| secondary structural and phylogenetic implications of nuclear large subunit ribosomal rna in the ectomycorrhizal fungus tricholoma matsutake. | the sequence of large subunit (lsu) and 5.8s rrna genes has been determined for tricholoma matsutake. a secondary structure model was predicted for both lsu and 5.8s rrnas, showing most of the structural features consistent with those of the consensus secondary structure model proposed for the eukaryotic cytoplasmic lsu rrnas. with a reconstructed eukaryotic phylogeny based on full-length lsu rdna sequences, t. matsutake was placed on the same branch with cryptococcus neoformans as its closest n ... | 2000 | 10688694 |
| identification of non-pseudomonad bacteria from fruit bodies of wild agaricales fungi that detoxify tolaasin produced by pseudomonas tolaasii. | bacterial isolates from wild agaricales fungi detoxified tolaasin, the inducer of brown blotch disease of cultivated mushrooms produced by pseudomonas tolaasii. mycetocola tolaasinivorans and mycetocola lacteus were associated with fruit bodies of wild pleurotus ostreatus and wild lepista nuda, respectively. tolaasin-detoxifying bacteria belonging to other genera were found in various wild mushrooms. an acinetobacter sp. was isolated from fruit bodies of tricholoma matsutake, bacillus pumilus wa ... | 2002 | 12450133 |
| [studies on the isolation, culture and dna identification of mycelia of tricholoma matsutake]. | the tissue isolation for tricholom matsutake(s ito et imai) sing were made with 8 media in 810 test tubes from different positions of 9 basidiocarps of different source and from mycorrhizae, and soil with the fungi in the studies. the results showed that 94 test tubes of slow-growing mycelia were isolated from lamellae and their success percentages of isolation with media pdas, pdaw, bm, pda were 74.4%, 355%, 15.6% and 8.9% respectively. the fast-growing mycelia were easily got from the mycorrhi ... | 2001 | 12549080 |
| purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete, tricholoma matsutake. | a new h(2)o(2)-generating pyranose oxidase was purified as a strong antifungal protein from an arbuscular mycorrhizal fungus, tricholoma matsutake. the protein showed a molecular mass of 250 kda in gel filtration, and probably consisted of four identical 62 kda subunits. the protein contained flavin moiety and it oxidized d-glucose at position c-2. h(2)o(2) and d-glucosone produced by the pyranose oxidase reaction showed antifungal activity, suggesting these compounds were the molecular basis of ... | 2003 | 14730138 |
| the mycorrhizal fungus tricholoma matsutake stimulates pinus densiflora seedling growth in vitro. | while it has been suggested that matsutake mycorrhizae might not be functional and that matsutake may behave as a saprobic fungus in soil or even have some pathogenic activity on seedlings, we investigated the consequences of matsutake inoculation on pinus densiflora growth. seventy-five days after inoculation, hyphae were anchored on short roots and well-developed hartig net palmettis were observed. compared to both control treatments--seedlings treated with distilled water and seedlings treate ... | 2004 | 15322965 |
| genetic mosaics in the massive persisting rhizosphere colony "shiro" of the ectomycorrhizal basidiomycete tricholoma matsutake. | the ectomycorrhizal basidiomycete tricholoma matsutake produces commercially valuable fruit bodies "matsutake" on a massive persisting rhizosphere aggregate of mycelia and mycorrhizas called "shiro." using inter-retrotransposon amplified polymorphism analysis, we attempted to explore the potential diversity within the population of t. matsutake isolated from small pinus densiflora woodlands located in various parts of japan. in general, random phylogenetic relationship was noted among t. matsuta ... | 2005 | 15830210 |
| ectomycorrhizal symbiosis in vitro between tricholoma matsutake and pinus densiflora seedlings that resembles naturally occurring 'shiro'. | we established an in vitro ectomycorrhizal symbiosis between tricholoma matsutake and pinus densiflora. mycorrhiza formed in a substrate of modified norkrans' c medium and granite-based soil had features similar to those observed previously only in naturally occurring mycorrhizal system called 'shiro,' and promoted the growth of plants with smaller root/shoot ratios. the in vitro formation of 'shiro' is essential for the development of t. matsutake system to produce mushrooms and is useful for t ... | 2006 | 16273392 |
| absorption and tissue distribution of an immunomodulatory alpha-d-glucan after oral administration of tricholoma matsutake. | alpha-d-glucan (mpg-1) separated from tricholoma matsutake (cm6271) has been reported to show immunomodulatory activities. in this study, the plasma concentration and tissue distribution of mpg-1 after cm6271 oral administration were investigated as part of the action mechanism analysis. when cm6271 was orally administered in a single dose to mice, mpg-1 was absorbed via the intestinal tract, appeared in plasma after 16 h, was gradually excreted from the blood, and fell to background level after ... | 2008 | 18680305 |
| isolation and characterization of a novel immunomodulatory alpha-glucan-protein complex from the mycelium of tricholoma matsutake in basidiomycetes. | tricholoma matsutake, a high-class edible mushroom in japan, has been reported to have excellent biological activities, but difficulty in cultivating the fruit bodies and limited bulk availability have restricted detailed studies. we have developed a method of culturing in tanks, enabling the bulk supply of the mycelia. the preparation (cm6271) exerts modulative effects on the immune competence of mice and rats. in this study, a sodium hydroxide extract of cm6271 was defatted followed by fractio ... | 2005 | 16277387 |
| successful inoculation of mature pine with tricholoma matsutake. | our finding demonstrates, for the first time, that the roots of mature pine trees can be successfully inoculated with a symbiotic ectomycorrhizal fungus, the valuable matsutake mushroom. long root segments (ca. 5-10 mm in diameter, ca. 50 cm in length) of 50-year-old pinus densiflora trees were excavated, washed, auxin-treated (2-5 mg indole butyric acid, iba, per root) and incubated in moist spagnum moss. twelve months later, short roots were regenerated, of which approximately 90% were free of ... | 2005 | 15856336 |
| highly polymorphic dna markers to specify strains of the ectomycorrhizal basidiomycete tricholoma matsutake based on sigmamary1, the long terminal repeat of gypsy-type retroelement mary1. | the ectomycorrhizal basidiomycete tricholoma matsutake produces commercially valuable fruit bodies--matsutake--in pinus sp. forest. here we report that pcr with outward facing primers designed based on sequences comprising sigma(mary1), the long terminal repeat of the gypsy-type retroelement mary1, specifies strains of t. matsutake. pcr with a primer based on the 22-bp sequence conserved at the 5'-end of sigma(mary1) conferred 73 reliable bands overall whose profiles depend upon strains of t. ma ... | 2005 | 15375684 |
| concentrations of 137cs and 40k in mushrooms consumed in japan and radiation dose as a result of their dietary intake. | mushrooms are known to accumulate radiocesium. to estimate the intake of radiocesium through the eating of mushrooms, about 30 samples belonging to 4 commonly consumed species (lentinula edodes, hypsizigus marmoreus, grifola frondosa, and tricholoma matsutake), were analyzed for (137)cs and (40)k. the concentration ranges were 0.060-29 bq kg(-1) (wet wt) for (137)cs and 38-300 bq kg(-1) (wet wt) for (40)k. the geometric mean concentration for (137)cs was 0.56 bq kg(-1) (wet wt), and the mean con ... | 2004 | 15304977 |
