| intra-chromosomal heterogeneity between the four 16s rrna gene copies in the genus veillonella: implications for phylogeny and taxonomy. | among the seven species characterized within the genus veillonella, three (veillonella dispar, veillonella parvula and veillonella atypica) have so far been isolated from human flora and during infectious processes. sequencing and analysis of 16s rdna (rrs) has been described as the best method for identification of veillonella strains at the species level since phenotypic characteristics are unable to differentiate between species. rrs sequencing for the three species isolated from humans showe ... | 2003 | 12777489 |
| mass transport of macromolecules within an in vitro model of supragingival plaque. | the aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. polyspecies biofilms containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, streptococcus sobrinus, veillonella dispar, and candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. subsequent examinatio ... | 2003 | 12620862 |
| selection of dairy bacterial strains as probiotics for oral health. | the aim of the present study was to select bacterial strains with potential properties as oral probiotics, namely for the prevention of dental caries. we examined 23 dairy microorganisms, out of which we identified two streptococcus thermophilus and two lactcoccus lactis strains that were able to adhere to saliva-coated hydroxyapatite beads to the same extent as streptococcus sobrinus omz176. two of them, strep. thermophilus ncc1561 and lactoc. lactis ssp. lactis ncc2211, were further successful ... | 2002 | 12120707 |
| effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms. | antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ... | 2002 | 12037363 |
| prosthetic joint infection due to veillonella dispar. | described here is the first report of a monomicrobial joint infection caused by veillonella dispar that resulted in loosening of a prosthesis. this clinical case shows that veillonella spp. should not be disregarded as contaminant organisms, particularly when they are isolated in pure culture from clinical specimens involving cases of septic arthritis. | 2001 | 11453595 |
| validation of an in vitro biofilm model of supragingival plaque. | the study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. we describe a model of supragingival plaque containing actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to al ... | 2001 | 11269730 |
| spatial arrangements and associative behavior of species in an in vitro oral biofilm model. | the spatial arrangements and associative behavior of actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis strains in an in vitro model of supragingival plaque were determined. using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. the volume-derived cell numbers of each strain correlated w ... | 2001 | 11229930 |
| competition for glucose between candida albicans and oral bacteria grown in mixed culture in a chemostat. | the competition for glucose as a growth-limiting substrate between candida albicans and a mixed community of oral bacteria was investigated. a chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. a mixed population of oral bacteria was established and after a steady state had been reached the chemostat was inoculated with c. albicans. seven bacterial species streptococcus sanguis, s. sobrinus, s. mitis, lactobacillus casei, veillonella dispar, ... | 2000 | 11073150 |
| potential role of veillonella spp. as a reservoir of transferable tetracycline resistance in the oral cavity. | twelve out of 96 veillonella spp. isolated from oral samples harbored tetracycline resistance genes. the most common resistance gene was tet(m). a tet(m)-positive veillonella dispar strain was shown to transfer a tn916-like element to four streptococcus spp. by conjugation at a frequency of 5.2 x 10(-6) to 4.5 x 10(-5) per recipient. | 2006 | 16870789 |
| the formation of mixed culture biofilms of oral species along a gradient of shear stress. | a chemostat mixed culture system was used to produce two distinct ecological states, state-1 (caries-like microcosm) and state-2 (periodontal-like microcosm). eleven bacterial species (streptococcus gordonii, strep. mitis i, strep. mutans, strep. oralis, actinomyces naeslundii, lactobacillus casei, neisseria subflava, fusobacterium nucleatum, porphyromonas gingivalis, prevotella nigrescens, veillonella dispar) were used to inoculate the planktonic system. a flow cell, designed to produce converg ... | 2000 | 11054158 |
| automated immunofluorescence for enumeration of selected taxa in supragingival dental plaque. | the present study investigated a recently developed automated image analysis technique for its applicability to the enumeration of selected bacteria in supragingival dental plaque. following initial calibration, the system is capable to count fluorescence-labeled target cells in up to 48 samples without user interference. test samples contained a characteristic mixture of planktonic bacteria, small almost planar bacterial aggregates, and large, virtually indisruptable clumps with cells from mult ... | 2000 | 11037755 |
