| immunochemical characterization of juvenile hormone esterase from different species of lepidoptera. | cross-immunoreactivity of juvenile hormone esterase (jhe) from different species was tested using anti-jhe (trichoplusia ni) (noctuidae) polyclonal antibody. partial cross-reactivity was observed between jhe from hyphantria cunea, isia isabella (arctuidae) and spodoptera exigua (noctuidae) in immunoblot analysis. soluble antigen-antibody complex formation was observed between anti-jhe (t. ni) and antigen(s) from heliothis virescens (noctuidae) during immunotitration of antigen(s). using an elisa ... | 1987 | 3579958 |
| microsporida of the fall webworn, hyphantria cunea. i. identification, distribution, and comparison of nosema sp. with similar nosema spp. from other lepidoptera. | | 1974 | 4212168 |
| abnormal cell proliferation in the epidermis of the fall webworm, hyphantria cunea, induced by the infection of a nuclear-polyhedrosis virus. | | 1968 | 5716587 |
| bacillus thuringiensis serovar higo (flagellar serotype 44), a new serogroup with a larvicidal activity preferential for the anopheline mosquito. | eight strains of bacillus thuringiensis, isolated in japan, formed spherical parasporal inclusions and exhibited low to moderate larvicidal activities for two mosquito species, anopheles stephensi and culex pipiens molestus, but not for another dipteran, telmatoscopus albipunctatus, or two lepidopterans, bombyx mori and hyphantria cunea. the anopheline toxicity (lc50 = 6.3 micrograms ml-1) was > 10 times greater than the activity on the culex mosquito. these strains were assigned to a previously ... | 1995 | 7576527 |
| a trypsin inhibitor from snail medic seeds active against pest proteases. | a protein trypsin inhibitor from seeds of snail medic (medicago scutellata), msti, has been purified by ion-exchange chromatography, gel-filtration chromatography and reverse-phase hplc. the protein inhibits the catalytic activity of bovine beta-trypsin, with an apparent kd of 1.8 x 10(-9), but exhibits no activity towards bovine alpha-chymotrypsin. moreover, msti inhibits the trypsin-like proteinase activity present in larvae of the crop pests adoxophyes orana, hyphantria cunea, lobesia botrana ... | 1997 | 9014368 |
| protein purification and nucleotide sequence of a lysozyme from the bacteria-induced larvae of the fall webworm, hyphantria cunea. | a protein with lytic activity against micrococcus luteus was purified from the hemolymph of the fall webworm, hyphantria cunea, larvae challenged with live e. coli. a bacteriolytic protein of about 14,000 daltons in mass was purified by cation exchange chromatography and reverse-phased hplc. the optimum ph and optimum temperature range for activity were around ph 6.2 and 50 degrees c, respectively, in a 100 mm phosphate buffer. the amino-terminal amino acid sequence of this protein was determine ... | 1997 | 9177137 |
| protein purification and cdna cloning of a cecropin-like peptide from the larvae of fall webworm (hyphantria cunea). | a proteinous antimicrobial substance was purified from the bacteria-challenged larvae of the fall webworm, hyphantria cunea. it is a cecropin-like antibacterial peptide which exhibits antibacterial activity against gram-negative and gram-positive bacteria, and known as hyphantria cecropin a. the cdna clones corresponding to this peptide were isolated from a cdna library constructed from the bacteria-challenged larvae and obtained complete nucleotide sequences. in addition to the hyphantria cecro ... | 1997 | 9443371 |
| isolation and characterization of immune-related genes from the fall webworm, hyphantria cunea, using pcr-based differential display and subtractive cloning. | following injection of bacteria into the hemocoel of the fall webworm, hyphantria cunea, several inducible genes were identified and characterized using pcr-based differential display (dd-pcr) and subtractive cloning. ten immune-related cdna clones (hdd1, hdd2, hdd3, hdd11, hdd13, hdd15, hdd17, hdd23, hs106, hs302) were isolated and characterized. the deduced amino acid sequence of hdd2 was shown to be a member of the copper, zinc superoxide dismutase (cu-zn sod) family. the h. cunea cu-zn sod i ... | 1998 | 9818384 |
| two carbohydrate recognition domains of hyphantria cunea lectin bind to bacterial lipopolysaccharides through o-specific chain. | we previously identified a novel lectin cdna from the fall webworm [shin et al. (1998) insect biochem. mol. biol. 28, 827-837], which encodes two carbohydrate recognition domains (crd-n and crd-c) and is up-regulated following bacterial challenge. the lipopolysaccharide (lps) binding activities of the recombinant crd-n and crd-c (rcrd-n and rcrd-c) were investigated by enzyme-linked immunosorbent assay. the lps binding of rcrd-n and rcrd-c was ph-dependent: at ph below 6.0, they show a higher bi ... | 2000 | 10664459 |