| [activation of antitumor immunity by intratumor injection of biological preparations]. | the antitumor effects of biological response modifiers (brms) in an experimental mouse model using a double grafted tumor system were analyzed. some brms prevented metastases by utilizing the anti-tumor immunological cascade reactions, which activate macrophages in the body. the following brms were analyzed: psk was a hot water extract of cultured mycelia from coliolus versicolor and a protein bound beta-glucan. lentinan was purified from fruit bodies of lentinus erodes and is a beta-glucan. the ... | 2003 | 14619462 |
| saprobic potential of tricholoma matsutake: growth over pine bark treated with surfactants. | saprotrophic growth of tricholoma matsutake isolates was investigated over pinus densiflora bark fragments either on soil or on agar media. preferential colonization of pine bark fragments by hyphae, in glucose-deprived environments suggested that matsutake was able to extract some nutrients to sustain its growth. this was confirmed in glucose-free liquid nutrient medium, where bark as sole carbon source significantly stimulated (up to two-fold) growth of t. matsutake isolates. the addition of s ... | 2002 | 11968941 |
| expression of mary1, a gypsy-type ltr-retroelement from the ectomycorrhizal homobasidiomycete tricholoma matsutake, in the budding yeast saccharomyces cerevisiae. | mary1 is a gypsy-type ltr-retroelement present in the genome of the ectomycorrhizal homobasidiomycete tricholoma matsutake. we document here that a mary1-lacz gene fusion was expressed in the budding yeast saccharomyces cerevisiae. the finding strongly suggests that mary1 is activated by trans-regulatory factors common to higher fungi, and may be useful for the development of new recombinant systems in ectomycorrhizal fungi and homobasidiomycetes. | 2001 | 11388490 |
| the nuclear rdna intergenic spacer of the ectomycorrhizal basidiomycete laccaria bicolor: structural analysis and allelic polymorphism. | the nuclear rdna intergenic spacer (igs) of the ectomycorrhizal basidiomycete laccaria bicolor was amplified and sequenced to identify the source of its intraspecific polymorphism. the igs was amplified by pcr in several l. bicolor strains and shown to exhibit multiple bands and length polymorphism. the igs loci were shown to segregate in a 1:1 ratio within haploid progenies in three dikaryotic strains, suggesting that divergent igs haplotypes were present in the two nuclei of these strains. the ... | 1999 | 10439399 |
| [quantities of agaritine in mushrooms (agaricus bisporus) and the carcinogenicity of mushroom methanol extracts on the mouse bladder epithelium]. | consumption of the agaricus species mushrooms has increased considerably in japan as the japanese have become accustomed to western cooking. the agaricus species mushroom contains hydrazine derivatives known as agaritine. bladder implantation was performed to test the carcinogenic potential of the agaricus species mushroom which contains large quantities of agaritine. the results are summarized as follows: 1) agaritine was detected in fresh agaricus bisporus mushrooms at the level of 228.2 micro ... | 1990 | 2132000 |
| mushrooms, trees, and money: value estimates of commercial mushrooms and timber in the pacific northwest. | wild edible mushrooms are harvested in the forests of the pacific northwest, where both trees and mushrooms grow in the same landscape. although there has been some discussion about the value of trees and mushrooms individually, little information exists about the joint production of, and value for, these two forest products. through four case studies, the information needed to determine production and value for three wild mushroom species in different forests of the pacific northwest is describ ... | 2002 | 12053246 |
| phylogenetic relationship and species delimitation of matsutake and allied species based on multilocus phylogeny and haplotype analyses. | tricholoma matsutake (s. ito & s. imai) singer and its allied species are referred to as matsutake worldwide and are the most economically important edible mushrooms in japan. they are widely distributed in the northern hemisphere and established an ectomycorrhizal relationship with conifer and broadleaf trees. to clarify relationships among t. matsutake and its allies, and to delimit phylogenetic species, we analyzed multilocus datasets (its, megb1, tef, gpd) with samples that were correctly id ... | 2012 | 22684294 |
| ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from tricholoma matsutake. | an ultrasonic-assisted technique was employed to extract crude polysaccharide from tricholoma matsutake fruiting bodies. single-factor tests and orthogonal experimental design (l9(3(3))) were used to obtain the optimal extraction conditions. results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °c; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 khz; and ultrasonic power, 100 w. three novel t. matsutake polysaccharide (tmp) ... | 2017 | 28786242 |
| tricholoma matsutake can absorb and accumulate trace elements directly from rock fragments in the shiro. | tricholoma matsutake, a highly valued delicacy in japan and east asia, is an ectomycorrhizal fungus typically found in a complex soil community of mycorrhizae, soil microbes, and host-tree roots referred to as the shiro in japan. a curious characteristic of the shiro is an assortment of small rock fragments that have been implicated as a direct source of minerals and trace elements for the fungus. in this study, we measured the mineral content of 14 samples of shiro soil containing live matsutak ... | 2015 | 25355073 |
| bak is a key molecule in apoptosis induced by methanol extracts of codonopsis lanceolata and tricholoma matsutake in hsc-2 human oral cancer cells. | since the 5-year survival rate of oral cancer remains low, more effective and non-toxic therapeutic and preventive strategies are required. certain natural products possess anti-cancer properties. the present study investigated the effects of the methanol extracts of codonopsis lanceolata (meci) and tricholoma matsutake (metm) and identified the molecular target in hsc-2 human oral cancer cells. the results revealed that meci and metm inhibited growth and induced apoptosis, as demonstrated by po ... | 2012 | 23205139 |
| north american matsutake: names clarified and a new species described. | tricholoma matsutake, known widely as "matsutake," has great commercial and cultural significance in japan. because japanese production is insufficient to meet the high domestic demand, morphologically similar mushrooms, thought by many to belong to t. magnivelare, are imported from western north america. however, molecular data produced since the early 2000s have indicated that more than one species of matsutake occur in north america and this raises the question of correct naming for the diffe ... | 2017 | 28609221 |
| strategies of functional food for cancer prevention in human beings. | functional food for prevention of chronic diseases is one of this century's key global challenges. cancer is not only the first or second leading cause of death in china and other countries across the world, but also has diet as one of the most important modifiable risk factors. major dietary factors now known to promote cancer development are polished grain foods and low intake of fresh vegetables, with general importance for an unhealthy lifestyle and obesity. the strategies of cancer preventi ... | 2013 | 23679240 |
