characterization of a caprine herpesvirus.a virus, which was isolated from kids (capra hircus) affected with a relatively severe generalized infection, was found to contain dna and to have a buoyant density of 1.2820 g/cm3. the virus was sensitive to the action of lipid solvents and trypsin and was rapidly inactivated at ph 3.0 and at temperatures of 50 and 56 c. the virion, an icosahedron consisting of a nucleoid surrounded by a double membrane, measured approximately 135 nm in diameter. on the basis of its chemical and physical proper ...1975933
metabolic characteristics of cells infected with a herpesvirus of turkeys.chicken embryo fibroblasts infected with the nononcogenic herpesvirus of turkeys (hvt) displayed an increased rate of glucose uptake, a pronounced alteration of the ph of the medium, and an increased production of lactic acid when compared to mock-infected cultures. objective estimates of cytopathology (quantifiable neutral red uptake and cell protein determination) showed that cell deterioration was a slow process in hvt-infected cells when compared to infection by herpes simplex virus. experim ...19751548
stable erythrocyte diagnostic preparation for passive haemagglutination test with herpes simplex virus antigen.a method for the preparation of stable suspensions of erythrocytes sensitized with herpes simplex virus (hsv) antigen and for their use in the passive haemagglutination test (phat) was developed. formolized sheep erythrocytes were treated with tannin and sensitized with hsv antigen prepared from infected chick embryo cell culture by ultrasonication and virus extraction with alkaline glycine buffer. antibody titres determined in the phat were higher than titres of neutralizing antibody. the speci ...197611670
purification and characterization of equine herpesvirus-induced dna. 197713532
varicella-zoster virus-induced dna polymerase.nuclear extracts of varicella-zoster virus (vzv)-infected human embryo lung (hel) cells were found to contain dna polymerase activity not present in uninfected hel cells. this enzyme was designated the vzv-induced dna polymerase. the vzv-induced polymerase was partially separated from the cellular alpha- and beta-polymerases by fractionation of the cells and by phosphocellulose chromatography. the separated enzymes were examined for the effect of added (nh4)2so4, activity with synthetic template ...197721226
nonstructural proteins of herpes simplex virus. i. purification of the induced dna polymerase.herpes simplex virus-induced dna polymerase purified by published methods was found to be contaminated with many others proteins, including virus structural proteins. thus, deae-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify dna polymerase from herpes simplex virus type 1- and type 2-infected cells. the purified enzyme retained unique features of the herpesvirus-induced dna polymerase, including a requirement for high salt concentrat ...197721304
measurement of antibodies to herpesvirus types 1 and 2 in human sera by microradioimmunoassay.the binding of 125i-labelled anti-human antibodies against the fc igg fragment to unlabelled antiviral immunoglobulins in the surface of infected cells was used to quantitate antibodies against herpes simplex virus type (hsv-1) and type 2 (hsv-2) in sera from patients with cervix carcinoma. the microradioimmunoassay technique (micro-ria) proved to be 5-10 times more sensitive than the microneutralization test. antibody titres determined by micro-ria correlated with neutralizing antibody titres t ...197722235
interactions between effector cell activity and lymphokines: implications for recovery from herpesvirus infections.the destruction of herpesvirus-infected target cells by antibody-dependent and direct cell cytotoxicity was enhanced by the presence of bovine lymphokine-containing preparations. to relate these effects to possible in vivo mechanisms of recovery, several in vitro approaches were used to measure the effects of lymphokine-containing preparations on controlling viral spread. in the first approach it was shown that in the presence of lymphokines, virus-infected cells could be killed earlier in the r ...197825244
failure of 5-bromodeoxyuridine to influence the activation of latent herpex simplex virus infection in explants of the rabbit regional sensory ganglia.the occurence of virus-specific antigens in explants of the gasserian ganglia of rabbits with healed keratitis induced by the kupka strain of human herpesvirus type 1 was tested by indirect immunofluorescence. in explants, the neurons and satellite cells revealed specific fluorescence from the 3rd day of culturing. the pattern of virus activation in the ganglion tissue was similar irrespective of whether the explants were cultured in the presence or absence of 5-bromo-2'-deoxyuridine.197827976
marke's disease herpesviruses. iii. purification and characterization of marek's disease herpesvirus b antigen.sera from chickens naturally infected with marek's disease herpesvirus (mdhv) form preciptin lines with at least two immunologically distinct soluble antigens designated mdhv-a and mdhv-b. partial purification and characterization of the glycoprotein mdhv-a antigen was previously reported. mdhv-b was found predominantly in the sonically treated extracts of infected cells, in contrast to the predominantly extracellular mdhv-a. analysis of these extracts from 14cglucosamine-labeled cells by immuno ...197829134
experimental pathogenesis of non-lethal herpesvirus infection and the establishment of rabbits inoculated into the right scarified cornea with the kupka strain of human herpesvirus type 1 (hhv 1), healing of the keratitis occurred between days 8--12 post infection (p.i.). viral particles were seen in nuclei and cytoplasm of corneal epithelium cells and in a few fibrocytes of corneal stroma. by immunofluorescence and electron microscopy, the virus was demonstrated in single neurons and satellite cells of the homolateral gasserian ganglion since 24 hr p.i. from 20 hr to 8 days p. ...197829466
autoantibodies in herpesvirus infections. 197829479
