| interaction of linear manno-oligosaccharides with three mannose-specific bulb lectins. comparison with mannose/glucose-binding lectins. | three new mannose-binding lectins, isolated from daffodil (npa), amaryllis (hha), and snowdrop (gna) bulbs, are capable of precipitating with a linear mannopentaose (man alpha 1-3man alpha 1-3man alpha 1-3man alpha 1-2man). npa and hha reacted strongly with the mannopentaose whereas gna gave a precipitate only at concentrations greater than 500 microm. a phosphate group at c-6 of the nonreducing terminal mannosyl group prevented precipitation in all three cases. the reduced (nabh4) mannopentaose ... | 1992 | 1394290 |
| alpha-(1-3)- and alpha-(1-6)-d-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitro. | the alpha-(1-3)-d-mannose- and alpha-(1-6)-d-mannose-specific agglutinins (lectins) from galanthus nivalis, hippeastrum hybrid, narcissus pseudonarcissus, and listera ovata inhibited infection of mt-4 cells by human immunodeficiency virus types 1 and 2 (hiv-1 and hiv-2) and simian immunodeficiency virus at concentrations comparable to the concentrations at which dextran sulfate (molecular weight, 5,000 [ds-5000]) inhibits these viruses (50% effective concentration, 0.2 to 0.6 microgram/ml). unli ... | 1991 | 1645507 |
| human immunodeficiency virus: novel enzyme-linked immunoassays for quantitation of envelope glycoprotein 120. | two novel enzyme-linked immunoassays (elisa) for the quantitation of human immunodeficiency virus type 1 (hiv-1) coded glycoprotein with an mr 120 (gp120) are described. these are based on the highly specific interaction between gp120 and the mannose-specific lectins from narcissus pseudonarcissus (npl) and galanthus nivalis (gnl). two systems were developed: (1) an hiv-protein elisa using hiv-protein (also containing hiv-gp120) for the solid phase and npl as a detector and (2) a lectin-elisa us ... | 1991 | 1874921 |
| interactions of five d-mannose-specific lectins with a series of synthetic branched trisaccharides. | the interaction of a series of synthetic, branched trisaccharides with five d-mannose-specific lectins was studied by precipitation-inhibition assay. the branched methyl alpha-d-mannotrioside, alpha-d-manp-(1----3)-[alpha-d-manp-(1----6)]-alpha-d-man pome, the best inhibitor of the con a-dextran interaction, was 42 times more potent than alpha-d-manpome, and 3-6 times more potent than the two trisaccharides substituted with d-glucosyl groups, and 8-15 times those with d-galactosyl groups. surpri ... | 1991 | 1933932 |
| sulphoevernan, a polyanionic polysaccharide, and the narcissus lectin potently inhibit human immunodeficiency virus infection by binding to viral envelope protein. | sulphoevernan is a sulphated alpha-1----3, 1----4 polyglucan (mr 20,000) with a helical structure. this compound effectively inhibits both human immunodeficiency virus type 1 (hiv-1) and type 2 infection of cells in vitro at concentrations around 0.5 micrograms/ml. moreover, the compound completely inhibits hiv-1-induced syncytium formation at a concentration of 1 microgram/ml. competition experiments with 35s-labelled sulphoevernan revealed that the mannose-specific lectin from narcissus pseudo ... | 1990 | 2212988 |
| carbohydrate-binding specificity of the daffodil (narcissus pseudonarcissus) and amaryllis (hippeastrum hybr.) bulb lectins. | the carbohydrate binding specificity of the daffodil (narcissus pseudonarcissus; npa) and amaryllis (hippeastrum hybr.; hha) lectins, isolated from extracts of their bulbs by affinity chromatography on immobilized mannose, was studied by quantitative precipitation, sugar hapten inhibition, and affinity chromatography on the immobilized lectins. these lectins gave strong precipitation reactions with several yeast mannans, but did not precipitate with alpha-d-glucans (e.g., dextrans and glycogen). ... | 1990 | 2350177 |
| an investigation of the irritant and allergenic properties of daffodils (narcissus pseudonarcissus l., amaryllidaceae). a review of daffodil dermatitis. | irritancy of daffodil flowers and bulbs was assessed using various fresh plant preparations, solvent extracts and some of the known amaryllidaceae alkaloids on guinea pigs. sensitization was also carried out on guinea pigs using these plant preparations, solvent extracts and 7 fractions obtained after preparative chromatography of the bulb ether extract. only 1 fraction, containing 2 alkaloids, was capable of inducing delayed hypersensitivity in the animals; the sensitivity achieved, however, wa ... | 1988 | 2972509 |
