[frequency of clinical isolation of glucose non-fermentative gram-negative rods and their susceptibilities to antibacterial agents].a comparison was made for frequencies of isolation o glucose non-fermentative gram-negative rods ((g)nf-gnr) from clinical specimens during a period from july, 1986 to june, 1987 (the first period) and that from january, 1994 to december, 1994 (the second period). also, minimum inhibitory concentrations of principal drugs were determined against these isolates. the obtained results are summarized as follows: 1. thirty four (34) species of (g)nf-gnr were found from 35,200 clinical specimens in th ...19957474336
pcr differentiation of seventeen genospecies of the present study, strains of 17 reference acinetobacter genospecies were investigated by using the polymerase chain reaction (pcr). we used primers to amplify spacer regions between the 16s and 23s genes in the prokaryotic rrna genetic loci. when the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish all of the tested acinetobacters into 15 groups. the genospecies 5 (acinetobacter junii), 7 (acinetobacter johnsonii) and 10 prod ...19957705610
evaluation of the ability of a commercial system to identify acinetobacter genomic species.a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ...19968781881
purification of ihf-like protein from gram-negative bacteria in one chromatographic step.we describe a fast and very efficient method of purification which yields highly purified integration host factor-like proteins in one chromatographic step. ihf-like proteins from acinetobacter junii or proteus vulgaris are each an alpha beta heterodimer (subunits of 10 and 11 kda) similar to the ihf of escherichia coli when analyzed by polyacrylamide gel electrophoresis. the purified ihf are able to bind to the same ihf sites as ihf of e. coli. the results presented confirm that ihf is conserve ...19968862183
activities of beta-lactams against acinetobacter genospecies as determined by agar dilution and e-test mic methods.the agar dilution mic method was used to test activities of ticarcillin, ticarcillin-clavulanate, amoxicillin, amoxicillin-clavulanate, ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, inhibitors alone, ceftazidime, and imipenem against 237 acinetobacter genospecies. a total of 93.2% of strains were beta-lactamase positive by the chromogenic cephalosporin method. overall, ampicillin-sulbactam was the most active combination against all strains (mic at which 50% of the iso ...19979087486
structural and serological characterisation of the o-antigenic polysaccharide of the lipopolysaccharide from acinetobacter junii strain 65.a polysaccharide containing rhamnose (rha) and gal was isolated by acetic acid hydrolysis, followed by gel-permeation chromatography, from the water-soluble lipopolysaccharide (phenol/water extracted) from acinetobacter junii strain 65. the polysaccharide was characterised by means of monosaccharide analyses, smith degradation, and nmr studies, and was shown to have a linear pentasaccharide repeating unit, as depicted below. this structure was specifically recognised in western blots and enzyme ...19979151982
in vitro interaction of the ihf-like proteins acinetobacter junii and proteus vulgaris with ihf sites.the ability of the ihf-like proteins of acinetobacter junii and proteus vulgaris to interact with the h' attp and pr' ihf sites of lambda dna was investigated. ihf from a. junii and p. vulgaris was found to bind the examined ihf sites in a way similar to ihf from escherichia coli as shown by gel mobility shift dna binding assays and footprinting analysis. the three ihf proteins bound to the pr' ihf site that overlaps the-35 region of that promoter and in vitro repression of transcription by each ...19989561747
identification of the conjugative mef gene in clinical acinetobacter junii and neisseria gonorrhoeae isolates.the mef gene, originally described for gram-positive organisms and coding for an efflux pump, has been identified in clinical isolates of acinetobacter junii and neisseria gonorrhoeae. these strains could transfer the mef gene at frequencies ranging from 10(-6) to 10(-9) into one or more of the following recipients: gram-negative moraxella catarrhalis, neisseria perflava/sicca and neisseria mucosa and gram-positive enterococcus faecalis. three streptococcus pneumoniae strains could transfer the ...200010952602
resistance to antibiotics and biocides among non-fermenting gram-negative investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered gram-negative, non-fermenting bacteria.200111442564
studies on bioemulsifier production by acinetobacter strains isolated from healthy human recent years, interest has been growing in the search for novel bioemulsifiers. many bacterial genera including acinetobacter have been reported to produce bioemulsifiers. the present study aims to screen acinetobacter isolates from healthy human skin for bioemulsifier production. methods and results: acinetobacter junii sc14 produced maximum bioemulsifier in the presence of almond oil during stationary growth phase at 37 degrees c and ph 7.2. partially purified, nondialysable bioemulsifier f ...200111473593
septicemia due to acinetobacter junii.acinetobacter spp. are considered to be emerging nosocomial pathogens. acinetobacter junii is a rare cause of disease in humans and was associated mainly with bacteremia in preterm infants and pediatric oncologic patients. in this report we describe a case of catheter-related infection by a. junii in an adult oncologic patient. application of molecular methods for precise species identification of acinetobacter spp. will help to further clarify their role as human pathogens.200212089313
gram-positive mera gene in gram-negative oral and urine bacteria.clinical mercury resistant (hg(r)) gram-negative bacteria carrying gram-positive mercury reductase (mera)-like genes were characterized using dna-dna hybridization, pcr and sequencing. a pcr assay was developed which discriminated between the mera genes related to staphylococcus and those related to the bacillus/streptococcus mera genes by the difference in size of the pcr product. dna sequence analysis correlated with the pcr assay. the mera genes from acinetobacter junii, enterobacter cloacae ...200415358427
multiplex pcr for genes encoding prevalent oxa carbapenemases in acinetobacter spp.carbapenem resistance in acinetobacter baumannii is a growing public health concern and is most often mediated by oxa carbapenemases. we describe a novel multiplex polymerase chain reaction (pcr) assay able to detect and distinguish alleles encoding three subgroups of acquired oxa carbapenemases (oxa-23-like, oxa-24-like and oxa-58-like) that are scattered in acinetobacter spp., and a fourth subgroup, oxa-51-like, which appears to be intrinsic to acinetobacter baumannii. isolates belonging to tw ...200616564159
