoi, phialophora verrucosa, wangiella dermatitidis and exophiala jeanselmei. these antigenic substances were evaluated for detecting cutaneous delayed hypersensitivity in rats experimentally-infected with f. pedrosoi, p. verrucosa. w. dermatitidis, e. jeanselmei, cladosporium carrionii and fonsecaea compactum and in patients with chromoblastomycosis caused by f. pedrosoi. the f. pedrosoi antigen elicited positive reactio ... | 1982 | 7070478 |
| [five cases of chromomycosis in gabon (author's transl)]. | five cases of chromomycosis were studied in the parasitological and mycological department of the national laboratory and c.u.s.s. in libreville (gabon). different types of lesions were observed which were more often maculous than verrucous. in one case, it was localized on the upper limb, and in four cases on the lower limbs. they all were diagnosed after a 2 to 25 years evolution. fonsecaea pedrosoï was isolated in every case. direct examinations always recovered the classical scleratic cells ... | 1982 | 7078383 |
| isolation of dematiaceous pathogenic fungi from a feed and seed warehouse. | in an epidemiological study, nine isolates of dematiaceous fungi were recovered from the interior of a local feed and seed warehouse. sample sites include brick walls and floors. air samples also were included. samples were collected in saline and plated on mycobiotic and sabouraud agar. the nine dematiaceous fungi recovered from these samples were identified with microscopic morphology, thermotolerance, biochemical reactions, and animal virulence test. four isolates were identified as nonpathog ... | 1982 | 7200100 |
| observations on the chemical compositions of some pathogenic fungi for humans determined by x-ray microanalysis. | the electron microprobe analyzer was used to study the chemical compositions of some pathogenic fungi for humans. elementary analysis was based on the characteristic x-ray spectra generated by the electrons passing through the specimens. this is a non-destructive method allowing to perform multiple elementary analysis in the same tissue. in order to increase our understanding of pathogenic fungi, small pieces of fungi tissue were analyzed. the species examined were histoplasma capsulatum, blasto ... | 1980 | 7203265 |
| fonsecaea pedrosoi isolated from skin crusts of bowen's disease. | fonsecaea pedrosoi was isolated from scaly crusts of plaque lesions on the left thigh of a 60-year-old japanese man. histological examination confirmed these lesions to be due to bowen's disease, and sclerotic cells were recognized in the stratum corneum. to our knowledge, this is the first study to demonstrate superficial colonization of a pre-existing skin lesion by f. pedrosoi. | 1995 | 7477088 |
| [chromoblastomycosis in gabon. study of 64 cases]. | during the last 25 years, 64 cases of chromoblastomycosis were diagnosed in gabon. they came from the entire territory, where living in rural zones men as well as women were affected. the evolution of the disease has lasted for more than 10 years for the majority of the cases. the preferential location is the lower limb for 78.5% of the patients, with a double localization for one case and an exceptional malignant transformation for another case. two clinical aspects emerged, despite an apparent ... | 1995 | 7582644 |
| [the coconut babaçu (orbignya phalerata martins) as a probable risk of human infection by the agent of chromoblastomycosis in the state of maranhão, brazil]. | during a survey of 30 patients with chromoblastomycosis followed at the hospital dos servidores do estado do maranhão, the authors observed in 2 (6.6%) patients with lesions on the buttock. this is an uncommon site for the initial lesions of chromoblastomycosis. there is often a history of microtraumatism during the farming job hence the more frequent development of lesions in the lower limbs. both patients had 10 years disease, with the presence of nodules and vegetant warty lesions in coalesce ... | 1995 | 7724868 |
| [auricular chromoblastomycosis. a case report]. | it is presented a case of auricular chromoblastomycosis mimicking an eczematous lesions. the authors refer the rarity of this localization. all reported cases of auricular chromoblastomycosis have been caused by fonsecaea pedrosoi but in the present case the etiologic agent was phialophora verrucosa. | 1994 | 7732272 |
| primary mycotic abscess of the brain caused by fonsecaea pedrosoi. case report. | a case of cerebral abscess caused by fonsecaea pedrosoi is reported in a nonimmunocompromised 15-year-old boy. in the absence of a primary cutaneous lesion, this fungus was considered to be a primary neuropathogen. the fungus was cultured and characterized from the abscess material obtained at surgery. prompt therapy using amphotericin b and 5-flucytosine helped in the successful recovery of this patient. | 1995 | 7815117 |
