| acetylaminofluorene-labelled ribosomal rna for use in molecular epidemiology and taxonomy. | the use of acetylaminofluorene-labelled 16 + 23s rrna (from escherichia coli) is described for determining rrna-gene-restriction patterns. the labelled probe allowed molecular fingerprinting of bacteria belonging to diverse phylogenetic branches (enterobacteriaceae, haemophilus, pseudomonas, acinetobacter, brucella, leptospira, cytophaga, campylobacter, methylophaga). the labelled probe can be stored frozen (-20 degrees c) for at least a year and can endure vacuum dessication, ethanol precipitat ... | 1989 | 2696056 |
| purification and properties of methanol dehydrogenase from methylophaga marina. | purification of methanol dehydrogenase from methylophaga marina, in order to avoid the instability observed in crude extracts, was achieved initially by a rapid procedure using mainly an aqueous two-phase partition system composed of polyethylene glycol 1000 (50%, v/v) and potassium phosphate (50%, w/v). the purified enzyme gave a single band of protein after sds-polyacrylamide gel electrophoresis. antiserum raised against purified methanol dehydrogenase was used to detect possible protein conta ... | 1987 | 3129021 |
| the genus methylophaga, a new line of descent within phylogenetic branch gamma of proteobacteria. | the genus methylophaga with two species, m. marina and m. thalassica, comprises halophilic methylotrophic bacteria. these organisms utilise c1 compounds through the ribulose monophosphate pathway and are unable to grow on methane. nearly complete 16s rrna sequences were obtained for both methylophaga species by directly sequencing the amplified 16s rrna gene. these sequences were compared with published 16s rrna sequences of methylotrophic strains and a large number of marine bacterial strains i ... | 1995 | 8577995 |
| genetic analysis and complete nucleotide sequence of a 2-kb cryptic plasmid from the marine methylotroph methylophaga thalassica s1. | a small cryptic plasmid (pmts1) was isolated from the marine methylotroph methylophaga thalassica s1. sequence analysis showed that pmts1 has a 1995-bp genome encoding three putative polypeptides and containing two intergenic regions. the longest open reading frame encodes a polypeptide of 325 amino acid (37,079 da). this polypeptide shares similarity and signature amino acids with the rep proteins of the pc194 group replicons, suggesting that pmts1 replicates by the rolling-cycle mechanism. ins ... | 2000 | 10873533 |
| characterization of the conserved region of the mxaf gene that encodes the large subunit of methanol dehydrogenase from a marine methylotrophic bacterium. | the highly conserved region of the mxaf gene that encodes the large subunit of methanol dehydrogenase (mdh) was cloned and sequenced from methylophaga sp. strain mp cells. the calculated g + c content of the conserved region was found to be 44.9%. the nucleotide sequence homology of the region to those from methylotrophs was approximately 43.5%, while the identity of the deduced amino acid sequence to other mxaf peptides was approximately 76.8%. analysis of the codon usage revealed that uuc and ... | 2002 | 12132575 |
| purification and characterization of two forms of methanol dehydrogenases from a marine methylotroph. | two methanol dehydrogenases (mdhs), mdh1 and mdh2, were purified from a marine methylotroph, methylophaga sp. strain 1. both enzymes had very similar properties, including the same native molecular weight, sizes of subunits and substrate specificity. the n-terminal amino acid sequence of the alpha-subunit of mdh2 differed from that of mdh1 by having a histidine residue at a highly conserved glutamate position, but both sequences showed approximately 50% homology to the alpha-subunits of other md ... | 2002 | 12210547 |
| methylophaga alcalica sp. nov., a novel alkaliphilic and moderately halophilic, obligately methylotrophic bacterium from an east mongolian saline soda lake. | a moderately haloalkaliphilic and obligately methylotrophic bacterium (strain m39t) with the ribulose monophosphate pathway of carbon assimilation is described. cells of this methanol and methylamine utilizer are aerobic, gram-negative, asporogenous, motile short rods, multiplying by binary fission. it is auxotrophic for vitamin b12 and requires nahco3 or nacl for growth in alkaline medium. its cellular fatty acid profile consists primarily of straight-chain saturated c16:0 and unsaturated c16:1 ... | 2003 | 12656177 |