coaggregation among nonflocculating bacteria isolated from activated sludge.thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. among these strains, eight showed significant (74 to 99%) coaggregation with acinetobacter johnsonii s35 while only four strains coaggregated, to a lesser extent (43 to 65%), with acinetobacter junii s33. the extent and pattern of coaggregation as well as the aggregate size showed good correlation w ...200314532062
in vitro activity of cefepime combined with sulbactam against clinical isolates of carbapenem-resistant acinetobacter spp.the aim of this study was to assess the in vitro activity of cefepime combined with sulbactam against carbapenem-resistant clinical isolates of acinetobacter spp. the checkerboard method was used to determine whether combinations act synergistically against these strains. twenty-three acinetobacter baumannii and one acinetobacter junii found to be carbapenem resistant were included in the study. the susceptibility results for cefepime and sulbactam were interpreted according to the guidelines of ...200616987639
distribution of mef(a) in gram-positive bacteria from healthy portuguese children.we screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(a), erm(b), erm(c), erm(f), and mef(a) genes. the mef(a) genes were found in 20 (9%) of the macrolide-resistant isolates, including enterococcus spp., staphylococcus spp., and streptococcus spp. sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (orfs) described in tn1207.1, a genetic element carrying mef(a) recently described in streptococcus pneumoniae. the thre ...200212121926
phylogenetic analysis of intestinal microflora indicates a novel mycoplasma phylotype in farmed and wild salmon.all studies of the microbial community of the gastrointestinal tract of salmon to date have employed culture-based approaches, typically on pond- or tank-raised, freshwater animals. we present a phylogenetic survey of the bacterial populations present in the distal intestine of salmon from three different marine locations in europe. this was accomplished through pcr amplification, cloning, and sequencing of partial 16s rdna genes from microbial community dna isolated from the contents of the gi ...200212082453
an outbreak caused by multidrug-resistant oxa-58-positive acinetobacter baumannii in an intensive care unit in italy. 200818063199
community-acquired acinetobacter meningitis in acinetobacter meningitis in adults is an extremely rare infection of the central nervous system (cns). here we report one adult case of this rare cns infection and review the clinical data of another seven cases reported in the english language literature. in total, eight patients (six men and two women) aged between 19 and 63 years were studied. the causative pathogen in our patient was acinetobacter baumannii; in the other reported cases they were most likely acinetobacter i ...200011139162
corneal perforation due to acinetobacter junii: a case report.acinetobacter spp. is emerging as a common cause of nosocomial infections. community acquired ocular infections due to acinetobacter are rare. only one case of perforation of cornea has been reported previously, where old nomenclature was used to describe the causal agent. we report a case of corneal perforation due to acinetobacter junii for which a therapeutic penetrating keratoplasty was conducted and the patient eventually recovered.200010904196
aerators as a reservoir of acinetobacter junii: an outbreak of bacteraemia in paediatric oncology patients.tap water can play a role as a source of nosocomial pathogens, and faucet aerators have occasionally been mentioned in the literature associated with colonization or infection in hospitalized patients. in this study, we report on outbreak of bacteraemia in paediatric oncology patients caused by acinetobacter junii. environmental sampling showed the water system to be contaminated with a. junii. molecular typing using automatic laser fluorescence analysis of randomly amplified polymorphic dna (ra ...200010633050
phenotypic characterization and antibiotic resistance of acinetobacter spp. isolated from aquatic sources.a total of 99 acinetobacter isolates from sewage, freshwater aquaculture habitats, trout intestinal contents and frozen shrimps was characterized phenotypically and antibiotic susceptibility patterns determined. one group of genomic species, including ac. johnsonii, ac. lwoffi and spp. 15tu, was detected in all sample types and represented the majority of the isolates (n = 54). isolates belonging to the acb complex (ac. calcoaceticus, ac. baumannii and genomic species 3) were detected in sewage ...199910594705
comparative studies of the acinetobacter genus and the species identification method based on the reca sequences.the reca gene is indispensable for a maintaining and diversification of the bacterial genetic material. given its important role in ensuring cell viability, it is not surprising that the reca protein is both ubiquitous and well conserved among a range of prokaryotes. previously, we reported acinetobacter genomic species identification method based on pcr amplification of an internal fragment of the reca gene with subsequent restriction analysis (rflp) with hinfi and mboi enzymes. in present stud ...200212005442
phylogenetic relationship of the twenty-one dna groups of the genus acinetobacter as revealed by 16s ribosomal dna sequence analysis.the inter- and intrageneric relationships of members of the genus acinetobacter were investigated by performing a comparative sequence analysis of pcr-amplified 16s ribosomal dnas (rdnas) from 21 strains representing all of the dna groups that have been described. phylogenetic treeing confirmed that acinetobacter spp. form a coherent cluster within the gamma subdivision of the class proteobacteria that includes strains with overall levels of 16s rdna sequence similarity of more than 94%. the ana ...19979226915
a comparison of three staining methods in estimation of structures on the cell wall surface of acinetobacter junii by using electron microscope.two strains of acinetobacter junii isolated from groin and nasal cavity were studied for the presence of structures on the cell wall surface. three staining methods were used. in the first thin section were contrasted with uranyl acetate and lead citrate. in this method, only electron dense amorphous material could be seen. using only 1% uranyl acetate, thin fimbriae were observed which in few cases were collected in bundles. staining with ruthenium red showed bacteria with thick short and long ...19969127479
