| tritium concentration in japanese rice. | polished rice samples harvested in 1985 were collected from 25 prefectures throughout japan. concentrations of both tissue free water tritium (tfwt) and organically bound tritium (obt) were determined. nearly uniform distribution of the tfwt and obt concentrations was observed in japan, taking into account a relatively large counting error. the average values for all japanese polished rice were 2.69 +/- 0.74 bq/l for the tfwt concentration, 2.01 +/- 0.78 bq/l for the obt concentration and 0.83 + ... | 1990 | 2098551 |
| 241am and plutonium in japanese rice-field surface soils. | | 1983 | 6663540 |
| marchiafava-bignami disease, striatal degeneration, and other neurological complications of chronic alcoholism in a japanese. | a japanese man with a variety of neurological complications, had drunk japanese rice wine (sake) daily for about 25 years. there was a progressive development of parkinsonism, cerebellar ataxia, and mental deterioration by the time he was 32. he died of pneumonia at age 50 and the autopsy revealed marchiafava-bignami disease (mbd), striatal degeneration, pseudolaminar sclerosis of morel, atrophy of the corpus mamillare and pons, cortical cerebellar atrophy, pseudopellagra, and polyneuropathy. th ... | 1981 | 7211193 |
| insect resistant rice generated by introduction of a modified delta-endotoxin gene of bacillus thuringiensis. | as a first step towards development of insect resistant rice we have introduced a truncated delta-endotoxin gene, cryia(b) of bacillus thuringiensis (b.t.) which has specific biological activity against lepidopteran insects into a japonica rice. to highly express the cryia(b) gene in rice the coding sequence was extensively modified based on the codon usage of rice genes. transgenic plants efficiently expressed the modified cryia(b) gene at both mrna and protein levels. bioassays using r2 genera ... | 1993 | 7764096 |
| efficient transformation of rice (oryza sativa l.) mediated by agrobacterium and sequence analysis of the boundaries of the t-dna. | a large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with agrobacterium tumefaciens. the efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium. stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the r0, r1 and r2 generations. sequence analysis revealed that the ... | 1994 | 7920717 |
| steroidal alkaloid toxicity to fish embryos. | embryos of two species of fish were evaluated for their suitability as model systems for steroidal alkaloid toxicity, the japanese rice fish, medaka (oryzius latipes) and the rainbow trout (oncorhynchus mykiss). additionally, the equine neurotoxic sesquiterpene lactone repin, was also tested. a probit program was used to evaluate the ec1, ec50 and ec99 as well as the associated confidence limits. the steroidal alkaloids tested were the solanum potato glycoalkaloids alpha-chaconine, alpha-solanin ... | 1993 | 8430437 |
| the japanese rice genome research program. | | 1996 | 8858341 |
| structure, chromosomal location and expression of a rice gene encoding the microsome omega-3 fatty acid desaturase. | the omega-3 fatty acid desaturases are membrane-bound enzymes catalyzing the conversion of linoleic acid to linolenic acid in lipids, and are located both in the microsome and plastid envelopes as two different isoforms. a cdna encoding the microsome omega-3 fatty acid desaturase (osfad3) and the corresponding genomic clone were isolated from rice (oryza sativa l.). the osfad3 gene was composed of 8 exons and 7 introns. a microsatellite was present in the second exon of the osfad3 gene, showing ... | 1997 | 9049269 |
| origin, dispersal, cultivation and variation of rice. | there are two cultivated and twenty-one wild species of genus oryza. o. sativa, the asian cultivated rice is grown all over the world. the african cultivated rice, o. glaberrima is grown on a small scale in west africa. the genus oryza probably originated about 130 million years ago in gondwanaland and different species got distributed into different continents with the breakup of gondwanaland. the cultivated species originated from a common ancestor with aa genome. perennial and annual ancestor ... | 1997 | 9291957 |
| rice genetic resources: history, conservation, investigative characterization and use in japan. | rice has been grown in japan for about 3000 years. although both japonica and indica varieties have been grown in japan, now japonica rices are grown. japanese rice breeding has used an ecological breeding approach. while emphasis in rice breeding in the 1940's and 1950's focussed on yield in recent decades quality has been of major importance. consumer preference and name recognition of high quality varieties, such as koshihikari, has resulted in slow acceptance of new varieties. rice germplasm ... | 1997 | 9291961 |
| agrobacterium-mediated transformation of élite indica and japonica rice cultivars. | a rapid, efficient, routine system has been established for agrobacterium tumefaciens-mediated production of hundreds of fertile transgenic plants from commercially important rice cultivars, including an indica cultivar, pusa basmati 1. calli induced from embryos of mature rice seeds were cocultivated with a. tumefaciens strain lba4404 carrying the plasmid ptok233, then exposed to hygromycin selection followed by an efficient regeneration system. based on the total number of calli co-cultivated, ... | 1997 | 9438257 |
| molecular characterization of two endogenous double-stranded rnas in rice and their inheritance by interspecific hybrids. | we completely sequenced 13,936 nucleotides (nt) of a double-stranded rna (dsrna) of wild rice (w-dsrna). a single long open reading frame (13,719 nt) containing the conserved motifs of rna-dependent rna polymerase and rna helicase was located in the coding strand. the identity between entire nucleotide sequence of w-dsrna and that of the dsrna of temperate japonica rice (j-dsrna, 13,952 nt) was 75.5%. a site-specific discontinuity (nick) was identified at nt 1,197 from the 5' end of the coding s ... | 1999 | 10066741 |
| purification, characterization, and application of an acid urease from arthrobacter mobilis. | it has been shown that urea in fermented beverages and foods can serve as a precursor of ethylcarbamate, a potential carcinogen, and acid urease is an effective agent for removing urea in such products. we describe herein the purification and characterization of a novel acid urease from arthrobacter mobilis sam 0752 and show its unique application for the removal of urea from fermented beverages using the japanese rice wine, sake, as an example. the purified acid urease showed an optimum ph for ... | 1999 | 10194859 |
| purification, characterization, and cdna structure of isoamylase from developing endosperm of rice. | isoamylase (ec 3.2.1.68) in rice (oryza sativa l.) was efficiently purified within a day to homogeneity, as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), from developing endosperm by sequential use of q sepharose hp anion-exchange chromatography, ammonium sulfate fractionation, and tskgel g4000swxl and g3000swxl gel filtration chromatography. although the protein exhibited a molecular size of ca. 83 kda on sds-page, the apparent size of the native enzyme was ... | 1999 | 10333591 |