latent herpesvirus hominis 1 in the central nervous system of psychotic patients.cerebrospinal fluids (csf) from 35 patients with senile or presenile dementia and from 13 patients with schizophrenia and related syndromes were examined in cell cultures with the aim to isolate herpesvirus hominis 1 (hvh 1) or other viruses. serum and csf antibodies to hvh 1 and/or interferon in the patients indicated a recent hvh 1 antigenic or viral activity. in the csf of two senile demented patients and of one patient with schizoaffective psychosis, agents of low virulence, causing a cytopa ...197941436
a review of feline viral rhinotracheitis (feline herpesvirus i infection). 197941670
international symposium on anti-herpesvirus chemotherapy: experimental and clinical aspects. hamburg, september 7-9, 1978. 197943077
editorial: vaccination against an oncogenic herpesvirus. 197546967
hazards from simian herpes viruses: reactivation of skin lesions with virus shedding.a new simian herpes virus with biological properties similar to herpes simplex and to simian "b" virus has been used as a model system for studying virus latency in dorsal root spinal sensory ganglia. following intradermal injection, virus is present in the skin lesions and corresponding ganglia only, during the acute stage of the disease. by organ-culture techniques, latent virus was rescued from ganglia up to 2 years later. no latent virus was ever found in skin organ cultures of the primar ...197547078
chronic antigenic stimulation, herpesvirus infection, and cancer in transplant increased incidence of malignancy has been reported in transplant recipients. the pathogenesis of this increase was originally attributed to immunosuppressive therapy. however, not all tumours are increased in proportion to their occurrence in the general population-75% of reported tumours are lymphorproliferative or carcinoma of the skin, lip, or cervix. this cannot be explained by impaired immunosurveillance, and alternative hypotheses must be considered. 90% of transplant recipients develo ...197548901
no evidence for particles encapsulating rna-instructed dna polymerase and high molecular weight virus-related rna in herpesvirus induced tumours of non-human primates.the simultaneous detection test gave no evidence for the presence of rna tumour viruses in herpesvirus induced malignant lymphomas of non-human primates. the 12 tumours tested were obtained from three different monkey species inoculated with herpesvirus saimiri or herpesvirus ateles. particles encapsulating rna-instructed dna polymerase and high mol. wt. virus-related rna were easily demonstrated in tumours of the mouse induced by type-c or type-b oncornaviruses and in human lymphoid cells infec ...197549397
letter: herpesvirus infection and cancer. 197549591
defense mechanisms against bovine herpesvirus: relationship of virus-host cell events to susceptibility to antibody-complement cell lysis.the interaction of infectious bovine rhinotracheitis virus and susceptible host cells was examined to determine whether an infected cell could be destroyed by humoral immune mechanisms before or after the transmission of virus to susceptible adjacent cells. viral antigens were detectable on cell membranes at 6 h postinfection, but cells were not susceptible to antibody-complement lysis until 10 h postinfection. intracellular infectious virus was also detectable at 10 h postinfection, and transmi ...197553206
mechanism of phosphonoacetate inhibition of herpesvirus-induced dna polymerase.phosphonoacetate was an effective inhibitor of both the marek's disease herpesvirus- and the herpesvirus of turkey-induced dna polymerase. using the herpesvirus of turkey-induced dna polymerase, phosphonoacetate inhibition studies for the dna polymerization reaction and for the deoxyribonucleoside triphosphate-pyrophosphate exchange reaction were carried out. the results demonstrated that phosphonoacetate inhibited the polymerase by interacting with it at the pyrophosphate binding site to create ...197655273
relation between common antigen and membrane antigens associated with marek's disease herpesvirus and turkey herpesvirus infections.the appearance of two kinds of membrane antigen (ma) in marek's disease herpesvirus (mdhv) or herpesvirus of turkey (hvt) infected cells was examined using antiserum to the common antigen (common-ag). no early-appearing ma(ema) was detected in these cultures until 16 hours post-inoculation, after which a number of cells in these cultures had late-appearing membrane antigen (lma) as detected by immunofluorescence (if). fluids from cultures infected with these viruses were examined for the presenc ...197658652
morphological components of herpesvirus. ii. preservation of virus during negative staining procedures.the disruption of envelopes and the fragmentation of capsids of equine herpes-virus type i observed in negatively stained samples were attributed to viral dehydration on carbon films during preparation for electron microscopy. prior fixation of virus with oso4 or glutaraldehyde and subsequent application of negative stain before drying minimized envelope disruption and virtually eliminated the occurrence of capsomere sheets and broken capsids. this sample procedure significantly improves electro ...197658962
latency in herpesvirus hominis: its relationship to oncogenesis and recurrent disease. 197661629
a study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens.infectious adenovirus type 5 and coxsackievirus type b5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees c for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin g. lipid-containing herpes simplex virus type 1 and respiratory s ...197661971
[comparative antigenic characteristics of 2 marek's disease virus variants and of herpesvirus of turkeys isolated in the ussr].the antigens induced by pathogenic (55) and nonpathogenic (83) variants of marek's disease virus, the kekava strain (mdv-kekava) and herpesvirus of turkeys, the m-24-72 strain (hvt-m24-72) were tested in the agar gel precipitation test. the antigens were prepared from feather follicles (ff) of the infected chickens, from the infected cultures of chick embryo fibroblasts (cef) and from the culture fluid (cf) of these culttures. in the ff and cef cultures infected with mdv-kekava-83, the synthesis ...197664031
detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ...197764461
regulation of herpesvirus thymidine kinase activity in lm(tk) cells transformed by ultraviolet light-irradiated herpes simplex virus. 197765051
characterization of a new serotype of marek's disease herpesvirus.the hprs-24 strain of marek's disease herpesvirus was selected for closer study from a group of virus isolates that appeared apathogenic under standard test conditons. protection studies revealed an immunological relationship between this virus strain and acute marek's disease herpesvirus. in chicken kidney cell cultures, the hprs-24 strain caused small and slowly developing plaques, and the proportion of infected cells was less than with other strains. in chicken embryo fibroblast cultures, thi ...197566180
t-lymphoblastoid cell lines from marek's disease lymphomas.the establishment and continuous culture of two lymphoblastoid cell lines derived from marek's disease lymphomas is described. although the cells carried t-lymphocyte surface antigens, they had many features in common with cultured burkitt's lymphoma lymphoblasts, which carry b-cell determinants. a small proportion acted as infectious units in tissue culture, and a similarly small proportion contained intranuclear immature herpesvirus particles. the cells did not respond to phytohaemagglutinin. ...197566182
viruses and renal carcinoma of rana pipiens. xv. the presence of virus-associated membrane antigen(s) on lucké tumor cells.virus-specific antigens were detected in lucké tumor cells by indirect immunofluorescence using antiserum prepared against lucké herpesvirus. intracellular fluorescence, both cytoplasmic and nuclear, was observed only in acetone:methanol-fixed tumor cells that contained herpesvirus detected by electron microscopy. the number of positive cells correlated well with the number of cells containing virus. in contrast, both virus-containing and virus-free cells exhibited membrane fluorescence when via ...197766209
rapid detection of antibodies to cytomegalovirus by counterimmunoelectrophoresis.a new method for the detection of precipitating antibody to cytomegalovirus by counterimmunoelectrophoresis (ciep) is described. fourteen of 15 adult sera (95%) with igm-specific antibodies (as detected by immunofluorescence) and complement-fixing antibodies to cytomegalovirus in high titers gave positive reactions by this method. control sera from 156 patients and 40 normal subjects were negative by ciep. one of 32 individuals acutely infected with other members of the herpesvirus group gave a ...197766299
[demonstration of the bovid herpesvirus 2 in cell cultures with the aid of fluorescent antibodies].used was the immunofluorescence method employing fluorescinisothiocyanate-labeled antibodies against bovid herpesvirus 2. the localisation and the dynamics of the virus antigen were followed up in cell cultures of calf kidney epithelium. results showed that there was a specific cytoplastic-nuclear fluorescence in the infected cells. the dynamics of the virus multiplication revealed the presence of a specific antigen by about the 6th hour following the infection of the cells. discussed is the pro ...197667697
jejunal immunopathology in amyotrophic lateral sclerosis and multiple sclerosis. identification of viral antigens by immunofluorescence.jejunal mucosa from 7 patients with amyotrophic lateral sclerosis (a.l.s.), 20 newly reported patients with multiple sclerosis (m.s.), and 35 control patients without either disease was studied by immunofluorescence. an immune reaction was present in all a.l.s. specimens and consisted of altered ratios of immunoglobulin-labelled cells in the lamina propria, complement-labelled cells in the same location, and, in some, immunoglobulin and complement deposits in the epithelial basement membrane. po ...197768222
observations on the antigenic relationships between epstein-barr virus and herpesvirus saimiri.the antigenic relationships between epstein-barr (eb) virus and herpesvirus saimiri (hvs) have been investigated in comparative immunofluorescence, microimmunodiffusion and serum neutralization tests. no similarity was detected between the structural antigens of the two herpesviruses but the results of microimmunodiffusion tests showed that they shared a non-structural, virus-determined antigen. the implications of this finding are discussed in relation to the importance of hvs and its monkey ly ...197770507
detection of a nuclear, ebna-type antigen in apparently ebna-negative herpesvirus papio (hvp)-transformed lymphoid lines by the acid-fixed nuclear binding agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in herpesvirus papio (hvp)-transformed baboon lymphoid lines by anticomplementary staining in situ, as for ebna. however, by means of our recently developed acid-fixed nuclear binding technique an ebna-like antigen could be readily demonstrated, after extraction from both producer and non-producer lines. we propose to designate the antigen as hupna. it can be detected by a human anti-ebna ...197773523
characterization of an apparently nononcogenic marek's disease virus.a new isolate of marek's disease virus (mdv) was described. this virus, sb, and a clone, sb-1, differed from pathogenic isolates in in vitro growth characteristics as described for other apathogenic isolates. serologically, as with other apathogenic isolates, sb could be distinguished from pathogenic mdv and the avirulent turkey herpesvirus. sb failed to induce lesions characteristic of marek's disease (md) during a 6- to 11-week experimental period. also, sb was nononcogenic in immunosuppressed ...197876680
a phosphonoacetate-resistant mutant of herpesvirus of turkeys.a phosphonoacetate (pa)-resistant mutant of the herpesvirus of turkeys (hvt) was isolated and characterized. the mutant of hvt resistant to pa (hvtpa) replicated in duck embryo fibroblast (def) culture in media containing 300 microgram pa/ml, whereas the replication of the wild type of hvt (hvtwt) was completely inhibited in def culture in media containing 100 microgram pa/ml. the hvtpa was distinct from the hvtwt in plaque morphology, but was indistinguishable antigenically and showed in vitro ...197876681