outbreak of septicaemia in neonates caused by acinetobacter junii investigated by amplified ribosomal dna restriction analysis (ardra) and four typing methods.septicaemia caused by acinetobacter occurred in six infants in the neonatal unit. a total of 18 acinetobacters were isolated from blood cultures, cultures of intravascular catheters, and surveillance cultures. twelve isolates from the six affected infants were identified as acinetobacter junii by the use of a novel method, amplified ribosomal dna restriction analysis (ardra). typing of the organisms using the biochemical profiles of the api 20ne system, antibiogram typing, cell envelope protein ...19979049817
metabolism of chlorinated guaiacols by a guaiacol-degrading acinetobacter junii strain.the metabolism of chlorinated guaiacols by a pure bacterial strain identified by its ability to use guaiacol as the sole carbon and energy source was studied. this strain, identified as acinetobacter junii 5ga, was unable to grow on several chlorinated guaiacols and catechols. however, strain 5ga grown on guaiacol degraded 4- and 5-chloroguaiacol and 4,5-dichloroguaiacol. under the same conditions, these cells did not degrade 6-chloroguaiacol, 4,6-dichloroguaiacol, 4,5,6-trichloroguaiacol, or te ...19938250564
clinical and epidemiological investigations of acinetobacter genomospecies 3 in a neonatal intensive care unit.a prospective study of acinetobacter isolates from a neonatal intensive care unit was performed for 24 months. fifty-six isolates were obtained from 21 patients, and another eight were obtained from environmental specimens. infection due to acinetobacter organisms was established for 16 patients, 6 with septicemia, 9 with pneumonia, and 1 with a wound infection. further investigations were performed with 38 representative isolates. twenty-nine isolates were identified as unnamed dna-dna hybridiz ...19957650188
pyrosequencing for rapid identification of carbapenem-hydrolysing oxa-type beta-lactamases in acinetobacter baumannii.carbapenem-resistant acinetobacter baumannii isolates producing carbapenem-hydrolysing oxacillinases are emerging worldwide. these enzymes are divided into four phylogenetic subgroups: oxa-23-like, oxa-51-like, oxa-24-like and oxa-58-like. a pcr-based approach linked to pyrosequencing analysis was developed to identify the genes for these beta-lactamases. carbapenem-hydrolysing oxacillinases were rapidly and unambiguously identified in a collection of carbapenem-resistant clinical isolates of a. ...200617121634
the presence of a conjugative gram-positive tn2009 in gram-negative commensal determine whether mef(a)-msr(d) and tet(m) genes are linked in representative gram-negative isolates and/or transferred together during conjugation. to molecularly characterize the acinetobacter junii element and compare the structure and sequence with the non-conjugative streptococcus pneumoniae tn2009 element.200616551693
sequencing of the rpob gene and flanking spacers for molecular identification of acinetobacter species.acinetobacter species are defined on the basis of several phenotypic characters, results of dna-dna homology, and more recently, similarities or dissimilarities in 16s rrna gene sequences. however, the 16s rrna gene is not polymorphic enough to clearly distinguish all acinetobacter species. we used an rna polymerase beta-subunit gene (rpob)-based identification scheme for the delineation of species within the genus acinetobacter, and towards that end, we determined the complete rpob gene and fla ...200616517861
inter-country transfer of gram-negative organisms carrying the vim-4 and oxa-58 carbapenem-hydrolysing enzymes. 200616476724
oxa-58 and imp-4 carbapenem-hydrolyzing beta-lactamases in an acinetobacter junii blood culture isolate from australia. 200616377723
diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil.biosurfactant production is a desirable property of hydrocarbon-degrading microorganisms (hdm). we characterized biosurfactant producing microbial populations from a long beach soil, california (usa) and a hong kong soil (china), contaminated with diesel oil. a total of 33 hydrocarbon-utilizing microorganisms were isolated from the soils. twelve isolates and three defined consortia were tested for biosurfactant production and emulsification activity. the highest reduction of surface tension was ...200516035236
selective immobilization of acinetobacter junii on the natural zeolitized tuff in municipal wastewater.the immobilization of desired bacteria onto material was usually performed in synthetic media. the aim of this study was to test the immobilization of phosphate (p)-accumulating bacteria acinetobacter junii onto natural zeolitized tuff (nz) in the raw or sterilized municipal wastewater containing the common bacteria escherichia coli and enterococcus faecalis and the performance of immobilized a. junii in the same type of wastewater. in the sterilized wastewater which contained the mixture of a. ...201121764269
studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ...200415210998
use of the midi-fame technique to characterize groundwater communities.fatty acid methyl ester (fame) profiles were identified directly from groundwater microbial communities concentrated on and extracted with polycarbonate filters. the sensitivity of this direct extraction method was determined using pure cultures of acinetobacter junii, pseudomonas putida and stenotrophomonas maltophilia. a minimum concentration of 107 cells filter-1 was required to identify the predominant fatty acids from each culture. however, at least 3.7 x 109 cells filter-1 were required to ...200010792531
identification of new delhi metallo-β-lactamase gene (ndm-1) from a clinical isolate of acinetobacter junii in delhi metallo-β-lactamase-1 (ndm-1) is a novel type of metallo-β-lactamase (mbl) responsible for bacterial resistance to β-lactam antibiotics. acinetobacter junii was previously shown to possess a mbl phenotype; however, the genes responsible for this phenotype were not identified. in this study, we reported the identification of ndm-1 gene in a clinical isolate of a. junii from a child patient in china, which was resistant to all β-lactams except aztreonam but sensitive to aminoglycosides ...201122181044