| analysis of ph-driven disruption of oral microbial communities in vitro. | previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ... | 1998 | 9745120 |
| study of root caries in an artificial mouth. | the object of this project was to develop a laboratory model system to study root and enamel caries. model plaque biofilms containing consortia of four (streptococcus mutans, streptococcus sobrinus, actinomyces naeslundii, and lactobacillus rhamnosus) or six (s mutans, s sobrinus, s mitis, a naeslundii, l rhamnosus, and veillonella dispar) species of caries pathogens were developed in a artificial mouth. caries lesions similar to those found in vivo were produced on enamel and dentine blocks and ... | 1998 | 9676475 |
| differentiation of veillonella atypica, veillonella dispar and veillonella parvula using restricted fragment-length polymorphism analysis of 16s rdna amplified by polymerase chain reaction. | veillonella atypica, veillonella dispar and veillonella parvula cannot be reliably distinguished by conventional phenotypic tests, including the api zym test. in this study, restricted fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction (pcr) was used to generate restriction profiles of the type strains of v. atypica, v. dispar and v. parvula and 20 veillonella strains isolated from oral sites. 16s rrna gene sequences from isolated genomic dna samples ... | 1997 | 9573809 |
| prosthetic valve endocarditis due to veillonella dispar: successful medical treatment following penicillin desensitization. | | 1997 | 9142818 |
| the establishment of a community of oral bacteria that controls the growth of candida albicans in a chemostat. | the purpose of this study was to establish and identify a community of oral bacteria that controls the growth of candida albicans in the chemostat. the chemostat was operated under glucose-limiting conditions at a dilution rate of 0.05 h-1 and inoculated with a yeast-free suspension of a tongue scraping. after a steady state had been reached, it was inoculated with c. albicans to establish the yeast and determine whether its growth could be contained. the steady-state community comprised the spe ... | 1996 | 8941776 |
| the effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system. | in order to develop an improved method to evaluate antimicrobial agents for use in clinical dentistry, a constant-depth film fermenter (cdff) has been used to generate biofilms of fixed depth comprising nine species of bacteria commonly found in dental plaque in health and disease. these bacteria were grown together initially in a conventional chemostat which was used to inoculate the cdff over an 8 h period. medium was then supplied directly to the cdff and biofilms allowed to develop. the biof ... | 1996 | 8760321 |
| microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice. | a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ... | 1995 | 8602338 |
| bacteriolysis of veillonella alcalescens by lysozyme and inorganic anions present in saliva. | veillonella alcalescens subsp. dispar was grown in a synthetic medium containing either radiolabeled thymidine or uridine to monitor cell lysis by assay of the release of deoxyribonucleic acid or ribonucleic acid (rna), respectively. biochemical analyses demonstrated that, although human or hen egg white lysozymes alone did not release deoxyribonucleic acid or rna, the nucleic acids were liberated in equal amounts from lysozyme-treated cells by the addition of low concentrations of the sodium sa ... | 1981 | 6166567 |
| coaggregation properties of human oral veillonella spp.: relationship to colonization site and oral ecology. | the primary habitats of oral veillonellae are the tongue, dental plaque, and the buccal mucosa. isolates were obtained from each habitat and tested for coaggregation with a battery of other oral bacterial strains. all 59 tongue isolates tested for coaggregation were veillonella atypica or veillonella dispar. all but one of them coaggregated with strains of streptococcus salivarius, a predominant inhabitant of the tongue surface but not subgingival dental plaque. these tongue isolates were unable ... | 1988 | 3178207 |
| l-serine enhances the anaerobic lactate metabolism of veillonella dispar atcc 17745. | under anaerobic conditions, the rate of metabolism of lactate by starved resting cells of veillonella dispar atcc 17745 was very low. because pyruvate was metabolized well by the starved cells, oxidation of lactate to pyruvate, which is the first step of the lactate metabolism, must have been limited in the cells. in the starved cells, the levels of the metabolic intermediates, oxalacetate or fumarate, of which reductions to malate or to succinate could be coupled with lactate oxidation to pyruv ... | 1987 | 3114345 |