| immunological detection of serpin in the fall webworm, hyphantria cunea and its inhibitory activity on the prophenoloxidase system. | we previously identified a serine type protease inhibitor (serpin) cdna, using pcr-based differential display, in the fall webworm which was up-regulated following a bacterial challenge (shin et al., 1998). the serpin cdna was inserted into an expression vector and the serpin protein was expressed in escherichia coli. in order to investigate the action of serpin in vivo, we examined the concentration of serpin protein in the larvae of hyphantria cunea by western blot analysis using a polyclonal ... | 2000 | 10850660 |
| cloning and functional expression of a chitinase cdna from the common cutworm, spodoptera litura, using a recombinant baculovirus lacking the virus-encoded chitinase gene. | a chitinase cdna named slchi was cloned from the epidermis of the common cutworm, spodoptera litura, and the enzymatic properties of its recombinant proteins were characterized. the slchi cdna encodes 552 amino-acid residues (aa) including a 19 aa putative signal peptide, with the calculated molecular mass of the putative mature protein 60,152 da. a major transcript of slchi about 2.8 kb was detected in the epidermis only during molting in the last instar larvae, suggesting its involvement in th ... | 2001 | 11267891 |
| inactivation analysis of hcnpv cysteine protease gene and chitinase gene. | the fragment of hyphantria cunea nuclear polyhedrosis virus (hcnpv), which contains cysteine protease gene(cp) and chitinase gene (chia),was inserted into plasmid pcrii to construct transfer vector phccvdel. recombinant transfer vector phccvpolh was constructed by inserting the fragment of hcnpv polyhedrin gene (polh) into the ecori site of the phccvdel. by contransfection of the phccvpolh and hcnpv-ptth(+) dna, which contained bombyx mori prothoracicotropic hormone gene (ptth) into spim cells, ... | 2000 | 12098800 |
| nucleotide sequence analysis of the hcnpv cysteine protease gene. | the cysteine protease (cp) gene of hyphantria cunea nuclear polyhedrosis virus (hcnpv) has been located at the hind iii--3.5 kb fragment. the nucleotide sequence has been determined. the results indicated that its open reading frame of 975 nt encoded the protein of 324 amino acids. the baculovirus late gene promoter taag motif was identified at -24 nt upstream from the start codon atg; the typical poly (a) signal sequence aataaa was detected at 33 nt downstream from the stop codon taa. the hcnpv ... | 1998 | 12174267 |
| [analysis and expression of hyphantria cunea nuclear polyhedrosis virus sod gene]. | the sequencing results indicated that hyphantria cunea nuclear polyhedrosis virus (hcnpv) sod gene open reading frame of 456 nt encoding protein of 151 amino acid, was identified to that of bombyx mori nuclear polyhedrosis virus (bmnpv), and exhibited 97.2% homology at nucletde level to that of autographa californica nuclear polyhedrosis virus (acnpv), three amino acid residues difference in amino acid level with acnpv sod. the essential amino acid residues for the construction and active could ... | 2001 | 12549022 |
| induction of apoptosis in an insect cell line, iplb-ld652y, infected with nucleopolyhedroviruses. | ld652y cells derived from the gypsy moth, lymantria dispar, were infected with seven different nucleopolyhedroviruses (npvs) including those from autographa californica, bombyx mori (bmnpv), hyphantria cunea (hycunpv), spodoptera exigua (semnpv), l. dispar, orgyia pseudotsugata (opmnpv) and spodoptera litura (spltmnpv). the results showed that ld652y cells infected with bmnpv, hycunpv, semnpv, opmnpv and spltmnpv underwent apoptosis, displaying apoptotic bodies, characteristic dna fragmentation ... | 2003 | 12604823 |
| identification and characterization of hyphantria cunea nucleopolyhedrovirus homologous repeated regions. | a total of six homologous regions (hycu-hrs1-6) were identified in the genome of hyphantria cunea nucleopolyhedrovirus (hycunpv). these hycu-hrs were localized in non-coding regions interspersed throughout the hycunpv genome and showed structural homology to several other baculovirus hrs. sequence analyses indicated that hycu-hrs were composed of 65-69-bp direct repeats, each of which contained a 29-31-bp imperfect palindrome embedded within non-palindromes tandemly arranged. hycu-hrs consisted ... | 2002 | 12881639 |
