Publications

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isolation and identification of intestinal steroid-desulfating bacteria from rats and humans.we isolated 12 strictly anaerobic steroid-3-sulfate-desulfating strains from the intestinal floras of rats and humans. two strains (s1 and s2) of the same atypical clostridium species and an atypical lactobacillus strain (termed r9) were obtained from rats. the human isolates were identified as eubacterium cylindroides (two strains, h1 and h2), peptococcus niger (two strains, h4 and h89), and clostridium clostridiiforme. we also isolated, from different human fecal samples, four strains of pheno ...19883178214
extensive set of 16s rrna-based probes for detection of bacteria in human feces.for the detection of six groups of anaerobic bacteria in human feces, we designed seven new 16s rrna-based oligonucleotide probes. this set of probes extends the current set of probes and gives more data on the composition of the human gut flora. probes were designed for phascolarctobacterium and relatives (phasco741), veillonella (veil223), eubacterium hallii and relatives (ehal1469), lachnospira and relatives (lach571), and eubacterium cylindroides and relatives (ecyl387), and two probes were ...200212039758
effects of alternative dietary substrates on competition between human colonic bacteria in an anaerobic fermentor system.duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. a panel of group-specific fluorescent in situ hybridization probes targeting 16s rrna sequences revealed that the fermentors supported growth of a greater proportion of bacteroides and a lower proportion of gram-positive anaerobes related to faecalibacterium prausnitzii, ruminococcus flavefaciens-ruminococcus bromii, eubacterium rectale-cl ...200312571040
studies on the effect of system retention time on bacterial populations colonizing a three-stage continuous culture model of the human large gut using fish techniques.fluorescence in situ hybridization was used to quantitate bacteria growing in a three-stage continuous culture system inoculated with human faeces, operated at two system retention times (60 and 20 h). twenty-three different 16s rrna gene oligonucleotide probes of varying specificities were used to detect bacteria. organisms belonging to genera bacteroides and bifidobacterium, together with the eubacterium rectale/clostridium coccoides group, the atopobium, faecalibacterium prausnitzii and eubac ...200616420637
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