| rhizobium ciceri sp. nov., consisting of strains that nodulate chickpeas (cicer arietinum l.). | the taxonomic status of 16 collection strains of chickpea (cicer arietinum l.) rhizobia which were previously determined to belong to two groups (groups a and b) were compared with reference strains belonging to different genera and species of the family rhizobiaceae. we used the following taxonomic, phylogenetic, and phenotypic characteristics and approaches to study these organisms: dna homology, guanine-plus-cytosine content, restriction fragment length polymorphism of the amplified 16s-inter ... | 1994 | 7520739 |
| genomic heterogeneity of strains nodulating chickpeas (cicer arietinum l.) and description of rhizobium mediterraneum sp. nov. | the genetic diversity of chickpea strains was studied by using 30 isolates obtained from nodules on chickpeas growing in uninoculated fields over a wide geographic range. the following taxonomic approaches were used: dna-dna relatedness analysis, restriction fragment length polymorphism analysis of the amplified 16s ribosomal dna (rdna) intergenic spacer (igs), and total 16s rrna sequence analysis. the division of chickpea-infective strains into two major phylogenetic groups (groups a and b) tha ... | 1995 | 7547282 |
| inducers of nod genes of rhizobium ciceri. | induction of nodabc genes of r. ciceri was studied by constructing nodabc-lacz fusion. the root exudates of the homologous hosts induced the expression of nodabc genes but those of heterologous hosts failed to do so. the hplc analysis of the root exudates of c. arietinum showed the presence of 6-7 compounds with retention times matching to flavonoids like naringenin, hesperetin, daidzein, naringin, 7 oh coumarin and luteolin. induction studies using the standard flavonoids showed naringenin, fol ... | 1999 | 10356796 |
| biochemical controls of citrate synthase in chickpea bacteroids. | bacteroids formed by mesorhizobium ciceri cc 1192 in symbiosis with chickpea plants (cicer arietinum l.) contained a single form of citrate synthase [citrate oxaloacetate-lyase (coa-acetylating) enzyme; ec 4.1.3.7], which had the same electrophoretic mobility as the enzyme from the free-living cells. the citrate synthase from cc 1192 bacteroids had a native molecular mass of 228 +/- 32 kda and was activated by kcl, which also enhanced stability. double reciprocal plots of initial velocity agains ... | 2000 | 10648103 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of mesorhizobium ciceri. | to characterize the physiological and metabolic responses of mesorhizobium ciceri strain ch-191 to salt stress, investigating the changes induced by salinity in protein and lipopolysaccharide profiles, as well as determining the accumulation of amino acids, glutamate and proline. | 2001 | 11298245 |
| rhizobial survival and nodulation of chickpea as influenced by fungicide seed treatment. | the survival of rhizobium ciceri on chickpea (cicer arietinum cv. myles) seed, treated separately with 1 of 4 commercial fungicides, i.e., apron, arrest 75w, crown, or captan, was examined under laboratory conditions using standard serial dilution and plate count techniques. the resulting effects of fungicide-rhizobium interactions on nodulation, n2 fixation, and plant growth were assessed in a controlled environment. fungicide treatment decreased the number of viable rhizobia on the seed. in ge ... | 2001 | 11467735 |
| enzymes of malate metabolism in mesorhizobium ciceri cc 1192. | electrophoretic studies were performed on enzymes concerned with the oxidation of malate in free-living and bacteroid cells of mesorhizobium ciceri cc 1192, which forms nitrogen-fixing symbioses with chickpea (cicer arietinum l.) plants. two malate dehydrogenases were detected in extracts from both types of cells in native polyacrylamide electrophoresis gels that were stained for enzyme activity. one band of malate dehydrogenase activity was stained only in the presence of nadp+, whereas the oth ... | 2002 | 12030699 |
| preincubation of mesorhizobium ciceri with flavonoids improves its nodule occupancy. | strains of m. ciceri, symbionts of chickpea (cicer arietinum) were incubated with the flavonoids naringenin, daidzein and quercetin which have earlier been reported as inducers and inhibitors of nodabc-lacz fusion of m. ciceri. preincubation of m. ciceri with naringenin and daidzein (100 nmol/l) for 1 d improved the competitive ability of the inoculated strain while preincubation with quercetin decreased the nodule occupancy of inoculated strain under sterile conditions. under non-sterile condit ... | 2002 | 12503401 |
