| comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groups. | the dna and putative amino acid sequences of representative insect and shrimp parvoviruses (subfamily densovirinae) were analyzed using computer programs. shrimp viruses included hepatopancreatic parvovirus (hpv) of penaeus monodon (hpvmon) and p. chinensis (hpvchin), spawner-isolated mortality virus from p. monodon (smvmon) and infectious hypodermal and hematopoietic necrosis virus (ihhnv) from p. vannamei. insect viruses included aedes aegypti densovirus (aaednv), aedes albopictus densovirus ( ... | 2002 | 12135792 |
| trypsin inhibitor from poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases. | plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves of being attacked by insects. in this work, a novel trypsin inhibitor (ppti) was purified from the seeds of the native brazilian tree poecilanthe parviflora (benth) (papilioinodeae, leguminosae) by gel filtration chromatography on a sephadex g-100 followed by superdex g75 chromatography (fplc), sepharose 4b-trypsin column, and fractionated by reversed-phase hplc on a c-18 column. sds-pag ... | 2004 | 15328890 |
| [natural presence of beauveria bassiana (balsamo) vuillemin in the sugar cane (saccharum sp. hybrid) in cuba]. | the natural presence of beauveria bassiana was determined (balsamo) vuillemin from insects with mycotic symptoms collected in leaves and in the stalks of sugar cane (saccharum sp. hybrid) in pinar del río, ciudad de la habana, habana, matanzas, villa clara, cienfuegos and camagüey. the results obtained demonstrate the natural presence of the entomopathogen fungus in larvae and chrysalises of diatraea saccharalis (fabricius) and they suggest the basis for the strategy of biological control agains ... | 2004 | 15458363 |
| management of feeding damage and survival of southwestern corn borer and sugarcane borer (lepidoptera: crambidae) with bacillus thuringiensis transgenic field corn. | the efficacy of transgenic corn hybrids expressing an insecticidal bacillus thuringiensis (bt) delta-endotoxin from different transformation events was evaluated in field corn, zea mays l., against the southwestern corn borer, diatraea grandiosella dyar, and sugarcane borer, diatraea saccharalis (f.). susceptibilities of neonates and third instars were determined on bt and non-bt corn plants (v6 and r1 stages) in field plots and corn leaf tissue feeding exposure in laboratory bioassays. bt corn ... | 2004 | 15666772 |
| absorption of toxic beta-glucosides produced by plants and their effect on tissue trehalases from insects. | trehalases present in body wall, malpighian tubules, fat body, midgut and haemolymph from tenebrio molitor (coleoptera), musca domestica (diptera), spodoptera frugiperda and diatraea saccharalis (lepidoptera) were assayed in the presence and absence of toxic beta-glucosides produced by plants or their aglycones. the glucosides used were phlorizin, amygdalin, prunasin and the aglycone mandelonitrile. in addition, t. molitor and s. frugiperda trehalases were assayed with and without esculin. more ... | 2006 | 16458033 |
| interactions between isolates of the entomopathogenic fungus metarhizium anisopliae and the entomopathogenic nematode heterorhabditis bacteriophora jpm4 during infection of the sugar cane borer diatraea saccharalis (lepidoptera: pyralidae). | interactions between the nematode heterorhabditis bacteriophora isolate jpm4 and the fungus metarhizium anisopliae, isolates lpp45 and lpp39, were studied during dual infections of diatraea saccharalis. mortality, production of infective juveniles (ijs) and production of conidia were evaluated. a positive effect was demonstrated for host mortality in duel infections of jpm4 and lpp39, causing 100% mortality with lt(50) and lt(95) values of 1.8 and 2.8 days, respectively. higher values were seen ... | 2007 | 17532003 |
| [artificial diet for rearing doru luteipes (scudder) (dermaptera: forficulidae), a predator of the fall armyworm, spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae)]. | a new technique involving an artificial diet and an artificial substrate for oviposition for the rearing of the predator doru luteipes (scudder) is suggested. both adults and nymphs were maintained in petri dishes containing a transparent piece of soda straw filled with moistened cotton and the corresponding food for the biossays. the following treatments were tested: eggs of spodoptera frugiperda (j.e. smith), diatraea saccharalis fabricius (lepidoptera: pyralidae) and anagasta kuehniella zelle ... | 2007 | 17607467 |
| f2 screen for resistance to a bacillus thuringiensis-maize hybrid in the sugarcane borer (lepidoptera: crambidae). | a novel f2 screening technique was developed for detecting resistance in sugarcane borer, diatraea saccharalis (f.), to transgenic bacillus thuringiensis (bt)-maize expressing the cry1ab insecticidal protein. the f2 screening method involved (i) collecting larvae from maize fields; (ii) establishing two-parent families; (iii) screening f2 neonates for survival on bt-maize leaf tissues; and (iv) confirming resistance on commercial bt-maize plants. with the f2 screening method, 213 iso-line famili ... | 2007 | 17916262 |
| susceptibility of cry1ab-resistant and -susceptible sugarcane borer (lepidoptera: crambidae) to four bacillus thuringiensis toxins. | sugarcane borer, diatraea saccharalis (f.), is a primary corn stalk borer pest targeted by transgenic corn expressing bacillus thuringiensis (bt) proteins in many areas of the mid-southern region of the united states. recently, genes encoding for cry1a.105 and cry2ab2 bt proteins were transferred into corn plants (event mon 89034) for controlling lepidopteran pests. this new generation of bt corn with stacked-genes of cry1a.105 and cry2ab2 will become commercially available in 2009. susceptibili ... | 2009 | 18955062 |
| purification and characterization of a trypsin inhibitor from plathymenia foliolosa seeds. | a novel trypsin inhibitor (pfti) was isolated from plathymenia foliolosa (benth.) seeds by gel filtration chromatography on a sephadex g-100, deae-sepharose, and trypsin-sepharose columns. by sdspage, pfti yielded a single band with a m(r) of 19 kda. pfti inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants (k(i)) of 4 x 10(-8) and 1.4 x 10(-6) m, respectively. pfti retained more than 50% of activity at up to 50 degrees c for 30 min, but there were 80 and 100% ... | 2008 | 18991455 |
| inheritance of resistance to bacillus thuringiensis cry1ab protein in the sugarcane borer (lepidoptera: crambidae). | inheritance traits of a cry1ab-resistant strain of the sugarcane borer, diatraea saccharalis (f.) were analyzed using various genetic crosses. reciprocal parental crosses between cry1ab-susceptible and cry1ab-resistant populations, f(1) by f(1) crosses, and backcrosses of f(1) with the cry1ab-resistant population were successfully completed. larval mortality of the parental and cross-populations were assayed on cry1ab diet and bacillus thuringiensis (bt)-corn leaf tissue. maternal effects and se ... | 2009 | 19527726 |
| toxicity of hydrolyzed vicilins toward callosobruchus maculatus and phytopathogenic fungi. | studies have shown that vicilins (7s storage proteins) from seeds were able to bind to the surface of the callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of diatraea saccharalis and tenebrio molitor , inhibiting larval development. vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. the present work studies the association of peptides from vicilins of genotypes of vigna unguicula ... | 2009 | 19658384 |
