| a new risk of occupational disease: allergic asthma and rhinoconjunctivitis in persons working with beneficial arthropods. preliminary data. | for some years, biological pest control has been re-evaluated as an alternative to the indiscriminate use of pesticides. the number of "bio-factories" in europe has been constantly increasing and today more than 100 technicians work in mass rearing of arthropods. seven cases of asthma and allergic rhinitis are reported in 13 workers engaged in the production of beneficial arthropods. the subjects suffered from attacks of asthma and rhinitis when they came into contact with eggs and scales or was ... | 1994 | 8175182 |
| molecular differentiation of sex chromosomes probed by comparative genomic hybridization. | comparative genomic hybridization (cgh) was used to identify and probe sex chromosomes in several xy and wz systems. chromosomes were hybridized simultaneously with fluorx-labelled dna of females and cy3-labelled dna of males in the presence of an excess of cot-1 dna or unlabelled dna of the homogametic sex. cgh visualized the molecular differentiation of the x and y in the house mouse, mus musculus, and in drosophila melanogaster: while autosomes were stained equally by both probes, the x and y ... | 1999 | 10398846 |
| digestion of insect chromatin with micrococcal nuclease, dnase i and dnase i combined with single-strand specific nuclease s1. | the chromatin of the lepidopteran ephestia kuehniella was digested by micrococcal nuclease, dnase i and s1-nuclease combined with dnase i pretreatment. the resulting dna fragments were analyzed by gel electrophoresis and compared with the dna fragments of rat liver nuclei obtained by the same process. extensive homology was revealed between insect and mammalian chromatin structure. the combined dnase i- s1-nuclease digestion yields double-stranded dna fragments of lengths from 30 to 110 base-pai ... | 1977 | 909768 |
| comparison of microfilament patterns in nurse cells of different insects with polytrophic and telotrophic ovarioles. | the localization of f-actin (microfilaments) in the nurse cells of ovarian follicles has been studied in 12 different insect species by fluorescence microscopy after specifically staining f-actin with rhodamine-conjugated phalloidin. in the analysed species with polytrophic ovaries (apis mellifica, pimpla turionellae, bradysia tritici, ephestia kuehniella, protophormia terraenovae) a dense f-actin network was found to be associated with the nurse cell membranes. only in protophormia were microfi ... | 1986 | 3734686 |
| transinfection of wolbachia in the mediterranean flour moth, ephestia kuehniella, by embryonic microinjection. | wolbachia are maternally transmitted intracellular bacteria found in many arthropod species. they cause a reproductive incompatibility called cytoplasmic incompatibility (ci) in several hosts, including the mediterranean flour moth, ephestia kuehniella. two strains of e. kuehniella, one from yokohama city and the other from tsuchiura city, express different levels of ci: the yokohama strain expresses ci at a higher level than the tsuchiura strain. in order to determine whether the difference of ... | 2000 | 11012714 |
| the effect of gamma and microwave irradiation of wheat grain on development parameters of some stored grain pests. | wheat grain was treated with different doses of gamma irradiation or different times of microwaves. gamma rays (0.05-10 kgy) caused statistically significant differences in soluble protein contents and kernel hardness. microwave treatment (15-180 s) resulted in some statistically significant changes in moisture and soluble protein contents. mortality, intensity of feeding, fertility of adults, progeny number and development time of larvae of the granary weevil (sitophilus granarius l.) on treate ... | 2000 | 11190835 |
| phylogeny of the arthropod endosymbiont wolbachia based on the wsp gene. | bacteria of the genus wolbachia (rickettsiae) are widespread in arthropods and can induce cytoplasmic incompatibility (ci), thelytoky (t) or feminization (f) in their host. recent research on the wsp gene of mainly ci inducing wolbachia has shown that this gene evolves at a much faster rate than previously sequenced genes such as 16s or ftsz. as a result this gene appears to be very useful in subdividing the wolbachia and twelve groups have been distinguished to date. here we extend the wolbachi ... | 1999 | 10469257 |
| [sensitization and allergic pathology in a group of workers employed in insect breeding for biological pest control]. | on the basis of the results of a previous study and immuno-allergological tests, a group of workers employed in breeding insects for biological pest control were studied to ascertain whether any new sensitizations to arthropods recently included in the work cycle occurred and whether any new allergic diseases were observed. the results confirmed a high sensitization to ephestia kuehniella and to orius, the latter probably due to contamination; sensitization to tetranicus urticae and to planococc ... | 1997 | 9379990 |
| interspecific transfer of wolbachia between two lepidopteran insects expressing cytoplasmic incompatibility: a wolbachia variant naturally infecting cadra cautella causes male killing in ephestia kuehniella. | wolbachia is known as the causative agent of various reproductive alterations in arthropods. the almond moth cadra cautella is doubly infected with a- and b-group wolbachia and expresses complete cytoplasmic incompatibility (ci). the mediterranean flour moth ephestia kuehniella carries a-group wolbachia and expresses partial ci. in the present study, the wolbachia in c. cautella was transferred to e. kuehniella from which the original wolbachia had been removed. we obtained transfected lines of ... | 2002 | 12454075 |
| ttagg telomeric repeats in chromosomes of some insects and other arthropods. | we studied the occurrence of the ttagg telomere repeats by fluorescence in-situ hybridization (fish) and southern hybridization in ten insect species and two other arthropods. (ttagg)n-containing telomeres were found in three lepidoptera species, the silkworm bombyx mori (in which the telomeric sequence was recently discovered), the flour moth ephestia kuehniella, and the wax moth galleria mellonella, in one species of hymenoptera, the honey bee apis mellifera, in one species of coleoptera, the ... | 1999 | 10560968 |
| evaluation of diets for the development and reproduction of franklinothrips orizabensis (thysanoptera: aeolothripidae). | the suitability of ten diets for the development and reproduction of franklinothrips orizabensis johansen, the key natural enemy of scirtothrips perseae nakahara, a pest of california grown avocados, was determined in the laboratory. the experimental diets evaluated were: (i) irradiated ephestia kuehniella zeller eggs; (ii) irradiated e. kuehniella eggs and avocado pollen; (iii) tetranychus pacificus mcgregor eggs; (iv) t. pacificus eggs and avocado pollen; (v) irradiated e. kuehniella eggs and ... | 2001 | 11587623 |
| regulation of wolbachia density in the mediterranean flour moth, ephestia kuehniella, and the almond moth, cadra cautella. | the mediterranean flour moth, ephestia kuehniella, is infected with a-group wolbachia (wkue), and the almond moth, cadra cautella, is doubly infected with a- and b-group wolbachia, which are designated as wcaua and wcaub, respectively. in both insects, the wolbachia populations increased greatly during embryonic and larval stages. the wolbachia population doubled every 3.6 days on average in e. kuehniella larvae, whereas those of wcaua and wcaub doubled every 2.1 days in c. cautella larvae. the ... | 2003 | 12655178 |