| cdna cloning and expression of bacteria-induced hdd11 gene from eri-silkworm, samia cynthia ricini. | a cdna clone encoding hdd11 protein, a bacteria-induced protein of unknown function, was isolated from fat body of immunized samia cynthia ricini larvae based on suppression subtractive hybridization. the cdna encodes a 167 amino acid residue open reading frame with an 18 residue predicted signal peptide. the deduced amino acid sequence showed 54% and 55% identity with hdd11 proteins from hyphantria cunea and manduca sexta, respectively. expression of the gene was undetectable in naive larvae wh ... | 2003 | 15012905 |
| identification and functional analysis of hyphantria cunea nucleopolyhedrovirus iap genes. | hyphantria cunea nucleopolyhedrovirus (hycunpv) infection protected spim cells from actinomycin d (actd)-induced apoptosis as early as 4 h postinfection. analysis by southern hybridization revealed that the hycunpv genome possessed three members of inhibitor of apoptosis genes (iaps) that were designated as hycu-iap1, hycu-iap2, and hycu-iap3 because of their amino acid sequence homology with iaps identified in other baculoviruses. functional analysis of hycu-iaps by transient expression assay i ... | 2004 | 15051395 |
| hyphantria cunea ferritin heavy chain homologue: cdna sequence and mrna expression. | we have sequenced a cdna clone encoding a 26-kda ferritin subunit, which was heavy chain homologue (hch), in fall webworm, hyphantria cunea. the hch cdna was obtained from the screening of a cdna library using a pcr product. h. cunea ferritin is composed of 221 amino acid residues and their calculated mass is 26,160 da. the protein contains the conserved motifs for the ferroxidase center typical for heavy chains of vertebrate ferritin. the iron-responsive element sequence with a predicted stem-l ... | 2004 | 15101063 |
| immune activation of apolipophorin-iii and its distribution in hemocyte from hyphantria cunea. | apolipophorin-iii (apolp-iii) is a hemolymph protein whose function is to facilitate lipid transport in an aqueous medium. recently, apolp-iii in galleria mellonella larvae was shown to play an unexpected role in insect immune activation. we identified the cdna sequence of hyphantria cunea apolp-iii by oligonucleotide-primed amplification, and 5'- and 3'-race pcr. since h. cunea has an unusually low level of apolp-iii in the hemolymph, a recombinant apolp-iii was overexpressed using a baculoviru ... | 2004 | 15475296 |
| host range factor 1 from lymantria dispar nucleopolyhedrovirus (npv) is an essential viral factor required for productive infection of npvs in iplb-ld652y cells derived from l. dispar. | host range factor 1 (hrf-1) of lymantria dispar multinucleocapsid nucleopolyhedrovirus promotes autographa californica mnpv replication in nonpermissive ld652y cells derived from l. dispar. here we demonstrate that restricted hyphantria cunea npv replication in ld652y cells was not due to apoptosis but was likely due to global protein synthesis arrest that could be restored by hrf-1. our data also showed that hrf-1 promoted the production of progeny virions for two other baculoviruses, bombyx mo ... | 2004 | 15507661 |
| accumulation of 23 kda lipocalin during brain development and injury in hyphantria cunea. | the cdna corresponding to a novel lipocalin was identified from the fall webworm, hyphantria cunea. this lipocalin cdna encodes a 194 residue protein with a calculated molecular mass of 23 kda. sequence analyses revealed that the 23 kda lipocalin cdna is most similar to drosophila lazarillo, human apolipoprotein d, and bombyrin. northern blot analyses showed that 23 kda lipocalin transcript is expressed in the whole body only in 4- and 6-day-old pupae. by western blot analysis it was confirmed t ... | 2005 | 16102419 |
| gene organization and complete sequence of the hyphantria cunea nucleopolyhedrovirus genome. | the whole-genome sequence of the hyphantria cunea nucleopolyhedrovirus (hycunpv) was analysed. the entire nucleotide sequence of the hycunpv genome was 132 959 bp long, with a g+c content of 45.1 mol%. a total of 148 open reading frames (orfs) consisting of more than 50 aa were encoded by the genome. hycunpv shares more than 122 orfs with other lepidopteran group i npvs, including autographa californica mnpv, bombyx mori npv, choristoneura fumiferana mnpv (cfmnpv), choristoneura fumiferana defec ... | 2006 | 16894193 |
| expression and characterization of a sigma-class glutathione s-transferase of the fall webworm, hyphantria cunea. | a cdna encoding glutathione s-transferase (gst) of the fall webworm, hyphantria cunea, was cloned by reverse transcriptase-polymerase chain reaction. the resulting clone (hcgst) was sequenced and deduced for amino acid sequence, which revealed 87, 59, and 42% identities to sigma-class gsts from bombyx mori, manduca sexta, and blattella germanica respectively. a recombinant hcgst protein (rhcgst) was functionally overexpressed in escherichia coli cells in a soluble form and purified to homogeneit ... | 2007 | 17284839 |