alkanindiges illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield alkane-degrading bacterium, designated gti mvab hex1(t), was isolated from chronically crude oil-contaminated soil from an oilfield in southern illinois. the isolate grew very weakly or not at all in minimal or rich media without hydrocarbons. straight-chain aliphatic hydrocarbons, such as hexadecane and heptadecane, greatly stimulated growth; shorter-chain (</=c(15)) hydrocarbons did not (with decane as the sole exception). growth was also greatly enhanced by the branched aliphatic hydrocarb ...200313130023
seven novel species of acinetobacter isolated from activated sludge.thirteen isolates of acinetobacter were obtained from activated sludge plants in victoria, australia. earlier 16s-23s rdna genomic fingerprinting and partial 16s rdna sequence data had suggested that these isolates might contain previously undescribed species. this view was confirmed here. a polyphasic taxonomic approach involving phenotypic characterization, near-complete 16s rdna sequence data and dna-dna hybridization analyses support the view that seven novel genomic species can be different ...200312892111
acinetobacter junii causes life-threatening sepsis in preterm infants.acinetobacter junii caused sepsis in six preterm infants in our neonatal unit within 48 h. each infant with clinical signs of systemic infection and activation of the acute phase response had two positive blood cultures with acinetobacter junii. the sudden onset, the short duration of the outbreak and the fact that none of the infants were colonized by a. junii suggested a common source of a. junii administered directly into the blood. the only feature shared by all six affected newborns was an ...199910447139
intergeneric coaggregations among oligotropha carboxidovorans and acinetobacter species present in activated sludge.the coaggregation traits of two pairs of sewage sludge bacteria were tested and characterized. oligotropha carboxidovorans s23 coaggregated with two strains of the genus acinetobacter viz. acinetobacter junii s33 (56%) and acinetobacter johnsonii s35 (99%). coaggregates of o. carboxidovorans s23 and a. junii s33 were small (20-40 microm), weak and susceptible to edta and a commercial protease (actinase e). actinase/periodate pretreatment of the partners prior to coaggregation revealed that inter ...200312855163
acinetobacter junii causes refractory peritonitis in a patient on automated peritoneal dialysis. 200717179525
[study on the structure and function of a stable methane-oxidizing mixed microbial consortium].from an agricultural sample taken in chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. the mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. more than 99% of phenol at an initial concentration of 600mg/l could be degraded by the mixed microbial consortium after 11 h of cultivation. the productivity of 1, 2-epoxypropane could be increas ...200717436634
quorum sensing in metal tolerance of acinetobacter junii bb1a is associated with biofilm production.acinetobacter junii strain bb1a, a novel metal-tolerant bacterium, produced biofilm in the presence of added ions such as ni(2+), aso(2)(-), cd(2+) and hg(2+) on surfaces such as glass and polystyrene. generation of a metal-sensitive and adhesion-deficient mutant by transposition of tn5-mob in the a. junii genome has putatively confirmed the association of metal tolerance with the production of biofilm. the requirement of a critical cell density for biofilm formation and presence of acyl-homoser ...200818397291
outbreak of infection by carbapenem-resistant acinetobacter baumannii producing the carbapenemase oxa-58 in belgium.carbapenem-resistant acinetobacter baumannii isolates were obtained from 17 patients between september 2004 and august 2005 at the academisch ziekenhuis vrije universiteit brussel, brussels, belgium. these multidrug-resistant isolates, which belonged to a single clone, remained susceptible to colistin and tigecycline only and produced the carbapenem-hydrolyzing oxacillinase oxa-58. this study highlights the importance of the intercountry spread of this beta-lactamase-mediated resistance mechanis ...200616957031
characterization of the carbapenem-hydrolyzing oxacillinase oxa-58 in an acinetobacter genospecies 3 clinical isolate.based on imipenem resistance in an acinetobacter genospecies 3 clinical isolate, we were able to identify, for the first time in this genomic species, a plasmid-encoded bla(oxa-58) gene that was 100% homologous to the same gene in acinetobacter baumannii.200818505859
clinical characteristics of patients with acinetobacter junii infection.acinetobacter junii is a human pathogen but a. junii infection is rarely reported. this study aimed to delineate the characteristics of a. junii infection.200919424558
endemic and epidemic acinetobacter species in a university hospital: an 8-year survey.the prevalence of the currently known acinetobacter species and related trends of antimicrobial resistance in a dutch university hospital were studied. between 1999 and 2006, acinetobacter isolates from clinical samples were collected prospectively. isolates were analyzed by amplified fragment length polymorphism fingerprinting. for species identification, a profile similarity cutoff level of 50% was used, and for strain identification, a cutoff level of 90% was used. susceptibility for antimicr ...200919794057
a single clone of acinetobacter baumannii, st22, is responsible for high antimicrobial resistance rates of acinetobacter spp. isolates that cause bacteremia and urinary tract infections in korea.we investigated the characteristics of a total of 96 acinetobacter spp. isolates that were shown to cause bacteremia and urinary tract infections (utis) from 10 university hospitals located in various regions of korea from november 2006 to august 2007. the antimicrobial susceptibilities of these isolates were determined using a broth microdilution method, and the species were identified using molecular identification. in addition, we performed multilocus sequence typing for acinetobacter baumann ...201020370437
dark fermentative hydrogen production from enzymatic hydrolysate of xylan and pretreated rice straw by clostridium butyricum cgs5.xylan and rice straw were used to produce h(2) via a two-stage approach combining feedstock pretreatment/hydrolysis and dark h(2) fermentation. acinetobacter junii f6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. the hydrolysates were converted to h(2) by clostridium butyricum cgs5 via dark fermentation. investigation of kinetics of xylanase on xylan and naoh-pretreated rice straw shows nu(max) values of 8.6 and 3.6g/l/h, and k(m) val ...201020385486
