| determination of the 5' and 3' terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus. | terminal sequences of the bi-segmented dsrna genome of 3 different strains of infectious bursal disease virus (ibdv) were analyzed by the rapid amplification of cdna 5' ends (5'race) procedure. both segments are 85% homologous in a 32-nucleotide sequence comprising the 5' end, whereas the 3' end has a conserved pentamer. comparison to published terminal sequences of other ibdv strains revealed high conservation between the two segments but more serotype-specific nucleotide changes (5 on segment ... | 1996 | 8712930 |
| sequence analysis of the bicistronic drosophila x virus genome segment a and its encoded polypeptides. | drosophila x virus represents the entomobirnavirus genus of the family birnaviridae. segment a of this bisegmented dsrna containing virus was cloned and sequenced. the 3360-bp-long nucleotide sequence revealed the presence of two open reading frames (orfs). a large orf of 3096 nucleotides, which is flanked by a 107-bp 5' and a 157-bp 3'-untranslated region, and a 711-nucleotide-long small orf located within the carboxy half of the large orf but in a different reading frame. the large orf encodes ... | 1996 | 8918922 |
| site-directed mutagenesis of avibirnavirus vp4 gene. | virus protein vp4 of infectious bursal disease virus (ibdv) is a protease which separates vpx and vp3 from the polyprotein. we studied the importance of serine and aspartic acid on cleavage at the vpx/vp4 junction and analysed the role of the proposed h547, d590, and s653 catalytic site using five different mutations on vp4. our results suggest that the replacement of serine by lysine in axaas motifs in serotype ii ibdv influences polyprotein (pp) processing by vp4 and also indicate the presence ... | 2002 | 11878927 |
| birnavirus vp1 proteins form a distinct subgroup of rna-dependent rna polymerases lacking a gdd motif. | we have cloned and characterized the drosophila x virus (dxv) genome segment b and its encoded vp1, the putative rna-dependent rna polymerase (rdrp) present in the virion. the 2991-bp open reading frame encodes the largest birnavirus vp1 at 977 aa, with a calculated m(r) of 112.8 kda. as with the vp1 proteins of the type species of the other two genera in the family birnaviridae, namely, infectious pancreatic necrosis virus (genus aquabirnavirus) and infectious bursal disease virus (genus avibir ... | 2002 | 12069523 |
| blotched snakehead virus is a new aquatic birnavirus that is slightly more related to avibirnavirus than to aquabirnavirus. | by different approaches, we characterized the birnavirus blotched snakehead virus (bsnv). the sequence of genomic segment a revealed the presence of two open reading frames (orfs): a large orf with a 3,207-bp-long nucleotide sequence and a 417-nucleotide-long small orf located within the n-terminal half of the large orf, but in a different reading frame. the large orf was found to encode a polyprotein cotranslationally processed by the viral protease vp4 to generate pvp2 (the vp2 precursor), a 7 ... | 2003 | 12477876 |
| comparison of the rna polymerase genes of marine birnavirus strains and other birnaviruses. | the cdna nucleotide sequence of the genome segment b encoding the vp1 protein, the putative rna-dependent rna polymerase (rdrp), was determined for 5 marine birnavirus (mabv) strains from different host or geographic origins and 1 infectious pancreatic necrosis virus (ipnv) strain am-98. segment b of the ipnv am-98 strain and 4 mabv strains, y-6, yt-01a, h1 and nc1, contained a 2535 bp orf, which encoded a protein of 845 amino acid residues with a predicted mw of 94.4 kda. only the mabv ay-98 rd ... | 2003 | 12664297 |
| reassortant infectious bursal disease virus isolated in china. | infectious bursal disease virus (ibdv) is a bi-segmented, double-stranded rna virus which belongs to the genus avibirnavirus of the family birnavirideae. in this study, we determined the complete nucleotide sequences of a reassortment ibdv strain tl2004 with segments a and b derived from attenuated and very virulent strains of ibdv. this strain is pathogenic to spf-embryonated eggs and chickens, although it is not as virulent as very virulent strain. genomic sequence in genbank analysis showed t ... | 2008 | 17945373 |
| effective protection by high efficiency bicistronic dna vaccine against infectious bursal disease virus expressing vp2 protein and chicken il-2. | infectious bursal disease (ibd) is an acute and contagious viral infection of young chickens caused by ibd virus (ibdv). ibdv belongs to genus avibirnavirus of family birnaviridae. it is a non-enveloped virus with icosahedral symmetry that contains two segments of double-stranded rna. the virus affects the lymphoid tissues of chickens, mainly the b cells of bursa of fabricius, leading to severe and prolonged immunosuppression. vp2, a major structural protein of ibdv, contains antigenic epitopes ... | 2009 | 19111591 |
| [cloning of infectious bursal disease virus (gt strain) lack of vp5 gene]. | infectious bursal disease virus (ibdv) belongs to genus avibirnavirus of the family birnaviridae. the genome of ibdv consists of two segments of double-strand rna, which encode four structural protein vp1-vp4 and one non-structural protein vp5. | 2008 | 19271543 |
| exchange of the vp5 of infectious bursal disease virus in a serotype i strain with that of a serotype ii strain reduced the viral replication and cytotoxicity. | infectious bursal disease virus (ibdv), belonging to avibirnavirus genus in the birnaviridae family, consists of two segments of double-strand rna. there are two distinct serotypes of ibdv, the pathogenic serotype i and the non-pathogenic serotype ii. comparison of the deduced amino acid sequences of a panel of vp5 genes retrieved from genbank revealed a high identity among strains within the serotype i or serotype ii group but a low identity between strains across two serotypes. in this study, ... | 2009 | 19557352 |
| optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of fabricius. | two-dimensional gel electrophoresis (2-de) is a powerful method to study protein expression and function in living organisms and diseases. this technique, however, has not been applied to avian bursa of fabricius (bf), a central immune organ. here, optimized 2-de sample preparation methodologies were constructed for the chicken bf tissue. using the optimized protocol, we performed further 2-de analysis on a soluble protein extract from the bf of chickens infected with virulent avibirnavirus. to ... | 2009 | 19814785 |
| development and validation of four real-time quantitative rt-pcrs specific for the positive or negative strands of a bisegmented dsrna viral genome. | four tagged quantitative real-time rt-pcrs (qrt-pcrs) were developed to quantify the positive and negative strands of segments a and b of the bisegmented double-stranded rna (dsrna) genome of infectious bursal disease virus (ibdv, family birnaviridae, genus avibirnavirus). the qrt-pcrs were validated using single-stranded rnas corresponding to each genomic strand (a+, b+, a-, b-). specific quantitation proved possible from 5×10(7) to 5×10(2) copies of the template per reaction, with excellent re ... | 2010 | 20638414 |