| bacterial diversity in a marine methanol-fed denitrification reactor at the montreal biodome, canada. | the bacterial biota of a methanol-fed denitrification reactor used to treat seawater at the montreal biodome were investigated using culture-dependent and molecular biology methods. the microbiota extracted from the reactor carriers were cultivated on three media. three isolate types were recovered and their 16s ribosomal dna (rdna) genes were determined. the analysis showed that the isolate types were related to alpha-proteobacteria. they are members of the hyphomicrobium and paracoccus genera ... | 2003 | 12739074 |
| a novel flavin-containing monooxygenase from methylophaga sp strain sk1 and its indigo synthesis in escherichia coli. | we cloned a gene from methylophaga sp. strain sk1. this gene was responsible for producing, the blue pigment, indigo. the complete open reading frame was 1371 bp long, which encodes a protein of 456 amino acids. the molecular mass of the encoded protein was 105 kda, consisting of homodimer of 54 kda with an isoelectric point of 5.14. the deduced amino acid sequence from the gene showed approximately 30% identities with flavin-containing monooxygenases (fmos) of human (fmo1-fmo5), arabidopsis, an ... | 2003 | 12821131 |
| development and use of fluorescent 16s rrna-targeted probes for the specific detection of methylophaga species by in situ hybridization in marine sediments. | methylotrophic bacteria are widespread in nature. they may play an important role in the cycling of carbon and in the metabolism of dimethylsulfide in a marine environment. bacteria belonging to the genus methylophaga are a unique group of aerobic, halophilic, non-methane-utilizing methylotrophs. two 16s rrna-targeted oligonucleotide probes were developed for the specific detection of methylophaga species, marine methylobacteria, by fluorescence in situ hybridization. probe mph-730 was highly sp ... | 2003 | 14499934 |
| methylophaga natronica sp. nov., a new alkaliphilic and moderately halophilic, restricted-facultatively methylotrophic bacterium from soda lake of the southern transbaikal region. | a new, moderately haloalkaliphilic and restricted-facultatively methylotrophic bacterium (strain bur2t) with the ribulose monophosphate pathway of carbon assimilation is described. the isolate, which utilizes methanol, methylamine and fructose, is an aerobic, gram-negative, asporogenous, motile short rod multiplying by binary fission. it is auxotrophic for vitamin b12, and requires nahco3 or nacl for growth in alkaline medium. cellular fatty acids profile consists primarily of straight-chain sat ... | 2003 | 14529181 |
| changes in bacterioplankton composition under different phytoplankton regimens. | the results of empirical studies have revealed links between phytoplankton and bacterioplankton, such as the frequent correlation between chlorophyll a and bulk bacterial abundance and production. nevertheless, little is known about possible links at the level of specific taxonomic groups. to investigate this issue, seawater microcosm experiments were performed in the northwestern mediterranean sea. turbulence was used as a noninvasive means to induce phytoplankton blooms dominated by different ... | 2004 | 15528542 |
| purification and characterization of a novel atp-independent type i dna topoisomerase from a marine methylotroph. | dna topoisomerase is involved in dna repair and replication. in this study, a novel atp-independent 30-kda type i dna topoisomerase was purified and characterized from a marine methylotroph, methylophaga sp. strain 3. the purified enzyme composed of a single polypeptide was active over a broad range of temperature and ph. the enzyme was able to relax only negatively supercoiled dna. mg(2+) was required for its relaxation activity, while atp gave no effect. the enzyme was clearly inhibited by cam ... | 2005 | 15850556 |
| [methylophaga murata sp. nov.: a haloalkaliphilic aerobic methylotroph from deteriorating marble]. | the haloalkaliphilic methylotrophic bacterium (strain kr3) isolated from material scraped off the deteriorating marble of the moscow kremlin masonry has been found to be able to utilize methanol, methylamine, trimethylamine, and fructose as carbon and energy sources. its cells are gram-negative motile rods multiplying by binary fission. spores are not produced. the isolate is strictly aerobic and requires vitamin b12 and na+ ions for growth. it is oxidase- and catalase-positive and reduces nitra ... | 2005 | 16211855 |