| comparative analysis of two attacin genes from hyphantria cunea. | a full-length clone corresponding to attacin was isolated from a cdna library made from fat body of immunized hyphantria cunea larvae. this newly isolated attacin b shows characteristics different from those previously reported for attacin a. the two attacin cdnas encode precursor proteins of 233 and 248 amino acid residues, respectively. the two attacins show 45.9% identity at the amino acid level, and 35.2% identity at the nucleotide level. attacins a and b of h. cunea show significant identit ... | 2008 | 18682300 |
| apolipophorin iii from hyphantria cunea shows different anti-oxidant ability against ldl oxidation in the lipid-free and lipid-bound state. | insect apolipophorin iii (apolp-iii) and human apolipoprotein a-i (apoa-i) are major protein constituents of the lipoprotein system that share various properties. in order to compare the anti-oxidant ability of apolp-iii and apoa-i in the lipid-free and lipid-bound state, both proteins were purified and synthesized individually as a palmitoyloleoyl phosphatidylcholine (popc)-reconstituted high-density lipoprotein (rhdl) using the same molar ratio. in the lipid-bound state, apolp-iii and apoa-i s ... | 2008 | 18822383 |
| biochemical analysis of hyphantria cunea npv attachment to spodoptera frugiperda 21 cells. | binding characteristics of hyphantria cunea nuclear polyhedrosis virus (hcnpv) to spodoptera frugiperda 21 (sf21) cells was determined. the cells displayed an affinity of 0.9 x 10(10) m(-1) with about 8900 binding sites per cell. the biochemical nature of hcnpv-binding sites on the cell surface was also partially elucidated. there were 45 to 49% reductions in hcnpv binding following the pretreatment of cells with three proteases, suggesting the involvement of a cellular protein component in viru ... | 1999 | 19003136 |
| quantitative resistance traits and suitability of woody plant species for a polyphagous scarab, popillia japonica newman. | the japanese beetle, popillia japonica newman, has an unusually broad host range among deciduous woody plants, yet it feeds only sparingly, or not at all, on certain species in the field. we evaluated beetles' preference, survival over time and fecundity on eight woody plant species historically rated as susceptible or resistant and, after verifying those ratings, tested whether resistance is correlated with so-called quantitative defense traits including leaf toughness, low nutrient content (wa ... | 2008 | 19161699 |
| hycu-hr6, a large homologous region of the hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems. | we previously identified a large homologous region (hr), hycu-hr6, in the genome of the hyphantria cunea nucleopolyhedrovirus (hycunpv) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. in this study, we examined the enhancement activity of hycu-hr6 against two promoters from constitutive baculovirus immediate early genes, the hycunpv ie1 (hycu-ie1) and the orgyia pseudotsugata multicapsid (m) npv ie2 (op-ie2), and against a promoter from the inducib ... | 2009 | 19809870 |
| identification of a hyphantria cunea nucleopolyhedrovirus (npv) gene that is involved in global protein synthesis shutdown and restricted bombyx mori npv multiplication in a b. mori cell line. | we previously demonstrated that bombyx mori nucleopolyhedrovirus (bmnpv) multiplication is restricted in permissive bmn-4 cells upon coinfection with hyphantria cunea npv (hycunpv). here, we show that hycunpv-encoded hycu-ep32 gene is responsible for the restricted bmnpv multiplication in hycunpv-coinfected bmn-4 cells. the only homologue for hycu-ep32 is in orgyia pseudotsugata npv. hycu-ep32 could encode a polypeptide of 312 amino acids, and it contains no characteristic domains or motifs to s ... | 2010 | 20034650 |
| comparative transient expression assay analysis of hycu-hr6- and ie1-dependent regulation of baculovirus gp64 early promoters in three insect cell lines. | we previously demonstrated that the hyphantria cunea multicapsid nucleopolyhedrovirus (hycumnpv) gp64 gene (hycu-gp64) is uniquely localized on the viral genome with a large homologous region of 1582bp, hycu-hr6, immediately upstream of the hycu-gp64 gene. in the present study, we compared the regulation of gp64 early promoters from hycumnpv, autographa californica multicapsid npv (acmnpv) and bombyx mori npv (bmnpv) by cis-acting hycu-hr6 and trans-acting ie1s in three cell lines (spodoptera fr ... | 2011 | 20837073 |