| lipopolysaccharides from mesorhizobium huakuii and mesorhizobium ciceri: chemical and immunological comparative data. | lipopolysaccharides of two mesorhizobium species of different host specificity were compared: m. huakuii and m. ciceri. m. huakuii sp. was represented by five strains with special consideration of m. huakuii ifo 15243(t). sds/page profiles revealed that all m. huakuii lps preparations contained low molecular mass fractions (lps-ii) of the same molecular size. all of lipopolysaccharides contained high molecular mass fractions (lps-i). however, the high molecular mass fraction from each strain pos ... | 2002 | 12545212 |
| the polar lipid composition of mesorhizobium ciceri. | the extractable lipid composition of mesorhizobium ciceri strain hambi 1750 grown in a phosphate sufficient medium (79ca) is reported. cardiolipin (cl-27% of total lipids), phosphatidylglycerol (pg-18%), phosphatidylethanolamine (pe-1%), phosphatidylcholine (pc-30%) and two methylated derivatives of pe, i.e. phosphatidyl-n, n-dimethylethanolamine (dmpe-1%) and phosphatidyl-n-monomethylethanolamine (mmpe-1%), were found to make up the phospholipids of the analysed bacteria. nonphosphorus, ornithi ... | 2003 | 12633685 |
| rhizobial strain involvement in plant growth, nodule protein composition and antioxidant enzyme activities of chickpea-rhizobia symbioses: modulation by salt stress. | mesorhizobium ciceri, mesorhizobium mediterraneum and sinorhizobium medicae strains showed different symbiotic performances when inoculated to chickpea (cicer arietinum l., cv. chetoui) at unstressed conditions and under salt stress. the analysis of nodular proteic composition and antioxidant enzyme activities revealed a polymorphism of patterns on sds and native page suggesting a potential dependence on the bacterial partner. salt effect was analysed on plant growth, nitrogen fixation and antio ... | 2004 | 15474377 |
| azide resistance in rhizobium ciceri linked with superior symbiotic nitrogen fixation. | isolated azide resistant (azr) native r. ciceri strain 18-7 was resistant to sodium azide at 10 microg/ml. to find if nif-reiteration is responsible for azide resistance and linked to superior symbiotic nitrogen fixation, transposon (tn5) induced azide sensitive mutants were generated. using 4 kb nif-reiterated sinorhizobium meliloti dna, a clone c4 that complemented azide sensitivity was isolated by dna hybridization from genomic library of chickpea rhizobium strain rcd301. ecori restriction ma ... | 2004 | 15623228 |
| rhizobial 16s rrna and dnak genes: mosaicism and the uncertain phylogenetic placement of rhizobium galegae. | the phylogenetic relatedness among 12 agriculturally important species in the order rhizobiales was estimated by comparative 16s rrna and dnak sequence analyses. two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. one group consisted of mesorhizobium loti and mesorhizobium ciceri, and the other group consisted of agrobacterium rhizogenes, rhizobium tropici, rhizobium etli, and rhizobium leguminosarum. although bootstrap support for the placement of t ... | 2005 | 15746335 |
| symbiotic effectiveness of a siderophore overproducing mutant of mesorhizobium ciceri. | mutants of mesorhizobium ciceri bicc 651 were generated by n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis. siderophore overproducing mutants were identified on chrome azurol s agar plates. one of them determined as n15 was examined for symbiotic efficiency and compared to its wild type parent i.e. bicc 651 strain under sterile conditions using leonard jars in growth chamber and also in pots containing nonsterile alluvial field soil. it was observed that the strain n15 produced about 30% higher ... | 2005 | 16209093 |
| molecular phylogeny based on the 16s rrna gene of elite rhizobial strains used in brazilian commercial inoculants. | nitrogen is often a limiting nutrient, therefore the sustainability of food crops, forages and green manure legumes is mainly associated with their ability to establish symbiotic associations with stem and root-nodulating n2-fixing rhizobia. the selection, identification and maintenance of elite strains for each host are critical. decades of research in brazil resulted in a list of strains officially recommended for several legumes, but their genetic diversity is poorly known. this study aimed a ... | 2006 | 16442259 |
| screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. | plant growth promoting rhizobacteria (pgpr) are known to influence plant growth by various direct or indirect mechanisms. in search of efficient pgpr strains with multiple activities, a total of 72 bacterial isolates belonging to azotobacter, fluorescent pseudomonas, mesorhizobium and bacillus were isolated from different rhizospheric soil and plant root nodules in the vicinity of aligarh. these test isolates were biochemically characterized. these isolates were screened in vitro for their plant ... | 2008 | 16735107 |