| characterization of methylophaga sp. strain sk1 cytochrome cl expressed in escherichia coli. | methylophaga sp. strain sk1 is a new restricted facultative methanol-oxidizing bacterium that was isolated from seawater. the aim of this study was to characterize the electron carriers involved in the methanol oxidation process in methylophaga sp. strain sk1. the gene encoding cytochrome c(l) (mxag) was cloned and the recombinant gene was expressed in escherichia coli dh5 under strict anaerobic conditions. the recombinant cytochrome c(l) had the same molecular weight and absorption spectra as t ... | 2005 | 16410765 |
| competition for dimethyl sulfide and hydrogen sulfide by methylophaga sulfidovorans and thiobacillus thioparus t5 in continuous cultures. | pure and mixed cultures of methylophaga sulfidovorans and thiobacillus thioparus t5 were grown in continuous cultures on either dimethyl sulfide, dimethyl sulfide and h(inf2)s, or h(inf2)s and methanol. in pure cultures, m. sulfidovorans showed a lower affinity for sulfide than t. thioparus t5. mixed cultures, grown on dimethyl sulfide, showed coexistence of both species. m. sulfidovorans fully converted dimethyl sulfide to thiosulfate, which was subsequently further oxidized to sulfate by t. th ... | 1997 | 16535680 |
| phylogenetic identification and metabolism of marine dimethylsulfide-consuming bacteria. | microbial consumption is one of the main processes, along with photolysis and ventilation, that remove the biogenic trace gas dimethylsulfide (dms) from the surface ocean. although a few isolates of marine bacteria have been studied for their ability to utilize dms, little is known about the characteristics or phylogenetic affiliation of dms consumers in seawater. we enriched coastal and open-ocean waters with different carbon sources to stimulate different bacterial communities (glucose-consumi ... | 2006 | 17107560 |
| isolation of methylophaga spp. from marine dimethylsulfide-degrading enrichment cultures and identification of polypeptides induced during growth on dimethylsulfide. | dimethylsulfide (dms)-degrading enrichment cultures were established from samples of coastal seawater, nonaxenic emiliania huxleyi cultures, and mixed marine methyl halide-degrading enrichment cultures. bacterial populations from a broad phylogenetic range were identified in the mixed dms-degrading enrichment cultures by denaturing gradient gel electrophoresis (dgge). sequences of dominant dgge bands were similar to those of members of the genera methylophaga and alcanivorax. several closely rel ... | 2007 | 17322322 |
| diversity and abundance of aerobic and anaerobic methane oxidizers at the haakon mosby mud volcano, barents sea. | submarine mud volcanoes are formed by expulsions of mud, fluids, and gases from deeply buried subsurface sources. they are highly reduced benthic habitats and often associated with intensive methane seepage. in this study, the microbial diversity and community structure in methane-rich sediments of the haakon mosby mud volcano (hmmv) were investigated by comparative sequence analysis of 16s rrna genes and fluorescence in situ hybridization. in the active volcano center, which has a diameter of a ... | 2007 | 17369343 |
| microbiological community structure of the biofilm of a methanol-fed, marine denitrification system, and identification of the methanol-utilizing microorganisms. | we demonstrated in a previous study that the biofilm of the methanol-fed fluidized marine denitrification reactor at the montreal biodome was composed of at least 15 bacterial phylotypes. among those were 16s ribosomal rna (rdna) gene sequences affiliated to hyphomicrobium spp., and methylophaga spp.; the latter made up 70% of a clone library. by using fluorescent in situ hybridization (fish), we investigated the structure of the biofilm during the colonization process in the denitrification rea ... | 2007 | 17394042 |
| [vitamin b12-independent strains of methylophaga marina isolated from red sea algae]. | two strains (km3 and km5) of halophilic methylobacteria isolated from red sea algae do not require vitamin b12 for growth and can use methanol, methylamine, dimethylamine, trimethylamine, dimethyl sulfide, and fructose as sources of carbon and energy. the cells of these strains are gram-negative motile monotrichous (strain km3) or peritrichous (strain km5) rods. the strains are strictly aerobic and require na+ ions but not growth factors for growth. they are oxidase- and catalase-positive and re ... | 2007 | 17410879 |
