| rapid diagnosis of anaerobic infections by direct gas-liquid chromatography of clinical speciments. | current methods to isolate and identify anaerobic bacteria are laborious and time consuming. it was postulated that the short-chain fatty acids (scfa) produced by these organisms might serve as microbial markers in clinical material. 98 specimens of pus or serous fluid were analyzed by gas-liquid chromatography, and findings were compared with culture results. good correlations were found for the recovery of anaerobic gram-negative bacilli and the presence of isobutyric, butyric, and succinic ac ... | 1976 | 1254729 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| role of anaerobic bacteria in bite-wound infections. | the etiologic agents usually involved in wound infections due to human or animal bites are the aerobic skin flora of the victim, e.g., staphylococcus aureus, and/or the aerobic oral flora of the biter, e.g., pasteurella multocida. while anaerobic bacteria are predominant in the normal oral flora of humans and animals, their importance in the pathogenesis of bite-wound infections has not been stressed. most investigators in this field have either not cultured these wounds for anaerobic bacteria o ... | 1984 | 6372026 |
| phylogenetic placement of dialister pneumosintes (formerly bacteroides pneumosintes) within the sporomusa subbranch of the clostridium subphylum of the gram-positive bacteria. | the nucleotide sequence of the 16s rrna gene of the type strain of dialister pneumosintes was determined. phylogenetic analysis revealed that this species belongs to the sporomusa branch of the clostridium subphylum of the gram-positive bacteria and should therefore be excluded from the family bacteroidaceae. within this branch, which encompasses several other gram-negative taxa, such as acidaminococcus, pectinatus, phascolarcobacterium, quinella, selenomonas, and zymophilus, dialister showed a ... | 1995 | 7537075 |
| [normal flora of the human conjunctiva: examination of 135 persons of various ages]. | conjunctival flora is a prime suspect in searching for the cause of post-traumatic or post-operative ocular infections. better information on its individual composition is therefore desirable. | 1994 | 7869685 |
| changes in root canal microbiota during the development of rat periapical lesions. | periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. in the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. the mandibular first molars of sprague-dawley rats were exposed on day 0. the teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the m ... | 1994 | 7936717 |
| oribaculum catoniae gen. nov., sp. nov.; catonella morbi gen. nov., sp. nov.; hallella seregens gen. nov., sp. nov.; johnsonella ignava gen. nov., sp. nov.; and dialister pneumosintes gen. nov., comb. nov., nom. rev., anaerobic gram-negative bacilli from the human gingival crevice. | the following four new species of anaerobic gram-negative bacilli isolated from the human gingival crevice are described: oribaculum catoniae, with atcc 51270 as the type strain; catonella morbi, with atcc 51271 as the type strain; hallella seregens, with atcc 51272 as the type strain; and johnsonella ignava, with atcc 51276 as the type strain. c. morbi is associated with periodontitis. h. seregens and j. ignava are associated with gingivitis and periodontitis. o. catoniae has been isolated from ... | 1994 | 8186083 |
| molecular identification of dialister pneumosintes in subgingival plaque of humans. | dialister pneumosintes is a nonfermentative, anaerobic, gram-negative rod that grows with small, circular, transparent, shiny, smooth colonies on blood agar. even though d. pneumosintes has been recovered from deep periodontal pockets, little is known about the relationship between the organism and destructive periodontal disease. this study describes a rapid pcr method to identify d. pneumosintes in periodontal samples. the pcr identification method detected as little as 10 pg of d. pneumosinte ... | 2000 | 10921975 |
| importance of dialister pneumosintes in human periodontitis. | anaerobic bacteria play important roles in the pathogenesis of human periodontitis. this study examined the relationship between a potentially new periodontopathic bacterium dialister pneumosintes and periodontal disease. a total of 73 women and 62 men aged 18 to 86 years participated in the study. using a 16s rrna polymerase chain reaction identification method, the presence of d. pneumosintes was determined in paper-point samples from periodontal pockets of 105 periodontitis and 30 gingivitis ... | 2000 | 11154414 |
| herpes viruses and periodontopathic bacteria in early-onset periodontitis. | this study examined the occurrence of human herpes viruses and suspected periodontopathic bacteria in early-onset periodontitis patients who experienced progressive disease in at least 2 periodontal sites during the maintenance phase of therapy. | 2001 | 11493359 |
| characterisation of eubacterium-like strains isolated from oral infections. | the genus eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. as a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. in this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the ... | 2001 | 11699590 |