| concurrent chromoblastomycosis caused by fonsecaea pedrosoi and actinomycetoma caused by nocardia brasiliensis. | chromoblastomycosis and mycetoma are chronic infections caused by the repeated traumatic inoculation of organisms into the skin and subcutaneous tissue. chromoblastomycosis is caused by several dematiaceous (pigmented) fungi, occurs in tropical regions, and usually affects the lower extremities. the lesions may be nodules, plaques, or tumorous masses. mycetomas are caused by either true fungi (eumycetes) or filamentous bacteria (actinomycetes); they also occur predominantly in tropical areas and ... | 1995 | 7829748 |
| prevalence of fungal corneal ulcers in northern india. | fungal corneal ulcer is common in india due to the tropical climate and a large agrarian population that is at risk. various factors such as trauma, the injudicious use of topical antibiotics and corticosteroids are involved. many of the age and sex-related risk factors also play a minor role. this 6-year study from northern india revealed that fungi were detected in 61 (8.4%) out of 730 patients investigated. direct microscopy was positive in 51 (7%) and culture in 53 (7.3%) patients. aspergill ... | 1994 | 7927819 |
| experience with brain abscess in the central province of saudi arabia. | the characteristics of brain abscess in the central province of saudi arabia are outlined in this report which is a review of 22 consecutive cases that were treated at king khalid university hospital between 1985-1991. the incidence of brain abscess in saudi arabia is calculated to be 3.6 cases/500,000 population/year which is twice as high as the incidence reported from the west. there were 17 males and 5 females with an age range of 8 months-80 years (mean 29.9 years). the parietal lobe was in ... | 1994 | 7941005 |
| the in vitro susceptibility of fonsecaea pedrosoi to activated macrophages. | light and electron microscopy were used to analyze in vitro the interaction of fonsecaea pedrosoi with in vivo activated-macrophages. adherence of the fungi to the surface of activated macrophages triggers the respiratory burst as revealed by reduction of nitroblue tetrazolium. transmission electron microscopy revealed nad(po)h-oxidase activity in the portions of the macrophage plasma of membrane that were in contact with the fungus as well as within phagocytic vacuoles. activated macrophages fa ... | 1994 | 8065435 |
| treatment of tropical mycoses. | several subcutaneous and deep-seated mycoses are either observed more frequently in the tropical areas or are restricted to certain regions within the tropics. these mycoses include sporotichosis, chromoblastomycosis, entomophthoromycosis, eumycetoma, lobomycosis, and paracoccidioidomycosis. in sporotrichosis and paracoccidioidomycosis, therapy often results in either complete resolution or marked improvement. for decades sporotrichosis has been treated successfully with potassium iodide, but re ... | 1994 | 8077517 |
| [chromomycosis: 2 cases]. | we report two cases of chromomycosis due to fonsecaea pedrosoi and cladosporium herbarum, observed in two women from the tlemcen region. due to the wart-like or nodular lesions, these case were mistaken for other skin diseases with similar manifestations, particularly verrucous tuberculosis and cutaneous leishmaniasis. the histology revealed fumagoid bodies leading to the identification of the causative agents. chromomycosis is rare in algeria although 3 cases have been observed in our area sinc ... | 1994 | 8092726 |
| a preliminary survey of cycloheximide-resistant airborne fungi in turin, italy. | numbers and type of the cycloheximide-resistant part of the aerially transmitted mycoflora of turin were studied. samples from three areas characterized by differing human usage were taken in the first week of march. during each sampling, 12 m3 of air were aspirated, using an one-stage volumetric sieve sampler. fifty-two mesophilic species and eight thermotolerant were isolated. propagule load varied from 2.92 to 120.31 cfu m-3. the following species appear not to have been reported previously f ... | 1993 | 8247094 |
| calcium regulates in vitro dimorphism in chromoblastomycotic fungi. | cladosporium carrionii, fonsecaea pedrosoi and phialophora verrucosa, the three most important agents of chromoblastomycosis, produced large numbers of sclerotic bodies at 25 degrees c, and greater numbers at 37 degrees c, after inoculation into a defined ph 2.5 medium containing 0.1 mmol l-1 ca2+. higher concentrations of ca2+ reversed this tendency and promoted maintenance of hyphal growth. addition of the ca2+ chelator egta to the same medium buffered at ph 6.5 also induced sclerotic bodies, ... | 1993 | 8264711 |