| inhibition of herpes simplex, rabies and rubella viruses by lectins with different specificities. | a series of eleven lectins were investigated for their inhibiting activity towards herpes simplex type 1, rabies and rubella viruses. herpes simplex virus adsorption was inhibited by limulus polyphemus (lpa), canavalia ensiformis (cona) and wheat germ (wga) agglutinins, whereas bauhinia purpurea (bpa) and soybean agglutinins were more effective if present during viral replication; rabies virus attachment to susceptible cells was prevented by lpa and narcissus pseudonarcissus (npa) agglutinin whe ... | 1995 | 7481093 |
| glycoprotein and ganglioside changes in human trophoblasts after exposure to pulsed doppler ultrasound. | changes in glycoprotein and ganglioside composition in human trophoblasts (eighth week of gestation) after in vitro exposure to pulsed doppler ultrasound (pulse duration 1.22 microseconds; repetition frequency 11.1 khz; center frequency 4 mhz; isppa = 175.5 w/cm2; ispta = 0.59 w/cm2) were investigated. evacuated trophoblasts were divided in two halves and insonated for 10 min on top of a 6-cm layer of 5% gelatin in 50-ml tubes (falcon) at 37 degrees c. one half of each trophoblast was sham inson ... | 1995 | 7571151 |
| mannose-specific lectins bind alpha-2-macroglobulin and an unknown protein from human plasma. | gna, the mannose-specific lectin from galanthus nivalis was confirmed to bind alpha-2-macroglobulin (a2m) but another protein was copurified with a2m from total human plasma. a total of 23 other lectins with diverse specificities were tested for reaction with human a2m and with three other members of the a2m family. npa, a mannose-specific lectin isolated from narcissus pseudonarcissus bulbs, and rsa, the rhizoctonia solani agglutinin, were selected for further testing. for isolation of a2m, imm ... | 1993 | 7680268 |
| [serious plant poisonings in switzerland 1966-1994. case analysis from the swiss toxicology information center]. | to analyze the types, frequency and severity of plant poisonings in switzerland over 29 years. | 1996 | 8711457 |
| increase of glycocalyx and altered lectin agglutination profiles of pasteurella haemolytica a1 after incubation in bovine subcutaneous tissue chambers in vivo or in ruminant serum in vitro. | pasteurella haemolytica serotype a1 (bovine strain ok) was incubated for 2 and 6 h in bovine subcutaneous tissue chambers in vivo, and ovine strain 82-25 and bovine strain l011 were incubated in vitro for 2 h in heat-inactivated ovine or bovine serum from which gamma globulin had been depleted by protein g affinity chromatography to assess changes in morphology and lectin agglutination profiles (strains 82-25 and l101 only). cells, removed from chambers after 2 h, were covered with an extensive, ... | 1997 | 9038303 |
| isolation and characterization of a mannose-binding lectin from leaves of the chinese daffodil narcissus tazetta. | a mannose-binding lectin was isolated from leaves of the chinese daffodil narcissus tazetta (family amaryllidaceae) using a procedure that comprised extraction with aqueous buffer, ammonium sulfate precipitation, ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel and mannose-agarose, and fplc-gel filtration on superose 12. the lectin was adsorbed on mannose-agarose and unadsorbed on deae-cellulose and affi-gel blue gel. it was an unglycosylated homodimer ... | 1998 | 10099780 |
| effects of lectins with different carbohydrate-binding specificities on hepatoma, choriocarcinoma, melanoma and osteosarcoma cell lines. | the effects of lectins with different carbohydrate-binding specificities on human hepatoma (h3b), human choriocarcinoma (jar), mouse melanoma (b16) and rat osteosarcoma (ros) cell lines were investigated. cell viability was estimated by uptake of crystal violet. wheat germ lectin was the lectin with the most deleterious effect on the viability of h3b, jar and ros cell lines. the cytotoxicity of lectins with similar sugar-binding specificity to wheat germ lectin, including maackia amurensis lecti ... | 2000 | 10716633 |
| lectins from bulbs of the chinese daffodil narcissus tazetta (family amaryllidaceae). | the isolation of three lectins with similar n-terminal amino acid sequences from the bulbs of the chinese daffodil narcissus tazetta was achieved. the isolation protocol involved ion exchange chromatography on deae-cellulose, affinity chromatography on mannose-agarose, and fast protein liquid chromatography-gel filtration on superose 12. the lectins were all adsorbed on mannose-agarose and demonstrated a single band with a molecular weight of 13 kda in sds-polyacrylamide gel electrophoresis and ... | 2000 | 11012085 |
| biosynthesis of beta-carotene (provitamin a) in rice endosperm achieved by genetic engineering. | to obtain a functioning provitamin a (beta-carotene) biosynthetic pathway in rice endosperm, we introduced in a single, combined transformation effort the cdnas coding for (1) phytoene synthase (psy) and (2) lycopene beta-cyclase (beta-lcy; both from narcissus pseudonarcissus and both under control of the endosperm-specific glutelin promoter), with (3) a bacterial phytoene desaturase (crti, from erwinia uredovora under constitutive 35s promoter control). this combination covers the requirements ... | 2001 | 11387982 |
| golden rice: introducing the beta-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin a deficiency. | to obtain a functioning provitamin a (beta-carotene) biosynthetic pathway in rice endosperm, we introduced in a single, combined transformation effort the cdna coding for phytoene synthase (psy) and lycopene beta-cyclase (beta-lcy) both from narcissus pseudonarcissus and both under the control of the endosperm-specific glutelin promoter together with a bacterial phytoene desaturase (crti, from erwinia uredovora under constitutive 35s promoter control). this combination covers the requirements fo ... | 2002 | 11880581 |
| the potentially insecticidal narcissus pseudonarcissus lectin demonstrates age-related mitogenicity. | lectins from monocotyledonous plants such as narcissus pseudonarcissus (npa) possess insecticidal properties and have the potential to increase pest resistance in transgenic crops. therefore it is of interest to investigate the mitogenic properties of such lectins. mononuclear cells purified from human umbilical cord and adult peripheral blood samples were stimulated with npa and compared to phytohaemagglutinin as an example of a lectin from a dicotyledonous plant. here we report that npa is sli ... | 2002 | 11985968 |
| accumulation of plant small heat-stress proteins in storage organs. | plant small heat-stress proteins (shsps) have been shown to be expressed not only after exposure to elevated temperatures, but also at particular developmental stages such as embryogenesis, microsporogenesis, and fruit maturation. this paper presents new data on the occurrence of shsps in vegetative tissues, their tissue-specific distribution, and cellular localization. we have found shsps in 1-year-old twigs of acer platanoides l. and sambucus nigra l. and in the liana aristolochia macrophylla ... | 2002 | 12029471 |
| anti-human immunodeficiency virus type 1 (hiv-1) activity of lectins from narcissus species. | mannose-specific lectins (msls) were isolated from bulbs of fifteen wild narcissus species growing in spain and assayed for their hiv-1 infection inhibitory activity in mt-4 cells and compared to the narcissus pseudonarcissus agglutinin (npa), the commercially available msl obtained from daffodils. almost all the tested msls were found to be active, showing ec50 values (microg/ml) similar to that of npa, with some being comparable to those obtained with dextran sulfate without significant cytoto ... | 2003 | 12624813 |
| effects of lycorine on growth and effects of l-galactonic acid-gamma-lactone on ascorbic acid biosynthesis in strains of cryptococcus laurentii isolated from narcissus pseudonarcissus roots and bulbs. | the alkaloid lycorine, which is considered to inhibit the last step in ascorbic acid biosynthesis, is produced by narcissus pseudonarcissus. the growth of two strains (c1 and c3) of cryptococcus laurentii isolated from root tips of n. pseudonarcissus is inhibited by lycorine, as is the in vivo production of ascorbic acid from l-galactonic acid-gamma-lactone. in contrast, c. laurentii strain c4, isolated from the lycorine-containing bracts of the bulb, was not inhibited by lycorine and did not co ... | 2003 | 12755480 |
| early steps of deoxyxylulose phosphate pathway in chromoplasts of higher plants. | 1-deoxy-d-xylulose 5-phosphate and 2c-methyl-d-erythritol 4-phosphate have been shown as intermediates of the deoxyxylulose phosphate pathway used for terpenoid biosynthesis in plants and many microorganisms. in plants this non-mevalonate pathway is located in plastids. in order to investigate the formation of five carbon intermediates, chromoplasts from capsicum annuum and narcissus pseudonarcissus were incubated with isotope-labeled 1-deoxy-d-xylulose 5-phosphate or 2c-methyl-d-erythritol 4-ph ... | 2003 | 12946418 |
| daffodil flowers delay senescence in cut iris flowers. | visible symptoms of tepal senescence in cut iris x hollandica (cv. blue magic) flowers were delayed by placing one cut daffodil flower (narcissus pseudonarcissus, cv. carlton) in the same vase. addition of mucilage, exuded by daffodil stems, to the vase water had the same effect as the flowering daffodil stem. the active compound in the mucilage was identified as narciclasine (using lc/ms, gc/ms, 1h and 13c-nmr, and comparison with an authentic sample of narciclasine). the delay of senescence, e ... | 2004 | 15003420 |
| daffodil toxicosis in an adult cat. | a domestic longhair cat with a 3-day history of lethargy and vomiting after ingesting dried daffodil stems (narcissus spp.) was severely hypothermic (33.0 degrees c), with bradycardia (78 beats/ min) and hypotension. treatment with atropine, dexamethasone, fluid therapy, and supportive care resulted in a complete recovery by 6 days after exposure. | 2004 | 15072199 |
| lectin histochemistry of normal human lung. | this study aimed to identify and specify the glycotypes of cell populations in normal human lung including types i and ii pneumocytes, alveolar macrophages and mast cells, and also in the larger tissue structures of lung, including blood vessels and bronchi/bronchioles, using lectin- and immuno-histochemistry on paraffin-embedded tissue from 11 normal cases. the alveolar macrophages were anti-cd68 positive whereas the cells lining the alveolar walls were positive for cytokeratins. the alveolar m ... | 2004 | 15328919 |
| first report of a glutamine-rich antifungal peptide with immunomodulatory and antiproliferative activities from family amaryllidaceae. | this represents the first report of purification of a glutamine-rich antifungal peptide from family amarylliaceace. the peptide, designated as nartazin, was purified from the bulbs of the chinese daffodil narcissus tazetta var. chinensis by means of ion-exchange chromatography and affinity chromatography. its molecular mass was 7.1kda, as determined by sds-page and gel filtration. nartazin stimulated proliferation of mouse splenocytes and bone marrow cells but inhibited proliferation of leukemia ... | 2004 | 15522215 |
| improving the nutritional value of golden rice through increased pro-vitamin a content. | "golden rice" is a variety of rice engineered to produce beta-carotene (pro-vitamin a) to help combat vitamin a deficiency, and it has been predicted that its contribution to alleviating vitamin a deficiency would be substantially improved through even higher beta-carotene content. we hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to develop golden rice, was the limiting step in beta-carotene accumulation. through systematic testing of other plant ... | 2005 | 15793573 |
| plant development scores from fixed-date photographs: the influence of weather variables and recorder experience. | in 1944, john willis produced a summary of his meticulous record keeping of weather and plants over the 30 years 1913-1942. this publication contains fixed-date, fixed-subject photography taken on the 1st of each month from january to may, using as subjects snowdrop galanthus nivalis, daffodil narcissus pseudo-narcissus, horse chestnut aesculus hippocastanum and beech fagus sylvatica. we asked 38 colleagues to assess rapidly the plant development in each of these photographs according to a suppl ... | 2006 | 16402207 |
| narcissus symptomless virus: a new carlavirus of daffodils. | a filamentous virus, with particles 600-650 nm long, was purified from narcissus pseudonarcissus (daffodil) in hangzhou and an antiserum prepared. after mechanical inoculation, the virus could be detected serologically in narcissus species but not in some commonly used virus indicators. infection was symptomless. the complete sequence of the genomic rna (8281 nt) showed six predicted orfs typical of carlaviruses. pairwise comparisons of gene sequences and phylogenetic analysis demonstrated that ... | 2006 | 16763730 |
| carbohydrate-binding agents efficiently prevent dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (dc-sign)-directed hiv-1 transmission to t lymphocytes. | exposure of hiv-1 to dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (dc-sign)-expressing b-lymphoblast raji cells (raji/dc-sign) but not to wild-type raji/0 cells results in the capture of hiv-1 particles to the cells as measured by the quantification of cell-associated p24 antigen. cocultivation of hiv-1-captured raji/dc-sign cells with uninfected cd4+ t lymphocyte c8166 cells results in abundant formation of syncytia within 36 h after cocultivation. short preexp ... | 2007 | 17056872 |
| a novel tetrameric lectin from lycoris aurea with four mannose binding sites per monomer. | the mannose-binding agglutinin from bulbs of lycoris aurea (laa) agglutinates rabbit but not human erythrocytes. the molecular mass of the monomer in sds/page is 12 kda while the apparent molecular mass in gel filtration is 48 kda, indicating that laa is a homotetramer. the full-length cdna of laa contains 683 bp with an open reading frame encoding a protomer of 162 amino-acid residues. hydrophobic cluster analysis and molecular modeling of the 109-residue mature polypeptide suggested a similar ... | 2007 | 17356714 |
| functional analysis of a ring domain ankyrin repeat protein that is highly expressed during flower senescence. | a gene encoding a ring zinc finger ankyrin repeat protein (mjxb3), a putative e3 ubiquitin ligase, is highly expressed in petals of senescing four o'clock (mirabilis jalapa) flowers, increasing >40,000-fold during the onset of visible senescence. the gene has homologues in many other species, and the petunia homologue is strongly up-regulated in senescing petunia corollas. silencing the expression of this gene in petunia, using virus-induced gene silencing, resulted in a 2 d extension in flower ... | 2007 | 18057040 |
| isolation, characterization, molecular cloning and modeling of a new lipid transfer protein with antiviral and antiproliferative activities from narcissus tazetta. | a fetuin-binding peptide with a molecular mass of about 9kda (designated ntp) was isolated and purified from the bulbs of chinese daffodil, narcissus tazetta var. chinensis l., by gel filtration and high-performance liquid chromatography, after removing the mannose-binding proteins by mannose-agarose column. molecular cloning revealed that ntp contained an open reading frame of 354bp encoding a polypeptide of 118 amino acids which included a 26-amino-acid signal peptide. an analysis of the deduc ... | 2008 | 18824058 |
| evaluation of hot water treatments for management of ditylenchus dipsaci and fungi in daffodil bulbs. | treatment of daffodil (narcissus pseudonarcissus) bulbs in a 0.37% formaldehyde water solution at 44 c for 240 minutes is a standard practice in california for management of the stem and bulb nematode, ditylenchus dipsaci. recent concern over the safety of formaldehyde and growers' requests for a shorter treatment time prompted a reevaluation of the procedure. the time (y, in minutes) required to raise the temperature at the bulb center from 25 to 44 c was related to bulb circumference (x, in cm ... | 1993 | 19279827 |
| enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin a conversion. | carotenoids are essential pigments of the photosynthetic apparatus and an indispensable component of the human diet. in addition to being potent antioxidants, they also provide the vitamin a precursor beta-carotene. in tomato (solanum lycopersicum) fruits, carotenoids accumulate in specialized plastids, the chromoplasts. how the carotenoid biosynthetic pathway is regulated and what limits total carotenoid accumulation in fruit chromoplasts is not well understood. here, we have introduced the lyc ... | 2009 | 19587100 |
| first report of mucor circinelloides occurring on yellow catfish (pelteobagrus fulvidraco) from china. | infected yellow catfish (pelteobagrus fulvidraco) were sent from niushan lake fishery, hubei province, china, to our laboratory for diagnosis. macroscopic daffodil yellow mold was observed on the heads and fins of the fish and one mucor species was isolated. based on the morphological and molecular analysis, the species was identified as mucor circinelloides. its optimum growth temperature was 30 degrees c and it could not grow at 40 degrees c. the infectivity results showed wound infection coul ... | 2010 | 19930461 |
| narcissus tazetta lectin shows strong inhibitory effects against respiratory syncytial virus, influenza a (h1n1, h3n2, h5n1) and b viruses. | a mannose-binding lectin (narcissus tazetta lectin [ntl]) with potent antiviral activity was isolated and purified from the bulbs of the chinese daffodil narcissus tazetta var. chinensis, using ion exchange chromatography on diethylaminoethyl (deae)-cellulose, affinity chromatography on mannose-agarose and fast protein liquid chromatography (fplc)-gel filtration on superose 12. the purified lectin was shown to have an apparent molecular mass of 26 kda by gel filtration and 13 kda by sds-page, in ... | 2010 | 20413914 |
| dynamic electromagnetophoretic force analysis of a single binding interaction between lectin and mannan polysaccharide on yeast cell surface. | using the electromagnetophoretic buoyancy for a microparticle in a silica capillary containing an electrolyte solution, the dynamic force dissociation kinetics of the interaction between the mannan polysaccharide on a yeast cell surface and lectins bound to the silica capillary wall have been studied by using the two different modes of the increasing force mode and the constant force mode. lectins used in the study were concanavalin a (con a), hippeastrum hybrid lectin (hhl), galanthus nivalis l ... | 2010 | 20498887 |
| human cytochrome p450 liability studies of trans-dihydronarciclasine: a readily available, potent, and selective cancer cell growth inhibitor. | the cytochrome p45o activities of the naturally occurring amaryllidaceae alkaloid narciclasine (3), isolated from narcissus pseudonarcissus, and synthetic derivative trans-dihydronarciclasine (5) are reported. while narciclasine was found to possess potent inhibitory activity to human cyp3a4, its dihydro analogue was inactive. this study revealed that the c1-c10b double bond is required for inhibition of this crucial metabolizing enzyme. compound 5 also demonstrated no inhibition of the related ... | 2010 | 21105682 |
| evolution of ligand specificity in vertebrate corticosteroid receptors. | corticosteroid receptors include mineralocorticoid (mr) and glucocorticoid (gr) receptors. teleost fishes have a single mr and duplicate grs that show variable sensitivities to mineralocorticoids and glucocorticoids. how these receptors compare functionally to tetrapod mr and gr, and the evolutionary significance of maintaining two grs, remains unclear. | 2011 | 21232159 |
| In vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L., Curcuma longa L. and Pancratium maritimum L. on Acanthamoeba castellanii cysts. | Acanthamoeba castellanii causes amoebic keratitis which is a painful sight-threatening disease of the eyes. Its eradication is difficult because the amoebas encyst making it highly resistant to anti-amoebic drugs, but several medicinal plants have proven to be more effective than the usual therapy. This study aimed to evaluate the in vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L. (peanut), Curcuma longa L. (turmeric), and Pancratium maritimum L. (sea daffodil) on A. castel ... | 2011 | 22146994 |
| poisonous plants in new zealand: a review of those that are most commonly enquired about to the national poisons centre. | new zealand has a number of plants, both native and introduced, contact with which can lead to poisoning. the new zealand national poisons centre (nznpc) frequently receives enquiries regarding exposures to poisonous plants. poisonous plants can cause harm following inadvertent ingestion, via skin contact, eye exposures or inhalation of sawdust or smoked plant matter. | 2012 | 23321887 |
| (2s,3r,4s,4ar)-2,3,4,7-tetra-hydroxy-3,4,4a,5-tetra-hydro-[1,3]dioxolo[4,5-j]phenanthridin-6(2h)-one hemihydrate. | the title natural compound, isolated from narcissus pseudonarcissus var. king alfred crystallizes as a hemihydrate, c14h13no7·0.5h2o, with four crystallographically independent dioxolophenanthridinone mol-ecules and two crystallographically independent solvent water mol-ecules in the asymmetric unit. all four crystallographically independent dioxolophenanthridinone mol-ecules are geometrically very similar and differ only in the orientations of the three hy-droxy groups at the terminal cyclo-hex ... | 2012 | 23476470 |
| on the mechanism of the lycopene isomerase/cyclase reaction in narcissus pseudonarcissus l. chromoplasts. | the lycopene cyclase reaction was investigated in narcissus pseudonarcissus l. membranes using the terminal desaturation product of the system, 7,9,9',7'-tetra-cis-lycopene (prolycopene) as substrate. the enzymatically formed bicyclic carotenes were trans configured, so the overall reaction consists of two individual processes, isomerization and cyclization. the two reactions were experimentally distinguished by use of the inhibitor [2-(p-chlorophenylthio)triethylammonium chloride. nadph, the es ... | 2007 | 1894603 |
| management of ditylenchus dipsaci in daffodils with foliar applications of oxamyl. | ditylenchus dipsaci (kühn) filipjev is a serious pest of commercial daffodil (narcissus sp.) in northern california. the control measures practiced by growers in recent years are postharvest treatment of planting bulbs for 3 hours in a 1% solution of formalin at 44 c combined with preplant soil fumigation with 1,3-dicbloropropene and (or) at-planting applications of phorate. in field trials, several combinations of rates (1.12, 2.24, or 4.48 kg a.i./ha in 189 liters of water/ha) and timings (one ... | 1991 | 19283189 |
| distribution of ditylenchus dipsaci in daffodil bulbs. | | 1986 | 19294232 |
| cloning and characterization of a norbelladine 4'-o-methyltransferase involved in the biosynthesis of the alzheimer's drug galanthamine in narcissus sp. aff. pseudonarcissus. | galanthamine is an amaryllidaceae alkaloid used to treat the symptoms of alzheimer's disease. this compound is primarily isolated from daffodil (narcissus spp.), snowdrop (galanthus spp.), and summer snowflake (leucojum aestivum). despite its importance as a medicine, no genes involved in the biosynthetic pathway of galanthamine have been identified. this absence of genetic information on biosynthetic pathways is a limiting factor in the development of synthetic biology platforms for many import ... | 2014 | 25061748 |
| in situ metabolic profiling of single cells by laser ablation electrospray ionization mass spectrometry. | depending on age, phase in the cell cycle, nutrition, and environmental factors, individual cells exhibit large metabolic diversity. to explore metabolic variations in cell populations, laser ablation electrospray ionization (laesi) mass spectrometry (ms) was used for the in situ analysis of individual cells at atmospheric pressure. single cell ablation was achieved by delivering mid-ir laser pulses through the etched tip of a geo(2)-based glass fiber. metabolic analysis was performed from singl ... | 2009 | 19824712 |
| asymmetrical mating patterns and the evolution of biased style-morph ratios in a tristylous daffodil. | non-random mating in plant populations can be influenced by numerous reproductive and demographic factors, including floral morphology and inter-plant distance. here, we investigate patterns of outcrossed mating through male function in narcissus triandrus, a tristylous, bee-pollinated wild daffodil from the iberian peninsula, to test pollen transfer models which predict that floral morphology promotes asymmetrical mating and biased morph ratios. unlike other tristylous species, n. triandrus has ... | 2008 | 18289396 |
| herkogamy and mating patterns in the self-compatible daffodil narcissus longispathus. | floral design in self-compatible plants can influence mating patterns. this study investigated narcissus longispathus, a self-compatible bee-pollinated species with wide variation in anther-stigma separation (herkogamy), to determine the relationship between variation in this floral trait and the relative amounts of cross- and self-fertilization. | 2005 | 15797899 |
| lectins with anti-hiv activity: a review. | lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-hiv activity. the anti-hiv plant lectins include artocarpus heterophyllus (jacalin) lectin, concanavalin a, galanthus nivalis (snowdrop) agglutinin-related lectins, musa acuminata (banana) lectin, myrianthus holstii lectin, narcissus pseudonarcissus lectin, and urtica diocia agglutinin. the anti-hiv algal ... | 2015 | 25569520 |
| [2-(14)c-methylerythritol 2,4-cyclodiphosphate incorporation into bacterial and plant isoprenoids]. | to work out a test system for studying the inhibition of isoprenoid biosynthesis through non-mevalonate pathway, the conversion of 2-(14)c-methylerythritol 2,4-cyclodiphosphate (14c-mec) into isoprenoids of three bacteria species and into plastids of 11 higher plant species was studied. the highest efficiency (up to 63%) of the process was observed with chromoplasts from narcissus (narcissus pseudonarcissus l.) and celandine (chelidonium majus l.). twenty five percent of added 14c-mec was recove ... | 2013 | 19845288 |
| a high-performance liquid chromatography method for the analysis of intermediates of the deoxyxylulose phosphate pathway. | a sensitive and versatile ion pair radio high-performance liquid chromatography (hplc) method for the investigation of the deoxyxylulose phosphate (dxp) pathway has been developed, allowing the simultaneous separation of phosphorylated, nonphosphorylated, and nucleotide moieties bearing intermediates. moreover, this method addresses the problem of separating the isomers isopentenyl diphosphate (idp) and dimethylallyl diphosphate (dmadp). because the majority of the intermediates of this isopreno ... | 2004 | 15556562 |
| studies on the nonmevalonate pathway of terpene biosynthesis. the role of 2c-methyl-d-erythritol 2,4-cyclodiphosphate in plants. | 2c-methyl-d-erythritol 2,4-cyclodiphosphate was recently shown to be formed from 2c-methyl-d-erythritol 4-phosphate by the consecutive action of ispd, ispe, and ispf proteins in the nonmevalonate pathway of terpenoid biosynthesis. to complement previous work with radiolabelled precursors, we have now demonstrated that [u-13c5]2c-methyl-d-erythritol 4-phosphate affords [u-13c5]2c-methyl-d-erythritol 2,4-cyclodiphosphate in isolated chromoplasts of capsicum annuum and narcissus pseudonarcissus. mo ... | 2001 | 11733027 |
| lipid-transfer proteins. | lipid-transfer proteins (ltps) are basic proteins found in abundance in higher plants. ltps play lots of roles in plants such as participation in cutin formation, embryogenesis, defense reactions against phytopathogens, symbiosis, and the adaptation of plants to various environmental conditions. in addition, ltps from field mustard and chinese daffodil exhibit antiproliferative activity against human cancer cells. ltps from chili pepper and coffee manifest inhibitory activity against fungi patho ... | 2012 | 23193591 |
| lectin-binding sites in epithelial cells of the mouse prostate gland. | the prostate is an exocrine gland in the male reproductive tract that secretes seminal fluids. to gain insight into the cytochemical properties of prostatic epithelial cells, the characteristics of glycoconjugates in mouse prostate sections were examined by lectin histochemistry and immunohistochemistry. characteristic staining patterns were observed, depending on the type of lectins present in the epithelia. luminal cells reacted specifically with mannose-binding lectins (galanthus nivalis lect ... | 2014 | 26004072 |
| a lectin microarray study of glycoantigens in neonatal porcine islet-like cell clusters. | besides α-gal expression, the differences of glycosylation and antigenicity between adult pig islets (apis) and neonatal porcine islet-like cell clusters (npccs) are altogether unclear. in this study, lectin microarray analyses of npccs were performed and the results compared with the corresponding values for wild-type apis and npccs from α-gal transferase knockout (galt-ko) pig. | 2013 | 23769020 |
| seed development and maturation in early spring-flowering galanthus nivalis and narcissus pseudonarcissus continues post-shedding with little evidence of maturation in planta. | seeds of the moist temperate woodland species galanthus nivalis and narcissus pseudonarcissus, dispersed during spring or early summer, germinated poorly in laboratory tests. seed development and maturation were studied to better understand the progression from developmental to germinable mode in order to improve seed collection and germination practices in these and similar species. | 2013 | 23478943 |
| insights into carbohydrate recognition by narcissus pseudonarcissus lectin: the crystal structure at 2 a resolution in complex with alpha1-3 mannobiose. | carbohydrate recognition by monocot mannose-binding lectins was studied via the crystal structure determination of daffodil (narcissus pseudonarcissus) lectin. the lectin was extracted from daffodil bulbs, and crystallised in the presence of alpha-1,3 mannobiose. molecular replacement methods were used to solve the structure using the partially refined model of hippeastrum hybrid agglutinin as a search model. the structure was refined at 2.0 a resolution to a final r -factor of 18.7 %, and rfree ... | 1999 | 10388566 |
| blot analysis with lectins for the evaluation of glycoproteins in cultured cells and tissues. | the carbohydrate-binding properties of plant and animal lectins have found many diverse applications in biomedical research that are comprehensively treated in the various chapters of this book. in our studies, lectins are often applied for the in situ detection of their respective binding sites in specific cells either grown in culture or present in various tissues (see chapter 3 ). in order to complement these in situ localization studies, blots are analyzed using lectins to identify glycosyla ... | 1998 | 21374457 |
| serum glycoprotein biomarker discovery and qualification pipeline reveals novel diagnostic biomarker candidates for esophageal adenocarcinoma. | we report an integrated pipeline for efficient serum glycoprotein biomarker candidate discovery and qualification that may be used to facilitate cancer diagnosis and management. the discovery phase used semi-automated lectin magnetic bead array (lemba)-coupled tandem mass spectrometry with a dedicated data-housing and analysis pipeline; glycoselector (http://glycoselector.di.uq.edu.au). the qualification phase used lectin magnetic bead array-multiple reaction monitoring-mass spectrometry incorpo ... | 2015 | 26404905 |