| a novel lineage of proteobacteria involved in formation of marine fe-oxidizing microbial mat communities. | for decades it has been recognized that neutrophilic fe-oxidizing bacteria (feob) are associated with hydrothermal venting of fe(ii)-rich fluids associated with seamounts in the world's oceans. the evidence was based almost entirely on the mineralogical remains of the microbes, which themselves had neither been brought into culture or been assigned to a specific phylogenetic clade. we have used both cultivation and cultivation-independent techniques to study fe-rich microbial mats associated wit ... | 2007 | 17668050 |
| methylophaga aminisulfidivorans sp. nov., a restricted facultatively methylotrophic marine bacterium. | a novel restricted facultatively methylotrophic marine strain, mp(t), possessing the ribulose monophosphate pathway of c(1)-carbon compound assimilation was isolated from a seawater sample obtained from mokpo, south korea. the novel isolate is aerobic, gram-negative, asporogenous and a non-motile short rod. it grows well on methanol, methylated amines, dimethylsulfide and dmso. optimal growth occurs with 3 % nacl at 30 degrees c and ph 7.0. fructose is utilized as a multicarbon source. growth fa ... | 2007 | 17766878 |
| stable-isotope probing implicates methylophaga spp and novel gammaproteobacteria in marine methanol and methylamine metabolism. | the metabolism of one-carbon (c(1)) compounds in the marine environment affects global warming, seawater ecology and atmospheric chemistry. despite their global significance, marine microorganisms that consume c(1) compounds in situ remain poorly characterized. stable-isotope probing (sip) is an ideal tool for linking the function and phylogeny of methylotrophic organisms by the metabolism and incorporation of stable-isotope-labelled substrates into nucleic acids. by combining dna-sip and time-s ... | 2007 | 18043650 |
| marine methylotrophs revealed by stable-isotope probing, multiple displacement amplification and metagenomics. | the concentrations of one-carbon substrates that fuel methylotrophic microbial communities in the ocean are limited and the specialized guilds of bacteria that use these molecules may exist at low relative abundance. as a result, these organisms are difficult to identify and are often missed with existing cultivation and gene retrieval methods. here, we demonstrate a novel proof of concept: using environmentally-relevant substrate concentrations in stable-isotope probing (sip) incubations to yie ... | 2008 | 18294205 |
| impact of nitrate on the structure and function of bacterial biofilm communities in pipelines used for injection of seawater into oil fields. | we studied the impact of no(3)(-) on the bacterial community composition, diversity, and function in in situ industrial, anaerobic biofilms by combining microsensor profiling, (15)n and (35)s labeling, and 16s rrna gene-based fingerprinting. biofilms were grown on carbon steel coupons within a system designed to treat seawater for injection into an oil field for pressurized oil recovery. no(3)(-) was added to the seawater in an attempt to prevent bacterial h(2)s generation and microbially influe ... | 2008 | 18344353 |
| characterization of the recombinant diaminobutyric acid acetyltransferase from methylophaga thalassica and methylophaga alcalica. | diaminobutyric acid acetyltransferase (ecta) catalyzes the acetylation of diaminobutyric acid to gamma-n-acetyl-alpha,gamma-diaminobutyrate with acetyl coenzyme a. this is the second reaction in the ectoine biosynthetic pathway. the recombinant ecta proteins were purified from two moderately halophilic methylotrophic bacteria: methylophaga thalassica atcc 33146t and methylophaga alcalica atcc 35842t. ecta found in both methylotrophs is a homodimer with a subunit molecular mass of c. 20 kda and h ... | 2008 | 18410346 |
| revealing the moonlighting role of nadp in the structure of a flavin-containing monooxygenase. | flavin-containing monooxygenases (fmos) are, after cytochromes p450, the most important monooxygenase system in humans and are involved in xenobiotics metabolism and variability in drug response. the x-ray structure of a soluble prokaryotic fmo from methylophaga sp. strain sk1 has been solved at 2.6-a resolution and is now the protein of known structure with the highest sequence similarity to human fmos. the structure possesses a two-domain architecture, with both fad and nadp(+) well defined by ... | 2008 | 18443301 |
