| rpob-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. | nonpigmented and late-pigmenting rapidly growing mycobacteria (rgm) are increasingly isolated in clinical microbiology laboratories. their accurate identification remains problematic because classification is labor intensive work and because new taxa are not often incorporated into classification databases. also, 16s rrna gene sequence analysis underestimates rgm diversity and does not distinguish between all taxa. we determined the complete nucleotide sequence of the rpob gene, which encodes th ... | 2003 | 14662964 |
| [postoperative sternal osteomyelitis due to mycobacterium porcinum]. | | 2007 | 17261250 |
| [microbiological properties of m. porcinum isolated from a patient with impairments in il-12/ifn-gamma pathway]. | mycobacterium porcinum has been successfully isolated from the patient with abnormal signal transduction pathway of il12/ifn-gamma. the properties of each bacterium were determined by conventional identification methods, dna sequencing analysis and mic assay. | 2008 | 19086435 |
| detection of lfra and tap efflux pump genes among clinical isolates of non-pigmented rapidly growing mycobacteria. | this study was performed to detect lfra and tap efflux pumps among clinical isolates of non-pigmented rapidly growing mycobacteria (nprgm). gene detection was performed using polymerase chain reaction (pcr) with specific primers designed for each gene. susceptibility of the strains to doxycycline, tigecycline and ciprofloxacin was analysed using the broth microdilution reference technique. in total, 166 clinical isolates were included in the study. the lfra gene was detected in four strains (2.4 ... | 2009 | 19665358 |
| nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of rondônia, brazil. | the main cause of pulmonary tuberculosis (tb) is infection with mycobacterium tuberculosis (mtb). we aimed to evaluate the contribution of nontuberculous mycobacteria (ntm) to pulmonary disease in patients from the state of rondônia using respiratory samples and epidemiological data from tb cases. mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. among the 1,812 ca ... | 2013 | 23827995 |
| application of four molecular typing methods for analysis of mycobacterium fortuitum group strains causing post-mammaplasty infections. | a cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in campinas, in the southern region of brazil. rapidly growing mycobacteria were isolated from samples from 12 patients. eleven isolates were identified as mycobacterium fortuitum and one as mycobacterium porcinum by pcr-restriction digestion of the hsp65 gene. these 12 isolates, plus six additional m. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophor ... | 2006 | 16441452 |
| clinical and laboratory features of mycobacterium porcinum. | recent molecular studies have shown mycobacterium porcinum, recovered from cases of lymphadenitis in swine, to have complete 16s rdna sequence identity and >70% dna-dna homology with human isolates within the m. fortuitum third biovariant complex. we identified 67 clinical and two environmental isolates of the m. fortuitum third biovariant sorbitol-negative group, of which 48 (70%) had the same pcr restriction enzyme analysis (pra) profile as the hsp65 gene of m. porcinum (atcc 33776(t)) and wer ... | 2004 | 15583300 |
| taxonomic variation in the mycobacterium fortuitum third biovariant complex: description of mycobacterium boenickei sp. nov., mycobacterium houstonense sp. nov., mycobacterium neworleansense sp. nov. and mycobacterium brisbanense sp. nov. and recognition of mycobacterium porcinum from human clinical isolates. | the mycobacterium fortuitum third biovariant complex (sorbitol-negative and sorbitol-positive) contains unnamed taxa first characterized in 1991. these organisms can cause respiratory infections, a spectrum of soft tissue and skeletal infections, bacteraemia and disseminated disease. to evaluate this group of organisms, clinical reference isolates and the type strains of m. fortuitum third biovariant complex sorbitol-negative (n = 21), m. fortuitum third biovariant complex sorbitol-positive (n = ... | 2004 | 15388725 |
| structures of the glycopeptidolipid antigens of two animal pathogens: mycobacterium senegalense and mycobacterium porcinum. | the structures of the major glycolipid antigens of two animal pathogens mycobacterium senegalense and mycobacterium porcinum were elucidated by a combination of fast-atom bombardment mass spectrometry, nuclear magnetic resonance spectroscopy, chemical analyses and radiolabeling experiments. five glycoconjugates belonging to the class of c-mycoside glycopeptidolipids were characterized in each species. they shared with those recently described in m. peregrinum the same unusual distribution of the ... | 1993 | 8354291 |
