| detection of an erythrovirus sequence distinct from b19 in a child with acute anaemia. | | 1998 | 9820308 |
| a novel parvovirus isolated from manchurian chipmunks. | a novel parvovirus was identified in manchurian chipmunks inhabiting korea. hepatitis b surface antigen (hbsag) was detected in sera from 4 animals among 62 apparently healthy chipmunks. electron microscopic examination of the hbsag-positive sera revealed virus-like spherical particles 20-22 nm in diameter. extraction of nucleic acid under annealing conditions from the serum samples containing virus-like particles yielded a single species of dna molecule with the electrophoretic mobility of 5.6- ... | 1999 | 9918883 |
| a quantitative, internally controlled real-time pcr assay for the detection of parvovirus b19 dna. | parvovirus b19 is an erythrovirus causing diverse clinical manifestations ranging from asymptomatic or mild, to more severe outcomes in, for example, immune-compromised patients. b19 is spread primarily via the respiratory route, but it can also be transmitted via blood and blood products. viral loads in blood or plasma donations amount up to 10(11) genome equivalents/ml. therefore, screening of plasma for fractionation for the presence of b19 and removal of highly loaded donations is a way to l ... | 2001 | 11226565 |
| persistent erythrovirus b19 urinary tract infection in an hiv-positive patient. | we report a urinary tract infection (uti) with erythrovirus b19 in an hiv-1-positive homosexual man persisting for more than 7 months after the decline of viremia after a primary infection. during the course of the uti, the patient complained of soreness in the kidney region and suffered from transient episodes of edema and hematuria. proteinuria and elevated serum concentrations of creatinine further substantiated the hypothesis of a renal focus of a persistent erythrovirus b19 infection. | 2001 | 11678936 |
| fatal myocarditis associated with acute parvovirus b19 and human herpesvirus 6 coinfection. | we report on the case of a healthy young boy who developed a fulminant myocarditis due to acute coinfection with erythrovirus (parvovirus b19) and human herpesvirus 6 (hhv-6) in the absence of an antiviral immune response. we suggest that the hhv-6-induced immunosuppression enhanced dissemination of parvovirus b19, which led to fatal myocarditis. | 2001 | 11724892 |
| red cell aplasia due to erythrovirus parvovirus b19 infection in a jehovah's witness with post-transplant ebv-induced aggressive lymphoma: management considerations. | the refusal of jehovah's witnesses to accept blood and blood products often poses a clinical dilemma to present day medicine. we present a case of a jehovah's witness who had undergone renal transplantation, only to develop an epstein-barr virus associated aggressive lymphoma post-transplant. his condition was further complicated by erythrovirus (parvovirus) b 19 infection resulting in red cell aplasia and severe anemia. the management of this difficult clinical situation is discussed together w ... | 2002 | 11911105 |
| prevalence of parvovirus b19 in liver tissue: no association with fulminant hepatitis or hepatitis-associated aplastic anemia. | parvovirus b19 has been proposed as the etiological agent of fulminant hepatitis (fh) or hepatitis-associated aplastic anemia (haa). we studied the prevalence of parvovirus b19 in liver-tissue samples from patients with fh and haa and from control subjects. in the first study, parvovirus b19 dna was detected by nested polymerase chain reaction (pcr) in 4 of 15 livers from patients with fh and in 3 of 22 livers from patients with nonviral hepatic disease. in a second confirmatory study, livers we ... | 2003 | 12721938 |
| intravenous immunoglobulins in infectious diseases: where do we stand? | intravenous immunoglobulins (ivig) are therapeutic preparations of normal human igg that have been used for more than 20 years for substitutive therapy in patients with primary antibody deficiencies. recent studies pointed out the need to obtain normal residual levels of igg (i.e. 8 g/l) in order to reduce the number and severity of bacterial infections in these patients. the ivig are also prescribed for the substitutive therapy of secondary immunodeficiencies such as chronic lymphoid leukemia a ... | 2003 | 12848745 |
| prevalence of human erythrovirus b19 dna in healthy belgian blood donors and correlation with specific antibodies against structural and non-structural viral proteins. | human parvovirus (erythrovirus) b19 is recognized as a major contaminant of blood and blood products. to reduce the risk of contamination, plasma-pool screening and exclusion of highly viraemic donations are recommended. the objectives of this study were to estimate the prevalence of b19 dna in our blood-donor population, to determine the appropriate pool size to be tested (taking into account parameters such as prevalence, viral load, test sensitivity, and the efficacy of inactivation procedure ... | 2003 | 12757504 |
| detection and differentiation of human parvovirus variants by commercial quantitative real-time pcr tests. | parvovirus b19 causes a variety of diseases in humans, with outcomes ranging from asymptomatic to severe, such as chronic anemia in immunocompromised patients or fetal hydrops and death after maternal infection during pregnancy. the virus may be transmitted via plasma-derived products. according to the results of solvent-detergent safety studies, an upper limit of b19 dna in plasma pools was recently defined. to restrict the input of b19 virus into production pools, a quantitative nucleic acid t ... | 2004 | 15131163 |
| lack of association between b19 or v9 erythrovirus infection and anca-positive vasculitides: a case-control study. | to examine the potential association of human b19 or v9 erythrovirus infection and onset of anca-positive vasculitides. | 2003 | 12709542 |