| transfection of wolbachia in lepidoptera: the feminizer of the adzuki bean borer ostrinia scapulalis causes male killing in the mediterranean flour moth ephestia kuehniella. | two species of lepidoptera, ostrinia scapulalis and ephestia kuehniella, harbour wolbachia, which are maternally transmitted intracellular bacteria that often cause reproductive abnormalities in arthropods. while the infection in o. scapulalis causes conversion of genetic males into functional females (feminization), that in e. kuehniella induces cytoplasmic incompatibility. in the present study, we investigated the relative importance of host and wolbachia factors in the differential expression ... | 2001 | 11345332 |
| a trypsin inhibitor from peltophorum dubium seeds active against pest proteases and its effect on the survival of anagasta kuehniella (lepidoptera: pyralidae). | a novel trypsin inhibitor was purified from the seeds of peltophorum dubium (spreng.). sds-page under reducing conditions showed that the inhibitor consisted of a single polypeptide chain (ca. 20 kda). the dissociation constants of 4 x 10(-10) and 1.6 x 10(-10) m were obtained with bovine and porcine trypsin, respectively. this constant was lower (2.6 x 10(-7) m) for chymotrypsin. the inhibitory activity was stable over a wide range of temperature and ph and in the presence of dtt. the n-termina ... | 2003 | 12726993 |
| the development of the endoparasitoid venturia canescens in bt-tolerant, immune induced larvae of the flour moth ephestia kuehniella. | we examined the survival and development of the endoparasitoid venturia canescens in a bt-tolerant laboratory strain of the flour moth ephestia kuehniella, in which bt-tolerance has been shown to be associated with an inducible elevation of the insect's immune response. the results indicate the elevated immune status associated with bt-tolerance does not confer cross-protection against parasitism by v. canescens. no significant difference was observed in the rate of emergent wasps from parasitis ... | 2004 | 15579322 |
| infection density of wolbachia and level of cytoplasmic incompatibility in the mediterranean flour moth, ephestia kuehniella. | wolbachia, a causative agent of various reproductive changes in arthropods, induces cytoplasmic incompatibility (ci) in the mediterranean flour moth, ephestia kuehniella. two strains of e. kuehniella, yokohama and tsuchiura, harbor closely related wolbachia, but the yokohama strain expresses stronger ci than the tsuchiura strain. a transinfected e. kuehniella strain that harbors the wolbachia derived from the almond moth cadra cautella, expresses weak ci at a similar level to the tsuchiura strai ... | 2003 | 13678706 |
| induction and transmission of bacillus thuringiensis tolerance in the flour moth ephestia kuehniella. | the use of bacillus thuringiensis (bt) endotoxins to control insect vectors of human diseases and agricultural pests is threatened by the possible evolution of resistance in major pest species. in addition to high levels of resistance produced by receptor insensitivity (5, 16, 17), several cases of tolerance to low to medium levels of toxin have been reported in laboratory colonies of lepidopteran species (3, 18). because the molecular basis of some of these cases of tolerance to the toxin are n ... | 2004 | 14978282 |
| wolbachia variant that induces two distinct reproductive phenotypes in different hosts. | wolbachia is an intracellular endosymbiont that induces a variety of reproductive alterations in diverse arthropods. the almond moth, cadra cautella, is double infected with two wolbachia variants, wcaua and wcaub, and expresses complete cytoplasmic incompatibility (ci). the individual contribution of wcaua and wcaub to the expression of ci are unclear, however, because the two variants have not been separated in this host. the effect of wcaua is of particular interest because it induces male ki ... | 2005 | 16106260 |
| trypsin inhibitor from poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases. | plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves of being attacked by insects. in this work, a novel trypsin inhibitor (ppti) was purified from the seeds of the native brazilian tree poecilanthe parviflora (benth) (papilioinodeae, leguminosae) by gel filtration chromatography on a sephadex g-100 followed by superdex g75 chromatography (fplc), sepharose 4b-trypsin column, and fractionated by reversed-phase hplc on a c-18 column. sds-pag ... | 2004 | 15328890 |
| nymphal development and feeding preference of podisus maculiventris (heteroptera: pentatomidae) on eggs of ephestia kuehniella (lepidoptera: pyralidae) parasitised or not by trichogramma brassicae (hymenoptera: trichogrammatidae). | predation by podisus maculiventris nymphs, a predatory pentatomid, was evaluated with eggs of the flour moth ephestia kuehniella (pyralidae), parasitised or not by trichogramma brassicae (pupae stage). eggs of this pyralid were glued on rectangular cardboard and presented to nymphs of p. maculiventris as food. the pentatomid successfully reached adult stage when feeding on unparasitised eggs, indicating that flour moth eggs can be used as a factitious food for rearing this predator. pentatomid n ... | 2004 | 15622843 |
| effect of temperature and relative humidity on the cellular defense response of ephestia kuehniella larvae fed bacillus thuringiensis. | while maintained under all combinations of three temperatures and two rh, fifth instar larvae of the mediterranean flour moth, ephestia kuehniella were fed wheat treated with spores and crystals of bacillus thuringiensis var. kurstaki. larvae that had fed on wheat with the bacterial preparation contained higher concentrations of nodules in their haemocoel than did larvae fed on wheat without bacteria. nodule concentrations in larvae fed untreated wheat were unaffected by temperature or relative ... | 2005 | 16236308 |
| characterization of a novel vip3-type gene from bacillus thuringiensis and evidence of its presence on a large plasmid. | a novel vip3-type gene named vip3lb has been isolated from bacillus thuringiensis strain bupm95. the corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect ephestia kuehniella. the vip3lb gene was shown, for the first time, to be carried by the large plasmid containing the cry1ia genes of b. thuringiensis. the nucleotide sequence predicted a protein of 789 amino acids residues with a calculated molecular mass of 88.5kda. both nucleotide and amino acid s ... | 2005 | 15766790 |
| development and reproduction of adalia bipunctata (coleoptera: coccinellidae) on eggs of ephestia kuehniella (lepidoptera: phycitidae) and pollen. | due to growing criticism over the use of non-indigenous coccinellids, the two-spot ladybird, adalia bipunctata (l.), has enjoyed increasing attention for aphid biocontrol in europe. in the current study, eggs of the mediterranean flour moth, ephestia kuehniella zeller, whether or not supplemented with bee pollen, were evaluated as a factitious food for larvae and adults of a. bipunctata. the predator showed slower larval development and lower survival when reared on live pea aphids, acyrthosipho ... | 2005 | 16144016 |
| tolerance to bacillus thuringiensis endotoxin in immune-suppressed larvae of the flour moth ephestia kuehniella. | tolerance to bacillus thuringiensis crystal endotoxins (bt-toxins) is correlated with an elevated immune status in larvae of the flour moth ephestia kuehniella. to gain more specific information about the effector pathways involved in the protection against the toxin, we studied the effects of bt-toxin formulations in susceptible (non-induced) and tolerant (immune-induced) larvae after natural (parasitism-mediated) and chemical (tropolone-mediated) suppression of defence reactions. although mela ... | 2007 | 17499761 |
| transinfection reveals the crucial importance of wolbachia genotypes in determining the type of reproductive alteration in the host. | wolbachia , a group of endosymbiotic bacteria in arthropods, alter the reproduction of their hosts in various ways. a wolbachia strain (wsca) naturally infecting the adzuki bean borer moth ostrinia scapulalis induces male killing, while another strain (wkue) infecting the mediterranean flour moth ephestia kuehniella induces cytoplasmic incompatibility (ci) in the resident host. transinfection of wolbachia can be a powerful tool to elucidate the relative importance of wolbachia and the host in de ... | 2005 | 16174339 |