| molecular characterization and rna interference of three midgut aminopeptidase n isozymes from bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, diatraea saccharalis. | aminopeptidase n (apn) proteins located at the midgut epithelium of some lepidopteran species have been implicated as receptors for insecticidal proteins from bacillus thuringiensis. cdnas of three apn isoforms, dsapn1, dsapn2, and dsapn3, from cry1ab-susceptible (cry1ab-ss) and -resistant (cry1ab-rr) strains of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), were identified and sequenced using reverse transcriptase polymerase chain reaction (rt-pcr) and 5' rapid amplifi ... | 2010 | 20685334 |
| laboratory and field tests of spray-dried and granular formulations of a bacillus thuringiensis strain with insecticidal activity against the sugarcane borer. | formulations of bacillus thuringiensis berliner (bt) with insecticidal activity against the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: pyralidae), were developed and tested under laboratory and field conditions. the formulations were prepared using biodegradable polymers such as modified corn starch as an encapsulating agent, gelatin as an adherent, powdered sugarcane as a feeding stimulant and a bt var. kurstaki gm-34 strain from a non-sugarcane region as the active ingredien ... | 2006 | 16786544 |
| comparative susceptibility of european corn borer, southwestern corn borer, and sugarcane borer (lepidoptera: crambidae) to cry1ab protein in a commercial bacillus thuringiensis corn hybrid. | one field strain each of the european corn borer, ostrinia nubilalis (hübner); southwestern corn borer, diatraea grandiosella dyar; and sugarcane borer, diatraea saccharalis (f.); were collected from cornfields in northeastern louisiana. susceptibilities of the field strain and a corresponding laboratory strain of the three borer species to cry1ab protein in dk69-70 bacillus thuringiensis (bt) corn hybrid were determined by exposing neonates to intact leaf tissues from whorl stage plants or by f ... | 2006 | 16573340 |
| down regulation of a gene for cadherin, but not alkaline phosphatase, associated with cry1ab resistance in the sugarcane borer diatraea saccharalis. | the sugarcane borer, diatraea saccharalis, is a major target pest of transgenic corn expressing bacillus thuringiensis (bt) proteins (i.e., cry1ab) in south america and the mid-southern region of the united states. evolution of insecticide resistance in such target pests is a major threat to the durability of transgenic bt crops. understanding the pests' resistance mechanisms will facilitate development of effective strategies for delaying or countering resistance. alterations in expression of c ... | 2011 | 21991350 |
| host refractoriness of the tobacco hornworm, manduca sexta, to the braconid endoparasitoid cotesia flavipes. | cotesia flavipes (hymenoptera:braconidae) is a gregarious endoparasitoid of several pyralid stemborer larvae of economic significance including the sugarcane borer, diatraea saccharalis. in this study, the ability of this parasitoid to develop in a sphingid host, manduca sexta, was tested. first, second, third, fourth, and even pharate fifth instar host tobacco hornworm larvae were readily parasitized by the female c. flavipes parasitoids but no wasp larvae hatched from the eggs in this refracto ... | 2005 | 16304618 |
| genetic relationships between resistance to stalk-tunneling by the european corn borer and cell-wall components in maize population b73xb52. | the objective of this study was to assess the relationships among quantitative trait loci (qtl) detected for european corn borer (ecb) tunneling and cell-wall components (cwc) neutral detergent fiber (ndf), acid detergent fiber (adf), and acid detergent lignin (adl) content in leaf-sheath and stalk tissues in a maize recombinant inbred line population derived from inbred lines b73 and b52. most of the qtl for ecb resistance (10/13) were at qtl positions for one or more cwc. of the 12 qtl for ndf ... | 2005 | 15887040 |
| isolation of cellulolytic bacteria from the intestine of diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass. | as a strategy to find efficient lignocellulose degrading enzymes/microorganisms for sugarcane biomass pretreatment purposes, 118 culturable bacterial strains were isolated from intestines of sugarcane-fed larvae of the moth diatraea saccharalis. all strains were tested for cellulolytic activity using soluble carboxymethyl cellulose (cmc) degrading assays or by growing bacteria on sugarcane biomass as sole carbon sources. out of the 118 strains isolated thirty eight were found to possess cellulos ... | 2015 | 25852992 |
| the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi. | plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ... | 2014 | 24608349 |
| laboratory and field evaluation of beauveria bassiana against sugarcane stalkborers (lepidoptera: pyralidae) in the lower rio grande valley of texas. | of approximately 18,200 ha planted to sugarcane in south texas, only approximately 80 ha (<0.5%) are treated with insecticides because this type of control is widely regarded as ineffective against stalkboring pyralids, the key pests of sugarcane. therefore, nonchemical control measures, such as resistant varieties and biological controls, must be evaluated to mitigate the losses caused by stalkborers. we performed laboratory and field evaluations on the use of beauveria bassiana (balsamo) vuill ... | 2000 | 14658512 |
| beauveria bassiana yeast phase on agar medium and its pathogenicity against diatraea saccharalis (lepidoptera: crambidae) and tetranychus urticae (acari: tetranychidae). | beauveria bassiana colonizes insect hosts initially through a yeast phase, which is common in some artificial liquid cultures, but not reported on artificial solid media. we describe a yeast-like phase for b. bassiana isolate 447 (atcc 20872) on macconkey agar and its virulence toward diatraea saccharalis and tetranychus urticae. the yeast-like cells of b. bassiana developed by budding from germinating conidia after 24-h incubation. cells were typically 5-10 microm and fungal colonies were initi ... | 2002 | 12445790 |
| electrophysiological studies and identification of possible sex pheromone components of brazilian populations of the sugarcane borer, diatraea saccharalis. | virgin female gland extracts of sugarcane moth diatraea saccharalis (fabricius) (lepidoptera: pyralidae), from three locations in brazil, have been analyzed. by gc-ms analysis and comparison of the chromatographic retention time of the components of the pheromone gland with those retention times of synthetic standards, we observed the presence of (z)-hexadec-11-enal (1), hexadecanal (2), (9e,11z)-hexadecadienal (4), (9z,11z)-hexadecadienal (5) and (9e,11e)-hexadecadienal (6), as minor components ... | 2002 | 12241007 |
| identification, syntheses, and characterization of the geometric isomers of 9,11-hexadecadienal from female pheromone glands of the sugar cane borer diatraea saccharalis. | chemical analysis of the pheromone glands of the sugar cane borer diatraea saccharalis has shown the presence of the four geometric isomers of 9,11-hexadecadienal (1-4), in addition to hexadecanal and (z)-hexadec-11-enal. we here report the syntheses and characterization of compounds 1-4. one starting material, 9-decen-1-ol, has been used to obtain all of them via divergent synthetic routes. | 2002 | 12088437 |
| conventional resistance of experimental maize lines to corn earworm (lepidoptera: noctuidae), fall armyworm (lepidoptera: noctuidae), southwestern corn borer (lepidoptera: crambidae), and sugarcane borer (lepidoptera: crambidae). | plant resistance is a useful component of integrated pest management for several insects that are economically damaging to maize, zea mays l. in this study, 15 experimental lines of maize derived from a backcross breeding program were evaluated for resistance to corn earworm, helicoverpa zea (boddie); fall armyworm, spodoptera frugiperda (j. e. smith); southwestern corn borer, diatraea grandiosella dyar; and sugarcane borer, diatraea saccharalis (f.). experimental line 100-r-3 was resistant in t ... | 2000 | 10902359 |