blood stream infections caused by acinetobacter ursingii in an obstetrics ward.the genus acinetobacter is an important causative pathogen of nosocomial infections in the healthcare setting. the objectives of this study were to determine the species of causative pathogens and the sources of acinetobacter blood stream infections that occurred in 2 immunocompetent pregnant women admitted to an obstetrics ward within a 2-month period. phenotypic identification of the two isolates from blood stream infections was inconsistent among the id test, the microscan walkaway and the vi ...201020969979
arsenite oxidizing multiple metal resistant bacteria isolated from industrial effluent: their potential use in wastewater treatment.arsenite oxidizing bacteria, isolated from industrial wastewater, showed high resistance against arsenite (40 mm) and other heavy metals (10 mm pb; 8 mm cd; 6 mm cr; 10 mm cu and 26.6 mm as(5+)). bacterial isolates were characterized, on the basis of morphological, biochemical and 16s rrna ribotyping, as bacillus cereus (1.1s) and acinetobacter junii (1.3s). the optimum temperature and ph for the growth of both strains were found to be 37 °c and 7. both the strains showed maximum growth after 24 ...201627339314
on the zinc sorption by the serbian natural clinoptilolite and the disinfecting ability and phosphate affinity of the exhausted sorbent.the serbian natural zeolite is moderately effective in removing the zinc(ii) ions from aqueous solutions. at 298 k the sorption capacity varies from 13 to 26% for the initial zn(ii) solution concentration of 100 and 600 mg zndm(-3), respectively. the sorption isotherm at 298-338 k is best represented by the langmuir model and the sorption kinetics by the pseudo-second-order model. the sorption involves a combination of film diffusion, intra-particle diffusion, and a chemical cation-exchange betw ...201020940085
carriage of oxa-58 but not of oxa-51 beta-lactamase gene correlates with carbapenem resistance in acinetobacter baumannii. 200616997843
exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.this study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (mfcs) using activated sludge for wine-containing wastewater treatment. phylogenetic tree analysis on 16s rrna gene fragments indicated that the predominant strains on anodic biofilm in acclimatized mfcs were gamma-proteobacteria aeromonas punctata niu-p9, pseudomonas plecoglossicida niu-y3, pseudomonas koreensis niu-x8, acinetobacter junii niu-y8, stenotropho ...201120859654
interspecies dissemination of a novel class 1 integron carrying blaimp-19 among acinetobacter species in analyse the dissemination of metallo-β-lactamase genes and mobile genetic elements among acinetobacter spp.201121862476
identification of acinetobacter species: is bruker biotyper maldi-tof mass spectrometry a good alternative to molecular techniques?acinetobacter spp. has become a leading cause of nosocomial infection in recent years. phenotypic similarities between the species in the genus have made it difficult to identify them clearly using routine diagnostic methods. consequently, more relevant species have been grouped together as acinetobacter calcoaceticus-acinetobacter baumannii complex (a. baumannii, a. calcoaceticus, acinetobacter genospecies 3 and a. genospecies 13tu). however, there are other species that may also have clinical ...201222266021
acinetobacter indicus sp. nov., isolated from hexachlorocyclohexane (hch) dumpsite.taxonomic position of a gram-negative, non-motile, oxidase negative and catalase positive strain a648t, isolated from hexachlorocyclohexane (hch) dumpsite located in lucknow, india was ascertained using a polyphasic approach. a comparative analysis of partial sequence of the rpob gene and 16s rrna gene sequence revealed that strain a648t belongs to the genus acinetobacter. dna-dna relatedness values between strain a648t and other closely related members namely acinetobacter radioresistens dsm 69 ...201222247213
reclassification of acinetobacter grimontii carr et al. 2003 as a later synonym of acinetobacter junii bouvet and grimont 1986.using tdna-pcr, the type strain ccm 7198t (<--cip 107470t <--17a04t) of acinetobacter grimontii was found to be indistinguishable from acinetobacter junii strains. therefore, the phenotypic properties, amplified fragment length polymorphism (aflp) patterns and 16s rrna and rpob gene sequences of the type strain of a. grimontii (ccm 7198t) were determined. we found that the strain used l-arginine and l-glutamate, in contrast to the original description and in accordance with the phenotypic proper ...200818398198
the effect of mineral carrier composition on phosphate-accumulating bacteria immobilization.the goal of this study was to determine the dynamics and yield of immobilization of the phosphate-accumulating bacterium acinetobacter junii on mineral carriers. as mineral carriers natural clinoptilolite tuff from turkey (t) and serbia (s) and natural bentonite (ter), in original and magnesium (mg)-exchanged form were used. the key feature which determined the extent of immobilization of a. junii was the type of carrier; the immobilization yield decreased in order t>ter>s. the number of immobil ...200919153002
three-dimensional human skin equivalent as a tool to study acinetobacter baumannii colonization.acinetobacter baumannii can colonize body surfaces of hospitalized patients. from these sites, invasion into the host and spread to other patients and the hospital environment may occur. the eradication of the organism from the patient's skin is an important infection control strategy during epidemic and endemic episodes. in this study, a three-dimensional (3d), air-exposed human epidermal skin equivalent was exploited to study acinetobacter skin colonization. we characterized the adherence of a ...201222290957
arsenic methylation by micro-organisms isolated from sheepskin bedding materials.sudden infant death syndrome (sids) has been associated with the volatilization of arsenic, antimony or phosphorus compounds from infants' bedding material by micro-organisms, the so-called 'toxic gas hypothesis'. the volatilization of arsenic by aerobic micro-organisms isolated from new sheepskin bedding material, as well as on material used by a healthy infant and by an infant who perished of sids, was examined. three fungi were isolated from a piece of sheepskin bedding material on which an i ...200312856955