| trichoderma songyi sp. nov., a new species associated with the pine mushroom (tricholoma matsutake). | a new species, trichoderma songyi, was found to be associated with the pine mushroom (tricholoma matsutake) in korea. this species was isolated from three different substrates: tricholoma matsutake basidiomata, as well as roots of pinus densiflora and soil in the fairy ring. based on its molecular and phenotypic characteristics, we demonstrate that trichoderma songyi is unique and distinguishable from closely related species. we performed phylogenetic analyses based on two molecular markers, the ... | 2014 | 25052534 |
| anti-microorganism, anti-tumor, and immune activities of a novel polysaccharide isolated from tricholoma matsutake. | many more fungal polysaccharides have been reported to exhibit a variety of biological activities, including anti-tumor, immunostimulation, anti-oxidation, and so on. the non-starch polysaccharides have emerged as an important class of bioactive natural products. | 2013 | 23930009 |
| distinctive feature of microbial communities and bacterial functional profiles in tricholoma matsutake dominant soil. | tricholoma matsutake, the pine mushroom, is a valuable forest product with high economic value in asia, and plays an important ecological role as an ectomycorrhizal fungus. around the host tree, t. matsutake hyphae generate a distinctive soil aggregating environment called a fairy ring, where fruiting bodies form. because t. matsutake hyphae dominate the soil near the fairy ring, this species has the potential to influence the microbial community. to explore the influence of t. matsutake on the ... | 2016 | 27977803 |
| tricholoma matsutake dominates diverse microbial communities in different forest soils. | fungal and actinobacterial communities were analyzed together with soil chemistry and enzyme activities in order to profile the microbial diversity associated with the economically important mushroom tricholoma matsutake. samples of mycelium-soil aggregation (shiro) were collected from three experimental sites where sporocarps naturally formed. pcr was used to confirm the presence and absence of matsutake in soil samples. pcr-denaturing gradient gel electrophoresis (dgge) fingerprinting and dire ... | 2011 | 21984247 |
| ectomycorrhization of tricholoma matsutake and two major conifers in finland-an assessment of in vitro mycorrhiza formation. | this study aimed to test the ability of tricholoma matsutake isolates to form mycorrhizas with aseptic seedlings of pinus sylvestris l. and picea abies (l.) karst. germinated seedlings of scots pine and norway spruce were separately inoculated with either isolates originating from finland or japan. eight months after inoculation, the finnish isolate had formed a sheath and hartig net on both host species. ectomycorrhizal scots pine seedlings inoculated with the finnish isolate showed the same sh ... | 2010 | 20177716 |
| in vitro ectomycorrhizal specificity between the asian red pine pinus densiflora and tricholoma matsutake and allied species from worldwide pinaceae and fagaceae forests. | tricholoma matsutake produces commercially valuable, yet uncultivable, mushrooms (matsutake) in association with pines in the far east and scandinavia and with both pines and oaks in the foothills of tibet. other matsutake mushrooms, such as tricholoma anatolicum from the mediterranean regions and tricholoma magnivelare and tricholoma sp. from the north pacific coast area of canada and north america as well as mexico, respectively, are associated with pines or oaks in their natural habitats. tri ... | 2010 | 19941149 |
| association analysis of food allergens. | food allergy patients are known to present with allergic reactions to multiple allergens, but extrapolating these associations is difficult. data mining, a procedure that analyzes characteristic combinations among large amounts of information, is often used to analyze and predict consumer purchasing behaviour. we applied this technique to the extrapolation of food allergen associations in allergy patients. we sent 1510 families our 'questionnaire survey for the prevention of food allergies'. res ... | 2009 | 19538355 |
| development of an enzyme-linked immunosorbent assay to detect an immunomodulatory alpha-d-glucan-protein complex, mpg-1, in basidiomycete tricholoma matsutake and related processed foods. | we previously isolated a novel immunomodulatory alpha-(1,4)(1,6)(1,2)- d-glucan-protein complex (mpg-1) from mycelia of tricholoma matsutake in basidiomycetes. in the present study, we raised a polyclonal antibody by immunizing rabbits with mpg-1 and constructed a sandwich enzyme-linked immunosorbent assay (elisa) system to examine the distribution of mpg-1 among edible mushrooms and related processed foods. the system detected mpg-1 quantitatively at concentrations of more than 10 ng/ml, with a ... | 2007 | 17867639 |
| tricholoma matsutake in a natural pinus densiflora forest: correspondence between above- and below-ground genets, association with multiple host trees and alteration of existing ectomycorrhizal communities. | tricholoma matsutake (matsutake) is an ectomycorrhizal (ecm) fungus that produces economically important mushrooms in japan. here, we use microsatellite markers to identify genets of matsutake sporocarps and below-ground ecm tips, as well as associated host genotypes of pinus densiflora. we also studied ecm fungal community structure inside, beneath and outside the matsutake fairy rings, using morphological and internal transcribed spacer (its) polymorphism analysis. based on sporocarp samples, ... | 2006 | 16918553 |
| [antitumor effects of intratumoral injection of basidiomycetes preparations]. | the antitumor effects of basidiomycetes preparations in an experimental mouse model, the "double grafted tumor system" were analyzed. some brms prevented metastases by utilizing the anti-tumor immunological cascade reactions, which activates macrophages in the body. the following basidiomycetes preparations were analyzed: psk was a hot water extract of cultured mycelia from coliolus versicolor and a protein bound beta-glucan. matsumax was extracted from mycelia of tricholoma matsutake and was a ... | 2005 | 16315899 |
| cloning and expression analysis of phenylalanine ammonia-lyase gene in the mycelium and fruit body of the edible mushroom flammulina velutipes. | phenylalanine ammonia-lyase (pal) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. however, its expression in fungi remains to be explored. to understand its expression in mushroom fungi, the pal gene of the edible mushroom flammulina velutipes (fvpal) was cloned and characterized. the cloned fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. the translated amino aci ... | 2015 | 26539050 |
| terpenoids and sterols from some japanese mushrooms. | over the past twenty years, our research group has been studying the chemical constituents of mushrooms. from nineteen species, namely, amanita virgineoides bas (amanitaceae), daedaleopsis tricolor (bull.: fr.) bond. et sing. (polyporaceae), grifolafrondosa (fr.) s. f. gray (polyporaceae), hericium erinaceum (bull.: fr.) pers. (hericiaceae), hypsizigus marmoreus (peck) bigelow (tricholomataceae), lactarius piperatus (scop.: fr.) s. f. gray (russulaceae), lentinula edodes (berk.) sing. (pleurotac ... | 2014 | 24689228 |