| unusual inheritance of evolutionarily-related double-stranded rnas in interspecific hybrid between rice plants oryza sativa and oryza rufipogon. | endogenous, 14 kb double-stranded rnas (dsrnas) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, oryza sativa l.) and in wild rice (o. rufipogon, an ancestor of o. sativa). a comparison of the nucleotide and deduced amino acid sequences of the core regions of the rna-dependent rna polymerase domains found in these three dsrnas suggested that these dsrnas probably evolved independently within each host plant from a common ancestor. these ds ... | 1999 | 10380800 |
| [biological actions of acetaldehyde]. | acetaldehyde (ach), the first metabolite of ethanol (etoh), is a chemically reactive and pharmacologically active compound. the author has been engaged in the study of ach in cooperation with many researchers for three decades. we have found many biological actions of ach which cause cardiovascular symptoms after drinking and also inhibited etoh absorption via the canine and rat intestinal tract. this report covers the following five points. 1. the subjects were classified into a non-flushing gr ... | 1999 | 10723960 |
| identification of alpha-d-glucosylglycerol in sake. | alpha-d-glucosylglycerol (gg) was found for the first time in sake (japanese rice wine) in an amount of about 0.5%. gg was also found in miso and mirin which had been brewed by using koji. gg was hydrolyzed into glucose and glycerol in an equimolar ratio with maltase (ec 3.2.1.20, alpha-glucosidase from yeast), but not with emulsin (ec 3.2.1.21, beta-glucosidase from almond). the retention times and mass spectra of trimethylsilyl derivatives by a gc-ms analysis of gg in sake were comparable to t ... | 2000 | 10737196 |
| identification of a signal peptide for oryzacystatin-i. | a previously unidentified extension of an open reading frame from the genomic dna of japonica rice (oryza sativa l.) encoding oryzacystatin-i (oc-i; access. m29259, protein id aaa33912.1) has been identified as a 5' gene segment coding for the oc-i signal peptide. the signal peptide appears to direct a pre-protein (spoc-i; accession no. af164378) to the endoplasmic reticulum, where it is processed into the mature form of oc-i. the start codon of spoc-i begins 114 bp upstream from that previously ... | 2000 | 10805458 |
| ribozyme-mediated resistance to rice dwarf virus and the transgene silencing in the progeny of transgenic rice plants. | a hammerhead ribozyme (rz) with long hybridizing arms targeting the mrna of rice dwarf virus (rdv) segment 5 and a mutated nonfunctional ribozyme (mrz) were constructed. as predicted, rz transcribed in vitro cleaved the target mrna of rdv segment 5 into two fragments of 138 and 238 nucleotides in length. the rz and mrz genes were each placed under the control of the camv 35s promoter and used to transform japonica rice variety 'tongling no. 1' via agrobacterium tumefaciens. a total of 32 indepen ... | 2000 | 11032368 |
| characterization of acetic acid bacteria in traditional acetic acid fermentation of rice vinegar (komesu) and unpolished rice vinegar (kurosu) produced in japan. | bacterial strains were isolated from samples of japanese rice vinegar (komesu) and unpolished rice vinegar (kurosu) fermented by the traditional static method. fermentations have never been inoculated with a pure culture since they were started in 1907. a total of 178 isolates were divided into groups a and b on the basis of enterobacterial repetitive intergenic consensus-pcr and random amplified polymorphic dna fingerprinting analyses. the 16s ribosomal dna sequences of strains belonging to eac ... | 2001 | 11157275 |
| identification of yac clones containing the mutable slender glume locus slg in rice (oryza sativa l.). | a mutable slender glume gene slg, which often reverts to the wild-type state, was induced by gamma-ray irradiation of seeds of the japonica rice cultivar 'gimbozu'. the final goal was to understand whether the slender glume mutation was associated with the insertion of a transposable element, utilizing map-based cloning techniques. the rflp (restriction fragment length polymorphism) analysis revealed that the slg locus was located between two rflp loci, xnpb33 and r1440, on chromosome 7 with rec ... | 2001 | 11269342 |
| [improvement of transformation frequency of rice mediated by agrobacterium]. | the factors influencing the frequency of rice transformation mediated by agrobacterium have been investigated by using 16 commercially important indica and japonica rice cultivars or lines. the main results were as following: for most rice cc medium was the best for both callus initiation and subculture. with supplement of 2.5-5 mg/l aba the quality of calli can be improved. the concentration of selective agent for indica rice callus was lower than that for japonica rice callus. agrobacterium tu ... | 2001 | 11329877 |
| identification of defense-related rice genes by suppression subtractive hybridization and differential screening. | identification of host genes involved in defense responses is one of most critical steps leading to the elucidation of disease resistance mechanisms in plants. in this study, two different cloning strategies were employed to identify defense-related genes from a tropical japonica rice cultivar (oryza sativa cv. drew). with the use of bacterial colony arrays, differential screening of a blast fungus (pyricularia grisea)-induced rice cdna library led to the isolation of 22 distinct rice genes that ... | 2001 | 11332734 |
| two types of hkt transporters with different properties of na+ and k+ transport in oryza sativa. | it is thought that na+ and k+ homeostasis is crucial for salt-tolerance in plants. to better understand the na+ and k+ homeostasis in important crop rice (oryza sativa l.), a cdna homologous to the wheat hkt1 encoding k+-na+ symporter was isolated from japonica rice, cv nipponbare (ni-oshkt1). we also isolated two cdnas homologous to ni-oshkt1 from salt-tolerant indica rice, cv pokkali (po-oshkt1, po-oshkt2). the predicted amino acid sequence of ni-oshkt1 shares 100% identity with po-oshkt1 and ... | 2001 | 11489190 |
| establishment of a new cross of the rice blast fungus derived from japanese differential strain ina168 and hermaphroditic rice pathogen guy11. | mating experiments between magnaporthe grisea japanese rice pathogens and guy11, a hermaphroditic fertile rice pathogen, were done aimed at identification of avirulence genes. a cross named cross 2107 with thirty-six random progenies was obtained. segregation analyses of genetic markers found that the cross was less suitable for genetic analysis. backcrosses with cross 2107 progenies and guy11 were done and another cross named cross 5307 with sixty-five progenies was obtained. a locus controllin ... | 2001 | 11515527 |
| behaviors of d- and l-lactic acids during the brewing process of sake (japanese rice wine). | the amounts of d- and l-lactic acids during the brewing process of sake were determined by capillary electrophoresis using 2-hydroxypropyl-beta-cyclodextrin as a chiral selector. because l-lactic acid, which prevents the growth of nonuseful microorganisms, is a raw material of sake, the ratio of l-lactic acid to total lactic acid is almost 1.0 at the initial stage of sake brewing. during brewing, the ratio decreased gradually and finally reached 0.39. yeast (saccharomyces cerevisiae) for sake br ... | 2002 | 11829643 |