| genotyping isolates of the entomopathogenic fungus beauveria bassiana by rapd with fluorescent labels | random amplified polymorphic dna (rapd) with incorporation of fluorescent deoxynucleotides was used to examine the genetic diversity among beauveria bassiana isolates from argentina and brazil. high-resolution dna fingerprints were generated on line, during polyacrylamide gel electrophoresis of amplification products, by automated laser fluorescence analysis. each isolate displayed a distinct genotype. cluster analysis showed a high level of variability among these genotypes. no correlation with ... | 1998 | 9500948 |
| population dynamics of caterpillars on three cover crops before sowing cotton in mato grosso (brazil). | direct seeding mulch-based cropping systems under a preliminary cover crop such as millet are common in some areas of brazil. lepidopteran pests that damage cotton, soybean and maize crops can proliferate on cover crops, so preventive chemical treatments are necessary. very little data is available on these pests on cover crops. this paper presents the dynamics of spodoptera frugiperda, s. eridania, mocis latipes and diatraea saccharalis caterpillars monitored at primavera do leste, mato grosso ... | 2010 | 21539250 |
| f(2) screening for resistance to pyramided bacillus thuringiensis maize in louisiana and mississippi populations of diatraea saccharalis (lepidoptera: crambidae). | background: in the mid-southern region of the united states, sugarcane borer, diatraea saccharalis (f.), is a major target pest of transgenic maize expressing bacillus thuringiensis (bt) proteins. novel transgenic maize technologies containing two or more pyramided bt genes for controlling lepidopteran pests have recently become commercially available. insect resistance management (irm) is an important issue in the sustainable use of bt crop technologies. the objective of this study was to deter ... | 2011 | 21538799 |
| structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is an economically important pest of several major crops in north and south america. there is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of d. saccharalis, are needed. this article reports the complete mitochondrial genome sequen ... | 2011 | 20849253 |
| deleterious activity of natural products on postures of spodoptera frugiperda (lepidoptera: noctuidae) and diatraea saccharalis (lepidoptera: pyralidae). | the control of lepidoptera pests should be carried out before hatching of their caterpillars to avoid damage to the crops. the aim of this work was to assess the activity of neem (trade name: natuneem, producer: base fértil, chapadão do sul, brazil) and pyroligneous extracts (trade name: biopirol 7m, producer: biocarbo, itabirito, brazil) at 10 ml/l (1%) and 20 ml/l (2%) contents on egg masses of different ages of spodoptera frugiperda smith (lepidoptera: noctuidae) and of diatraea saccharalis f ... | 2010 | 20653245 |
| [effects of adult feeding on the reproduction and longevity of noctuidae, crambidae, tortricidae and elachistidae species]. | this research evaluates the effect of the adult diet on the reproduction of anticarsia gemmatalis hübner, heliothis virescens (fabr.), spodoptera frugiperda (j. e. smith) (noctuidae), diatraea saccharalis (fabr.) (crambidae), gymnandrosoma aurantianum lima (tortricidae) and stenoma catenifer walsingham (elachistidae). adults of all species were fed either water or a 10% honey solution. the egg viability for the 1st and 2nd egg masses, adult fecundity, longevity, number of mating and the ovigeny ... | 2010 | 20498952 |
| induction of a gloverin-like antimicrobial polypeptide in the sugarcane borer diatraea saccharalis challenged by septic injury. | diatraea saccharalis (fabricius, 1794) (lepidoptera: crambidae) is an important pest for brazilian sugarcane. in the present study, we detected two distinct spots in hemolymph from septic injured larvae (hds1 and hds2), which are separated by 2de gel electrophoresis. both spots were subjected to in-gel tryptic digestion and maldi-tof/tof analysis, which revealed the sequence vfgtlgsddsglfgk present in both hds1 and hds2. this sequence had homology and 80% identity with specific lepidoptera antim ... | 2010 | 20490430 |
| effects of entomopathogenic bacterium photorhabdus temperata infection on the digestive enzymes of diatraea saccharalis (lepidoptera: crambidae) larvae. | this study investigated the effects of photorhabdus temperata infection on the activities of digestive enzymes of the sugarcane stalk borer diatraea saccharalis. non-infected d. saccharalis larvae present a major alpha-amylase, several proteinases, three sucrose hydrolases and two alpha-glucosidases in their midgut. analysis of these hydrolases by electrophoresis and "in gel" assays showed that the activities of all enzymes decreased following infection, with an initial decline observed 12 h aft ... | 2008 | 18782059 |
| allele frequency of resistance to bacillus thuringiensis cry1ab corn in louisiana populations of sugarcane borer (lepidoptera: crambidae). | transgenic bacillus thuringiensis (bt) corn, zea mays l., has been widely used to manage a corn borer complex in the mid-southern region of the united states. the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), has become a dominant cornstalk boring species in some areas of this region, especially in louisiana. therefore, management of sugarcane borer resistance to bt corn is critical to ensure the long-term sustainability of bt corn for the region. this study screened 280 t ... | 2008 | 18459416 |
| effects of entomopathogenic bacterium photorhabdus temperata infection on the intestinal microbiota of the sugarcane stalk borer diatraea saccharalis (lepidoptera: crambidae). | photorhabdus temperata is an entomopathogenic bacterium that is associated with nematodes of the heterorhabditidae family in a symbiotic relationship. this study investigated the effects of p. temperata infection on the intestinal microbiota of the sugarcane stalk borer diatraea saccharalis. histopathology of the infection was also investigated using scanning electron microscopy. groups of 20 larvae were infected by injection of approximately 50 bacterial cells directly into the hemocoel. after ... | 2008 | 18406423 |
| effects of soybean proteinase inhibitors on development of the soil mite scheloribates praeincisus (acari: oribatida). | proteinase inhibitors (pi) are present in plant tissues, especially in seeds, and act as a defense mechanism against herbivores and pathogens. serine pi from soybean such as bowman-birk (bbpi) and kunitz have been used to enhance resistance of sugarcane varieties to the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae), the major pest of this crop. the use of these genetically-modified plants (gm) expressing pi requires knowledge of its sustainability and environmental sa ... | 2008 | 18357504 |
| comparison of three methods of parasitoid polydnavirus genomic dna isolation to facilitate polydnavirus genomic sequencing. | a major long-term goal of polydnavirus (pdv) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. as pdv viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be dissected to yield enough viral dna from the adult ovaries to carry out pdv genomic sequencing. this study compares three methods of pdv genomic dna isolation for the pdv of cotesia ... | 2008 | 18348210 |
| evaluation of transgenic bacillus thuringiensis corn hybrids against cry1ab-susceptible and -resistant sugarcane borer (lepidoptera: crambidae). | a louisiana strain of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), was selected for resistance to the crylab protein of bacillus thuringiensis (bt) by using an f2 screening procedure. survival of bt-resistant, -susceptible, and -heterozygous genotypes of sugarcane borer was evaluated on vegetative and reproductive stages of five non-bt and seven bt field corn, zea mays l., hybrids in a greenhouse study. larval survival was recorded 21 d after infestation of neonates o ... | 2007 | 18232406 |
