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[occurrence of species from acinetobacter genus in clinical material and other sources].the analysis regarded 304 strains of acinetobacter genus isolated from various diagnostic materials, objects from hospital environment and from non-hospital sources (soil, water, various animals). applying api zone system, five species were isolated: acinetobacter juni, (18.42%), acinetobacter baumanii (70.39%), acinetobacter haemolyticus (5.59%), acinetobacter lwoffii (4.6%) and acinetobacter johnsonii (0.99%). most frequently isolated species were present in purulent materials and in samples f ...19921297033
a numerical taxonomic study of non-motile non-fermentative gram-negative bacteria from foods.a numerical taxonomic study using 155 unit characters was performed on 63 strains of gram-negative non-motile non-fermentative bacteria isolated from proteinaceous foods. similar bacteria from other sources and several pseudomonas strains from meat were included for reference purposes. three clusters were observed at 76% s which contained all the food strains. cluster 1 was composed entirely of acinetobacter strains including 17 isolated from foods that were provisionally identified with acineto ...19883209519
pcr differentiation of seventeen genospecies of acinetobacter.in the present study, strains of 17 reference acinetobacter genospecies were investigated by using the polymerase chain reaction (pcr). we used primers to amplify spacer regions between the 16s and 23s genes in the prokaryotic rrna genetic loci. when the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish all of the tested acinetobacters into 15 groups. the genospecies 5 (acinetobacter junii), 7 (acinetobacter johnsonii) and 10 prod ...19957705610
generation of a proton motive force by the excretion of metal-phosphate in the polyphosphate-accumulating acinetobacter johnsonii strain 210a.the strictly aerobic, polyphosphate-accumulating acinetobacter johnsonii strain 210a degrades its polyphosphate when oxidative phosphorylation is impaired. the endproducts of this degradation, divalent metal ions and inorganic phosphate, are excreted as a neutral metal-phosphate (mehpo4) chelate via the electrogenic mehpo4/h+ symport system of the organism. the coupled excretion of mehpo4 and h+ in a. johnsonii 210a can generate a proton motive force. in membrane vesicles and deenergized cells, ...19947961934
airborne environmental endotoxin: a cross-validation of sampling and analysis techniques.a standard method for measurement of airborne environmental endotoxin was developed and field tested in a fiberglass insulation-manufacturing facility. this method involved sampling with a capillary-pore membrane filter, extraction in buffer using a sonication bath, and analysis by the kinetic-limulus assay with resistant-parallel-line estimation (klare). cross-validation of the extraction and assay method was performed by comparison with methanolysis of samples followed by 3-hydroxy fatty acid ...19948161191
energetics of alanine, lysine, and proline transport in cytoplasmic membranes of the polyphosphate-accumulating acinetobacter johnsonii strain 210a.amino acid transport in right-side-out membrane vesicles of acinetobacter johnsonii 210a was studied. l-alanine, l-lysine, and l-proline were actively transported when a proton motive force of -76 mv was generated by the oxidation of glucose via the membrane-bound glucose dehydrogenase. kinetic analysis of amino acid uptake at concentrations of up to 80 microm revealed the presence of a single transport system for each of these amino acids with a kt of less than 4 microm. the mode of energy coup ...19948169217
characterization of restriction endonuclease ajoi from acinetobacter johnsonii.a new type ii restriction endonuclease, named ajoi, was detected in acinetobacter johnsonii. the enzyme ajoi, an isoschizomer of psti, recognized the hexanucleotide sequence [5'-ctgca/g-3'], with a cleavage site generating fragments of dna with protruding cohesive 3' termini.19948181713
substrate specificity of the two phosphate transport systems of acinetobacter johnsonii 210a in relation to phosphate speciation in its aquatic environment.in natural waters and domestic waste waters in which divalent metal ions are present in excess of pi, h2po4-, hpo4(2-), and mehpo4 prevail at ph values physiological for acinetobacter johnsonii 210a (ph 5.5-8.0). in view of the ability of this organism to extensively accumulate pi and divalent cations in cytoplasmic polyphosphate granules, the substrate specificity of its two pi transport systems was studied. the constitutive, proton motive force-driven pi carrier, previously shown to be depende ...19948206923
vascular catheter-related bloodstream infection due to acinetobacter johnsonii (formerly acinetobacter calcoaceticus var. lwoffi): report of 13 cases.although acinetobacter baumannii (formerly acinetobacter calcoaceticus var. anitratus) is known to be an important nosocomial pathogen, the clinical impact of other acinetobacter species is not fully understood. acinetobacter johnsonii (formerly a subset of a. calcoaceticus var. lwoffii) is considered a commensal on human skin, and infections due to this organism have not been reported previously. during a study period of 18 months, however, 13 patients at 6 hospitals developed catheter-related ...19938268343
properties of polyphosphatase of acinetobacter johnsonii 210a.polyphosphatase, an enzyme which hydrolyses highly polymeric polyphosphates to pi, was purified 77-fold from acinetobacter johnsonii 210a by q-sepharose, hydroxylapatite and mono-q column chromatography. the native molecular mass estimated by gel filtration and native gel electrophoresis was 55 kda. sds-polyacrylamide gel electrophoresis indicated that polyphosphatase of acinetobacter johnsonii 210a is a monomer. the enzyme was specific for highly polymeric polyphosphates and showed no activity ...19938274005
mechanism and energetics of the secondary phosphate transport system of acinetobacter johnsonii 210a.the mechanism and energetics of the secondary pi transport system of a. johnsonii were studied in membrane vesicles and proteoliposomes in which the transport protein was functionally reconstituted. pi uptake is strictly dependent on the presence of divalent cations, like mg2+, ca2+, mn2+, or co2+. these cations form a mehpo4 complex with up to 87% of the pi present in the incubation mixture, suggesting that divalent cations and pi are co-transported via a metal-phosphate chelate. metal-phosphat ...19938366084
characterization of two phosphate transport systems in acinetobacter johnsonii 210a.the transport of p(i) was characterized in acinetobacter johnsonii 210a, which is able to accumulate an excessive amount of phosphate as polyphosphate (polyp) under aerobic conditions. p(i) is taken up against a concentration gradient by energy-dependent, carrier-mediated processes. a. johnsonii 210a, grown under p(i) limitation, contains two uptake systems with kt values of 0.7 +/- 0.2 microm and 9 +/- 1 microm. p(i) uptake via the high-affinity component is drastically reduced by n,n'-dicycloh ...19938380151