| identification of a nonconventional motif necessary for the nuclear import of the human parvovirus b19 major capsid protein (vp2). | human parvovirus b19 replicates and encapsidates its genome in the nucleus of erythroid progenitors in vivo and in vitro. we wanted to understand the determinants necessary for the nuclear transport of the major coat protein, vp2, which makes up about 96% of the viral capsid proteins. a nonconsensus basic motif, klgprkatgrw, necessary for the nuclear localization of vp2 was identified and shown to be able to import reporter proteins into the nucleus. the sequence is conserved among the vp2 c-ter ... | 2003 | 12620794 |
| analysing myocardial tissue from explanted hearts of heart transplant recipients and multi-organ donors for the presence of parvovirus b19 dna. | parvovirus b19 (pvb19) is an erythrovirus causing diverse clinical manifestations ranging from asymptomatic or mild to more severe outcomes, dependent on the haematological and/or immunological status of the host. reports of pvb19 infection as a causative agent of paediatric or adult inflammatory cardiac diseases, or of cardiac transplant rejection are rare. | 2004 | 15288611 |
| erythrovirus b19 infection in humans. | erythrovirus b19 (b19) previously called parvovirus b19 is the only human pathogen in the family parvoviridae. b19 is an autonomously replicating small single stranded non-enveloped dna of 5.5 kb with hairpin termini through which it replicates, when the cells are in the s-phase. virus host interactions are mediated through the capsid protein vp2 attaching to p antigen receptor expressed on certain host cells, which imparts narrow host and tissue tropism. it affects the progenitor red cells, meg ... | 2000 | 12452123 |
| a new parvovirus genotype persistent in human skin. | parvovirus b19 is the exclusive human pathogen of the erythrovirus genus. in classical view, the b19 dna sequence shows little variability, with no disease-specific or tissue type specific associations. we examined skin biopsies from patients with b19-unrelated skin disease or from constitutionally healthy adults by polymerase chain reaction assays for four different genomic regions of the b19 virus. sequencing showed that the skin-derived viral dna differed within the protein-coding region from ... | 2002 | 12441066 |
| identification and characterization of a second novel human erythrovirus variant, a6. | parvovirus b19 (b19), currently the only accepted member of the erythrovirus genus, is the only parvovirus known to be pathogenic in humans. recently a viral sequence, tentatively termed v9 which showed 11% variability from the published b19 sequences, was described from a patient with aplastic crisis. to search for additional parvovirus variants, we used the new ns1/7.5ec pcr assay whose primers were designed from a conserved region of the b19/v9 sequence and encompasses an mfei restriction enz ... | 2002 | 12359439 |
| splice junction map of simian parvovirus transcripts. | the transcription map of simian parvovirus (spv), an erythrovirus similar to parvovirus b19, was investigated. rna was extracted from tissues of experimentally infected cynomolgus macaques and subjected to reverse transcription-pcr with spv-specific primers. the pcr products were cloned and sequenced to identify splice junctions. a total of 14 distinct sequences were identified as putative partial transcripts. of these, 13 were spliced; a single unspliced transcript putatively encoded ns1. seque ... | 2004 | 15452211 |
| persistence of parvovirus b19 igm antibodies and dna in pure red cell aplasia resulting in myelodysplasia--a case report. | human erythrovirus b19 (b19), previously known as parvovirus b19, is a small spherical, non-enveloped single stranded dna virus. it has been shown to cause a wide spectrum of clinical conditons including various hematological disorders. we report here for the first time from inida a case of pure red cell aplasia in a 45-year-old female for last 7 years due to chronic persistent b19 infection leading to myelodysplasia after 4 years. her sera were positive for two times 4 months apart for b19 igm ... | 2004 | 15471143 |
| identification and characterization of persistent human erythrovirus infection in blood donor samples. | the presence of human erythrovirus dna in 2,440 blood donations from the united kingdom and sub-saharan africa (ghana, malawi, and south africa) was screened. sensitive qualitative and real-time quantitative pcr assays revealed a higher prevalence of persistent infection with the simultaneous presence of immunoglobulin g (igg) and viral dna (0.55 to 1.3%) than previously reported. this condition was characterized by a low viral load (median, 558 iu/ml; range, 42 to 135,000 iu/ml), antibody-compl ... | 2004 | 15507603 |
| simian parvovirus infection: a potential zoonosis. | simian parvovirus (spv) causes severe anemia in immunocompromised macaques. the closely related erythrovirus, parvovirus b19, causes anemia in susceptible humans and can be grown in human bone marrow mononuclear cells in vitro. we hypothesized that spv may infect humans and replicate in human bone marrow mononuclear cells. | 2004 | 15529252 |
| genetic diversity within human erythroviruses: identification of three genotypes. | b19 virus is a human virus belonging to the genus erythrovirus: the genetic diversity among b19 virus isolates has been reported to be very low, with less than 2% nucleotide divergence in the whole genome sequence. we have previously reported the isolation of a human erythrovirus isolate, termed v9, whose sequence was markedly distinct (>11% nucleotide divergence) from that of b19 virus. to date, the v9 isolate remains the unique representative of a new variant in the genus erythrovirus, and its ... | 2002 | 12186896 |
| evidence of serological cross-reactivity between genotype 1 and genotype 3 erythrovirus infections. | | 2005 | 15795309 |