| dialister pneumosintes, a new putative periodontal pathogen. | recent studies have implicated dialister pneumosintes as a candidate periodontal pathogen. this study determined the association of subgingival d. pneumosintes with demographic variables (age and gender) and the presence of subgingival periodontopathic bacteroides forsythus and porphyromonas gingivalis. microbial identification by established pcr techniques was performed in samples from 149 periodontitis patients. subgingival d. pneumosintes occurred with significantly higher prevalence in older ... | 2002 | 11842941 |
| dialister pneumosintes associated with human brain abscesses. | in this report, we review two cases of brain infection due to dialister pneumosintes in previously healthy patients. the bacterium was isolated from the first patient by blood culture and directly from a brain abscess in the second patient. in both cases, the infection was suspected to be of nasopharyngeal or dental origin. the patients had favorable outcomes following surgical debridement and antibiotic treatment. after in vitro amplification and partial sequencing of the 16s rrna gene, two str ... | 2002 | 12354905 |
| dialister pneumosintes can be a suspected endodontic pathogen. | dialister pneumosintes is a nonmotile, nonfermentative, non-spore-forming, obligately anaerobic, gram-negative bacillus that has been associated with some infections in the human body. a species-specific nested polymerase chain reaction assay was used to investigate the occurrence of d pneumosintes in primary root canal infections. | 2002 | 12374926 |
| cytomegalovirus periodontal presence is associated with subgingival dialister pneumosintes and alveolar bone loss. | destructive periodontal disease is associated with human cytomegalovirus (hcmv), epstein-barr type 1 virus (ebv-1) and other members of the herpesviridae family as well as with various gram-negative anaerobic bacteria, including the dialister pneumosintes species. this study aimed to determine possible interrelationships between periodontal hcmv, ebv-1, herpes simplex virus and d. pneumosintes, and relate the microbiological findings to periodontitis clinical status. sixteen subjects each contri ... | 2002 | 12485328 |
| herpesviral-bacterial interactions in aggressive periodontitis. | human cytomegalovirus, epstein-barr virus and herpesvirus co-infections occur with significantly higher frequency in actively progressing than in stable periodontitis sites of adolescents and young adults. also, periodontal presence of cytomegalovirus and epstein-barr virus is associated with increased occurrence of subgingival porphyromonas gingivalis, bacteroides forsythus, dialister pneumosintes, prevotella intermedia, prevotella nigrescens, treponema denticola and actinobacillus actinomycete ... | 2003 | 12716334 |
| pcr methodology as a valuable tool for identification of endodontic pathogens. | this paper reviews the principles of polymerase chain reaction (pcr) methodology, its application in identification of endodontic pathogens and the perspectives regarding the knowledge to be reached with the use of this highly sensitive, specific and accurate methodology as a microbial identification test. | 2003 | 12799118 |
| positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections. | dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ... | 2003 | 12877258 |
| the chemical composition of dialister pneumosintes and the significance of the studies of small eubacteria. | | 1962 | 14020486 |
| dialister invisus sp. nov., isolated from the human oral cavity. | six strains of anaerobic, gram-negative coccobacilli isolated from the root canals of patients with endodontic infections (five strains) and from a deep periodontal pocket (one strain) were subjected to a comprehensive range of phenotypic and genetic tests and were found to comprise a homogeneous group. following 16s rrna gene sequence analysis, they were found to be most closely related to dialister pneumosintes, with 93 % sequence similarity between the two taxa. a novel species, dialister inv ... | 2003 | 14657126 |
| polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. | in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. | 2004 | 14716262 |
| initial antimicrobial effect of controlled-release doxycycline in subgingival sites. | to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp). | 2004 | 15324352 |
| quantitative detection of periodontopathogens by real-time pcr. | specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. we report here the development of a sensitive, quantitative pcr technique for enumerating actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), dialister pneumosintes (dp) and micromonas micros (mm) as well as total eubacteria in subgingival plaque samples from ... | 2004 | 15325758 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| herpesviruses, the missing link between gingivitis and periodontitis? | herpesviruses appear to assume a major etiopathogenic role in various types of destructive periodontal disease. human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hcmv-ebv co-infection are closely associated with disease-active periodontitis in juveniles and adults, with acute necrotizing ulcerative gingivitis in children, and with periodontal abscesses. in particular, hcmv reactivation in periodontitis lesions seems to be linked to advancing disease. hcmv infects periodontal monocytes/m ... | 2004 | 15553977 |