| identification of rice black streaked dwarf virus in different cereal crops with dwarfing symptoms in china. | this report describes isolation of virus particles from plants of rice, maize, wheat and sorghum with symptoms of dwarfing collected from two provinces of china, purification of double-stranded rna (dsrna) from the virus particles, and synthesis of full-length cdnas of genome segments 9 (s9) and 10 (s10) by reverse transcription-polymerase chain reaction (rt-pcr). sequence analysis showed that the s9 sequences of the chinese isolates and a japanese rice black-streaked disease virus (rbsdv) isola ... | 2001 | 12083334 |
| search for and analysis of single nucleotide polymorphisms (snps) in rice (oryza sativa, oryza rufipogon) and establishment of snp markers. | we searched for snps in 417 regions distributed throughout the genome of three oryza sativa ssp. japonica cultivars, two indica cultivars, and a wild rice (o. rufipogon). we found 2800 snps in approximately 250,000 aligned bases for an average of one snp every 89 bp, or one snp every 232 bp between two randomly selected strains. graphic representation of the frequency of snps along each chromosome showed uneven distribution of polymorphism-rich and -poor regions, but little obvious association w ... | 2002 | 12465716 |
| isolation, characterization and expression of the human factor in the germline alpha (figla) gene in ovarian follicles and oocytes. | the factor in the germline alpha (figalpha) transcription factor regulates expression of the zona pellucida proteins zp1, zp2 and zp3 and is essential for folliculogenesis in the mouse. using the published mouse figla sequence, blast searches identified a human chromosome 2 bac clone with high sequence identity. using pcr primers derived from this clone, amplicons derived from ovarian follicles and mature oocytes revealed 100% identity with the appropriate human bac clone, the expected homology ... | 2002 | 12468641 |
| [genetic analysis of blast resistance in japonica rice landrace heikezijing from taihu region]. | japonica rice landrace heikezijing (hkzj) from taihu region is highly resistant to several chinese and japanese differential strains of magnaporthe grisea. the f1, f2 and ril populations from the cross between the resistant variety heikezijing and the susceptible variety lijiangxintuanheigu (ljxthg) were inoculated by spray with two strains of ken 54-04 and hoku 1 in seedling stages. based on the r:s ratios of segregation in f1, f2 and ril populations it was showed that there were two independen ... | 2002 | 12561228 |
| inheritance and expression of the cry1ab gene in bt ( bacillus thuringiensis) transgenic rice. | the inheritance and expression patterns of the cry1ab gene were studied in the progenies derived from different bt ( bacillus thuringiensis) transgenic japonica rice lines under field conditions. both mendelian and distorted segregation ratios were observed in some selfed and crossed f(2) populations. crosses between japonica intra-subspecies had no significant effect on the segregation ratios of the cry1ab gene, but crossing between japonica and indicainter-subspecies led to distorted segregati ... | 2002 | 12582680 |
| genetic differentiation of wild relatives of rice as assessed by rflp analysis. | to study genetic diversity and relationships of wild relatives of rice, 58 accessions of oryza rufipogon, oryza nivara, oryza sativa f. spontanea and the cultivated oryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (rflp) technique. all 30-used rflp probes detected polymorphisms among the oryza accessions, with an average of 3.8 polymorphic fragments per probe. considerable genetic diversity was scored among the oryza ... | 2002 | 12582876 |
| transgenic expression of trypsin inhibitor cme from barley in indica and japonica rice, confers resistance to the rice weevil sitophilus oryzae. | indica and japonica rice (oryza sativa l.) plants were transformed by particle bombardment with the itr1 gene encoding the barley trypsin inhibitor bti-cme, under the control of its own promoter that confers endosperm specificity, and the maize ubiquitin promoter. from 38 independent transgenic lines of indica (breeding line ir58) and 15 of the japonica (cv senia) selected, 22 and 11, respectively, expressed the barley inhibitor at detectable levels. the transgene was correctly translated as ind ... | 2003 | 12650522 |
| stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. | amylose content in rice endosperm is a key determinant of eating and cooking quality. in the present study, a chimeric antisense construct, which contained a 756-bp antisense waxy (wx) gene dna fragment from rice and the gusa coding sequence, both fused to the 3.1-kb rice wx promoter, was efficiently introduced into several elite rice cultivars, both of japonica and indica type, via agrobacterium. more than 200 independent transgenic lines were produced and integration transgene was confirmed by ... | 2003 | 12650526 |
| molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. | hsp100 protein is an important component of the heat-shock response in diverse organisms. using specific primers based on cdna sequence, rice hsp101 gene was pcr-amplified and sequenced. southern analysis revealed that there appears to be a single gene per haploid genome coding for hsp101 protein in rice. northern analysis showed that expression of hsp101 transcript is strictly heat-inducible and induction is transient in nature. in the temperature regime tested, 45 degrees c treatment to intact ... | 2003 | 12650620 |
| methane emission from fields with differences in nitrogen fertilizers and rice varieties in taiwan paddy soils. | flooded rice fields are one of the major biogenic methane sources. in this study, methane emission rates were measured after transplanting in paddy fields with application of two kinds of nitrogen fertilizers (ammonium sulfate, nh4+-n and potassium nitrate, no3(-)-n) and with two kinds of rice varieties (japonica and indica). the experiment was conducted in fields located at tainan district agricultural improvement station in chia-yi county (23 degrees 25'08"n, 120 degrees 16'26"e) of southern t ... | 2003 | 12653295 |
| highly efficient production and characterization of t-dna plants for rice ( oryza sativa l.) functional genomics. | we investigated the potential of an improved agrobacterium tumefaciens-mediated transformation procedure of japonica rice ( oryza sativa l.) for generating large numbers of t-dna plants that are required for functional analysis of this model genome. using a t-dna construct bearing the hygromycin resistance ( hpt), green fluorescent protein ( gfp) and beta-glucuronidase ( gusa) genes, each individually driven by a camv 35s promoter, we established a highly efficient seed-embryo callus transformat ... | 2003 | 12677401 |
| inheritance of oryza sativa endornavirus in f1 and f2 hybrids between japonica and indica rice. | we have found a 14 kbp double-stranded rna (dsrna) in many cultivars of japonica rice (oryza sativa l.) but not in any cultivars of indica rice. this dsrna is an rna replicon with plasmid-like properties and is proposed to be a novel dsrna virus, oryza sativa endornavirus (osv). reciprocal crosses between the osv-carrier japonica variety (nipponbare) and the osv-free indica variety (ir 26 or kasalath) were performed to investigate whether osv can be transmitted to f1 hybrids. when ir 26 and nipp ... | 2003 | 12893964 |