| baculovirus iap1 induces caspase-dependent apoptosis in insect cells. | baculoviruses encode inhibitors of apoptosis (iaps), which are classified into five groups, iap1, iap2, iap3, iap4 and iap5, based on their sequence homology. most of the baculovirus iaps with anti-apoptotic function belong to the iap3 group, with certain exceptions. the functional roles of iaps from other groups in the virus infection have not been well-established. we have previously shown that <em>hyphantria cunea</em> multicapsid npv (hycumnpv) encodes three <em>iap</em> genes, <em>hycu-iap1 ... | 2011 | 21795471 |
| Cloning and characterization of a dronc homologue in the silkworm, Bombyx mori. | We cloned and characterized a novel Bombyx mori homologue (bm-dronc) of Drosophila melanogaster dronc (dm-dronc), which could encode a polypeptide of 438 amino acid residues. Bm-Dronc shares relatively low amino acid sequence identities of 25% and 26% with Dm-Dronc and Aedes aegypti Dronc (Aa-Dronc), respectively. Bm-Dronc has the sequence QACRG surrounding the catalytic site (C), which is consistent with the QAC(R/Q/G)(G/E) consensus sequence in most caspases but distinct from the sequences PFC ... | 2011 | 21911060 |
| characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, spodoptera exigua (hübner) (insecta: lepidoptera: noctuidae). | to isolate antimicrobial-related genes from the beet armyworm, spodoptera exigua, we performed genefishing, a polymerase chain reaction (pcr)-based differential display technique. an attacin-like complementary dna (cdna) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. the full-length attacin cdna from s. exigua cdna (seattacin) was cloned using rapid amplification of cdna ends pcr. the attacin-like cdna transcript was 765 nucleot ... | 2011 | 22160467 |
| [electron microscopic study of single and double viral infections in the arctiid moth hyphantria cunea drury]. | | 2016 | 5028029 |
| the complete mitochondrial genome of the fall webworm, hyphantria cunea (lepidoptera: arctiidae). | the complete mitochondrial genome (mitogenome) of the fall webworm, hyphantria cunea (lepidoptera: arctiidae) was determined. the genome is a circular molecule 15 481 bp long. it presents a typical gene organization and order for completely sequenced lepidopteran mitogenomes, but differs from the insect ancestral type for the placement of trna(met). the nucleotide composition of the genome is also highly a + t biased, accounting for 80.38%, with a slightly positive at skewness (0.010), indicatin ... | 2010 | 20376208 |
| transcriptome and expression patterns of chemosensory genes in antennae of the parasitoid wasp chouioia cunea. | chouioia cunea yang is an endoparasitic wasp that attacks pupae of hyphantria cunea (drury), an invasive moth species that severely damages forests in china. chemosensory systems of insects are used to detect volatile chemical odors such as female sex pheromones and host plant volatiles. the antennae of parasite wasps are important for host detection and other sensory-mediated behaviors. we identified and documented differential expression profiles of chemoreception genes in c. cunea antennae. a ... | 2016 | 26841106 |
| genetic transformation and expression of transgenic lines of populus x euramericana with insect-resistance and salt-tolerance genes. | we characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. two insect-resistant bacillus thuringiensis (bt) genes, cry1ac and cry3a, and a salt-tolerant gene, betaine aldehyde dehydrogenase (badh) were inserted into a vector, p209-cry1ac-cry3a-badh. the clone of populus x euramericana was transformed by the vector using the agrobacterium-mediated method. three transgenic lines were assessed using genetic detection and resistance expression ... | 2016 | 27173305 |
| an improved pcr-restriction fragment length polymorphism (rflp) method for the identification of cry1-type genes. | the cry1-type genes of bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. it is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. in the present study, an improved pcr-restriction fragment length polymorphism (pcr-rflp) method which can distinguish 41 holotypes of cry1-type genes was developed. this improved method was used to identify cry1-type genes in 20 b. th ... | 2013 | 23995930 |
| molecular structure, chemical synthesis, and antibacterial activity of abp-dhc-cecropin a from drury (hyphantria cunea). | the increasing resistance of bacteria and fungi to currently available antibiotics is a major concern worldwide, leading to enormous efforts to develop new antibiotics with new modes of actions. in this paper, cdna encoding cecropin a was amplified from drury (hyphantria cunea) (dhc) pupa fatbody total rna using rt-pcr. the full-length dhc-cecropin a cdna encoded a protein of 63 amino acids with a predicted 26-amino acid signal peptide and a 37-amino acid functional domain. we synthesized the an ... | 2015 | 25241628 |