| characteristics of the drosophila paulistorum male sterility agent in a secondary host, ephestia kuehniella. | crosses among the six semispecies of drosophila paulistorum produce sterile male hybrids. this sterility is caused by an agent which has characteristics of a microorganism. it is pathogenic in a secondary host, the larvae of the mediterranean meal moth, ephestia kuehniella, and can be serially passaged in ephestia, where it is lethal. the agent was passaged back into d. paulistorum, where it induced sterility in males of a semispecies different from that of origin of the agent. infectious partic ... | 1981 | 16345886 |
| comparative study of bacillus thuringiensis cry1aa and cry1ac delta-endotoxin activation, inactivation and in situ histopathological effect in ephestia kuehniella (lepidoptera: pyralidae). | a comparative study of different steps in the mode of action of the individual bacillus thuringiensis kurstaki bns3 cry1aa and cry1ac delta-endotoxins on e. kuehniella larvae was performed in order to investigate the origin of the difference in the response of this larvae to each of the latter. proteolytic activation was shown to be one of the main steps impaired in e. kuehniella tolerance to cry1aa. the absence of two proteinase activities as well as an altered activity level observed in the ca ... | 2008 | 18040897 |
| bacillus thuringiensis as a specific, safe, and effective tool for insect pest control. | bacillus thuringiensis (bt) was first described by berliner [10] when he isolated a bacillus species from the mediterranean flour moth, anagasta kuehniella, and named it after the province thuringia in germany where the infected moth was found. although this was the first description under the name b. thuringiensis, it was not the first isolation. in 1901, a japanese biologist, ishiwata shigetane, discovered a previously undescribed bacterium as the causative agent of a disease afflicting silkwo ... | 2007 | 18051264 |
| [occurrence of the mite pyemotes sp. (acari: pyemotidae) in insect rearing in laboratory]. | pyemotidae mites have been reported as ectoparasites of a large number of arthropods, especially of insects. these mites are frequently found attacking insects in different habitats, including insect rearing. this paper reports the occurrence of pyemotes sp., ventricosus group, in colonies of tuta absoluta (meyerick) (lepidoptera: gelechiidae), anagasta kuehniella (zeller) (lepidoptera: pyralidae) and sitophilus zeamais motschulsky (coleoptera: curculionidae), in the insect rearing laboratory of ... | 2006 | 17061810 |
| [artificial diet for rearing doru luteipes (scudder) (dermaptera: forficulidae), a predator of the fall armyworm, spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae)]. | a new technique involving an artificial diet and an artificial substrate for oviposition for the rearing of the predator doru luteipes (scudder) is suggested. both adults and nymphs were maintained in petri dishes containing a transparent piece of soda straw filled with moistened cotton and the corresponding food for the biossays. the following treatments were tested: eggs of spodoptera frugiperda (j.e. smith), diatraea saccharalis fabricius (lepidoptera: pyralidae) and anagasta kuehniella zelle ... | 2007 | 17607467 |
| do anthropogenic transports facilitate stored-product pest moth dispersal? a molecular approach. | stored-product moths cause large economic damage in food processing industries and storage facilities. control of indoor pests is currently dealt with locally, and control strategies seldom include different mills or cooperative industries in joint efforts to reduce infestations. in colder climates where conditions hinder flight dispersal of stored-product moths, we hypothesize that human transport between mills will facilitate dispersal. albeit considered intuitive, this hypothesis has so far n ... | 2008 | 17713751 |
| effects of metabolic inhibitors on activity of cry1ab toxin to inhibit growth of ephestia kuehniella larvae. | although the toxins of bacillus thuringiensis berliner (bt) are frequently used to control lepidopteran pests, the tolerance or resistance of some lepidopteran moth strains may limit bt applications. in this study, insecticidal cocktails consisting of sublethal doses of cry1ab toxin and additive compounds were tested for their suppressive effect on larval relative growth rate (rgr) in ephestia kuehniella zeller under laboratory conditions. | 2008 | 18449852 |
| insecticide toxicity to trichogramma pretiosum (hymenoptera: trichogrammatidae) females and effect on descendant generation. | the effect of nine insecticides used in tomato production was evaluated on adults of two trichogramma pretiosum riley (hymenoptera: trichogrammatidae) populations from rive and afonso cláudio, espírito santo state, brazil. the experiment was developed in an acclimatized chamber at 25 +/- 1 degrees c, 70 +/- 10% relative humidity and 14 h photophase. eggs of anagasta kuehniella (lepidoptera: pyralidae), previously immersed in insecticides solutions were offered to females of both t. pretiosum pop ... | 2009 | 18931909 |
| effects of bacillus thuringiensis cry toxins on developmental and reproductive characteristics of the predator orius albidipennis (hemiptera: anthocoridae) under laboratory conditions. | the effects of cry toxins from bacillus thuringiensis (berliner) (bt) on the anthocorid orius albidipennis reuter were studied under laboratory conditions. tritrophic experiments were performed, in which orius nymphs were fed helicoverpa armigera (hübner) larvae reared on a diet with cry1ac, cry1ab, or cry2ab toxins at different concentrations (0, 1, and 10 microg/ml), when supplemented with ephestia kuehniella zeller eggs. in complementary experiments, the bt cry1ac toxin was directly fed to or ... | 2007 | 18284750 |
| purification and characterization of a trypsin inhibitor from plathymenia foliolosa seeds. | a novel trypsin inhibitor (pfti) was isolated from plathymenia foliolosa (benth.) seeds by gel filtration chromatography on a sephadex g-100, deae-sepharose, and trypsin-sepharose columns. by sdspage, pfti yielded a single band with a m(r) of 19 kda. pfti inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants (k(i)) of 4 x 10(-8) and 1.4 x 10(-6) m, respectively. pfti retained more than 50% of activity at up to 50 degrees c for 30 min, but there were 80 and 100% ... | 2008 | 18991455 |
| evidence of the involvement of e358, a498 and c571 of a new cry1ac delta-endotoxin of bacillus thuringiensis in its high insecticidal activity against ephestia kuehniella. | a new cry1ac-type gene was cloned from bacillus thuringiensis strain blb1, sequenced and expressed. the deduced amino acid sequence of the polypeptide has a predicted molecular mass of 132.186 kda. the amino acid sequence alignment of blb1 cry1ac with those of the published ones showed that this is a new delta-endotoxin. when compared with cry1ac of bacillus thuringiensis strain hd1, it was found that blb1 cry1ac harbours three mutations: v358e localized in domain ii and v498a and y571c localize ... | 2010 | 20084474 |
| a new tunisian strain of bacillus thuringiensis kurstaki having high insecticidal activity and delta-endotoxin yield. | blb1 is a new bacillus thuringiensis kurstaki strain, isolated from a tunisian soil sample. assay of toxicity of blb1 crystal proteins resulted in an lc50 of 70.32 ng of toxin per mg of flour against third instar ephestia kuehniella with confidence limits of (31.6-109.04 ng). this lc50 is less than that of the commercial strains hd1 used as a reference. the characterization of this strain by scanning transmission electron microscopy, analysis of its cry genes content by pcr-sequencing, and analy ... | 2009 | 19214476 |