| sugarcane borer (lepidoptera: crambidae) resistance to transgenic bacillus thuringiensis maize. | transgenic maize, zea mays l., expressing the bacillus thuringiensis (bt) crylab toxin has been planted to extensive areas across the united states and several other countries, but no resistance has been documented in field populations oflepidopteran target pests. this article describes the first report of resistance alleles to commercially available cry1ab bt maize in a louisiana population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). two hundred thirteen two-parent i ... | 2007 | 17370824 |
| production of 3-nitropropionic acid by endophytic fungus phomopsis longicolla isolated from trichilia elegans a. juss ssp. elegans and evaluation of biological activity. | the compound 3-nitropropionic acid is a potent neurotoxic agent in animals and well-known as a potent inhibitor of mycobacterium tuberculosis. in this research, we were able to extract this compound from the endophytic fungus, phomopsis longicolla (fj62759), isolated from trichilia elegans a. juss ssp. elegans. the aim of this study was the isolation of secondary metabolites produced by p. longicolla, the chemical identification of these compounds and evaluation of their antimicrobial and insect ... | 2013 | 23296917 |
| Identification and Structural Characterization of Novel Cyclotide with Activity against an Insect Pest of Sugar Cane. | Cyclotides are a family of plant-derived cyclic peptides comprising six conserved cysteine residues connected by three intermolecular disulfide bonds that form a knotted structure known as a cyclic cystine knot (CCK). This structural motif is responsible for the pronounced stability of cyclotides against chemical, thermal, or proteolytic degradation and has sparked growing interest in this family of peptides. Here, we isolated and characterized a novel cyclotide from Palicourea rigida (Rubiaceae ... | 2012 | 22074926 |
| defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified kunitz-type trypsin inhibitors and recruit two of them against papain. | multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. information about defense mechanisms of non-genomic model plant passion fruit (passiflora edulis sims) in response to predator attack is still limited. here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent kunitz-type trypsin inhibitors (ktis) in response to methyl jasmonate. seven inhibitors (20- ... | 2014 | 24849173 |
| seasonal infestations of two stem borers (lepidoptera: crambidae) in noncrop grasses of gulf coast rice agroecosystems. | infestations of two stem borers, eoreuma loftini (dyar) and diatraea saccharalis (f.) (lepidoptera: crambidae), were compared in noncrop grasses adjacent to rice (oryza sativa l.) fields. three farms in the texas rice gulf coast production area were surveyed every 6-8 wk between 2007 and 2009 using quadrat sampling along transects. although d. saccharalis densities were relatively low, e. loftini average densities ranged from 0.3 to 5.7 immatures per m(2) throughout the 2-yr period. early annual ... | 2011 | 22251716 |
| neem oil (azadirachta indica a. juss) affects the ultrastructure of the midgut muscle of ceraeochrysa claveri (navás, 1911) (neuroptera: chrysopidae). | cytomorphological changes, by means of ultrastructural analyses, have been used to determine the effects of the biopesticide neem oil on the muscle fibers of the midgut of the predator ceraeochrysa claveri. insects, throughout the larval period, were fed eggs of diatraea saccharalis treated with neem oil at a concentration of 0.5%, 1% or 2%. in the adult stage, the midgut was collected from female insects at two stages of adulthood (newly emerged and at the start of oviposition) and processed fo ... | 2017 | 27939524 |
| synergism and antagonism between bacillus thuringiensis vip3a and cry1 proteins in heliothis virescens, diatraea saccharalis and spodoptera frugiperda. | second generation bt crops (insect resistant crops carrying bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. some of the new combinations involve co-expression of cry and vip genes. because cry and vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two clas ... | 2014 | 25275646 |
| baseline susceptibility and monitoring of brazilian populations of spodoptera frugiperda (lepidoptera: noctuidae) and diatraea saccharalis (lepidoptera: crambidae) to vip3aa20 insecticidal protein. | the genetically modified maize expressing vip3aa20 insecticidal protein from bacillus thuringiensis berliner is abiotechnological option for the control of spodoptera frugiperda (j.e. smith) and diatraea saccharalis (f.) in brazil. to support an insect resistance management program, we conducted studies of baseline susceptibility and monitoring of brazilian populations of s. frugiperda and d. saccharalis to the vip3aa20 insecticidal protein. neonates were exposed to vip3aa20 applied on artificia ... | 2014 | 24772561 |
| managing the sugarcane borer, diatraea saccharalis, and corn earworm, helicoverpa zea, using bt corn and insecticide treatments. | the sugarcane borer, diatraea saccharalis (fabricius) (lepidoptera: crambidae) and the corn earworm, helicoverpa zea (boddie) (lepidoptera: noctuidae), are important pests of corn in brazil and have not been successfully managed, because of the difficulty of managing them with pesticides. the objective of this study was to evaluate the effect of bt corn mon810, transformed with a gene from bacillus thuringiensis berliner (bacillales: bacillaceae) insecticide seed treatment, and foliar insecticid ... | 2013 | 24735131 |
| fitness costs and stability of cry1ab resistance in sugarcane borer, diatraea saccharalis (f.). | the sugarcane borer, diatraea saccharalis (f.), is a major target species of transgenic corn expressing bacillus thuringiensis (bt) proteins in south america and the u.s. mid-south region. in this study, the fitness of seven insect genotypes of d. saccharalis were assayed on non-toxic diet, which included a cry1ab-susceptible strain (ss-2009), two cry1ab-resistant strains (rr-43a(bc), rr-l5b(bc)), and four f1 hybrids (f1-r43a(m)s(f), f1-r43a(f)s(m), f1-r5b(m)s(f), and f1-r5b(f)s(m)). the f1 hybr ... | 2014 | 24503242 |
| comparative binding of cry1ab and cry1f bacillus thuringiensis toxins to brush border membrane proteins from ostrinia nubilalis, ostrinia furnacalis and diatraea saccharalis (lepidoptera: crambidae) midgut tissue. | the european (ostrinia nubilalis hübner) and asian corn borers (ostrinia furnacalis guenée) are closely related and display similar sensitivity to cry1 toxins. in this study, we compared the binding patterns of cry1ab and cry1f toxins between both ostrinia spp., as well as the expression of putative cadherin- and aminopeptidase-n (apn)-like protein receptors. additionally, cdna sequences of these putative toxin receptors from both ostrinia species were compared. ligand blots for both species ind ... | 2013 | 23999243 |
| occurrence and larval movement of diatraea saccharalis (lepidoptera: crambidae) in seed mixes of non-bt and bt pyramid corn. | larval movement of target pest populations among bt and non-bt plants is a major concern in the use of a seed mixture refuge strategy for bt resistance management. in this study, occurrence and larval movement of the sugarcane borer, diatraea saccharalis (f.), were evaluated in four planting patterns of non-bt and bt plants containing genuity® smartstax(tm) traits in 2009-2011. the four planting patterns were: (1) a pure stand of 27 bt plants; (2) one non-bt plant in the center, surrounded by 26 ... | 2013 | 23456950 |
