[studies of leishmania gerbilli in china]. 19854053322
[occurrence of leishmania gerbilli in uradhouqi, nei mongol]. 19836678651
consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating leishmania genotypes.recently, a new polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp)-based assay had been developed using the miniexon sequences for genotyping leishmania isolates. we had used this method for rapid diagnosis and genotyping of visceral and cutaneous leishmaniasis with the combination of microcapillary cultivation. in this study, we have evaluated this approach by examining genomic dnas from 47 independent isolates, which were grouped into 19 genotypes of leishmania sub ...200717141456
effect of leishmania gerbilli injection on mice immunization against cutaneous leishmaniasis (cl) caused by leishmania the present study leishmania gerbilli were used to immunize balb/c mice against pathogenic strains of leishmania to determine whether injection of l. gerbilli in mice could protect them against later l. major inoculation. eighty female balb/c mice were divided by random in eight groups. promastigotes of l. major and l. gerbilli were used. mice were inoculated with three different doses of l. gerbilli (3 x 10(6), 2 x 10(7) and 5 x 10(7)) via subcutaneous (sc) in the base of their tails or inte ...200719070016
leishmania species: detection and identification by nested pcr assay from skin samples of rodent reservoirs.many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. in the present study a simple and reliable assay based on nested pcr was developed for the detection and identification of leishmania parasites from rodent skin samples. we designed leishmania-specific primers that successfully amplified its regions of leishmania major, leishmania gerbilli and leishmania turanica using nested pcr. out of 95 field collected rhombomys opimus, 21 were positive by micros ...201020566364
species delimitation and phylogenetic relationships of chinese leishmania isolates reexamined using kinetoplast cytochrome oxidase ii gene sequences.leishmaniasis is a geographically widespread disease caused by protozoan parasites belonging to the genus leishmania and transmitted by certain species of sand fly. this disease still remains endemic in china, especially in the west and northwest frontier regions. a recent its1 phylogeny of chinese leishmania isolates has challenged some aspects for their traditional taxonomy and cladistic hypotheses of their phylogeny. however, disagreement with respect to relationships within chinese leishmani ...201121221640
analysis of kinetoplast cytochrome b gene of 16 leishmania isolates from different foci of china: different species of leishmania in china and their phylogenetic inference.leishmania species belong to the family trypanosomatidae and cause leishmaniasis, a geographically widespread disease that infects humans and other vertebrates. this disease remains endemic in china. due to the large geographic area and complex ecological environment, the taxonomic position and phylogenetic relationship of chinese leishmania isolates remain uncertain. a recent internal transcribed spacer 1 and cytochrome oxidase ii phylogeny of chinese leishmania isolates has challenged some asp ...201323383990
molecular detection of leishmania infection in sand flies in border line of iran-turkmenistan: restricted and permissive vectors.a molecular study was carried out to incriminate sand fly vectors of cutaneous leishmaniasis (cl) in rural areas of sarakhs district, khorassane-razavi province, northeastern iran, in 2011. sand flies of sergentomyia with three species and phlebotomus with six species respectively comprised 73.3% and 26.7% of the specimens. phlebotomus papatasi was the most common phlebotomine species in outdoor and indoor resting places. leishmania infection was found at least in 17 (22%) specimens including ph ...201323933280
phylogenetic analysis of lack gene sequences for 22 chinese leishmania isolates.the phylogenetic relationships between chinese leishmania strains were investigated using lack (leishmania homolog of receptors for activated protein kinase c) gene sequences, and the power of this gene was assessed for understanding the epidemiology and population genetics of leishmania.201323541410
identification and phylogenetic relationship of iranian strains of various leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on n-acetylglucosamine-1-phosphate transferase gene.the identity of iranian leishmania species has been resolved to some extent by some genetic markers. in this study, based on n-acetylglucosamine-1-phosphate transferase (nagt) gene, we further elucidated the identity and phylogeny of the prevalent species in this country. dnas of 121 isolates belonging to cutaneous leishmaniasis (cl) patients, canine visceral leishmaniasis (cvl) cases, and rhombomys opimus rodents were amplified by targeting a partial sequence of nagt gene. all the amplicons wer ...201424911282
high density of leishmania major and rarity of other mammals' leishmania in zoonotic cutaneous leishmaniasis foci, iran.only leishmania major is well known as a causative agent of zoonotic cutaneous leishmaniasis (zcl) in iran. our objective was to find leishmania parasites circulating in reservoir hosts, sand flies and human simultaneously.201424382378
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