| new sterols and triterpenoids from four edible mushrooms. | four edible mushrooms, panellus serotinus, lepista nuda, tricholoma matsutake and naematoloma sublateritium, have been investigated chemically. two new sterols, 5alpha,9alpha-epidioxy-(22e)-ergosta-7,22-diene-3beta,6alpha-diol (1) and 5alpha,9alpha-epidioxy-(22e)-ergosta-7,22-diene-3beta,6beta-diol (2), have been isolated from panellus serotinus. compound 2 was also isolated from lepista nuda. a new sterol, 3beta,5alpha,9alpha,14beta-tetrahydroxy-(22e)-ergosta-7,22-dien-6-one (3), and compound 2 ... | 2001 | 11383612 |
| cloning and mrna expression analysis of the gene encoding phenylalanine ammonia-lyase of the ectomycorrhizal fungus tricholoma matsutake. | the ectomycorrhizal fungus tricholoma matsutake grows symbiotically with pinus densiflora. phenylalanine ammonia-lyase (e.c. 4.3.1.24) catalyzes the conversion of l-phenylalanine to trans-cinnamic acid. the role of fungal phenylalanine ammonia-lyase, however, has not been clear until now. in this study, the gene encoding phenylalanine ammonia-lyase (pal), which was isolated from t. matsutake, was cloned and characterized. the pal gene (tmpal) consists of 2,160 nucleotides, coding for a polypepti ... | 2013 | 23801251 |
| extract of the mycelium of t. matsutake inhibits elastase activity and tpa-induced mmp-1 expression in human fibroblasts. | skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ecm). collagen and elastin degradation can induce the expression of matrix metalloproteinases (mmps), as well as that of a family of zinc-dependent endopeptidases that play critical roles in skin aging. moreover, elastase is a metalloproteinase which acts on the degradation of elastin in skin aging, and is also involve ... | 2014 | 25319362 |
| mineral element levels in wild edible mushrooms from yunnan, china. | ten species of wild edible mushrooms (boletus griseus, boletus speciosus, lactarius hygrophoroides, leucopaxillus giganteus, macrocybe gigantea, melanoleuca arcuata, morchella deliciosa, mycena haematopus, pulveroboletus ravenelii, and tricholoma matsutake) collected from yunnan province of china, were analyzed for ten mineral elements (calcium, chromium, cobalt, copper, iron, magnesium, manganese, potassium, sodium, and zinc) contents using icp-aes. the minimum and maximum element contents of m ... | 2012 | 22234825 |
| the host ranges of conifer-associated tricholoma matsutake, fagaceae-associated t. bakamatsutake and t. fulvocastaneum are wider in vitro than in nature. | tricholoma matsutake is the most commercially important edible mushroom in pine forests in japan. tricholoma bakamatsutake and t. fulvocastaneum, species closely related to t. matsutake, occur in fagaceae forests. we examined ectomycorrhizal (em) formation by these tricholoma species by in vitro synthesis among seven strains (two of t. matsutake, four of t. bakamatsutake, one of t. fulvocastaneum) and axenic plants of pine (pinus densiflora) and oak (quercus serrata, q. phillyraeoides). all stra ... | 2014 | 24871598 |
| structure elucidation and antitumor activity of a new polysaccharide from maerkang tricholoma matsutake. | a new heteropolysaccharide was isolated from the fruiting bodies of tricholoma matsutake which had a molecular weight of 12078 da. the results of structural features analysis showed that t. matsutake polysaccharide, here named tmp-b, was mainly composed of α - d - glucose and α - d - galactose which ratios were 7:2 and had a backbone of 1, 4 - linked α - d - glucose which branches were mainly composed of two 6 - linked α - d - galactose residue, and the α - d - galactose was 1, 6 - linked. antit ... | 2017 | 28808425 |
| polysaccharides from tricholoma matsutake and lentinus edodes enhance 5-fluorouracil-mediated h22 cell growth inhibition. | few studies have investigated the effects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment. we hypothesized that a combination of polysaccharides (cop) from lentinus edodes and tricholoma matsutake would improve the efficacy of 5-fluorouracil (5-fu)-mediated inhibition of h22 cell growth. | 2014 | 24992758 |
| investigation of the immunomodulatory activity of tricholoma matsutake mycelium in cyclophosphamide-induced immunosuppressed mice. | tricholoma matsutake, a popular food and biopharmaceutical resource in asia, possesses various pharmacological activities. although t. matsutake mycelium (tm) may enhance immunity, previous studies, to the best of our knowledge, have been performed on normal animals or cells alone. the present study aimed to evaluate the immunomodulatory activity of tm at doses of 0.3, 1.0 and 2.0 g/kg in cyclophosphamide (ctx)‑induced immunosuppressed mouse models. tm treatment for 2 weeks markedly improved the ... | 2017 | 28765877 |
| mobile dna distributions refine the phylogeny of "matsutake" mushrooms, tricholoma sect. caligata. | "matsutake" mushrooms are formed by several species of tricholoma sect. caligata distributed across the northern hemisphere. a phylogenetic analysis of matsutake based on virtually neutral mutations in dna sequences resolved robust relationships among tricholoma anatolicum, tricholoma bakamatsutake, tricholoma magnivelare, tricholoma matsutake, and tricholoma sp. from mexico (=tricholoma sp. mex). however, relationships among these matsutake and other species, such as tricholoma caligatum and tr ... | 2013 | 23440576 |
| a comparison study on extraction of polysaccharides from tricholoma matsutake by response surface methodology. | the tricholoma matsutake polysaccharides (tmp) extraction was optimized using counter-current probe ultrasonic extraction (ccpue) by response surface methodology (rsm). the effect of three factors (liquid-solid ratio, extraction time and pulse duration) on the tmp yield was studied. the results showed that the optimal conditions were liquid-solid ratio (15), extraction time (61 min) and pulse duration (6.0 s). under best conditions, the maximum tmp yield was 8.11 ± 0.35%, which was close with th ... | 2014 | 24507300 |
| matsutakone and matsutoic acid, two (nor)steroids with unusual skeletons from the edible mushroom tricholoma matsutake. | matsutakone (1), a novel sterol with an unprecedented polycyclic ring system, together with a new norsteroid matsutoic acid (2) were isolated from the fruiting bodies of tricholoma matsutake. their structures and absolute configurations were assigned by extensive spectroscopic analyses and computational methods. bioassay results showed that compounds 1 and 2 exhibited inhibitory activities against acetylcholinesterase (ic50 20.9 μm for 1). | 2017 | 28691489 |
| effects of matsutake mushroom scent compounds on tyrosinase and murine b16-f10 melanoma cells. | tyrosinase-catalyzed l-tyrosine oxidation is a key step in melanogenesis, and intense melanin formation is often a problem in chemotherapies or food preservation. here we report that methyl cinnamate one of the constituents characterized from mycelium and sporocarp of american matsutake mushroom tricholoma magnivelare inhibits both enzymatic and cellular melanin formation. methyl cinnamate inhibits mushroom tyrosinase-catalyzed l-tyrosine oxidation while the oxidation of l-3,4-dihydroxyphenylala ... | 2017 | 28456625 |
| prediction of the potential geographic distribution of the ectomycorrhizal mushroom tricholoma matsutake under multiple climate change scenarios. | effective conservation and utilization strategies for natural biological resources require a clear understanding of the geographic distribution of the target species. tricholoma matsutake is an ectomycorrhizal (ecm) mushroom with high ecological and economic value. in this study, the potential geographic distribution of t. matsutake under current conditions in china was simulated using maxent software based on species presence data and 24 environmental variables. the future distributions of t. m ... | 2017 | 28393865 |
| tricholoma matsutake aqueous extract induces hepatocellular carcinoma cell apoptosis via caspase-dependent mitochondrial pathway. | tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. our study aims to evaluate the cytotoxicity activities of t. matsutake aqueous extract (tm) in hepg2 and smmc-7721 cells. in in vitro experiments, tm strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ros, and caused caspases cascade and mitochond ... | 2016 | 28018916 |
| the (oxalato)aluminate complex as an antimicrobial substance protecting the "shiro" of tricholoma matsutake from soil micro-organisms. | tricholoma matsutake, a basidiomycete, forms ectomycorrhizas with pinus densiflora as the host tree. its fruiting body, "matsutake" in japanese, is an edible and highly prized mushroom, and it grows in a circle called a fairy ring. beneath the fairy ring of t. matsutake, a whitish mycelium-soil aggregated zone, called "shiro" in japanese, develops. the front of the shiro, an active mycorrhizal zone, functions to gather nutrients from the soil and roots to nourish the fairy ring. bacteria and spo ... | 2017 | 27691719 |
| a qpcr assay that specifically quantifies tricholoma matsutake biomass in natural soil. | tricholoma matsutake is an ectomycorrhizal basidiomycete that produces prized, yet uncultivable, "matsutake" mushrooms along densely developed mycelia, called "shiro," in the rhizosphere of coniferous forests. pinus densiflora is a major host of this fungus in japan. measuring t. matsutake biomass in soil allows us to determine the kinetics of fungal growth before and after fruiting, which is useful for analyzing the conditions of the shiro and its surrounding mycorrhizosphere, predicting fruiti ... | 2016 | 27371100 |
| comparative analysis of macrophage transcriptomes reveals a key mechanism of the immunomodulatory activity of tricholoma matsutake polysaccharide. | in the present study, we performed a proliferation assay, phagocytosis assay and cell cycle analysis of macrophages and sequenced the transcriptomes of control group macrophages and tmp-a group macrophages using illumina sequencing technology to identify differentially expressed genes (degs) and determine the molecular mechanisms associated with differences in the immunomodulatory activity of tmp-a in macrophages. the results showed that tmp-a exhibits strong proliferation activity and phagocyto ... | 2016 | 27221808 |
| a tricholoma matsutake peptide with angiotensin converting enzyme inhibitory and antioxidative activities and antihypertensive effects in spontaneously hypertensive rats. | hypertension is a major risk factor for cardiovascular disease. a crude water extract of the fruiting bodies of a highly prized mushroom tricholoma matsutakei exerted an antihypertensive action on spontaneously hypertensive rats (shrs) at a dosage of 400 mg/kg. an angiotensin converting enzyme (ace) inhibitory peptide with an ic50 of 0.40 μm was purified from the extract and designated as tmp. its amino acid sequence was elucidated to be walkgyk through lc-ms/ms analysis. the lineweaver-burk plo ... | 2016 | 27052674 |
| antifatigue activity of liquid cultured tricholoma matsutake mycelium partially via regulation of antioxidant pathway in mouse. | tricholoma matsutake has been popular as food and biopharmaceutical materials in asian countries for its various pharmacological activities. the present study aims to analyze the antifatigue effects on enhancing exercise performance of tricholoma matsutake fruit body (abm) and liquid cultured mycelia (tm) in mouse model. two-week tricholoma matsutake treatment significantly enhances the exercise performance in weight-loaded swimming, rotating rod, and forced running test. in tm- and abm-treated ... | 2015 | 26697489 |
| analysis of bacterial diversity and communities associated with tricholoma matsutake fruiting bodies by barcoded pyrosequencing in sichuan province, southwest china. | endophytes play an important role in the growth and development of the host. however, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. we studied the bacteria associated with fruiting bodies of tricholoma matsutake picked from seven main t. matsutake-producing areas in sichuan, china, by barcoded pyrosequencing. about 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and arch ... | 2016 | 26428733 |
| a fungal α-galactosidase from tricholoma matsutake with broad substrate specificity and good hydrolytic activity on raffinose family oligosaccharides. | an acidic α-galactosidase designated as tmg was purified from the fruiting bodies the purification protocol entailed ion exchange chromatography on q-sepharose and of tricholoma matsutake with 136-fold purification and a specific activity of 909 units/mg. mono-q and fast protein liquid chromatography on superdex 75. tmg is a monomeric protein exhibiting a molecular mass of 47 kda in sds-page and gel filtration. the purified enzyme was identified by lc-ms/ms and three inner amino acid sequences w ... | 2015 | 26213909 |
| isolation and structural elucidation of a novel homogenous polysaccharide from tricholoma matsutake. | a crude polysaccharide possessing antitumour, radiation-resistant and anti-ageing attributes was extracted from tricholoma matsutake by water extraction and alcohol precipitation. from this crude polysaccharide, a homogeneous polysaccharide, tmp-5ii, was successfully purified by sephacryl s-300 column chromatography. the average molecular weight (mw) of tmp-5ii was 15.76 kda. monosaccharide analysis indicated that the homogeneous polysaccharide contained four different residues: d-glucose, d-gal ... | 2016 | 26208132 |
| [community structure and diversity of entophytic bacteria in tricholoma matsutake in sichuan province, southwest china]. | in order to understand the diversity and community structure of endophytic bacteria in tricholoma matsutake, 14 tricholoma matsutake samples were collected from 7 main production counties of xiaojin, yajiang, muli, yanyuan, yanbian, huidong and mianning. the entophytic bacterial community structure and diversity were investigated by pcr denaturing gradient gel electrophoresis (pcr-dgge). the results showed that the diversity and community structure of endophytic bacteria in t. matsutake varied s ... | 2014 | 25898631 |
| tricholoma matsutake fruit bodies secrete hydrogen peroxide as a potent inhibitor of fungal growth. | tricholoma matsutake is an ectomycorrhizal fungus that dominates the microbial communities in the soil of pine and spruce forests. the mycorrhizas of this fungus have antimicrobial activity, although factors responsible for the antimicrobial activity have not been fully elucidated. the present study shows that fruit bodies of t. matsutake secreted hydrogen peroxide (h2o2), which was produced by pyranose oxidase, and that the h2o2 thus secreted strongly inhibited the growth of mycelia of the phyt ... | 2015 | 25803209 |
| a novel laccase from fresh fruiting bodies of the wild medicinal mushroom tricholoma matsutake. | the knowledge about biological activities of constituents from medicinal mushrooms belonging to the genus tricholoma is limited. a 59-kda laccase has now been purified from fresh fruiting bodies of the mushroom tricholoma matsutake. the purification protocol entailed ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on cm-cellulose, affinity chromatography on cona-sepharose, and gel filtration by fast protein liquid chromatog ... | 2015 | 25781157 |