| simultaneous detection of dialister pneumosintes and filifactor alocis in endodontic infections by 16s rdna-directed multiplex pcr. | dialister pneumosintes and filifactor alocis have been recently considered as candidate endodontic pathogens. in this study, we devised a 16s rdna-directed multiplex pcr protocol for simultaneous detection of these two bacterial species in endodontic infections. samples were taken from infected root canals associated with asymptomatic periradicular lesions as well as from cases of acute periradicular abscesses. dna extracted from the samples was used as template for simultaneous detection of d. ... | 2004 | 15564862 |
| differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. | this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations. | 2005 | 15829891 |
| real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. | accurate laboratory tests for the detection and quantification of periodontopathogens in subgingival plaque samples of periodontal disease patients are becoming essential to study the pathogenesis of this polymicrobial condition. we used a real-time polymerase chain reaction (pcr) assay for the quantification of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, dialister pneumosintes, campylobacter rectus, and micromonas micros as well as total eubacteria in ... | 2005 | 16171445 |
| dialister micraerophilus sp. nov. and dialister propionicifaciens sp. nov., isolated from human clinical samples. | seventeen anaerobic, gram-negative, tiny coccobacilli were collected in france from various human clinical samples. biochemical analyses as well as molecular studies, including 16s rrna and dnak gene sequencing, affiliated all the isolates to the genus dialister. however, 16s rrna and dnak gene sequence similarities were below 95.2 and 79.7 %, respectively, when comparisons were performed with the currently described species dialister pneumosintes and dialister invisus. two clusters consisting o ... | 2005 | 16280512 |
| asaccharolytic anaerobic gram-negative coccobacilli (aagnc) isolated from infected root canals and periodontal pockets. | culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (aagnc), may constitute a predominant group of organisms in oral sites. this study aimed to characterize phylogenetically 10 aagnc isolated from endodontic lesions and periodontal pockets. | 2006 | 16390338 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| periodontal status in smokers and never-smokers: clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens. | smoking is a well-known risk factor for destructive periodontal disease, but its relationship with periodontal status and subgingival microbiota remains unclear. inherent limitations of microbiological methods previously used may partly explain these mixed results, and real-time polymerase chain reaction (pcr) has been presented as a valid alternative. the aim of the present study was to investigate the clinical condition and microbiological profile of patients with chronic periodontitis as rela ... | 2006 | 16945023 |
| isolation of dialister pneumosintes isolated from a bacteremia of vaginal origin. | in this report, we review one case of bacteremia infection due to dialister pneumosintes. the patient was admitted in post-partum with vaginosis and suppurative thrombosis of the ovarian veina. d. pneumosintes was isolated in pure culture from the three blood culture flasks. identification of this bacterium was difficult and requires the amplification and partial sequencing of the 16s rrna gene. the patients had favorable outcome after antibiotic treatment. | 2006 | 16965925 |
| characterization of dialister species in infected root canals. | members of the dialister genus are asaccharolytic obligately anaerobic gram-negative coccobacilli that are culture-difficult or remain uncultivated. their participation in endodontic infections has been only consistently demonstrated after advent of molecular biology approaches. this study was undertaken to characterize dialister species in samples from primary endodontic infections using a devised 16s rrna gene-based group-specific heminested pcr assay followed by sequencing of pcr products. ge ... | 2006 | 17055906 |
| prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. | evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. this study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; portland, or, and rio de janeiro, brazil. dna was extracted from aspirates and used as template in nested ... | 2006 | 17174667 |
| molecular analysis of oral and respiratory bacterial species associated with ventilator-associated pneumonia. | trauma intensive care unit (ticu) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (vap). oral and oropharyngeal bacteria are believed to be responsible for many cases of vap, but definitive evidence of this relationship is lacking. earlier studies used conventional culture-based methods for identification of bacterial pathogens, but these methods are insufficient, as some bacteria may be uncultivable or difficult to grow. the purpose of this s ... | 2007 | 17301280 |
| herpesviral-bacterial synergy in the pathogenesis of human periodontitis. | periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role. | 2007 | 17471038 |