| effect of salinity on root-nodule conductance to the oxygen diffusion in the cicer arietinum-mesorhizobium ciceri symbiosis. | nodule conductance to o2 diffusion has been involved as a major factor of the inhibition of n2 fixation by soil salinity that severely reduces the production of grain legumes. in order to determine the effect of this constraint on the nodule conductance, oxygen uptake by the nodulated roots of cicer arietinum was measured by recording the concentration of o2 as a function of po2 in a gas-tight incubator. after germination and inoculation with the strain mesorhizobium ciceri upmca7, the varieties ... | 2007 | 16904230 |
| characterization of intrinsic variability of mesorhizobium ciceri isolates of cultivated fields. | a large number of putative rhizobial isolates were obtained from the root nodules of various chickpea cultivars growing in agricultural research fields. of these, thirty were selected and characterized for traits, such as, generation time, intrinsic azide resistance and several symbiotic characters. | 2006 | 16924839 |
| characterization of a symbiotically defective serine auxotroph of mesorhizobium ciceri. | a tn5-induced mutant strain (tl68) of mesorhizobium ciceri unable to grow with ammonium as the sole nitrogen source was isolated and characterized. unlike its wild-type parent (strain tal620), the mutant had an absolute dependence on serine to grow. cloning of the dna region containing tn5 and sequence analysis showed that tn5 was inserted into the gene coding for 3-phosphoglycerate dehydrogenase, which catalyses the first step in the serine biosynthetic pathway. the role of serine biosynthesis ... | 2006 | 16978364 |
| strains of mesorhizobium amorphae and mesorhizobium tianshanense, carrying symbiotic genes of common chickpea endosymbiotic species, constitute a novel biovar (ciceri) capable of nodulating cicer arietinum. | to identify several strains of mesorhizobium amorphae and mesorhizobium tianshanense nodulating cicer arietinum in spain and portugal, and to study the symbiotic genes carried by these strains. | 2007 | 17397480 |
| mesorhizobium ciceri biovar biserrulae, a novel biovar nodulating the pasture legume biserrula pelecinus l. | biserrula pelecinus l. is a pasture legume species that forms a highly specific nitrogen-fixing symbiotic interaction with a group of bacteria that belong to mesorhizobium. these mesorhizobia have >98.8 % sequence similarity to mesorhizobium ciceri and mesorhizobium loti for the 16s rrna gene (1440 bp) and >99.3 % sequence similarity to m. ciceri for the dnak gene (300 bp), and strain wsm1271 has 100 % sequence similarity to m. ciceri for gsii (600 bp). strain wsm1271 had 85 % relatedness to m. ... | 2007 | 17473255 |
| phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of tunisia. | several phenotypic markers were used in this study to determine the biodiversity of rhizobial strains nodulating cicer arietinum l. in various areas of tunisia. they include symbiotic traits, the use of 21 biochemical substrates, and tolerance to salinity and ph. in addition, restriction fragment length polymorphisms (rflps) of pcr-amplified 16s rdna were compared with those of reference strains. numeric analysis of the phenotypic characteristics showed that the 48 strains studied fell into thre ... | 2007 | 17538653 |
| survival of bio-inoculants on fungicides-treated seeds of wheat, pea and chickpea and subsequent effect on chickpea yield. | survival of mesorhizobium ciceri (sp(4)) and azotobacter chroococcum (cbd-15 and m(4)) was tested on chickpea (cicer arietinum) seeds treated with fungicides bavistin [methyl n-(1h-benzimidazol-2yl) carbamate] and thiram (tetramethyl-thiuram disulfide), whereas survival of phosphate solubilizing bacteria (psb), pseudomonas striata (27) and bacillus polymyxa (h(5)) was examined on two cultivars (arkel and bv) of pea (pisum sativum) seeds treated with thiram. viability of azotobacter chroococcum ( ... | 2007 | 17701702 |
| in situ lateral transfer of symbiosis islands results in rapid evolution of diverse competitive strains of mesorhizobia suboptimal in symbiotic nitrogen fixation on the pasture legume biserrula pelecinus l. | the multi-billion dollar asset attributed to symbiotic nitrogen fixation is often threatened by the nodulation of legumes by rhizobia that are ineffective or poorly effective in n(2) fixation. this study investigated the development of rhizobial diversity for the pasture legume biserrula pelecinus l., 6 years after its introduction, and inoculation with mesorhizobium ciceri bv. biserrulae strain wsm1271, to western australia. molecular fingerprinting of 88 nodule isolates indicated seven were di ... | 2007 | 17803775 |
| characterization of an rpon mutant of mesorhizobium ciceri. | to study the genetic basis of c(4)-dicarboxylate transport (dct) in relation to symbiotic nitrogen fixation in mesorhizobium ciceri. | 2007 | 17953590 |