| purification and properties of a nuclease from the fruit body of tricholoma matsutake. | a nuclease was purified from the fruit body of tricholoma matsutake. the molecular mass was 38 kda. the optimum ph of the nuclease was about 8.0. its activity was inhibited by gtp. the nuclease cleaved rna and heat-denatured dna endonucleolytically to produce 5'-mononucleotide, and showed 3'-nucleotidase activity. the amino acid sequence up to seven amino acids from the n-terminus was nh(2)-apppssn. | 2010 | 20057129 |
| genetic variability and bottleneck detection of four tricholoma matsutake populations from northeastern and southwestern china. | the excessive commercial collection of matsutake mushrooms can lead to extreme reduction of population size, which may cause genetic bottleneck and decrease genetic diversity of tricholoma matsutake. here, six polymorphic microsatellite loci markers were used to examine the genetic diversity of four natural t. matsutake populations from two main producing regions of china. the minimum combinations of four loci were able to discriminate total 86 sampled individuals with distinctive multilocus gen ... | 2015 | 25682708 |
| root endophyte symbiosis in vitro between the ectomycorrhizal basidiomycete tricholoma matsutake and the arbuscular mycorrhizal plant prunus speciosa. | we previously reported that tricholoma matsutake and tricholoma fulvocastaneum, ectomycorrhizal basidiomycetes that associate with pinaceae and fagaceae, respectively, in the northern hemisphere, could interact in vitro as a root endophyte of somatic plants of cedrela odorata (meliaceae), which naturally harbors arbuscular mycorrhizal fungi in south america, to form a characteristic rhizospheric colony or "shiro". we questioned whether this phenomenon could have occurred because of plant-microbe ... | 2014 | 24158697 |
| ergosterol and water changes in tricholoma matsutake soil colony during the mushroom fruiting season. | the purpose of this study is to understand spatio-temporal changes of active fungal biomass and water in tricholoma matsutake soil colonies during the mushroom fruiting season. the active fungal biomass was estimated by analyzing ergosterol content at four different points within four replicated locations in a single circular t. matsutake colony at ssanggok valley in the sogri mt. national park in korea during 2003 to 2005. the four points were the ahead of the colony, the front edge of the colo ... | 2009 | 23983500 |
| root endophyte interaction between ectomycorrhizal basidiomycete tricholoma matsutake and arbuscular mycorrhizal tree cedrela odorata, allowing in vitro synthesis of rhizospheric "shiro". | the ectomycorrhizal basidiomycete tricholoma matsutake associates with members of the pinaceae such as pinus densiflora (red pine), forming a rhizospheric colony or "shiro," which produces the prized "matsutake" mushroom. we investigated whether the host specificity of t. matsutake to conifers is innately determined using somatic plants of cedrela odorata, a tropical broad-leaved tree (meliaceae) that naturally harbors arbuscular mycorrhizal fungi. we found that t. matsutake could form in vitro ... | 2013 | 23064771 |
| development of microsatellite markers from an ectomycorrhizal fungus, tricholoma matsutake, by an issr-suppression-pcr method. | an inter-simple sequence repeat (issr)-suppression-pcr technique established to develop microsatellite markers of plant species was applied to an ectomycorrhizal fungus, tricholoma matsutake. six polymorphic ssr markers were developed. all six polymorphic ssr markers were single-locused and co-dominant. alleles produced by these six single-locused markers ranged from two to nine per locus and the expected heterozygosities were calculated as values from 0.098 to 0.803. the results indicated that ... | 2003 | 12634916 |
| extraction and purification of polysaccharides from pine medicinal mushroom, tricholoma matsutake (higher basidiomycetes) fruit bodies. | ultrasound-assisted extraction, purification, and characterization of antioxidant polysaccharides from tricholoma matsutake were carried out. on the basis of the results of a single-factor test, three independent and main variables, including extraction time (x1: 130-170 s), ultrasonic power (x2: 340-380 w), and ratio of water to raw material (x3: 45-55 ml/g) were studied by box-behnken design. the optimum extraction conditions are as follows: extraction time 160 s, ultrasonic power 365 w, and r ... | 2014 | 24941036 |
| comparative analysis of bacterial diversity and communities inhabiting the fairy ring of tricholoma matsutake by barcoded pyrosequencing. | comparative analysis of the soil bacterial communities inhabiting the fairy ring of tricholoma matsutake. | 2014 | 24922184 |
| detection of the edible ectomycorrhizal fungus lyophyllum shimeji colonising seedlings of cultivated conifer species in new zealand. | lyophyllum shimeji is an edible ectomycorrhizal fungus that is widely distributed in east asia and also present in the northern regions of europe. in japan, l. shimeji is a culinary delicacy, considered amongst all edible mushrooms to have the best taste and to be second only to tricholoma matsutake in price. traditionally, fruiting bodies of l. shimeji have been collected from the wild but fruiting of l. shimeji is now relatively uncommon and cannot keep up with increasing consumer demand. as a ... | 2014 | 24445577 |
| identification and analysis of the constituents in an aqueous extract of tricholoma matsutake by hplc coupled with diode array detection/electrospray ionization mass spectrometry. | the main constituents in an aqueous extract of tricholoma matsutake (tm) were identified by high-performance liquid chromatography coupled with diode array detection and electrospray ionization time-of-flight mass spectrometry (hplc-dad/tof-ms) and ion trap mass spectrometry (hplc-dad/trap-msn). the main factors in the extraction process which affect the yields of nutrients were optimized by single-factor experiments and orthogonal experiment design. in total, 12 constituents were identified fro ... | 2013 | 23957403 |
| metagenomic analysis of fungal communities inhabiting the fairy ring zone of tricholoma matsutake. | tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of pinus denisflora, forms a fruiting body that serves as a valuable food in asia. however, the artificial culture of this fungus has not been successful. soil fungi, including t. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of t. matsutake. here, we report on the structures of fungal communities associat ... | 2013 | 23949332 |
| calcium sensitizers isolated from the edible pine mushroom, tricholoma matsutake (s. ito & imai) sing. | three lactam compounds were isolated from the fruiting body of tricholoma matsutake (s. ito & imai) sing., an edible mushroom, and their structures were identified as cyclo-s-proline-r-leucine (1), hexahydro-2h-azepin-2-one (2), and butyl 5-oxo-2-pyrrolidine carboxylate (3) by chemical, physicochemical, and spectral evidence. in in vitro screening tests, compounds 1 and 2 acted as calcium sensitizers in ventricular cells from rat. further studies on compounds 1 and 2 in ex vivo isolated right at ... | 2013 | 23819306 |
| structural characterisation of polysaccharides from tricholoma matsutake and their antioxidant and antitumour activities. | in this study, polysaccharides from tricholoma matsutake (tm-p) were purified using a deae-sepharose fast flow column and three polysaccharide fractions (tm-p1, tm-p2 and tm-p3) were obtained. the chemical composition and structural characteristics of tm-p2 were quite different from those of tm-p1 and tm-p3. tm-p2 consisted of glucose, galactose and mannose with a molar ratio of 5.9:1.1:1.0. the glycosidic linkages were mainly composed of 1,6- and 1-linked glucose. furthermore, tm-p2 showed the ... | 2013 | 23497882 |
