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detection of mycobacteria in joint samples from patients with arthritis using a genus-specific polymerase chain reaction and sequence analysis.mycobacteria have been implicated in the pathogenesis of various forms of arthritis. the aim of this study was to examine the diagnostic potential of molecular biological techniques as well as to investigate the pathogenetic role of mycobacteria in chronic arthritis.199910402076
biodegradation of methyl tert-butyl ether and other fuel oxygenates by a new strain, mycobacterium austroafricanum ifp 2012.a strain that efficiently degraded methyl tert-butyl ether (mtbe) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (tba). this strain, a gram-positive methylotrophic bacterium identified as mycobacterium austroafricanum ifp 2012, was also able to degrade tert-amyl methyl ether and tert-amyl alcohol. ethyl tert-butyl ether was weakly degraded. tert-butyl formate and 2-hydroxy isobutyrate (hiba), two intermediates in the mtbe catabolism pathway, were detected durin ...200212039730
degradation of straight-chain aliphatic and high-molecular-weight polycyclic aromatic hydrocarbons by a strain of mycobacterium austroafricanum.our goal was to characterize a newly isolated strain of mycobacterium austroafricanum, obtained from manufactured gas plant (mgp) site soil and designated gti-23, with respect to its ability to degrade polycyclic aromatic hydrocarbons (pahs).200312534814
degradation of isooctane by mycobacterium austroafricanum ifp 2173: growth and catabolic pathway.isooctane (2,2,4-trimethylpentane), a major component of gasoline formulations, is recalcitrant to biodegradation probably because of the quaternary carbon group it contains. information on the biodegradability of this hydrocarbon is essential to evaluate its fate in the environment. for these reasons, the degradation kinetics and the catabolic pathway of isooctane were investigated in mycobacterium austroafricanum ifp 2173, the only strain characterized to use it as sole carbon and energy sourc ...200415281945
isolation and characterization of a new mycobacterium austroafricanum strain, ifp 2015, growing on mtbe.a new mycobacterium austroafricanum strain, ifp 2015, growing on methyl tert-butyl ether (mtbe) as a sole carbon source was isolated from an mtbe-degrading microcosm inoculated with drain water of an mtbe-supplemented gasoline storage tank. m. austroafricanum ifp 2015 was able to grow on tert-butyl formate, tert-butyl alcohol (tba) and alpha-hydroxyisobutyrate. 2-methyl-1,2-propanediol was identified as the tba oxidation product in m. austroafricanum ifp 2015 and in the previously isolated m. au ...200616028043
n-alkane assimilation and tert-butyl alcohol (tba) oxidation capacity in mycobacterium austroafricanum strains.mycobacterium austroafricanum ifp 2012, which grows on methyl tert-butyl ether (mtbe) and on tert-butyl alcohol (tba), the main intermediate of mtbe degradation, also grows on a broad range of n-alkanes (c2 to c16). a single alkb gene copy, encoding a non-heme alkane monooxygenase, was partially amplified from the genome of this bacterium. its expression was induced after growth on n-propane, n-hexane, n-hexadecane and on tba but not after growth on lb. the capacity of other fast-growing mycobac ...200717347817
biodegradability of 2-ethylhexyl nitrate (2-ehn), a cetane improver of diesel oil.the 2-ethyhexyl nitrate (2-ehn) is currently added to diesel oil to improve ignition and boost cetane number. the biodegradability of this widely used chemical needed to be assessed in order to evaluate the environmental impact in case of accidental release. in aerobic liquid cultures, biodegradation of 2-ehn was assessed in biphasic liquid cultures using an inert non-aqueous phase liquid such as 2,2,4,4,6,8,8-heptamethylnonane (hmn) as solvent for the hydrophobic substrate. 2-ehn was found to b ...200918568432
proteomic investigation of enzymes involved in 2-ethylhexyl nitrate biodegradation in mycobacterium austroafricanum ifp 2173.2-ethyhexyl nitrate (2-ehn) is a synthetic chemical used as a diesel fuel additive, which is recalcitrant to biodegradation. in this study, the enzymes involved in 2-ehn degradation were investigated in mycobacterium austroafricanum ifp 2173. using two-dimensional gel electrophoresis and a shotgun proteomic approach, a total of 398 proteins appeared to be more abundant in cells exposed to 2-ehn than in acetate-grown cells. this set of proteins includes multiple isoenzymes of the beta-oxidation p ...200919840846