| [subgingival microbiota in chronic and aggressive periodontitis in bogotá, colombia: an epidemiological approach]. | periodontitis-associated microbiotas differ in regions around the world and vary with a variety of factors. this suggests that each country must establish its own dental microbiobiotic profile in order to formulate adequate prevention measures and more specific treatments. | 2007 | 17546221 |
| periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: a case-control study. | recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. this case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (cad) based on clinical and periodontal microbiologic parameters. | 2007 | 17760542 |
| antimicrobial susceptibilities and clinical sources of dialister species. | seventy-four strains representing the four species of the genus dialister were isolated from various clinical samples. dialister pneumosintes and dialister micraerophilus were the two mainly encountered species. fifty-five isolates were tested against 14 antimicrobial agents. decreased susceptibilities to piperacillin, metronidazole, macrolides, fluoroquinolones, and rifampin were demonstrated. the clinical impact of these decreased susceptibilities remains to be investigated but should prompt m ... | 2007 | 17923492 |
| bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. | the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. | 2007 | 17949344 |
| detection of dialister pneumosintes in the subgingival biofilm of subjects with periodontal disease. | dialister pneumosintes has been indicated as a potentially new periodontopathic species. this study evaluated the prevalence of this microorganism in saliva and subgingival biofilm from subjects with different periodontal conditions. subgingival biofilm and saliva samples from 48 subjects with periodontal health (ph) and 116 patients with chronic periodontitis (cp) were obtained. dna was extracted from the samples and the presence of d. pneumosintes was determined by pcr. differences in clinical ... | 2007 | 17980633 |
| occurrence of herpes simplex virus 1 and three periodontal bacteria in patients with chronic periodontitis and necrotic pulp. | viral and bacterial associations appear to be implicated in the development of periodontal infections. little information is available describing the periodontopathic agents in root canals with necrotic pulp. in this study, the occurrence and the combinations among herpes simplex virus type 1 (hsv-1) and dialister pneumosintes, tannerella forsythia, and treponema denticola in patients with chronic periodontitis and necrotic pulp were evaluated. clinical samples from healthy subjects and patients ... | 2008 | 18389006 |
| molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients. | there is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (cf) patients. | 2008 | 18682840 |
| the effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction. | the aim of the present study was to evaluate the effect of strict supragingival plaque control on the subgingival microbiota in smokers and never-smokers. research into the impact of supragingival plaque control on the number of subgingival bacteria has resulted in contradictory findings. real-time polymerase chain reaction (pcr) has been suggested as a valid alternative to current microbiologic methods based on bacteria cultures. | 2008 | 19053920 |
| [pneumonia due to dialister pneumosintes in a patient with chronic obstructive pulmonary disease]. | | 2010 | 19552995 |
| bone microbial contamination influences autogenous grafting in sinus augmentation. | the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation. | 2009 | 19656037 |
| experimental studies of the nasopharyngeal secretions from influenza patients : iv. anaerobic cultivation. | from the filtered nasopharyngeal washings of patients in the first 36 hours of uncomplicated epidemic influenza and rarely in later stages of the disease, we have cultivated a minute bacilloid body, bacterium pneumosintes, 0.15 to 0.3 microns in length, of constant cultural characters and capable of indefinite propagation on artificial media. this organism, not of the nature of ordinary bacteria, was also recovered in pure culture from the unfiltered and filtered lung tissue of rabbits and guine ... | 1921 | 19868531 |
| experimental studies of the nasopharyngeal secretions from influenza patients : v. bacterium pneumosintes and concurrent infections. | during the course of animal experiments with the anaerobic filter-passing organisms cultivated from epidemic influenzal sources, certain pulmonary infections with ordinary bacteria have been observed. the experiments also have shown that the lungs of animals infected with bacterium pneumosintes are less resistant than normal lungs to infection with ordinary bacteria. the demonstration of this fact invites a comparison of the course of these experimental bacterial infections with the sequence of ... | 1921 | 19868538 |
| experimental studies of the nasopharyngeal secretions from influenza patients : vi. immunity reactions. | the experiments described furnish additional evidence of the pathogenic character and the virtual identity of the various strains of the active agent derived from the nasopharyngeal secretions of influenzal patients with which the transmission experiments in rabbits have been carried out. the active material has been shown to be of antigenic nature, so that rabbits are protected from the effects of a second inoculation. the experiments indicate also the antigenic identity of the various strains ... | 1922 | 19868584 |