| extended monitoring of resistance to bacillus thuringiensis cry1ab maize in diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is a major target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. during 2007-2009, a total of 986 feral individuals of d. saccharalis were collected from maize fields in six locations of louisiana and mississippi and examined for resistance to cry1ab maize using f 1/f 2 screens. major resistance alleles to cry1ab maize in the populations sampled from non-bt maiz ... | 2014 | 22688686 |
| proteomic profile of hemolymph and detection of induced antimicrobial peptides in response to microbial challenge in diatraea saccharalis (lepidoptera: crambidae). | insects are organisms extremely well adapted to diverse habitats, primarily due to their innate immune system, which provides them with a range of cellular and humoral responses against microorganisms. lepidoptera hemolymph proteins involved in humoral responses are well known; however, there is a lack of knowledge about the sugarcane borer diatraea saccharalis. in this present work, the hemolymph proteins of this pest insect were studied by applying proteomic methodologies. two-dimensional elec ... | 2016 | 27012208 |
| sucrose hydrolases from the midgut of the sugarcane stalk borer diatraea saccharalis. | a beta-fructosidase (ec 3.2.1.26) was isolated from the midgut of larval sugar cane stalk borer diatraea saccharalis by mild-denaturing electrophoresis and further purified to near homogeneity by gel filtration. beta-fructosidase hydrolysed sucrose, raffinose and the fructosyl-trisaccharide isokestose, but it had no activity against maltose, melibiose and synthetic substrates for alpha-glucosidases. two other sucrose hydrolases, one resembling a alpha-glucosidase (ec 3.2.1.20) and the other one ... | 2004 | 15607512 |
| competition of bacillus thuringiensis cry1 toxins for midgut binding sites: a basis for the development and management of transgenic tropical maize resistant to several stemborers. | binding and competition of five bacillus thuringiensis toxins--cry1ab, cry1ac, cry1ba, cry1ca, and cry1ea--for midgut binding sites from three pests, spodoptera frugiperda, diatraea saccharalis, and diatraea grandiosella, were investigated as part of a strategy to develop tropical transgenic maize resistant to several stemborers. on s. frugiperda, cry1ab and cry1ac compete for the same binding site; cry1ba and cry1ca compete for a second binding site. cry1ea recognizes a third specific binding s ... | 2004 | 15297925 |
| possibly similar genetic basis of resistance to bacillus thuringiensis cry1ab protein in 3 resistant colonies of the sugarcane borer collected from louisiana, usa. | the sugarcane borer, diatraea saccharalis (f.), is a major maize borer pest and a target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. evolution of resistance in target pest populations is a great threat to the long-term efficacy of bt crops. in this study, we compared the genetic basis of resistance to cry1ab protein in 3 resistant colonies of sugarcane borer established from field populations in louisiana, ... | 2016 | 27860288 |
| reproductive isolation between two populations of diatraea saccharalis (f.) (lepidoptera: crambidae) from different host plant species and regions in argentina. | the sugarcane borer, diatraea saccharalis (f.), has a widespread distribution throughout the western hemisphere and is a pest of many crop plants including sugarcane, corn, sorghum and rice. the use of bacillus thuringiensis (bt) corn has been the primary tool for managing this species in corn fields. sugarcane borer control has been recently threatened by observations of susceptibility and/or resistance to certain varieties of bt corn and the protein used in many newer varieties. this has led t ... | 2016 | 27112423 |
| lepidoptera (crambidae, noctuidae, and pyralidae) injury to corn containing single and pyramided bt traits, and blended or block refuge, in the southern united states. | fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae); corn earworm, helicoverpa zea boddie (lepidoptera: noctuidae); southwestern corn borer, diatraea grandiosella dyar (lepidoptera: crambidae); sugarcane borer, diatraea saccharalis f. (lepidoptera: crambidae); and lesser cornstalk borer, elasmopalpus lignosellus zeller (lepidoptera: pyralidae), are lepidopteran pests of corn, zea mays l., in the southern united states. blended refuge for transgenic plants expressing the i ... | 2015 | 26470116 |
| identification, inheritance, and fitness costs of cry2ab2 resistance in a field-derived population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is one of the major target pests of transgenic maize, zea mays, expressing bacillus thuringiensis (bt) proteins in south america and mid-southern region of the u.s. the mon89034 maize expresses cry2ab2 and cry1a.105 bt proteins and it was developed to provide better control of key lepidopteran pests of maize including d. saccharalis. the objectives of this study were to select and characterize the resistance of d. saccharalis to cry2ab2 using a non ... | 2015 | 26205174 |
| binding analysis of bacillus thuringiensis cry1 proteins in the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | sugarcane borer (diatraea saccharalis, f.) is an important corn pest in south america and united states. the aim of the present study was to analyze the susceptibility and binding interactions of three cry1a proteins and cry1fa in a brazilian d. saccharalis population. the results showed that cry1ab was the most active, followed by cry1ac, cry1fa and cry1aa. all cry1-biotinylated proteins tested bound specifically to the d. saccharalis brush border membrane vesicles (bbmv). heterologous competit ... | 2015 | 25736726 |
| modeling evolution of resistance of sugarcane borer (lepidoptera: crambidae) to transgenic bt corn. | diatraea saccharalis (f.) (lepidoptera: crambidae) is a target pest of transgenic corn expressing bacillus thuringiensis (bt) protein, and the first evidence of resistance by d. saccharalis to cry1ab corn was detected in a field population in northeast louisiana in 2004. we used a model of population dynamics and genetics of d. saccharalis to 1) study the effect of interfield dispersal, the first date that larvae enter diapause for overwintering, toxin mortality, the proportion of non-bt corn in ... | 2014 | 24914780 |
| side effects of neem oil on the midgut endocrine cells of the green lacewing ceraeochrysa claveri (navás) (neuroptera: chrysopidae). | we described the ultrastructure of ceraeochrysa claveri (navás) midgut endocrine cells in larva, pupa, and adult, and evaluated the side effects of ingested neem oil, a botanical insecticide obtained from the seeds of the neem tree (azadirachta indica), on these cells. during the larval period, c. claveri were fed (ad libitum) diatraea saccharalis (f.) eggs treated with neem oil at concentrations of 0.5%, 1%, or 2%. transmission electron microscopy showed that two subtypes of endocrine cells, na ... | 2014 | 27193522 |
| effects of neem oil (azadirachta indica a. juss) on midgut cells of predatory larvae ceraeochrysa claveri (navás, 1911) (neuroptera: chrysopidae). | the effects of ingested neem oil, a botanical insecticide obtained from the seeds of the neem tree, azadirachta indica, on the midgut cells of predatory larvae ceraeochrysa claveri were analyzed. c. claveri were fed on eggs of diatraea saccharalis treated with neem oil at a concentration of 0.5%, 1% and 2% during throughout the larval period. light and electron microscopy showed severe damages in columnar cells, which had many cytoplasmic protrusions, clustering and ruptured of the microvilli, s ... | 2013 | 22739123 |