effect of carbon:nitrogen ratio on kinetics of phenol biodegradation by acinetobacter johnsonii in saturated sand.in polluted soil or ground water, inorganic nutrients such as nitrogen may be limiting, so that monod kinetics for carbon limitation may not describe microbial growth and contaminant biodegradation rates. to test this hypothesis we measured 14co2 evolved by a pure culture of acinetobacter johnsonii degrading 120 micrograms 14c-phenol per ml in saturated sand with molar carbon:nitrogen (cn) ratios ranging from 1.5 to 560. we fit kinetics models to the data using non-linear least squares regressio ...19958580643
autophosphorylation of a bacterial protein at tyrosine.autophosphorylation at tyrosine is a common process in eukaryotic kinases, which is generally modulated by regulatory ligands and affects the properties of these enzymes. we report that this type of modification occurs also in bacteria, namely in an 81 kda protein from acinetobacter johnsonii. this protein is phosphorylated at the expense of atp exclusively at tyrosine residues. it is located in the inner-membrane fraction of cells and can be totally solubilized by detergents. it has been purifi ...19968683591
evaluation of the ability of a commercial system to identify acinetobacter genomic species.a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ...19968781881
activities of beta-lactams against acinetobacter genospecies as determined by agar dilution and e-test mic methods.the agar dilution mic method was used to test activities of ticarcillin, ticarcillin-clavulanate, amoxicillin, amoxicillin-clavulanate, ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, inhibitors alone, ceftazidime, and imipenem against 237 acinetobacter genospecies. a total of 93.2% of strains were beta-lactamase positive by the chromogenic cephalosporin method. overall, ampicillin-sulbactam was the most active combination against all strains (mic at which 50% of the iso ...19979087486
phylogenetic relationship of the twenty-one dna groups of the genus acinetobacter as revealed by 16s ribosomal dna sequence analysis.the inter- and intrageneric relationships of members of the genus acinetobacter were investigated by performing a comparative sequence analysis of pcr-amplified 16s ribosomal dnas (rdnas) from 21 strains representing all of the dna groups that have been described. phylogenetic treeing confirmed that acinetobacter spp. form a coherent cluster within the gamma subdivision of the class proteobacteria that includes strains with overall levels of 16s rdna sequence similarity of more than 94%. the ana ...19979226915
characterization of a bacterial gene encoding an autophosphorylating protein tyrosine kinase.acinetobacter johnsonii harbors a protein tyrosine kinase activity that is able to catalyze autophosphorylation, like a number of eukaryotic tyrosine kinases. a biochemical and genetic analysis of this enzyme was performed. maximum phosphorylation in vitro was obtained by incubating the kinase for 2 min at ph 7.0 in the presence of 5 mm magnesium chloride. in contrast to eukaryotic enzymes, no inhibitory effect of genistein and no phosphorylation of synthetic substrates such as poly (glu80 tyr20 ...19979434192
phosphate transport in prokaryotes: molecules, mediators and mechanisms.bacteria have evolved sophisticated pi transport systems which combine high affinity with coupling to metabolic energy. this review discusses the current evidence concerning the physiological, biochemical, and molecular properties of these pi transport systems in prokaryotes. major developments of the past years will be presented with emphasis on three kinds of issues. first, work on pi transport in escherichia coli and the polyphosphate-accumulating acinetobacter johnsonii has assigned a novel ...19979442271
functional characterization of the low-molecular-mass phosphotyrosine-protein phosphatase of acinetobacter johnsonii.the ptp gene of acinetobacter johnsonii was previously reported to encode a low-molecular-mass protein, ptp, whose amino acid sequence, predicted from the theoretical analysis of the nucleotide sequence of the gene, exhibits a high degree of similarity with those of different eukaryotic and prokaryotic phosphotyrosine-protein phophatases. we have now overexpressed the ptp gene in escherichia coli cells, purified the ptp protein to homogeneity by a single-step chromatographic procedure, and analy ...19989571056
inhibition of anaerobic phosphate release by nitric oxide in activated sludge.activated sludge not containing significant numbers of denitrifying, polyphosphate [poly(p)]-accumulating bacteria was grown in a fill-and-draw system and exposed to alternating anaerobic and aerobic periods. during the aerobic period, poly(p) accumulated up to 100 mg of p x g of (dry) weight. when portions of the sludge were incubated anaerobically in the presence of acetate, 80 to 90% of the intracellular poly(p) was degraded and released as orthophosphate. degradation of poly(p) was mainly ca ...19989687452
phylogenetic structures of the genus acinetobacter based on gyrb sequences: comparison with the grouping by dna-dna hybridization.the phylogenetic relationships of 49 acinetobacter strains, 46 of which have previously been classified into 18 genomic species by dna-dna hybridization studies, were investigated using the nucleotide sequence of gyrb, the structural gene for the dna gyrase b subunit. the phylogenetic tree showed linkages between genomic species 1 (acinetobacter calcoaceticus), 2 (acinetobacter baumannii), 3 and tu13; genomic species 6, bj15, bj16 and bj17; genomic species 5, bj13 (synonym of tu14) and bj14; gen ...199910028249
on the binding of atp to the autophosphorylating protein, ptk, of the bacterium acinetobacter johnsonii.the autophosphorylating protein, ptk, of the bacterium acinetobacter johnsonii was overproduced, purified to homogeneity and assayed for atp binding by using the nucleotide analog 5'-p-fluorosulfonylbenzoyl adenosine. the atp binding site of this bacterial autophosphorylating protein was found to be different from that generally used by eukaryotic protein kinases. it consists of two amino acid sequences that closely resemble the walker motifs a and b. this observation was confirmed by site-direc ...199910069388
protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide.in eukaryotes, tyrosine protein phosphorylation has been studied extensively, while in bacteria, it is considered rare and is poorly defined. we demonstrate that escherichia coli possesses a gene, etk, encoding an inner membrane protein that catalyses tyrosine autophosphorylation and phosphorylation of a synthetic co-polymer poly(glu:tyr). this protein tyrosine kinase (ptk) was termed ep85 or etk. all the e.coli strains examined possessed etk; however, only a subset of pathogenic strains express ...199910369665
the effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of acinetobacter johnsonii.a strain of acinetobacter with potential for bioremediation of heavy metal-contaminated waters was isolated from a wastewater-treatment plant operating an enhanced biological phosphate removal process. nmr and extractive methods showed that polyphosphate accumulated aerobically was degraded under anaerobic conditions both in the presence and absence of cadmium or uranium (0.2-0.5 mm). nmr showed that free phosphate was formed at the expense of polyphosphate, and an extractive technique indicated ...199910439410
biochemical properties of the protein tyrosine kinase of the bacterium acinetobacter johnsonii.the biochemical properties of the autophosphorylating protein tyrosine kinase of acinetobacter johnsonii were analyzed in vitro. the study shows that the optimal ph value for the phosphorylation reaction is approximately 7. the enzyme activity is stimulated by magnesium and, to a lesser extent, by manganese ions, whereas calcium ions have no effect. the phosphorylation process is rapid reaching a maximum in < 2 min, and the enzyme is modified at multiple sites. interestingly, the bacterial enzym ...199910690649
psychrobacters and related bacteria in freshwater fish.three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ...200010716558
in vitro atp regeneration from polyphosphate and amp by polyphosphate:amp phosphotransferase and adenylate kinase from acinetobacter johnsonii 210a.in vitro enzyme-based atp regeneration systems are important for improving yields of atp-dependent enzymatic reactions for preparative organic synthesis and biocatalysis. several enzymatic atp regeneration systems have been described but have some disadvantages. we report here on the use of polyphosphate:amp phosphotransferase (ppt) from acinetobacter johnsonii strain 210a in an atp regeneration system based on the use of polyphosphate (polyp) and amp as substrates. we have examined the substrat ...200010788379
investigation of an outbreak of gram-negative bacteremia among hematology-oncology outpatients.to identify risk factors associated with an outbreak of gram-negative bacteremia (gnb).200011001264
purification and properties of pyrophosphatase of acinetobacter johnsonii 210a and its involvement in the degradation of polyphosphate.inorganic pyrophosphatase (e.c. 3.6.1.1) of acinetobacter johnsonii 210a was purified 200-fold to apparent homogeneity. the enzyme catalyzed the hydrolysis of inorganic pyrophosphate and triphosphate to orthophosphate. no activity was observed with other polyphosphates and a wide variety of organic phosphate esters. the molecular mass of the enzyme was estimated to be 141 kda by gelfiltration. sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated a subunit composition of six identi ...199911068824
inorganic polyphosphate kinase and adenylate kinase participate in the polyphosphate:amp phosphotransferase activity of escherichia coli.polyphosphate kinase (ppk), responsible for the processive synthesis of inorganic polyphosphate (polyp) from atp in escherichia coli, can transfer in reverse the terminal phosphate residue of polyp to adp to yield atp. polyp also serves as a donor in a polyp:amp phosphotransferase (pap) activity observed in extracts of acinetobacter johnsonii and myxococcus xanthus. we have found that overexpression of the gene encoding ppk results in a large enhancement of pap activity in e. coli. the pap activ ...200011106368
community-acquired acinetobacter meningitis in adults.community-acquired acinetobacter meningitis in adults is an extremely rare infection of the central nervous system (cns). here we report one adult case of this rare cns infection and review the clinical data of another seven cases reported in the english language literature. in total, eight patients (six men and two women) aged between 19 and 63 years were studied. the causative pathogen in our patient was acinetobacter baumannii; in the other reported cases they were most likely acinetobacter i ...200011139162
phosphate uptake and release by acinetobacter johnsonii in continuous culture and coupling of phosphate release to heavy metal accumulation.a strain of polyphosphate-synthesizing, phosphate-releasing acinetobacter johnsonii was isolated from a wastewater treatment plant operating enhanced biological phosphate removal (ebpr) and was used to remove la(3+) from solution via precipitation of cell-bound lapo(4). the effect of repeated aerobic-anaerobic cycles on the carbon and phosphate metabolism of the organism was studied in attempts to promote increased phosphate flux using a three-stage, continuous bioreactor comprising aerobic, ana ...200111571615
isolation and characterization of a protein-tyrosine kinase and a phosphotyrosine-protein phosphatase from klebsiella pneumoniae.two proteins of klebsiella pneumoniae, termed yor5 and yco6, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. first, protein yco6 was overproduced from its specific gene and purified to homogeneity by affinity chromatography. upon incubation in the presence of radioactive adenosine triphosphate, it was found to effectively autophosphorylate. two-dimensional analysis of its phosphoamino acid content revealed that it was modified exclusivel ...200211742763
comparative studies of the acinetobacter genus and the species identification method based on the reca sequences.the reca gene is indispensable for a maintaining and diversification of the bacterial genetic material. given its important role in ensuring cell viability, it is not surprising that the reca protein is both ubiquitous and well conserved among a range of prokaryotes. previously, we reported acinetobacter genomic species identification method based on pcr amplification of an internal fragment of the reca gene with subsequent restriction analysis (rflp) with hinfi and mboi enzymes. in present stud ...200212005442
quantifying the impact of wastewater micronutrient composition on in situ growth activity of acinetobacter spp.batch growth studies with pure cultures of acinetobacter johnsoniit and acinetobacter johnsonii strain 210 on various media formulations were used to examine the effects of micronutrient composition on the growth rate of microbial populations in wastewater treatment systems. on nutrient rich luria-bertani medium, both strains of a. johnsonii grew with a doubling time of approximately 30 min. on a defined, minimal medium with acetate as the sole carbon source, the doubling time of a. johnsoniit w ...200212216664
