| phylogenetic placement of dialister pneumosintes (formerly bacteroides pneumosintes) within the sporomusa subbranch of the clostridium subphylum of the gram-positive bacteria. | the nucleotide sequence of the 16s rrna gene of the type strain of dialister pneumosintes was determined. phylogenetic analysis revealed that this species belongs to the sporomusa branch of the clostridium subphylum of the gram-positive bacteria and should therefore be excluded from the family bacteroidaceae. within this branch, which encompasses several other gram-negative taxa, such as acidaminococcus, pectinatus, phascolarcobacterium, quinella, selenomonas, and zymophilus, dialister showed a ... | 1995 | 7537075 |
| oribaculum catoniae gen. nov., sp. nov.; catonella morbi gen. nov., sp. nov.; hallella seregens gen. nov., sp. nov.; johnsonella ignava gen. nov., sp. nov.; and dialister pneumosintes gen. nov., comb. nov., nom. rev., anaerobic gram-negative bacilli from the human gingival crevice. | the following four new species of anaerobic gram-negative bacilli isolated from the human gingival crevice are described: oribaculum catoniae, with atcc 51270 as the type strain; catonella morbi, with atcc 51271 as the type strain; hallella seregens, with atcc 51272 as the type strain; and johnsonella ignava, with atcc 51276 as the type strain. c. morbi is associated with periodontitis. h. seregens and j. ignava are associated with gingivitis and periodontitis. o. catoniae has been isolated from ... | 1994 | 8186083 |
| molecular identification of dialister pneumosintes in subgingival plaque of humans. | dialister pneumosintes is a nonfermentative, anaerobic, gram-negative rod that grows with small, circular, transparent, shiny, smooth colonies on blood agar. even though d. pneumosintes has been recovered from deep periodontal pockets, little is known about the relationship between the organism and destructive periodontal disease. this study describes a rapid pcr method to identify d. pneumosintes in periodontal samples. the pcr identification method detected as little as 10 pg of d. pneumosinte ... | 2000 | 10921975 |
| importance of dialister pneumosintes in human periodontitis. | anaerobic bacteria play important roles in the pathogenesis of human periodontitis. this study examined the relationship between a potentially new periodontopathic bacterium dialister pneumosintes and periodontal disease. a total of 73 women and 62 men aged 18 to 86 years participated in the study. using a 16s rrna polymerase chain reaction identification method, the presence of d. pneumosintes was determined in paper-point samples from periodontal pockets of 105 periodontitis and 30 gingivitis ... | 2000 | 11154414 |
| herpes viruses and periodontopathic bacteria in early-onset periodontitis. | this study examined the occurrence of human herpes viruses and suspected periodontopathic bacteria in early-onset periodontitis patients who experienced progressive disease in at least 2 periodontal sites during the maintenance phase of therapy. | 2001 | 11493359 |
| molecular identification of microorganisms from endodontic infections. | a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ... | 2001 | 11526164 |
| characterisation of eubacterium-like strains isolated from oral infections. | the genus eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. as a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. in this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the ... | 2001 | 11699590 |
| dialister pneumosintes, a new putative periodontal pathogen. | recent studies have implicated dialister pneumosintes as a candidate periodontal pathogen. this study determined the association of subgingival d. pneumosintes with demographic variables (age and gender) and the presence of subgingival periodontopathic bacteroides forsythus and porphyromonas gingivalis. microbial identification by established pcr techniques was performed in samples from 149 periodontitis patients. subgingival d. pneumosintes occurred with significantly higher prevalence in older ... | 2002 | 11842941 |
| dialister pneumosintes associated with human brain abscesses. | in this report, we review two cases of brain infection due to dialister pneumosintes in previously healthy patients. the bacterium was isolated from the first patient by blood culture and directly from a brain abscess in the second patient. in both cases, the infection was suspected to be of nasopharyngeal or dental origin. the patients had favorable outcomes following surgical debridement and antibiotic treatment. after in vitro amplification and partial sequencing of the 16s rrna gene, two str ... | 2002 | 12354905 |
| dialister pneumosintes can be a suspected endodontic pathogen. | dialister pneumosintes is a nonmotile, nonfermentative, non-spore-forming, obligately anaerobic, gram-negative bacillus that has been associated with some infections in the human body. a species-specific nested polymerase chain reaction assay was used to investigate the occurrence of d pneumosintes in primary root canal infections. | 2002 | 12374926 |