| hyperthermic treatment of chromomycosis with disposable chemical pocket warmers. report of a successfully treated case, with a review of the literature. | a case of chromomycosis in which hyperthermia proved effective is reported. the patient was a 56-year-old male bean curd maker who, without any previous history of minor trauma, developed on the extensor side of the left upper arm an eczematous lesion that underwent gradual radial expansion. the lesion showed a well-defined, 7 x 10 cm infiltrated erythematous plaque with the central area healed and, at the upper and lower borders, adherent scales and crusts on the surface. histological examinati ... | 1993 | 8326999 |
| [a cutaneous parasitosis rarely observed in france: chromomycosis]. | the authors report a case of chromomycosis presenting like a tumoral lesion in a patient coming from the isle de mayotte. the causative agent is fonsecaea pedrosoi. the evolution was favorable after surgical treatment. the epidemiology, the histopathological features, the diagnosis problems and the evolution of the illness are studied. | 1993 | 8363672 |
| phaeohyphomycosis of the paranasal sinuses masquerading as a neoplasm: a case report. | a case of fungal infection of the paranasal sinuses simulating a neoplasm is reported. fonsecaea pedrosoi, a dematiacious fungus, which has not been previously reported, was responsible for this unusual infection. fungal infection may mimic malignant lesions of the paranasal sinuses; however, coexisting malignancy must be ruled out. | 1993 | 8416859 |
| mitochondrial dna analysis of exophiala spinifera. | restriction fragment length polymorphism (rflp) of mitochondrial dna (mtdna) was examined in 36 isolates of exophiala spinifera (8 isolates from brazil, 9 from china, 15 from columbia, 1 from the united states and 2 from venezuela). e. spinifera isolates displayed a high degree of mtdna diversity in rflp patterns and were clustered into six genetically heterogeneous groups (group 1 through group 6). isolates of group 2 including the type strain seemed to have a worldwide distribution. rflp patte ... | 1995 | 8532056 |
| successful treatment of chromoblastomycosis due to fonsecaea pedrosoi by the combination of itraconazole and cryotherapy. | many methods and medications had been tried for the treatment of chromoblastomycosis with unsatisfactory results. recently, there were several reports showing a good response of chromoblastomycosis to itraconazole, but it took as long as 18-30 months for lesions to heal. | 1995 | 8543418 |
| an exoantigen test for the rapid identification of medically significant fusarium species. | the accurate identification of fusarium species can take 2-3 weeks. preliminary exoantigen studies indicate that a mature culture suspected of being a fusarium species may be immunologically identified 48 h after receipt. exoantigen extracts of 10-day-old slant cultures of fusarium chlamydosporum, fusarium moniliforme (= fusarium verticilloides), fusarium oxysporum, fusarium proliferatum and fusarium solani and partially purified reference homologous and heterologous shake culture extracts (6-we ... | 1995 | 8544080 |
| pneumonia due to fonsecaea pedrosoi and cerebral abscesses due to emericella nidulans in a bone marrow transplant recipient. | | 1995 | 8589181 |
| forty years of chromoblastomycosis in madagascar: a review. | confirmed cases of chromoblastomycosis in madagascar from 1955 through 1994 were studied retrospectively. the total number of cases reported was 1,343, of which 98.5% were confirmed by histopathology. only 30.8% of the cases showed a positive cultivation on mycologic media, and fonsecaea pedrosoi was identified from 61.8% of the fungal strains. two distinct areas of endemic chromoblastomycosis, each with a characteristic ecosystem and a single species, are identified. madagascar represents the m ... | 1996 | 8702021 |
| identification of the conserved coding sequences of three chitin synthase genes in fonsecaea pedrosoi. | primers having designs based on highly conserved stretches in the deduced amino acid sequences of chitin synthase (chs) genes were used in pcr reactions to amplify 600 bp and 366 bp products from the genomic dna of three major causal agents of chromoblastomycosis. cloning and sequencing of the pcr products of one of these fungi, fonsecaea pedrosoi, identified three chs sequences designated as fpchs1, fpchs2 and fpchs3. fpchs1 and fpchs2 were homologous to regions of chs1 and chs2 of saccharomyce ... | 1996 | 8732357 |
| treatment of chromomycosis with terbinafine: preliminary results of an open pilot study. | in an open trial, long courses (6-12 months) of terbinafine at a dosage of 500 mg/day were administered orally to 43 patients with a diagnosis of chromomycosis. sixteen patients (37.2%) had previously relapsed after one or two courses of thiabendazole. a spectacular improvement in the lesions, including disappearance of bacterial superinfections and of associated oedema and elephantiasis, was observed as soon as 2-4 months after the beginning of treatment. the mean number of fungal cells in skin ... | 1996 | 8763467 |