| golden indica and japonica rice lines amenable to deregulation. | as an important step toward free access and, thus, impact of goldenrice, a freedom-to-operate situation has been achieved for developing countries for the technology involved. specifically, to carry the invention beyond its initial "proof-of-concept" status in a japonica rice (oryza sativa) cultivar, we report here on two transformed elite indica varieties (ir64 and mtl250) plus one japonica variety taipei 309. indica varieties are predominantly consumed in the areas with vitamin a deficiency. t ... | 2003 | 12970483 |
| [japanese rice in yeast diagnosis]. | | 1958 | 13501705 |
| use of asymmetric somatic hybridization for transfer of the bacterial blight resistance trait from oryza meyeriana l. to o. sativa l. ssp. japonica. | bacterial blight is one of the major diseases affecting rice productivity. to improve the resistance of cultivated rice to bacterial blight, we introduced a bacterial blight resistance trait from oryza meyeriana, a wild rice species, into an elite japonica rice cultivar (dalixiang) using asymmetric somatic hybridization. one hundred and thirty-two independent lines were regenerated. the hybrid plants possessed several morphological features of the donor species, o. meyeriana. random amplified po ... | 2004 | 14595515 |
| establishing an efficient ac/ds tagging system in rice: large-scale analysis of ds flanking sequences. | a two-element activator/dissociation (ac/ds) gene trap system was successfully established in rice (oryza sativa ssp. japonica cv. nipponbare) to generate a collection of stable, unlinked and single-copy ds transposants. the germinal transposition frequency of ds was estimated as an average of 51% by analyzing 4413 families. study of ds transposition pattern in siblings revealed that 79% had at least two different insertions, suggesting late transposition during rice development. analysis of 205 ... | 2004 | 14690513 |
| multiple tpr motifs characterize the fanconi anemia fancg protein. | the genome protection pathway that is defective in patients with fanconi anemia (fa) is controlled by at least eight genes, including brca2. a key step in the pathway involves the monoubiquitylation of fancd2, which critically depends on a multi-subunit nuclear 'core complex' of at least six fanc proteins (fanca, -c, -e, -f, -g, and -l). except for fancl, which has wd40 repeats and a ring finger domain, no significant domain structure has so far been recognized in any of the core complex protein ... | 2004 | 14697762 |
| [genetic analysis of a rolled-leaf mutant in rice population of t-dna insertion]. | a rolled-leaf mutant was obtained in a t-dna(containing bar gene and ds element) insertion population, which consist of transgenic japonica rice zhonghua 11 mediated by agrobacterium tumefaciens. through self-hybridization of three generations, one of trait-purified mutants (r1-a2) was obtained and used as parent to cross with variety zhonghua 11. the leaves of 36 f1 plants investigated were rolled and resistant to herbicide basta. among 852 f2 plants, the segregation ratio of rolled leaves to n ... | 2003 | 14724937 |
| relationship between the preference for sake (japanese rice wine) and the movements of metabolic parameters coinciding with sake intake. | selection of foods by animals largely depends on their physiological condition. in this paper, we reported how physiological changes in rats after ingestion of sake (japanese rice wine) affected their preference for different kinds of sake. rats could discriminate among various kinds of sakes in a two-bottle choice test, even after adjustment of the glucose concentration or alcohol concentration of the sake, suggesting that the choice of a sake by animals was based on more than one ingredient. t ... | 2004 | 15118305 |
| agrobacterium-mediated transformation of javanica rice cv. rojolele. | the agrobacterium-mediated transformation system was extended to a famous javanica rice variety, rojolele, that is cultivated in indonesia now. efficient callus induction from immature and mature seeds of rojolele did not succeed by any previous method for any rice cultivar. in this study, the callus from mature seeds of rojolele exhibited a compact and nodular appearance on c medium after the carbon source and medium ph was modified. scutellum-derived calli from mature seeds were co-cultivated ... | 2004 | 15215580 |
| optimising the tissue culture conditions for high efficiency transformation of indica rice. | establishment of high efficiency agrobacterium-mediated transformation techniques has greatly accelerated the widespread application of transformation in japonica rice. however, transformation in indica rice remains difficult. in this study, we identify two new media for subculture and differentiation, the two major steps in the tissue culture process for transformation. these media were tested using four cultivars representing very different germplasms of indica rice. the results show that the ... | 2005 | 15309499 |
| expression patterns of a novel atchx gene family highlight potential roles in osmotic adjustment and k+ homeostasis in pollen development. | a combined bioinformatic and experimental approach is being used to uncover the functions of a novel family of cation/h(+) exchanger (chx) genes in plants using arabidopsis as a model. the predicted protein (85-95 kd) of 28 atchx genes after revision consists of an amino-terminal domain with 10 to 12 transmembrane spans (approximately 440 residues) and a hydrophilic domain of approximately 360 residues at the carboxyl end, which is proposed to have regulatory roles. the hydrophobic, but not the ... | 2004 | 15347787 |
| production of bacterial blight resistant lines from somatic hybridization between oryza sativa l. and oryza meyeriana l. | novel bacterial blight (bb) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety oryza sativa (cv. 8411), via somatic hybridization using the wild rice oryza meyeriana as the donor of the resistance gene(s). twenty-nine progenies of somatically hybridized plants were obtained. seven somatically hybridized plants and their parents were used for aflp (amplified fragment length polymorphism) analysis using 8 primer pairs. results confirmed that these plants were ... | 2004 | 15362190 |
| genetic diversity and phylogeny of japanese sake-brewing rice as revealed by aflp and nuclear and chloroplast ssr markers. | japanese rice ( oryza sativa l.) cultivars that are strictly used for the brewing of sake (japanese rice wine) represent a unique and traditional group. these cultivars are characterized by common traits such as large grain size with low protein content and a large, central white-core structure. to understand the genetic diversity and phylogenetic characteristics of sake-brewing rice, we performed amplified fragment length polymorphism and simple sequence repeat analyses, using 95 cultivars of l ... | 2004 | 15375619 |
| potent antioxidative and antigenotoxic activity in aqueous extract of japanese rice bran--association with peroxidase activity. | to estimate the preventive potential of japanese rice bran (oryza sativa japonica) against the oxygen radical-related chronic diseases such as cardio-vascular diseases and cancer, antioxidative and antigenotoxic activities of the rice bran extracts were analyzed by using assay systems for lipid peroxidation and genotoxin-induced umu gene expression. when effects of the rice bran extracts under different extraction conditions on hydroperoxide generation from auto-oxidized linoleic acid were exami ... | 2004 | 15476307 |