| survey of chickpea rhizobia diversity in portugal reveals the predominance of species distinct from mesorhizobium ciceri and mesorhizobium mediterraneum. | several mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering portugal as a case study. one hundred and ten isolates were obtained from continental portugal and madeira island. the 16s ribosomal rna gene phylogeny revealed that isolates are highly diverse, grouping with mos ... | 2009 | 19468700 |
| mesorhizobium shangrilense sp. nov., isolated from root nodules of caragana species. | five strains of bacteria isolated from nodules of caragana bicolor and caragana erinacea in yunnan province of china were classified within the genus mesorhizobium in the class alphaproteobacteria. the highest degree of 16s rrna gene sequence similarity was determined to be to mesorhizobium loti lmg 6125(t) (99.7 %) and mesorhizobium ciceri upm-ca7(t) (99.7 %). polyphasic taxonomic methods including sds-page of whole-cell soluble proteins, comparative housekeeping sequence analysis of atpd, glni ... | 2009 | 19643905 |
| high diversity of chickpea mesorhizobium species isolated in a portuguese agricultural region. | chickpea rhizobia isolated from portuguese soils were assigned to the genus mesorhizobium by 16s-rdna sequencing. high species diversity was found within populations of an agricultural region in the south of portugal. besides the expected mesorhizobium ciceri and m. mediterraneum, some isolates were close to m. loti or m. tianshanense and some formed a clade that may represent a new species. a new pcr-based approach, named direct amplified polymorphic dna (dapd) analysis, supported the 16s-based ... | 2004 | 19712435 |
| horizontal gene transfer and recombination shape mesorhizobial populations in the gene center of the host plants astragalus luteolus and astragalus ernestii in sichuan, china. | thirty-three rhizobial strains isolated from the root nodules of astragalus luteolus and astragalus ernestii growing on the west plateau at two different altitudes in sichuan province, china, were characterized by amplified rdna restriction analysis (ardra), amplified fragment length polymorphism (aflp), and by sequencing of rrs, glna, glnii and nifh. the ardra analysis revealed considerable genomic diversity. in aflp analysis, 20 of 33 astragalus rhizobia formed three distinct clades, with othe ... | 2009 | 19796139 |
| mutation in the lysa gene impairs the symbiotic properties of mesorhizobium ciceri. | a tn5-induced mutant of mesorhizobium ciceri, tl28, requiring the amino acid lysine for growth on minimal medium was isolated and characterized. the tn5 insertion in the mutant strain tl28 was located on a 6.8-kb ecori fragment of the chromosomal dna. complementation analysis with cloned dna indicated that 1.269 kb of dna of the 6.8-kb ecori fragment restored the wild-type phenotype of the lysine-requiring mutant. this region was further characterized by dna sequence analysis and was shown to co ... | 2010 | 20020102 |
| transformation of sinorhizobium meliloti mtcc 100 and mesorhizobium ciceri tal 620 by cacl2 method. | the cacl2 method, commonly used for transformation of escherichia coli, was modified and used to develop a simpler and easier transformation method for rhizobia sp. two species of rhizobia, sinorhizobium meliloti mtcc 100 and mesorhizobium ciceri tal 620, were transformed with the 13.2 kb binary vector pga482. at an optical density of 0.4, the transformation efficiencies in sinorhizobium meliloti mtcc 100 and mesorhizobium ciceri tal 620 were 104 and 103, respectively. competent cells of sinorhi ... | 2010 | 20130696 |
| tolerance of mesorhizobium type strains to different environmental stresses. | the symbiosis between rhizobia and legumes is affected by different environmental conditions. our aims were to evaluate stress tolerance of mesorhizobium species and investigate species-specific stress response mechanisms. tolerance of mesorhizobium type strains to temperature, salt and ph stress was evaluated. mesorhizobium thiogangeticum showed highest growth with 1.5% nacl and mesorhizobium ciceri at ph 5. mesorhizobium plurifarium showed higher growth at 37°c. sds-page analysis revealed chan ... | 2010 | 21152981 |
| development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits. | the ability of cyanobacteria to be useful as matrices for agriculturally important bacteria was evaluated. biofilms were generated with the selected strain anabaena torulosa after co-culturing with azotobacter chroococcum, pseudomonas striata, serratia marcescens, and mesorhizobium ciceri. the biochemical attributes were compared with individual bacterial and cyanobacterial cultures. the biofilms were characterized in terms of proteins, chlorophyll, iaa production, acetylene-reducing activity, p ... | 2011 | 21448713 |
| effects of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on fungal occupancy in chickpea root and nodule determined by real-time pcr. | legume roots in nature are usually colonized with rhizobia and different arbuscular mycorrhizal fungi (amf) species. light microscopy that visualizes the presence of amf in roots is not able to differentiate the ratio of each amf species in the root and nodule tissues in mixed fungal inoculation. the purpose of this study was to characterize the dominant species of mycorrhiza in roots and nodules of plants co-inoculated with mycorrhizal fungi and rhizobial strains. glomus intraradices (gi), glom ... | 2011 | 21604099 |
| characterization of rhizobia from legumes of agronomic interest grown in semi-arid areas of central spain relates genetic differences to soil properties. | a study of symbiotic bacteria from traditional agricultural legumes from central spain was performed to create a collection of rhizobia from soils differing in physicochemical, analytical and/or agroecological properties which could be well-adapted to the environmental conditions of this region, and be used for sustainable agricultural practices. thirty-six isolates were obtained from root-nodules of fifteen legume species (including cicer arietinum, lathyrus sativus, lens culinaris, lupinus spp ... | 2011 | 21953333 |
| differential response of kabuli and desi chickpea genotypes toward inoculation with pgpr in different soils. | pakistan is among top three chickpea producing countries but the crop is usually grown on marginal lands without irrigation and fertilizer application which significantly hampers its yield. soil fertility and inoculation with beneficial rhizobacteria play a key role in nodulation and yield of legumes. four kabuli and six desi chickpea genotypes were, therefore, evaluated for inoculation response with iaa-producing ochrobactrum ciceri ca-34(t) and nitrogen fixing mesorhizobium ciceri tal-1148 in ... | 2015 | 26379638 |
| phylogeny of symbiotic genes and the symbiotic properties of rhizobia specific to astragalus glycyphyllos l. | the phylogeny of symbiotic genes of astragalus glycyphyllos l. (liquorice milkvetch) nodule isolates was studied by comparative sequence analysis of noda, nodc, nodh and nifh loci. in all these genes phylograms, liquorice milkvetch rhizobia (closely related to bacteria of three species, i.e. mesorhizobium amorphae, mesorhizobium septentrionale and mesorhizobium ciceri) formed one clearly separate cluster suggesting the horizontal transfer of symbiotic genes from a single ancestor to the bacteria ... | 2015 | 26496493 |
| assembly and transfer of tripartite integrative and conjugative genetic elements. | integrative and conjugative elements (ices) are ubiquitous mobile genetic elements present as "genomic islands" within bacterial chromosomes. symbiosis islands are ices that convert nonsymbiotic mesorhizobia into symbionts of legumes. here we report the discovery of symbiosis ices that exist as three separate chromosomal regions when integrated in their hosts, but through recombination assemble as a single circular ice for conjugative transfer. whole-genome comparisons revealed exconjugants deri ... | 2016 | 27733511 |
| complete genome sequence of mesorhizobium ciceri bv. biserrulae strain wsm1284, an efficient nitrogen-fixing microsymbiont of the pasture legume biserrula pelecinus. | we report the complete genome sequence of mesorhizobium ciceri bv. biserrulae strain wsm1284, a nitrogen-fixing microsymbiont of the pasture legume biserrula pelecinus the genome consists of 6.88 mb distributed between a single chromosome (6.33 mb) and a single plasmid (0.55 mb). | 2016 | 27284134 |
| role of nodd gene product and flavonoid interactions in induction of nodulation genes in mesorhizobium ciceri. | mesorhizobium ciceri is a host specific bacterium which nodulates the genus, cicer. host specificity is regulated at first step by induction of nodulation (nod) genes in the presence of nodd protein and inducers (flavonoids) of plant origin. the inducer specificity of m. ciceri nodd gene was studied in nodd-mutant strain hn-9 carrying heterologous nodd genes and nodalacz fusion. the induction profile of nod promoter in m. ciceri revealed that nodd gene product of m. ciceri is specifically activa ... | 2010 | 23572956 |
| development of mesorhizobium ciceri-based biofilms and analyses of their antifungal and plant growth promoting activity in chickpea challenged by fusarium wilt. | biofilmed biofertilizers have emerged as a new improved inoculant technology to provide efficient nutrient and pest management and sustain soil fertility. in this investigation, development of a trichoderma viride-mesorhizobium ciceri biofilmed inoculant was undertaken, which we hypothesized, would possess more effective biological nitrogen fixing ability and plant growth promoting properties. as a novel attempt, we selected mesorhizobium ciceri spp. with good antifungal attributes with the assu ... | 2017 | 28148979 |
