| suitability of full fat and defatted rice brain obtained from indian rice for use in food products. | the effect of blending different types of rice bran in wheat flour on the rheological, pasting and baking properties is reported. dough development time and strength as measured through farinograph decreased with the blending of unstabilized full fat and defatted bran; whereas, blending of stabilized full fat and defatted bran improved the dough strength. pasting properties revealed an increase in gelatinization temperature and decrease in paste viscosities and set-back values with the blending ... | 1995 | 7659697 |
| transgenic indica rice breeding line ir58 expressing a synthetic cryia(b) gene from bacillus thuringiensis provides effective insect pest control. | the indica rice breeding line ir58 was transformed by particle bombardment with a truncated version of a synthetic cryia(b) gene from bacillus thuringiensis. this gene is expressed under control of the camv 35s promoter and allows efficient production of the lepidopteran specific delta-endotoxin. r0, r1 and r2 generation plants displayed a significant insecticidal effect on several lepidopterous insect pests. feeding studies showed mortality rates of up to 100% for two of the most destructive in ... | 1996 | 9636319 |
| [improvement of transformation frequency of rice mediated by agrobacterium]. | the factors influencing the frequency of rice transformation mediated by agrobacterium have been investigated by using 16 commercially important indica and japonica rice cultivars or lines. the main results were as following: for most rice cc medium was the best for both callus initiation and subculture. with supplement of 2.5-5 mg/l aba the quality of calli can be improved. the concentration of selective agent for indica rice callus was lower than that for japonica rice callus. agrobacterium tu ... | 2001 | 11329877 |
| development of new transformation-competent artificial chromosome vectors and rice genomic libraries for efficient gene cloning. | the transformation-competent artificial chromosome vector (tac) system has been shown to be very useful for efficient gene isolation in arabidopsis thaliana (proc. natl. acad. sci. usa 96 (1998) 6535). to adapt the vector system for gene isolation in crops, two new tac vectors and rice genomic libraries were developed. the new vectors pyltac17 and pyltac27 use the bar gene and hpt gene driven by the rice act1 promoter as the plant selectable markers, respectively, and are suitable for transforma ... | 2002 | 11814697 |
| [identification of a resistance gene to bacterial blight (xanthomonas oryzae pv. oryzae) in a somaclonal mutant hx-3 of indica rice]. | using the mature embryo of a susceptible rice variety minghui 63 as the explant, we have obtained a somaclonal mutant hx-3 through selection in vitro, which has showed resistance to bacterial blight. in 8 successive years, the resistance of r1 to r9 generations of hx-3 was identified by zj173, a typical bacterial blight strain in yangtsu river valley, and the results showed that the resistance of hx-3 was stable and heritable. genetic analysis also indicated that the resistance of hx-3 to bacter ... | 2002 | 11901997 |
| [callus induction and regeneration from mature seeds of indica rice minghui 63 and anti-fungal assay of transgenic rice plants]. | a large number of callus from mature seeds of indica rice minghui 63 were obtained through pre-induction on medium with 2 mg/l 2,4-d but without inorganic and organic components for 9 days. trichosanthin gene was transferred into indica rice minghui 63 by using agrobacterium with the help of bombardment and the transgenic plants were obtained by inducing regeneration. southern and western blot analysis showed that the trichosanthin gene had been transferred into genome of minghui 63 and expresse ... | 2002 | 12192866 |
| elite indica transgenic rice plants expressing modified cry1ac endotoxin of bacillus thuringiensis show enhanced resistance to yellow stem borer (scirpophaga incertulas). | bt-transgenics of elite indica rice breeding lines (ir-64, pusa basmati-1 and karnal local) were generated through biolistic or agrobacterium-mediated approaches. a synthetic cry1ac gene, codon optimised for rice and driven by the maize ubiquitin-1 promoter, was used. over 200 putative transformants of ir-64 and pusa basmati-1 and 26 of the karnal local were regenerated following use of the hpt (hygromycin phosphotransferase) selection system. initial transformation frequency was in the range of ... | 2002 | 12212843 |
| syngenta ready to drop plans for indian rice venture. | | 2002 | 12478254 |
| transgenics of an elite indica rice variety pusa basmati 1 harbouring the coda gene are highly tolerant to salt stress. | transgenic lines of indica rice were generated by agrobacterium-mediated transformation with the choline oxidase ( coda) gene from arthrobacter globiformis. choline oxidase catalyses conversion of choline to glycine betaine. glycine betaine is known to provide tolerance against a variety of stresses. molecular analyses of seven independent transgenic lines as performed by southern, northern and western hybridization revealed integration and expression of the transgene as well as inheritance in t ... | 2002 | 12582870 |
| stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. | amylose content in rice endosperm is a key determinant of eating and cooking quality. in the present study, a chimeric antisense construct, which contained a 756-bp antisense waxy (wx) gene dna fragment from rice and the gusa coding sequence, both fused to the 3.1-kb rice wx promoter, was efficiently introduced into several elite rice cultivars, both of japonica and indica type, via agrobacterium. more than 200 independent transgenic lines were produced and integration transgene was confirmed by ... | 2003 | 12650526 |
| molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. | hsp100 protein is an important component of the heat-shock response in diverse organisms. using specific primers based on cdna sequence, rice hsp101 gene was pcr-amplified and sequenced. southern analysis revealed that there appears to be a single gene per haploid genome coding for hsp101 protein in rice. northern analysis showed that expression of hsp101 transcript is strictly heat-inducible and induction is transient in nature. in the temperature regime tested, 45 degrees c treatment to intact ... | 2003 | 12650620 |
| methane emission from fields with differences in nitrogen fertilizers and rice varieties in taiwan paddy soils. | flooded rice fields are one of the major biogenic methane sources. in this study, methane emission rates were measured after transplanting in paddy fields with application of two kinds of nitrogen fertilizers (ammonium sulfate, nh4+-n and potassium nitrate, no3(-)-n) and with two kinds of rice varieties (japonica and indica). the experiment was conducted in fields located at tainan district agricultural improvement station in chia-yi county (23 degrees 25'08"n, 120 degrees 16'26"e) of southern t ... | 2003 | 12653295 |
| expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. | rice hoja blanca virus (rhbv) is a major virus disease of economic importance affecting rice in northern south america, central america and the caribbean. this is the first report of transgenic resistance to rhbv and the transformation of an indica rice variety from latin america. rice transformed with the rhbv nucleocapsid protein ( n) gene had a significant reduction in disease development. several reactions were observed that ranged from susceptible to completely resistant plants (immunity). ... | 2003 | 12671749 |