| [physiological and genetic analysis of lesion resembling disease mutants (lrd) of oryza sativa l]. | ten indica rice and eight japonica rice mutants with lesion resembling disease (lrd27-44) were obtained by gamma-ray mutagenesis of the whole genomes. these mutants exhibited diverse lesion mimic phenotypes under different growth environments, could be accordingly classified two types, sensitive and insensitive to environments. basing on difference in development of lesion mimics, they can be divided into three categories: whole life lesion mimics (wllm), vegetative initiation lesion mimics (vil ... | 2004 | 15599031 |
| [analysis of the transgenic rice plants derived from transformed anther calli]. | rice calli derived from anther culture were used as recipient to transfer a rice blight resistance gene, xa21, into a japonica rice variety, taipei 309, via agrobacterium-mediated transformation. seven green transgenic plants, including one mixoploid, two haploid, and four diploid plants, were regenerated. pcr, southern blot, fish and blight resistance analysis all indicated that xa21 gene has been integrated into the t0 plant genomes. t1 generations of the four diploid t0 plants were further in ... | 2004 | 15633644 |
| [generation and identification of rice t-dna insertional mutant lines]. | by using the matured embryos of japonica rice variety zhonghua no. 11 as explants, rice transformation was performed by agrobacterium-mediated co-cultivation method, resulting in 1489 independent transgenic rice plants that carry a t-dna insertion. genomic dna gel-blot and pcr analyses showed that 69.8% of the total lines contain the inserted t-dna. the flanking sequence of t-dna in transgenic rice plants was analyzed using tail-pcr. in addition, we have evaluated 1066 t1 transgenic lines on hea ... | 2004 | 15633645 |
| ultraviolet-b sensitivities in japanese lowland rice cultivars: cyclobutane pyrimidine dimer photolyase activity and gene mutation. | there is a cultivar difference in the response to ultraviolet-b (uvb: 280-320 nm) in rice (oryza sativa l.). among japanese lowland rice cultivars, sasanishiki, a leading japanese rice cultivar, is resistant to the damaging effects of uvb while norin 1, a close relative, is less resistant. we found previously that norin 1 was deficient in cyclobutane pyrimidine dimer (cpd) photorepair ability and suggested that the uvb sensitivity in rice depends largely on cpd photorepair ability. in order to v ... | 2004 | 15653803 |
| the oslti6 genes encoding low-molecular-weight membrane proteins are differentially expressed in rice cultivars with contrasting sensitivity to low temperature. | rice (oryza sativa l.) is sensitive to chilling particularly at early stages of seedling establishment. two closely related genes (oslti6a, oslti6b), which are induced by low temperature during seedling emergence were isolated from a cold tolerant temperate japonica rice cultivar. these genes are closely related to the arabidopsis rare cold-inducible (rci2) and barley low-temperature-inducible (blt101) genes. based on direct biochemical and indirect physiological evidence and similarity with a c ... | 2005 | 15656983 |
| the genomes of oryza sativa: a history of duplications. | we report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. tested against a nonredundant collection of 19,079 full-length cdnas, 97.7% of the genes are aligned, without fragmentation, to the mapped super-scaffolds of one or the other genome. we introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous pred ... | 2005 | 15685292 |
| characterization of rat8 localization and mrna export in saccharomyces cerevisiae during the brewing of japanese sake. | ethanol affects the nuclear export of mrna in a similar way to heat shock in saccharomyces cerevisiae. we recently reported that the nuclear accumulation of rat8 caused by ethanol stress correlates well with blocking of the export of bulk poly(a)(+) mrna. here, we characterize the localization of rat8 and bulk poly(a)(+) mrna in sake (japanese rice wine) yeast during the brewing of sake. in wine must and synthetic dextrose medium, sake yeast showed the same responses to ethanol regarding changes ... | 2005 | 15803312 |
| essential amino acids of starch synthase iia differentiate amylopectin structure and starch quality between japonica and indica rice varieties. | four amino acids were variable between the 'active' indica-type and 'inactive' japonica-type soluble starch synthase iia (ssiia) of rice plants; glu-88 and gly-604 in ssiia of indica-cultivars ir36 and kasalath were replaced by asp-88 and ser-604, respectively, in both japonica cultivars nipponbare and kinmaze ssiia, whereas val-737 and leu-781 in indica ssiia were replaced by met-737 in cv. nipponbare and phe-781 in cv. kinmaze ssiia, respectively. the ssiia gene fragments shuffling experiments ... | 2005 | 16027975 |
| effect of gene disruptions of the tca cycle on production of succinic acid in saccharomyces cerevisiae. | succinate is the main taste component produced by yeasts during sake (japanese rice wine) fermentation. the pathway leading to accumulation of succinate was examined in liquid culture in the presence of a high concentration (15%) of glucose under aerobic and anaerobic conditions using a series of saccharomyces cerevisiae strains in which various genes that encode the expression of enzymes required in tca cycle were disrupted. when cultured in ypd medium containing 15% glucose under aerobic condi ... | 1999 | 16232421 |
| characterization of bacteriocin n15 produced by enterococcus faecium n15 and cloning of the related genes. | enterococcus faecium n15 was isolated from nuka (japanese rice-bran paste), which is utilized as starter in the fermenting of vegetables, and was found to produce a bacteriocin that exhibited a broad spectrum of activity, including activity against listeria monocytogenes and bacillus circulans jcm2504. the bacteriocin was sensitive to proteases (alpha-chymotrypsin, proteinase k, trypsin, and pepsin) and alpha-amylase, but it was resistant to lipase. the bacteriocin was resistant to heat treatmen ... | 2001 | 16233010 |
| [agrobacterium-mediated transformation of rice mature embryos and regeneration of transgenic plants with metr gene]. | the mature embryos of a japonica rice, guanglingxiangnuo, were used for the study on optimization of agrobacterium-mediated transformation. several factors affecting the transformation were investigated and a suitable transformation system was set up. for this transformation system, the hrm medium, based on the ms medium, was suitable for inducing callus from rice mature embryos. the suitable time span of initial culture in this medium was 7-8 days before co-culturing with agrobacterium and suit ... | 2005 | 16257907 |
| [characteristics of yunnan province rice landrace core collections under phosphorus deficiency]. | soil phosphorus (p) deficiency is a major yield-limiting factor in rice production. employing 526 rice landrace accessions from 5 rice-planting regions and 16 prefectures in yunnan province, this paper studied their p-deficiency tolerance characteristics by comparing the relative plant dry weight under acid red soil condition. the results showed that northwest yunnan cold highland japonica rice-planting region was most abundant in p-deficiency tolerance core collection of indica, and there was n ... | 2005 | 16262081 |