| multilocus sequence analysis supports the taxonomic position of astragalus glycyphyllos symbionts based on dna-dna hybridization. | in this study, the phylogenetic relationship and taxonomic status of six strains, representing different phenons and genomic groups of astragalus glycyphyllos symbionts, originating from poland, were established by comparative analysis of five concatenated housekeeping gene sequences (atpd, dnak, glna, reca and rpob), dna-dna hybridization and total dna g+c content. maximum-likelihood phylogenetic analysis of combined atpd, dnak, glna, reca and rpob sequence data placed the studied bacteria into ... | 2016 | 26704062 |
| mesorhizobium waimense sp. nov. isolated from sophora longicarinata root nodules and mesorhizobium cantuariense sp. nov. isolated from sophora microphylla root nodules. | in total 14 strains of gram-stain-negative, rod-shaped bacteria were isolated from sophora longicarinata and sophora microphylla root nodules and authenticated as rhizobia on these hosts. based on the 16s rrna gene phylogeny, they were shown to belong to the genus mesorhizobium, and the strains from s. longicarinata were most closely related to mesorhizobium amorphae accc 19665t (99.8–99.9 %), mesorhizobium huakuii iam 14158t (99.8–99.9 %), mesorhizobium loti usda 3471t (99.5–99.9 %) and mesorhi ... | 2015 | 26296780 |
| complete genome sequence of mesorhizobium australicum type strain (wsm2073(t)). | mesorhizobium australicum strain wsm2073(t) was isolated from root nodules on the pasture legume biserrula pelecinus growing in australia in 2000. this aerobic, motile, gram negative, non-spore-forming rod is poorly effective in n2 fixation on b. pelecinus and has gained the ability to nodulate b. pelecinus following in situ lateral transfer of a symbiosis island from the original inoculant strain for this legume, mesorhizobium ciceri bv. biserrulae wsm1271. we describe that the genome size of m ... | 2013 | 24976896 |
| mesorhizobium sangaii sp. nov., isolated from the root nodules of astragalus luteolus and astragalus ernestii. | our previous published data indicated that the two rhizobial strains scau7(t) and scau27, which were isolated from the root nodules of astragalus luteolus and astragalus ernestii respectively, in sichuan province, china, might be novel species of the genus mesorhizobium. their exact taxonomic position was determined in the present study by using polyphasic approaches. comparative analysis of nearly full-length 16s rrna gene sequences showed that these strains belonged to the genus mesorhizobium, ... | 2013 | 23315406 |
| mesorhizobium qingshengii sp. nov., isolated from effective nodules of astragalus sinicus. | in a study on the diversity of rhizobia isolated from root nodules of astragalus sinicus, five strains showed identical 16s rrna gene sequences. they were related most closely to the type strains of mesorhizobium loti, mesorhizobium shangrilense, mesorhizobium ciceri and mesorhizobium australicum, with sequence similarities of 99.6-99.8%. a polyphasic approach, including 16s-23s intergenic spacer (igs) rflp, comparative sequence analysis of 16s rrna, atpd, glnii and reca genes, dna-dna hybridiza ... | 2013 | 23041644 |
| synergistic effect of chickpea plants and mesorhizobium as a natural system for chromium phytoremediation. | the presence of chromium in soils not only affects the physiological processes of plants but also the microbial rhizosphere composition and metabolic activities of microorganisms. hence, the inoculation of plants with cr(vi)-tolerant rhizospheric microorganisms as an alternative to reduce cr phytotoxicity was studied. in this work, chickpea germination was reduced by cr(vi) concentrations of 150 and 250 mg/l (6 and 33%, respectively); however lower cr(vi) concentrations negatively affected the b ... | 2016 | 27788623 |
| adaptation of the symbiotic mesorhizobium-chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism. | low inorganic phosphate (pi) availability is a major constraint for efficient nitrogen fixation in legumes, including chickpea. to elucidate the mechanisms involved in nodule acclimation to low pi availability, two mesorhizobium-chickpea associations exhibiting differential symbiotic performances, mesorhizobium ciceri cp-31 (mccp-31)-chickpea and mesorhizobium mediterranum swri9 (mmswri9)-chickpea, were comprehensively studied under both control and low pi conditions. mmswri9-chickpea showed a l ... | 2016 | 27450089 |
| dissecting the root nodule transcriptome of chickpea (cicer arietinum l.). | a hallmark trait of chickpea (cicer arietinum l.), like other legumes, is the capability to convert atmospheric nitrogen (n2) into ammonia (nh3) in symbiotic association with mesorhizobium ciceri. however, the complexity of molecular networks associated with the dynamics of nodule development in chickpea need to be analyzed in depth. hence, in order to gain insights into the chickpea nodule development, the transcriptomes of nodules at early, middle and late stages of development were sequenced ... | 2016 | 27348121 |