| investigating options for attenuating methane emission from indian rice fields. | the development of methods and strategies to reduce the emission of methane from paddy fields is a central component of ongoing efforts to protect the earth's atmosphere and to avert a possible climate change. it appears from this investigation that there can be more than one strategy to contain methane emission from paddy fields, which are thought to be a major source of methane emission in tropical asia. promising among the mitigating options may be water management, organic amendments, fertil ... | 2003 | 12742397 |
| analysis of n-acyl-l-homoserine lactones produced by burkholderia cepacia with partial filling micellar electrokinetic chromatography--electrospray ionization-ion trap mass spectrometry. | a method for the analysis of n-acyl-l-homoserine lactones (ahls) with micellar electrokinetic chromatography coupled to electrospray ionization-ion trap mass spectrometry, combining the flexibility of capillary electrophoresis with the unmatched structural information provided by mass spectrometry is presented. different surfactants were evaluated, with sodium dodecyl sulfate (sds) yielding the best results considering sensitivity and flexibility. we examined the interaction of ahls with the sds ... | 2003 | 12973811 |
| rice proteomics: current status and future perspectives. | rice, the first cereal crop genome to be decoded, has attracted the attention of researchers worldwide because of its immense socio-economic impact on human existence. with the availability of the draft genome sequence of two major types, japonica- and indica-rice, "rice proteomics" has entered into the era of functional genomics. although during the last decade an important but limited progress (mainly construction of protein datafiles) has been made in the field of rice proteomics, it is only ... | 2003 | 14595685 |
| [the starchy endosperm denucleation by a process of programmed cell death during rice grain development]. | the cellular ultrastructural morphology, grain filling ratio and activity of related enzymes during denucleation development stage in starchy endosperm cell of an indica rice variety zhongxian 8836 were observed and analyzed. the endosperm cellularization was completed at about the 3rd day after flowering (daf). at 5 daf a few endosperm cells initiated denucleation development. at 13 daf almost all the starchy endosperm cells completed their denucleation. the nuclear degradation is the first sta ... | 2004 | 15133896 |
| agrobacterium-mediated transformation of bangladeshi indica rices. | morphologically normal, fertile transgenic plants were obtained by co-culturing embryogenic calli of the bangladeshi indica rice cultivars br26 and binni with agrobacterium tumefaciens strain lba4404 carrying the super binary vector ptok233. acetosyringone (100 microm) in the medium during co-culture (25-28 degrees c) and selection on hygromycin b (50 mg l(-1)) were essential for efficient transformation. stable integration and expression of beta-glucuronidase, neomycin phosphotransferase and hy ... | 2004 | 15213809 |
| optimising the tissue culture conditions for high efficiency transformation of indica rice. | establishment of high efficiency agrobacterium-mediated transformation techniques has greatly accelerated the widespread application of transformation in japonica rice. however, transformation in indica rice remains difficult. in this study, we identify two new media for subculture and differentiation, the two major steps in the tissue culture process for transformation. these media were tested using four cultivars representing very different germplasms of indica rice. the results show that the ... | 2005 | 15309499 |
| [transferring of multiple insect-resisting genes into two-line genetic male sterile rice pei'ai 64s by particle bombardment]. | transformation of two-line genetic male sterile indica rice variety pei'ai 64s was conducted by particle bombardment. three insect-resisting genes ligased into plasmid vector pkc-3 was introduced into calli derived from mature embryo and a total of 33 transgenic plants had been obtained. the pcr and southern blot analysis of each different gene in r0 plants and also pcr analysis of each different gene in r1 plants showed the integration of three insect-resisting genes into the genome of transgen ... | 2002 | 15344316 |
| essential amino acids of starch synthase iia differentiate amylopectin structure and starch quality between japonica and indica rice varieties. | four amino acids were variable between the 'active' indica-type and 'inactive' japonica-type soluble starch synthase iia (ssiia) of rice plants; glu-88 and gly-604 in ssiia of indica-cultivars ir36 and kasalath were replaced by asp-88 and ser-604, respectively, in both japonica cultivars nipponbare and kinmaze ssiia, whereas val-737 and leu-781 in indica ssiia were replaced by met-737 in cv. nipponbare and phe-781 in cv. kinmaze ssiia, respectively. the ssiia gene fragments shuffling experiments ... | 2005 | 16027975 |
| transgenic indica rice plants harboring a synthetic cry2a* gene of bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests. | a novel synthetic cry2a* gene was introduced into the elite indica rice restorer line minghui 63 by agrobacterium-mediated transformation. a total of 102 independent transformants were obtained. among them, 71 transformants were positive cry2a* plants according to pcr analysis. four highly insect-resistant lines with single-copy insertion (designated as 2a-1, 2a-2, 2a-3, and 2a-4) were selected based on field assessment and southern blot analysis in the t(1) generation. all four transgenic lines ... | 2005 | 16187120 |
| transgenic rice expressing allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests. | mannose binding allium sativum leaf agglutinin (asal) has been shown to be antifeedant and insecticidal against sap-sucking insects. in the present investigation, asal coding sequence was expressed under the control of camv35s promoter in a chimeric gene cassette containing plant selection marker, hpt and gusa reporter gene of pcambia1301 binary vector in an elite indica rice cv. ir64. many fertile transgenic plants were generated using scutellar calli as initial explants through agrobacterium-m ... | 2006 | 16404581 |
| starch properties of mutant rice high in resistant starch. | as the staple food of over half the world's population, hot cooked rice high in resistant starch (rs) is of particular interest, which will have greater impact in the dietary prevention of diabetes and hyperlipidemia. a mutant rice high in rs in hot cooked rice, described as rs111, was comparatively studied with the wild type and common rice. despite obviously low rs content in the raw milled rice, the rs content in the hot cooked rice of mutant rs111 was significantly higher than that of the wi ... | 2006 | 16417315 |
| a tissue culture system for different germplasms of indica rice. | agrobacterium-mediated transformation of indica rice has been manipulated in only a limited number of cultivars because the majority of indica varieties are recalcitrant to in vitro response. establishment of a highly efficient and widely used tissue culture system for indica rice will accelerate the application of transformation technology in breeding programs and the study of the functions of indica-specific genes. by manipulating plant growth regulators, organic components and salts within th ... | 2006 | 16432631 |