| experimental studies of the nasopharyngeal secretions from influenza patients : vii. serological reactions. | cultivation of bacterium pneumosintes in the collodion sac dialysate of a tissue medium produces an antigen suitable for serological tests. injection of dialysate cultures of bacterium pneumosintes into rabbits results in the production of antibodies demonstrable by agglutination, precipitation, complement fixation, and phagocytic reactions. four strains of bacterium pneumosintes, three from the first epidemic influenzal wave (1918-19) and one from the second (1920), show identical antigenic cha ... | 1922 | 19868628 |
| experimental studies of the nasopharyngeal secretions from influenza patients : vii. further observations on the cultural and morphological characters of bacterium pneumosintes. | after artificial cultivation for a period of over 3 years bacterium pneumosintes has maintained its original morphological and cultural characteristics, when grown in the original medium. adaptation to a saprophytic existence has been accompanied by a loss of pathogenicity. our strains now grow readily under strictly anaerobic conditions in a variety of media with peptone broth as a base, enriched with fresh tissue, blood, or by the growth of other bacteria. surface colonies have been obtained o ... | 1922 | 19868647 |
| experimental studies of the nasopharyngeal secretions from influenza patients : ix. the recurrence of 1922. | from the nasopharyngeal secretions of patients in the early hours of uncomplicated epidemic influenza during the recurrence in new york city in january and february, 1922, we have again obtained an active agent, pathogenic for rabbits, and have identified this active agent as bacterium pneumosintes. four new strains of this micro-organism have been isolated in pure culture and identified with the 1918-19 and 1920 strains on morphological, cultural, and serological grounds. all of the significant ... | 1922 | 19868690 |
| experimental studies of the nasopharyngeal secretions from influenza patients : x. the immunizing effects in rabbits of subcutaneous injections of killed cultures of bacterium pneumosintes. | a series of rabbits was subcutaneously injected with three measured doses of killed cultures of two strains of bacterium pneumosintes derived from the nasopharyngeal secretions of influenza patients. these rabbits were subsequently tested for the development of serum antibodies and for the presence of an induced immunity to the living organisms, with the following results. the serum of eleven of fifteen rabbits, tested from 10 to 27 days after the final subcutaneous injection, specifically agglu ... | 1922 | 19868702 |
| experimental studies of the nasopharyngeal secretions from influenza patients : xi. antibodies in the blood after recovery from epidemic influenza. | although bacterium pneumosintes is a stable organism which agglutinates only in low dilutions of the serum of actively immunized rabbits, the serological reactions of this microorganism have always been found to be consistent and specific. the results of the agglutination and precipitation reactions described in this paper indicate that the serum of normal persons does not contain demonstrable agglutinins or precipitins for bacterium pneumosintes. by contrast, agglutinins have been demonstrated ... | 1923 | 19868727 |
| the cultivation of anaerobic treponemata on the surface of blood agar plates. | by a method of cultivation that proved successful with bacterium pneumosintes, characteristic surface colonies of old saprophytic strains of treponema pallidum and treponema calligyrum have been obtained. the presence of two types of colony in the pallidum cultures requires explanation, but the fact of colony formation may point the way to new methods of isolation and identification and to the utilization of suspensions of treponemata, free from foreign proteins, for serological and immunologica ... | 1923 | 19868728 |
| experimental studies of the nasopharyngeal secretions from influenza patients : xii. the effects of subcutaneous injections of vaccines of bacterium pneumosintes in man. | thirteen volunteers were injected subcutaneously with a vaccine prepared from two strains of bacterium pneumosintes. three doses were given corresponding, by opacity tests, to 1,000, 2,000, and 2,000 million staphylococci. the intervals between the several injections were 5 and 8 days. local reactions of mild and transitory character only were noted. constitutional reactions, also mild and transitory, consisting of headache, depression, and generalized muscular pains, were infrequently observed. ... | 1923 | 19868739 |
| studies on the nasopharyngeal secretions from patients with common colds. | from the foregoing experiments it appears that with the filtered nasopharyngeal secretions from early cases of typical infections common colds in the first 3 to 18 hours of the disease, a similar condition can be transmitted to man. with the unheated but not with the heated secretions from four of six such patients we have succeeded in transmitting an affection indistinguishable from common cold to four men and in two instances the condition was conveyed from the person with the experimental dis ... | 1923 | 19868800 |