| molecular and cultural analysis of the microflora associated with endodontic infections. | cultural studies have indicated that a subset of the oral microflora is responsible for endodontic infections. approximately 50% of oral bacteria are unculturable, so it is likely that currently unknown bacteria are present in such infections. in this study, cultural and molecular analyses were performed on the microflora in aspirate samples collected from 5 infected root canals. 16s rdna sequences from 261 isolates and 624 clones were identified by comparison with database sequences. sixty-five ... | 2002 | 12407091 |
| cytomegalovirus periodontal presence is associated with subgingival dialister pneumosintes and alveolar bone loss. | destructive periodontal disease is associated with human cytomegalovirus (hcmv), epstein-barr type 1 virus (ebv-1) and other members of the herpesviridae family as well as with various gram-negative anaerobic bacteria, including the dialister pneumosintes species. this study aimed to determine possible interrelationships between periodontal hcmv, ebv-1, herpes simplex virus and d. pneumosintes, and relate the microbiological findings to periodontitis clinical status. sixteen subjects each contri ... | 2002 | 12485328 |
| diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients. | the primary purpose of the present study was to compare the microbial profiles of the tongue dorsa of healthy subjects and subjects with halitosis by using culture-independent molecular methods. our overall goal was to determine the bacterial diversity on the surface of the tongue dorsum as part of our ongoing efforts to identify all cultivable and not-yet-cultivated species of the oral cavity. tongue dorsum scrapings were analyzed from healthy subjects with no complaints of halitosis and subjec ... | 2003 | 12574246 |
| allisonella histaminiformans gen. nov., sp. nov. a novel bacterium that produces histamine, utilizes histidine as its sole energy source, and could play a role in bovine and equine laminitis. | when cattle and horses are fed large amounts of grain, histamine can accumulate in the gastrointestinal tract, and this accumulation can cause an acute inflammation of the hooves (laminitis). when ruminal fluid from dairy cattle fed grain supplements was serially diluted in anaerobic mrs medium containing histidine (50 mm), histamine was detected at dilutions as high as 10(-7). the histidine enrichments were then transferred successively in an anaerobic, carbonate-based medium (50 mm histidine) ... | 2002 | 12583709 |
| herpesviral-bacterial interactions in aggressive periodontitis. | human cytomegalovirus, epstein-barr virus and herpesvirus co-infections occur with significantly higher frequency in actively progressing than in stable periodontitis sites of adolescents and young adults. also, periodontal presence of cytomegalovirus and epstein-barr virus is associated with increased occurrence of subgingival porphyromonas gingivalis, bacteroides forsythus, dialister pneumosintes, prevotella intermedia, prevotella nigrescens, treponema denticola and actinobacillus actinomycete ... | 2003 | 12716334 |
| pcr methodology as a valuable tool for identification of endodontic pathogens. | this paper reviews the principles of polymerase chain reaction (pcr) methodology, its application in identification of endodontic pathogens and the perspectives regarding the knowledge to be reached with the use of this highly sensitive, specific and accurate methodology as a microbial identification test. | 2003 | 12799118 |
| positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections. | dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ... | 2003 | 12877258 |
| [mimeae and short forms of bacteria]. | | 1953 | 13124989 |
| mima polymorpha meningitis; report of a case and review of the literature. | | 1958 | 13517502 |
| the chemical composition of dialister pneumosintes and the significance of the studies of small eubacteria. | | 1962 | 14020486 |
| dialister invisus sp. nov., isolated from the human oral cavity. | six strains of anaerobic, gram-negative coccobacilli isolated from the root canals of patients with endodontic infections (five strains) and from a deep periodontal pocket (one strain) were subjected to a comprehensive range of phenotypic and genetic tests and were found to comprise a homogeneous group. following 16s rrna gene sequence analysis, they were found to be most closely related to dialister pneumosintes, with 93 % sequence similarity between the two taxa. a novel species, dialister inv ... | 2003 | 14657126 |
| culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. | renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ... | 2003 | 14662931 |
| polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. | in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. | 2004 | 14716262 |
| initial antimicrobial effect of controlled-release doxycycline in subgingival sites. | to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp). | 2004 | 15324352 |
| quantitative detection of periodontopathogens by real-time pcr. | specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. we report here the development of a sensitive, quantitative pcr technique for enumerating actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), dialister pneumosintes (dp) and micromonas micros (mm) as well as total eubacteria in subgingival plaque samples from ... | 2004 | 15325758 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| herpesviruses, the missing link between gingivitis and periodontitis? | herpesviruses appear to assume a major etiopathogenic role in various types of destructive periodontal disease. human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hcmv-ebv co-infection are closely associated with disease-active periodontitis in juveniles and adults, with acute necrotizing ulcerative gingivitis in children, and with periodontal abscesses. in particular, hcmv reactivation in periodontitis lesions seems to be linked to advancing disease. hcmv infects periodontal monocytes/m ... | 2004 | 15553977 |
| simultaneous detection of dialister pneumosintes and filifactor alocis in endodontic infections by 16s rdna-directed multiplex pcr. | dialister pneumosintes and filifactor alocis have been recently considered as candidate endodontic pathogens. in this study, we devised a 16s rdna-directed multiplex pcr protocol for simultaneous detection of these two bacterial species in endodontic infections. samples were taken from infected root canals associated with asymptomatic periradicular lesions as well as from cases of acute periradicular abscesses. dna extracted from the samples was used as template for simultaneous detection of d. ... | 2004 | 15564862 |
| molecular analysis of microbial diversity in advanced caries. | real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ... | 2005 | 15695690 |
| differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. | this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations. | 2005 | 15829891 |
| uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. | endodontic infections have been traditionally studied by culture methods, but recent reports showing that over 50% of the oral microbiota is still uncultivable (b. j. paster et al., j. bacteriol. 183:3770-3783, 2001) raise the possibility that many endodontic pathogens remain unknown. this study intended to investigate the prevalence of several uncultivated oral phylotypes, as well as newly named species in primary or persistent endodontic infections associated with chronic periradicular disease ... | 2005 | 16000454 |
| bacterial diversity in necrotizing ulcerative periodontitis in hiv-positive subjects. | necrotizing ulcerative periodontitis (nup) is a painful and potentially debilitating affliction that affects about 2% to 6% of hiv-positive subjects. nup may be caused by specific microorganisms that are presently unknown or by microbial species not usually thought to cause periodontal infections. the purpose of this study was to define the bacterial species associated with nup in hiv-positive patients. | 2002 | 16013212 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| biochemical differentiation and comparison of desulfovibrio species and other phenotypically similar genera. | seventeen human clinical isolates representing four species of desulfovibrio were characterized using 16s rrna gene sequences and tests for catalase, indole, nitrate, bile, urease, formate-fumarate stimulation, desulfoviridin, motility, and hydrogen sulfide production, plus susceptibility to antimicrobial agents. eighty additional strains representing 10 phenotypically similar genera (bilophila, selenomonas, capnocytophaga, campylobacter, bacteroides, sutterella, anaerobiospirillum, dialister, v ... | 2005 | 16081948 |
| detection of novel oral species and phylotypes in symptomatic endodontic infections including abscesses. | this study was undertaken to investigate the occurrence of several uncultivated phylotypes and newly named bacterial species in symptomatic endodontic infections. samples taken from cases clinically diagnosed as acute periradicular abscesses or acute periradicular periodontitis were surveyed for the presence of 12 taxa by means of a 16s rrna-gene-based nested or hemi-nested pcr assay. the most prevalent of the target taxa were dialister invisus, olsenella uli, granulicatella adiacens, and synerg ... | 2005 | 16099112 |