| transcript abundance of rml1, encoding a putative gt1-like factor in rice, is up-regulated by magnaporthe grisea and down-regulated by light. | we isolated and sequenced both genomic dna and cdna clones, which encoded a putative gt1-like protein with 385 amino acids, from cultivated rice (oryza sativa ssp. indica). this protein shows significant amino acid sequence similarities with trihelix dna-binding gt-1a/b2f and gt-1 factors that were identified in dicot plants. northern blotting analysis indicated that the transcript of the rice gt-1 factor in seedling was up-regulated by the rice blast fungus magnaporthe grisea, down-regulated by ... | 2004 | 14693376 |
| analysis of the transcriptional response to rice yellow mottle virus infection in oryza sativa indica and japonica cultivars. | several cdna libraries were constructed using mrna isolated from roots, panicles, cell suspensions and leaves of non-stressed oryza sativa indica (ir64) and japonica (azucena) plants, from wounded leaves, and from leaves of both cultivars inoculated with rice yellow mottle virus (rymv). a total of 5549 cleaned expressed sequence tags (ests) were generated from these libraries. they were classified into functional categories on the basis of homology, and analyzed for redundancy within each librar ... | 2003 | 14564505 |
| inheritance of oryza sativa endornavirus in f1 and f2 hybrids between japonica and indica rice. | we have found a 14 kbp double-stranded rna (dsrna) in many cultivars of japonica rice (oryza sativa l.) but not in any cultivars of indica rice. this dsrna is an rna replicon with plasmid-like properties and is proposed to be a novel dsrna virus, oryza sativa endornavirus (osv). reciprocal crosses between the osv-carrier japonica variety (nipponbare) and the osv-free indica variety (ir 26 or kasalath) were performed to investigate whether osv can be transmitted to f1 hybrids. when ir 26 and nipp ... | 2003 | 12893964 |
| bioengineered 'golden' indica rice cultivars with beta-carotene metabolism in the endosperm with hygromycin and mannose selection systems. | vitamin-a deficiency (vad) is a major malnutrition problem in south asia, where indica rice is the staple food. indica-type rice varieties feed more than 2 billion people. hence, we introduced a combination of transgenes using the biolistic system of transformation enabling biosynthesis of provitamin a in the endosperm of several indica rice cultivars adapted to diverse ecosystems of different countries. the rice seed-specific glutelin promoter (gt-1 p) was used to drive the expression of phytoe ... | 2003 | 17147745 |
| transgenic indica rice resistant to sap-sucking insects. | agrobacterium-mediated genetic transformation has been optimized in indica rice susceptible to sap-sucking insects, viz., brown planthopper (bph) and green leafhopper (glh). snowdrop lectin gene (gna) from galanthus nivalis, driven by phloem-specific rice-sucrose-synthase promoter, along with herbicide resistance gene (bar) driven by camv 35s promoter, was employed for genetic transformation. embryogenic calli--after co-cultivation with agrobacterium strain lba4404 harbouring ti plasmid psb111-b ... | 2003 | 17156035 |
| marker-free transgenic (mft) near-isogenic introgression lines (niils) of 'golden' indica rice (cv. ir64) with accumulation of provitamin a in the endosperm tissue. | we have developed near-isogenic introgression lines (niils) of an elite indica rice cultivar (ir64) with the genes for beta-carotene biosynthesis from dihaploid (dh) derivatives of golden japonica rice (cv. t309). a careful analysis of the dh lines indicated the integration of the genes of interest [phytoene synthase (psy) and phytoene desaturase (crti)] and the selectable marker gene (hygromycin phosphotransferase, hph) in two unlinked loci. during subsequent crossing, progenies could be obtain ... | 2006 | 17177811 |
| air pollution and climate change both reduce indian rice harvests. | | 2006 | 17179041 |
| variable t-dna linkage configuration affects inheritance of carotenogenic transgenes and carotenoid accumulation in transgenic indica rice. | transgenics for the expression of beta-carotene biosynthetic pathway in the endosperm were developed in indica rice background by introducing phytoene synthase (psy) and phytoene desaturase (crti) genes through agrobacterium-mediated transformation, employing non-antibiotic positive selectable marker phosphomannose isomerase (pmi). twenty-seven transgenic lines were characterized for the structural organization of t-dna inserts and the expression of transgenes in terms of total carotenoid and be ... | 2007 | 17377795 |
| a set of multiplex panels of microsatellite markers for rapid molecular characterization of rice accessions. | this study aimed to analyze the efficiency of three new microsatellite multiplex panels, which were designed to evaluate a total of 16 loci of the rice genome, based on single pcr reactions of each panel. a sample of 548 accessions of traditional upland rice landraces collected in brazil in the last 25 years was genotyped, a database of allelic frequencies was established, estimates of genetic parameters were performed and analysis of genetic structure of the collection was developed. | 2007 | 17517133 |
| over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant avicennia marina in indica rice var pusa basmati-1 confers abiotic stress tolerance. | antioxidant enzymes play an important role in conferring abiotic stress tolerance. superoxide dismutase (sod) is the first enzyme in the enzymatic antioxidative pathway. halophytic plants like mangroves have been reported to have a high level of sod activity, which plays a major role in defending the mangrove species against severe abiotic stresses. we had previously reported the isolation of sod1, a cdna encoding a cytosolic copper zinc superoxide dismutase from the mangrove plant avicennia mar ... | 2008 | 17541718 |
| transgenic tobacco plants overexpressing the heterologous lea gene rab16a from rice during high salt and water deficit display enhanced tolerance to salinity stress. | the full length rab16a, from the indica rice pokkali, was introduced into tobacco by agrobacterium-mediated transformation. the transgene was stably integrated into the genome and they originated from different lines of integration. expression of rab16a transcript driven by its own promoter (stress inducible) in t2 progenies, only when triggered by salinity/aba/peg (polyethylene glycol)-mediated dehydration, but not at the constitutive level, led to the stress-induced accumulation of rab16a prot ... | 2007 | 17554543 |
| identification of quantitative trait loci associated with resistance to brown planthopper in the indica rice cultivar col.5 thailand. | the brown planthopper (bph) is one of the most serious insect pests of rice throughout asia. in this study, we constructed a linkage map to determine the locus for bph resistance gene, using an f(2) population from a cross between a resistant indica cultivar, 'col.5 thailand', and a susceptible cultivar '02428'. insect resistance was evaluated using 147 f(3) families and the genotype of each f(2) plant was inferred from the phenotype of corresponding f(3) families. two qtls was detected on chrom ... | 2007 | 17567441 |
| identification of five new blast resistance genes in the highly blast-resistant rice variety ir64 using a qtl mapping strategy. | rice progenies used for the construction of genetic maps permit exhaustive identification and characterization of resistance genes present in their parental cultivars. we inoculated a rice progeny derived from the cross ir64 x azucena with different magnaporthe grisea isolates that showed differential responses on the parental cultivars. by qtl mapping, nine unlinked loci conferring resistance to each isolate were identified and named pi-24( t) to pi-32( t). they could correspond to nine specifi ... | 2003 | 12647052 |