| an attempt to isolate a filter-passing virus in epidemic influenza. | 1. six cases of epidemic influenza in various stages of disease were investigated. 2. suspicious cultures of bodies resembling bacterium pneumosintes were obtained in three cases, twice from the lung filtrates of inoculated animals and once from the filtrate of nasopharyngeal washings direct. 3. in these instances material was obtained within 36 hours of the acute onset, thus confirming the observations of olitsky and gates. 4. macroscopic and microscopic findings in the lungs of inoculated anim ... | 1924 | 19868833 |
| the relation of bacterium pneumosintes to influenza: a study with a strain of the organism derived from the nasopharyngeal washings of a case of influenza. | nasopharyngeal washings from a case of epidemic influenza have proven capable of initiating a pathological change in rabbits and in guinea pigs characterized after an incubation period of 1 or 2 days, by some elevation of temperature, reduction in the number of circulating leucocytes, especially of the mononuclears, and by a pulmonary lesion during the period of reaction, which is distinguishable from those accidentally incurred at the time of death. from one such animal, in the second passage o ... | 1926 | 19869205 |
| bacterium pneumosintes in clinical influenza in new york city in 1926. | the presence of bacterium pneumosintes has been demonstrated in nasopharyngeal washings from 2 patients in a sporadic outbreak of clinical influenza in new york city in march, 1926. 2 strains of bacteria morphologically similar to bact. pneumosintes, but differing in certain cultural characters, and 2 other anaerobic filter-passing organisms were also isolated from the 9 patients examined. the blood serum of 16 among 17 persons convalescent from clinical influenza, and of 6 among 10 supposedly n ... | 1926 | 19869223 |
| prevalence of potential bacterial respiratory pathogens in the oral cavity of hospitalised individuals. | to assess the prevalence of oral colonisation by bacterial respiratory pathogens in hospitalised patients. | 2010 | 19939349 |
| researches on bacterium pneumosintes (olitsky and gates). improved methods of cultivation, with special reference to vaccine production. | | 1925 | 19984568 |
| bacterium pneumosintes and influenza. | | 1927 | 20316132 |
| response of subgingival bacteria to smoking cessation. | it has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. the aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. subgingival plaque was collected from 22 smokers at the baseline and 12 month ... | 2010 | 20410352 |
| subgingival microbial profiles of smokers with periodontitis. | the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ... | 2010 | 20739702 |
| bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion. | due to the environmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. however, little is known about the microbial risk present in sludge and its stabilized products. this study microbiologically explored the effects of anaerobic lagoon fermentation (alf) and autothermal thermophilic aerobic digestion (atad) on pathogen-related risk of raw swine manure by using culture-independent 16s rdna cloning and ... | 2010 | 20922382 |
| impact of systemic antimicrobials combined with anti-infective mechanical debridement on the microbiota of generalized aggressive periodontitis: a 6-month rct. | to compare the effects of systemic amoxicillin (amx) plus metronidazole (met) or placebos combined with anti-infective mechanical debridement on the sub-gingival microbiota of generalized aggressive periodontitis (gap). | 2011 | 21303403 |
| viral-bacterial associations in acute apical abscesses. | objective: viral-bacterial and bacterial synergism have been suggested to contribute to the pathogenesis of several human diseases. this study sought to investigate the possible associations between 9 candidate endodontic bacterial pathogens and 9 human viruses in samples from acute apical abscesses. study design: dna extracts from purulent exudate aspirates of 33 cases of acute apical abscess were surveyed for the presence of 9 selected bacterial species using a 16s ribosomal rna gene-based nes ... | 2011 | 21507684 |
| clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole. | background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ... | 2011 | 22060046 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |
| microbiologic profile of endodontic infections from hiv- and hiv+ patients using multiple-displacement amplification and checkerboard dna-dna hybridization. | to compare the microbiota of endodontic infections in necrotic pulp from hiv-negative and hiv-positive subjects. | 2012 | 22335194 |
| oral bacterial dna findings in pericardial fluid. | we recently reported that large amounts of oral bacterial dna can be found in thrombus aspirates of myocardial infarction patients. some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing and a few with pcr; in purulent pericarditis, nevertheless, bacterial pcr has not been used as a diagnostic method before. | 2014 | 25412607 |