| control of diatraea saccharalis by the endophytic pantoea agglomerans 33.1 expressing cry1ac7. | despite the fact that bacillus thuringiensis (bt) is found in more than 90 % of the products used against insects, it has some difficulty reaching the internal regions where the larvae feed. to solve this problem, many genetically modified microorganisms that colonize the same pests have been developed. thus, the endophytic bacterium pantoea agglomerans (33.1), which has been recently described as a promising sugarcane growth promoter, was genetically modified with the pjtt vector (which carries ... | 2014 | 24531524 |
| genetic diversity analysis with rapd linked to sex identification in the sugar cane borer diatraea saccharalis. | diatraea saccharalis is an insect that causes considerable losses in the sugar cane crop. our aim was to contribute to the knowledge of the biology of d. saccharalis, with the report of dna fragments involved in the differentiation between the male and female of this species using the rapd sex molecular marker gyakuu-13, which is specific for the w chromosome of bombyx mori. another point evaluated in this study was the genetic diversity of a d. saccharalis population maintained by inbreeding in ... | 2010 | 21128215 |
| bowman-birk proteinase inhibitor from clitoria fairchildiana seeds: isolation, biochemical properties and insecticidal potential. | herein described is the biochemical characterisation, including in vitro and in vivo assays, for a proteinase inhibitor purified from clitoria fairchildiana seeds (cfpi). purification was performed by hydrophobic interaction and gel filtration chromatography. kinetic studies of the purified inhibitor showed a competitive-type inhibitory activity against bovine trypsin and chymotrypsin, with an inhibition stoichiometry of 1:1 for both enzymes. the inhibition constants against trypsin and chymotry ... | 2015 | 26330217 |
| molecular cloning and insecticidal effect of inga laurina trypsin inhibitor on diatraea saccharalis and heliothis virescens. | native inga laurina (fabaceae) trypsin inhibitor (ilti) was tested for anti-insect activity against diatraea saccharalis and heliothis virescens larvae. the addition of 0.1% ilti to the diet of d. saccharalis did not alter larval survival but decreased larval weight by 51%. the h. virescens larvae that were fed a diet containing 0.5% ilti showed an 84% decrease in weight. ilti was not digested by the midgut proteinases of either species of larvae. the trypsin levels were reduced by 55.3% in the ... | 2012 | 22885277 |
| evaluation of corn hybrids expressing cry1f, cry1a.105, cry2ab2, cry34ab1/cry35ab1, and cry3bb1 against southern united states insect pests. | studies were conducted across the southern united states to characterize the efficacy of multiple bacillus thuringiensis (bt) events in a field corn, zea mays l., hybrid for control of common lepidopteran and coleopteran pests. cry1f protein in event tc1507 and cry1a.105 + cry2ab2 proteins in event mon 89034 were evaluated against pests infesting corn on above-ground plant tissue including foliage, stalks, and ears. cry34ab1/cry35ab1 proteins in event das-59122-7 and cry3bb1 in event mon 88017 w ... | 2012 | 23156183 |
| bacillus thuringiensis monogenic strains: screening and interactions with insecticides used against rice pests. | the screening of bacillus thuringiensis (bt) cry proteins with high potential to control insect pests has been the goal of numerous research groups. in this study, we evaluated six monogenic bt strains (bt dendrolimus hd-37, bt kurstaki hd-1, bt kurstaki hd-73, bt thuringiensis 4412, bt kurstaki nrd-12 and bt entomocidus 60.5, which codify the cry1aa, cry1ab, cry1ac, cry1ba, cry1c, cry2a genes respectively) as potential insecticides for the most important insect pests of irrigated rice: spodopte ... | 2012 | 24031872 |
| parasitism by the endoparasitoid, cotesia flavipes induces cellular immunosuppression and enhances susceptibility of the sugar cane borer, diatraea saccharalis to bacillus thuringiensis. | cotesia flavipes cameron (hymenoptera: braconidae), is a gregarious larval endoparasitoid of the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: crambidae). the aim of this research was to analyze cellular immunosuppression of d. saccharalis parasitized by c. flavipes in terms of encapsulation, melanization, and hemocyte nodule formation. the encapsulation assay was done 1 and 6 days after parasitoid oviposition. in addition, the susceptibility of parasitized and nonparasitzed larv ... | 2011 | 22225507 |
| a betabaculovirus encoding a gp64 homolog. | a betabaculovirus (disagv) was isolated from diatraea saccharalis (lepidoptera: crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in brazil. | 2016 | 26847652 |
| differential expression of the pr1a gene in metarhizium anisopliae and metarhizium acridum across different culture conditions and during pathogenesis. | the entomopathogenic fungi of the genus metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. the identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. in addition, the pr1a gene has been used for the genetic improvemen ... | 2015 | 25983629 |
| short and long-term antinutritional effect of the trypsin inhibitor apti for biological control of sugarcane borer. | plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. in this work, we tested the anti-insect activity of adenanthera pavonina trypsin inhibitor (apti) against diatraea saccharalis larvae, a major insect pest in sugarcane. the addition of 0.1% apti in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. apti was not digested after 60h incubation with ... | 2014 | 24355140 |
| evaluation of in vitro and in vivo effects of semipurified proteinase inhibitors from theobroma seeds on midgut protease activity of lepidopteran pest insects. | we have characterized in vitro and in vivo effects of trypsin inhibitors from theobroma seeds on the activity of trypsin- and chymotrypsin-like proteins from lepidopteran pest insects. the action of semipurified trypsin inhibitors from theobroma was evaluated by the inhibition of bovine trypsin and chymotrypsin activities determined by the hydrolysis of n-benzoyl-dl-arginine-p-nitroanilide (bapa) and n-succinyl-ala-ala-pho-phe p-nitroanilide (s-(ala)2prophe-pna). proteinase inhibitor activities ... | 2012 | 22806759 |
| cytotoxic effects of neem oil in the midgut of the predator ceraeochrysa claveri. | studies of morphological and ultrastructural alterations in target organs have been useful for evaluating the sublethal effects of biopesticides regarded as safe for non-target organisms in ecotoxicological analyses. one of the most widely used biopesticides is neem oil, and its safety and compatibility with natural enemies have been further clarified through bioassays performed to analyze the effects of indirect exposure by the intake of poisoned prey. thus, this study examined the cellular res ... | 2016 | 26520254 |
| critical electrolyte concentration of silk gland chromatin of the sugarcane borer diatraea saccharalis, induced using agrochemicals. | the sugarcane borer diatraea saccharalis is widely known as the main pest of sugarcane crop, causing increased damage to the entire fields. measures to control this pest involve the use of chemicals and biological control with cotesia flavipes wasps. in this study, we evaluated the insecticides fipronil (frontline; 0.0025%), malathion (malatol bio carb; 0.4%), cipermetrina (galgotrin; 10%), and neem oil (natuneem; 100%) and the herbicide nicosulfuron (sanson 40 sc; 100%) in the posterior region ... | 2014 | 25299111 |
| efficacy of insect growth regulators and diamide insecticides for control of stem borers (lepidoptera: crambidae) in sugarcane. | field experiments assessed control of the stem borers, diatraea saccharalis (f.) and eoreuma loftini (dyar), in sugarcane (saccharum spp.) with selected insect growth regulators and diamide insecticides. tebufenozide, novaluron, chlorantraniliprole, and flubendiamide were evaluated for d. saccharalis control in four field experiments in louisiana between 2009 and 2014. aerial application of the same insecticides was evaluated for control of e. loftini in commercial sugarcane fields in southern t ... | 2017 | 28334153 |