a novel beta-diketone-cleaving enzyme from acinetobacter johnsonii: acetylacetone 2,3-oxygenase.a novel fe+zn containing oxygenase from acinetobacter johnsonii catalyses 2,3-cleavage of acetylacetone to acetate and methylglyoxal has been purified. the stoichiometry of reactants and products conforms to a classical dioxygenase. the pure protein is a homotetramer of 64kd with variable amounts of fe(2+) and zn(2+). activity of the enzyme is more closely related to the fe(2+) content than to the amount of protein. a purification of acetylacetone 2,3-oxygenase, some of its physical properties, ...200212237107
acetylacetone-cleaving enzyme dke1: a novel c-c-bond-cleaving enzyme from acinetobacter johnsonii.the toxicity of acetylacetone has been demonstrated in various studies. little is known, however, about metabolic pathways for its detoxification or mineralization. data presented here describe for the first time the microbial degradation of acetylacetone and the characterization of a novel enzyme that initiates the metabolic pathway. from an acinetobacter johnsonii strain that grew with acetylacetone as the sole carbon source, an inducible acetylacetone-cleaving enzyme was purified to homogenei ...200312379146
chloroxylenol- and triclosan-tolerant bacteria from industrial sources.potential development of bacterial tolerance to biocides in the industrial environment is examined in this study. bacteria tolerant to the phenolic-type agent para-chloro-meta-xylenol (pcmx) and the bis-phenol 2,4,4'-trichloro-2'-hydroxydiphenylether (triclosan) were isolated from industrial sources and identified. minimum inhibitory concentrations (mics) were determined and compared with those of culture collection (standard) strains. of around 100 isolates originally obtained, most were natura ...200212407457
intergeneric coaggregations among oligotropha carboxidovorans and acinetobacter species present in activated sludge.the coaggregation traits of two pairs of sewage sludge bacteria were tested and characterized. oligotropha carboxidovorans s23 coaggregated with two strains of the genus acinetobacter viz. acinetobacter junii s33 (56%) and acinetobacter johnsonii s35 (99%). coaggregates of o. carboxidovorans s23 and a. junii s33 were small (20-40 microm), weak and susceptible to edta and a commercial protease (actinase e). actinase/periodate pretreatment of the partners prior to coaggregation revealed that inter ...200312855163
pair-dependent co-aggregation behavior of non-flocculating sludge bacteria.two strains of non-flocculating sewage sludge bacteria (xanthomonas sp. s53 and microbacterium esteraromaticum s51) showed 91% and 77% co-aggregation, respectively, with acinetobacter johnsonii s35 using a spectrophometric assay. the co-aggregates in case of xanthomonas sp. s53 and a. johnsonii s35 were above 100 microm and stable against edta (2 mm) and a commercial protease (0.2 mg ml(-1)). protease/periodate pretreatment of the partners did not affect this co-aggregation. on the other hand, c ...200312889835
coaggregation among nonflocculating bacteria isolated from activated sludge.thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. among these strains, eight showed significant (74 to 99%) coaggregation with acinetobacter johnsonii s35 while only four strains coaggregated, to a lesser extent (43 to 65%), with acinetobacter junii s33. the extent and pattern of coaggregation as well as the aggregate size showed good correlation w ...200314532062
diketone cleaving enzyme dke1 production by acinetobacter johnsonii--optimization of fermentation conditions.the main objective of this work was the optimization of the production of the novel dioxygenase diketone cleaving enzyme (dke1) from acinetobacter johnsonii. acetylacetone was used as an inducer for enzyme production. in the first step, the growth medium was optimized by using screening designs for finding the optimal carbon and nitrogen source. in the second step, a genetic algorithm was used to optimize the concentrations of all medium components. after six generations the stopping criterion w ...200414687973
bloodstream infections in febrile neutropenic patients: bacterial spectrum and antimicrobial susceptibility pattern.bacterial infections are the major cause of morbidity and mortality among neutropenic patients. prompt administration of empiric antimicrobial therapy for febrile neutropenic patients is considered vital. before putting neutropenic patients on empiric antimicrobial regimens, it is essential to be aware of the spectrum of locally prevalent pathogens and their susceptibility pattern.200415125174
stability of bacterial coaggregates in extreme environments.the stability of coaggregates formed by the interaction of acinetobacter johnsonii s35 and oligotropha carboxidovorans s23, both of which are nonflocculating sludge bacteria, was evaluated by a spectrophotometric assay of aggregation index and phase contrast microscopy. exposure of coaggregates to conditions of various ph (3-11) or temperature (10-5 degrees c) did not cause significant deflocculation and high aggregation index (85-93%) was still maintained as compared to 93.3% under normal condi ...200415244326
polyphosphate synthetic activity of polyphosphate:amp phosphotransferase in acinetobacter johnsonii 210a.polyphosphate:amp phosphotransferase (pap) has been identified as an enzyme that catalyzes the phosphorylation of amp with inorganic polyphosphates [poly(p)] as phosphate donors. we found that the purified pap of acinetobacter johnsonii 210a has poly(p) synthetic activity. the pap catalyzes the dephosphorylation of adp and processively synthesizes poly(p) of 200 to 700 residues. comparatively lower concentrations of mgcl(2) (20 mm) were required to obtain optimum poly(p) synthetic activity, wher ...200415262957
electronic substituent effects on the cleavage specificity of a non-heme fe(2+)-dependent beta-diketone dioxygenase and their mechanistic implications.acinetobacter johnsonii acetylacetone dioxygenase (dke1) is a non-heme fe(ii)-dependent dioxygenase that cleaves c-c bonds in various beta-dicarbonyl compounds capable of undergoing enolization to a cis-beta-keto enol structure. results from 18o labeling experiments and quantitative structure-reactivity relationship analysis of electronic substituent effects on the substrate cleavage specificity of dke1 are used to distinguish between two principle chemical mechanisms of reaction: one involving ...200415453718
inactivation of gram-negative and gram-positive bacteria in red cell concentrates using inactine pen110 chemistry.the risk of transfusion-transmitted bacterial infections as a result of the presence of bacteria in blood is one of the major concerns in transfusion medicine. the purpose of this study was to investigate whether bacteria inoculated into red blood cell concentrates can be inactivated by the inactine pen110 pathogen-reduction process. four bacterial species were chosen for the study: anaerobic gram-positive clostridium perfringens and propionibacterium acnes, known to be transfusion-transmitted; ...200415569065