draft genome sequence of mycobacterium austroafricanum dsm 44191.we announce the draft genome sequence of mycobacterium austroafricanum dsm 44191(t) (= e9789-sa12441(t)), a non-tuberculosis species responsible for opportunistic infection. the genome described here has a size of 6,772,357 bp with a g+c content of 66.79% and contains 6,419 protein-coding genes and 112 rna genes.201424744336
effects of gasoline components on mtbe and tba cometabolism by mycobacterium austroafricanum job5.in this study we have examined the effects of individual gasoline hydrocarbons (c(5-10,12,14) n-alkanes, c(5-8) isoalkanes, alicyclics [cyclopentane and methylcyclopentane] and btex compounds [benzene, toluene, ethylbenzene, m-, o-, and p-xylene]) on cometabolism of methyl tertiary butyl ether (mtbe) and tertiary butyl alcohol (tba) by mycobacterium austroafricanum job5. all of the alkanes tested supported growth and both mtbe and tba oxidation. growth on c(5-8) n-alkanes and isoalkanes was inhi ...201020012341
involvement of a novel enzyme, mdpa, in methyl tert-butyl ether degradation in methylibium petroleiphilum pm1.methylibium petroleiphilum pm1 is a well-characterized environmental strain capable of complete metabolism of the fuel oxygenate methyl tert-butyl ether (mtbe). using a molecular genetic system which we established to study mtbe metabolism by pm1, we demonstrated that the enzyme mdpa is involved in mtbe removal, based on insertional inactivation and complementation studies. mdpa is constitutively expressed at low levels but is strongly induced by mtbe. mdpa is also involved in the regulation of ...200818791002
biodegradation of 2-ethylhexyl nitrate by mycobacterium austroafricanum ifp 2173.2-ethyhexyl nitrate (2-ehn) is a major additive of fuel that is used to increase the cetane number of diesel. because of its wide use and possible accidental release, 2-ehn is a potential pollutant of the environment. in this study, mycobacterium austroafricanum ifp 2173 was selected from among several strains as the best 2-ehn degrader. the 2-ehn biodegradation rate was increased in biphasic cultures where the hydrocarbon was dissolved in an inert non-aqueous-phase liquid, suggesting that the t ...200818723659
use of mycobacterium austroafricanum ifp 2012 in a mtbe-degrading bioreactor.mycobacterium austroafricanum ifp 2012 is able to slowly grow on methyl tert-butyl ether (mtbe), a fuel oxygenate widely used as a gasoline additive. the potential of m. austroafricanum ifp 2012 for aerobic mtbe degradation was investigated in the presence of a secondary carbon source, isopropanol. the strain was then tested for mtbe biodegradation at the laboratory-scale in a fixed-bed reactor using perlite as the matrix, and isopropanol was injected once a week to maintain m. austroafricanum i ...200818685271
pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, graphium sp.the filamentous fungus graphium sp. (atcc 58400) co-metabolically oxidizes the gasoline oxygenate methyl tertiary butyl ether (mtbe) after growth on gaseous n-alkanes. in this study, the enzymology and regulation of mtbe oxidation by propane-grown mycelia of graphium sp. were further investigated and defined. the trends observed during mtbe oxidation closely resembled those described for propane-grown cells of the bacterium mycobacterium vaccae job5. propane-grown mycelia initially oxidized the ...200818043916
isolation and identification of mycobacteria from soils at an illegal dumping site and landfills in japan.in order to study the diversity and community of genus mycobacterium in polluted soils, we tried to isolate mycobacteria from 11 soil samples collected from an illegal dumping site and 3 landfills in japan. using culture methods with or without acanthamoeba culbertsoni, a total of 19 isolates of mycobacteria were obtained from 5 soil samples and 3 of them were isolated only by the co-culture method with the amoeba. conventional biochemical tests and sequencing of the hsp65, rpob, and 16s rrna ge ...200616858142
genes involved in the methyl tert-butyl ether (mtbe) metabolic pathway of mycobacterium austroafricanum ifp 2012.methyl tert-butyl ether (mtbe) is a persistent pollutant of surface and groundwater, and the reasons for its low biodegradability are poorly documented. using one of the rare bacterial strains able to grow in the presence of mtbe, mycobacterium austroafricanum ifp 2012, the protein profiles of crude extracts after growth in the presence of mtbe and glucose were compared by sds-page. ten proteins with molecular masses of 67, 64, 63, 55, 50, 27, 24, 17, 14 and 11 kda were induced after growth in t ...200616622053