cytomegalovirus d.n.a. and adenocarcinoma of the colon: evidence for latent viral infection.the cause of adenocarcinoma of the colon has not been proven and a viral association has not been reported with this disease. a sensitive biochemical technique for the detection of viral nucleic acid was used to determine whether one type of herpesvirus, namely a human strain of cytomegalovirus, was uniquely present in tumour tissue. 24 specimens of colon, obtained at surgery from 14 patients, were analysed by membrane cr.n.a.-d.n.a. hybridisation. 4 of 7 tumours of the colon were definitely pos ...197876890
an immunosuppressive factor in serum of rabbits lethally infected with the herpesvirus of bovine malignant catarrhal fever.virus-neutralizing antibody does not protect animals challenged with the herpesvirus of bovine malignant catarrh; therefore, other parameters of the immune response were investigated. in vitro transformation of peripheral blood lymphocytes with mitogens and specific antigens was monitored throughout the course of disease in lethally infected new zealand white rabbits. the whole-blood culture method was used. transformation with mitogens was normal throughout the incubation period but fell precip ...197877299
proteins of herpesvirus type 2. iii. isolation and immunologic characterization of a large molecular weight viral protein. 197878574
specificity of the blastogenic response of human mononuclear cells to herpesvirus antigens.peripheral blood mononuclear (pbm) cells from donors with a history of prior infection with herpes simplex virus, varicella-zoster virus, and/or cytomegalovirus were cultured for 2 to 8 days with glycine-extracted antigens prepared from these viruses and from infectious bovine rhinotracheitis virus. the proliferative response of pbm cells from all donors was specific during the first 6 days in culture. during this period the cellular immune responses of the seronegative donors were clearly diffe ...197878901
possible role of fc receptors on cells infected and transformed by herpesvirus: escape from immune cytolysis.receptors for the fc portion of nonimmune immunoglobulin g were demonstrated on b103 rat brain neuroma cells infected with herpes simplex virus type 1 (hsv-1) kos by a radioimmunoassay using 125i-labeled heat-aggregated fc fragments. immune f(ab')2 fragments specific for hsv antigens competed efficiently for fc binding sites, suggesting that the binding of fc fragments to infected cells is specific for viral cell-surface antigens. it has been suggested that the binding of immune complexes to fc ...197880379
herpesvirus type2: study of semen in male subjects with recurrent infections.semen from 30 healthy male subjects with recurrent infections with herpesvirus type 2 was obtained when subjects were free of lesions and surveyed by tissue culture for an infectious virus in an attempt to elucidate the transmission of this disease. inclusion bodies compatible with herpesvirus were found in tissue cultures of semen from 2 participants but an infectious virus could not be cultured directly from any sample. the data suggest that herpevirus type 2 is not ubiquitous in semen of male ...197881324
selective stimulation and differentiation of early antigens in lymphoblastoid cell lines producing epstein-barr-like viruses.comparisons of early antigens (ea) of ebv-related lymphotropic herpes virus of old world primates have proved difficult to accomplish because antigen levels are low in infected cells and the ratio of virus capsid antigen to ea is generally high. to overcome these difficulties, we have developed a procedure which combines antigen stimulation with inhibition of late virus functions and results in the selective stimulation of ea. using this procedure, we have examined the ea of ebv, herpesvirus pap ...197882609
isoprinosine: an overview.isoprinosine appears to have a relatively low degree of both acute and chronic toxicity in both rodent and nonrodent species. preclinical studies have demonstrated that isoprinosine can inhibit the growth of both dna and rna viruses as well as potentiate cell-mediated immune response both in vitro and in vivo. clinical studies with rhinovirus, herpesvirus, and influenza virus infections in man demonstrated that isoprinosine treatment reduced clinical symptoms and enhanced certain cell-mediated i ...197883189
immunological characterization of a common antigen present in herpes simplex virus, bovine mammillitis virus and herpesvirus simiae (b virus). 197887367
evidence for antigenic distinctness of the epstein--barr virus-determined nuclear antigen and the herpesvirus papio-determined nuclear antigen.previous serological evidence indicated that epstein--barr virus-determined nuclear antigen (ebna) has cross reactive components with the herpesvirus papio-determined nuclear antigen (hupna) and, in addition, it has distinct components of its own, not present in hupna. in the present study, this hypothesis was studied by absorption experiments. absorption of anti-ebna positive human sera with ebna abolished both anti-ebna and anti-hupna reactivity. absorption with hupna removed all anti-hupna re ...197988257
establishment of a lymphoblastoid cell line and isolation of an epstein-barr-related virus of gorilla origin.a b-lymphoid cell line was established from a normal gorilla. the cells contained epstein-barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. dna-dna reassociation kinétics revealed 30 to 40% hybridization to epstein-barr virus with 50 genomes per cell. examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed herpesvirus gorilla. h. gorilla transformed gibbon b-lymphocytes in vitro.197992573
parenteral acyclovir therapy for herpesvirus infections in man.acyclovir is a new antiviral agent which is highly active against herpesviruses in vitro and in laboratory animals. twenty-three cancer patients with cutaneous and/or systemic herpes zoster or herpes simplex infections were treated with parenteral acyclovir. all had received previous specific treatment for their malignant disease and ten had undergone bone-marrow transplantation. the drug seemed to arrest the progress of the infections and was most effective when given early. although two patien ...197993183
detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay.a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ...197994044
immune response to herpesvirus antigens in adults with acute cytomegaloviral mononucleosis.the immune response of eight patients with mononucleosis caused by cytomegalovirus (cmv) was measured early in their illness--when virus was present in their urine and/or blood--and subsequently during convalescence. levels of cmv-specific antibody rose early in the illness, but the proliferative response of mononuclear cells to cmv antigen did not reach the level characteristic of cmv-immune donors until several months later. the production of interferon by mononuclear cells in response to cmv ...197994336
fluorescence-activated separation of cervical abnormal cells using herpesvirus antigenic markers.antisera to total hsv-2 (g) viral antigens (ra-2) and to increasingly purified viral antigenic fractions ("crude" and "pure" ag-e) specifically stain hsv-2-(g)- or hsv-1-(f)-infected hep-2 cells in indirect immunofluorescence. anti-"crude" ag-e sera induced by hsv-1 (f) or hsv-2 (g) produce a single precipitin band of identity when reacted in gel immunodiffusion against soluble antigenic mixtures from hsv-2-infected cells (hsv-2 (g) sam). these reactivities are lost following adsorption of the s ...197994520
transformation of marmoset lymphocytes in vitro with herpesvirus ateles.circulating lymphocytes from three species of marmoset monkeys were transformed in vitro with herpesvirus ateles (hva) either by co-cultivation with lethally x-irradiated hva-producing lymphoblastoid cells or by infection with cell-free virus: 42 transformed lymphocyte cultures were obtained from 72 transformation attempts. attempts to transform squirrel monkey lymphocytes were unsuccessful in 29 attempts. association of hva with each transformed culture was demonstrated by staining of antigen-p ...197896033
organ culture model for the study of hvh-ii infections in carcinoma of the experimental model is described whereby human and monkey cervical tissues may be maintained as organ cultures for 21 and 40 days, respectively. inclusion of sodium carboxymethyl cellulose in the culture medium prolongs the survival time of tissues considerably. the sequential cytologic changes associated with herpesvirus hominis type ii (hvh-ii) infection are reported. these changes are considered in relation to the possible causal role of hvh-ii infection in cervical carcinogenesis.1979108642
co-carcinogenic events in herpesvirus oncogenesis: a review. 1978109391
herpesvirus papio: state and properties of intracellular viral dna in baboon lymphoblastoid cell lines.herpesvirus papio (hvp) is an indigenous b-lymphotropic virus of baboons (papio sp.) present in latent form in baboon lymphoblastoid cell lines. it shares cross-reacting viral capsid and early antigens with the epstein-barr virus (ebv), and hvp dna and ebv dna show partial sequence homology. ebv-specific complementary rna was employed here as a probe to investigate the physical state of the hvp dna component in baboon lymphoblastoid cells after fractionation of cellular dna by density gradient c ...1979113352
ultrastructural studies on the replication of herpes virus ateles-73 in owl monkey kidney cells.the replicative cycle of herpesvirus ateles, strain 73 (hva-73), was examined in the electron microscope and compared to that of other herpesviruses known to be oncogenic. a relatively slow replicative cycle of hva-73 in owl monkey kidney (omk) cells allowed us to distinguish cytoplasmic and nuclear stages of replication, comprising virus uptake, transport, maturation, and extrusion. virus uptake was observed within 10 hours of infection and occurred both as a result of fusion between virus and ...1979114147
production of cells and viruses in a new multiple-tube tissue culture propagator.a novel method for large-scale culture of anchorage-dependent cells is described. culture vessels were constructed by using roller bottles and pyrex tubing. parallel glass tubes, equally spaced by silicone rings and inserted into the roller bottles, provided an increased culture area. the roller bottles were fitted with a second opening for medium recirculation. culture vessels of 1,000-, 4,000-, 8,000- and 16,000-cm2 growth area were constructed to propagate vero, bs-c-1, primary chicken embryo ...1979115897
surface adhesive properties of baboon lymphocyte and derived lymphoblastoid cell lines.cell surface adhesiveness of lymphocytes isolated from the peripheral blood of papio hamadryas monkeys was compared to the adhesiveness of lymphoblastoid cell lines derived by transformation of these lymphocytes with herpesvirus papio. for the quantitative comparison of the cell surface adhesive properties, a recently developed latex particle adherence assay was utilized. it was found that cell surface adhesiveness of the suspension lines was substantially lower than that of their precursor lymp ...1979119650
virus infections in renal transplant recipients.534 serum samples from 73 renal transplant recipients, 41 haemodialysis patients, and 99 blood and organ donors were examined serologically for antibodies against cytomegalo, h. simplex (types 1 and 2), varicella-zoster, epstein-barr, adeno, influenza, parainfluenza, respiratory syncytial, measles, picorna- and human polyoma- viruses. serum specimens were stored in the lyophilized state until examined thus enabling a simultaneous testing of all samples belonging to one patient. all antigens, com ...1975126545
effects of lymphocytes from marek's disease-infected chickens on mitogen responses of syngeneic normal chicken spleen cells.pha responses have been measured in lymphoid cell cultures prepared by mixing normal chicken spleen cells with spleen or thymus cells from syngeneic chickens infected with the oncogenic herpesvirus mdv. results of these studies may be summarized as follows: 1) spleen cells from mdv-infected chickens with visceral lymphomas inhibit the pha response of normal spleen cells possibly by release of soluble inhibitory factors in response to the mitogen; 2) lymphoid cells from asymptomatic mdv-infected ...1977139449
herpesvirus induced feline urolithiasis--a review. 1979160299
invasion of the central nervous system by herpes simplex virus type 1 after subcutaneous inoculation of immunosuppressed mice.young adult mice given subcutaneous inoculations in the rear footpad with herpes simplex virus type 1 were found to be resistant to infection unless they were immunosuppressed with sublethal doses of irradiation or cyclophosphamide. in these mice, virus multiplied in the sciatic nerve three to four days after inoculation. this development was followed by multiplication of virus in the spinal cord and brain, which caused death of the mice in about eight days. no virus was detected in the blood or ...1975162925
cell-mediated immunity to herpes simplex in humans: lymphocyte cytotoxicity measured by 51-cr release from infected cells.we assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. this was demonstrated by lymphocyte transformation and specific 51cr release from infected human amnion cells after incubation with peripheal blood mononuclear cells. where performed, skin tests w ...1975163220
properties of a chicken lymphoblastoid cell line from marek's disease of a chicken lymphoblastoid cell line (msb-1) from a marek's disease tumor were studied. the cell line grew well at 41 degrees c in medium rpmi-1640 supplemented with 10% bovine fetal serum and had a doubling time of 8-12 hours. cells grown in stationary suspension culture did not attach to the vessel and had the morphology of typical lymphoblasts. at 37 degrees c, the cell line grew initially but ceased to divide after several subcultures. in the subcultures maintained for 48-72 hour ...1975163334
spontaneous lymphoma associated with herpesvirus saimiri in owl monkeys.two cases of lymphoma and one case of lymphoproliferative disease were found in a group of 7 owl monkeys imported into our colony as a single group. herpesvirus saimiri (hvs) was isolated from the tumor cells of 1 lymphoma by cocultivation and from kidney cell cultures from the monkey with lymphoproliferative disease. antibody to hvs was found in serum samples from 2 monkeys positive for hvs but not in the sera from the 4 clinically normal monkeys. antibody to epstein-barr virus-infected cells w ...1975163336
synthesis of proteins in cells infected with herpesvirus. x. proteins excreted by cells infected with herpes simplex virus, types 1 and 2. 1975163536
psittacine herpesvirus infection resembling pacheco's parrot disease.herpesvirus was detected by electron microscopy in hepatocytes of psittacine birds that died at a florida aviary. the virus was identified in hepatocytes of chick embryos and budgerigars that were given injections of liver suspensions from naturally infected psittacines. infected hepatocytes had prominent intranuclear inclusions that contained naked nucleocapsids. both naked and enveloped virions were seen in the cytoplasm of hepatocytes, and enveloped nucleocapsids occasionally were seen in the ...1975163868
horizontal transmission of turkey herpesvirus to chickens. iv. viral maturation in the feather follicle experimental line of white leghorns (wsu-vs) and a commerical line inoculated with turkey herpesvirus (hvt) at 1 or 8 weeks old did not differ in the occurrence of infectious cell-free hvt in their feather-tip preparations (ft-hvt). the birds examined at 2 weeks postinoculation (pi) were always positive for ft-hvt regardless of age at virus inoculation, dose of hvt inoculated, or genetic line of chickens, whereas only a few were positive at 4 weeks and none at 6 weeks pi.1975164176
genital herpesvirus infection. 1975164310
lysis of herpesvirus-infected cells by immune spleen cells.spleen cells from herpes simplex-infected mice have been shown to lyse 51cr-labeled virus-infected target cells. the cell-mediated lysis was shown to be antibody dependent but not involving adherent cells. lysis of infected cells by this mechanism may be one form of host defense in infection by some viruses.1975164406
in ovo assay for marek's disease virus and turkey herpesvirus.marek's disease virus (mdv) and the turkey herpesvirus (hvt) may be assayed on the chorioallantoic membrane (cam) of the chicken embryo after intravenous inoculation of chicken embryo fibroblasts (cef) or chicken blood leukocytes infected with these viruses. free hvt, mdv associated with marek's tumor cells, and lymphoblastoid cell lines derived from marek's tumors, may be assayed in the same way. the intravenous assay is quicker than the yolk sac assay and somewhat more sensitive than in vitro ...1975164413
pathogenic and serologic studies of japanese quail infected with jm strain of marek's disease herpesvirus.japanese quail were inoculated with marek's disease herpesvirus at 1 day of age, and a second group of the same age was infected by contact with inoculated quail. a third group raised in our laboratory as a source of hatching eggs was accidentally infected with marek's disease (md). all quail were observed and examined for periods up to 360 days; the usual clinical signs of md were not observed. however, the birds were susceptible to md infection because of the evidence that: 1) the md-specific ...1975164560
infection of capuchin monkeys (cebus albifrons) with herpesvirus saimiri.herpesvirus saimiri (hvs) induced persistent, clinically inapparent infections of long-term duration in capuchin monkeys (cebus albifrons). the infections were characterized by development of antibody to hvs-associated antigens and recovery of low levels of virus-genome-carrying lymphocytes in the peripheral blood. peripheral lymphocyte counts remained in low-normal to normal ranges and no physical signs of lymphoma were evident. prednisolone treatment caused immunosuppression in one monkey; thi ...1975164564
depression in lymphocyte response to general mitogens by owl monkeys infected with herpesvirus saimiri.the effect of herpesvirus saimiri (hvs) infection on the response of owl monkey lymphocytes to general mitogens was examined during the development of neoplastic disease. the reactivity of the lymphocytes was then correlated with the clinical condition of the infected monkeys and the content of virus rescued from the peripheral blood lymphocytes (pbl). eight monkeys developed lymphoma which, in six monkeys, was accompanied by lymphocytic leukemia. all animals that died of hvs-induced neoplasia c ...1975164565
effect of bacillus calmette-guérin immunization in marmosets infected experimentally with herpesvirus saimiri.eight white-lipped marmosets immunized with bcg and 3 sham-immunized marmosets were studied after inoculation with herpesvirus saimiri (hvs). bcg immunization had no significant influence on the incidence of infection by hvs, incidence of fatal malignant lymphoma, time of leukemia onset, development or titer of hvs antibodies, or average survival time. one bcg-immunized, hvs-infected marmoset failed to develop malignant lymphoma, whereas the remaining 10 hvs-infected marmosets died of malignant ...1975164566
thymidine kinase activity in cerebrospinal fluid of rabbits with herpesvirus hominis encephalitis.the rabbit model of herpesvirus hominis (hvh) encephalitis was utilized to determine whether thymidine (tdr) kinase (ec., an early enzyme in the hvh replicative cycle, is present in the cerebrospinal fluid (csf) of rabbits with hvh encephalitis. the mean tdr kinase activity in csf from infected rabbits was 2.30 plus or minus 1.69 pmol product/120 min/100 mul csf, while the mean tdr kinase activity of uninfected csf was 0.34 plus or minus 0.25. the enzyme appeared to be cell associated ...1975164648
immunity and the level of neutralization antibodies in foals and mares vaccinated with a modified live-virus rhinopneumonitis vaccine.foals (farms 1 and 2) and mares (farms 1 and 3) vaccinated with a commercially available rhinopneumonitis vaccine were tested for the level of antibodies and for protection against the natural infection. the serum-neutralization (sn) antibody titers against equine herpesvirus type 1 were 1:8 or less in the majority of foals and 1:16 or higher in about 10%. the level of the nasal secretory antibody in the foals (farm 2) was less than 1:4. the vaccinated foals did not show any apparent respiratory ...1975164808
ultrastructure of herpesvirus simiae (herpes b ivurs). 1975165095
studies on immunity to aujeszky's disease (pseudorabies) virus infection in pigs.aujeszky's disease is primarily a disease of pigs caused by a herpesvirus. in pigs, the primary site of virus replication is the nasopharynx. some strains cause lesions only in the central nervous system, others cause rhinitis, pneumonia and encephalitis. colostrum from immune sows protected 3 day old piglets from experimental infection, but did not prevent the piglets excreting virus. colostral antibodies were 11-16-fold greater than the serum titres in the sows and the half life of passiveanti ...1975165131
genital herpes: treatment with methylene blue and light exposure.fifty-six patients with genital herpes were treated by photodynamic inactivation of herpesvirus by methylene blue (36 patients), proflavine (101 or neutral red (10). the first 35 patients were randomly selected for treatment with one of the three dyes. all the others were initially treated with methylene blue. the disease was temporarily eradicated in about 70% of instances by exposure of the lesions to light plus topically applied methylene blue, neutral red or proflavine. relapses, however, we ...1975165157
specific immunity and nonspecific resistance to infection: listeria, protozoa, and viruses in mice and hamsters,.specific immunity developed by mice against protozoan (toxoplasma gondii and besnoitia jellisoni) and bacterial (listeria monocytogenes) infections was compared with nonspecific protection conferred by prior infections. the results indicated that homologous immunity protected mice from more than 10-5 ld50 of t. gondii or b. jellisoni, but from only 10-2 ld50 of l. monocytogenes. heterospecific protection among these organisms was for 10-0.4 minus 10-1.2 ld50. in studies in hamsters specific immu ...1975165241
replication of herpesvirus saimiri in cultured lymphocytes of infected owl monkeys (aotus trivirgatus). an electron microscopic and immunofluorescent study.the lymphocytes of five owl monkeys infected with herpesvirus saimiri (hvs) and three control monkeys were studied. lymphocytes were separated on ficoll-hypaque gradients, incubated in suspension cultures, and prepared for electron microscopic and immunofluorescent study at 24, 48, and 72 hours after the beginning of culture (ac). buffy coats of whole blood immediately after bleeding were also prepared for study. at the time of the study, four of the five infected monkeys had died of malignant l ...1975165331
etiology of nongonococcal urethritis.chlamydia trachomatis was isolated from the urethra from 48 (42 per cent) of 113 men with non-gonococcal urethritis (ngu), four (7 per cent) of 58 without overt urethritis, and 13 (19 per cent) of 69 with gonorrhea. postgonococcal urethritis (pgu) developed in 11 of 11 men who had c. trum antibody to c. trachomatisis developed. the immunotype specificity of chlamydial antibody corresponded to the immunotype isolated. among culture-negative patients. chlamydial antibody prevalence correlated with ...1975165407
regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral was previously shown that virus-specific polypeptides made in hep-2 cells infected with herpes simplex 1 form three groups designated alpha, beta, and gamma whose synthesis is coordinately regulated and sequentially ordered. this report shows that one or more functional alpha polypeptides are necessary to turn on the synthesis of beta and gamma groups, and conversely, one or more polypeptides in the latter groups turn off the synthesis of alpha polypeptides. specifically, infected cells maint ...1975165503
malignant transformation of hamster cells following infection with bovine herpesvirus (infectious bovine rhinotracheitis virus.hamster embryo cells, following infection with ibr virus, showed malignant transformation. hamsters of all ages, inbred or random bred, inoculated with two of the transformed cell lines developed solid tumors. preliminary characterization of the tumors induced by one of the cell lines has indicated undifferentiated sarcomas. viral specific antigen was detected in about 5% of the transformed cells and 10% of primary tumor cells in culture. viral specific antibody was detected in the serum of ...1975165538