| host-plant associated genetic divergence of two diatraea spp. (lepidoptera: crambidae) stemborers on novel crop plants. | diatraea lineolata and diatraea saccharalis (lepidoptera: crambidae) are moths with stemboring larvae that feed and develop on economically important grasses. this study investigated whether these moths have diverged from a native host plant, corn, onto introduced crop plants including sorghum, sugarcane, and rice. diatraea larvae were collected from these four host plants throughout the year in el salvador and were reared on artificial diet until moths or parasitoids emerged. adult moths were s ... | 2016 | 28031813 |
| sugarcane serine peptidase inhibitors, serine peptidases, and clp protease system subunits associated with sugarcane borer (diatraea saccharalis) herbivory and wounding. | sugarcane's (saccharum spp.) response to diatraea saccharalis (f.) (lepidoptera: (crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane expressed sequence tags (ests) encoding serine peptidase inhibitors, serine peptidases. and clp protease system subunits. our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. among the upregulated genes, nine were similar to serine peptidase inhibitors and four were ... | 2016 | 27598134 |
| restriction site associated dna (rad) for de novo sequencing and marker discovery in sugarcane borer, diatraea saccharalis fab. (lepidoptera: crambidae). | we present the development of a genomic library using radseq (restriction site associated dna sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer diatraea saccharalis, an important south american insect pest. a radtag protocol combined with illumina paired-end sequencing allowed de novo discovery of 12 811 snps and a high-quality assembly of 122.8m paired-end reads from six individuals, representing 40 gb of sequencing data. approximately ... | 2016 | 27571734 |
| transgenic sugarcane with a cry1ac gene exhibited better phenotypic traits and enhanced resistance against sugarcane borer. | we developed sugarcane plants with improved resistance to the sugarcane borer, diatraea saccharalis (f). an expression vector pgcry1ac0229, harboring the cry1ac gene and the selectable marker gene, bar, was constructed. this construct was introduced into the sugarcane cultivar fn15 by particle bombardment. transformed plantlets were identified after selection with phosphinothricin (ppt) and basta. plantlets were then screened by pcr based on the presence of cry1ac and 14 cry1ac positive plantlet ... | 2016 | 27093437 |
| agricultural case studies of classification accuracy, spectral resolution, and model over-fitting. | this paper describes the relationship between spectral resolution and classification accuracy in analyses of hyperspectral imaging data acquired from crop leaves. the main scope is to discuss and reduce the risk of model over-fitting. over-fitting of a classification model occurs when too many and/or irrelevant model terms are included (i.e., a large number of spectral bands), and it may lead to low robustness/repeatability when the classification model is applied to independent validation data. ... | 2013 | 24160886 |
| comparative analysis of expression profiling of the trypsin and chymotrypsin genes from lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors. | peptidase inhibitors (pis) are essential proteins involved in plant resistance to herbivorous insects, yet many insect species are able to escape the negative effects of these molecules. we compared the effects of acute and chronic ingestion of soybean peptidase inhibitors (spis) on spodoptera frugiperda and diatraea saccharalis, two lepidoptera species with different sensitivities to spi ingestion. we analyzed the trypsin and chymotrypsin gene expression profiles in both species. acute exposure ... | 2017 | 26944308 |
| microsatellite loci and genetic structure of artificial populations of cotesia flavipes (hymenoptera, braconidae). | cotesia flavipes (cameron) is a parasitoid wasp used in the biological control of the sugarcane borer (diatraea saccharalis) (fabr., 1794). studies on the genetic diversity of c. flavipes are hampered by the lack of highly polymorphic molecular markers. in this report, a set of 11 microsatellite loci were developed from an enriched library of c. flavipes. four microsatellite loci were polymorphic and were screened in 212 c. flavipes individuals (183 females and 29 males) that were randomly sampl ... | 2016 | 27886334 |
| microarray analysis of global gene regulation in the cry1ab-resistant and cry1ab-susceptible strains of diatraea saccharalis. | extensive adoption of transgenic bt corn in recent years for stalk borer control has increased risk of resistance evolution in the target pest populations. a bt-resistant strain of the sugarcane borer, diatraea saccharalis, was approximately 100-fold more tolerant to cry1ab toxin than the susceptible counterpart. to gain a better understanding of the molecular mechanisms of bt resistance, the cry1ab-susceptible (cry1ab-ss) and cry1ab-resistant (cry1ab-rr) strains of d. saccharalis were subjected ... | 2012 | 22228544 |
| exploitation of mitochondrial nad6 as a complementary marker for studying population variability in lepidoptera. | the applicability of mitochondrial nad6 sequences to studies of dna and population variability in lepidoptera was tested in four species of economically important moths and one of wild butterflies. the genetic information so obtained was compared to that of cox1 sequences for two species of lepidoptera. nad6 primers appropriately amplified all the tested dna targets, the generated data proving to be as informative and suitable in recovering population structures as that of cox1. the proposal is ... | 2011 | 22215980 |
| quality of the exotic parasitoid cotesia flavipes (hymenoptera: braconidae) does not show deleterious effects after inbreeding for 10 generations. | although the parasitoid cotesia flavipes (cameron) has proven effective in controlling sugarcane borer diatraea saccharalis (fabricius) for many years, concern has arisen over the quality of individuals produced at large scales. the parasitoid has been reared in laboratories in brazil for more than 40 years, with no new introductions of new populations during that period. since the quality of the parasitoids was not verified at the time of the species' introduction in brazil, we do not know if t ... | 2016 | 27509087 |
| a two-agent model applied to the biological control of the sugarcane borer (diatraea saccharalis) by the egg parasitoid trichogramma galloi and the larvae parasitoid cotesia flavipes. | the paper is aimed at a methodological development in biological pest control. the considered one pest two-agent system is modelled as a verticum-type system. originally, linear verticum-type systems were introduced by one of the authors for modelling certain industrial systems. these systems are hierarchically composed of linear subsystems such that a part of the state variables of each subsystem affect the dynamics of the next subsystem. recently, verticum-type system models have been applied ... | 2016 | 26911807 |
| development of a new container for storage and release of the parasitoid cotesia flavipes (hymenoptera: braconidae). | in brazil, the parasitoid cotesia flavipes (cameron) has been used as a biological control agent against diatraea saccharalis (f.) since 1976, because of its effectiveness in reducing populations of this pest. three million plastic cups are used annually to release c. flavipes, which remain in the environment. this study aimed to develop fully biodegradable packaging, so that releases occur without damaging the environment. the biological characteristics of c. flavipes were studied for five gene ... | 2015 | 26470218 |
