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restriction site mapping of a bovine adenovirus type 10 strain.physical maps of the genome of a bovine adenovirus (bav) type 10 strain (isolate belfast1) were constructed for eight restriction enzymes. the size of the viral genome was estimated to be around 29,800 base pairs (bp). the orientation of the maps was determined on the basis of partial dna sequences of the cloned psti/d fragment of belfast1. this work is an introduction to dna sequence and phylogenetic analysis of bav-10 in order to clarify its taxonomic place.19969055463
sequencing and phylogenetic analysis of the protease gene, and genetic mapping of bovine adenovirus type 10 define its relatedness to other bovine adenoviruses.the complete genome of a bovine adenovirus (bav) type 10 isolate was molecularly cloned and partially sequenced. the encoded proteins were predicted by computer analysis of the dna sequences of the ends or the entire length of the cloned viral fragments, and thus a rough genetic map was constructed. the protease gene of bav-10 was completely sequenced and used in phylogenetic analysis. based on the results of the phylogenetic analysis, and the location and presence of certain genes thought to be ...19989712509
examination of adenovirus-types in intestinal vascular endothelial inclusions in fatal cases of enteric disease in cattle, by in situ hybridisation.there have been occasional reports in the literature of a severe naturally occurring enteric disease of cattle in which adenoviral inclusions were found in intestinal vascular endothelium. bovine adenovirus type 10 (bav-10) was identified by in situ hybridisation (ish) in the inclusions of all 13 such cattle detected in northern ireland [smyth, j.a., benkö, m., moffett, d.a., harrach, b., 1996. j. clin. microbiol. 34, 1270-1274]. the present paper describes ish examination of the vascular endoth ...199910591492
dna sequencing and analysis of the right-hand part of the genome of the unique bovine adenovirus type 10.the prototype strain of bovine adenovirus (badv) type 10 and four additional isolates that were indistinguishable in serum-neutralization tests have been shown to have remarkable variation in their genome size and restriction maps. in the present study, more than 40 % of the dna sequence of the badv-10 isolate with the longest genome was determined. a biased base composition resulting in low (<41 %) gc content was noticed. analysis of the genes of the dna-binding protein, 100k, 33k, pviii and fi ...200414993642
bovine adenovirus type 10: properties of viruses isolated from cases of bovine haemorrhagic enterocolitis.the isolation, serological classification and growth properties of adenoviruses isolated from fatal cases of haemorrhagic enterocolitis in calves are described. four viruses, from different submissions, were isolated in cultures of calf testis cells and were identified as adenoviruses by electron microscopy. the four isolates were serologically identical and were classified as bovine adenovirus type 10 in cross-neutralisation tests with other bovine, ovine and porcine adenovirus species. their g ...19968734506
bovine adenovirus type 10 identified in fatal cases of adenovirus-associated enteric disease in cattle by in situ hybridization.a severe, naturally occurring enteric disease of cattle in which adenovirus inclusions are present in the intestinal vascular endothelium has been recognized in several countries; three different adenovirus serotypes have been isolated from affected animals. an in situ hybridization technique for the detection of bovine adenoviral dna was developed and applied to tissue from 13 cattle in northern ireland diagnosed to have the disease. bovine adenovirus serotype 10 (bav-10) was identified in the ...19968727916
clinicopathological features of 11 suspected outbreaks of bovine adenovirus infection and development of a real-time quantitative pcr to detect bovine adenovirus type 10.a retrospective study was conducted to investigate 11 outbreaks of presumptive fatal adenovirus infection diagnosed through two new zealand diagnostic laboratories during 2014 and 2015. outbreaks occurred in 6-12-month-old friesian or friesian cross cattle during autumn, winter and spring. individual outbreaks were short in duration, with mortality rates ranging from 3/250 to 20/600 (1.2 to 3.3%).201627277320
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