| parvovirus b19 and erythroid cells. | parvovirus b19 is a human erythrovirus, i.e. which induces the death of erythroid progenitors. in such cells, until now only ubiquitous transcription factors have been described to regulate promoter driven gene expression. their possible interactions with erythroid specific transcription factors merit further investigations. effectively, the high level of replication of b19 in erythroid cells is not well understood. in addition to apoptosis, necrosis or inhibition of cell growth, the death of b1 ... | 2002 | 12116854 |
| parvoviruses and blood products. | erythrovirus b19 (b19), a small isocahedral, non-enveloped virus (18-26 nm), is a ubiquitous infection agent in industrialised countries. depending on the infected host, b19 has a wide range of disease manifestations from asymptomatic (the majority) to severe, including persistent infection. the risk of b19 transmission by blood products is enhanced by a high virus titre in the infected donor, by pooling of a large number of donations, and by the virus's resistance to effective inactivation meth ... | 2002 | 12116853 |
| baculovirus expression of erythrovirus v9 capsids and screening by elisa: serologic cross-reactivity with erythrovirus b19. | diagnosis of erythrovirus b19 (b19) relies on serology and the detection of viral dna. recently, a distinct erythrovirus isolate termed v9, markedly different from erythrovirus b19 (> 11% nucleotide disparity), was isolated. standard b19 pcr assays were inconclusive and serologic tests failed to categorize v9 as an acute b19-like infection. sequencing, combined with pcr studies, have since demonstrated the need for specific and differentiated techniques when examining samples for possible b19 or ... | 2002 | 11782935 |
| novel pcr assay for differential detection and screening of erythrovirus b19 and erythrovirus v9. | diagnosis of erythrovirus b19 relies on serology and the detection of viral dna. these techniques were believed to detect all field isolates because erythrovirus b19 has been known to undergo little genetic variation (1-2%). recently, a distinct erythrovirus isolate termed v9, markedly different from erythrovirus b19 (>11% nucleotide disparity), was isolated from a child in france suffering from transient aplastic anemia. standard pcr assays and serological tests failed to demonstrate an acute e ... | 2001 | 11536245 |
| parvovirus variation for disease: a difference with rna viruses? | the parvoviridae, a family of viruses with single-stranded dna genomes widely spread from invertebrates to mammal and human hosts, display a remarkable evolutionary capacity uncommon in dna genomes. parvovirus populations show high genetic heterogeneity and large population sizes resembling the quasispecies found in rna viruses. these viruses multiply in proliferating cells, causing acute, persistent or latent infections relying in the immunocompetence and developmental stage of the hosts. some ... | 2006 | 16568906 |
| reactivity of genotype-specific recombinant proteins of human erythrovirus b19 with plasmas from areas where genotype 1 or 3 is endemic. | human erythrovirus (parvovirus) b19 (b19) is a common human pathogen. the recent discovery of three genotypes, 1 to 3, raised issues related to the ability of genotype-specific antigens to cross-react with antibodies elicited by other genotypes. this study assessed antibody capture and immunoglobulin g (igg) cross-reactivity between genotype 1 and genotype 3 recombinant antigens and analyzed the potential gain of adding vp1 protein to vp2 capsid antigen. plasma samples from genotype 1- or genoty ... | 2006 | 16597864 |
| the vp1-unique region of parvovirus b19: amino acid variability and antigenic stability. | the unique region of structural protein vp1 of parvovirus b19 (erythrovirus b19) is important for eliciting neutralizing antibodies that are responsible for eliminating the virus from the peripheral blood and for inducing lifelong immunity. neutralizing human mabs bind a conformationally defined epitope spanning vp1 residues 30-42. the dna sequence encoding the vp1-unique region was determined in parvovirus b19 isolated from peripheral blood and amniotic fluid of nine acutely infected pregnant w ... | 2001 | 11125172 |
| identification of a novel rna splicing pattern as a basis of restricted cell tropism of erythrovirus b19. | prior studies on the transcription of erythrovirus b19 have identified a short leader sequence associated with all spliced viral transcripts. while some variability has been observed in the acceptor for this first intron, studies to date in both permissive and nonpermissive cell types have reported a unique splice donor site. in the semipermissive mb-02 cell line, we have found that splicing of this first intron proceeds almost exclusively via a cryptic ct donor downstream of the previously repo ... | 2000 | 10964772 |
| bioportfolio: lifelong persistence of variant and prototypic erythrovirus dna genomes in human tissue. | human erythrovirus is a minute, single-stranded dna virus causing many diseases, including erythema infectiosum, arthropathy, and fetal death. after primary infection, the viral genomes persist in solid tissues. besides the prototype, virus type 1, two major variants (virus types 2 and 3) have been identified recently, the clinical significance and epidemiology of which are mostly unknown. we examined 523 samples of skin, synovium, tonsil, or liver (birth year range, 1913-2000), and 1,640 sera, ... | 2006 | 16651522 |
| different behavior of erythrovirus b19 and torquetenovirus in response to a single step of albumin purification. | the safety of human serum albumin (hsa) is of special interest with respect to virus transmission because of the wide use of this blood product as a therapeutic agent and also, added to other products, as an excipient or a stabilizer. conflicting data are reported concerning hsa contamination by small, naked viruses such as the erythrovirus b19 (b19v) and the anellovirus torquetenovirus (ttv). this study has been performed to assess the effect of the hsa purification procedures on the viral cont ... | 2006 | 16836563 |