| cadmium and lead contamination in japonica rice grains and its variation among the different locations in southeast china. | on the basis of a large-scale survey with 269 sampling sites and a field experiment with 12 cultivars grown at 3 ecologically different locations, cadmium (cd) and lead (pb) contamination in milled rice grains and its variation among different locations were investigated in the area of southeast china. the objective of this study was mainly to assess the present situation of cd and pb contamination in rice grains harvested locally and find out the variation of cd and pb background levels in rice ... | 2006 | 16266740 |
| large-scale systematic study on stability of the ds element and timing of transposition in rice. | activator/dissociation (ac/ds) transposon mutagenesis is a widely used tool for gene identification; however, several reports on silencing of the ac/ds element in starter lines and in stable transposants question the applicability of such an approach in later generations. we have performed a systematic analysis on various aspects of the silencing phenomenon in rice (oryza sativa ssp. japonica cv. nipponbare). high somatic and germinal transposition frequencies observed in earlier generations wer ... | 2006 | 16275658 |
| [establishment of promoter trapping system mediated by activator/dissociation (beta-glucuronidase) construction in rice]. | the coding region of bar gene, the left border of ds element, the coding region of gus gene, the transposase of ac element, the right border of ds element and the promoter of ubi gene were inserted into the t-dna region of vector pcambia1300 in turn to construct plasmid p13b. the orientations of the ubiquitons' promoter, ac transposase and bar are identical but opposite to that of the gus gene (fig.1). the plasmid p13b was then introduced into the calli of oryza sativa subsp. japonica cv. zhongh ... | 2005 | 16361783 |
| genealogy of the "green revolution" gene in rice. | during the "green revolution" of rice, high-yielding varieties (hyvs) were developed using a semi-dwarf gene (sd1 or osga20ox2). the presence or absence of the two mutant alleles (dgwg type in dee-geo-woo-gen and jkk type in jikkoku) were surveyed by pcr using 256 accessions of eight wild and two cultivate rice species. the dgwg allele was detected in a landrace (oryza sativa) and two accessions of wild rice (o. rufipogon), all of which are from china, showing their limited distribution. genealo ... | 2005 | 16394586 |
| [expression and immunization testing of fusion protein of newcastle disease virus in leaf tissue of transgenic rice]. | transgenic plant is an attractive bioreactor to produce recombinant protein, such as safe and economic vaccine. in present study, a plant expression binary vector pundv, containing the 1.7 kb fusion protein gene of newcastle disease virus (ndv f) under the control of maize ubiquitin (ubi) promoter and nos terminator was constructed, in which, the hygromycin phosphotransferase (hpt) gene was used as the selectable marker. the expression cassette was introduced into a japonica rice variety by agro ... | 2005 | 16459660 |
| development of transformation system of rice based on binary bacterial artificial chromosome (bibac) vector. | an agrobacterium-mediated transformation protocol using binary bacterial artificial chromosome (bibac) vector system in rice (oryza sativa l.) was developed. calli derived from mature embryos of japonica rice cv. h1493 were used as target tissues. various aspects in transformation and regeneration processes including callus induction and culture, agrobacterium concentration and duration of co-cultivation, bacterial elimination and transformant selection were examined in order to improve the tran ... | 2006 | 16553216 |
| phylogeography of asian wild rice, oryza rufipogon, reveals multiple independent domestications of cultivated rice, oryza sativa. | cultivated rice, oryza sativa l., represents the world's most important staple food crop, feeding more than half of the human population. despite this essential role in world agriculture, the history of cultivated rice's domestication from its wild ancestor, oryza rufipogon, remains unclear. in this study, dna sequence variation in three gene regions is examined in a phylogeographic approach to investigate the domestication of cultivated rice. results indicate that india and indochina may repres ... | 2006 | 16766658 |
| expression of rice yellow mottle virus coat protein enhances virus infection in transgenic plants. | the disease caused by rice yellow mottle virus (rymv) is a major, economically important constraint to rice production in africa. rymv is mechanically transmitted by a variety of agents, including insect vectors. the production of resistant rice varieties would be an important advance in the control of the disease and increase rice production in africa. we produced transgenic plants of the oryza sativa japonica variety, tp309, to express a rymv coat protein gene (cp) and mutants of the cp under ... | 2006 | 16773235 |
| [raising fat content in transgenic rice by anti-pep gene transformation]. | gene for phosphoenolpyruvate carboxylase (pepcase) was incorporated in antisense orientation into genome of japonica rice variety xiushui 11 through agrobacterium tumefaciens-mediated transformation of mature seeds-derived embryogenic calli, and a total of 95 transgenic plants were regenerated, which were confirmed by histochemical detection of gus activity (fig.2), pcr assay (fig.3) and southern hybridization analysis (fig.4). the transgene was inherited by the t1 progeny and mendelian 3:1 segr ... | 2006 | 16775403 |
| environmental factors that affect the ability of amylose to contribute to retrogradation in gels made from rice flour. | retrogradation in rice is a trait that describes the hardening of cooked rice after storage or cooling, and it has significant implications for many consumers of rice, since many people cook rice in the morning and consume it several hours later or the next day. tools to select against retrogradation in breeding programs are yet to be described. here, we aim to determine the effect on retrogradation of storage time and temperature and the role of starch, protein, and lipids using gels made from ... | 2006 | 16819933 |
| rice (oryza sativa l.) inhibits growth and induces differentiation of human leukemic u937 cells through activation of peripheral blood mononuclear cells. | rice (oryza sativa l.) is a staple food worldwide for centuries. in this study, the growth-inhibiting and immunopotentiating effects of commonly used rice were examined. the growth of human leukemic u937 cells was significantly inhibited by the peripheral blood mononuclear cell-conditioned medium (mnc-cm) derived from water extracts of japonica rice milled taiwan 9 (mt9) and brown taiwan 9 (bt9). furthermore, these mnc-cms induced differentiation of u937 cells into mature monocytes/macrophages e ... | 2006 | 16842898 |
| in vitro bactericidal activities of japanese rice-fluid against helicobacter pylori strains. | helicobacter pylori has now been widely recognized as a causative agent of gastroduodenal diseases. the development of safer anti- h. pylori compounds is desirable due to the antibiotic-resistant strains emerged to date. | 2006 | 16906220 |