occurrence and community composition of fast-growing mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons.fast-growing mycobacteria are considered essential members of the polycyclic aromatic hydrocarbons (pah) degrading bacterial community in pah-contaminated soils. to study the natural role and diversity of the mycobacterium community in contaminated soils, a culture-independent fingerprinting method based on pcr combined with denaturing gradient gel electrophoresis (dgge) was developed. new pcr primers were selected which specifically targeted the 16s rrna genes of fast-growing mycobacteria, and ...200516329885
stimulation of pyrene mineralization in freshwater sediments by bacterial and plant bioaugmentation.as a means to study the fate of polycyclic aromatic hydrocarbons (pahs) in freshwater sediments, pyrene mineralization was examined in microcosms spiked with [14c]pyrene. some microcosms were planted with reeds (phragmites australis) and/or inoculated with a pyrene-degrading strain, mycobacterium sp. 6py1. mineralization rates recorded over a 61 d period showed that reeds promoted a significant enhancement of pyrene degradation, which possibly resulted from a root-mediated increase of oxygen dif ...200516124309
mycobacterium pyrenivorans sp. nov., a novel polycyclic-aromatic-hydrocarbon-degrading species.the taxonomic position of a polycyclic-aromatic-hydrocarbon-degrading bacterium, strain 17a3(t), isolated from contaminated soil was determined using a combination of phenotypic and genotypic properties. the isolate showed phenotypic properties that were diagnostic for species of the genus mycobacterium. comparative 16s rrna gene sequence analysis assigned 17a3(t) to the 16s rrna gene subgroup that contains mycobacterium aurum, mycobacterium austroafricanum, mycobacterium vaccae and mycobacteriu ...200415545477
roles of tert-butyl formate, tert-butyl alcohol and acetone in the regulation of methyl tert-butyl ether degradation by mycobacterium austroafricanum ifp 2012.mycobacterium austroafricanum ifp 2012 is a gram-positive strain able to grow on methyl tert-butyl ether (mtbe) as a sole carbon and energy source. the effect of two downstream metabolites of mtbe, tert-butyl formate (tbf) and tert-butyl alcohol (tba) on mtbe degradation was investigated using resting cells. the addition of low concentrations of tbf decreased the mtbe degradation rate by about 30%. in contrast, the addition of tba did not have a significant effect on mtbe degradation rate, even ...200312883872
physicochemical soil parameters affecting sequestration and mycobacterial biodegradation of polycyclic aromatic hydrocarbons in soil.six soils, obtained from grasslands and wooded areas in northeastern illinois, were physicochemically characterized. measured parameters included total organic carbon (toc) content, contents of humic acid, fulvic acid and humin, pore volume and pore size distribution, and chemical makeup of soil organic matter (determined using solid-state 13c-nmr). moistened, gamma-sterilized soils were spiked with 200 ppm of either phenanthrene or pyrene (including 14c label); following 0, 40, or 120 days of a ...200312871739
classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, mycobacterium sp. strain pyr-1, as mycobacterium vanbaalenii sp. nov.a polycyclic aromatic hydrocarbon (pah)-utilizing mycobacterium strain, pyr-1(t), was isolated from petroleum-contaminated estuarine sediments and has been shown by 16s rrna gene sequencing to be closely related to mycobacterium aurum atcc 23366(t) and mycobacterium vaccae atcc 15438(t). in this investigation, the 16s rdna, fatty acid methyl esters, dna-dna hybridization, pfge analysis of restriction-digested total genomic dna and biochemical tests were used to determine the taxonomic relationsh ...200212508859
a mycobacterium strain with extended capacities for degradation of gasoline hydrocarbons.a bacterial strain (strain ifp 2173) was selected from a gasoline-polluted aquifer on the basis of its capacity to use 2,2, 4-trimethylpentane (isooctane) as a sole carbon and energy source. this isolate, the first isolate with this capacity to be characterized, was identified by 16s ribosomal dna analysis, and 100% sequence identity with a reference strain of mycobacterium austroafricanum was found. mycobacterium sp. strain ifp 2173 used an unusually wide spectrum of hydrocarbons as growth subs ...200010831416
a new rapidly growing mycobacterial species, mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre.scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children's day care centre represented a phenetically and genetically distinct group of strains. a 16s rdna dendrogram (1243 bp) showed that the closest species to the new strain ma112/96t was mycobacterium abscessus. phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with m. abscessus, mycobacterium vaccae, mycobacterium aurum and mycobacterium austroafricanum. ribot ...199910028244
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