| real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. | accurate laboratory tests for the detection and quantification of periodontopathogens in subgingival plaque samples of periodontal disease patients are becoming essential to study the pathogenesis of this polymicrobial condition. we used a real-time polymerase chain reaction (pcr) assay for the quantification of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, dialister pneumosintes, campylobacter rectus, and micromonas micros as well as total eubacteria in ... | 2005 | 16171445 |
| molecular methodology in determining vaginal flora in health and disease: its time has come. | the microbial flora of the human vagina can affect the health of women, their fetuses, and newborns. conventional cultivation methods fail to detect some fastidious vaginal bacteria, leading to an incomplete census. recent advances in molecular biology have facilitated the detection and identification of bacteria without cultivation, and the advantages and limitations of this approach are described. molecular studies of the vaginal flora have discovered many uncultivated bacterial species. for i ... | 2005 | 16225785 |
| dialister micraerophilus sp. nov. and dialister propionicifaciens sp. nov., isolated from human clinical samples. | seventeen anaerobic, gram-negative, tiny coccobacilli were collected in france from various human clinical samples. biochemical analyses as well as molecular studies, including 16s rrna and dnak gene sequencing, affiliated all the isolates to the genus dialister. however, 16s rrna and dnak gene sequence similarities were below 95.2 and 79.7 %, respectively, when comparisons were performed with the currently described species dialister pneumosintes and dialister invisus. two clusters consisting o ... | 2005 | 16280512 |
| asaccharolytic anaerobic gram-negative coccobacilli (aagnc) isolated from infected root canals and periodontal pockets. | culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (aagnc), may constitute a predominant group of organisms in oral sites. this study aimed to characterize phylogenetically 10 aagnc isolated from endodontic lesions and periodontal pockets. | 2006 | 16390338 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| periodontal status in smokers and never-smokers: clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens. | smoking is a well-known risk factor for destructive periodontal disease, but its relationship with periodontal status and subgingival microbiota remains unclear. inherent limitations of microbiological methods previously used may partly explain these mixed results, and real-time polymerase chain reaction (pcr) has been presented as a valid alternative. the aim of the present study was to investigate the clinical condition and microbiological profile of patients with chronic periodontitis as rela ... | 2006 | 16945023 |
| isolation of dialister pneumosintes isolated from a bacteremia of vaginal origin. | in this report, we review one case of bacteremia infection due to dialister pneumosintes. the patient was admitted in post-partum with vaginosis and suppurative thrombosis of the ovarian veina. d. pneumosintes was isolated in pure culture from the three blood culture flasks. identification of this bacterium was difficult and requires the amplification and partial sequencing of the 16s rrna gene. the patients had favorable outcome after antibiotic treatment. | 2006 | 16965925 |
| characterization of dialister species in infected root canals. | members of the dialister genus are asaccharolytic obligately anaerobic gram-negative coccobacilli that are culture-difficult or remain uncultivated. their participation in endodontic infections has been only consistently demonstrated after advent of molecular biology approaches. this study was undertaken to characterize dialister species in samples from primary endodontic infections using a devised 16s rrna gene-based group-specific heminested pcr assay followed by sequencing of pcr products. ge ... | 2006 | 17055906 |
| on the use of denaturing gradient gel electrophoresis approach for bacterial identification in endodontic infections. | bacteria in infected root canals of teeth evincing chronic apical periodontitis lesions were identified by a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) approach. dna was extracted from root canal samples, and part of the 16s rrna gene of all bacteria was amplified by pcr and separated by dgge, generating banding patterns representative of the community structure. twenty visible bands were cut out of the gel, re-amplified, and sequenced to provide identification. ... | 2007 | 17115203 |
| prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. | evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. this study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; portland, or, and rio de janeiro, brazil. dna was extracted from aspirates and used as template in nested ... | 2006 | 17174667 |