| purification, chemical characterization and radical scavenging activities of alkali-extracted polysaccharide fractions isolated from the fruit bodies of tricholoma matsutake. | tricholoma matsutake, a high-class edible mushroom in china, has been regarded as famous foods and biopharmaceutical materials with a great deal of interest. in the previous investigations, researchers believe the water-soluble polysaccharide β-glucan is the major active component of t. matsutake, which displays various biological activities. in the present study, two novel alkali-extracted polysaccharide fractions, tm-aps-1 and tm-aps-2, were isolated from the fruit bodies of t. matsutake by de ... | 2013 | 23242547 |
| [applicability of gc, gc/ms and liquid chromatography with tandem mass spectrometry to screening for 140 pesticides in agricultural products]. | the applicability of gc, gc/ms and liquid chromatography with tandem mass spectrometry (lc/ms/ms) to screening for 140 pesticides in agricultural products was examined. validation of multi-residue screening methods for the determination of 88 pesticides in 12 crops (asparagus, cauliflower, burdock, carrot, broccoli, spinach, matsutake mushroom, orange, soybean, sesame, millet and tea) was done by gc and gc/ms. of the 88 pesticides, 63 were obtained with recoveries in the range from 50 to 150% at ... | 2006 | 17128873 |
| a pediatric case of anaphylaxis caused by matsutake mushroom (tricholoma matsutake) ingestion. | anaphylaxis is one of the severest forms of allergic diseases. some kinds of mushroom are known as causative allergens in food anaphylaxis. matsutake mushroom (tricholoma matsutake) is a typical edible mushroom available in autumn in japan. we encountered an 8-year-old japanese girl who developed anaphylaxis after ingesting matsutake mushrooms. | 2006 | 17075291 |
| detecting nonculturable bacteria in the active mycorrhizal zone of the pine mushroom tricholoma matsutake. | the fungus tricholoma matsutake forms an ectomycorrhizal relationship with pine trees. its sporocarps often develop in a circle, which is commonly known as a fairy ring. the fungus produces a solid, compact, white aggregate of mycelia and mycorrhizae beneath the fairy ring, which in japanese is called a 'shiro'. in the present study, we used soil dilution plating and molecular techniques to analyze the bacterial communities within, beneath, and outside the t. matsutake fairy ring. soil dilution ... | 2012 | 22538647 |
| immunomodulatory activities of different solvent extracts from tricholoma matsutake (s. ito et s. imai) singer (higher basidiomycetes) on normal mice. | the immunomodulatory activities of different solvent extracts from the culinary-medicinal mushroom tricholoma matsutake were studied in vivo in normal mice. the extracts were prepared using different solvents in an order of increasing polarity. the immunomodulatory activities were investigated by measuring the thymus and spleen index, phagocytic rate of macrophage phagocytosis, delayed-type hypersensitivity, plaque-forming cell, and proliferation of splenocytes. results demonstrated that water e ... | 2012 | 23510248 |
| the ectomycorrhizal fungus tricholoma matsutake is a facultative saprotroph in vitro. | tricholoma matsutake is an economically important ectomycorrhizal fungus of coniferous woodlands. mycologists suspect that this fungus is also capable of saprotrophic feeding. in order to evaluate this hypothesis, enzyme and chemical assays were performed in the field and laboratory. from a natural population of t. matsutake in southern finland, samples of soil-mycelium aggregate (shiro) were taken from sites of sporocarp formation and nearby control (pcr-negative) spots. soil organic carbon and ... | 2012 | 22005782 |
| [optimization of ultrasonic-assisted extraction conditions of polysaccharides from tricholoma matsutake in meng mountain by response surface methodology]. | to obtain the optimal conditions of ultrasonic-assisted extraction of polysaccharides from fruitbody of tricholoma matsutake in meng mountain, and to provide the theoretical foundation for the further processing and utilization of tricholoma matsutake in meng mountain. | 2010 | 21137374 |
| a case of anaphylactic reaction following matsutake mushroom ingestion: demonstration of histamine release reaction of basophils. | matsutake mushroom is not recognized as a common food allergen. however, several case reports have suggested that this mushroom can induce anaphylaxis on rare occasions. | 2010 | 20864796 |
| spore dispersion of tricholoma matsutake at a pinus densiflora stand in korea. | the spore of tricholoma matsutake is considered to be the starting point of the mushroom growth cycle, but the mechanism of mycelial development from the spore stage is not yet clarified. in this study, we tried to measure how far the spores of t. matsutake disperse from a fruiting body located at a pinus densiflora stand in korea. we established 16 slide glasses coated with glycerin near a fruiting body in four directions separated by four different distance intervals within a mushroom producti ... | 2010 | 23956655 |
| growth promotion of mycelia of the matsutake mushroom tricholoma matsutake by d-isoleucine. | mycelial growth of the matsutake mushroom (tricholama matsutake) was much slower than that of the other mushroom species. we found that the addition of d-isoleucine to the culture medium strikingly promoted mycelia growth. the other amino acids tested had no effect on this growth promotion. | 2004 | 15564685 |
| structure elucidation and antioxidant activity of a novel polysaccharide isolated from tricholoma matsutake. | in this study, structural features of tricholoma matsutake polysaccharide (tmp-a) were investigated by a combination of infrared (ir) spectra, gas chromatography-mass spectrometry (gc-ms), nuclear magnetic resonance (nmr) spectroscopy. the results indicated that tmp-a had a backbone of 1,4-beta-d-glucopyranose residue which branches at o-6 based on the experimental results. the branches were mainly composed of an (1-->3)-alpha-d-galactopyranose residue, and terminated with alpha-d-xylopyranose r ... | 2010 | 20430053 |
| process development for mycelial growth and polysaccharide production in tricholoma matsutake liquid culture. | in this study, the effects of agitation and aeration on mycelial growth and exo-polysaccharide production were examined in batch cultures of tricholoma matsutake. agitation was varied from 100 to 300 rpm and aeration was varied from 0.5 to 1.5 vvm. mycelial growth was 21.87 g/l at 150 rpm, and exo-polysaccharide production was 8.79 g/l at 1.5 vvm. when we analyzed the polysaccharide extractions from the cultured mycelium and the culture broth of t. matsutake, 1.4 g of crude polysaccharide was fo ... | 2010 | 20226376 |
| inhibition of decrease in natural killer cell activity in repeatedly restraint-stressed mice by a biological response modifier derived from cultured mycelia of the basidiomycete tricholoma matsutake. | to develop a method to cope with stress-induced reduction in immunocompetence, we evaluated the immunomodulatory activities of a biological response modifier derived from the mycelia of the basidiomycete tricholoma matsutake (cm6271) in mice under repeated restraint stress. | 2004 | 14557678 |