| heterologous expression of bacillus thuringiensis vegetative insecticidal protein-encoding gene vip3lb in photorhabdus temperata strain k122 and oral toxicity against the lepidoptera ephestia kuehniella and spodoptera littoralis. | photorhabdus temperata and bacillus thuringiensis are entomopathogenic bacteria exhibiting toxicities against different insect larvae. vegetative insecticidal protein vip3lb is a bacillus thuringiensis insecticidal protein secreted during the vegetative growth stage exhibiting lepidopteran specificity. in this study, we focused for the first time on the heterologous expression of vip3lb gene in photorhabdus temperata strain k122. firstly, western blot analyses of whole cultures of recombinant ph ... | 2009 | 19462262 |
| [selectivity of beauveria bassiana and metarhizium anisopliae to trichogramma pretiosum riley (hymenoptera: trichogrammatidae)]. | trichogramma pretiosum riley and the entomopathogenic fungi beauveria bassiana and metarhizium anisopliae are efficient biological control agents and are thought to be used jointly. in here, we investigated if these entomopathogens could have any side-effects on t. pretiosum. therefore, 1 x 8 cards containing sterilized eggs of anagasta kuehniella (zeller) that were sprayed with 0.2 ml of b. bassiana or m. anisopliae (1.0 x 10(9) conidia/ml) were offered to a t. pretiosum female for 24h (30 card ... | 2009 | 20098929 |
| complete wo phage sequences reveal their dynamic evolutionary trajectories and putative functional elements required for integration into the wolbachia genome. | wolbachia endosymbionts are ubiquitously found in diverse insects including many medical and hygienic pests, causing a variety of reproductive phenotypes, such as cytoplasmic incompatibility, and thereby efficiently spreading in host insect populations. recently, wolbachia-mediated approaches to pest control and management have been proposed, but the application of these approaches has been hindered by the lack of genetic transformation techniques for symbiotic bacteria. here, we report the geno ... | 2009 | 19592535 |
| suitability of different lepidopteran host species for development of bracon hebetor (hymenoptera: braconidae). | bracon hebetor say (hymenoptera: braconidae) is a gregarious larval ectoparasitoid of several species of lepidoptera that are associated with stored products. the suitability of 12 potential lepidopteran host species representing four families was studied in this study for the development and reproduction of b. hebetor. the lepidoptera species used were the indianmeal moth, plodia interpunctella (hübner), mediterranean flour moth, ephestia kuehniella (zeller), almond moth, e. cautella (walker), ... | 2010 | 20388274 |
| integration of a recombinant chitinase into bacillus thuringiensis parasporal insecticidal crystal. | chitinases have been successfully used in combination with bacillus thuringiensis delta-endotoxins forming crystals in order to enhance their insecticidal activities. in this context, we opted for promoting the chitinase integration into these crystals. thus, we engineered, for the first time, a fusion protein (cdf) consisting of the chitinase chi255 and the carboxy-terminal half of cry1ac, both from b. thuringiensis subsp. kurstaki. the constructed transcriptional fusion (chi255δsp-ctcry1ac) wa ... | 2011 | 20625731 |
| characterization of tunisian bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect ephestia kuehniella. | the study of 257 crystal-producing bacillus thuringiensis isolates from bioinsecticide free soil samples collected from different sites in tunisia, was performed by pcr amplification, using six primer pairs specific for cry1, cry2, cry3, cry4, and vip3a genes, by the investigation of strain plasmid pattern, crystal morphology and delta-endotoxin content and by the assessment of insecticidal activities against the lepidopteran insect ephestia kuehniella. based on plasmid pattern study, 11 represe ... | 2010 | 20424844 |
| mortality of four stored product pests in stored wheat when exposed to doses of three entomopathogenic nematodes. | the insecticidal effect of heterorhabditis bacteriophora poinar (nematoda: heterorhabditidae), steinernema carpocapsae (weiser) (nematoda: steinernematidae), and steinernema feltiae (filipjev) (nematoda: steinernematidae) against mediterranean flour moth, ephestia kuehniella (zeller) (lepidoptera: pyralidae) (larvae), lesser grain borer, rhyzopertha dominica (f.) (coleoptera: bostrychidae) (adults), rice weevil, sitophilus oryzae (l.) (coleoptera: curculionidae) (adults), and confused flour beet ... | 2010 | 20568646 |
| insecticidal activity of the essential oils from different plants against three stored-product insects. | this study was conducted to determine the insecticidal activity of essential oils from oregano, origanum onites l. (lamiales: lamiaceae), savory, satureja thymbra l. (lamiales: lamiaceae), and myrtle, myrtus communis l. (rosales: myrtaceae) against three stored-product insects. essential oils from three species of plants were obtained by clevenger-type water distillation. the major compounds in these essential oils were identified using gas chromatography-mass spectrometry and their insecticidal ... | 2010 | 20578885 |
| wolbachia infection lowers fertile sperm transfer in a moth. | the endosymbiotic bacterium wolbachia pipientis manipulates host reproduction by rendering infected males reproductively incompatible with uninfected females (cytoplasmic incompatibility; ci). ci is believed to occur as a result of wolbachia-induced modifications to sperm during maturation, which prevent infected sperm from initiating successful zygote development when fertilizing uninfected females' eggs. however, the mechanism by which ci occurs has been little studied outside the genus drosop ... | 2010 | 20880864 |
| a trypsin inhibitor from sapindus saponaria l. seeds: purification, characterization, and activity towards pest insect digestive enzyme. | the present paper describes the purification, characterization and determination of the partial primary structure of the first trypsin inhibitor isolated from the family sapindaceae. a highly stable, potent trypsin inhibitor (ssti) was purified to homogeneity. sds-page analysis revealed that the protein consists of a two-polypeptide chain with molecular masses of approximately 15 and 3 kda. the purified inhibitor inhibited bovine trypsin at a 1:1 m ratio. kinetic analysis revealed that the prote ... | 2011 | 21127952 |
| investigation of the steps involved in the difference of susceptibility of ephestia kuehniella and spodoptera littoralis to the bacillus thuringiensis vip3aa16 toxin. | bupm95 is a bacillusthuringiensis subsp. kurstaki strain producing the vip3aa16 toxin with an interesting insecticidal activity against the lepidopteran larvae ephestia kuehniella. study of different steps in the mode of action of this vegetative insecticidal protein on the mediterranean flour moth (e. kuehniella) was carried out in the aim to investigate the origin of the higher susceptibility of this insect to vip3aa16 toxin compared to that of the egyptian cotton leaf worm spodoptera littoral ... | 2011 | 21600212 |
| amino acids y229 and f603 are involved in bacillus thuringiensis cry1ac delta-endotoxin stability and toxicity. | bacillus thuringiensis cry1ac toxin shares structurally five conserved blocs with the other delta-endotoxins. in order to study the role of some amino acids belonging to these regions, two mutations, y(229) p and f(603) s, have been introduced separately and respectively in blocs 2 and 5. the resulting mutant proteins cry1ac'1 and cry1ac'3 were affected in their stability and crystallization. both of them lost their toxicity to the lepidopteran larvae ephestia kuehniella. unlike cry1ac'1, cry1ac ... | 2012 | 22268917 |
| overcoming the production limitations of photorhabdus temperata ssp. temperata strain k122 bioinsecticides in low-cost medium. | for low-cost production of photorhabdus temperata ssp. temperata strain k122 bioinsecticide, a cheap complex medium was optimized. diluted seawater was used as the source of micronutrients, especially sodium chloride, involved in the improvement of cell density, culturability and oral toxicity of the bacterium p. temperata against ephestia kuehniella larvae. thus, the new formulated medium was composed only of 10 g/l of soya bean meal, used as the carbon and nitrogen main source, mixed in sevenf ... | 2011 | 21656156 |