| molecular analysis of oral and respiratory bacterial species associated with ventilator-associated pneumonia. | trauma intensive care unit (ticu) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (vap). oral and oropharyngeal bacteria are believed to be responsible for many cases of vap, but definitive evidence of this relationship is lacking. earlier studies used conventional culture-based methods for identification of bacterial pathogens, but these methods are insufficient, as some bacteria may be uncultivable or difficult to grow. the purpose of this s ... | 2007 | 17301280 |
| subgingival plaque microbiota in hiv positive patients. | to describe and compare the predominant bacterial and fungal species associated with gingivitis, periodontitis, and linear gingival erythema (lge), in hiv positive subjects with different immune status. | 2007 | 17309593 |
| herpesviral-bacterial synergy in the pathogenesis of human periodontitis. | periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role. | 2007 | 17471038 |
| [subgingival microbiota in chronic and aggressive periodontitis in bogotá, colombia: an epidemiological approach]. | periodontitis-associated microbiotas differ in regions around the world and vary with a variety of factors. this suggests that each country must establish its own dental microbiobiotic profile in order to formulate adequate prevention measures and more specific treatments. | 2007 | 17546221 |
| periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: a case-control study. | recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. this case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (cad) based on clinical and periodontal microbiologic parameters. | 2007 | 17760542 |
| antimicrobial susceptibilities and clinical sources of dialister species. | seventy-four strains representing the four species of the genus dialister were isolated from various clinical samples. dialister pneumosintes and dialister micraerophilus were the two mainly encountered species. fifty-five isolates were tested against 14 antimicrobial agents. decreased susceptibilities to piperacillin, metronidazole, macrolides, fluoroquinolones, and rifampin were demonstrated. the clinical impact of these decreased susceptibilities remains to be investigated but should prompt m ... | 2007 | 17923492 |
| bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. | the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. | 2007 | 17949344 |
| detection of dialister pneumosintes in the subgingival biofilm of subjects with periodontal disease. | dialister pneumosintes has been indicated as a potentially new periodontopathic species. this study evaluated the prevalence of this microorganism in saliva and subgingival biofilm from subjects with different periodontal conditions. subgingival biofilm and saliva samples from 48 subjects with periodontal health (ph) and 116 patients with chronic periodontitis (cp) were obtained. dna was extracted from the samples and the presence of d. pneumosintes was determined by pcr. differences in clinical ... | 2007 | 17980633 |
| occurrence of herpes simplex virus 1 and three periodontal bacteria in patients with chronic periodontitis and necrotic pulp. | viral and bacterial associations appear to be implicated in the development of periodontal infections. little information is available describing the periodontopathic agents in root canals with necrotic pulp. in this study, the occurrence and the combinations among herpes simplex virus type 1 (hsv-1) and dialister pneumosintes, tannerella forsythia, and treponema denticola in patients with chronic periodontitis and necrotic pulp were evaluated. clinical samples from healthy subjects and patients ... | 2008 | 18389006 |
| microorganisms in root canal-treated teeth from a german population. | posttreatment apical periodontitis is usually associated with persistent or secondary intraradicular infection. this study evaluated the presence and relative levels of 28 bacterial taxa in treated root canals of teeth with posttreatment apical periodontitis from german patients using 16s ribosomal rna (rrna) gene probes in a reverse-capture checkerboard hybridization assay. species-specific polymerase chain reaction (pcr) was also performed to detect enterococcus faecalis and candida albicans. ... | 2008 | 18634922 |
| molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients. | there is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (cf) patients. | 2008 | 18682840 |
| the effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction. | the aim of the present study was to evaluate the effect of strict supragingival plaque control on the subgingival microbiota in smokers and never-smokers. research into the impact of supragingival plaque control on the number of subgingival bacteria has resulted in contradictory findings. real-time polymerase chain reaction (pcr) has been suggested as a valid alternative to current microbiologic methods based on bacteria cultures. | 2008 | 19053920 |