| distribution of a novel mycolic acid in species of the genus mycobacterium. | we found that mycobacterium porcinum atcc 33776t (t = type strain) contains a new kind of mycolic acid with a methoxy group at the omega-1 position. this mycolic acid was identified by comparing it with the previously described methoxymycolic acids. the patterns of mycolic acid methyl esters from 418 strains belonging to 44 species of mycobacteria were studied by using thin-layer chromatography. in addition to m. procinum atcc 33776t, representative strains of m. porcinum, mycobacterium fortuitu ... | 1991 | 1883713 |
| species-specific risk factors, treatment decisions, and clinical outcomes for laboratory isolates of less common nontuberculous mycobacteria in washington state. | nontuberculous mycobacteria (ntm) are a diverse group of environmental organisms that infrequently cause human disease. understanding of the epidemiologic and clinical characteristics associated with ntm disease is needed to refine diagnostic and treatment strategies, particularly among the less commonly isolated species. | 2017 | 28387523 |
| verification of frequency in species of nontuberculous mycobacteria in kermanshah drinking water supplies using the pcr-sequencing method. | nontuberculous mycobacteria are habitants of environment, especially in aquatic systems. some of them cause problems in immunodeficient patients. over the last decade, 16s rrna gene sequencing was established in 45 novel species of nontuberculous mycobacteria. experiences revealed that this method underestimates the diversity, but does not distinguish between some of mycobacterium subsp. to recognize emerging rapidly growing mycobacteria and identify their subsp, rpob gene sequencing has been de ... | 2017 | 27668545 |
| rapidly growing mycobacteria in tb/hiv co-infection: a report of two cases focusing on difficulties in diagnosis and management. | recent reports indicate an increase in rates of infection and disease due to rapidly growing mycobacteria (rgm) in patients with pre-existing chronic lung disease. studies have described difficulties in correctly identifying closely related species, even when proper methodologies are adopted, and several different gene targets have been proposed. we describe two cases of rgm infection in a 29-year-old hiv-1 positive congolese man and a 19-year-old hiv-1 positive liberian woman, respectively, bot ... | 2012 | 22707139 |
| epidemiology of nontuberculous mycobacteria in french polynesia. | as few data are available in the pacific countries and territories of the oceania region regarding nontuberculous mycobacteria,we retrospectively identified 87 such isolates from french polynesia from 2008 to 2013 by hybridization using dna-strip, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and partial rpob gene sequencing. partial rpob gene sequencing classified 42/87 (48.3%) isolates in the mycobacterium fortuitum complex, 28 (32.2%) in the mycob ... | 2015 | 26400787 |
| five-year outbreak of community- and hospital-acquired mycobacterium porcinum infections related to public water supplies. | mycobacterium porcinum is a rarely encountered rapidly growing mycobacterium (rgm). we identified m. porcinum from 24 patients at a galveston university hospital (university of texas medical branch) over a 5-year period. m. porcinum was considered a pathogen in 11 (46%) of 24 infected patients, including 4 patients with community-acquired disease. retrospective patient data were collected, and water samples were cultured. molecular analysis of water isolates, clustered clinical isolates, and 15 ... | 2011 | 21998415 |
| mycobacterium porcinum peritonitis in a patient on continuous ambulatory peritoneal dialysis. | mycobacterium porcinum has been reported to cause a variety of illnesses including wound infections, respiratory tract infections, osteomyelitis and catheter-related bacteremias. we report the first case of m. porcinum peritonitis in a patient on continuous ambulatory peritoneal dialysis (capd). a 67-year-old woman on capd presented with three weeks of constitutional symptoms and abdominal pain. peritoneal fluid cultures on day three grew acid-fast rods. nocardiosis was suspected and the patient ... | 2010 | 21104454 |
| mycobacterium porcinum strains isolated from bovine bulk milk: implications for mycobacterium avium subsp. paratuberculosis detection by pcr and culture. | in this study, the isolation of 52 mycobactin-independent fast growing mycobacteria from 631 bulk milk samples (8.2%), is reported. these strains, isolated during a bulk milk survey for mycobacterium avium subsp. paratuberculosis (map), strongly affected map detection both by pcr and by culture, as they gave a positive is900 pcr signal and resulted to totally inhibit the growth of map when spotted on heym slants already inoculated with 200 microl of 10-fold dilutions containing from 5 x 10 to 5 ... | 2008 | 18378411 |
| description of mycobacterium conceptionense sp. nov., a mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation. | a nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. the three isolates grew in 3 days at 24 to 37 degrees c. 16s rrna sequence analyses over 1,483 bp showed that they were identical and shared 99.7% (4-bp difference) sequence similarity with that of mycobacterium porcinum, the most closely related species. partia ... | 2006 | 16597850 |