| haematological consequences of parvovirus b19 infection. | parvovirus b19, a member of the erythrovirus genus, is the only member of the parvoviridae family known to be pathogenic in humans. erythroviruses are so named because of their tropism and selective replication in erythroid progenitor cells. haematological consequences of b19 infection arise due to a direct cytotoxic effect on erythroid progenitors in bone marrow with interruption of erythrocyte production. in addition, the physiology of host haematopoiesis and competence of the immune response ... | 2000 | 10942624 |
| erythroviruses as gene transfer vehicles. | | 2000 | 10941575 |
| effects of transfusion on human erythrovirus b19-susceptible or -infected pediatric recipients in a genotype 3-endemic area. | human erythrovirus (parvovirus) b19 is transmitted by transfusion of blood, blood components, and plasma derivatives and is resistant to most viral inactivation methods. b19 genotype 3 is prevalent in ghana, and no related clinical information is available. | 2006 | 16965589 |
| molecular biology of erythroviruses. | | 2000 | 10941574 |
| epidemiology and pathology of erythroviruses. | | 2000 | 10941573 |
| rhesus and pig-tailed macaque parvoviruses: identification of two new members of the erythrovirus genus in monkeys. | we have previously reported the identification of a novel simian parvovirus in cynomolgus monkeys, which causes severe anemia in immunosuppressed cynomolgus monkeys and is currently being studied as an animal model for human b19 infection. we now report two similar outbreaks of anemia in rhesus and pig-tailed macaques associated with two distinct but similar simian parvoviruses (pig-tailed macaque and rhesus parvovirus). both viruses have been cloned and over 5000 nucleotides sequenced from each ... | 2000 | 10725203 |
| inactivation of parvovirus b19 in human platelet concentrates by treatment with amotosalen and ultraviolet a illumination. | the human erythrovirus b19 (b19) is a small (18- to 26-nm) nonenveloped virus with a single-stranded dna genome of 5.6 kb. b19 is clinically significant and is also generally resistant to pathogen inactivation methods. photochemical treatment (pct) with amotosalen and ultraviolet a (uva) inactivates viruses, bacteria, and protozoa in platelets (plts) and plasma prepared for transfusion. in this study, the capacity of pct to inactivate b19 in human plt concentrates was evaluated. | 2007 | 17524098 |
| novel human erythrovirus associated with transient aplastic anemia. | erythrovirus (formerly parvovirus) b19 causes a wide range of diseases in humans, including anemia due to aplastic crisis. diagnosis of b19 infection relies on serology and the detection of viral dna by pcr. these techniques are usually thought to detect all erythrovirus field isolates, since the b19 genome is known to undergo few genetic variations. we have detected an erythrovirus (v9) markedly different from b19 in the serum and bone marrow of a child with transient aplastic anemia. the b19 p ... | 1999 | 10405389 |
| the simian parvoviruses. | autonomous parvoviruses with tropism for erythroid cells have recently been reclassified in a new genus, erythrovirus. although b19 is the type member, and presently the only internationally accepted member of the erythrovirus genus, we have identified three new simian viruses, all of which have the molecular features of parvoviruses, and are highly tropic for erythroid progenitor cells. this review describes the identification of these new animal parvoviruses and summarises current knowledge of ... | 1997 | 10398485 |
| cloning and sequencing of the simian parvovirus genome. | we recently reported the identification of a novel simian parvovirus in cynomolgus monkeys with severe anemia. we now describe the cloning and sequencing of 4986 nucleotides of the viral dna. like the human parvovirus b19, simian parvovirus encapsidates both positive and negative single-stranded dna. the positive strand contains two large open reading frames, with the left open reading frame encoding the nonstructural protein(s) and the right reading frame encoding the two capsid proteins. simia ... | 1995 | 7618269 |
| continuous-flow uvc irradiation: a new, effective, protein activity-preserving system for inactivating bacteria and viruses, including erythrovirus b19. | despite the increasing number of screening tests being introduced, ensuring the inactivation of blood-borne pathogens in blood-derived therapeutic material is a major concern. dynamic continuous-flow uvc irradiation is a new way to inactivate a large range of pathogens without adding any photosentizers. the efficacy of different methods was evaluated against the following viruses: murine parvovirus mvmp, human b19, the encephalomyocarditis virus (emc, a picornavirus used as a model for model for ... | 2004 | 15081608 |
| viremic blood donor found by a rapid screening method in a season of high human parvovirus b19 activity in niterói, rio de janeiro, brazil. | erythrovirus b19 infection is usually benign but may have serious consequences in patients with hemolytic anemia (transient aplastic crisis), immunodeficiency (in whom persistent infection can lead to chronic bone marrow failure with anemia), or who are in the first or second trimester of gestation (spontaneous abortion, hydrops fetalis, and fetal death). being non-enveloped, b19 resists most inactivation methods and can be transmitted by transfusion. b19 is difficult to cultivate and native vir ... | 2004 | 15057355 |
| [genetic diversity of human erythroviruses]. | b19 parvovirus (b19v) has been considered for a long period of time as the unique human virus belonging to the genus erythrovirus. the genetic diversity of b19v isolates has been shown to be very low (<2% nucleotide divergence). the isolation of a variant (v9 strain), with a sequence markedly distinct from that of b19v (>13% nucleotide divergence) led to specify the classification of this virus family. phylogenetic analysis of partial sequences of v9-related isolates combined with erythrovirus s ... | 2009 | 18387751 |