| complete genome sequence of mesorhizobium ciceri strain cc1192, an efficient nitrogen-fixing microsymbiont of cicer arietinum. | we report the complete genome sequence of mesorhizobium ciceri strain cc1192, an efficient nitrogen-fixing microsymbiont of cicer arietinum (chickpea). the genome consists of 6.94 mb distributed between a single chromosome (6.29 mb) and a plasmid (0.65 mb). | 2016 | 27284135 |
| genetic diversity and distribution of rhizobia associated with the medicinal legumes astragalus spp. and hedysarum polybotrys in agricultural soils. | with the increasing cultivation of medicinal legumes in agricultural fields, the rhizobia associated with these plants are facing new stresses, mainly from fertilization and irrigation. in this study, investigations on the nodulation of three cultivated medicinal legumes, astragalus mongholicus, astragalus membranaceus and hedysarum polybotrys were performed. bacterial isolates from root nodules of these legumes were subjected to genetic diversity and multilocus sequence analyses. in addition, t ... | 2016 | 26915496 |
| mesorhizobium calcicola sp. nov., mesorhizobium waitakense sp. nov., mesorhizobium sophorae sp. nov., mesorhizobium newzealandense sp. nov. and mesorhizobium kowhaii sp. nov. isolated from sophora root nodules in new zealand. | in total, 31 strains of gram-negative, rod-shaped bacteria were isolated from sophora root nodules and authenticated as rhizobia on this hos. based on the 16s rrna gene phylogeny, they were shown to belong to the genus mesorhizobium, with the representative strains icmp 19560t, icmp 19523t, icmp 19535t, icmp 19545t and icmp 19512t being most closely related to mesorhizobium sangaii scau7t (99.9 - 99.6%), mesorhizobium cantuariense icmp 19515t (99.7 - 99.6%) and mesorhizobium ciceri ump-ca7t (99. ... | 2015 | 26610329 |
| diverse novel mesorhizobia nodulate new zealand native sophora species. | forty eight rhizobial isolates from new zealand (nz) native sophora spp. growing in natural ecosystems were characterised. thirty eight isolates across five groups showed greatest similarity to mesorhizobium ciceri lmg 14989(t) with respect to their 16s rrna and concatenated reca, glnll and rpob sequences. seven isolates had a 16s rrna sequence identical to m. amorphae atcc 19665(t) but showed greatest similarity to m. septentrionale lmg 23930(t) on their concatenated reca, glnll and rpob sequen ... | 2015 | 25498849 |
| complete genome sequence of mesorhizobium ciceri bv. biserrulae type strain (wsm1271(t)). | mesorhizobium ciceri bv. biserrulae strain wsm1271(t) was isolated from root nodules of the pasture legume biserrula pelecinus growing in the mediterranean basin. previous studies have shown this aerobic, motile, gram negative, non-spore-forming rod preferably nodulates b. pelecinus - a legume with many beneficial agronomic attributes for sustainable agriculture in australia. we describe the genome of mesorhizobium ciceri bv. biserrulae strain wsm1271(t) consisting of a 6,264,489 bp chromosome a ... | 2014 | 25197432 |
| genotypic alteration and competitive nodulation of mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils. | mesorhizobium muleiense, mesorhizobium mediterraneum and mesorhizobium ciceri are chickpea (cicer arietinum l.) rhizobia that share a high similarity of the symbiotic genes nodc and nifh, but they have different geographic distributions. m. muleiense has been isolated and found only in alkaline soils of xinjiang, china, whereas the other two strains have been found in the mediterranean and india. to investigate the species stability of m. muleiense during natural evolution and its capability of ... | 2014 | 25123757 |
| complete genome sequence of mesorhizobium opportunistum type strain wsm2075(t.). | mesorhizobium opportunistum strain wsm2075(t) was isolated in western australia in 2000 from root nodules of the pasture legume biserrula pelecinus that had been inoculated with m. ciceri bv. biserrulae wsm1271. wsm2075(t) is an aerobic, motile, gram negative, non-spore-forming rod that has gained the ability to nodulate b. pelecinus but is completely ineffective in n2 fixation with this host. this report reveals that the genome of m. opportunistum strain wsm2075(t) contains a chromosome of size ... | 2013 | 24976886 |
| mechanisms of physiological adjustment of n2 fixation in cicer arietinum l. (chickpea) during early stages of water deficit: single or multi-factor controls. | drought negatively impacts symbiotic nitrogen fixation (snf) in cicer arietinum l. (chickpea), thereby limiting yield potential. understanding how drought affects chickpea nodulation will enable the development of strategies to biotechnologically engineer chickpea varieties with enhanced snf under drought conditions. by analyzing carbon and nitrogen metabolism, we studied the mechanisms of physiological adjustment of nitrogen fixation in chickpea plants nodulated with mesorhizobium ciceri during ... | 2014 | 24947137 |