| japonica rice varieties (oryza sativa, nipponbare, and others). | agrobacterium-mediated transformation of rice is now used in many laboratories worldwide. several protocols have been developed and fine-tuned for particular genotypes, including commercial genotypes, making use of either mature seeds or immature embryos as target tissue for agrobacterium infection. in this chapter, we describe a rapid and user-friendly protocol based on mature seeds that can deliver transgenic rice plant-lets within 2 mo. the protocol described is based on the use of the nptii ... | 2006 | 16988346 |
| [a novel method of the genome-wide prediction for the target genes and its application]. | based on the protein databases of several model species, this study developed a new method of the genome-wide prediction for the target genes, using hidden markov model by perl programming. the advantages of this method are high throughput, high quality and easy prediction, especially in the case of multi-domains proteins families. by this method, we predicted the ppr and tpr proteins families in whole genome of several model species. there were 536 ppr proteins and 199 tpr proteins in oryza sat ... | 2006 | 17035191 |
| adapting rice anther culture to gene transformation and rna interference. | anther culture offers a rapid method of generating homozygous lines for breeding program and genetic analysis. to produce homozygous transgenic lines of rice (oryza sativa l.) in one step, we developed an efficient protocol of anther-callus-based transformation mediated by agrobacterium after optimizing several factors influencing efficient transformation, including callus induction and agrobacterium density for co-cultivation. using this protocol, we obtained 145 independent green transformants ... | 2006 | 17172048 |
| marker-free transgenic (mft) near-isogenic introgression lines (niils) of 'golden' indica rice (cv. ir64) with accumulation of provitamin a in the endosperm tissue. | we have developed near-isogenic introgression lines (niils) of an elite indica rice cultivar (ir64) with the genes for beta-carotene biosynthesis from dihaploid (dh) derivatives of golden japonica rice (cv. t309). a careful analysis of the dh lines indicated the integration of the genes of interest [phytoene synthase (psy) and phytoene desaturase (crti)] and the selectable marker gene (hygromycin phosphotransferase, hph) in two unlinked loci. during subsequent crossing, progenies could be obtain ... | 2006 | 17177811 |
| multilocus analysis of nucleotide variation of oryza sativa and its wild relatives: severe bottleneck during domestication of rice. | varying degrees of reduction of genetic diversity in crops relative to their wild progenitors occurred during the process of domestication. such information, however, has not been available for the asian cultivated rice (oryza sativa) despite its importance as a staple food and a model organism. to reveal levels and patterns of nucleotide diversity and to elucidate the genetic relationship and demographic history of o. sativa and its close relatives (oryza rufipogon and oryza nivara), we investi ... | 2007 | 17218640 |
| effects of rice proteins from two cultivars, koshihikari and shunyo, on cholesterol and triglyceride metabolism in growing and adult rats. | the effect and mechanism of two types of rice protein, one from regular japonica rice koshihikari and another from rice cultivar shunyo, with low glutelin and high prolamin content, on cholesterol and triglyceride metabolism were compared by feeding casein and soy protein to male wistar strain rats 7 and 20 weeks old ad libitum for 2 weeks. the results in adult rats clearly indicated that both rice proteins had cholesterol-lowering effects in the plasma and the liver, comparable to soy protein, ... | 2007 | 17341819 |
| molecular characterization of t4-type bacteriophages in a rice field. | bacteriophages, the viruses that infect bacteria, are the most abundant biological entities in the biosphere and play a key role in global biogeochemical cycling. all t4-type bacteriophage isolates tested so far have a conserved genetic module that encodes the virion components including gene 23 (g23), the major capsid protein. molecular analysis of the g23 sequence revealed a remarkable level of diversity of t4-type bacteriophages isolated from rice straw and surface soil in a japanese rice fie ... | 2007 | 17359280 |
| characterization of a lipoate-protein ligase a gene of rice (oryza sativa l.). | lipoic acid is an essential disulfide cofactor required for the lipoate-dependent enzymes including pyruvate dehydrogenase (pdh), alpha-ketoglutarate dehydrogenase (kgdh), and glycine cleavage enzymes that function in key metabolic pathways in most prokaryotes and eukaryotes. lipoic acid is covalently bound to lipoate-dependent enzymes by lipoate-protein ligase or lipoate transferase. here, we characterized a lipoyl-protein ligase a (oslpla) gene of rice. the oslpla gene, which encoded 270 amino ... | 2007 | 17376611 |
| a protocol for agrobacterium-mediated transformation in rice. | agrobacterium-mediated transformation of rice is an important method that has been widely adopted by many laboratories. however, because current approaches rely on culture systems, routine protocols have been established only in japonica rice, especially those varieties with higher regeneration potential. some very efficient methods have been developed for japonica varieties that enable high-throughput functional analysis in rice; however, many elite japonica, and most indica, varieties are diff ... | 2006 | 17406537 |
| direct electrical power generation from urine, wastes and biomass with simultaneous photodecomposition and cleaning. | electric power was for the first time generated directly from urine, wastes, and biomass with simultaneous photodecomposition and cleaning by using a biophotofuel cell (bpfc) composed of a nanoporous tio2 film semiconductor photoanode and an o2-reducing cathode. human urine exhibited a pfc characteristics with j(sc) 0.086 ma cm(-2), voc 0.56 v, and fill factor (ff) 0.50 under irradiation by a solar simulator with am 1.5 g and 100 mw cm(-2) incident light intensity. both the soluble and residual ... | 2007 | 17467260 |
| [quick testing-technology of transgenic rice with npt- screen marker gene]. | by using npt-gene as assistant selection-marker, treating japonica rice zhonghua 9 [zh9 (ck)] and transferred lysozyme gene rice (the donor rice is japonica rice zhonghua 9) [zh9(r)] with antibiotics, we built a system of quickly testing transgenic rice offspring. detached leaves of zh9(r) and zh9(ck) were treated by kanamycin (0, 400, 450, 500, 550, 600 and 700 mg/l) and g418 (0, 40, 60, 80, 100, 150 and 200 mg/l) with different concentrations. the result show effect of kanamycin is not evident ... | 2007 | 17548315 |
| epigenetic inheritance in rice plants. | epigenetics is defined as mechanisms that regulate gene expression without base sequence alteration. one molecular basis is considered to be dna cytosine methylation, which reversibly modifies dna or chromatin structures. although its correlation with epigenetic inheritance over generations has been circumstantially shown, evidence at the gene level has been limited. the present study aims to find genes whose methylation status directly correlates with inheritance of phenotypic changes. | 2007 | 17576658 |