| functional activation of macrophages, monocytes and splenic lymphocytes by polysaccharide fraction from tricholoma matsutake. | mushroom-derived polysaccharides (beta-glucans) are considered as a valuable biopharmaceutical principle without displaying side effects. although tricholoma matsutake is well-known mushroom in korea, japan and china, the immunoregulatory roles of t. matsutake-derived polysaccharides were not fully elucidated yet. in this study, we continued to evaluate the immunomodulatory effect of t. matsutake-derived polysaccharide fraction (tmc-2) using functional activation models of macrophages, monocytes ... | 2009 | 20091269 |
| development of specific primer for tricholoma matsutake. | in this study, in an effort to develop a method for the molecular detection of tricholoma matsutake in korea from other closely related tricholomataceae, a species-specific pcr primer pair, tmf and tmr, was designed using nuclear ribosomal intertranscribed spacer (its) sequences. the dtmf and dtmr sequences were 5'-cctgacgccaatcttttca-3' and 5'-ggagagcagacttgtgagca-3', respectively. the pcr primers reliably amplified only the its sequences of t. matsutake, and not those of other species used in ... | 2009 | 23983557 |
| forest age correlates with fine-scale spatial structure of matsutake mycorrhizas. | examining the fine-scale spatial structure of fungal populations can tell us much about how individual species reproduce and disperse throughout natural landscapes. here we study the fine-scale genetic structure of tricholoma matsutake, a prized edible and medicinal mushroom, by systematic sampling of mycorrhizas within fairy rings in 50-y-old and old-growth forests in two villages. using single nucleotide polymorphism dna markers we show that mycorrhizas in both forest age classes in both villa ... | 2009 | 19284979 |
| ectomycorrhizas in vitro between tricholoma matsutake, a basidiomycete that associates with pinaceae, and betula platyphylla var. japonica, an early-successional birch species, in cool-temperate forests. | tricholoma matsutake is an ectomycorrhizal basidiomycete that associates with pinaceae in the northern hemisphere and produces prized "matsutake" mushrooms. we questioned whether the symbiont could associate with a birch that is an early-successional species in boreal, cool-temperate, or subalpine forests. in the present study, we demonstrated that t. matsutake can form typical ectomycorrhizas with betula platyphylla var. japonica; the associations included a hartig net and a thin but distinct f ... | 2015 | 25236465 |
| the long terminal repeat (ltr) sequence of mary1, a retroelement from the ectomycorrhizal homobasidiomycete tricholoma matsutake, is highly conserved in various higher fungi. | mary1 is an ltr-retroelement from the homobasidiomycete tricholoma matsutake. nucleotide sequences that correspond to the putative u3-r region and the r-u5 region of mary1 are highly conserved in various higher fungi. data suggest that the ltr sequence of mary1 originated early in the evolution of higher fungi and has become widely distributed. therefore, it may be useful for the construction of an ltr-mediated transformation system in basidiomycetes. | 2001 | 11758926 |
| complete mitochondrial genome of the ectomycorrhizal fungus tricholoma matsutake. | tricholoma matsutake is an ectomycorrhizal fungus belonging to the class agaricomycetes and the phylum basidiomycota. here, we report the complete mitochondrial genome sequence of t. matsutake. the mitochondrial dna (mtdna) of t. matsutake seems to be concatenated, and harbors 76,037 nucleotides, with an overall gc content of 20.69%. the mtdna comprises 15 protein-coding genes, 28 trna genes, and 2 rrna genes. a comparative mitogenomic analysis between agaricomycetes and other classes (exobasidi ... | 2016 | 25208172 |
| variation among matsutake ectomycorrhizae in four clones of pinus sylvestris. | tricholoma matsutake is an ectomycorrhizal fungus that forms commercially important mushrooms in coniferous forests. in this study, we explored the ability of t. matsutake to form mycorrhizae with pinus sylvestris by inoculating emblings produced through somatic embryogenesis (se) in an aseptic culture system. two months after inoculation, clones with less phenolic compounds in the tissue culture phase formed mycorrhizae with t. matsutake, while clones containing more phenols did not. effects of ... | 2015 | 25179801 |
| immunostimulatory activities of polysaccharides from liquid culture of pine-mushroom tricholoma matsutake. | mushrooms are regarded as one of the well-known foods and biopharmaceutical materials with a great deal of interest. polysaccharide beta-glucan is the major component of mushrooms that displays various biological activities such as antidiabetic, anticancer, and antihyperlipidemic effects. in this study, we compared the immunostimulatory potency of polysaccharide fractions, prepared from liquid culture of pinemushroom tricholoma matsutake, with a potent immunogen lipopolysaccharide (lps), and the ... | 2008 | 18239423 |
| small-subunit ribosomal dna of an ectomycorrhizal fungus tricholoma matsutake: sequence, structure and phylogenetic analysis. | a 2861 bp nucleotide sequence containing tricholoma matsutake ssu rrna gene and its flanking regions was determined and analyzed. comparison with known ssu rdna sequences and primer extension analysis revealed that the ssu rrna coding region and intergenic spacer 2 (igs2) are 1805 bp and 1043 bp in length, respectively. the igs2 has an imperfect direct repeat (type 1) homologous to the region downstream to the 5s rrna gene and the three imperfect tandem direct repeats (type 2) upstream to the ss ... | 1998 | 9666460 |
| food-dependent exercise-induced anaphylaxis due to matsutake mushrooms. | | 1997 | 9205535 |
| determination of differences in the nonvolatile metabolites of pine-mushrooms (tricholoma matsutake sing.) according to different parts and heating times using 1h nmr and principal component analysis. | the differences in the nonvolatile metabolites of pine-mushrooms (tricholoma matsutake sing.) according to different parts and heating times were analyzed by applying principal component analysis (pca) to 1h nuclear magnetic resonance (nmr) spectroscopy data. the 1h nmr spectra and pca enabled the differences of nonvolatile metabolites among mushroom samples to be clearly observed. the two parts of mushrooms could be easily discriminated based on pc 1, and could be separated according to differe ... | 2007 | 18156785 |
| tricholoma matsutake 1-ocen-3-ol and methyl cinnamate repel mycophagous proisotoma minuta (collembola: insecta). | two major volatiles produced by the mycelia and fruiting bodies of tricholoma matsutake (1-octen-3-ol and methyl cinnamate) repel a mycophagous collembolan, proisotoma minuta. aggregation of the collembolans on their diet was significantly inhibited by exposure to 1 ppm methyl cinnamate or 10 to 100 ppm 1-octen-3-ol. the aggregation activity decreased dose-dependently upon exposure to 1-octen-3-ol at concentrations higher than 0.01 ppm. aggregation in the presence of methyl cinnamate exhibited t ... | 2008 | 18066606 |
| single nucleotide polymorphisms in the ectomycorrhizal mushroom tricholoma matsutake. | single nucleotide polymorphisms (snps) are becoming increasingly popular markers for studying a variety of biological phenomena. this paper describes the development and analysis of a set of snp markers for the basidiomycete fungus tricholoma matsutake. t. matsutake is a gourmet mycorrhizal mushroom primarily associated with pine forests. however, little is known about its genetics and genomic variation, including snp variation. to identify and analyse snps in t. matsutake, a genomic library was ... | 2007 | 17600046 |