presence of oxa-23-producing isolates of acinetobacter baumannii in wastewater from hospitals in southern brazil.the aim of the study was to evaluate the dissemination of multiresistant isolates of acinetobacter baumannii carrying resistance genes, by samples of wastewater from hospitals in porto alegre, rio grande do sul, brazil. we obtained 303 bacterial isolates from the wastewater of three hospitals in porto alegre, rio grande do sul. for each isolate, we determined the profile of susceptibility to antimicrobials and the presence of the genes bla(oxa-23), bla(oxa-24), bla(oxa-51), bla(oxa-58), bla(spm- ...201121254809
microcalorimetric investigation of the toxic action of pyrene on the growth of pah-degrading bacteria acinetobacter junii.a multi-channel thermal activity microcalorimeter was used to determine the pyrene-induced toxic effect on two polycyclic aromatic hydrocarbon (pah)-degrading bacteria acinetobacter junii (a. junii) and bacillus subtilis (b. subtilis). power-time curves were analyzed and calorimetric parameters including growth rate constant (k), half inhibitory concentration (ic50) and total thermal effect (qt) were obtained. a. junii and b. subtilis were completely inhibited when the concentration of pyrene re ...201020390914
requirement of acinetobacter junii for magnesium, calcium and potassium ions.the goal of this study was to determine the concentrations of mg, ca and k ions required for the formation of metabolically active population of phosphate (p)-accumulating bacterium acinetobacter junii. the availability of mg, ca and k originating from natural minerals in the conditions of severe shortage of these cations was tested. in the case of shortage of mg, ca and k ions in wastewater the p removal was absent due to the decay of a. junii. in the cases of mg or k shortage in wastewater the ...201020547327
antimicrobial activity of commercial zeolite a on acinetobacter junii and saccharomyces cerevisiae.the influence of three samples of commercially produced zeolite a (named a, m and r) in water medium on the bacterium acinetobacter junii and yeast saccharomyces cerevisiae was investigated. these microorganisms were used in the bioassay and are not specifically related to the use of zeolite a. all zeolite samples showed the negative influence on the survival and physiological status of a. junii and s. cerevisiae. the ec(50) values for the inhibition of cfu of a. junii were 0.328, 0.138 and 0.13 ...201020705392
to invade or not to invade: two approaches to a prokaryotic predatory life cycle.bdellovibrio and like organisms (balos) are a group of gram-negative bacterial predators that are defined as having a periplasmic life cycle, whereby the predator enters into the periplasm of a prey cell. recently, a predator of caulobacter crescentus with a novel epibiotic life cycle was identified as a new species - bdellovibrio exovorus. therefore, this raises the question as to what determines the type of life cycle of a predator. six bacterial strains susceptible to predation by b. exovorus ...201323586752
a new insight to adsorption and accumulation of high lead concentration by exopolymer and whole cells of lead-resistant bacterium acinetobacter junii l. pb1 isolated from coal mine dump.a lead-resistant bacterial strain was isolated from coal mine dump and identified as acinetobacter junii pb1 on basis of 16s rrna (ribosomal ribonucleic acid) gene sequencing. the minimum inhibitory concentration of lead for the strain was 16,000 mg l(-1) and it showed antibiotic and multi metal resistance. in aqueous culture, at an initial lead (pb(ii)) concentration of 100 and 500 mg l(-1), lead adsorption and accumulation by the isolate was 100 and 60%, at ph 7 at 30 °c after 48 and 120 h, re ...201728283975
unravelling the interactions among microbial populations found in activated sludge during biofilm formation.microorganisms colonize surfaces and develop biofilms through interactions that are not yet thoroughly understood, with important implications for water and wastewater systems. this study investigated the interactions between n-acyl homoserine lactone (ahl)-producing bacteria, yeasts and protists, and their contribution to biofilm development. sixty-one bacterial strains were isolated from activated sludge and screened for ahl production, with aeromonas sp. found to be the dominant ahl producer. ...201627306553
acinetobacter plantarum sp. nov. isolated from wheat seedlings plant.strain thg-sqm11(t), a gram-negative, aerobic, non-motile, coccus-shaped bacterium, was isolated from wheat seedlings plant in p. r. china. strain thg-sqm11(t) was closely related to members of the genus acinetobacter and showed the highest 16s rrna sequence similarities with acinetobacter junii (97.9 %) and acinetobacter kookii (96.1 %). dna-dna hybridization showed 41.3 ± 2.4 % dna reassociation with a. junii kctc 12416(t). chemotaxonomic data revealed that strain thg-sqm11(t) possesses ubiqui ...201626869166
urban riverine environment is a source of multidrug-resistant and esbl-producing clinically important acinetobacter spp.some acinetobacter species have emerged as very important opportunistic pathogens in humans. we investigated acinetobacter spp. from the polluted urban riverine environment in croatia in regard to species affiliation, antibiotic resistance pattern, and resistance mechanisms. considerable number of isolates produced acquired extended-spectrum β-lactamase(s) (esbls), ctx-m-15 solely or with tem-116. by southern blot hybridization, bla tem-116 was identified on plasmids ca. 10, 3, and 1.2 kb in aci ...201626490931
strategies for decreasing contamination of homemade nasal saline irrigation solutions.saline nasal irrigations (sni) are an important adjunct in the treatment of rhinosinusitis, and many patients prepare and store these solutions in their homes without an awareness of the potential for contamination. the objectives of this study were to determine if such contamination occurs and the effect of preparation methods on contamination.201626290494
nitrogen removal characteristics of a heterotrophic nitrifier acinetobacter junii yb and its potential application for the treatment of high-strength nitrogenous wastewater.acinetobacter junii yb was found to exhibit efficient heterotrophic nitrification and aerobic denitrification ability, with the maximum ammonium, nitrite and nitrate removal rate of 8.82, 8.45 and 7.98 mg/l/h, respectively. meanwhile, ammonium was found to be removed preferentially in the process of simultaneous nitrification and denitrification in mixed n-sources. the successful pcr amplification of hao, napa and nirs genes further provided additional evidence of heterotrophic nitrification and ...201526141282