| isolation and characterization of symbiotically defective pyrimidine and amino acid auxotroph of mesorhizobium ciceri in chickpea. | transposon tn5 induced, four mesorhizobium ciceri auxotroph were isolated and characterized. unlike its wild type parent (tl 620), all four mutants were found defective for amino acid and pyramiding biosynthesis. the auxotroph mutants were characterized and found tl130 as cytosine and uracil, tl 196 for guanine, cytocine, uracil and riboflavin, tl 141 as serine and tl 38 as argentine defective. symbiotic characterization of these mutants revealed phenotypic deformities and deficiencies in biolog ... | 2013 | 24640259 |
| assessment of molecular diversity in chickpea (cicer arietinum l.) rhizobia and structural analysis of 16s rdna sequences from mesorhizobium ciceri. | molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (ssr) and 16s rdna-rflp markers. phenotypic characterization with special reference to salinity and ph tolerance was performed. these isolates were identified as different strains of mesorhizobium, rhizobium, bradyrhizobium, and agrobacterium. twenty ssr loci of mesorhizobium ciceri, distributed across the other rhizobial genome, clearly differentiated 19 rhizobial isolates. analogous c ... | 2013 | 24459830 |
| approaches for enhancement of n₂ fixation efficiency of chickpea (cicer arietinum l.) under limiting nitrogen conditions. | chickpea (cicer arietinum) is an important pulse crop in many countries in the world. the symbioses between chickpea and mesorhizobia, which fix n₂ inside the root nodules, are of particular importance for chickpea's productivity. with the aim of enhancing symbiotic efficiency in chickpea, we compared the symbiotic efficiency of c-15, ch-191 and cp-36 strains of mesorhizobium ciceri in association with the local elite chickpea cultivar 'bivanij' as well as studied the mechanism underlying the im ... | 2014 | 24267445 |
| expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in mesorhizobium spp. reduces the negative effects of salt stress in chickpea. | our goal was to study the symbiotic performance of two mesorhizobium ciceri strains, transformed with an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene (acds), in chickpea plants under salinity stress. the ee-7 (salt-sensitive) and g-55 (salt-tolerant) m. ciceri strains were transformed with an acds gene present on plasmid prkacc. salinity significantly reduced the overall growth of plants inoculated with either wild-type strains. although the growth of plants inoculated with either ... | 2013 | 24152202 |
| a clpb chaperone knockout mutant of mesorhizobium ciceri shows a delay in the root nodulation of chickpea plants. | several molecular chaperones are known to be involved in bacteria stress response. to investigate the role of chaperone clpb in rhizobia stress tolerance as well as in the rhizobia-plant symbiosis process, the clpb gene from a chickpea microsymbiont, strain mesorhizobium ciceri lms-1, was identified and a knockout mutant was obtained. the clpb knockout mutant was tested to several abiotic stresses, showing that it was unable to grow after a heat shock and it was more sensitive to acid shock than ... | 2012 | 23134119 |
| acc deaminase genes are conserved among mesorhizobium species able to nodulate the same host plant. | rhizobia strains expressing the enzyme 1-aminocyclopropane-1-carboxylate (acc) deaminase have been reported to display an augmented symbiotic performance as a consequence of lowering the plant ethylene levels that inhibit the nodulation process. genes encoding acc deaminase (acds) have been studied in rhizobium spp.; however, not much is known about the presence of acds genes in mesorhizobium spp. the aim of this study was to assess the prevalence and phylogeny of acds genes in mesorhizobium str ... | 2012 | 22846039 |
| multilocus sequence analysis reveals multiple symbiovars within mesorhizobium species. | the genus mesorhizobium includes species nodulating several legumes, such as chickpea, which has a high agronomic importance. chickpea rhizobia were originally described as either mesorhizobium ciceri or m. mediterraneum. however, rhizobia able to nodulate chickpea have been shown to belong to several different species within the genus mesorhizobium. the present study used a multilocus sequence analysis approach to infer a high resolution phylogeny of the genus mesorhizobium and to confirm the e ... | 2012 | 22817876 |
| mesorhizobium ciceri lms-1 expressing an exogenous 1-aminocyclopropane-1-carboxylate (acc) deaminase increases its nodulation abilities and chickpea plant resistance to soil constraints. | our goal was to understand the symbiotic behaviour of a mesorhizobium strain expressing an exogenous 1-aminocyclopropane-1-carboxylate (acc) deaminase, which was used as an inoculant of chickpea (cicer arietinum) plants growing in soil. | 2012 | 22486441 |