| lightcycler consensus pcr for rapid and differential detection of human erythrovirus b19 and v9 isolates. | until recently, b19 virus was considered to be the only human pathogen of the genus erythrovirus. however, other non-b19 virus strains, such as v9, have now been isolated and are thought to cause infections clinically and serologically indistinguishable from those caused by b19 virus. whereas b19 virus related isolates have a low genetic diversity of only 1-2%, nucleotide disparity of up to 12% was found for the new isolates, suggesting that non-b19 virus isolates may not be detectable using b19 ... | 2004 | 15042648 |
| seroepidemiology of human bocavirus defined using recombinant virus-like particles. | human bocavirus (hbov) is a newly identified human parvovirus for which seroepidemiology and antigenic properties remain undefined. | 2008 | 18491974 |
| b19 virus genome diversity: epidemiological and clinical correlations. | genetic analysis of parvovirus b19 has been carried out mainly to establish a framework to track molecular epidemiology of the virus and to correlate sequence variability with different pathological and clinical manifestations of the virus. a good amount of information regarding b19 virus sequence variability is available, and presently there are about 400 sequence records deposited in the nucleotide database of ncbi. a few are almost complete genomic sequences, and these allow the construction ... | 2003 | 12927746 |
| advances in the biology, diagnosis and host-pathogen interactions of parvovirus b19. | increased recognition of parvovirus b19 (b19), an erythrovirus, as a significant human pathogen that causes fetal loss and severe disease in immunocompromised patients has resulted in intensive efforts to understand the pathogenesis of b19-related disease, to improve diagnostic strategy that is deployed to detect b19 infection and blood-product contamination and, finally, to elucidate the nature of the cellular immune response that is elicited by the virus in diverse patient cohorts. it is becom ... | 2004 | 15150324 |
| different susceptibility of b19 virus and mice minute virus to low ph treatment. | parvoviridae are small nonenveloped viruses that are known to be highly resistant to physico-chemical treatments. because low ph is frequently applied to process intermediates or final products, the impact of such conditions on the human erythrovirus b19 (b19v) and the mouse parvovirus (mice minute virus, mmv) was assessed, which is often used as a model for b19v. owing to the lack of a suitable cultivation and/or detection system for b19v no such data exist so far. | 2004 | 15225251 |
| the structure of human parvovirus b19. | human parvovirus b19 is the only parvovirus known to be a human pathogen. the structure of recombinant b19-like particles has been determined to approximately 3.5-a resolution by x-ray crystallography and, to our knowledge, represents the first near-atomic structure of an erythrovirus. the polypeptide fold of the major capsid protein vp2 is a "jelly roll" with a beta-barrel motif similar to that found in many icosahedral viruses. the large loops connecting the strands of the beta-barrel form sur ... | 2004 | 15289612 |
| hypoxia enhances human b19 erythrovirus gene expression in primary erythroid cells. | human b19 erythrovirus replicates in erythroid progenitors present in bone marrow and fetal tissues where partial oxygen tension is low. here we show that infected human primary erythroid progenitor cells exposed to hypoxia (1% o2) in vitro increase viral capsid protein synthesis, virus replication, and virus production. hypoxia-inducible factor-1 (hif-1), the main transcription factor involved in the cellular response to reduced oxygenation, is shown to bind an hif binding site (hbs) located in ... | 2004 | 15327892 |
| simian parvoviruses: biology and implications for research. | the simian parvoviruses (spvs) are in the genus erythrovirus in the family parvoviridae and are most closely related to the human virus b19. spv has been identified in cynomolgus, rhesus, and pigtailed macaques. all of the primate erythroviruses have a predilection for erythroid precursors. infection, which is common in macaques, is usually clinically silent. disease from spv is associated with immunosuppression due to infection with various retroviruses (siv, simian retrovirus, and simian-human ... | 2008 | 19793456 |
| hypoxia enables b19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line. | b19 may cause mild to severe clinical manifestations. owing to the remarkable tropism of b19 for red blood cell progenitors, there is a lack of satisfactory cell lines fully permissive for b19. because the local oxygen pressure may influence viral replication, we used hypoxia to improve the sensitivity of our infectivity assay in order to link b19 dna detected by pcr to the presence of infectious b19 particles in plasma. plasma samples and the who international standard for b19 dna detection by ... | 2004 | 15381351 |
| comparison of the transcription profile of simian parvovirus with that of the human erythrovirus b19 reveals a number of unique features. | simian parvovirus (spv) is a member of the genus erythrovirus and is closely related to the human parvovirus b19. natural and experimental infection of monkeys with spv resembles b19 infection of human. we report a detailed characterization of the viral rnas and proteins generated following transfection of cloned spv into cos cells and spv infection of the human erythroid progenitor line ut-7/epo-s1. spv and b19 are 50% identical at the nucleotide level, and although their basic transcription an ... | 2004 | 15542645 |