| identification of target genes conferring ethanol stress tolerance to saccharomyces cerevisiae based on dna microarray data analysis. | during industrial production process using yeast, cells are exposed to the stress due to the accumulation of ethanol, which affects the cell growth activity and productivity of target products, thus, the ethanol stress-tolerant yeast strains are highly desired. to identify the target gene(s) for constructing ethanol stress tolerant yeast strains, we obtained the gene expression profiles of two strains of saccharomyces cerevisiae, namely, a laboratory strain and a strain used for brewing japanese ... | 2007 | 17604866 |
| in vivo bactericidal activities of japanese rice-fluid against h. pylori in a mongolian gerbil model. | the antibiotic effect of rice-fluid on helicobacter pylori infection was investigated using a mongolian gerbil model. | 2007 | 17717596 |
| the expression pattern of a rice disease resistance gene xa3/xa26 is differentially regulated by the genetic backgrounds and developmental stages that influence its function. | genetic background and developmental stage influence the function of some disease resistance (r) genes. the molecular mechanisms of these modifications remain elusive. our results show that the two factors are associated with the expression of the r gene in rice xa3 (also known as xa26)-mediated resistance to xanthomonas oryzae pv. oryzae (xoo), which in turn influences the expression of defense-responsive genes. the background of japonica rice, one of the two major subspecies of asian cultivate ... | 2007 | 17720929 |
| [genetic analysis of resistance to rice blast in four japonica landraces from taihu lake region]. | the crosses were made between four japonica rice (oryza sativa l.) landraces bodao, tieganqing, jiangnanwan and queernuo from taihu lake region, which were highly resistant to the blast (magnaporthe grisa), and a susceptible japonica variety suyunuo to produce f1 and f2 generations. the p1 , p2, f1 and f2 generation from various combinations were inoculated separately with japanese blast strain hoku 1 and chinese races ze3 and zg1 to study genetic patterns of resis-tance in the four landraces to ... | 2007 | 17905716 |
| tac1, a major quantitative trait locus controlling tiller angle in rice. | a critical step during rice (oryza sativa) cultivation is dense planting: a wider tiller angle will increase leaf shade and decrease photosynthesis efficiency, whereas a narrower tiller angle makes for more efficient plant architecture. the molecular basis of tiller angle remains unknown. this research demonstrates that tiller angle is controlled by a major quantitative trait locus, tac1 (tiller angle control 1). tac1 was mapped to a 35-kb region on chromosome 9 using a large f(2) population fro ... | 2007 | 17908158 |
| an empirical model of soil chemical properties that regulate methane production in japanese rice paddy soils. | to understand which soil chemical properties are the best predictors of ch4 production in rice paddy soils, a model was developed with empirical data from nine types of rice soils collected around japan and anaerobically incubated at 30 degrees c for 16 wk in laboratory conditions. after 1, 2, 4, 8, and 16 wk of incubation, co2, ch4, and fe(ii) were measured to understand soil organic matter decomposition and iron (fe) reduction. available n (n ava) was also measured at the end of incubation. th ... | 2007 | 17965395 |
| characterization of transcription factor gene snac2 conferring cold and salt tolerance in rice. | plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. nac (nam, ataf, and cuc) is a plant specific transcription factor family with diverse roles in development and stress regulation. in this report, a stress-responsive nac gene (snac2) isolated from upland rice ira109 (oryza sativa l. ssp japonica) was characterized for its role in stres ... | 2008 | 18273684 |
| gene structure dynamics and divergence of the polygalacturonase gene family of plants and fungus. | whole copies of the polygalacturonase (pg) genes from rice (oryza sativa subsp. japonica) and a filamentous fungus (aspergillus oryzae) were isolated. the orthologs of the rice pgs were also retrieved from other plant species. the 106 plant pgs analyzed were divided into 5 clades, a, b, c, d, and e. the fungus pgs were classified into 3 clades, of which one formed a loose cluster with clade e of the plant pgs. four domain motifs (i, ii, iii, iv) were identified in all pgs. motifs ii and iii were ... | 2008 | 18356937 |
| development of near-isogenic japonica rice lines with enhanced resistance to magnaporthe grisea. | thirteen near-isogenic lines (nils) of japonica rice were developed via a backcross method using the recurrent parent chucheong, which is of good eating quality but is susceptible to magnaporthe grisea, and three blast resistant japonica donors, seolak, daeseong and bongkwang. the agro-morphological traits of these nils, such as heading date, culm length, and panicle length, were similar to those of chucheong. in a genome-wide scan using 158 ssr markers, chromosome segments of chucheong were ide ... | 2008 | 18443412 |
| extraordinarily polymorphic ribosomal dna in wild and cultivated rice. | a collection of 481 rice accessions was surveyed for ribosomal dna (rdna) intergenic spacer length polymorphism to assess the extent of genetic diversity in chinese and asian rice germplasm. the materials included 83 accessions of common wild rice, oryza rufipogon, 75 of which were from china; 348 entries of cultivated rice (oryza sativa), representing almost all the rice growing areas in china; and 50 cultivars from south and east asia. a total of 42 spacer length variants (slvs) were detected. ... | 1996 | 18469959 |
| pathogenesis-related proteins in somatic hybrid rice induced by bacterial blight. | rice bacterial blight, caused by xanthomonasoryzae pv. oryzae (xoo), is one of the most serious rice diseases worldwide. the bacterial blight resistance trait from oryza meyeriana, a wild rice species, was introduced into an elite japonica rice cultivar using asymmetric somatic hybridization. this study was carried out with the intention of understanding the molecular mechanism of incompatible interaction between xoo and the stable somatic hybrids by using proteomic analyses. proteins were extra ... | 2008 | 18534637 |
| [construction and analysis of cdna library of yunnan yuanjiang o. rifupogon leaf]. | the cdna library of yuanjiang oryza rifupongon leaf was constructed by using smart technology. the titers of the non-amplified library and the amplified library were 1.1 x 106 pfu/ml and 3.98 x 107 pfu/ml, respectively. the recombination rate was more than 91%. the dna sequence length of the most cdnas in the library was between 500-2 000 bp. some cdnas chosen by random were sequenced. after blast analysis of some cdnas, their possible function were predicted. it is found that these cdnas show 9 ... | 2008 | 18550503 |
| expression analysis of rice a20/an1-type zinc finger genes and characterization of zfp177 that contributes to temperature stress tolerance. | the a20/an1-type zinc finger protein family is conserved in animals and plants. using human awp1 protein as a query, we identified twelve a20/an1-type zinc finger proteins in japonica rice. most of these genes were constitutively expressed in leaves, roots, culms and spikes. through microarray analysis, it was found that four genes (zfp177, zfp181, zfp176, zfp173), two genes (zfp181 and zfp176) and one gene (zfp157) were significantly induced by cold, drought and h(2)o(2) treatments, respectivel ... | 2008 | 18588956 |