polyphosphate:amp phosphotransferase as a polyphosphate-dependent nucleoside monophosphate kinase in acinetobacter johnsonii 210a.we have cloned the gene for polyphosphate:amp phosphotransferase (pap), the enzyme that catalyzes phosphorylation of amp to adp at the expense of polyphosphate [poly(p)] in acinetobacter johnsonii 210a. a genomic dna library was constructed in escherichia coli, and crude lysates of about 6,000 clones were screened for pap activity. pap activity was evaluated by measuring atp produced by the coupled reactions of pap and purified e. coli poly(p) kinases (ppks). in this coupled reaction, pap produc ...200515716459
emergent mechanistic diversity of enzyme-catalysed beta-diketone cleavage.the enzymatic cleavage of c-c bonds in beta-diketones is, comparatively, a little studied biochemical process, but one that has important relevance to human metabolism, bioremediation and preparative biocatalysis. in recent studies, four types of enzymes have come to light that cleave c-c bonds in the beta-diketone functionality using different chemical mechanisms. oph [oxidized poly(vinyl alcohol) hydrolase from pseudomonas sp. strain vm15c], which cleaves nonane-4,6-dione to butyrate and penta ...200515934927
reaction coordinate analysis for beta-diketone cleavage by the non-heme fe2+-dependent dioxygenase dke1.acetylacetone dioxygenase from acinetobacter johnsonii (dke1) utilizes a non-heme fe2+ cofactor to promote dioxygen-dependent conversion of 2,4-pentanedione (pd) into methylglyoxal and acetate. an oxidative carbon-carbon bond cleavage by dke1 is triggered from a c-3 peroxidate intermediate that performs an intramolecular nucleophilic attack on the adjacent carbonyl group. but how does dke1 bring about the initial reduction of dioxygen? to answer this question, we report here a reaction coordinat ...200516131208
coaggregation between acinetobacter johnsonii s35 and microbacterium esteraromaticum strains isolated from sewage activated sludge.the extent and nature of intergeneric coaggregations among non-flocculating sludge bacteria were studied through examination of the coaggregation abilities of acinetobacter johnsonii s35 with two other strains of non-flocculating sludge bacteria (microbacterium esteraromaticum s38 and m. esteraromaticum s51). at first, the effect of electrolyte concentration as well as the addition of edta and proteases on coaggregation were studied. changes in electrolyte concentration had little effect on the ...200316233476
effect of surfactants on stability of acinetobacter johnsonii s35 and oligotropha carboxidovorans s23 coaggregates.the effect of anionic (sodium dodecyl sulphate or sds) and cationic (cetyltrimethylammonium bromide or ctab) surfactants on the stability of binary bacterial coaggregates comprising acinetobacter johnsonii s35 and oligotropha carboxidovorans s23 (both sewage sludge isolates) was studied and compared with that on the complex sewage sludge flocs. both sds and ctab enhanced the bacterial coaggregation at their lower concentrations of 0.2 and 0.07 mg ml(-1), respectively. however, complete defloccul ...200516329879
diverse uv-b resistance of culturable bacterial community from high-altitude wetland water.isolation of most ultraviolet b (uv-b)-resistant culturable bacteria that occur in the habitat of laguna azul, a high-altitude wetland [4554 m above sea level (asl)] from the northwestern argentinean andes, was carried out by culture-based methods. water from this environment was exposed to uv-b radiation under laboratory conditions during 36 h, at an irradiance of 4.94 w/m2. it was found that the total number of bacteria in water samples decreased; however, most of the community survived long-t ...200616604419
plasticity of the gene functions for dna replication in the t4-like phages.we have completely sequenced and annotated the genomes of several relatives of the bacteriophage t4, including three coliphages (rb43, rb49 and rb69), three aeromonas salmonicida phages (44rr2.8t, 25 and 31) and one aeromonas hydrophila phage (aeh1). in addition, we have partially sequenced and annotated the t4-like genomes of coliphage rb16 (a close relative of rb43), a. salmonicida phage 65, acinetobacter johnsonii phage 133 and vibrio natriegens phage nt-1. each of these phage genomes exhibit ...200616828113
diverse responses to uv-b radiation and repair mechanisms of bacteria isolated from high-altitude aquatic environments.acinetobacter johnsonii a2 isolated from the natural community of laguna azul (andean mountains at 4,560 m above sea level), serratia marcescens mf42, pseudomonas sp. strain mf8 isolated from the planktonic community, and cytophaga sp. strain mf7 isolated from the benthic community from laguna pozuelos (andean puna at 3,600 m above sea level) were subjected to uv-b (3,931 j m-2) irradiation. in addition, a marine pseudomonas putida strain, 2idinh, and a second acinetobacter johnsonii strain, atc ...200617056692
bacterial growth kinetics estimation by fluorescence in situ hybridization and spectrofluorometric quantification.the aim of this study was to develop a specific and rapid method to identify and quantify relevant bacterial populations in mixed biomass by spectrofluorometric quantification (sq) of whole cells hybridized with fluorescently labelled oligonucleotide probes targeting mature 16s ribosomal rna (rrna). probe targeting the precursor of rrna synthesis was also employed because it was being suggested as more indicative of the activity state of the micro-organisms.200717576227
novel coaggregating microbial consortium: testing strength for field applications.acinetobacter johnsonii s35 has an ability to coaggregate with oligotropha carboxidovorans s23 when grown with favorable carbon substrate and nutrient-rich growth media. however, nutrient-deficient situations or poorly accessible carbon substrates in waste streams could trigger changes in the cell surface properties and thus modify the coaggregation behavior. thus, in the present study, we test the stability of these novel interactions under various stresses that might transpire in the waste str ...200817765538
emergence and widespread dissemination of oxa-23, -24/40 and -58 carbapenemases among acinetobacter spp. in asia-pacific nations: report from the sentry surveillance program.the aim of this study was to evaluate the occurrence and dissemination of acquired carbapenem-hydrolysing class d beta-lactamase (class d carbapenemase)- and metallo-beta-lactamase (mbl)-encoding genes among acinetobacter spp. isolates recovered from medical centres in the asia-pacific (apac) region.200918957398