| production and degradation of formate by veillonella dispar atcc 17745. | under strictly anaerobic conditions, the resting cells of v. dispar atcc 17745 produced formate as well as acetate and propionate from pyruvate or from lactate. pyruvate formate-lyase activity was found when the activity was assayed under strictly anaerobic conditions. under aerobic conditions, however, the resting cells did not produce formate from pyruvate or from lactate, though the cells actively metabolized pyruvate or lactate (mainly to acetate). this was ascribed to pyruvate formate-lyase ... | 1986 | 3086409 |
| colonization resistance of defined bacterial plaques to streptococcus mutans implantation on teeth in a model mouth. | we investigated the ability of streptococcus mutans c67-1 to colonize simple bacterial plaques and the effects of age and stability of the pre-formed plaque on colonization resistance. mixed-plaques of actinomyces viscosus wvu627, 'streptococcus mitior' lpa-1, and veillonella dispar omz193 were grown on tooth segments, mounted back to back for simulation of approximal sites in a model mouth for 66 h. s. mutans c67-1 was either included in the original inoculum or super-inoculated onto the develo ... | 1990 | 2307751 |
| endocarditis caused by veillonella dispar. | | 1990 | 2273279 |
| an oral multispecies biofilm model for high content screening applications. | peri-implantitis caused by multispecies biofilms is a major complication in dental implant treatment. the bacterial infection surrounding dental implants can lead to bone loss and, in turn, to implant failure. a promising strategy to prevent these common complications is the development of implant surfaces that inhibit biofilm development. a reproducible and easy-to-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefo ... | 2017 | 28296966 |
| effect of nutrients on defined bacterial plaques and streptococcus mutans c67-1 implantation in a model mouth. | actinomyces viscosus wvu 627, streptococcus oralis lpa-1 and veillonella dispar omz 193 were cocultured on teeth in a model mouth for 66 h. synthetic saliva containing bovine salivary glycoprotein supported bacterial growth, although the delivery of an intermittent nutrient supplement, containing 1% (w/v) glucose or sucrose, gave greater bacterial cell and viable counts. when streptococcus mutans c67-1 was super-inoculated onto 24-hour mixed plaques, it became established under all regimens, but ... | 1991 | 2059972 |
| sugar, fluoride, ph and microbial homeostasis in dental plaque. | factors that may contribute to the maintenance or breakdown of the oral microflora have been studied in the laboratory using a mixed culture chemostat system. carbohydrate type had relatively little influence of the proportions of individual species at neutral ph. in contrast, when the ph was allowed to fall following carbohydrate metabolism, the stability of the microflora was markedly perturbed. the proportions of streptococcus mutans, lactobacillus casei and veillonella dispar increased and t ... | 1991 | 1775479 |
| effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems. | oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (chx) gluconate-containing mouthwash (chxm) diluted to 0.06% (wt/vol) antimicrobial content. the microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge). chxm caused significant decrease ... | 2003 | 12902270 |
| multiplex fish analysis of a six-species bacterial biofilm. | established procedures use different and seemingly incompatible experimental protocols for fluorescent in situ hybridization (fish) with gram-negative and gram-positive bacteria. the aim of this study was to develop a procedure, based on fish and confocal laser scanning microscopy (clsm), for the analysis of the spatial organization of in vitro biofilms containing both gram-negative and gram-positive oral bacteria. biofilms composed of the six oral species actinomyces naeslundii, candida albican ... | 2004 | 14706749 |
| selection for high-level resistance by chronic triclosan exposure is not universal. | to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. | 2004 | 15117935 |
| evaluation of bacterial nitrate reduction in the human oral cavity. | it is hypothesized that the enterosalivary nitrate circulation encourages nitrate reducing bacteria to reside within the oral cavity. nitrite production may then limit the growth of acidogenic bacteria as a result of the production of antimicrobial oxides of nitrogen, including nitric oxide. this study was carried out with 10 subjects to characterize oral nitrate reduction and identify the bacteria responsible. nitrate reduction varied between individuals (mean 85.4 +/- 15.9 nmol nitrite min(-1) ... | 2005 | 15693824 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| effects of streptococcus mutans gtfc deficiency on mixed oral biofilms in vitro. | the aim of this study was to examine the influence of glucosyltransferase-gene-negative (gtf-) streptococcus mutans strains unable to synthesize water-insoluble or soluble glucan on the structure and macromolecular diffusion properties of in vitro grown mixed oral biofilms. biofilms modeling supragingival plaque consisted of actinomyces naeslundii omz 745, candida albicans omz 110, fusobacterium nucleatum kp-f2, streptococcus oralis sk 248, veillonella dispar atcc 17748t and one of the s. mutans ... | 2006 | 16508276 |