the impact of horizontal gene transfer on targeting the internal transcribed spacer region (its) to identify acinetobacter junii strains.despite electrophoretic patterns of its pcr amplicons often suggesting only a single its sequence variant is present in strains of acinetobacter junii, sequence data shows differences in its copies between and among them. this paper set out to explain why these its variants arise, and whether their presence compromises the reliability of the its targeted methods currently available for typing ac. junii strains.201525801684
the characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, acinetobacter junii yb.a novel heterotrophic nitrifying bacterium was isolated from activated sludge and was identified as acinetobacter junii yb. the strain exhibited efficient heterotrophic nitrification-aerobic denitrification ability at a broad range of ammonium loads and had the capability to utilize hydroxylamine, nitrite and nitrate as a sole nitrogen source. based on the nitrogen removal and enzyme assay, the nitrogen removal pathway was speculated to be achieved through heterotrophic nitrification coupled wit ...201425171329
first report of blandm and blaoxa-58 coexistence in acinetobacter junii. 201424951805
cloning, expression and characterization of a lipase encoding gene from human oral metagenome.the human oral metagenomic dna cloned into plasmid puc19 was used to construct a dna library in escherichia coli. functional screening of 40,000 metagenomic clones led to identification of a clone lip2 that exhibited halo on tributyrin agar plate. sequence analysis of lip2 insert dna revealed a 939 bp orf (omlip1) which showed homology to lipase 1 of acinetobacter junii sh205. the omlip1 orf was cloned and expressed in e. coli bl21 (de3) using pet expression system. the recombinant enzyme was pu ...201424891735
genome sequencing and annotation of acinetobacter junii strain mtcc 11364.the genus acinetobacter consists of 31 validly published species ubiquitously distributed in nature and primarily associated with nosocomial infection. we report the 3.5 mb draft genome of the acinetobacter junii strain mtcc 11364. the genome has a g + c content of 38.0% and includes 3 rrna genes (5s, 23s, 16s) and 64 aminoacyl-trna synthetase genes.201326484056
bacteremia caused by an acinetobacter junii strain harboring class 1 integron and diverse dna mobile elements.infections caused by acinetobacter junii are rarely reported. however, some outbreaks of septicemia in neonates and pediatric oncology patients, as well as meningitis, peritonitis, and ocular infection have been described. since it is highly infrequent to find the molecular characterization of a. junii strains in literature, in this study we described the molecular characterization of a. junii isolates recovered from blood samples of a renal transplant patient.201424820473
rhamnolipids produced by indigenous acinetobacter junii from petroleum reservoir and its potential in enhanced oil recovery.biosurfactant producers are crucial for incremental oil production in microbial enhanced oil recovery (meor) processes. the isolation of biosurfactant-producing bacteria from oil reservoirs is important because they are considered suitable for the extreme conditions of the reservoir. in this work, a novel biosurfactant-producing strain acinetobacter junii bd was isolated from a reservoir to reduce surface tension and emulsify crude oil. the biosurfactants produced by the strain were purified and ...201627872613
presence of new delhi metallo-β-lactamase gene (ndm-1) in a clinical isolate of acinetobacter junii in argentina. 201627257491
bloodstream infection caused by acinetobacter junii in a patient with acute lymphoblastic leukaemia after allogenic haematopoietic cell transplantation.acinetobacter junii is a rare human pathogen associated with bacteraemia in neonates and paediatric oncology patients. we present a case of a. junii causing bacteraemia in an adult transplant patient with leukaemia. the correct identification of acinetobacter species can highlight the clinical significance of the different species of this genus.201021109630
characterization of carbapenem-resistant acinetobacter calcoaceticus-baumannii complex isolates from nosocomial bloodstream infections in southern iran.acinetobacter baumannii is an important opportunistic bacterial pathogen responsible for serious infections in hospitalized patients. from a total of 78 consecutive non-repetitive acinetobacter spp. isolates from patients with blood infections, 61 were carbapenem resistant, which were positive for blaoxa-51-like (96.7%), blaoxa-23-like (77 %), blaoxa-58-like (8.1%) and blaoxa-40-like genes (32.8%) by multiplex pcr. the isolates were identified as a. baumannii (n = 59) and acinetobacter nosocomia ...201626747061
identification of opportunistic pathogenic bacteria in drinking water samples of different rural health centers and their clinical impacts on drinking water quality monitoring programs have been established in order to prevent or to reduce the risk of contracting water-related infections. a survey was performed on groundwater-derived drinking water from 13 different hospitals in the mahabubnagar district. a total of 55 bacterial strains were isolated which belonged to both gram-positive and gram-negative bacteria. all the taxa were identified based on the 16s rrna gene sequence analysis based on which they are phylogenet ...201323862144
detection of ndm-1 carbapenemase-producing acinetobacter calcoaceticus and acinetobacter junii in environmental samples from livestock farms. 201525349061
biodegradation of triton x-100 and its primary metabolites by a bacterial community isolated from activated sludge.a set of studies was carried using a continuous flow biodegradation unit in order to isolate a microbial community capable of efficient and complete utilization of octylphenol ethoxylates from activated sludge. increasing concentrations of triton x-100 (in the range of 1-1000 mg/l) were applied over a time period of 35 days in order to select microorganisms, which exhibit high tolerance towards this surfactant. the fate of the surfactant and its primary degradation products was assessed by hplc/ ...201323770380
enhancing the hexavalent chromium bioremediation potential of acinetobacter junii vitsukmw2 using statistical design experiments.the cr(vi) removal capability of acinetobacter junii vitsukmw2 isolated from the sukinda chromite mine site was evaluated and enhanced using statistical design techniques. the removal capacity was evaluated at different ph values (5-11) and temperatures (30-40 degrees c) and with various carbon and nitrogen sources. plackett- burman design was used to select the operational parameters for bioremediation of cr(vi). three parameters (molasses, yeast extract, and cr(vi) concentration) were chosen f ...201223221541