| insecticide selectivity to two species of trichogramma in three different hosts, as determined by iobc/wprs methodology. | background: the use of trichogramma species is a potential key strategy in integrated pest management. however, its effectiveness depends on the use of chemicals that do not interfere with parasitism and parasite population growth. here, a study was made of the effects of synthetic insecticides on trichogramma pretiosum and trichogramma exiguum in different hosts (ephestia kuehniella, plutella xylostella and spodoptera frugiperda) and the influence of international organisation for biological co ... | 2011 | 21858909 |
| chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of mediterranean pinus brutia and pinus pinea resin essential oils. | essential oils of the resins of pinus brutia and pinus pinea were evaluated for their biological potential. essential oils were characterized using gc-ms and gc/fid. in vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. the chemical profile of the essential oils of the resins of p. pinea and p. brutia included mainly α-pinene (21.39% and 25.40%), β-pinene (9.68% and 9.69%), and caryophyllene ( ... | 2014 | 25556061 |
| influence of ephestia kuehniella stage larvae on the potency of bacillus thuringiensis cry1aa delta-endotoxin. | the economically important crop pest ephestia kuehniella was tested at two stages of larval development for susceptibility to bacillus thuringiensis cry1aa toxin. bioassays showed that toxicity decreased during the development of larvae stage. in fact, cry1aa toxins from bns3-cry(-) (pht-cry1aa) showed low toxicity against the first-instar larvae (l1) with a lc50 value of about 421.02μg/g of diet and was not toxic against the fifth-instar (l5), comparing to the blb1 toxins used as positive contr ... | 2017 | 28364809 |
| vegetative insecticidal protein of bacillus thuringiensis blb459 and its efficiency against lepidoptera. | bacillus thuringiensis strain blb459 supernatant showed a promising activity against lepidopteran pests with extremely damages in the larvae midgut. investigations of the genes that encode secreted toxin demonstrated that this strain harbored a vip3-type gene named vip3(459). based on its original nucleotide and amino acid sequences, this gene was cloned into pet-14b vector and overexpressed in escherichia coli. the expressed protein was purified and tested against different insects and interest ... | 2017 | 28223048 |
| three homopteran pests of citrus as prey for the convergent lady beetle: suitability and preference. | the convergent lady beetle, hippodamia convergens guérin-méneville (coleoptera: coccinellidae), is an important predator of soft-bodied insect pests in many regions of the united states, but generally uncommon in florida citrus. certain citrus producers in florida recently initiated releases of commercially available h. convergens from california against the asian citrus psyllid diaphorina citri kuwayama, vector of huanglongbing or citrus greening disease. however, there is little information o ... | 2011 | 22217767 |
| factors influencing supercooling capacity of the koinobiont endoparasitoid venturia canescens (hymenoptera: ichneumonidae). | venturia canescens is a parthenogenetic koinobiont endoparasitoid of several pyralid moth larvae that are major pests of stored products. low temperatures have been extensively used to control stored-product insects as an alternative method to the application of traditional pesticides. however, most studies have focused on the cold hardiness profile of the major stored-product pests. the objective of this study was to investigate how factors such as age, food, availability of host and acclimatio ... | 2013 | 23913517 |
| mobility and electric charge of screening pigment granules in the superposition eye of ephestia kuehniella z. | the mobility and the electric charge of screening pigment granules of the mealmoth superposition eye were determined electrophoretically in buffer solutions. in potassium phosphate buffer the mobility of the negatively charged granules is linearly dependent on the ph in the range from 4.8 to 7.7, and in veronal buffer from ph = 2.3 to ph = 7.5. at ph = 6.6 the values of the effective charge per granule vary between 9.4 x 10(-17) c and 2.0 x 10(-16) c, those of the real charge between 2.4 x 10(-1 ... | 2013 | 3837106 |
| ephestia kuehniella tolerance to bacillus thuringiensis cry1aa is associated with reduced oligomer formation. | the basis of the different susceptibility of ephestia kuehniella to the cry1aa and cry1ac δ-endotoxins from bacillus thuringiensis kurstaki bns3 was studied. both toxins bound specifically to the bbmv of e. kuehniella. the result of the ligand blot showed that cry1ac bound to three putative receptors of about 100, 65 and 80 kda and cry1aa interacted only with a 100 kda protein. pronase digestion of the bbmv-bound toxins was used to analyze the toxin insertion. both toxins inserted into the bbmv ... | 2017 | 27888109 |
| toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of bacillus thuringiensis crystal protein cry2aa. | bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. improving their efficacy is of great benefit. here, an improvement in cry2aa δ-endotoxin toxicity was attempted via a cry gene over expression system using p20 from b. thuringiensis israelensis. | 2016 | 26910489 |
| cry1ac toxicity enhancement towards lepidopteran pest ephestia kuehniella through its protection against excessive proteolysis. | bacillus thuringiensis has been extensively used in agroecosystems for four decades due to its high specific toxicity. strategies based on b. thuringiensis proteins combinations for the improvement of its activity present an important focus for biopesticides development. however, the widespread use of b. thuringiensis δ-endotoxins has often been challenged by a lack of understanding of the target insect physiology as well as its midgut biochemistry. in the present investigation, we have evidence ... | 2016 | 27452929 |
| effect of adding amino acids residues in n- and c-terminus of vip3aa16 (l121i) toxin. | to study the importance of n- and c-terminus of bacillus thuringiensis vip3aa16 (l121i) toxin (88 kda), a number of mutants were generated. the addition of two (2r: rs) or eleven (11r: rsrpghhhhhh) amino acid residues at the vip3aa16 (l121i) c-terminus allowed to an unappropriated folding illustrated by the abundant presence of the 62 kda proteolytic form. the produced vip3aa16 (l121i) full length form was less detected when increasing the number of amino acids residues in the c-terminus. bioass ... | 2016 | 26876111 |
| expression of cry1ab gene from a novel bacillus thuringiensis strain sy49-1 active on pest insects. | in this study, the cry1ab gene of previously characterized and lepidoptera-, diptera-, and coleoptera-active bacillus thuringiensis sy49-1 strain was cloned, expressed and individually tested on ephestia kuehniella (lepidoptera: pyralidae) and plodia interpunctella (lepidoptera: pyralidae) larvae. pet-cry1ab plasmids were constructed by ligating the cry1ab into pet28a (+) expression vector. constructed plasmids were transferred to an escherichia coli bl21 (de3) strain rendered competent with cac ... | 2017 | 27143037 |
| a promising hd133-like strain of bacillus thuringiensis with dual efficiency to the two lepidopteran pests: spodoptera littoralis (noctuidae) and ephestia kuehniella (pyralidae). | isolation and identification of new strains with wide variety of target pests is an ever growing field. in this paper, a screening of 260 strains from tunisian soil samples was conducted by dot-blot and pcr-sequencing analysis. the screening was done on the basis of the possession of cry1d-type genes and was followed by the evaluation of the insecticidal activity against spodoptera littoralis. blb250 strain showed an lc50 value (56.2 μg g(-1)) against s. littoralis lower than those of the two ba ... | 2016 | 27130040 |