| the genus veillonella iv. serological groupings, and genus and species emendations. | rogosa, m. (national institute of dental research, bethesda, md.). the genus veillonella. iv. serological groupings, and genus and species emendations. j. bacteriol. 90:704-709. 1965.-seven serological groups of veillonella were found. the generic and species descriptions are enlarged and corrected. the type species is veillonella parvula (veillon and zuber) prévot, 1933. the proposed species, subspecies (infraspecific serogroups), and american type culture collection (atcc) type strains are: ve ... | 1965 | 16562069 |
| a novel tem contrasting technique for extracellular polysaccharides in in vitro biofilms. | in the past, the visualization of the extracellular matrix of biofilms on an ultrastructural level was hampered by shrinkage artifacts. in addition, the reproducible contrasting of extracellular polysaccharides (eps) has not satisfactorily been solved. here we describe a method overcoming these difficulties, which produces artifact-free transmission electron microscopic (tem) images using multispecies biofilms grown in vitro. sufficient contrast was achieved by replacing schiff's reagent with th ... | 2007 | 17557287 |
| effect of chlorhexidine/thymol and fluoride varnishes on dental biofilm formation in vitro. | this study evaluated the effect of chlorhexidine/thymol (chx/t) and fluoride (f) varnishes on biofilm formation in vitro. hydroxyapatite discs coated with varnish were first immersed in saline for 0, 3, 7 or 14 d, then immersed in pasteurized saliva. the discs were incubated for 20 h with a bacterial suspension containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, and veillonella dispar. uncoated discs were used as controls. growth of bacteria on the discs was evaluat ... | 2007 | 18028054 |
| molecular identification of bacteria on the tongue dorsum of subjects with and without halitosis. | compare the microbial profiles on the tongue dorsum in patients with halitosis and control subjects in a uk population using culture-independent techniques. | 2008 | 18336372 |
| the predominant cultivable veillonella spp. of the tongue of healthy adults identified using rpob sequencing. | the predominant veillonella spp. were isolated from the dorsum surface of the tongues of 11 healthy adults and identified to species level using rpob sequencing because 16s ribosomal rna sequence analysis does not reliably differentiate between all members of this genus. in all, 253 isolates were identified and the mean proportion (+/- se) of veillonella spp. per sample was 16.2 (+/- 3.6) with a range of 3.0% to 36.3% of the total anaerobic colony count. the predominant species were veillonella ... | 2008 | 18582335 |
| identification of oral species of the genus veillonella by polymerase chain reaction. | members of the genus veillonella cannot be reliably distinguished by their biochemical characteristics and phenotypic features. moreover, dna-dna hybridization and sequence analyses of the 16s ribosomal rna gene including random fragment length polymorphism analysis, are complex and time-consuming procedures that are not well-suited to identifying oral species of veillonella: veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, and veillonella rogosae. | 2009 | 19572893 |
| transfer of antibiotic resistance by transformation with edna within oral biofilms. | we demonstrate that live donor veillonella dispar cells can transfer the conjugative transposon tn916 to four different streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. additionally, we demonstrate that purified v. dispar dna can transform streptococcus mitis to tetracycline resistance in this experimental system. these data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformatio ... | 2010 | 20337719 |
| validation of a quantitative real-time pcr assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model. | subgingival biofilms are the prime etiological factor of periodontal disease. owing to their complex polymicrobial nature, quantification of individual bacterial species within the biofilm for research and diagnostic purposes can be methodologically challenging. the aims of this study were to establish a quantitative real-time pcr (qpcr) assay to quantify the bacteria used in our 10-species in vitro 'subgingival' biofilm model and to compare the quantitative outcome with fluorescence microscopy ... | 2012 | 23278531 |