| effects of carbon source on denitrification efficiency and microbial community structure in a saline wastewater treatment process. | two different denitrifying reactors were monitored in order to evaluate the effects of carbon source on denitrification efficiency and microbial community structure under various saline conditions. nitrogen removal performances were determined when salinity concentrations increase gradually in acetate- or methanol-fed denitrifying reactor. as a result, acetate-fed process attained high nitrate removal at 0-10% nacl, while methanol was proven beneficial electron donors at 0-3% nacl. a parallel an ... | 2008 | 18675439 |
| methane assimilation and trophic interactions with marine methylomicrobium in deep-water coral reef sediment off the coast of norway. | deep-water coral reefs are seafloor environments with diverse biological communities surrounded by cold permanent darkness. sources of energy and carbon for the nourishment of these reefs are presently unclear. we investigated one aspect of the food web using dna stable-isotope probing (dna-sip). sediment from beneath a lophelia pertusa reef off the coast of norway was incubated until assimilation of 5 micromol 13ch4 g(-1) wet weight occurred. extracted dna was separated into 'light' and 'heavy' ... | 2008 | 18811651 |
| substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment. | marine microorganisms that consume one-carbon (c(1)) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry. this study investigated marine bacterial communities involved in the metabolism of c(1) compounds. these communities were of relevance to surface seawater and atmospheric chemistry in the context of a bloom that was dominated by phytoplankton known to produce dimethylsulfoniopropionate. in addition to using 16s rrna gen ... | 2008 | 18849453 |
| characterization of sulfoxygenation and structural implications of human flavin-containing monooxygenase isoform 2 (fmo2.1) variants s195l and n413k. | catalytically active human flavin-containing monooxygenase isoform 2 (fmo2.1) is encoded by an allele detected only in individuals of african or hispanic origin. genotyping and haplotyping studies indicate that s195l and n413k occasionally occur secondary to the functional fmo2*1 allele encoding reference protein gln472. sulfoxygenation under a range of conditions reveals the role these alterations may play in individuals expressing active fmo2 and provides insight into fmo structure. expressed ... | 2009 | 19420133 |
| ectr--a novel transcriptional regulator of ectoine biosynthesis genes in the haloalcaliphilic methylotrophic bacterium methylophaga alcalica. | | 2009 | 20101826 |
| [biosynthesis of the bioprotectant ectoin by aerobic methylotrophic bacteria from methanol]. | it is shown that neutrophilic methylobacteria methylophaga thalassica and m. marina have higher rates of growth and ectoin accumulation compared to the haloalkaliphilic species m. alcalica and m. natronia and methanotrophs methylomicrobium alcaliphilum and m. kenyense. the conditions of m. thalassica cultivation in methanol-containing medium were optimized. the yield of this process reached 60 g/l of absolutely dry biomass containing 15-19% (9-11 g/l) ectoine. the scheme of ectoin isolation from ... | 2010 | 20391762 |
| dissimilatory reduction of nitrate in seawater by a methylophaga strain containing two highly divergent narg sequences. | methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. they have been shown previously to represent 50-70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the montreal biodome. it was therefore surprising to find such a high concentration of methylophaga spp. in anoxic conditions. in this study, we showed by cultivation-independent and -dep ... | 2010 | 20393572 |
| oxidation of dimethylsulfide to tetrathionate by methylophaga thiooxidans sp. nov.: a new link in the sulfur cycle. | a new pathway of dimethylsulfide (dms) metabolism was identified in a novel species of gammaproteobacteria, methylophaga thiooxidans sp. nov., in which tetrathionate (s(4)o(6)(2-)) was the end-product of dms oxidation. inhibitor evidence indicated that dms degradation was initiated by demethylation, catalysed by a corrinoid demethylase. thiosulfate was an intermediate, which was oxidized to tetrathionate by a cytochrome-linked thiosulfate dehydrogenase. thiosulfate oxidation was coupled to atp s ... | 2010 | 20482741 |