| isolation and characterization of a new bacillus thuringiensis strain with a promising toxicity against lepidopteran pests. | insecticides derived from bacillus thuringiensis are gaining worldwide importance as environmentally desirable alternatives to chemicals for the control of pests in public health and agriculture. isolation and characterization of new strains with higher and broader spectrum of activity is an ever growing field. in the present work, a novel tunisian b. thuringiensis isolate named blb459 was characterized and electrophoresis assay showed that among a collection of 200 b. thuringiensis strains, the ... | 2017 | 27242138 |
| molecular characterisation of bacillus thuringiensis strain meb4 highly toxic to the mediterranean flour moth ephestia kuehniella zeller (lepidoptera: pyralidae). | cry2 proteins play an essential role in current bacillus thuringiensis (bt) applications and in the prevention of insect resistance to cry1a toxins. this paper reports on the screening and characterisation of novel bt strains harbouring effective cry2a-type genes and higher insecticidal activity to ephestia kuehniella. | 2016 | 26103535 |
| isolation and characterization of a new bacillus thuringiensis strain lip harboring a new cry1aa gene highly toxic to ephestia kuehniella (lepidoptera: pyralidae) larvae. | the aim of this study was to characterize new bacillus thuringiensis strains that have a potent insecticidal activity against ephestia kuehniella larvae. strains harboring cry1a genes were tested for their toxicity, and the lip strain showed a higher insecticidal activity compared to that of the reference strain hd1 (lc50 of lip and hd1 were 33.27 and 128.61 μg toxin/g semolina, respectively). b. thuringiensis lip harbors and expresses cry1aa, cry1ab, cry1ac, cry1ad and cry2a. dna sequencing rev ... | 2014 | 24715255 |
| efficacy of olive mill wastewater for protecting bacillus thuringiensis formulation from uv radiations. | the effectiveness of 10 low-cost uv-absorbers in protecting bacillus thuringiensis subsp. kurstaki blb1 toxins against inactivation by uv-a and uv-b irradiation was evaluated in this study. among them, two by-products, molasses and olive mill wastewater (omw) were selected for further studies. they were tested at different concentrations of 0.05, 0.1, 0.15 and 0.2% using the para-aminobenzoic acid (paba) as a common uv protectant. interestingly, addition of paba and omw to blb1 formulations was ... | 2014 | 25093915 |
| impact of q139r substitution of meb4-cry2aa toxin on its stability, accessibility and toxicity against ephestia kuehniella. | the bacillus thuringiensis subsp. kurstaki strain meb4 was previously found to be highly toxic to ephestia kuehniella. sds-page analysis of the recombinant strain dh5α (pbs-cry2aa-meb4) showed that cry2aa-meb4 delta-endotoxins were forming inclusion bodies, and were 2.75 fold more toxic towards e. kuehniella than those of cry2aa-bns3. besides to the 65kda active toxin, proteolysis activation of cry2aa-bns3 protein with e. kuehniella midgut juice generated an extra proteolysis form of 49kda, whic ... | 2015 | 26321422 |
| development of chrysoperla carnea (stephens) (neuroptera: chrysopidae) on pollen from bt-transgenic and conventional maize. | maize (zea mays) pollen is highly nutritious and can be used by predatory arthropods to supplement or replace a carnivorous diet. we demonstrate that maize pollen can be utilized by larvae of the green lacewing, chrysoperla carnea (neuroptera: chrysopidae) under laboratory conditions. complete development on maize pollen was not possible, but 25% of neonates reached the third instar. when only one instar was fed with pollen and the other two instars with eggs of ephestia kuehniella (lepidoptera: ... | 2014 | 25082074 |
| influence of diet on fecundity, immune defense and content of 2-isopropyl-3-methoxypyrazine in harmonia axyridis pallas. | food type can affect all functional aspects of an insect's life. we investigated the effects of different diet regimes on life history parameters of the ladybird beetle harmonia axyridis. furthermore, we tested the importance of elytral color, sex, and diet on chemical and immune defense in this species. we also compared hemolymph from cohorts of h. axyridis and coccinella septempunctata (coleoptera: coccinellidae) fed different diets to examine effects on the 2-isopropyl-3-methoxypyrazine (ipmp ... | 2012 | 22648506 |
| new bacillus thuringiensis toxin combinations for biological control of lepidopteran larvae. | cyt1aa from bacillus thuringiensis israelensis is known by its synergistical activity with b. thuringiensis and bacillus sphaericus toxins. it is able to improve dipteran specific toxins activity and can prevent or overcome larval resistance to those proteins. the objective of the current study was to investigate the possible improvement of larvicidal activity of b. thuringiensis kurstaki expressing heterogeneous proteins cyt1a and p20. cyt1a98 and p20 genes encoding the cytolytic protein (cyt1a ... | 2014 | 24444881 |
| response of larval ephestia kuehniella (lepidoptera: pyralidae) to individual bacillus thuringiensis kurstaki toxins mixed with xenorhabdus nematophila. | bacillus thuringiensis kurstaki strain bns3 produces parasporal crystals formed by cry1aa, cry1ac and cry2aa delta-endotoxins. in a previous work, we showed that the latter exhibited individually, a weak insecticidal activity against ephestia kuehniella. in order to improve their toxicities, we studied the combined effect of each delta-endotoxin with x. nematophila cells on e. kuehniella larvae growth. xenorhabdus cells were used in combination with spore crystal mixture of the wild strain bns3, ... | 2013 | 23747825 |
| screening and identification of a bacillus thuringiensis strain s1/4 with large and efficient insecticidal activities. | the bacterium bacillus thuringiensis was recognized for its entomopathogenic activities related to cry and cyt proteins forming the δ-endotoxins and some extracellular activities like the vegetative insecticidal proteins (vip) and cry1i. these activities may act specifically against diverse organisms and some of them typically characterize each strain. here, we screened a set of 212 b. thuringiensis strains to search the higher insecticidal activities. these strains had bipyramidal and cubic cry ... | 2013 | 22915162 |
| artificial and factitious foods support the development and reproduction of the predatory mite amblyseius swirskii. | the generalist predatory mite amblyseius swirskii athias-henriot (acari: phytoseiidae) was reared on ephestia kuehniella zeller eggs (lepidoptera: pyralidae), decapsulated dry cysts of the brine shrimp artemia franciscana kellogg (anostraca: artemiidae), and on meridic artificial diets (composed of honey, sucrose, tryptone, yeast extract, and egg yolk) supplemented with pupal hemolymph of the chinese oak silkworm antheraea pernyi (guérin-méneville) (lepidoptera: saturniidae) (ad1), with e. kuehn ... | 2014 | 24154947 |
| development and reproduction of chrysoperla externa (hagen) (neuroptera: chrysopidae) fed on myzus persicae (sulzer) (hemiptera: aphididae) vectoring potato leafroll virus (plrv). | the aim of this study was to evaluate the biological parameters of chrysoperla externa (hagen) while feeding on myzus persicae (sulzer) acting as a vector of potato leafroll virus (plrv). in laboratory experiments, three different diets were offered ad libitum to c. externa during the larval period: m. persicae fed on plrv-infected potato leaves, m. persicae fed on uninfected potato leaves, and eggs of the factitious prey anagasta kuehniella (zeller) as the control. the following parameters were ... | 2015 | 26395997 |