| impact of early colonizers on in vitro subgingival biofilm formation. | the aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. the model included streptococcus oralis, streptococcus anginosus, actinomycesoris, fusobacterium nucleatum subsp. nucleatum, veillonella dispar, campylobacter rectus, prevotella intermedia, porphyromonas gingivalis, tannerella forsythia, and treponema denticola. based on literature, we consider ... | 2013 | 24340084 |
| effects of ph and lactate on hydrogen sulfide production by oral veillonella spp. | indigenous oral bacteria in the tongue coating such as veillonella have been identified as the main producers of hydrogen sulfide (h2s), one of the major components of oral malodor. however, there is little information on the physiological properties of h2s production by oral veillonella such as metabolic activity and oral environmental factors which may affect h2s production. thus, in the present study, the h2s-producing activity of growing cells, resting cells, and cell extracts of oral veillo ... | 2014 | 24795374 |
| incorporation of staphylococci into titanium-grown biofilms: an in vitro "submucosal" biofilm model for peri-implantitis. | staphylococcus spp. are postulated to play a role in peri-implantitis. this study aimed to develop a "submucosal" in vitro biofilm model, by integrating two staphylococci into its composition. | 2015 | 26461083 |
| establishment of an oral infection model resembling the periodontal pocket in a perfusion bioreactor system. | periodontal infection involves a complex interplay between oral biofilms, gingival tissues and cells of the immune system in a dynamic microenvironment. a humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in a periodontal pocket, would constitute a technical advancement in the study of periodontal disease. the aim of this study was to use a dynamic perfusion bioreactor in order to develop a gingival epithelial-fibroblast-mon ... | 2015 | 25587671 |
| pulp and plaque microbiotas of children with severe early childhood caries. | bacterial invasion into pulps of primary teeth can lead to infection and premature tooth loss in children. this pilot study aimed to explore whether the microbiota of carious exposures of dental pulps resembles that of carious dentin or that of infected root canals. | 2015 | 25651832 |
| in vitro culture of previously uncultured oral bacterial phylotypes. | around a third of oral bacteria cannot be grown using conventional bacteriological culture media. community profiling targeting 16s rrna and shotgun metagenomics methods have proved valuable in revealing the complexity of the oral bacterial community. studies investigating the role of oral bacteria in health and disease require phenotypic characterizations that are possible only with live cultures. the aim of this study was to develop novel culture media and use an in vitro biofilm model to cult ... | 2015 | 26407883 |
| in vitro effect of porphyromonas gingivalis methionine gamma lyase on biofilm composition and oral inflammatory response. | methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. the aim of this study was to compare an mgl knockout strain of p. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and infla ... | 2016 | 28033374 |
| microbiological diversity of peri-implantitis biofilm by sanger sequencing. | to examine the microbial diversity associated with implants with or without peri-implantitis and to identify differences between the bacterial communities in these clinical conditions. | 2014 | 23845046 |
| salivary pellicle composition and multispecies biofilm developed on titanium nitrided by cold plasma. | the aim of this study was to evaluate the composition of the salivary pellicle (sp) and multispecies biofilm developed on titanium nitrided by cold plasma. | 2014 | 24769315 |
| colonisation of gingival epithelia by subgingival biofilms in vitro: role of "red complex" bacteria. | biofilm formation on tooth surface results in colonisation and invasion of the juxtaposed gingival tissue, eliciting strong inflammatory responses that lead to periodontal disease. this in vitro study investigated the colonisation of human gingival multi-layered epithelium by multi-species subgingival biofilms, and evaluated the relative effects of the "red complex" species (porphyromonas gingivalis, tannerella forsythia and treponema denticola). | 2014 | 24949828 |
| red wine and oenological extracts display antimicrobial effects in an oral bacteria biofilm model. | the antimicrobial effects of red wine and its inherent components on oral microbiota were studied by using a 5-species biofilm model of the supragingival plaque that includes actinomyces oris, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar. microbiological analysis (cfu counting and confocal laser scanning microscopy) of the biofilms after the application of red wine, dealcoholized red wine, and red wine extract solutions spiked or not with grape seed ... | 2014 | 24773294 |