| microbial community structure of a heavy fuel oil-degrading marine consortium: linking microbial dynamics with polycyclic aromatic hydrocarbon utilization. | a marine microbial consortium obtained from a beach contaminated by the prestige oil spill proved highly efficient in removing the different hydrocarbon families present in this heavy fuel oil. seawater cultures showed a complete removal of all the linear and branched alkanes, an extensive attack on three to five-ring polycyclic aromatic hydrocarbons [pahs; including anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, and benzo(a)pyrene] (30-100%), and a considerable depletion of the ... | 2010 | 20528986 |
| active methylotrophs in the sediments of lonar lake, a saline and alkaline ecosystem formed by meteor impact. | lonar lake is a unique saline and alkaline ecosystem formed by meteor impact in the deccan basalts in india around 52,000 years ago. to investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (sip) was carried out using the one-carbon compounds methane, methanol and methylamine. denaturing gradient gel electrophoresis fingerprinting analyses performed with heavy (13)c-labelled dna retrieved from sediment microcosms confirmed the enrichme ... | 2010 | 20555363 |
| identification of novel methane-, ethane-, and propane-oxidizing bacteria at marine hydrocarbon seeps by stable isotope probing. | marine hydrocarbon seeps supply oil and gas to microorganisms in sediments and overlying water. we used stable isotope probing (sip) to identify aerobic bacteria oxidizing gaseous hydrocarbons in surface sediment from the coal oil point seep field located offshore of santa barbara, california. after incubating sediment with (13)c-labeled methane, ethane, or propane, we confirmed the incorporation of (13)c into fatty acids and dna. terminal restriction fragment length polymorphism (t-rflp) analys ... | 2010 | 20675448 |
| microbial community transcriptomes reveal microbes and metabolic pathways associated with dissolved organic matter turnover in the sea. | marine dissolved organic matter (dom) contains as much carbon as the earth's atmosphere, and represents a critical component of the global carbon cycle. to better define microbial processes and activities associated with marine dom cycling, we analyzed genomic and transcriptional responses of microbial communities to high-molecular-weight dom (hmwdom) addition. the cell density in the unamended control remained constant, with very few transcript categories exhibiting significant differences over ... | 2010 | 20807744 |
| genes and enzymes of ectoine biosynthesis in halotolerant methanotrophs. | ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) is a widely distributed compatible solute accumulated by halophilic and halotolerant microorganisms to prevent osmotic stress in highly saline environments. ectoine as a highly water keeping compound stabilizing biomolecules and whole cells can be used in scientific work, cosmetics, and medicine. detailed understanding of the organization/regulation of the ectoine biosynthetic pathway in various producers is an active area of res ... | 2011 | 21419912 |
| draft genome sequence of the chemolithoheterotrophic, halophilic methylotroph methylophaga thiooxydans dms010. | methylophaga thiooxydans is a mesophilic, obligately halophilic bacterium that is capable of methylotrophic growth on a range of one-carbon compounds as well as chemolithoheterotrophic growth at the expense of thiosulfate. here we present the draft genome sequence of methylophaga thiooxydans dms010 (dsm 22068(t), vkm b2586(t)), the type strain of the species, which has allowed prediction of the genes involved in one-carbon metabolism, nitrogen metabolism, and other aspects of central metabolism. | 2011 | 21478352 |
| purification, crystallization and preliminary x-ray crystallographic analysis of a methanol dehydrogenase from the marine bacterium methylophaga aminisulfidivorans mp(t). | methylophaga aminisulfidivorans mp(t) is a marine methylotrophic bacterium that utilizes c(1) compounds such as methanol as a carbon and energy source. the released electron from oxidation flows through a methanol-oxidizing system (mox) consisting of a series of electron-transfer proteins encoded by the mox operon. one of the key enzymes in the pathway is methanol dehydrogenase (mdh), which contains the prosthetic group pyrroloquinoline quinone (pqq) and converts methanol to formaldehyde in the ... | 2011 | 21505255 |