| exploring salivary microbiota in aids patients with different periodontal statuses using 454 gs-flx titanium pyrosequencing. | patients with acquired immunodeficiency syndrome (aids) are at high risk of opportunistic infections. oral manifestations have been associated with the level of immunosuppression, these include periodontal diseases, and understanding the microbial populations in the oral cavity is crucial for clinical management. the aim of this study was to examine the salivary bacterial diversity in patients newly admitted to the aids ward of the public health clinical center (china). saliva samples were colle ... | 2015 | 26191508 |
| periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased dominican adults. | the objective of this study was to compare the periodontopathogen prevalence and tetracycline resistance genes in dominican patients with different periodontal conditions. | 2015 | 26121972 |
| suppuration-associated bacteria in patients with chronic and aggressive periodontitis. | suppuration (sup) on probing may be an indication of active periodontal breakdown. the aim of the present study is to analyze which subgingival species are associated with sup in patients with chronic (cp) and aggressive (agp) periodontitis. | 2013 | 23327648 |
| the association between detectable plasmatic human immunodeficiency virus (hiv) viral load and different subgingival microorganisms in brazilian adults with hiv: a multilevel analysis. | this study investigates the association between detectable plasmatic human immunodeficiency virus (hiv) viral load (hvl) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with hiv. | 2014 | 23952074 |
| subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions. | this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ... | 2012 | 22710412 |
| microbial ecosystem analysis in root canal infections refractory to endodontic treatment. | the purpose of this study was to combine multiple displacement amplification and checkerboard dna-dna hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment. | 2016 | 27377440 |
| microbial diversity in persistent root canal infections investigated by checkerboard dna-dna hybridization. | the aim of the present study was to investigate the composition of the root canal microbiota in endodontic failures in order to identify and quantify these microorganisms. | 2014 | 24935532 |
| association of three putative periodontal pathogens with chronic periodontitis in brazilian subjects. | objective the aim of this study was to evaluate the association of porphyromonas endodontalis, filifactor alocis and dialister pneumosintes with the occurrence of periodontitis. material and methods thirty subjects with chronic periodontitis (chp) and 10 with periodontal health (ph) were included in the study. nine subgingival biofilm samples were collected as follows: i) ph group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) chp group - from t ... | 2016 | 27119767 |
| a case of bacteremia caused by dialister pneumosintes and slackia exigua in a patient with periapical abscess. | dialister pneumosintes and slackia exigua are both obligatory anaerobe and known to be associated with periodontal diseases and other oral infection. we report a case of blood stream infection caused by d. pneumosintes and s. exigua. this occurred in a 78-year-old female patient that presented with general weakness and fever. we revealed that she had a periapical abscess. the blood culture was positive for d. pneumosintes and s. exigua; however, identifying them was challenging. ultimately, 16s ... | 2016 | 26612007 |
| dialister pneumosintes bacteremia caused by dental caries and sinusitis. | a 62-year-old japanese woman was hospitalized at the department of senology for positive signals on two sets of blood cultures obtained in the emergency department. the initial physical examination with enhanced computed tomography of the chest and abdomen did not identify the infectious source. dialister pneumosintes was identified on 16s rrna sequencing, and dental caries with sinusitis were subsequently diagnosed based on a dental examination and magnetic resonance imaging. history taking wit ... | 2015 | 25786460 |
| macrophage cell activation with acute apical abscess contents determined by interleukin-1 beta and tumor necrosis factor alpha production. | this clinical study has investigated the antigenic activity of bacterial contents from exudates of acute apical abscesses (aaas) and their paired root canal contents regarding the stimulation capacity by levels of interleukin (il)-1 beta and tumor necrosis factor alpha (tnf-α) throughout the root canal treatment against macrophage cells. | 2014 | 25205261 |
| a case of coinfection in a chronic maxillary sinusitis of odontogenic origin: identification of dialister pneumosintes. | in this report, we discuss the case of a 39-year-old woman presenting with a case of chronic maxillary sinusitis. | 2013 | 23880283 |
| molecular methods for diagnosis of odontogenic infections. | historically, the identification of microorganisms has been limited to species that could be cultured in the microbiology laboratory. the purpose of the present study was to apply molecular techniques to identify microorganisms in orofacial odontogenic infections (ois). | 2012 | 22326175 |