biochemical characterization and mutational analysis of the mononuclear non-haem fe2+ site in dke1, a cupin-type dioxygenase from acinetobacter johnsonii.beta-diketone-cleaving enzyme dke1 is a homotetrameric fe2+-dependent dioxygenase from acinetobacter johnsonii. the dke1protomer adopts a single-domain beta-barrel fold characteristic of the cupin superfamily of proteins and features a mononuclear non-haem fe2+ centre where a triad of histidine residues, his-62, his-64 and his-104, co-ordinate the catalytic metal. to provide structure-function relationships for the peculiar metal site of dke1 in relation to the more widespread 2-his-1-glu/asp bi ...200918973472
triclosan-tolerant bacteria: changes in susceptibility to antibiotics.there is no clear consensus regarding the effect of biocide tolerance on antibiotic susceptibility. in this work, triclosan-tolerant strains of escherichia coli, staphylococcus aureus and acinetobacter johnsonii were compared with sensitive strains in order to ascertain their susceptibility to a range of antibiotics. the minimum inhibitory concentrations of triclosan were measured using broth- and agar-dilution techniques. antibiotic susceptibilities were determined using the british society for ...200919246121
biodegradation of malathion by acinetobacter johnsonii ma19 and optimization of cometabolism substrates.to enhance the removal efficiency of malathion in the wastewater from organophosphate pesticide mill, a bacterium, acinetobacter johnsonii ma19, that could degrade malathion with cometabolism was isolated from malathion-polluted soil samples using enrichment culture techniques. four kinds of additional compounds, sodium succinate, sodium acetate, glucose, and fructose were tested to choose a favorite carbon source for the cometabolism of strain ma19. the results showed that sodium succinate and ...200919402403
endemic and epidemic acinetobacter species in a university hospital: an 8-year survey.the prevalence of the currently known acinetobacter species and related trends of antimicrobial resistance in a dutch university hospital were studied. between 1999 and 2006, acinetobacter isolates from clinical samples were collected prospectively. isolates were analyzed by amplified fragment length polymorphism fingerprinting. for species identification, a profile similarity cutoff level of 50% was used, and for strain identification, a cutoff level of 90% was used. susceptibility for antimicr ...200919794057
comparative study on characteristics of azo dye decolorization by indigenous decolorizers.this study provides a first attempt to explore indigenous strains with excellent decolorization capability from the most biodiverse region in taiwan for dye-bearing wastewater treatment. bacterial isolates were obtained via serial selections under selection pressure of the fungicide nystatin and model textile dye(s). according to profiles of protein expression and pcr-augmented 16s rrna gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to ty ...201019944603
characterization of a novel trimethoprim resistance gene, dfra28, in class 1 integron of an oligotrophic acinetobacter johnsonii strain, mb52, isolated from river mahananda, india.the present work has validated the capability of using diluted luria broth (10(-3)) and its amendment with antibiotics to quantify proportions of antibiotic-resistant oligotrophic bacteria contained in river water. the assay conditions for assessing fractions of antibiotic-resistant oligotrophic bacteria, especially the type and concentration of antibiotic exposure by determining ld(50) concentration for each antibiotic using sensitive oligotrophic strain, have been standardized. the presence of ...201020192821
growth and photosynthetic efficiency promotion of sugar beet (beta vulgaris l.) by endophytic bacteria.very little is known about the physiological interactions between plants and endophytic bacteria. we investigated the impact of three endophytic bacteria, bacillus pumilus 2-1, chryseobacterium indologene 2-2, and acinetobacter johnsonii 3-1, on the photosynthetic capacity and growth of sugar beet. endophyte-free plants were obtained first and infected with the bacteria. measurements of total chlorophyll content revealed very significant differences between endophyte-free beet plants and some in ...201020405213
recurrent isolation of extremotolerant bacteria from the clean room where phoenix spacecraft components were assembled.the microbial burden of the phoenix spacecraft assembly environment was assessed in a systematic manner via several cultivation-based techniques and a suite of nasa-certified, cultivation-independent biomolecule-based detection assays. extremotolerant bacteria that could potentially survive conditions experienced en route to mars or on the planet's surface were isolated with a series of cultivation-based assays that promoted the growth of a variety of organisms, including spore formers, mesophil ...201020446872
detection of airborne bacteria in a german turkey house by cultivation-based and molecular methods.today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. in this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the conce ...201020720091
acinetobacter kyonggiensis sp. nov., a β-glucosidase-producing bacterium, isolated from sewage treatment plant.a gram-negative, non-motile bacterium, designated ksl5401-037(t), was isolated from a sewage treatment plant in gwangju in the republic of korea and was characterized using a polyphasic taxonomic approach. comparative 16s rrna gene sequence analysis showed that strain ksl5401-037(t) belonged to the genus acinetobacter in the family moraxellaceae of the gammaproteobacteria (brisou and prevot, 1954). according to a 16s rrna gene sequence analysis, it was closely related to acinetobacter johnsonii ...201021221930
[progress in reasearch on acinetobacter johnsonii.] 201121457673
Genetic Diversity of Endophytic Diazotrophs of the Wild Rice, Oryza alta and Identification of the New Diazotroph, Acinetobacter oryzae sp. nov.Thirty-three endophytic diazotrophs were isolated from surface-sterilized leaves, stem, and roots of wild rice Oryza alta. The SDS-PAGE profile of total protein and insertion sequence-based polymerase chain reaction (IS-PCR) fingerprinting grouped the isolates into four clusters (I-IV). The 16S rRNA gene sequence homology of the representative strains B21, B31, B1, and B23 of clusters I, II, III, and IV were assigned to Pseudomonas oleovorans (99.2% similarity), Burkholderia fungorum (99.4% simi ...201122105517
interspecies dissemination of a novel class 1 integron carrying blaimp-19 among acinetobacter species in japan.to analyse the dissemination of metallo-β-lactamase genes and mobile genetic elements among acinetobacter spp.201121862476
genome shuffling improves production of the low-temperature alkalophilic lipase by acinetobacter johnsonii.the production of a low-temperature alkalophilic lipase from acinetobacter johnsonii was improved using genome shuffling. the starting populations, obtained by uv irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. the optimal conditions for protoplast formation and regeneration were 0.15 mg lysozyme/ml for 45 min at 37°c. the protoplasts were inactivated under uv for 20 min or heated at 60°c for 60 min and a fusant probability of ~98% was observed. the po ...201221972140