batch and continuous flow studies of adsorptive removal of cr(vi) by adapted bacterial consortia immobilized in alginate beads.the adsorptive removal of cr(vi) by alginate beads containing cr(vi)-adapted acinetobacter junii, escherichia coli and bacillus subtilis in batch and continuous packed bed column reactors was investigated. under optimized conditions (ph 3.0; contact time, 180 min; 30 °c; initial cr(vi) concentration of 100 mg/l), 65.86 mg/g adsorption capacity was recorded in the batch study. when an adsorbent dosage of 1g/l, a flow rate of 5 ml/min, a bed height of 20 cm, an initial cr(vi) concentration of 300 ...201323201524
flocculating property of extracellular polymeric substances produced by a biofilm-forming bacterium acinetobacter junii bb1a.extracellular polymeric substances (eps) produced by a biofilm-producing bacterium acinetobacter junii bb1a were characterized. purified eps on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). infrared spectrometry revealed the presence of hydroxyl, carboxyl, and amide groups. the average molecular weight of the polysaccharide (ps) fraction of eps was ~2×10(5). gas liquid chromatography analysis ...201222968590
bacteremia caused by acinetobacter junii at a medical center in taiwan, 2000-2010.we investigated the clinical characteristics and outcomes of 43 patients with acinetobacter junii bacteremia at a 2,500-bed tertiary care center in northern taiwan. these organisms were confirmed to the species level by an array assay and 16s rrna gene sequence analysis. the antimicrobial susceptibilities of the 43 a. junii isolates to 13 agents were determined using the agar dilution method. susceptibility testing for tigecycline was determined using the broth microdilution method. most of the ...201222562410
production of a plasmid-encoded oxa-72 β-lactamase associated with resistance to carbapenems in a clinical isolate acinetobacter junii. 201221982835
evaluation of the bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of acinetobacter species.matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) (bruker biotyper) was able to accurately identify 98.6% (142/144) of acinetobacter baumannii isolates, 72.4% (63/87) of a. nosocomialis isolates, and 97.6% (41/42) of a. pittii isolates. all acinetobacter junii, a. ursingii, a. johnsonii, and a. radioresistens isolates (n = 28) could also be identified correctly by bruker biotyper.201424899038
structural and immunological studies of an exopolysaccharide from acinetobacter junii bb1a.a water-soluble heteropolysaccharide (ps) was isolated from extracellular polymeric substances (eps) produced by a novel metal tolerant bacterium, acinetobacter junii bb1a. sugar analysis showed that the ps was composed of mannose, galactose and arabinose in a molar ratio of nearly 3:1:1. structural characterization of the ps was carried out using methylation analysis, periodate oxidation, smith degradation and 1d/2d nmr experiments. methylation analysis revealed that the ps was consisted of 2,4 ...201424299764
first report of the emergence of new delhi metallo-β-lactamase-1 producing acinetobacter junii in nanjing, china. 201323867689
copper susceptibility in acinetobacter junii bb1a is related to the production of extracellular polymeric substances.acinetobacter junii bb1a cells, grown in different media, were differentially inhibited in the presence of the copper. the minimum inhibitory concentration of cu(2+) was influenced by the nutrient status of the media. the production of extracellular polymeric substances (eps) was stimulated by the copper present in the growth medium. the nature of the eps was anionic showing non-newtonian pseudoplastic behaviour. the thermal behaviour of the eps was studied by differential scanning calorimetry. ...201323756604
resistance of bioparticles formed of phosphate-accumulating bacteria and zeolite to harsh environmental conditions.extreme environmental conditions, such as ph fluctuations, high concentrations of toxicants or grazing of protozoa, can potentially be found in wastewater treatment systems. this study was carried out to provide specific evidence on how 'bioparticles' can resist these conditions. the term 'bioparticle' is used to describe a particle comprising natural zeolitized tuff with a developed biofilm of the phosphate-accumulating bacterial species, acinetobacter junii, on the surface. the bacteria in the ...201323706006
a case of community-acquired acinetobacter junii-johnsonii cellulitis.acinetobacter skin and soft tissue infection outside of the traumatic wound setting are rare occurrences. the majority of cases occur in the presence of significant comorbilities and by acinetobacter baumanii. herein a case is reported of community-onset, health-care-associated, non-traumatic cellulitis caused by acinetobacter, species junii-johnsonii with bacteremia. this is the first reported case of acinetobacter junii-johnsonii skin and soft tissue infection. hemorrhagic bullae might be one ...201223242290
comparative analysis of fecal microflora of healthy full-term indian infants born with different methods of delivery (vaginal vs cesarean): acinetobacter sp. prevalence in vaginally born this study fecal microflora of human infants born through vaginal delivery (vb) and through cesarean section (cb) were investigated using culture-independent 16s rdna cloning and sequencing approach. the results obtained clearly revealed that fecal microbiota of vb infants distinctly differ from those in their counterpart cb infants. the intestinal microbiota of infants delivered by cesarean section appears to be more diverse, in terms of bacteria species, than the microbiota of vaginally del ...201223151789
worldwide dissemination of acquired carbapenem-hydrolysing class d β-lactamases in acinetobacter spp. other than acinetobacter baumannii.the aim of this study was to identify acquired oxa-type carbapenemases in acinetobacter spp. other than acinetobacter baumannii. from a total of 453 carbapenem-susceptible and -resistant acinetobacter isolates collected worldwide, 23 were positive for blaoxa genes by multiplex pcr. these isolates were identified as acinetobacter pittii (n=18), acinetobacter nosocomialis (n=2), acinetobacter junii (n=1) and acinetobacter genomic species 14tu/13bj (n=2). the blaoxa genes and associated insertion s ...201424612983
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