| human parvovirus b19: a new emerging pathogen of inflammatory cardiomyopathy. | the human parvovirus b19 (pvb19), an erythrovirus causing diverse clinical manifestations ranging from asymptomatic or mild to more severe outcomes such as hydrops fetalis, is the only known human pathogenic parvovirus so far. although enteroviruses have long been considered the most common cause of inflammatory cardiomyopathy, pvb19 is emerging as a important candidate. recent studies have indicated an association of pvb19 with paediatric and adult inflammatory cardiac disease. however, whether ... | 2005 | 16316397 |
| human bocavirus capsid structure: insights into the structural repertoire of the parvoviridae. | human bocavirus (hbov) was recently discovered and classified in the bocavirus genus (family parvoviridae, subfamily parvovirinae) on the basis of genomic similarity to bovine parvovirus and canine minute virus. hbov has been implicated in respiratory tract infections and gastroenteric disease in children worldwide, yet despite numerous epidemiological reports, there has been limited biochemical and molecular characterization of the virus. reported here is the three-dimensional structure of reco ... | 2010 | 20375175 |
| parvovirus b19: a new emerging pathogenic agent of inflammatory cardiomyopathy. | the human parvovirus b19 (pvb19), an erythrovirus causing diverse clinical manifestations ranging from asymptomatic or mild to more severe outcomes such as hydrops fetalis, is the only currently known human pathogenic parvovirus. recently, pvb19 has been identified as a causative agent of pediatric and adult inflammatory cardiac diseases. the first hints for a possible etiopathogenetic role of the pvb19 infection and the development of cardiac dysfunction were demonstrated by molecular biology m ... | 2006 | 16329659 |
| sequence variability of human erythroviruses present in bone marrow of brazilian patients with various parvovirus b19-related hematological symptoms. | the presence of erythrovirus infections was investigated by pcr with bone marrow samples of patients with various parvovirus b19-related hematological symptoms. erythrovirus dna was found in 17.3% (12/69) of patients. phylogenetic analysis revealed that five strains cluster with genotype 1, one clusters with genotype 2, and six cluster with genotype 3. our study is the first to document the presence of the three erythrovirus genotypes in brazil. | 2006 | 16455922 |
| phylogenetic evidence for the rapid evolution of human b19 erythrovirus. | human b19 erythrovirus is a ubiquitous viral pathogen, commonly infecting individuals before adulthood. as with all autonomous parvoviruses, its small single-stranded dna genome is replicated with host cell machinery. while the mechanism of parvovirus genome replication has been studied in detail, the rate at which b19 virus evolves is unknown. by inferring the phylogenetic history and evolutionary dynamics of temporally sampled b19 sequences, we observed a surprisingly high rate of evolutionary ... | 2006 | 16537636 |
| hypoxic-response elements in the oncolytic parvovirus minute virus of mice do not allow for increased vector production at low oxygen concentration. | vectors derived from the autonomous parvovirus minute virus of mice, mvm(p), are promising tools for the gene therapy of cancer. the validation of their in vivo anti-tumour effect is, however, hampered by the difficulty to produce high-titre stocks. in an attempt to increase vector titres, host cells were subjected to low oxygen tension (hypoxia). it has been shown that a number of viruses are produced at higher titres under these conditions. this is the case, among others, for another member of ... | 2006 | 16603521 |
| simian parvovirus infection in cynomolgus monkey heart transplant recipients causes death related to severe anemia. | simian parvovirus (spv) was first isolated from cynomolgus monkeys. like human parvovirus b19, this virus has a predilection for erythroid cells. during acute spv infection, clinical signs are usually mild or inapparent, but severe anemia may occur in immunocompromised animals. we report several cases of symptomatic spv infection in cynomolgus monkeys following heart transplantation. | 2006 | 16641603 |
| the prevalence of human parvovirus b19 dna and antibodies in blood donors from four chinese blood centers. | background: human parvovirus b19 is a common human pathogen that causes a variety of diseases with outcomes ranging from asymptomatic to severe, especially in immunocompromised patients. the b19 virus can be transmitted via blood and/or blood products and its resistance to common viral inactivation and/or removal methods raises the importance of b19-related blood safety. however, the existence, variation, and loading of b19 in chinese blood donors have not been determined. study design and metho ... | 2011 | 21382040 |
| [emergent viral threats in blood transfusion]. | during the last 20 years, the safety of blood products increased dramatically with regard to the infectious risk and notably to that represented by retroviruses (hiv and htlv) and hepatitis b and c viruses. the aim of this review is to identify the residual and emergent viral threats that could be responsible for the occurring of new contaminations in the receivers of blood products. beside many other viruses (hhv-8, erythrovirus b19, hepatitis a and e viruses...), a special attention has been p ... | 2011 | 21414828 |
| human parvovirus b19: general considerations and impact on patients with sickle cell disease and thalassemia as well as on blood transfusions. | human parvovirus b19 (b19v) is a small (22-24 nm) non-enveloped dna virus, belonging to the genus erythrovirus (family parvoviridae). although, it causes generally self-limiting conditions in healthy people, b19v infection could have different outcome in patients with inherited hemolytic anemias. in such high-risk individuals, the high-titer replication may result in bone marrow suppression triggering life-threatening drop of the hemoglobin values (profound anemia, aplastic crisis). nevertheless ... | 2011 | 21585562 |