| carbohydrate and lipid components of hyphae and conidia of human pathogen fonsecaea pedrosoi. | the carbohydrate and lipid components of mycelium and conidia of fonsecaea pedrosoi (brumpt) were analysed by paper, thin-layer and gas-chromatography, mass spectrometry and ultraviolet spectroscopy. glucose, mannose, galactofuranose, rhamnose and glucosamine were polysaccharide components identified in f. pedrosoi. significant changes in the carbohydrate pattern occurred during the conversion of mycelium into conidia. rhamnose was predominant in conidia whereas galactose was prominent in myceli ... | 1995 | 8819829 |
| persistent viability of the medlar body. | the medlar body represents an adaptive tissue form of the fungi known to cause cutaneous chromomycosis. this study was designed to determine the in vitro viability of medlar bodies that are found in profusion within lesional epidermis. | 1996 | 8850035 |
| fine structure and cytochemical study of the interaction between fonsecaea pedrosoi and rat polymorphonuclear leukocyte. | the induction of a granulomatous reaction is frequently observed in subcutaneous mycoses. our previous studies demonstrated that fonsecaea pedrosoi was able to survive and proliferate in tissue macrophages and that activated macrophages were fungistatic but not fungicidal. by contrast, our present studies revealed that neutrophils were able to kill f. pedrosoi cells in periods shorter than 20 min. several phases of the interaction process were analysed by light and electron microscopy. the kinet ... | 1996 | 8912165 |
| a case of chromomycosis with tumor-like growth. | a 66-year-old woman who lived on tokunoshima island, a small and remote southern island of the japanese archipelago, had suffered from chromomycosis for more than 30 years and presented with a tumor-like growth on the posterior crural region of his right leg. fonsecaea pedrosoi was identified as the pathogen from its growth pattern and micromorphological characteristics. the patient was successfully treated with 5-fluorocytosine, itoraconazole, and topical thermotherapy. | 1996 | 8916668 |
| corneal chromoblastomycosis. | we sought to illustrate the difficulty in managing uncommon, pigmented mold-related corneal ulceration and to highlight the role of itraconazole in treating these patients. | 1997 | 9071540 |
| in vitro antifungal activity of pneumocandin l-743,872 against a variety of clinically important molds. | the in vitro activity of the new antifungal drug pneumocandin l-743,872 against 55 isolates of clinically important molds was examined by an adapted macrobroth dilution method for yeasts. pneumocandin l-743,872 exhibited in vitro antifungal activity against alternaria sp., aspergillus flavus, aspergillus fumigatus, curvularia lunata, exophiala jeanselmei, fonsecaea pedrosoi, paecilomyces variotii, and scedosporium apiospermum. the drug appeared to lack significant in vitro inhibitory activity ag ... | 1997 | 9257774 |
| [chromoblastomycosis in rio grande do sul: a report of 12 cases]. | twelve cases of chromoblastomycosis diagnosed in rio grande do sul during 1988-1995 are reported. the clinical aspects are analyzed and compared with the literature. fonsecaea pedrosoi was the only microorganism isolated. | 1997 | 9265226 |
| treatment of chromoblastomycosis with itraconazole, cryosurgery, and a combination of both. | chromoblastomycosis is a subcutaneous mycosis, seen frequently in tropical areas, and caused by dematiaceous fungi. it produces nodulo-verrucous lesions in the arms and legs. there is no treatment of choice for this disease and sometimes a combination of chemotherapy and physical therapy is necessary. | 1997 | 9268758 |
| chromoblastomycosis murine model and in vitro test to evaluate the sensitivity of fonsecaea pedrosoi to ketoconazole, itraconazole and saperconazole. | an experimental model of murine chromoblastomycosis and in vitro tests with fonsecaea pedrosoi were used to test the sensitivity of this fungus to three different antimycotics. the experimental model was standardized in balb/c mice inoculated intraperitoneally with a 10(6) cfu/ml suspension of a f. pedrosoi isolate. clinical infection was evident after 5 days of inoculation. three groups of 27 mice each were used in the experiment. one group was treated with ketoconazole (ktz), another with itra ... | 1996 | 9283666 |
| chromoblastomycosis--a clinical and mycological study of 71 cases from sri lanka. | chromoblastomycosis, a well-documented chronic fungal infection, represents a specific clinical entity with typical warty cutaneous nodules and a worldwide distribution. although more prevalent in tropical and subtropical regions, only a few reports are available from sri lanka or from asia. five etiologic agents of chromoblastomycosis have been recognized worldwide. of these the majority of infections have been caused by fonsecaea pedrosoi. during the period from 1952 to 1962, only twelve cultu ... | 1997 | 9368408 |