| promoters of two anther-specific genes confer organ-specific gene expression in a stage-specific manner in transgenic systems. | differential screening of a stage-specific cdna library of indica rice has been used to identify two genes expressed in pre-pollination stage panicles, namely osipa and osipk coding for proteins similar to expansins/pollen allergens and calcium-dependent protein kinases (cdpk), respectively. northern analysis and in situ hybridizations indicate that osipa expresses exclusively in pollen while osipk expresses in pollen as well as anther wall. promoters of these two anther-specific genes show the ... | 2007 | 17661051 |
| the expression pattern of a rice disease resistance gene xa3/xa26 is differentially regulated by the genetic backgrounds and developmental stages that influence its function. | genetic background and developmental stage influence the function of some disease resistance (r) genes. the molecular mechanisms of these modifications remain elusive. our results show that the two factors are associated with the expression of the r gene in rice xa3 (also known as xa26)-mediated resistance to xanthomonas oryzae pv. oryzae (xoo), which in turn influences the expression of defense-responsive genes. the background of japonica rice, one of the two major subspecies of asian cultivate ... | 2007 | 17720929 |
| genetic differentiation of wild relatives of rice as assessed by rflp analysis. | to study genetic diversity and relationships of wild relatives of rice, 58 accessions of oryza rufipogon, oryza nivara, oryza sativa f. spontanea and the cultivated oryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (rflp) technique. all 30-used rflp probes detected polymorphisms among the oryza accessions, with an average of 3.8 polymorphic fragments per probe. considerable genetic diversity was scored among the oryza ... | 2002 | 12582876 |
| characterization of a hkt-type transporter in rice as a general alkali cation transporter. | we report the characterization of rice oshkt1 (oryza sativa ssp. indica) homologous to the wheat k+/na+-symporter hkt1. expression of oshkt1 in the yeast strain cy162 defective in k+-uptake restored growth at mm and micro m concentrations of k+ and mediated hypersensitivity to na+. when expressed in xenopus oocytes, rice oshkt1 showed uptake characteristics of a na+-transporter but mediated transport of other alkali cations as well. oshkt1 expression was analysed in salt-tolerant rice pokkali an ... | 2002 | 12182709 |
| genetic variation of nbs-lrr class resistance genes in rice lines. | the use of plant disease resistance (r) genes in breeding programs needs an understanding of their variation patterns. in our current study, we investigated the polymorphisms of 44 nbs-lrr class r-genes among 21 rice cultivars and 14 wild rice populations. our data suggested that there were four basic types of variations: conserved, diversified, intermediate-diversified, and present/absent patterns. common characteristics at a locus of conserved r-genes were: copy-number uniformity, clear diverg ... | 2008 | 17932646 |
| comparison of nutritional quality between chinese indica rice with sck and cry1ac genes and its nontransgenic counterpart. | nutritional assessment of transgenic crops used for human food and animal feed is an important aspect of safety evaluations. an insect-resistant rice (irr) was generated by the stable insertion of sck, a modified cowpea trypsin inhibitor gene, and cry1ac, encoding a crystal protein from bacillus thuringiensis into the genome of a common variety of chinese indica rice. the composition of the brown and milled rice grain from the resulted irr line designated liangyou kefeng no. 6 was compared with ... | 2007 | 17995700 |
| two types of hkt transporters with different properties of na+ and k+ transport in oryza sativa. | it is thought that na+ and k+ homeostasis is crucial for salt-tolerance in plants. to better understand the na+ and k+ homeostasis in important crop rice (oryza sativa l.), a cdna homologous to the wheat hkt1 encoding k+-na+ symporter was isolated from japonica rice, cv nipponbare (ni-oshkt1). we also isolated two cdnas homologous to ni-oshkt1 from salt-tolerant indica rice, cv pokkali (po-oshkt1, po-oshkt2). the predicted amino acid sequence of ni-oshkt1 shares 100% identity with po-oshkt1 and ... | 2001 | 11489190 |
| [research on improving rice resistance to the pest by b.t. and sbti genes]. | b.t. gene alone or and sbti gene together were introduced into two elite indica rice varieties grown in south china by bombardment. and 21 independent transgenic lines containing b.t. gene and 4 independent transgenic lines containing b.t. gene and sbti gene were obtained. molecular and genetics analysis for r1 plants showed integration of multiple transgenes occurred at one genetic locus. northern blot result proved b.t. gene expressed in r2 transgenic plants stably. bioassays using r2 transgen ... | 2000 | 11191767 |
| [introduction of wide spectrum rice bacterial blight resistance gene xa21 into two-line genic male sterile rice variety pei'ai 64s]. | agrobacterium-mediated transformation of two-line genic male sterile indica rice variety pei'ai 64s was conducted using a cloned gene, xa21, as the foreign gene and mature embryo calli as the recipients. a total of 46 transgenic plants had been obtained. the pcr analysis and southern blotting showed the integration of xa21 gene into the genome the transgenic plants. results of inoculation with philippine race 6 of xanthomonas oryzae pv. oryzae indicated that most of transgenic plants obtained hi ... | 2000 | 10976312 |
| agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. | here, we provide comprehensive, highly efficient protocols for agrobacterium tumefaciens-mediated transformation of a wide range of rice genotypes. methods that use either immature embryos (japonica and indica rice) or calli (japonica cultivars and the indica cultivar, kasalath) as a starting material for inoculation with agrobacterium are described. immature embryos are pretreated with heat and centrifugal force, which significantly enhances the efficiency of gene transfer, and then infected wi ... | 2008 | 18451790 |
| haemoglobin (erythrogen)-enhanced post-thaw growth of cryopreserved cells. | supplementation of semi-solid r2 culture medium with a commercial bovine haemoglobin (hb) solution (erythrogen) at 1:50-1:500 (v:v), had beneficial effects on the growth, following cryopreservation, of cells of the indica rice, oryza sativa cv. pusa basmati 1. the mean absorbance, as assessed by triphenyl tetrazolium chloride reduction, of rice cells at 8 d post-thawing, was increased by up to 60% (p < 0.05), compared to cells recovered in the absence of hb. erythrogen (1:50-1:500 v:v) promoted ... | 1999 | 10092937 |
| expansion of a (ga) dinucleotide at a microsatellite locus associated with domestication in rice. | microsatellites undergo rapid changes over short evolutionary time periods which can be phylogenetically informative in related species. here we show the repeat unit expansion of a (ga)n-type microsatellite in the process of cultivation of rice from its wild ancestors. we amplified a microsatellite locus harboring (ga)n repeats from several wild and cultivated rices. sequencing revealed an increase in repeat number from 14 in distantly related wild rice species to 24 in the widely grown present- ... | 1998 | 9919358 |
| transgenic rice expressing allium sativum leaf agglutinin (asal) exhibits high-level resistance against major sap-sucking pests. | rice (oryza sativa) productivity is adversely impacted by numerous biotic and abiotic factors. an approximate 52% of the global production of rice is lost annually owing to the damage caused by biotic factors, of which approximately 21% is attributed to the attack of insect pests. in this paper we report the isolation, cloning and characterization of allium sativum leaf agglutinin (asal) gene, and its expression in elite indica rice cultivars using agrobacterium-mediated genetic transformation m ... | 2008 | 18854007 |
| origin, dispersal, cultivation and variation of rice. | there are two cultivated and twenty-one wild species of genus oryza. o. sativa, the asian cultivated rice is grown all over the world. the african cultivated rice, o. glaberrima is grown on a small scale in west africa. the genus oryza probably originated about 130 million years ago in gondwanaland and different species got distributed into different continents with the breakup of gondwanaland. the cultivated species originated from a common ancestor with aa genome. perennial and annual ancestor ... | 1997 | 9291957 |
| transgenic elite indica rice plants expressing cryiac delta-endotoxin of bacillus thuringiensis are resistant against yellow stem borer (scirpophaga incertulas). | generation of insect-resistant, transgenic crop plants by expression of the insecticidal crystal protein (icp) gene of bacillus thuringiensis (bt) is a standard crop improvement approach. in such cases, adequate expression of the most appropriate icp against the target insect pest of the crop species is desirable. it is also considered advantageous to generate bt-transgenics with multiple toxin systems to control rapid development of pest resistance to the icp. larvae of yellow stem borer (ysb), ... | 1997 | 9122157 |
| rice scutellum induces agrobacterium tumefaciens vir genes and t-strand generation. | for successful transformation of a plant by agrobacterium tumefaciens it is essential that the explant used in cocultivation has the ability to induce agrobacterium tumour-inducing (ti) plasmid virulence (vir) genes. here we report a significant variation in different tissues of indica rice (oryza sativa l. cv. co43) in their ability to induce agrobacterium tumefaciens vir genes and t-strand generation, using explants preincubated in liquid murashige and skoog (ms) medium. an analysis of rice le ... | 1995 | 7579158 |
| persistence of endrin in indian rice soils under flooded conditions. | | 1976 | 956537 |
| genetic diversity and origin of weedy rice (oryza sativa f. spontanea) populations found in north-eastern china revealed by simple sequence repeat (ssr) markers. | weedy rice (oryza sativa f. spontanea) is one of the most notorious weeds occurring in rice-planting areas worldwide. the objectives of this study are to determine the genetic diversity and differentiation of weedy rice populations from liaoning province in north-eastern china and to explore the possible origin of these weedy populations by comparing their genetic relationships with rice varieties (o. sativa) and wild rice (o. rufipogon) from different sources. | 2006 | 17056615 |
| assessment of gene flow from a herbicide-resistant indica rice (oryza sativa l.) to the costa rican weedy rice (oryza sativa) in tropical america: factors affecting hybridization rates and characterization of f1 hybrids. | herbicide-resistant rice cultivars allow selective weed control. a glufosinate indica rice has been developed locally. however, there is concern about weedy rice becoming herbicide resistant through gene flow. therefore, assessment of gene flow from indica rice cultivars to weedy rice is crucial in tropical america. a field trial mimicking crop-weed growing patterns was established to assess the rate of hybridization between a costa rican glufosinate-resistant rice line (ppt-r) and 58 weedy rice ... | 2009 | 19330532 |
| indica rice (oryza sativa, br29 and ir64). | rice is the world's most important food crop. indica-type rice provides the staple food for more than half of the world population. to satisfy the growing demand of the ever-increasing population, more sustained production of indica-type rice is needed. in addition, because of the high per capita consumption of indica rice, improvement of any traits including its nutritive value may have a significant positive health outcome for the rice-consuming population. rice yield productivity is greatly a ... | 2006 | 16988345 |
| phylogeography of asian wild rice, oryza rufipogon, reveals multiple independent domestications of cultivated rice, oryza sativa. | cultivated rice, oryza sativa l., represents the world's most important staple food crop, feeding more than half of the human population. despite this essential role in world agriculture, the history of cultivated rice's domestication from its wild ancestor, oryza rufipogon, remains unclear. in this study, dna sequence variation in three gene regions is examined in a phylogeographic approach to investigate the domestication of cultivated rice. results indicate that india and indochina may repres ... | 2006 | 16766658 |
| haplotype variation in structure and expression of a gene cluster associated with a quantitative trait locus for improved yield in rice. | by constructing nearly isogenic lines (nils) that differ only at a single quantitative trait locus (qtl), we fine-mapped the yield-improving qtl qgy2-1 to a 102.9-kb region on rice chromosome 2. comparison analysis of the genomic sequences in the mapped qtl region between the donor (dongxiang wild rice, oryza rufipogon griff.) and recurrent (guichao2, oryza sativa ssp. indica) parents used for the development of nils identified the haplotypes of a leucine-rich repeat receptor kinase gene cluster ... | 2006 | 16606701 |
| glycinebetaine-induced water-stress tolerance in coda-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes. | rice (oryza sativa l.), a non-accumulator of glycinebetaine (gb), is highly susceptible to abiotic stress. transgenic rice with chloroplast-targeted choline oxidase encoded by the coda gene from arthrobacter globiformis has been evaluated for inheritance of transgene up to r5 generation and water-stress tolerance. during seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem ii and they show better physiological performance, for example, enh ... | 2009 | 19490479 |
| molecular characterization of marker-free transgenic lines of indica rice that accumulate carotenoids in seed endosperm. | a single agrobacterium strain harbouring two binary plasmids was successfully used for the first time to develop a marker-free transgenic rice of improved nutritional value. sixty-eight t0 co-transformants were obtained in three indica rice cultivars--two popular high-yielding bangladeshi varieties (br28 and br29), and one high-iron rice cultivar (ir68144). marker-free lines were obtained from 14 out of 24 selected co-transformants screened in the t1 generation. the accumulation of total caroten ... | 2005 | 16179991 |
| phylogenetic relationships among a-genome species of the genus oryza revealed by intron sequences of four nuclear genes. | the a-genome group in oryza consists of eight diploid species and is distributed world-wide. here we reconstructed the phylogeny among the a-genome species based on sequences of nuclear genes and mite (miniature inverted-repeat transposable elements) insertions. thirty-seven accessions representing two cultivated and six wild species from the a-genome group were sampled. introns of four nuclear single-copy genes on different chromosomes were sequenced and analysed by both maximum parsimony (mp) ... | 2005 | 15948847 |
| systematic identification of x1-homologous genes reveals a family involved in stress responses in rice. | x1-homologous genes (xhs) encode plant-specific proteins containing three major domains (xh, xs, zf-xs), but their functions are largely unknown. we report the systematic identification and characterization of xhs genes in the rice genome. eleven putative xhs protein sequences (oxhs1-11) were identified in the sequenced genome of oryza sativa japonica cv. nipponbare, and these sequences, along with other plant xhs homologues, were classified into five subgroups based on phylogenetic analysis. di ... | 2009 | 19701685 |
| [determination of amylose content in transgenic progenies containing an antisense waxy gene and identifying of pure lines in indica rice]. | through genetic transformation mediated by agrobacterium tumefaciens, an antisense waxy gene was introduced into longtefu b line, a male sterile maintainer line in indica (oryza sativa l.). thirty transgenic plants showed integration of antisense waxy gene into the genome as determined by pcr assay, and twenty eight were confirmed by southern blotting. t1 seeds from twenty one transgenic plants showed a marked decrease of amylose content, ranging from 3%-13% less than control, and seeds from som ... | 2004 | 15789760 |
| [physiological and genetic analysis of lesion resembling disease mutants (lrd) of oryza sativa l]. | ten indica rice and eight japonica rice mutants with lesion resembling disease (lrd27-44) were obtained by gamma-ray mutagenesis of the whole genomes. these mutants exhibited diverse lesion mimic phenotypes under different growth environments, could be accordingly classified two types, sensitive and insensitive to environments. basing on difference in development of lesion mimics, they can be divided into three categories: whole life lesion mimics (wllm), vegetative initiation lesion mimics (vil ... | 2004 | 15599031 |
| effective strategy for pyramiding three bacterial blight resistance genes into fine grain rice cultivar, samba mahsuri, using sequence tagged site markers. | bacterial leaf blight (bb) of rice is a major disease limiting rice production in several rice growing regions of the world. the pathogen, xanthomonas oryzae pv oryzae, causing the disease is highly virulent to rice crops and is capable of evolving new races. breeding efforts to incorporate single bb resistant gene often leads to resistance breakdown within a short period. to overcome such breakdown of resistance and develop germplasm with durable disease resistance, we have introgressed three b ... | 2010 | 20349335 |
| molecular characterization of a light-responsive gene, breast basic conserved protein 1 (osibbc1), encoding nuclear-localized protein homologous to ribosomal protein l13 from oryza sativa indica. | rice (oryza sativa l. subsp. indica) cdna for the gene osibbc1, encoding homologue of the breast basic conserved protein 1 (bbc1), similar to ribosomal protein l13, has been identified and characterized. osibbc1 codes for a 24 kda highly basic protein with two potential bipartite nuclear localization signals (nls) and a transcriptional activation domain (tad). the structural part of the gene is interrupted by four introns. the osibbc1 gene is represented by two copies in the rice genome and both ... | 2004 | 14746913 |
| proteome analysis of cultivar-specific deregulations of oryza sativa indica and o. sativa japonica cellular suspensions undergoing rice yellow mottle virus infection. | we have used two-dimensional gel electrophoresis with mass spectrometry analysis to study the temporal patterns of protein expression during rymv (rice yellow mottle virus) infection in rice cells of two cultivars: ir64, oryza sativa indica, susceptible, and azucena, o. sativa japonica, partially resistant to rymv. proteomic analysis of nonstressed and rymv inoculated cells showed statistically significant changes in the relative levels of 40 ir64 proteins and 24 azucena proteins. protein identi ... | 2004 | 14730683 |
| overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic arabidopsis plants. | dna topoisomerase 6 (top6) belongs to a novel family of type ii dna topoisomerases present, other than in archaebacteria, only in plants. here we report the isolation of full-length cdnas encoding putative top6 subunits a and b from rice (oryza sativa ssp. indica), preserving all the structural domains conserved among archaebacterial top6 homologs and eukaryotic meiotic recombination factor spo11. ostop6a1 was predominantly expressed in prepollinated flowers. the transcript abundance of ostop6a2 ... | 2006 | 17116242 |
| mature seed-derived callus of the model indica rice variety kasalath is highly competent in agrobacterium-mediated transformation. | we previously established an efficient agrobacterium-mediated transformation system using primary calli derived from mature seeds of the model japonica rice variety nipponbare. we expected that the shortened tissue culture period would reduce callus browning--a common problem with the indica transformation system during prolonged tissue culture in the undifferentiated state. in this study, we successfully applied our efficient transformation system to kasalath--a model variety of indica rice. th ... | 2010 | 20853107 |
| development of genotype-independent regeneration system for transformation of rice (oryza sativa ssp. indica). | rice (oryza sativa ssp. indica) is an important economic crop in many countries. although a variety of conventional methods have been developed to improve this plant, manipulation by genetic engineering is still complicated. we have established a system of multiple shoot regeneration from rice shoot apical meristem. by use of ms medium containing 4 mg l(-1) thidiazuron (tdz) multiple shoots were successfully developed directly from the meristem without an intervening callus stage. all rice culti ... | 2007 | 17139419 |
| regeneration of transgenic plants from two indica rice (oryza sativa l.) cultivars using shoot apex explants. | we have established a reproducible procedure for transformation of shoot apices and regeneration of transgenic plants for two indica rice cultivars, white ponni (wp) and pusa basmathi 1 (pb 1). four-day-old shoot apex explants were transformed by cocultivation with agrobacterium tumefaciens strain eha 101 harbouring a binary plasmid prit1. the vector contained an improved hygromycin phosphotransferase (hpt) gene for hygromycin resistance driven by actin 1 promoter and the reporter gene beta-gluc ... | 2007 | 17593368 |
| transcriptional profiling of indica rice cultivar iet8585 (ajaya) infected with bacterial leaf blight pathogen xanthomonas oryzae pv oryzae. | an indica rice cultivar iet8585 (ajaya) resists diverse races of the xanthomonas oryzae pv oryzae pathogen attack, and is often cultivated as bacterial leaf blight (blb) resistant check in india. earlier we reported a recessive blb resistance gene mapped to the long arm of chromosome 5 in iet8585. recessive gene-mediated blb resistance mechanism is not yet clearly understood. here we analyzed the transcriptional profile of the blb infected resistant cultivar by rice 22k oligo array. microarray a ... | 2007 | 17870590 |
| tac1, a major quantitative trait locus controlling tiller angle in rice. | a critical step during rice (oryza sativa) cultivation is dense planting: a wider tiller angle will increase leaf shade and decrease photosynthesis efficiency, whereas a narrower tiller angle makes for more efficient plant architecture. the molecular basis of tiller angle remains unknown. this research demonstrates that tiller angle is controlled by a major quantitative trait locus, tac1 (tiller angle control 1). tac1 was mapped to a 35-kb region on chromosome 9 using a large f(2) population fro ... | 2007 | 17908158 |
| molecular characterization and the effect of salinity on cyanobacterial diversity in the rice fields of eastern uttar pradesh, india. | salinity is known to affect almost half of the world's irrigated lands, especially rice fields. furthermore, cyanobacteria, one of the critical inhabitants of rice fields have been characterized at molecular level from many different geographical locations. this study, for the first time, has examined the molecular diversity of cyanobacteria inhabiting indian rice fields which experience various levels of salinity. | 2009 | 19344531 |
| transgenic expression of coat protein gene of rice tungro bacilliform virus in rice reduces the accumulation of viral dna in inoculated plants. | rice tungro, a devastating disease of rice in south and southeast asia, is caused by the joint infection of rice tungro bacilliform virus (rtbv) and rice tungro spherical virus (rtsv). in order to obtain transgenic resistance against rtbv, indica rice cultivar pusa basmati-1 was transformed to express the coat protein (cp) gene of an indian isolate of rtbv. rice plants containing the transgene integrated in low copy numbers were obtained, in which the cp was shown to accumulate in the leaf tissu ... | 2009 | 19387813 |
| piercing and vacuum infiltration of the mature embryo: a simplified method for agrobacterium-mediated transformation of indica rice. | traditional transformation methods are complex and time consuming. it is generally difficult to transform indica rice varieties using traditional transformation methods due to their poor regeneration. in this contribution, a simple method was developed for the transformation of indica rice. in this method, the mature embryos of soaked seeds were pierced by a needle, and then soaked in the agrobacterium inoculum under vacuum infiltration. the inoculated seeds germinated and grew to maturation (t ... | 2009 | 19455339 |
| subspecies-specific intron length polymorphism markers reveal clear genetic differentiation in common wild rice (oryza rufipogon l.) in relation to the domestication of cultivated rice (o. sativa l.). | it is generally accepted that oryza rufipogon is the progenitor of asian cultivated rice (o. sativa). however, how the two subspecies of o. sativa (indica and japonica) were domesticated has long been debated. to investigate the genetic differentiation in o. rufipogon in relation to the domestication of o. sativa, we developed 57 subspecies-specific intron length polymorphism (ssilp) markers by comparison between 10 indica cultivars and 10 japonica cultivars and defined a standard indica rice an ... | 2009 | 19631918 |
| selection on grain shattering genes and rates of rice domestication. | molecular cloning of major quantitative trait loci (qtls) responsible for the reduction of rice grain shattering, a hallmark of cereal domestication, provided opportunities for in-depth investigation of domestication processes. here, we studied nucleotide variation at the shattering loci, sh4 and qsh1, for cultivated rice, oryza sativa ssp. indica and oryza sativa ssp. japonica, and the wild progenitors, oryza nivara andoryza rufipogon. the nonshattering sh4 allele was fixed in all rice cultivar ... | 2009 | 19674325 |
| the antioxidant and antiproliferative activities of methanolic extracts from njavara rice bran. | free radical-induced oxidative stress is the root cause for many human diseases. naturally occurring antioxidant supplements from plants are vital to counter the oxidative damage in cells. the main objective of the present study was to characterize the antioxidant and antiproliferative potential of rice bran extracted from an important indian rice variety, njavara and to compare the same with two commercially available basmati rice varieties: vasumathi, yamini and a non medicinal variety, jyothi ... | 2010 | 20109194 |
| indian rice "kasalath" contains genes that improve traits of japanese premium rice "koshihikari". | rice (oryza sativa l.) chromosome segment substitution lines (cssls), in which chromosomal segments of the indian landrace "kasalath" replace the corresponding endogenous segments in the genome of the japanese premium rice "koshihikari", are available and together cover the entire genome. chromosome regions affecting a trait (crats) can be identified by comparison of phenotypes with genotypes of cssls. we detected 99 crats for 15 agronomic or morphological traits. "kasalath" had positively actin ... | 2008 | 18097643 |
| interspecific rice hybrid of oryza sativa x oryza nivara reveals a significant increase in seed protein content. | wild species offer a potential reservoir of genetic variation for crop improvement. besides the valuable genes for disease resistance that the wild species have provided for rice improvement, recent studies have shown that these wild species could also provide favorable alleles for the improvement of yield and yield-related traits. the present study reports yet another potential of wild relatives of rice, which involves the improvement of seed protein content. a significant increase in seed prot ... | 2008 | 18163552 |
| rice yellow mottle virus stress responsive genes from susceptible and tolerant rice genotypes. | the effects of viral infection involve concomitant plant gene variations and cellular changes. a simple system is required to assess the complexity of host responses to viral infection. the genome of the rice yellow mottle virus (rymv) is a single-stranded rna with a simple organisation. it is the most well-known monocotyledon virus model. several studies on its biology, structure and phylogeography have provided a suitable background for further genetic studies. 12 rice chromosome sequences are ... | 2008 | 18315879 |
| extraordinarily polymorphic ribosomal dna in wild and cultivated rice. | a collection of 481 rice accessions was surveyed for ribosomal dna (rdna) intergenic spacer length polymorphism to assess the extent of genetic diversity in chinese and asian rice germplasm. the materials included 83 accessions of common wild rice, oryza rufipogon, 75 of which were from china; 348 entries of cultivated rice (oryza sativa), representing almost all the rice growing areas in china; and 50 cultivars from south and east asia. a total of 42 spacer length variants (slvs) were detected. ... | 1996 | 18469959 |
| rice dreb1b promoter shows distinct stress-specific responses, and the overexpression of cdna in tobacco confers improved abiotic and biotic stress tolerance. | cbf/dreb (c-repeat binding factor/dehydration responsive element binding factor) family of transcription factors in plants is reported to be associated with regulation of gene expression under stress conditions. here, we report the functional characterization of a dreb transcription factor, dreb1b gene from rice (oryza sativa ssp. indica). the osdreb1b gene was differentially regulated at the transcriptional level by osmotic stress, oxidative stress, salicylic acid, aba, and cold. a 745 bp promo ... | 2008 | 18754079 |
| effect of supplementation with tamarind and chilli on the growth of young rats on a poor-south-indian-rice diet. | | 1948 | 18933125 |
| chymotrypsin protease inhibitor gene family in rice: genomic organization and evidence for the presence of a bidirectional promoter shared between two chymotrypsin protease inhibitor genes. | protease inhibitors play important roles in stress and developmental responses of plants. rice genome contains 17 putative members in chymotrypsin protease inhibitor (ranging in size from 7.21 to 11.9 kda) gene family with different predicted localization sites. full-length cdna encoding for a putative subtilisin-chymotrypsin protease inhibitor (ocpi2) was obtained from pusa basmati 1 (indica) rice seedlings. 620 bp-long ocpi2 cdna contained 219 bp-long orf, coding for 72 amino acid-long 7.7 kda ... | 2009 | 18952157 |
| characterization of the genome expression trends in the heading-stage panicle of six rice lineages. | to study how changes in gene regulation shape phenotypic variations in rice, we performed a comparative analysis of genome expression in the heading-stage panicle from six lineages of cultivated and wild rice, including oryza sativa subsp. indica, japonica and javanica, o. nivara , o. rufipogon and o. glaberrima. while nearly three-quarters of the genes are expressed at a constant level in all six lineages, a large portion of the genome, ranging from 1767 to 4489 genes, exhibited differential ex ... | 2009 | 18996467 |
| identification of ozone stress in indian rice through foliar injury and differential protein profile. | the present study showed a possibility to use phenotypic and proteomic responses in rice plants as an in vivo biomarker to detect higher concentrations of ambient ozone (o(3)). the investigation was done on two cultivars of indian rice using open top chambers ventilated with charcoal filtered air, ambient air, ambient air with 10 ppb o(3) exposure and ambient air with 20 ppb o(3) exposure at a rural site of varanasi, india. results showed that the magnitude of o(3) induced specific type of folia ... | 2010 | 19184480 |
| efficient in vitro plant regeneration via leaf base segments of indica rice (oryza sativa l.). | a reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (oryza sativa) cultivar rasi. the effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-d on callus induction frequency was investigated with a future aim to use leaf bases for biolistic and agrobacterium-mediated transformation experiments. friable, nodular and white to p ... | 2009 | 19317355 |
| rice root-associated bacteria: insights into community structures across 10 cultivars. | in this study, the effects of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated. thus, total bacteria, alpha- and betaproteobacteria, pseudomonas and actinobacteria were studied using pcr, followed by denaturing gradient gel electrophoresis (pcr-dgge). rice genotype determined, to a large extent, the composition of the different bacterial communities across cultivars. several cultivars belongin ... | 2011 | 21426364 |
| genetic dissection of the resistance to rice stripe virus present in the indica rice cultivar 'ir24' | rice stripe disease, caused by rice stripe virus (rsv) and transmitted by the small brown planthopper (laodelphax striatellus fallen), is one of the most serious viral diseases of rice in temperate east asian production regions. prior quantitative trait loci (qtl) mapping has established that oryza sativa l. subsp. indica 'ir24' carries positive alleles at the three loci qstv3, qstv7, and qstv11-i. here, we report an advanced backcross analysis based on three selected chromosome segment substitu ... | 2011 | 21793697 |
| agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice (oryza sativa l. sub sp. indica cv. adt 43). | a simple and efficient protocol for the agrobacterium-mediated transformation of an agronomically useful abiotic sensitive popular indica rice cv. adt 43 has been developed. initiation of calli were best achieved from the leaf bases of 4 days old rice seedlings on ls medium supplemented with 2.5mg/l 2,4-d and 1.0mg/l thiamine-hcl. rice calli immersed in agrobacterium suspension (strain eha 105, od(600)=0.8) were co-cultured on ls30-aspc medium for 2 days at 25±2°c in the dark. based on gus expre ... | 2011 | 21763536 |
| polymorphisms and evolutionary history of retrotransposon insertions in rice promoters. | retrotransposons are ubiquitous in higher plant genomes. the presence or absence of retrotransposons in whole genome and high throughput genomic sequence (htgs) from cultivated and wild rice was investigated to understand the organization and evolution of retrotransposon insertions in promoter regions. approximately half of the oryza sativa subsp. japonica 'nipponbare' promoters with retrotransposons conserved in oryza sativa subsp. indica '93-11' and four wild rice species showed higher sequenc ... | 2011 | 21823826 |
| characterization of a pollen-preferential gene osiagp from rice (oryza sativa l. subspecies indica) coding for an arabinogalactan protein homologue, and analysis of its promoter activity during pollen development and pollen tube growth. | during differential screening of inflorescence-specific cdna libraries from oryza sativa indica, an arabinogalactan protein (osiagp) cdna (586 bp) expressing preferentially in the inflorescence has been isolated. it encodes an arabinogalactan protein of 59 amino acids (6.4 kda) with a transmembrane domain and a secretory domain at the n terminus. the protein shows homology with agp23 from arabidopsis, and its homologue in japonica rice is located on chromosome 6. osiagp transcripts also accumula ... | 2010 | 19771527 |
| High-amylose rice improves indices of animal health in normal and diabetic rats. | A high-amylose rice with 64.8% amylose content (AC) was developed by transgenic inhibition of two isoforms of starch branching enzyme (SBE), SBEI and SBEIIb, in an indica rice cultivar. The expression of SBEI and SBEIIb was completely inhibited in the transgenic line, whereas the expression of granule-bound starch synthase was normal. Compared with wild-type rice, drastic reductions in both SBEs in the transgenic rice increased apparent AC in flour from 27.2% to 64.8%, resistant starch (RS) cont ... | 2011 | 22145600 |
| arsenic affects mineral nutrients in grains of various indian rice (oryza sativa l.) genotypes grown on arsenic-contaminated soils of west bengal. | the exposure of paddy fields to arsenic (as) through groundwater irrigation is a serious concern that may not only lead to as accumulation to unacceptable levels but also interfere with mineral nutrients in rice grains. in the present field study, profiling of the mineral nutrients (iron (fe), phosphorous, zinc, and selenium (se)) was done in various rice genotypes with respect to as accumulation. a significant genotypic variation was observed in elemental retention on root fe plaque and their a ... | 2010 | 20490609 |
| generation of marker-free bt transgenic indica rice and evaluation of its yellow stem borer resistance. | we report on generation of marker-free (‘clean dna’) transgenic rice (oryza sativa), carrying minimal gene-expression-cassettes of the genes of interest, and evaluation of its resistance to yellow stem borer scirpophaga incertulas (lepidoptera: pyralidae). the transgenic indica rice harbours a translational fusion of 2 different bacillus thuringiensis (bt) genes, namely cry1b-1aa, driven by the green-tissue-specific phosphoenol pyruvate carboxylase (pepc) promoter. mature seed-derive ... | 2010 | 20720299 |
| pollen-specific expression of oryza sativa indica pollen allergen gene (osipa) promoter in rice and arabidopsis transgenic systems. | earlier, a pollen-specific oryza sativa indica pollen allergen gene (osipa), coding for expansins/pollen allergens, was isolated from rice, and its promoter--upon expression in tobacco and arabidopsis--was found active during the late stages of pollen development. in this investigation, to analyze the effects of different putative regulatory motifs of osipa promoter, a series of 5' deletions were fused to β-glucuronidase gene (gus) which were stably introduced into rice and arabidopsis. histoche ... | 2011 | 21061188 |