Publications

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desulforhabdus amnigenus gen. nov. sp. nov., a sulfate reducer isolated from anaerobic granular sludge.from granular sludge of an upflow anaerobic sludge bed (uasb) reactor treating paper-mill wastewater, a sulfate-reducing bacterium (strain asrb1) was isolated with acetate as sole carbon and energy source. the bacterium was rod-shaped, (1.4-1.9 x 2.5-3.4 microns), nonmotile, and gram-negative. optimum growth with acetate occurred around 37 degrees c in freshwater medium (doubling time: 3.5-5.0 days). the bacterium grew on a range of organic acids, such as acetate, propionate, and butyrate, and o ...19958588734
kinetics of acetate oxidation by two sulfate reducers isolated from anaerobic granular sludgekinetic parameters of acetate oxidation were determined for the sulfate reducers desulforhabdus amnigenus and desulfobacca acetoxidans. based on these parameters, both sulfate reducers seem to be able to outcompete methanosaeta spp. for acetate in acetate-fed anaerobic bioreactors. mixed-substrate studies showed that d. amnigenus degraded acetate and hydrogen simultaneously but preferred lactate, propionate, and ethanol over acetate.19989603856
sulfate-reducing bacteria in rice field soil and on rice roots.rice plants that were grown in flooded rice soil microcosms were examined for their ability to exhibit sulfate reducing activity. washed excised rice roots showed sulfate reduction potential when incubated in anaerobic medium indicating the presence of sulfate-reducing bacteria. rice plants, that were incubated in a double-chamber (phylloshpere and rhizosphere separated), showed potential sulfate reduction rates in the anoxic rhizosphere compartment. these rates decreased when oxygen was allowed ...199910390878
desulfovirga adipica gen. nov., sp. nov., an adipate-degrading, gram-negative, sulfate-reducing bacterium.a novel, mesophilic, gram-negative bacterium was isolated from an anaerobic digestor for municipal wastewater. the bacterium degraded adipate in the presence of sulfate, sulfite, thiosulfate and elemental sulfur. (e)-2-hexenedioate accumulated transiently in the degradation of adipate. (e)-2-hexenedioate, (e)-3-hexenedioate, pyruvate, lactate, c1-c12 straight-chain fatty acids and c2-c10 straight-chain primary alcohols were also utilized as electron donors. 3-phenylpropionate was oxidized to ben ...200010758870
structure and diversity of gram-negative sulfate-reducing bacteria on rice roots.specific pcr assays were used to amplify the 16s rrna genes of the desulfobacteriaceae and the desulfovibrionaceae from extracted environmental dna from rice roots. 16s rdna-based community patterns of the desulfobacteriaceae were generated via terminal restriction fragment length polymorphism analysis from rice roots and compared with bulk soil. the molecular fingerprints showed no significant difference between rice roots and bulk soil, but changes during the vegetation period. 16s rdna clone ...200111451522
monoalkylether phospholipids in the sulfate-reducing bacteria desulfosarcina variabilis and desulforhabdus amnigenus.in this study, cellular lipid compositions of two mesophilic sulfate-reducing bacteria were analyzed by high performance liquid chromatography-mass spectrometry (hplc-ms). in desulfosarcina variabilis and desulforhabdus amnigenus, alkylether-containing phospholipids were detected which had previously only been found in significant amounts in deeply branching hyperthermophilic bacteria and archaea. combining information from hplc-ms analysis and chemical degradation experiments, ether lipids were ...200111734887
molecular characterization of sulfate-reducing bacteria in the guaymas basin.the guaymas basin (gulf of california) is a hydrothermal vent site where thermal alteration of deposited planktonic and terrestrial organic matter forms petroliferous material which supports diverse sulfate-reducing bacteria. we explored the phylogenetic and functional diversity of the sulfate-reducing bacteria by characterizing pcr-amplified dissimilatory sulfite reductase (dsrab) and 16s rrna genes from the upper 4 cm of the guaymas sediment. the dsrab sequences revealed that there was a major ...200312732547
quantification of gram-negative sulphate-reducing bacteria in rice field soil by 16s rrna gene-targeted real-time pcr.for the quantification of gram-negative sulphate reducers in rice fields, 11 real-time pcr assays were established targeting 16s rrna genes combined with sybrgreen detection. three of these assays were specific for the "main" groups, i.e. the desulfovibrionaceae, the desulfobacteraceae and desulfobulbus sp., whereas eight assays were developed for subgroups within the first two main groups. the detection limits of the assays were between 2 x 10(5) and 4 x 10(3) targets g(-1) (wet weight) or less ...200415063062
simple organic electron donors support diverse sulfate-reducing communities in fluidized-bed reactors treating acidic metal- and sulfate-containing wastewater.bacterial diversity of lactate- and ethanol-utilizing sulfate-reducing fluidized-bed reactor (fbr) communities was investigated with culture-independent methods. the fbrs were fed for 500 days with synthetic mineral processing wastewater containing sulfate, zinc and iron with hydraulic retention time of 16-24 h. sodium lactate or ethanol was used as electron donor for microbial sulfate reduction. for microbial characterization, 16s rrna gene clone libraries and denaturing gradient gel electropho ...200419712316
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