| vasculitides secondary to infections. | many viruses can be responsible for systemic vasculitis, the most frequent being hepatitis b virus-related polyarteritis nodosa (hbv-pan), even though its incidence has decreased over the past few decades. mixed cryoglobulinemia has been shown to be associated with hepatitis c virus (hcv) infection in more than 80% of the patients, but it remains asymptomatic in most of them with only a minority developing vasculitis. human immunodeficiency virus (hiv), erythrovirus b19, cytomegalovirus, varicel ... | 2006 | 16859600 |
| erythrovirus b19 infection in acquired immunodeficiency syndrome: screening by histopathology, immunohistochemistry, and in situ hybridization. | erythrovirus b19 infects erythrocytic progenitors, transiently interrupting erythropoiesis. in aids patients it causes chronic anemia amenable to treatment. we looked for evidences of b19 infection in stored bone marrow material from patients with acquired immunodeficiency syndrome. histological sections were made from stored paraffin blocks from 33 autopsies (39 blocks) and 35 biopsies (45 blocks, 30 patients) performed from 1988 to 2002. they were examined after hematoxylin-eosin (he) staining ... | 2006 | 16951812 |
| human parvovirus b19 erythrovirus. methods established for virological and diagnostic aspects. | | 2006 | 17010169 |
| [erythrovirus b19 infection]. | erythrovirus b19 has been associated with an expanding range of clinical disorders since its identification as the etiological agent of erythema infectiosum, or fifth disease of childhood: acute arthropathy, dermatologic manifestations, chronic anemia in immunocompromised patients, and transient aplastic crisis in individuals with underlying chronic hemolytic disorders. furthermore, exposure to and infection by b19 virus can lead to serious complications during pregnancy, which may result in fet ... | 2006 | 17125665 |
| characterization of erythrovirus b19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation. | fulminant hepatitis and biliary atresia are serious problems and their causes have not been explained well. we investigated whether or not erythrovirus b19 is a candidate etiologic agent in such liver disease patients who had undergone liver transplantation. | 2007 | 17479159 |
| human erythrovirus b19 and blood transfusion - an update. | erythrovirus (parvovirus) b19 (b19) is a common human pathogen. it is a non-enveloped single-strand dna virus packaging its genome in small tight capsids consisting of viral vp1 and vp2 proteins. it is now accepted that b19 is a relatively quickly evolving virus having diverged in several genetic variants recently identified. the main route of b19 transmission is respiratory, with a majority of infections occurring during childhood and manifesting as erythema infectiousum. b19 can also be transm ... | 2007 | 17680952 |
| the "pressure pan" evolution of human erythrovirus b19 in the amazon, brazil. | to understand the evolutionary dynamics of human parvovirus b19, we analyzed vp1 and vp2 gene sequences of b19 sampled from belém (amazon), the city of são paulo, brazil and globally. our analysis revealed a strikingly different pattern of evolutionary change for those viral lineages introduced into belém, which exhibited a higher rate of nonsynonymous substitutions compared to those viruses sampled from other locations. we propose that difference this is due to the high prevalence of b19 in bel ... | 2007 | 17698162 |
| heteroduplex mobility assay and single-stranded conformation polymorphism analysis as methodologies for detecting variants of human erythroviruses. | variant samples from the three genotypes of erythroviruses have already been detected using sequencing as methodology for analysis. this study aimed to investigate the efficacy of single-stranded conformation polymorphism (sscp) analysis and heteroduplex mobility assay (hma) as methodologies to detect human erythrovirus variants, using their vp1 unique region sequences. clinical samples and plasmids of pvbaua, a6, lali, v9gh3051, and d91.1 erythrovirus variants as prototypes of the three genotyp ... | 2008 | 18054089 |
| prevalence of erythrovirus genotypes in the myocardium of patients with dilated cardiomyopathy. | parvovirus b19 (pvb19) is a member of the human erythrovirus family detected frequently in endomyocardial biopsies from patients with dilated cardiomyopathy. human erythroviruses cluster into three genotypes 1-3 which share a high degree of homology between major structural proteins and may cause indistinguishable infections clinically and serologically. in human cardiac tissue erythrovirus genotypes other than pvb19 have not yet been reported. three hundred seventeen consecutive patients with s ... | 2008 | 18461615 |
| molecular characterization of human erythrovirus b19 strains obtained from patients with several clinical presentations in the amazon region of brazil. | human erythrovirus b19, endemic in the amazon region since 1990, is associated with a wide spectrum of clinical presentations. | 2008 | 18502682 |
| human b19 erythrovirus in vitro replication: what's new? | | 2008 | 18703540 |
| seroprevalence of erythrovirus b19 igg antibody among paediatric patients in makkah and jeddah, kingdom of saudi arabia. | to estimate the prevalence of igg antibodies against b19 virus (b19v) in makkah and jeddah, saudi arabia. | 2009 | 19494546 |
| the genome of human parvovirus b19 can replicate in nonpermissive cells with the help of adenovirus genes and produces infectious virus. | human parvovirus b19 (b19v) is a member of the genus erythrovirus in the family parvoviridae. in vitro, autonomous b19v replication is limited to human erythroid progenitor cells and in a small number of erythropoietin-dependent human megakaryoblastoid and erythroid leukemic cell lines. here we report that the failure of b19v dna replication in nonpermissive 293 cells can be overcome by adenovirus infection. more specifically, the replication of b19v dna in the 293 cells and the production of in ... | 2009 | 19587029 |