| microbiological findings in subjects with asymptomatic oral lichen planus: a cross-sectional comparative study. | the bacterial colonization of the oral mucosa was evaluated in patients with asymptomatic oral lichen planus (olp) and compared to the microbiologic status in mucosally healthy subjects. | 2008 | 19053926 |
| dialister succinatiphilus sp. nov. and barnesiella intestinihominis sp. nov., isolated from human faeces. | two anaerobic, non-spore-forming, bacteria (yit 11850(t) and yit 11860(t)) that stained gram-negative, were isolated from human faeces. cells of strain yit 11850(t) were coccobacilli, asaccharolytic and largely unreactive, with only traces of lactate and propionate as metabolic end products; however, strain yit 11850(t) was able to decarboxylate succinate to propionate. the dna g+c content of strain yit 11850(t) was 51.9 mol%. following 16s rrna gene sequence analysis, this strain was found to b ... | 2008 | 19060046 |
| the microbiota of acute apical abscesses. | as the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ... | 2009 | 19131319 |
| new bacterial compositions in root-filled teeth with periradicular lesions. | the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ... | 2009 | 19166766 |
| bacteria in the apical root canal of teeth with primary apical periodontitis. | bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis. | 2009 | 19426923 |
| [pneumonia due to dialister pneumosintes in a patient with chronic obstructive pulmonary disease]. | | 2010 | 19552995 |
| molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile. | the microbial etiology of periradicular lesions is recognized; however, the bacterial profile of these lesions is not well elucidated. the purpose of this study was to determine the frequency of bacterial colonization in these lesions and to characterize the bacterial community present in root ends and periradicular lesions. | 2009 | 19567313 |
| bone microbial contamination influences autogenous grafting in sinus augmentation. | the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation. | 2009 | 19656037 |
| negativicoccus succinicivorans gen. nov., sp. nov., isolated from human clinical samples, emended description of the family veillonellaceae and description of negativicutes classis nov., selenomonadales ord. nov. and acidaminococcaceae fam. nov. in the bacterial phylum firmicutes. | three strains of a hitherto unknown, gram-negative, tiny, anaerobic coccus were collected from human clinical samples originating from skin and soft tissues. the three isolates displayed at least 99.9 % identity in their 16s rrna gene sequences and more than 99.8 % identity in their dnak gene sequences. the isolates were affiliated to the family veillonellaceae, the coccobacillus dialister micraerophilus being the most closely related species, but there was no more than 91.1 % identity in the 16 ... | 2010 | 19667386 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| prevalence of potential bacterial respiratory pathogens in the oral cavity of hospitalised individuals. | to assess the prevalence of oral colonisation by bacterial respiratory pathogens in hospitalised patients. | 2010 | 19939349 |
| identification of rhesus macaque genital microbiota by 16s pyrosequencing shows similarities to human bacterial vaginosis: implications for use as an animal model for hiv vaginal infection. | the composition of the lower genital tract microbiota in women is believed to affect the risk of sexually acquiring hiv. since macaque genital microbiota could similarly impact vaginal infection with siv we identified microbiota in 11 rhesus macaques using multitag pyrosequencing of the 16s rrna gene. the microbiota was polymicrobial with a median of nine distinct bacterial taxa per macaque (range 3-16 taxa, each constituting 1% or more of the sequences). taxa frequently found included peptoniph ... | 2010 | 20156101 |
| response of subgingival bacteria to smoking cessation. | it has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. the aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. subgingival plaque was collected from 22 smokers at the baseline and 12 month ... | 2010 | 20410352 |
| subgingival microbial profiles of smokers with periodontitis. | the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ... | 2010 | 20739702 |
| bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion. | due to the environmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. however, little is known about the microbial risk present in sludge and its stabilized products. this study microbiologically explored the effects of anaerobic lagoon fermentation (alf) and autothermal thermophilic aerobic digestion (atad) on pathogen-related risk of raw swine manure by using culture-independent 16s rdna cloning and ... | 2010 | 20922382 |