involvement of acinetobacter sp. in the floc-formation in activated sludge process.the coaggregation behavior of acinetobacter johnsonii s35 isolate with sewage bacteria was assessed by a spectrophotometric assay using different samples from a municipal wastewater treatment plant and a community plant. a. johnsonii s35 coaggregated well with other free bacteria and microflocs at the mixing ratios of 0.2:1-0.6:1 of a. johnsonii s35 and sewage samples. in addition, the size of coaggregates became larger (100μm or more) under the same conditions. a. johnsonii s35 cells were highl ...201122001846
identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from acinetobacter haemolyticus, acinetobacter johnsonii, and acinetobacter calcoaceticus.clin microbiol infect abstract: carbapenem resistance is increasingly being reported among acinetobacter species, and results mostly from the expression of acquired carbapenem-hydrolysing oxacillinases (chdls). several acinetobacter species intrinsically possess chromosomal chdl genes: acinetobacter baumannii (bla(oxa-51) ), acinetobacter radioresistens (bla(oxa-23) ), and acinetobacter lwoffii (bla(oxa-134) ). we aimed to identify the progenitors of novel chdl-encoding genes for identification ...201122128805
mucosal and salivary microbiota associated with recurrent aphthous stomatitis.recurrent aphthous stomatitis (ras) is a common oral mucosal disorder of unclear etiopathogenesis. although recent studies of the oral microbiota by high-throughput sequencing of 16s rrna genes have suggested that imbalances in the oral microbiota may contribute to the etiopathogenesis of ras, no specific bacterial species associated with ras have been identified. the present study aimed to characterize the microbiota in the oral mucosa and saliva of ras patients in comparison with control subje ...201627036492
metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia.mixed microbial cultures, in which bacteria and fungi interact, have been proposed as an efficient way to deconstruct plant waste. the characterization of specific microbial consortia could be the starting point for novel biotechnological applications related to the efficient conversion of lignocellulose to cello-oligosaccharides, plastics and/or biofuels. here, the diversity, composition and predicted functional profiles of novel bacterial-fungal consortia are reported, on the basis of replicat ...201424955113
data on the standardization of a cyclohexanone-responsive expression system for gram-negative bacteria.engineering of robust microbial cell factories requires the use of dedicated genetic tools somewhat different from those traditionally used for laboratory-adapted microorganisms. we have edited and formatted the chnr/p chnb regulatory node of acinetobacter johnsonii to ease the targeted engineering of ectopic gene expression in gram-negative bacteria. the proposed compositional standard was thoroughly verified with a monomeric and superfolder green fluorescent protein (msf•gfp) in escherichia co ...201626870759
the genetic analysis of an acinetobacter johnsonii clinical strain evidenced the presence of horizontal genetic transfer.acinetobacter johnsonii rarely causes human infections. while most a. johnsonii isolates are susceptible to virtually all antibiotics, strains harboring a variety of β-lactamases have recently been described. an a. johnsonii aj2199 clinical strain recovered from a hospital in buenos aires produces per-2 and oxa-58. we decided to delve into its genome by obtaining the whole genome sequence of the aj2199 strain. genome comparison studies on aj2199 revealed 240 unique genes and a close relation to ...201627548264
the complex genetic context of blaper-1 flanked by miniature inverted-repeat transposable elements in acinetobacter johnsonii.on a large plasmid of acinetobacter johnsonii strain xbb1 from hospital sewage, blaper-1 and iscr1 were found in a complex tn402-like integron carrying an arr3-aaca4 cassette array. the integron was truncated by the same 439-bp miniature inverted-repeat transposable element (mite) at both ends. blaper-1 and its complex surroundings might have been mobilized by the mitest into an orf of unknown function, evidenced by the presence of the characteristic 5-bp direct target repeats. the same 439-bp m ...201424587208
lettuce and fruits as a source of multidrug resistant acinetobacter spp.the role of ready-to-eat products as a reservoir of pathogenic species of acinetobacter remains unclear. the objective of the present study was to evaluate the presence of acinetobacter species in lettuces and fruits marketed in portugal, and their susceptibility to antimicrobials. acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. among 253 isolates tha ...201728213015
genome-sequence analysis of acinetobacter johnsonii mb44 reveals potential nematode-virulent factors.acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. however, a newly identified isolate of this species from a frost-plant-tissue sample, namely, a. johnsonii mb44, showed significant nematicidal activity against the model organism caenorhabditis elegans. to expand our understanding of this bacterial species, we generated a draft genome sequence of mb44 and analyzed its genomic features related to nematicidal attribute ...201627429894
antimicrobial resistance and resistance genes in aerobic bacteria isolated from pork at slaughter.the aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the people's republic of china. the organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). high levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to ...201627052863
[frequency and antimicrobial resistance of acinetobacter species in a university hospital of buenos aires city].two-hundred acinetobacter isolates belonging to 200 patients admitted to hospital de clínicas josé de san martín during the period march 2013-june 2014 were analyzed. the identification was performed by mass spectrometry and was confirmed by molecular methods. susceptibility to antimicrobials was studied by the vitek-2 system. a 94% correlation of both identification methods was found. multidrug resistant acinetobacter baumannii was the predominant genomic species (92.6%) in hospital-acquired in ...201525576416
extremophilic acinetobacter strains from high-altitude lakes in argentinean puna: remarkable uv-b resistance and efficient dna damage repair.high-altitude andean lakes (haal) of the south american andes are almost unexplored ecosystems of shallow lakes. the haal are recognized by a remarkably high uv exposure, strong changes in temperature and salinity, and a high content of toxic elements, especially arsenic. being exposed to remarkably extreme conditions, they have been classified as model systems for the study of life on other planets. particularly, acinetobacter strains isolated from the haal were studied for their survival compe ...201222644565