| phylogenetic analysis of a near-full-length sequence of an erythrovirus genotype 3 strain isolated in brazil. | human parvovirus b19 is the only member of the genus erythrovirus that causes human disease. recent findings of several strains with considerable sequence divergence from b19 have suggested a new classification for parvovirus genotypes as 1 (b19), 2 (a-6 and lali) and 3 (v9). in their overall dna sequence, the three genotypes differ by approximately 10%. here, we report the isolation of a genotype-3-related strain named br543 during a prospective study conducted in sao paulo, brazil. analysis of ... | 2009 | 19701602 |
| [genetic diversity of human erythroviruses. consequences on infectious safety of plasma derivatives]. | the b19 parvovirus (b19v) has for a long time been considered as the unique human virus belonging to the genus erythrovirus. the genetic diversity of b19v isolates has been shown to be very low. the isolation of a variant (v9 strain), with a sequence markedly distinct from that of b19v which led to attributing this classification to this family of viruses. phylogenetic analysis of sequences of v9-related isolates indicates an organization into three well-individualized genotypes. the b19v infect ... | 2009 | 19880339 |
| infrequent replication of parvovirus b19 and erythrovirus genotypes 2 and 3 among hiv-infected patients with chronic anemia. | we investigated the role that erythroviruses (parvovirus b19 and erythrovirus genotypes 2 and 3) play in the lives of immunosuppressed hiv-infected patients with chronic anemia. we screened the serum samples of 428 patients by specific ultrasensitive real-time polymerase chain reaction assay. sixteen patients had circulating dna, with no apparent clinical impact. erythrovirus-associated anemia is an extremely rare event in hiv-infected patients. | 2010 | 19951110 |
| respiratory viruses, a common microbiological finding in neutropenic children with fever. | febrile neutropenia is a common complication in children undergoing chemotherapy for malignancies. a microbial agent is only identified in 15-30% of the fever episodes and corresponds mostly to bacterial findings. | 2010 | 20056482 |
| advances in human b19 erythrovirus biology. | since its discovery, human parvovirus b19 (b19v), now termed erythrovirus, has been associated with many clinical situations (neurological and myocardium infections, persistent b19v dnaemia) in addition to the prototype clinical manifestations, i.e., erythema infectiosum and erythroblastopenia crisis. in 2002, the use of new molecular tools led to the characterization of three different genotypes of human b19 erythrovirus. although the genomic organization is conserved, the geographic distributi ... | 2010 | 20631151 |
| widespread infection with homologues of human parvoviruses b19, parv4, and human bocavirus of chimpanzees and gorillas in the wild. | infections with human parvoviruses b19 and recently discovered human bocaviruses (hbovs) are widespread, while parv4 infections are transmitted parenterally and prevalent specifically in injecting drug users and hemophiliacs. to investigate the exposure and circulation of parvoviruses related to b19 virus, parv4, and hbov in nonhuman primates, plasma samples collected from 73 cameroonian wild-caught chimpanzees and gorillas and 91 old world monkey (owm) species were screened for antibodies to re ... | 2010 | 20668071 |
| pregnancy loss ascribable to parvovirus b19/erythrovirus is associated with a high prevalence of trisomy. | in most cases of stillbirth, the cause is still unknown. | 2010 | 21051853 |
| the detection of parvoviruses. | parvovirus b19 is a single-stranded dna virus which causes severe disease in immunocompromised patients and foetal loss in pregnant women. it is classified as an erythrovirus and this genus also comprises two related viral genotypes (so-called lali/a6 (genotype 2) and v9 (genotype 3)) which appear to be immunologically indistinguishable from parvovirus b19. serological and nucleic acid test (nat) systems to detect parvovirus b19-mediated infection are commercially available; however, some nat sy ... | 2011 | 21116804 |
| chipmunk parvovirus is distinct from members in the genus erythrovirus of the family parvoviridae. | the transcription profile of chipmunk parvovirus (chppv), a tentative member of the genus erythrovirus in the subfamily parvovirinae of the family parvoviridae, was characterized by transfecting a nearly full-length genome. we found that it is unique from the profiles of human parvovirus b19 and simian parvovirus, the members in the genus erythrovirus so far characterized, in that the small rna transcripts were not processed for encoding small non-structural proteins. however, like the large non ... | 2010 | 21151930 |
| [parvovirus b19: importance of diathesis in the clinical expression of a common infection]. | symptomatic manifestations of parvovirus b19 infection range from harmless conditions such as 5th disease of the child or arthropathy of the middle-aged woman to life threatening disease such as transient aplastic crisis during sickle cell disease, chronic anaemia in immunodeficiency states or hydrops foetalis during pregnancy. increasing knowledge of parvovirus b19 has led to a better understanding about how a single and unvariant erythrovirus causes such a variety of diseases. the importance o ... | 2007 | 17844796 |
| improved detection of acute parvovirus b19 infection by immunoglobulin m eia in combination with a novel antigen eia. | although parvovirus b19 is a significant blood product contaminant, few methods other than polymerase chain reaction (pcr) have been developed to detect the presence of the virus. | 2007 | 17845258 |