| effect of nymphal diet on adult predation behavior in orius majusculus (heteroptera: anthocoridae). | the predatory bug orius majusculus (reuter) was reared on 2 different diets during the nymphal stages. the 1st group was exclusively offered eggs of ephestia kuehniella zeller (lepidoptera: pyralidae), a standard diet for o. majusculus production. the 2nd group was exclusively offered 4th instars of the pea aphid, acyrtosiphon pisum (harris). subsequently, adult predatory behavior in experimental arenas containing a. pisium was recorded using 2 video cameras. one camera permitted observation of ... | 2000 | 10826169 |
| feeding history affects intraguild interactions between harmonia axyridis (coleoptera: coccinellidae) and episyrphus balteatus (diptera: syrphidae). | while the effect of several factors such as predator and prey size, morphology and developmental stage on intraguild predation (igp) is widely investigated, little is known about the influence of diet on the occurrence and outcome of igp. in the present study, the effect of the diet experienced during larval development on igp between the ladybird harmonia axyridis and the syrphid episyrphus balteatus is investigated. four diets were tested for h. axyridis: eggs of the mediterranean flour moth e ... | 2015 | 26030267 |
| nutritional plasticity of the predatory ladybeetle harmonia axyridis (coleoptera: coccinellidae): comparison between natural and substitution prey. | the predatory coccinellid harmonia axyridis is a polyphagous species, efficient at controlling certain aphid species and already commercialized in europe for that purpose. the complete development of this predator can be accomplished using the aphid acyrthosiphon pisum or ephestia kuehniella eggs as substitution prey. biochemical analyses were conducted on the proteins, lipids, and carbohydrates of these two different prey species. e. kuehniella eggs were 2 times richer in amino acids than a. pi ... | 2003 | 12529863 |
| moisture source and diet affect development and reproduction of orius thripoborus and orius naivashae, two predatory anthocorids from southern africa. | the effect of moisture source and diet on the development and reproduction of the pirate bugs, orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) was examined in the laboratory. both species had been collected in and around sugarcane fields in south africa. supplementing eggs of the flour moth ephestia kuehniella (zeller) (lepidoptera: pyralidae) with a green bean pod as a moisture source yielded better nymphal survival and faster development, as compared with free ... | 2012 | 22935002 |
| adenanthera pavonina trypsin inhibitor retard growth of anagasta kuehniella (lepidoptera: pyralidae). | anagasta kuehniella is a polyphagous pest that feeds on a wide variety of stored products. the possible roles suggested for seed proteinase inhibitors include the function as a part of the plant defensive system against pest via inhibition of their proteolytic enzymes. in this study, a trypsin inhibitor (apti) was purified from adenanthera pavonina seed and was tested for insect growth regulatory effect. the chronic ingestion of apti did result in a significant reduction in larval survival and w ... | 2010 | 20235154 |
| temperature-dependent development of the two-spotted ladybeetle, adalia bipunctata, on the green peach aphid, myzus persicae, and a factitious food under constant temperatures. | the ability of a natural enemy to tolerate a wide temperature range is a critical factor in the evaluation of its suitability as a biological control agent. in the current study, temperature-dependent development of the two-spotted ladybeetle a. bipunctata l. (coleoptera: coccinellidae) was evaluated on myzus persicae (sulzer) (hemiptera: aphididae) and a factitious food consisting of moist bee pollen and ephestia kuehniella zeller (lepidoptera: pyralidae) eggs under six constant temperatures ra ... | 2010 | 20879918 |
| selectivity of neem to trichogramma pretiosum riley and trichogrammatoidea annulata de santis (hymenoptera: trichogrammatidae). | trichogramma pretiosum riley and trichogrammatoidea annulata de santis are commonly found in avocado and persimmon orchards in northern parana state. however, their abundance depends on whether insecticides are used or not to control the key lepidopteran pests stenoma catenifer (wals.) (lepidoptera: elachistidae) and hypocala andremona (stoll) (lepidoptera: noctuidae), respectively. the aim of this work was to evaluate the effects of an aqueous neem seed extract (anse) at 15, 3 and 1.5%, and of ... | 2016 | 21271068 |
| life-history traits of macrolophus pygmaeus with different prey foods. | macrolophus pygmaeus rambur (hemiptera: miridae) is a generalist predatory mirid widely used in augmentative biological control of various insect pests in greenhouse tomato production in europe, including the invasive tomato leafminer, tuta absoluta (meyrick) (lepidoptera, gelechiidae). however, its biocontrol efficacy often relies on the presence of alternative prey. the present study aimed at evaluating the effect of various prey foods (ephestia kuehniella eggs, bemisia tabaci nymphs, tuta abs ... | 2016 | 27870857 |
| molecular characterization of ephestia kuehniella (lepidoptera: pyralidae) transferrin and its response to parasitoid venturia canescens (hymenoptera: ichneumonidae gravenhorst). | in the present study, we characterized a full-length cdna encoding a putative iron-binding protein transferrin from the lepidopteran mediterranean flour moth (ektrf, 2397 bp). the putative ektrf is 683 amino acids with a molecular mass of approximately 76 kda. the deduced amino acid sequence showed significant homology with other insect transferrins from chilo suppressalis (76%), galleria mellonella (75%), plutella xylostella (72%), manduca sexta (74%), bombyx mori (73%), spodoptera litura and ( ... | 2012 | 22229520 |
| influence of photoperiod on orius thyestes herring (hemiptera: anthocoridae) reproduction and longevity. | several species of orius wolff are used in biological control of thrips in protected cultivations in temperate regions, but some of them show reproductive diapause, compromising the efficiency of these agents of biological control. there are no reports on the biology of the neotropical species orius thyestes herring under different environmental conditions. the purpose of this work was to investigate the influence of photoperiod on reproduction and longevity of this predator. nymphs were kept in ... | 2006 | 17061797 |
| moth sex chromatin probed by comparative genomic hybridization (cgh). | abstract: comparative genomic hybridization (cgh) with a probe mixture of differently labeled genomic dna from females and males highlighted the w chromosomes in mitotic plates and the w chromatin in polyploid interphase nuclei of the silkworm bombyx mori, the flour moth ephestia kuehniella, and the wax moth galleria mellonella. the overproportionate fluorescence signal indicated an accumulation of repetitive sequences in the respective w chromosomes. measurements of the fluorescence signals rev ... | 2003 | 12723050 |
| bowman-birk proteinase inhibitor from clitoria fairchildiana seeds: isolation, biochemical properties and insecticidal potential. | herein described is the biochemical characterisation, including in vitro and in vivo assays, for a proteinase inhibitor purified from clitoria fairchildiana seeds (cfpi). purification was performed by hydrophobic interaction and gel filtration chromatography. kinetic studies of the purified inhibitor showed a competitive-type inhibitory activity against bovine trypsin and chymotrypsin, with an inhibition stoichiometry of 1:1 for both enzymes. the inhibition constants against trypsin and chymotry ... | 2015 | 26330217 |
| chemical composition and fumigant toxicity of some essential oils against ephestia kuehniella. | the chemical constituents of some essential oils extracted from aromatic plants (savory, satureja thymbra l.; turkish oregano, origanum onites l.; myrtle, myrtus communis l.; marjoram, origanum majorana l.; laurel, laurus nobilis l.; lemon, citrus limon l.; sticky goosefoot, chenopodium botrys l.; and tansy, tanecetum armenum [dc.] suchultz bip.) were analyzed by gas chromatography-mass spectrometry. fumigant toxicity of volatile compounds was tested against mediterranean flour moth, ephestia ku ... | 2011 | 21882685 |