| impacts of nosema sp. (microsporidia: nosematidae) on the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | in brazil, the sugarcane borer, diatraea saccharalis (fabricius, 1794) (lepidoptera: crambidae), is controlled with massive releases of the hymenopteran parasitoid cotesia flavipes cam. (hymenoptera: braconidae); over 3 million hectares of sugarcane are treated annually with 18 billion parasitoids. in order to meet this demand, parasitoids are produced in d. saccharalis under laboratory conditions where a nosema sp. is reported to be an important problem in mass rearing of the host. the goals fo ... | 2015 | 25981134 |
| the parasitoid, cotesia flavipes (cameron) (hymenoptera: braconidae), influences food consumption and utilization by larval diatraea saccharalis (f.) (lepidoptera: crambidae). | parasitoids exploit host insects for food and other resources; they alter host development and physiology to optimize conditions to favor parasitoid development. parasitoids influence their hosts by injecting eggs, along with a variety of substances, including venoms, polydnaviruses, ovarian fluids, and other maternal factors, into hosts. these factors induce profound changes in hosts, such as behavior, metabolism, endocrine events, and immune defense. because endoparasitoids develop and consume ... | 2014 | 25042594 |
| feasibility of using an alternative larval host and host plants to establish cotesia flavipes (hymenoptera: braconidae) in the temperate louisiana sugarcane ecosystem. | attempts to establish cotesia flavipes cameron (hymenoptera: braconidae) in louisiana sugarcane fields to control the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae) have been unsuccessful. experiments were conducted to investigate the feasibility of using an alternative larval host and host plants to overcome barriers preventing establishment. in addition, we evaluated c. flavipes' ability to search for d. saccharalis in sugarcane without above-ground internodes. diatraea ev ... | 2012 | 22506999 |
| inorganic elements in the fat bodies of diatraea saccharalis (lepidoptera: crambidae) larvae parasitized by cotesia flavipes (hymenoptera: braconidae). | koinobiont parasitoids use several strategies to regulate the host's physiological processes during parasitism. although many aspects of host-parasitoid interactions have been explored, studies that attempted to assess the effects of parasitism on the availability of inorganic elements in the host are virtually nonexistent. therefore, we aimed to evaluate the effects of parasitism on the concentrations of inorganic elements in the fat bodies of larvae of diatraea saccharalis (lepidoptera: crambi ... | 2010 | 20388551 |
| mitochondria in the midgut epithelial cells of sugarcane borer parasitized by cotesia flavipes (cameron, 1891). | the sugarcane borer diatraea saccharalis (lepidoptera: crambidae) has been controlled by cotesia flavipes (hymenoptera: braconidae); however, very little is known about the effect of the parasitism in the host organs, including the midgut. this work aims to verify mitochondrial alteration in the different midgut epithelial cells of d. saccharalis parasitized by c. flavipes. midgut fragments (anterior and posterior region) of both non-parasitized and parasitized larvae were processed for transmis ... | 2010 | 20231974 |
| spherites in the midgut epithelial cells of the sugarcane borer parasitized by cotesia flavipes. | diatraea saccharalis, the main pest of sugarcane, has been controlled by cotesia flavipes. very little is known about the effect of parasitism on the host organs, including the midgut. the lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. spherites have been described in columnar and regenerative cells of several lepidoptera species, and presented a lot of functional meaning. we identified spherites in the midgut epithelial cells of non-parasitized ... | 2008 | 18669324 |
| morphometry of the midgut epithelium of diatraea saccharalis fabricius, 1794 (lepidoptera) parasitized by cotesia flavipes cameron, 1891 (hymenoptera). | the morphometric study of the midgut in diatraea saccharalis (lepidoptera) larvae parasitized by the cotesia flavipes (hymenoptera) showed that there was significant increase in the columnar, goblet and regenerative cells and their nuclei; the midgut lumen diameter and the epithelial height were also increased in the parasitized larvae. the multivariate analysis showed that parasitism affected the columnar cell only in the posterior region, and the goblet cells along the midgut length (anterior ... | 2006 | 16793057 |
| biological aspects of eriopis connexa (germar) (coleoptera: coccinellidae) fed on different insect pests of maize (zea mays l.) and sorghum [sorghum bicolor l. (moench.)]. | eriopis connexa (germar) (coleoptera: coccinellidae) occurs in several countries of south america and its mass rearing is important for biological control programmes. this work evaluated biological aspects of e. connexa larva fed on eggs of anagasta kuehniella (zeller) (lepidoptera: pyralidae) and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae) frozen for one day, fresh eggs of diatraea saccharalis (fabricius) (lepidoptera: pyralidae), s. frugiperda newly-hatched caterpillars, nymph ... | 2013 | 23917572 |
| effect of silicon soil amendment on performance of sugarcane borer, diatraea saccharalis (lepidoptera: crambidae) on rice. | the sugarcane borer, diatraea saccharalis (f.), is a pest of graminaceous crops in the southern usa, including sugarcane, maize, and rice. this study was conducted to investigate the effect of silicon (si) soil amendments on performance of sugarcane borer, d. saccharalis, on two rice cultivars, cocodrie and xl723. there was a significant increase in the si content of rice plants supplemented with calcium silicate as compared to non-treated plants. soil si amendment led to lower relative growth r ... | 2013 | 23830057 |
| development and characterization of microsatellite loci for genetic studies of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | we present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, diatraea saccharalis (fabricius). we isolated 16 microsatellite loci through an enriched genomic library protocol. after characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the polymorphism information content (ranging from 0.292 to 0.771; 0.535 on average). these markers can be used in further studies to ... | 2013 | 23765969 |
| susceptibility of cry1ab maize-resistant and -susceptible strains of sugarcane borer (lepidoptera: crambidae) to four individual cry proteins. | sugarcane borer, diatraea saccharalis (f.), is a major target of bt maize in south america and many areas of the us mid-south region. six laboratory strains of d. saccharalis were established from six single-pair f(2) families possessing major resistance alleles to cry1ab maize hybrids. susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated bt proteins, cry1ab, cry1aa, cry1ac and cry1f. bt susceptibility of the six strains was compared with t ... | 2013 | 23270874 |
| oviposition behavior of diatraea saccharalis (lepidoptera: crambidae) on different rice cultivars in louisiana. | the sugarcane borer, diatraea saccharalis (f.), is a pest of a variety of graminaceous crops in the southern united states, including sugarcane, maize, and rice in louisiana. this study examined several aspects of d. saccharalis oviposition behavior on rice (oryza sativa). the vertical distribution of egg masses on four phenological stages of rice in the field showed that d. saccharalis prefers to oviposit on the uppermost portions of rice plants, regardless of plant stage. in greenhouse choice ... | 2012 | 22732615 |