| srp gene is required for helicoverpa armigera prophenoloxidase activation and nodulation response. | srp gene was first identified from the fall webworm, hyphantria cunea as one of genes up-regulated after bacteria injection. a rent study in spodoptera litura showed that stress-induced elevation of srp expression highly correlates with reduced feeding activities and growth retardation of larvae. in this study, we identified a srp gene from the cotton bollworm, helicoverpa armigera, namely ha-srp, and studied its precise roles in insect immunity. expressions of ha-srp were upregulated in h. armi ... | 2014 | 24333441 |
| characterization of a novel chitinase, dkchi, from dendrolimus kikuchii nucleopolyhedrovirus. | dendrolimus kikuchii matsumura nucleopolyhedrovirus (dknpv) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against d. kikuchii. in this work, a 1755-bp dkchi gene with sequence homology to a chitinase gene was cloned from the genomic dna of dknpv using a dna fragment library. the dkchi gene, encoding 558 residues protein with a predicted mass of 61.6 kda, was expressed at high levels in escherichia coli and purified by affinity chromatography ... | 2013 | 23827975 |
| protective immunity of pichia pastoris-expressed recombinant envelope protein of japanese encephalitis virus. | japanese encephalitis virus (jev) envelope (e) protein holds great promise for use in the development of a recombinant vaccine. purified recombinant e (re) protein may be useful for numerous clinical applications; however, there are limitations in using the escherichia coli expression system for producing high-quality re protein. therefore, in this study, the yeast expression system was used to generate the re protein. for protein production using the yeast system, the full-length jev e gene was ... | 2012 | 23124351 |
| immunogenicity of japanese encephalitis virus envelope protein by hyphantria cunea nuclear polyhedrosis virus vector in guinea pig. | japanese encephalitis virus (jev) is an important pathogen causing febrile syndrome, encephalitis, and death. envelop (e) glycoprotein is the major target of inducing neutralizing antibodies and protective immunity in host. in this study, e glycoprotein of jev was expressed in spodoptera frugiperd 9 cells as a fusion protein containing a gx signal sequence of pseudorabies virus. this purified hce recombinant protein was evaluated for their immunogenicity and protective efficacy in guinea pig. th ... | 2012 | 22544685 |
| diapause induction, color change, and cold tolerance physiology of the diapausing larvae of the chouioia cunea (hymenoptera: eulophidae). | the chalcid wasp chouioia cunea yang (hymenoptera: eulophidae) is one of the most dominant pupal parasitoids of hyphantria cunea (drury) (lepidoptera: arctiidae), an invasive pest of many forestry trees and agricultural crops. for mass rearing c. cunea for biological control purposes, the pupae of antheraea pernyi (guérin-méneville) (lepidoptera: saturniidae) have been widely used as a substitute host in china. in this article, photoperiodic effect on diapause induction in c. cunea within the pu ... | 2014 | 25527599 |
| impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions. | poplar-cotton agro-ecosystems are the main agricultural planting modes of cotton fields in china. with increasing acres devoted to transgenic insect-resistant poplar and transgenic insect-resistant cotton, studies examining the effects of transgenic plants on target and non-target insects become increasingly important. we systematically surveyed populations of both target pests and non-target insects for 4 different combinations of poplar-cotton eco-systems over 3 years. transgenic bt cotton str ... | 2015 | 26345739 |
| p143 proteins from heterologous nucleopolyhedroviruses induce apoptosis in bm-n cells derived from the silkworm bombyx mori. | we previously demonstrated that ribosomal rna (rrna) of bombyx mori bm-n cells is rapidly degraded upon infection with heterologous nucleopolyhedroviruses (npvs), including autographa californica multiple npv (acmnpv), hyphantria cunea mnpv, spodoptera exigua mnpv and s. litura mnpv, and that this response is triggered by viral p143 proteins. the transient expression of p143 proteins from heterologous npvs was also shown to induce apoptosis and caspase-3-like protease activation in bm-n cells. i ... | 2017 | 28315706 |