| next-generation sequencing combined with specific pcr assays to determine the bacterial 16s rrna gene profiles of middle ear fluid collected from children with acute otitis media. | the aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged 5 to 42 months (median age, 19 months). the bacteriome profiles of middle ear fluid samples were determined by a nested-pcr amplification of the 16s rrna gene (v4 region), followed by mass sequencing. the prof ... | 2017 | 28357413 |
| taxa of the nasal microbiome are associated with influenza-specific iga response to live attenuated influenza vaccine. | live attenuated influenza vaccine (laiv) has demonstrated varying levels of efficacy against seasonal influenza; however, laiv may be used as a tool to measure interactions between the human microbiome and a live, replicating virus. to increase our knowledge of this interaction, we measured changes to the nasal microbiome in subjects who received laiv to determine if associations between influenza-specific iga production and the nasal microbiome exist after immunization with a live virus vaccine ... | 2016 | 27643883 |
| subgingival microbiome in smokers and non-smokers in korean chronic periodontitis patients. | smoking is a major environmental factor associated with periodontal diseases. however, we still have a very limited understanding of the relationship between smoking and subgingival microflora in the global population. here, we investigated the composition of subgingival bacterial communities from the pooled plaque samples of smokers and non-smokers, 134 samples in each group, in korean patients with moderate chronic periodontitis using 16s rrna gene-based pyrosequencing. a total of 17,927 reads ... | 2015 | 25283067 |
| community-level differences in the microbiome of healthy wild mallards and those infected by influenza a viruses. | waterfowl, especially ducks and geese, are primary reservoirs for influenza a viruses (iavs) that evolve and emerge as important pathogens in domestic animals and humans. in contrast to humans, where iavs infect the respiratory tract and cause significant morbidity and mortality, iavs infect the gastrointestinal tract of waterfowl and cause little or no pathology and are spread by fecal-oral transmission. for this reason, we examined whether iav infection is associated with differences in the cl ... | 2017 | 28293681 |
| comparison of human gut microbiota in control subjects and patients with colorectal carcinoma in adenoma: terminal restriction fragment length polymorphism and next-generation sequencing analyses. | colorectal cancer (crc) is the third leading cause of cancer-related deaths in japan. the etiology of crc has been linked to numerous factors including genetic mutation, diet, life style, inflammation, and recently, the gut microbiota. however, crc-associated gut microbiota is still largely unexamined. this study used terminal restriction fragment length polymorphism (t-rflp) and next-generation sequencing (ngs) to analyze and compare gut microbiota of japanese control subjects and japanese pati ... | 2016 | 26549775 |
| microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model. | the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ... | 2016 | 26033167 |
| in vitro growth characteristics and volatile sulfur compound production of solobacterium moorei. | solobacterium moorei has recently been implicated as a causative agent of halitosis. in vitro experiments to evaluate the role of s. moorei in halitosis have, however, been complicated by a paucity of information on the ideal conditions for culturing this organism. this work aimed to optimize a liquid culture medium for s. moorei, and to determine the growth-curve of the organism. further, the ability of s. moorei to generate volatile sulfur compounds was investigated and compared quantitatively ... | 2014 | 24487184 |
| the influence of oral veillonella species on biofilms formed by streptococcus species. | oral veillonella, veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, veillonella rogosae, and veillonella tobetsuensis are known as early colonizers in oral biofilm formation. to investigate the role of oral veillonella, biofilms formed by the co-culture of streptococcus gordonii, streptococcus mutans, streptococcus salivarius, or streptococcus sanguinis, with oral veillonella were examined at the species level. the amount of biofilm formed by s. mutans, s. g ... | 2014 | 24862495 |
| identification of veillonella tobetsuensis in tongue biofilm by using a species-specific primer pair. | veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, and veillonella rogosae have been reported to be isolated from human oral cavities. the recently detected veillonellatobetsuensis in human tongue biofilms was proposed as a novel veillonella sp. in this study, to determine the distribution and frequency of v. tobetsuensis, we established a method for the detection and identification of v. tobetsuensis by using polymerase chain reaction (pcr) using a species-s ... | 2013 | 23664905 |