| specified species in gingival crevicular fluid predict bacterial diversity. | analysis of gingival crevicular fluid (gcf) samples may give information of unattached (planktonic) subgingival bacteria. our study represents the first one targeting the identity of bacteria in gcf. | 2010 | 21049043 |
| dysbiosis of the faecal microbiota in patients with crohn's disease and their unaffected relatives. | a general dysbiosis of the intestinal microbiota has been established in patients with crohn's disease (cd), but a systematic characterisation of this dysbiosis is lacking. therefore the composition of the predominant faecal microbiota of patients with cd was studied in comparison with the predominant composition in unaffected controls. whether dysbiosis is present in relatives of patients cd was also examined. | 2011 | 21209126 |
| comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. | the purpose of this clinical study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl) and 0.12% chlorhexidine digluconate (chx) when used as irrigants during treatment of teeth with apical periodontitis. | 2011 | 21238793 |
| microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis. | the objective was to examine the use of a tailor-made dna microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis. | 2011 | 21272848 |
| impact of systemic antimicrobials combined with anti-infective mechanical debridement on the microbiota of generalized aggressive periodontitis: a 6-month rct. | to compare the effects of systemic amoxicillin (amx) plus metronidazole (met) or placebos combined with anti-infective mechanical debridement on the sub-gingival microbiota of generalized aggressive periodontitis (gap). | 2011 | 21303403 |
| use of 16s ribosomal rna gene analyses to characterize the bacterial signature associated with poor oral health in west virginia. | west virginia has the worst oral health in the united states, but the reasons for this are unclear. this pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease. | 2011 | 21362199 |
| viral-bacterial associations in acute apical abscesses. | objective: viral-bacterial and bacterial synergism have been suggested to contribute to the pathogenesis of several human diseases. this study sought to investigate the possible associations between 9 candidate endodontic bacterial pathogens and 9 human viruses in samples from acute apical abscesses. study design: dna extracts from purulent exudate aspirates of 33 cases of acute apical abscess were surveyed for the presence of 9 selected bacterial species using a 16s ribosomal rna gene-based nes ... | 2011 | 21507684 |
| exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16s rrna. | the characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. the purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16s ribosomal-rna (rrna) sequence analysis. | 2011 | 21689545 |
| The complex vaginal flora of West African women with bacterial vaginosis. | The spectrum of bacteria associated with bacterial vaginosis (BV) has recently expanded through taxonomic changes and the use of molecular methods. These methods have yet to be used in large-scale epidemiological studies in Africa where BV is highly prevalent. | 2011 | 21949860 |
| microbial community composition and reactor performance during hydrogen production in a uasb reactor fed with raw cheese whey inoculated with compost. | this study investigated the microbial community developed in a uasb reactor for hydrogen production and correlated it to reactor performance. the reactor was inoculated with kitchen waste compost and fed with raw cheese whey at two organic loading rates, 20 gcod/ld and 30 gcod/ld. hydrogen production was very variable, using an olr of 30 gcod/ld averaged 1.0 lh(2)/ld with no methane produced under these conditions. the hydrogen yield was also very variable and far from the theoretical. this low ... | 2011 | 22156132 |
| analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients. | this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients. | 2011 | 21846535 |
| clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole. | background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ... | 2011 | 22060046 |
| Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing. | It is important to know the comprehensive microbial communities of fecal pollution sources and receiving water bodies for microbial source tracking. Pyrosequencing targeting the V1-V3 hypervariable regions of the 16S rRNA gene was used to investigate the characteristics of bacterial and Bacteroidales communities in major fecal sources and river waters. Diversity analysis indicated that cow feces had the highest diversities in the bacterial and Bacteroidales group followed by the pig sample, with ... | 2011 | 21887641 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |