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isolation of two fractions with mevalonate kinase activity from pinus pinaster and agave americana. 197311947051
[purpuric contact dermatitis from agave americana].irritant papulovesicular dermatitis after cutaneous exposure to the sap of agave americana, which contains chemical irritants such as calcium oxalate and saponins, is well known, but purpuric dermatitis is rare in such cases.200717507849
green synthesis, characterisation and biological evaluation of agnps using agave americana, mentha spicata and mangifera indica aqueous leaves extract.the current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (agnps) of agave americana, mentha spicata and mangifera indica leaves and to screen them for biological activities. the ultraviolet-visible spectroscopic analysis revealed that λ-max for agnps range from 350-500 nm. all agnps possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in x-ray diffraction measurements an ...201627676375
prospecting for energy-rich renewable raw materials: agave leaf case study.plant biomass from different species is heterogeneous, and this diversity in composition can be mined to identify materials of value to fuel and chemical industries. agave produces high yields of energy-rich biomass, and the sugar-rich stem tissue has traditionally been used to make alcoholic beverages. here, the compositions of agave americana and agave tequilana leaves are determined, particularly in the context of bioethanol production. agave leaf cell wall polysaccharide content was characte ...201526305101
laboratory study on the mosquito larvicidal properties of leaf and seed extract of the plant agave americana.experiments on the leaf extract tested against three mosquito species led to 100% mortality of stage 4 anopheles, aedes and culex larvae at a concentration of 0.08% within 24-48 h, whereas 100% mortality of stage 1 larvae occurred at lower concentrations: 0.0032% for aedes aegypti, 0.016% for culex quinquefasciatus and 0.08% for anopheles stephensi, and was more rapid. at the end of 24 h, the highest dilution of the seed extract studied (1:200) produces a larval mortality of 100% for anopheles a ...19872882030
anti-inflammatory activity of different agave plants and the compound cantalasaponin-1.species of the agave genus, such as agave tequilana, agave angustifolia and agave americana are used in mexican traditional medicine to treat inflammation-associated conditions. these plants' leaves contain saponin compounds which show anti-inflammatory properties in different models. the goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema ...201323846754
intracuticular wax fixes and restricts strain in leaf and fruit cuticles.this paper investigates the effects of cuticular wax on the release of strain and on the tensile properties of enzymatically isolated cuticular membranes (cms) taken from leaves of agave (agave americana), bush lily (clivia miniata), holly (ilex aquifolium), and ivy (hedera helix) and from fruit of apple (malus × domestica), pear (pyrus communis), and tomato (lycopersicon esculentum). biaxial strain release was quantified as the decrease in cm disc area following wax extraction. stiffness, maxim ...201323750808
bacterial consortia constructed for the decomposition of agave biomass.research has shown that a greater variety of enzymes, as well as variety of microorganisms producing enzymes, can have an overall synergistic effect on the decomposition of lignocellulosic biomass for the production of value-added bio-products. here, 8 cellulase-degrading bacterial isolates were selected to develop co-, tri-, and tetra-cultures for the decomposition of lignocellulosic biomass. glucose and xylose equivalents released from imitation biomass media containing 0.5% (w/v) beechwood xy ...201724637707
introduction: contexts and concepts of adaptability and plasticity in 20th-century plant science.nowhere is the problem of understanding the complex linkages between organisms and their environments more apparent than in the science of plants. today, efforts by scientists to predict and manage the biological consequences of shifting global and regional climates depend on understanding how organisms respond morphologically, physiologically, and behaviorally to changes in their environments. investigating organismal "adaptability" (or "plasticity") is rarely straightforward, prompting controv ...201525641218
twenty-first century plant biology: impacts of the arabidopsis genome on plant biology and agriculture. 201020921172
in vitro cytotoxicity study of agave americana, strychnos nuxvomica and areca catechu extracts using mcf-7 cell line.research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. in present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as agave americana, strychnos nuxvomica and areca catechu using mcf-7 and vero cell line. various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. by ...201022247852
effect of agave americana and agave salmiana ripeness on saponin content from aguamiel (agave sap).steroidal saponins have shown beneficial health effects. agave spp. leaves and rhizomes are sources of these compounds, but their presence has not been reported in the aguamiel. aguamiel is the sweet edible sap from mature agave, and its quality is influenced by the plant ripening stage. the purpose of this research was to identify and quantitate saponins in aguamiel from agave americana and agave salmiana at two ripening stages. saponins and sapogenins were identified with hplc/esi-ms/tof and q ...201525811101
[negative air ions generated by plants upon pulsed electric field stimulation applied to soil].this paper investigated the capacity of plants (schlumbergera truncata, aloe vera var. chinensis, chlorophytum comosum, schlumbergera bridgesii, gymnocalycium mihanovichii var. friedrichii, aspidistra elatior, cymbidium kanran, echinocactus grusonii, agave americana var. marginata, asparagus setaceus) to generate negative air ions (nai) under pulsed electric field stimulation. the results showed that single plant generated low amounts of nai in natural condition. the capacity of c. comosum and g ...201526094455
agave as a model cam crop system for a warming and drying world.as climate change leads to drier and warmer conditions in semi-arid regions, growing resource-intensive c3 and c4 crops will become more challenging. such crops will be subjected to increased frequency and intensity of drought and heat stress. however, agaves, even more than pineapple (ananas comosus) and prickly pear (opuntia ficus-indica and related species), typify highly productive plants that will respond favorably to global warming, both in natural and cultivated settings. with nearly 200 ...201526442005
protective effect of agave americana linn. leaf extract in acetic acid-induced ulcerative colitis in rats.natural plants always provide core compounds for new drug development. in the present life and food style, inflammatory bowel disease has become common and needs a lead compound for its drug development.201726730148
effect of plant growth-promoting bacteria on the growth and fructan production of agave americana l.the effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in agave americana was assessed. the bacterial strains aco-34a, aco-40, and aco-140, isolated from the a. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. the three bacterial strains were evaluated via plant inoculation assays, and azospirillum brasilense cd served as a control strain. phylogenetic analysis based on the 16s rrna gene showe ...201627268113
gene stacking in transgenic plants--the challenge for 21st century plant biotechnology.one of the major technical hurdles impeding the advance of plant genetic engineering and biotechnology is the fact that the expression or manipulation of multiple genes in plants is still difficult to achieve. although a small proportion of commercial genetically modified (gm) crops present 'stacked' or 'pyramided' traits, only a handful of products have been developed by introducing three or more novel genes. on the research front, a variety of conventional and more novel methods have been empl ...200517173615
competitive sorption-desorption behavior of triazine herbicides with plant cuticular fractions.sorption interactions of plant cuticular matter with organic compounds are not yet fully understood. the objective of this study was to examine the competitive sorption-desorption interactions of the triazine herbicides (atrazine and ametryn) with cuticular fractions isolated from tomato fruits and leaves of agave americana. the 13c nmr data suggest a rubber-like nature for the cutin. this biopolymer exhibited reversible and noncompetitive sorption. enhanced desorption of atrazine was recorded i ...200617002450
an aminopeptidase from agave americana variegata--i: isolation, purification and physical and chemical characterization. 200025796
[contact dermatitis from agave americana].numerous plant species and their derivatives can cause skin reactions through a variety of mechanisms: irritative contact dermatitis, allergic contact dermatitis, contact urticaria and photodermatitis. we present a case of irritative contact dermatitis after exposure to the sap of agave americana. the skin symptoms in this case have only been described on rare occasions; although this condition usually presents with a papulovesicular rash, in this patient it appeared as purpuric lesions in the c ...200516476291
a large-scale screen for artificial selection in maize identifies candidate agronomic loci for domestication and crop improvement.maize (zea mays subsp mays) was domesticated from teosinte (z. mays subsp parviglumis) through a single domestication event in southern mexico between 6000 and 9000 years ago. this domestication event resulted in the original maize landrace varieties, which were spread throughout the americas by native americans and adapted to a wide range of environmental conditions. starting with landraces, 20th century plant breeders selected inbred lines of maize for use in hybrid maize production. both dome ...200516227451
constituents of agave americana and agave barbadensis.an investigation of agave americana and agave barbadensis resulted in the isolation of a new homoisoflavanoid, 7-hydroxy-3-(4-methoxybenzyl)-chroman (3), together with known compounds 7-hydroxy-3-(4-methoxybenzyl)-chroman-4-one (1), 5,7-dihydroxy-3-(4-methoxybenzyl)-chroman-4-one (2), cantalasaponin-1 (4), and 2-hydroxy-butanedioic acid-1-methyl ester (5).200515979249
adenosine 3':5'-cyclic monophosphate in higher plants: isolation and characterization of adenosine 3':5'-cyclic monophosphate from kalanchoe and agave.1.3':5'-cyclic amp was extensively purified from kalanchoe daigremontiana and agave americana by neutral alumina and anion- and cation-exchange column chromatography. inclusion of 3':5'-cyclic [8-3h]amp from the point of tissue extraction permitted calculation of yields. the purification procedure removed contaminating material that was shown to interfere with the 3':5'-cyclic amp estimation and characterization procedures. 2. the partially purified 3':5'-cyclic amp was quantified by means of a ...2003196595
four new steroid constituents from the waste residue of fibre separation from agave americana leaves.three new steroidal saponins, named agamenosides h-j (1-3), and a new cholestane steroid agavegenin d (4) were isolated from the waste residue of fibre separation from agave americana leaves, together with six known steroids. structures of the new compounds 1-4 were deduced to be (22s,23s,24r,25s)-24-[(beta-d-glucopyranosyl)oxy]-5alpha-spirostane-3beta,6alpha,23-triol 6-o-beta-d-glucopyranoside (1), (22s,23s,24r,25s)-5alpha-spirostane-3beta,23,24-triol 24-o-beta-d-glucopyranoside (2), (22s,23s,2 ...200415187383
botanical briefs: the century plant--agave americana l. 200314533828
[a new steroidal glycoside from fermented leaves of agave americana].to investigate the constituents of fermented leaves of agave americana, and discover new compounds.200212774633
leishmania donovani: assessment of leishmanicidal effects of herbal extracts obtained from plants in the visceral leishmaniasis endemic area of bihar, india.one obstacle faced in the effective control of visceral leishmaniasis (vl) is the limited number of available treatment options. furthermore, control efforts have been hindered further by the emergence of leishmania resistance to many of the available drugs. in this study, we investigated the anti-leishmanial properties of 30 medicinally important plants from the vl endemic area of bihar, india and compared them to two available anti-leishmanial drugs (sodium antimony gluconate and amphotericin ...201021070771
mexican plants and human fertility.synthetic steroids are obtainable cheaply and in abundance from sapogenins, substances originating from plants of the discorea family. some 40 years ago, russell marker, an american chemist, discovered this source, which grows abundantly in mexican jungles and is now exploited and cultivated commercially. today synthetic steroids prepared from extracts from a wide range of vegetable sources are used in the treatment of rheumatoid arthritis, allergies, inflammatory diseases, sterility, and vari ...197912309933
characterization and biosynthesis of non-degradable polymers in plant cuticles.the structure and monomeric composition of the highly aliphatic and non-saponifiable fraction of cutans isolated from the leaf cuticles of agave americana l. and clivia miniata reg. have been elucidated. spectroscopic fourier transform infrared and 13c-nuclear magnetic resonance, calorimetric and x-ray diffraction studies, together with biopolymer analysis after exhaustive ozonolysis, showed that the cutan fraction consists of an amorphous three-dimensional network linked by ether bonds containi ...199910333583
purpuric agave dermatitis.agave americana is a low growing, thick, long-leaved, subtropical plant used for medicinal, commercial, and ornamental purposes. the plant's sap contains calcium oxalate crystals, acrid oils, saponins, and other compounds. despite these known irritants, agave-induced irritant dermatitis has rarely been reported. previous case reports have noted a papulovesicular eruption consistent with an irritant contact dermatitis. we report a case of agave-induced purpura in an otherwise healthy patient. his ...199910025868
contact dermatitis with systemic symptoms from agave americana.twelve cases of contact dermatitis provoked by agave americana are described, 10 with systemic signs and symptoms and 8 with abnormal laboratory results.19989669116
acute irritant contact dermatitis from agave americana l. 19957493474
[dermatitis from contact with agave americana].various plants induce dermatitis in man. there have been only a few published cases of contact dermatitis caused by agave americana (aa). we report intentional exposure to aa in a soldier seeking sick leave, and review our previously reported cases. treatment with oral antihistamines and topical saline compresses resulted in subsidence of the systemic symptoms within 24 h and regression of cutaneous manifestations in 7-10 days. physicians should be alert to the possibility of self-inflicted cont ...200011062971
a new steroidal saponin from the leaves of agave americana.a new bisdesmosidic spirostanol saponin, along with three known saponins, were isolated from agave americana (agavaceae). the structure of the new saponin was elucidated as (25r)-3 beta,6 alpha-dihydroxy-5 alpha-spirostan-12-one 3,6-di-o-beta-d-glucopyranoside. among the isolated saponins, hecogenin tetraglycoside showed cytotoxic activity against hl-60 human promyelocytic leukemia cells with an ic50 value of 4.3 micrograms/ml.200010865469
screening of zulu medicinal plants for angiotensin converting enzyme (ace) inhibitors.twenty plants used by traditional healers in south africa for the treatment of high blood pressure were investigated for their anti-hypertensive properties, utilizing the angiotensin converting enzyme assay. a hit rate of 65% was achieved, with the highest inhibition (97%) obtained by adenopodia spicata leaves. a further seven plants exhibited an inhibition greater than 70% and five more over 50%. the leaves of the plants showed the greatest levels of inhibition. there was little difference in t ...199910624863
the molluscicidal properties of agave decepiens and agave americana (var. marginata). 19846736709
anti-inflammatory activity of aqueous extracts and steroidal sapogenins of agave americana.lyophilized aqueous extracts obtained from agave americana l (agavaceae) collected in the north of sardinia were characterized with regard to their steroidal sapogenin content. extracts of a. americana and genins isolated from them were evaluated for anti-inflammatory properties by testing their effects on carrageenin-induced edema. the effect of orally administered genins on gastric mucous membranes was also assessed. lyophilized extracts administered by the intraperitoneal route at doses equiv ...19979225599
[topography of the metabolic cycle of 4-aminobutyrate].this work describes, with some detail the intervention of 4-aminobutyrate as protagonist of a derivation of tricarboxylic cycle. its vicarial mission is emphasized in connection with its existence in microorganisms (escherichia coli and pseudomonas fluorescens), plants (helianthus tuberosus. lupinus albus and agave americana), neoplasic cells (ascitic tumor of ehrlich and hela cells) and animal tissues (adrenal medulla and brain.19826815735
isolation and characterization of an utero-active compound from agave americana.crude extracts of agave americana contain two utero-active compounds. one of these, tentatively named "fraction b", has been purified to chromatographic homogeneity. its pharmacological actions are similar to those of acetylcholine. however its chromatographic and electrophoretic mobilities are different. some chemical properties of fraction b are compatible with the structure of an acyl derivative of choline different from acetylcholine.19892640501
peroxisomal and mitochondrial citrate synthase in cam plants.citrate synthase wa studied for the first time in peroxisomes and mitochondria of crassulacean acid metabolism plants. cellular organelles were isolated from agave americana leaves by sucrose density gradient centrifugation and characterized by the use of catalase and cytochrome oxidase as marker enzymes, respectively. 48,000 x g centrifugation caused the breakdown of the cellular organelles. the presence of a glyoxylate cycle enzyme (citrate synthase) and a glycollate pathway enzyme (catalase) ...19817339746
an aminopeptidase from agave americana variegata--ii: aspects of enzyme kinetics, specificity, inhibition and mechanism of action. 197825797
influence of several feeds on bacteria in sheep and goat rumen liquor in vitro.bacteriological studies were made with in vitro sheep and goat ruminal fluids supplemented with several feeds (alfalfa hay, wheat straw, agave americana, opuntia ficus indica and atriplex nummularia) during anaerobic incubation at 38-39 degrees c. drastic changes in the bacterial population of sheep ruminal fluids occurred in the presence of different feeds, particularly with addition of feeds of low nutritional quality (wheat straw, a. americana and o. ficus indica). however, the bacterial popu ...19902370837
[agave americana causing irritant contact dermatitis with a purpuric component]. 201121315871
a third type of raphide crystal in the plant kingdom: six-sided raphides with laminated sheaths in agave americana l.raphides in leaves of agave americana l. have six-sided cross sections. each crystal tapers off to a point at both ends. it is enveloped in a 100 nm thick sheath which, in cross section, shows lamellae with periods of 6-9 nm. no polysaccharides could be detected in the sheaths with the thiéry reaction. dissolution of a raphide in acid occurs slowly from both ends leaving the crystal sheath visible in the light microscope. the raphide cell walls contain a layer that in glutaraldehyde-fixed tissue ...197624424644
[the saponins in the leaves of agave americana l]. 19751161792
irritant contact dermatitis from agave americana l. incorrect use of sap as "hair restorer". 19734716728
effect of ambient carbon dioxide concentration on the rate of transpiration of agave americana in the dark. 197016058736
the steroid sapogenin constituents of agave americana, a. variegata and yucca gloriosa. 19705484889
[application of thin-layer chromatography in the study of natural products. iv. identification of steroidal sapogenins from agave americana l]. 196414147596
the chemistry of plant cuticles: a study of cutin from agave americana l. 195613353926
a stiving for scientific respectability: some american botanics and the nineteenth-century plant materia medica. 200913304508
new technologies for 21st century plant science.plants are one of the most fascinating and important groups of organisms living on earth. they serve as the conduit of energy into the biosphere, provide food, and shape our environment. if we want to make headway in understanding how these essential organisms function and build the foundation for a more sustainable future, then we need to apply the most advanced technologies available to the study of plant life. in 2009, a committee of the national academy highlighted the "understanding of plan ...201222366161
an improved method for thin layer chromatographic analysis of saponins.analysis of saponins by thin layer chromatography (tlc) is reported. the solvent system was n-butanol:water:acetic acid (84:14:7). detection of saponins on the tlc plates after development and air-drying was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood on the plate surface. saponins appeared as white spots against a pink background. the protocol provided specific detection of saponins in the saponins enriched extracts from aesculusindica (wall ...201226434348
allelic variation at the rht8 locus in a 19th century wheat collection.wheat breeding during the 20th century has put large efforts into reducing straw length and increasing harvest index. in the 1920s an allele of rht8 with dwarfing effects, found in the japanese cultivar "akakomugi," was bred into european cultivars and subsequently spread over the world. rht8 has not been cloned, but the microsatellite marker wms261 has been shown to be closely linked to it and is commonly used for genotyping rht8. the "akakomugi" allele is strongly associated with wms261-192bp. ...201222654600
analysis of gene sequences indicates that quantity not quality of chloroplast small hsps improves thermotolerance in c4 and cam plants.chloroplast-localized small heat-shock proteins (cp-shsp) protect photosystem ii and thylakoid membranes during heat and other stresses, and cp-shsp production levels are related to plant thermotolerance. however, to date, a paucity of cp-shsp sequences from c4 or cam species, or from other extremely heat-tolerant species, has precluded an examination to determine if cp-shsp genes or proteins might differ among plants with photosynthetic pathways or between heat-sensitive and heat-tolerant speci ...201222797908
pretreatment of agave americana stalk for enzymatic saccharification.agave americana is one of commonly grown agave species but currently less valuable because its large flower stalk cannot be used for producing alcoholic beverage. in the present study, the stalk was pretreated with dilute acid (da), sulfite (sporl), and sodium hydroxide (naoh) to preliminarily assess its potential as feedstock for bioethanol production. the changes of cell wall components during the pretreatments, enzymatic digestibility of the pretreated stalks, and the adsorption of cellulases ...201223122484
adverse effects of herbal medicines: an overview of systematic reviews.this overview of systematic reviews (srs) aims to evaluate critically the evidence regarding the adverse effects of herbal medicines (hms). five electronic databases were searched to identify all relevant srs, with 50 srs of 50 different hms meeting our inclusion criteria. most had only minor weaknesses in methods. serious adverse effects were noted only for four hms: herbae pulvis standardisatus, larrea tridentate, piper methysticum and cassia senna. the most severe adverse effects were liver o ...201323472485
phytodermatitis caused by agave americana. 201121196071
structural analysis of fructans from agave americana grown in south africa for spirit production.fructans isolated from agave americana grown in south africa are currently used for spirit production. structural studies on water-soluble fructans were performed to facilitate the development of other applications including its use as a prebiotic. acid hydrolysis followed by hpaec-pad analysis confirmed that the fructan was composed of glucose and fructose, and size analysis by hpaec-pad and size exclusion chromatography indicated that the saccharides have a dp range from 6 to 50. an average dp ...200919348427
isolation, characterization, and localization of agasgnh cdna: a new sgnh-motif plant hydrolase specific to agave americana l. leaf epidermis.gdsl and sgnh hydrolases are lipases involved in a wide range of functions, behaving in many cases as bifunctional enzymes. in this work, the isolation and characterization of agasgnh, a cdna encoding a member of the sgnh-hydrolase superfamily from young leaf epidermis of the monocot agave americana l., is reported. the protein possesses a typical signal peptide at its n-terminus that allows its secretion to the epidermis cell wall, as verified by immunolocalization experiments. in addition, the ...200717609535
method and metaphysics in clements's and gleason's ecological explanations.to generate explanatory theory, ecologists must wrestle with how to represent the extremely many, diverse causes behind phenomena in their domain. early twentieth-century plant ecologists frederic e. clements and henry a. gleason provide a textbook example of different approaches to explaining vegetation, with clements allegedly committed, despite abundant exceptions, to a law of vegetation, and gleason denying the law in favor of less organized phenomena. however, examining clements's approach ...200717324810
21st century plant biology: viva la revolucion? 200717293564
agave (agave americana): an evidence-based systematic review by the natural standard research collaboration.an evidence-based systematic review including written and statistical analysis of scientific literature, expert opinion, folkloric precedent, history, pharmacology, kinetics/dynamics, interactions, adverse effects, toxicology, and dosing.200617182489
isolation and characterization of a cdna encoding a membrane bound acyl-coa binding protein from agave americana l. epidermis.a cdna encoding an acyl-coa binding protein (acbp) homologue has been cloned from a cdna library made from mrna isolated from epidermis of young leaves of agave americana l. the derived amino acid sequence reveals a protein corresponding to the membrane-associated form of acbps only previously described in arabidopsis and rice. northern blot analysis showed that the a. americana acbp gene is mainly expressed in the epidermis of mature zone of the leaves. the epidermis of a. americana leaves have ...200616531053
solid-state nmr characterization of pyrene-cuticular matter interactions.one- and two-dimensional nuclear magnetic resonance (nmr) experiments were performed on agave americana cutan and tomato cutin to examine the interactions between a hydrophobic pollutant, pyrene, and cuticular material. variable-temperature nmr experiments show that cutan, an acid- and base-resistant cuticular biopolymer, undergoes the characteristic melting behavior of "polyethylene-like" crystallites, while the tomato cutin does not. the melting point of a. americana cutan was found to be appr ...200415382866
three new hecogenin glycosides from fermented leaves of agave americana.eight steroidal compounds, including three new hecogenin glycosides, agamenosides d-f, were isolated from the fermented leaves of agave americana. the structures of the new steroidal saponins were elucidated by spectroscopic data and chemical methods. the activity of the isolated compounds on deformations of mycelia germinated from conidia of pyricularia oaryzae p-2b was evaluated.200312765193
purpuric irritant contact dermatitis induced by agave americana.the sap of agave americana, a popular ornamental plant, may cause irritant contact dermatitis. this rare eruption is typically vesiculopapular; however, a new purpuric variant with evidence of leukocytoclastic vasculitis has recently been reported. we report an additional case of a purpuric eruption associated with severe constitutional symptoms further supporting a possible vasculitic component. both cases resulted from direct exposure to sap propelled by a chainsaw. we speculate that oxalic ac ...200011109152
caffeic acid and glycerol are constituents of the suberin layers in green cotton fibres.the fibres of the green-lint mutant (lg) of cotton (gossypium hirsutum l.) are suberized and contain a large proportion of wax. the unidentified components of the wax were separated into a colourless fluorescent fraction and a yellow pigmented fraction. using ultraviolet spectroscopy and nuclear-magneticresonance ((1)h-nmr) spectroscopy, esterified trans-caffeic acid was identified as the only phenolic component in the colourless fraction. this fraction was further purified and was shown to cont ...199324178505
[effect of several extracts derived from plants on the survival of larvae of aedes fluviatilis (lutz) (diptera: culicidae) in the laboratory].the larvicidal properties of 34 plant extracts were tested against aedes fluviatilis (lutz) (diptera: culicidae) larvae, at 100, 10 and 1 ppm concentrations; 26.6% of the extracts enhanced larval mortality (alpha = 0.05) at 100 ppm (anacardium occidentale, agave americana, allium sativum, coriandrum sativum, nerium oleander, spatodea campanulata, tibouchina scrobiculata and vernonia salzmanni). anacardic acid (a. occidentale) was effective at 10 ppm and a. sativum (crude extract) at 1 ppm.20063249563
phytobezoar from the stem ("quiote") of the cactus agave americana: report of case.agave americana is a cactus growing abundantly in mexico. its cooked stem ("quiote") yields by mastication a sweet juice which is swallowed, while the fibers ("bagazo") are spit out. that is the way mexicans are taught to chew quiote since their early childhood, and it accounts for the rarity of bezoars from this origin. one of such cases is reported herein.19854050757
properties of peroxisomal and mitochondrial citrate synthase from agave americana.adenine nucleotides were tested as effectors of peroxisomal and mitochondrial citrate synthase from agave americana leaves in the presence of different concentrations of acetyl-coa and oxalacetate substrates. atp inhibited both enzyme activities but with a different inhibition profile. 1.0-7.5 mm adp did not inhibit the peroxisomal citrate synthase in the presence of high substrate concentrations, while the mitochondrial enzyme was strongly inhibited by 1.0 mm adp in the same conditions. likewis ...19827156452
[colorimetric method for the determination of hecogenin in agave americana l. residue]. 19827180493
composition and ultrastructure of the suberized cell wall of isolated crystal idioblasts from agave americana l. leaves.styloid-calcium-oxalate-crystal-containing idioblasts possess an interior cell-wall layer which has a lamellar ultrastructure. idioblasts were isolated by centrifugation of an agave americana leaf homogenate through 2m sucrose. the aliphatic monomers of the polymeric material from an idioblast fraction were primarily ω-hydroxy acids (32%) and dicarboxylic acids (35%), with c18:1 dicarboxylic acid being the most dominant monomer (25%). nitrobenzene oxidation of the idioblasts yielded syringaldehy ...198224271671
isolation and partial characterization of a protease from agave americana variegata.a new protease was isolated from an extract of leaves of agave americana variegata. the protease (ec 3.4.-) was purified 565-fold with a yield of 39.5%. the 43.8 mg enzyme had a specific activity of 0.44 units/mg. according to electrophoretic, ultracentrifugal and other physical characterizations the enzyme was homogeneous. the enzyme had a mr of 57000, a s20,w-value of 4.37 s, a d20, w-value of 6.8-7.0 - 10(-7) cm2sec-1, a stokes radius of 3.18 nm, a partial specific volume of 0.735 cm3g-1, a f ...19765146
ultrastructure of the suberized styloid crystal cells in agave leaves.styloid calcium oxalate crystal idioblasts of agave americana l. are suberized. where the crystals do not touch the cell wall directly they are enclosed in a suberinic sheath which is connected with the suberinic wall layer. no polysaccharides are laid down as a tertiary wall layer, nor could any polysaccharides be found in the crystal sheath. these results contradict those of arnott (1973) but agree fully with those of rothert and zalenski (1899).197624430692
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