| novel apoptosis suppressor apsup from the baculovirus lymantria dispar multiple nucleopolyhedrovirus precludes apoptosis by preventing proteolytic processing of initiator caspase dronc. | we previously identified a novel baculovirus-encoded apoptosis suppressor, apsup, from the baculovirus lymantria dispar multiple nucleopolyhedrovirus (ldmnpv). apsup inhibits the apoptosis of l. dispar ld652y cells triggered by infection with p35-defective autographa californica mnpv (vacĪ“p35) and exposure to actinomycin d or uv light. here, we examined the functional role of apsup in apoptosis regulation in insect cells. apsup prevented apoptosis and the proteolytic processing of l. dispar init ... | 2013 | 24067961 |
| degradation of rrna in bm-n cells from the silkworm bombyx mori during abortive infection with heterologous nucleopolyhedroviruses. | cell lines derived from the silkworm, bombyx mori, are only permissive for b. mori nucleopolyhedrovirus (npv), with other npvs generally resulting in abortive infection. here, we demonstrate that rrna of b. mori bm-n cells undergoes rapid degradation through site-specific cleavage upon infection with npvs from autographa californica (acmnpv), hyphantria cunea (hycumnpv), spodoptera exigua (semnpv) and spodoptera litura (spltmnpv). no significant decreases in cellular rna were observed in ld652y, ... | 2013 | 23784443 |
| resistance to bmnpv via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, bombyx mori. | the hycu-ep32 gene of hyphantria cunea npv can inhibit bombyx mori nucleopolyhedrovirus (bmnpv) multiplication in co-infected cells, but it is not known whether the overexpression of the hycu-ep32 gene has an antiviral effect in the silkworm, bombyx mori. thus, we constructed four transgenic vectors, which were under the control of the 39 k promoter of bmnpv (39 kp), bombyx mori a4 promoter (a4p), hr3 enhancer of bmnpv combined with 39 kp, and hr3 combined with a4p. transgenic lines were created ... | 2012 | 22870254 |
| baculovirus lymantria dispar multiple nucleopolyhedrovirus iap2 and iap3 do not suppress apoptosis, but trigger apoptosis of insect cells in a transient expression assay. | ld652y cells derived from the gypsy moth, lymantria dispar, are permissive for productive infection with l. dispar multiple nucleopolyhedrovirus (ldmnpv), but undergo apoptosis upon infection with various other npvs, including those isolated from bombyx mori, hyphantria cunea, spodoptera exigua, orgyia pseudotsugata, and spodoptera litura. in this study, we examined whether ldmnpv-encoded inhibitor of apoptosis 2 (ld-iap2) and 3 (ld-iap3) are involved in apoptosis suppression in ldmnpv-infected ... | 2012 | 22798056 |
| modulation of mnsod protein in response to different experimental stimulation in hyphantria cunea. | a manganese superoxide dismutase (mnsod) gene was cloned from the fall webworm, hyphantria cunea. mnsod cdnas encode precursor proteins of 215 amino acid residues. h. cunea mnsod possesses the metal binding ligands of 3 histidines and 1 aspartic acid common to mnsods. the deduced amino acid sequences of the h. cunea mnsod cdna showed 76% identity to bombyx mori mnsod and 56-62% identity to mnsod sequences from other species. mnsod and copper/zinc superoxide dismutase (cu/znsod) is expressed in a ... | 2010 | 20728562 |
| rapid detection of multiple nucleopolyhedroviruses using polymerase chain reaction. | a technique using the polymerase chain reaction (pcr) was developed for detection of the nucleopolyhedrovirus (npv) polyhedrin gene. the amino acid sequences of the polyhedrin gene were compared in twenty-six npvs. a highly conserved dna sequence within the coding region of the polyhedrin gene was targeted for amplification. one pair of degenerate pcr primers was designed to produce fragments of about 430 bp. the npvs detected by this technique were autographa californica npv, bombyx mori npv, h ... | 2001 | 11459223 |
| molecular cloning of chitinase cdnas from the silkworm, bombyx mori and the fall webworm, hyphantria cunea. | cdnas encoding chitinases were cloned and characterized from bombyx mori and hyphantria cunea, and their gene expression during the metamorphosis was also studied. the chitinase cdna from b. mori encodes a protein of 565 amino acids with a calculated molecular mass of 63.4 kda and the h. cunea chitinase cdna encodes a protein of 553 amino acids with a calculated molecular mass of 62.0 kda. amino acid alignment of the two chitinases revealed 75% homology and 77-80% with m. sexta chitinase. the pu ... | 1998 | 9654739 |