| polyphasic approach for assessing changes in an autochthonous marine bacterial community in the presence of prestige fuel oil and its biodegradation potential. | a laboratory experiment was conducted to identify key hydrocarbon degraders from a marine oil spill sample (prestige fuel oil), to ascertain their role in the degradation of different hydrocarbons, and to assess their biodegradation potential for this complex heavy oil. after a 17-month enrichment in weathered fuel, the bacterial community, initially consisting mainly of methylophaga species, underwent a major selective pressure in favor of obligate hydrocarbonoclastic microorganisms, such as al ... | 2011 | 21562979 |
| comparative analysis of two types of methanol dehydrogenase from methylophaga aminisulfidivorans mp(t) grown on methanol. | two types of methanol dehydrogenase (mdh) were obtained from a novel marine methylotrophic bacterium, methylophaga aminisulfidivorans mp(t) , grown on methanol. type i mdh consisted of two identical dimers of α (65.98 kda) and β (7.58 kda) subunits organized to form the α(2) β(2) tetramer. type ii mdh contained an additional mxaj protein (27.86 kda) and had more specific activity than type i mdh. the k(m) values of type i and ii mdh for methanol under cytochrome c(l) reduction assay system were ... | 2011 | 21656818 |
| draft genome sequence of methylophaga aminisulfidivorans mpt. | methylophaga aminisulfidivorans mp(t) is a restricted facultatively marine methylotrophic bacterium that grows on methanol, methylated amines, dimethyl sulfide, and dimethyl sulfoxide. here we present the high-quality draft genome sequence of m. aminisulfidivorans mp(t) (kctc 12909(t) = jcm 14647(t)), consisting of a chromosome (3,092,085 bp) and a plasmid (16,875 bp). | 2011 | 21685284 |
| Diversity and phylogeny of the ectoine biosynthesis genes in aerobic, moderately halophilic methylotrophic bacteria. | The genes of ectoine biosynthesis pathway were identified in six species of aerobic, slightly halophilic bacteria utilizing methane, methanol or methylamine. Two types of ectoine gene cluster organization were revealed in the methylotrophs. The gene cluster ectABC coding for diaminobutyric acid (DABA) acetyltransferase (EctA), DABA aminotransferase (EctB) and ectoine synthase (EctC) was found in methanotrophs Methylobacter marinus 7C and Methylomicrobium kenyense AMO1(T). In methanotroph Methylo ... | 2011 | 21971967 |
| emended description of the genus methylophaga janvier et al. 1985. | the genus methylophaga janvier et al. 1985 now comprises seven species with validly published names. the original description of the genus was made 26 years ago and based on only two species, namely methylophaga marina and methylophaga thalasica; as such, the description of the genus required updating to take into account the other five known species. based on literature concerning the seven species of methylophaga published over the last 26 years, i present an emended description of the genus t ... | 2011 | 21890722 |
| methylophaga lonarensis sp. nov., a novel moderately haloalkaliphilic methylotroph isolated from the soda lake sediments of a meteorite impact crater. | a novel moderately haloalkaliphilic methylotrophic bacterium (strain mpl(t)), possessing the ribulose monophosphate pathway for carbon assimilation, was isolated from two week old lonar lake sediment microcosms that were actively oxidizing methane. the isolate, which utilizes methanol, is an aerobic, gram-negative, asporogenous, motile short rod that multiplies by binary fission. it requires nahco(3) or nacl for growth and although not auxotrophic for vitamin b12, the addition of vitamin b12 enh ... | 2011 | 21890731 |
| functional diversity in the denitrifying biofilm of the methanol-fed marine denitrification system at the montreal biodome. | nitrate is a serious problem in closed-circuit public aquariums because its accumulation rapidly becomes toxic to many lifeforms. a moving bed biofilm denitrification reactor was installed at the montreal biodome to treat its 3,250-m(3) seawater system. naturally occurring microorganisms from the seawater affluent colonized the reactor carriers to form a denitrifying biofilm. here, we investigated the functional diversity of this biofilm by retrieving gene sequences related to narg, napa, nirk, ... | 2011 | 22006549 |