| development of an early-tier laboratory bioassay for assessing the impact of orally-active insecticidal compounds on larvae of coccinella septempunctata (coleoptera: coccinellidae). | early-tier studies are the initial step in the environmental risk assessment of genetically engineered plants on nontarget arthropods. they are conducted in the laboratory where surrogate species are exposed to higher concentrations of the arthropod-active compound than those expected to occur in the field. thus, early-tier tests provide robust data and allow to make general conclusions about the susceptibility of the surrogate to the test substance. we have developed an early-tier test for asse ... | 2012 | 23321119 |
| [parasitism capacity of trichogramma pratissolii querino & zucchi (hymenoptera: trichogrammatidae) on alternative hosts, under different temperatures]. | the successful use of trichogramma as biocontrol agent depends on its mass production in laboratory, a fundamental step for any biological control program among other factors. this work investigated the parasitism capacity of trichogramma pratissolii querino & zuchi (hymenoptera: trichogrammatidae), a new recorded trichogramma species, parasitizing eggs of anagasta kuehniella (zeller) and corcyra cephalonica (stainton) (lepidoptera: pyralidae) under the temperatures of 15, 18, 21, 24, 27, 30 and ... | 2007 | 17420865 |
| [biology and thermal requirements trichogramma pratissolii querino & zucchi (hymenoptera: trichogrammatidae) on alternative hosts]. | the objective of this work was to study the biology and determine the thermal requirements of trichogramma pratissolii querino & zucchi reared on anagasta kuehniella zeller (lepidoptera: pyralidae) and corcyra cephalonica stainton (lepidoptera: pyralidae) at 15, 18, 21, 24, 27, 30 and 33 masculinec. the highest percentage of emergence of t. pratissolii was obtained at 27 masculinec on a. kuehniella and from 24 masculinec to 30 masculinec on c. cephalonica. the lower threshold temperature (tb) an ... | 2008 | 18575699 |
| insecticidal action of annona coriacea lectin against the flour moth anagasta kuehniella and the rice moth corcyra cephalonica (lepidoptera: pyralidae). | annona coriacea lectin (aclec) was tested for insecticidal activity against larvae of two pyralid moths, anagasta kuehniella and corcyra cephalonica. aclec produced approximately 50% mortality and mass loss in a. kuehniella larvae when incorporated into an artificial diet at levels of 1.5% and 1.0% (w/w), respectively. in contrast, the inclusion of up to 2% aclec in the diet did not significantly decrease the survival or weight of c. cephalonica larvae. the nutritional indices for a. kuehniella ... | 2007 | 17561444 |
| properties of a kunitz-type trypsin inhibitor from delonix regia seeds against digestive proteinases of anagasta kuehniella (z.) and corcyra cephalonica (s.) (lepidoptera: pyralidae). | drti was effective against trypsin-like enzymes from a. kuehniella and c. cephalonica, however an artificial diet was insufficient to affect the survival and body weight of either insect. the inhibitor stimulated chymotrypsin-like enzymes and probably induced the synthesis of enzymes insensitive to tlck in neonate larvae. | 2009 | 20001900 |
| biological aspects of eriopis connexa (germar) (coleoptera: coccinellidae) fed on different insect pests of maize (zea mays l.) and sorghum [sorghum bicolor l. (moench.)]. | eriopis connexa (germar) (coleoptera: coccinellidae) occurs in several countries of south america and its mass rearing is important for biological control programmes. this work evaluated biological aspects of e. connexa larva fed on eggs of anagasta kuehniella (zeller) (lepidoptera: pyralidae) and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae) frozen for one day, fresh eggs of diatraea saccharalis (fabricius) (lepidoptera: pyralidae), s. frugiperda newly-hatched caterpillars, nymph ... | 2013 | 23917572 |
| two-spotted ladybeetle adalia bipunctata l. (coleoptera: coccinellidae): a commercially available predator to control asian citrus psyllid diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid diaphorina citri kuwayama (hemiptera: liviidae) is an economically important pest of citrus because it serves as a vector of the causal pathogens of huanglongbing (hlb) also known as citrus greening disease. the increased use of insecticides for control of d. citri negatively impacts several natural enemies including some effective ladybeetle species which are not available commercially. the two-spotted ladybeetle, adalia bipunctata (coleoptera: coccinellidae) is found i ... | 2016 | 27631730 |
| relative toxicity of two aphicides to hippodamia convergens (coleoptera: coccinellidae): implications for integrated management of sugarcane aphid, melanaphis sacchari (hemiptera: aphididae). | flupyradifurone and sulfoxaflor present novel insecticide chemistries with particular efficacy against aphids, and the recent emergence of sugarcane aphid, melanaphis sacchari (zehntner), as a pest of sorghum in the united states has resulted in their widespread use. we examined their toxicity to hippodamia convergens guerin-meneville, an important aphid biocontrol agent. we exposed beetles to topical applications of the field rate (fr) of these insecticides, fed them contaminated food (eggs of ... | 2017 | 28039423 |
| herbicide toxicity, selectivity and hormesis of nicosulfuron on 10 trichogrammatidae (hymenoptera) species parasitizing anagasta ( = ephestia) kuehniella (lepidoptera: pyralidae) eggs. | selective agrochemicals including herbicides that do not affect non-target organisms such as natural enemies are important in the integrated pest management (ipm) programs. the aim of this study was to evaluate the herbicide toxicity, selectivity and hormesis of nicosulfuron, recommended for the corn zea mays l. (poaceae) crop, on 10 trichogrammatidae (hymenoptera) species. a female of each trichogramma spp. or trichogrammatoidea annulata de santis, 1972 was individually placed in plastic test t ... | 2017 | 27726483 |
| extending the "ecology of fear" beyond prey: reciprocal nonconsumptive effects among competing aphid predators. | nonconsumptive effects of predators on prey are well known, but similar effects among competing predators are not. aphidophagous insect larvae are notorious for cannibalism and intraguild predation, as they compete for aggregated but ephemeral prey. we tested for indirect effects of competitors on the development of coleomegilla maculata degeer and hippodamia convergens guerin-meneville (coleoptera: coccinellidae), and a green lacewing, chrysoperla carnea stephens (neuroptera: chrysopidae), with ... | 2016 | 27686817 |
| oviposition model of ephestia kuehniella (lepidoptera: pyralidae). | in this study, we examined the fecundity, survival, and adult longevity of ephestia kuehniella (zeller) at different temperatures (15, 17.5, 20, 22.5, 25, 27.5, and 30 °c), and developed a model of oviposition. the fecundity, survival, and adult longevity of e. kuehniella were affected by temperature. longevity reduced from 15.7 d at 15 °c to 8.79 d at 30 °c. total fecundity was lowest (160.54 eggs) at 30 °c and highest (351.75 eggs) at 25 °c. the relationship between temperature and total fecun ... | 2016 | 27567220 |
| rearing chrysoperla externa larvae on artificial diets. | we tested three artificial diets for rearing larvae of chrysoperla externa (hagen) (neuroptera: chrysopidae), aiming at reducing the production costs of this predator. two of the diets come from studies with other species of lacewings, and the third is a modification described in this paper. all diets were based on animal protein and were supplied to 2nd and 3rd instar larvae, whereas 1st instar larvae received eggs of anagasta kuehniella (zeller) (lepidoptera: pyralidae). we evaluated the preim ... | 2017 | 27530223 |