| role of proteases in extra-oral digestion of a predatory bug, andrallus spinidens. | roles of salivary proteases in the extra-oral digestion of the predatory bug, andrallus spinidens fabricius (hemiptera: pentatomidae) were studied by using 2% azocasein as a general substrate and specific protease substrates, as well as synthetic and endogenous inhibitors. it was found that salivary glands of a. spinidens have two anterior, two lateral, and two posterior lobes. azocasein was used to measure the activity of general proteases in the salivary glands using different buffer solutions ... | 2012 | 22954419 |
| juvenoids cause some insects to form composite cuticles. | metamorphosing insects treated with juvenoids may secrete composite cuticles which combine morphological features of two metamorphic stages within the area secreted by an individual epidermal cell. characters found combined were pigmentation, tanning, surface sculpturing, and microtrichiae. neighbouring cells frequently form different types of cuticle. composite cuticles should not be confused with the more common mosaic cuticles, which are composed of discrete areas with different stage-specifi ... | 1982 | 7153697 |
| two juvenile hormone suppressible storage proteins may play different roles in hyphantria cunea drury. | we isolated and sequenced cdna clones corresponding to two storage proteins (hcsp-1 and hcsp-2) from fall webworm, hyphantria cunea. the cdnas for hcsp-1 (2,337 bp) and hcsp-2 (2,572 bp) code for 753 and 747 residue proteins with predicted molecular masses of 88.3 and 88.5 kda, respectively. the calculated isoelectric points are pi = 8.4 (hcsp-1) and 7.6 (hcsp-2). multiple alignment analysis of the amino acid sequence revealed that hcsp-1 is most similar to sl-1 from s. litura (73.8% identity) a ... | 2002 | 12125057 |
| cdna sequence and gene expression of storage protein-2--a juvenile hormone-suppressible hexamerin from the fall webworm, hyphantria cunea drury. | we isolated and sequenced a cdna clone corresponding to storage protein-2 (sp-2) from the fall webworm, hyphantria cunea. the cdna for sp-2 (2572 bp) codes for a 747-residue protein with a predicted molecular mass of 88.5 kda. the calculated isoelectric point is 7.6. multiple alignment analysis of amino acid sequence revealed that sp-2 is most similar to bjhsp2 (74.3% identity). according to both the phylogenetic analyses and criteria for amino acid composition, sp-2 belongs to the subfamily of ... | 2001 | 11337253 |
| sequence analysis and expression of met-rich storage protein sp-1 of hyphantria cunea. | we isolated and sequenced a cdna clone corresponding to the 2.5 kb storage protein (sp-1) from fall webworm, hyphantria cunea. the sp-1 gene encoded a pre-protein of 753 amino acids, including a signal peptide of 15 amino acids. the deduced amino acid sequence of sp-1 contained one potential n-glycosylation site, and the calculated isoelectric point and molecular weight of secreted sp-1 were pi = 8.38 and 86.8 kda, respectively. a northern blot of mrna from various developmental stages revealed ... | 1998 | 9638655 |
| distribution and accumulation of storage protein-1 in ovary of hyphantria cunea drury. | storage protein-1 (sp-1) is a major storage protein found in the hemolymph and fat body of hyphantria cunea. in this study, the uptake and accumulation of sp-1 into the ovary of h. cunea was investigated using biochemical and immunocytochemical methods. sp-1 in h. cunea has a high methionine content (4.6%) but is not female-specific, like other high methionine storage proteins. in the 6-day-old pupal ovary, sp-1 was detectable in trace amounts but accumulated to significant levels toward the end ... | 1998 | 9435094 |
| comparative analysis of storage proteins of fall webworm (hyphantria cunea, drury). | two kinds of storage protein (sp-1 and sp-2) were purified from the hemolymph of last instar larvae of hyphantria cunea by ultracentrifugation, chromatofocusing, and ion-exchange chromatography. sp-1 and sp-2 each consist of six subunits and have the molecular mass of 79 kda and 82.5 kda, respectively. these showed different patterns of appearance in fat body and hemolymph, and different sds-page patterns in non-reducing gels. their stability in citrate-phosphate or tris-hcl buffers differs and ... | 1997 | 9418001 |
| antifeedant activity of ginkgo biloba secondary metabolites against hyphantria cunea larvae: mechanisms and applications. | ginkgo biloba is a typical relic plant that rarely suffers from pest hazards. this study analyzed the pattern of g. biloba pest hazards in beijing; tested the antifeedant activity of g. biloba extracts, including ginkgo flavonoids, ginkgolide, and bilobalide, against hyphantria cunea larvae; determined the activities of glutathione transferase (gsts), acetylcholinesterase (ache), carboxylesterase (care) and mixed-functional oxidase (mfo), in larvae after feeding on these g. biloba secondary meta ... | 2016 | 27214257 |