acinetobacter kookii sp. nov., isolated from soil.two gram-stain-negative, non-fermentative bacterial strains, designated 11-0202(t) and 11-0607, were isolated from soil in south korea, and four others, luh 13522, luh 8638, luh 10268 and luh 10288, were isolated from a beet field in germany, soil in the netherlands, and sediment of integrated fish farms in malaysia and thailand, respectively. based on 16s rrna, rpob and gyrb gene sequences, they are considered to represent a novel species of the genus acinetobacter. their 16s rrna gene sequence ...201323950148
multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp.bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ...201424288029
enrichment of acinetobacter spp. from food samples.relatively little is known about the role of foods in the chain of transmission of acinetobacters and the occurrence of different acinetobacter spp. in foods. currently, there is no standard procedure to recover acinetobacters from food in order to gain insight into the food-related ecology and epidemiology of acinetobacters. this study aimed to assess whether enrichment in dijkshoorn enrichment medium followed by plating in chromagar™ acinetobacter medium is a useful method for the isolation of ...201626742623
surface water isolates of hemolytic and non-hemolytic acinetobacter with multiple drug and heavy metal resistance ability.acinetobacter in surface waters are a major concern because of their rapid development of resistance to a wide range of antimicrobials and their ability to persist in these waters for a very long time. four surface water isolates of acinetobacter having both multidrug- and multimetal-resistant ability were isolated and identified through biochemical tests and 16s rdna sequencing. based on these analyses, two hemolytic isolates were affiliated with acinetobacter haemolyticus with an accession num ...201424642427
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
characterization of novel diesel-degrading strains acinetobacter haemolyticus mj01 and acinetobacter johnsonii mj4 isolated from oil-contaminated soil.the diesel-degrading strains, designated as mj01 and mj4, were isolated from oil-contaminated soil in daejeon (south korea) and were taxonomically characterized using a polyphasic approach and their diesel oil degradation abilities were analyzed. the isolates mj01 and mj4 were identified as acinetobacter haemolyticus and acinetobacter johnsonii, respectively, based on their 16s rdna gene sequences, dna-dna relatedness, fatty acid profiles and various physiological characteristics. strains mj01 a ...201222806027
isolation and characterization of a virulent bacteriophage infecting acinetobacter johnsonii from activated sludge.a double-stranded dna phage named ajo1, infecting acinetobacter johnsonii, which plays an important role in wastewater treatment, was isolated from activated sludge in a full-scale municipal wastewater treatment plant. based on morphological taxonomy, ajo1, with an icosahedral head 55 ± 2 nm in diameter and a non-contractile tail 8 ± 2 nm in length, was classified as a member of the podoviridae family. bacterial infection characteristics were as follows: no polyvalent infectivity, optimal multip ...201728213104
induction of diverse bioactive secondary metabolites from the mangrove endophytic fungus trichoderma sp. (strain 307) by co-cultivation with acinetobacter johnsonii (strain b2).two new sesquiterpenes, microsphaeropsisin b (1) and c (2), and two new de-o-methyllasiodiplodins, (3r, 7r)-7-hydroxy-de-o-methyllasiodiplodin (4) and (3r)-5-oxo-de-o-methyllasiodiplodin (5), together with one new natural product (6) and twelve known compounds (3, 7-17), were isolated from the co-cultivation of mangrove endophytic fungus trichoderma sp. 307 and aquatic pathogenic bacterium acinetobacter johnsonii b2. their structures, including absolute configurations, were elucidated by extensi ...201728208607
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
consumption of a high-fat diet alters the seminal fluid and gut microbiomes in male mice.our prior work showed that a novel microbiome resides in the seminal vesicles of wild-type and oestrogen receptor α (esr1) knock-out mice and is impacted by the presence of functional esr1 genes. the seminal fluid microbiome (sfm) may influence the health and reproductive status of the male, along with that of his partner and offspring. a high-fat diet (hfd) alters metabolites and other factors within seminal fluid and might affect the sfm. adult (~15 weeks old) male mice were placed for 4 weeks ...201627569192
characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from france and luxembourg.in this study, antibiotic resistance class 3 integrons in gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. two samples of hospital effluent from france and luxembourg and one sample of sludge from a wastewater treatment plant in france were collected in 2010 and 2011. bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative pcr. integron gene cassette arrays and their genetic environments ...201627499434
unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy.three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps.201726987764
draft genome sequence of acinetobacter johnsonii mb44, exhibiting nematicidal activity against caenorhabditis elegans.acinetobacter johnsonii mb44 was isolated from a frost-plant-tissue sample, which showed noteworthy nematicidal activity against the model organism caenorhabditis elegans. here, we report the 3.4 mb draft genome of a. johnsonii mb44, which will help in understanding the molecular mechanism of its ability to infect nematodes.201626893438
the bacterial microbiome in chronic rhinosinusitis: richness, diversity, postoperative changes, and patient outcomes.the bacterial microbiome in chronic rhinosinusitis (crs) remains poorly understood. microorganisms are believed to be important contributors to the inflammatory response seen in these patients.201626867528
cspe is overproduced by temperature downshift in the acinetobacter johnsonii dbp-3.the denitrifying bacterium acinetobacter johnsonii strain dbp-3 which was capable of removing phosphate, nitrate, and ammoniacal salt is psychrotolerant, whereas, the cold shock response mechanisms or the cold shock proteins (csps) was unclear. in this article, the optimal growth temperature (25 °c) and cold shock temperature (7.5 °c) were determined firstly by an arrhenius plot of the growth of the strain dbp-3. then, among the seven cold shock-like protein genes which were cloned and identifie ...201626794214
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