tumor antigens in hamsters with sarcomas associated with herpesvirus type 2.tumor-associated antigens (tta) were demonstrated in preparations of hamster sarcomas associated with herpes simplex virus type 2, as well as in the sera of tumor-bearing hamsters. an immunoadsorption-in-gel method was employed to demonstrate and purify the taa and anti-taa. these results suggest the potential use of this technique for the demonstration of taa or of anti-taa in humans or animals with cancer.1975165539
a comparison in calves of the antigenicity of three strains of bovid herpesvirus 2.three strains of bovid herpesvirus 2, viz. allerton, bovine mammillitis and 69/1lo were used to infect calves intradermally. twenty-eight days later the immunity of the calves was challenged by intravenous injection of a homologous or heterologous strain. challenge control calves developed a fever (greater than 40 degrees c) lasting several days and widespread skin lesions which varied with the strain. homologous challenge of the primary infection produced neither skin lesions nor febrile respon ...1975165571
early functions of the genome of herpesvirus. v. serological analysis of "immediate-early" proteins. 1975165618
in vitro transformation of hamster cells by herpes simplex virus type 2 from human prostatic cancer cells.a cell-associated herpes simplex virus type 2 found in a human prostatic carcinoma induced in vitro transformation of hamster embryo cells. the transformed cells (yw-74) have been shown to be hamster cells by karyotype analysis. their epithelial morphology and growth pattern, which are different from the parental cell, have remained stable through cell passages. the presence of herpesvirus antigens in the transformed cells was determined by specific immunofluorescence and colony inhibition tests ...1975165889
clinicopathologic characterization of herpesvirus saimiri malignant lymphoma in new zealand white rabbits.twenty-two of 39 rabbits inoculated with herpesvirus saimiri developed malignant lymphoma and either died or were killed between 17 and 165 days after inoculation. no clinical signs were present in animals developing the disease before 46 days, but all other rabbits had a severe conjunctivitis, nasal discharge, and dyspnea resulting from a lymphocytic invasion of the ocular and nasal tissues. four rabbits developed terminal leukemia. pathologically, the disease resembled h. saimiri malignant lym ...1975166191
horizontal transmission of turkey herpesvirus to chickens. 3. transmission in three different lines of chickens.horizontal transmission of turkey herpesvirus (hvt) was studied in three separate trials using three different lines of chickens in each trial. there was no horizontal spread of hvt to contact cagemates through 8 weeks regardless of the line of donor chickens, when inoculated subcutaneously with 9.2 times 10-3 plaque-forming units (pfu) of hvt at 1 week of age. the virus spread poorly to a few cagemates from an experimental line of white leghorns (w.s.u.-v.s.), but not from a commercial line o ...1975166362
isolation of equine herpesvirus type 1 from a horse with an acute paralytic disease.a standardbred mare became paralyzed shortly after showing signs of an upper respiratory infection. the mare was euthanized and equine herpesvirus type 1 was isolated from the brain and spinal cord.1975166741
inhibition of herpesvirus hominis replication by human interferon.the effect of human interferon on single-cycle replication of herpesvirus hominis type 1 (hv-1) and vesicular stomatitis virus (vsv) in human foreskin fibroblast cultures (hff) was studied. after treatment of hff cultures with low concentrations (58 u) of human interferon, a variable but statistically significant inhibition of hv-1 was observed. at higher concentrations (greater than 95 u) yield reduction of hv in interferon-treated cultures approached that of vsv. preliminary data indicate that ...1975166922
effect of human leukocyte interferon on vaccinia-and herpes virus-infected cell cultures and monkey corneas.pretreatment of human fibroblast cultures with human leukocyte interferon (hif, 1,000 iu/ml) resulted in a 24-h delay of virus replication after infection with vaccinia virus and herpes simplex virus type 1 and type 2. additional hif treatment 24 h after infection effectively lowered the maximum yield of viral infectivity. equal results were obtained in simian cells with 3,000 iu of hif per ml. the spread of two cell-bound herpesviruses, varicella zoster virus and medical lake macaque herpesviru ...1975166924
herpersvirus strigis: host spectrum and distribution in infected owls.herpesvirus strigis, a new species of the genus herpesvirus, is a pathogen for several species of owls in the order srigiformes. natural infection has been observed in the eagle owl (bubo bubo l.), long-eared owl (asio otus l.) and snowy owl (nyctea scandiaca l.) in addition the little owl (athene noctua scopolic) and tengmalms owl (aegolius funereus l.) was experimentally infected. on the other hand the tawny owl (strix aluco l.) and barn owl (tyto albo scopoli) proved resistant to a massive ex ...1975167204
multiple herpesvirus simiae isolation from a rhesus monkey which died of cerebral infarction.a rhesus monkey housed in the new england regional primate research center for more than 4 yr died after an illness of 19 da. clinical signs included central nervous system involvement and ulcers on the labial mucosa. microscopically, the cause of death was established as multiple cerebral infarction. lesions characteristic of herpesvirus infections were not present in the brain nor in any other tissue; however, herpesvirus simiae was isolated from oral and anal swabs as well as from tongue and ...1975167229
iodination of herpesvirus nucleic acids.a simple method is described for the iodination of herpes simplex virus (hsv) dna. the procedure involved synthesis of 125-i-labeled 5-iodo-dctp which was subsequently used as a precursor for the in vitro repair synthesis of hsv dna. synthesis of 5-iodo-dctp and purification from oxidation and reduction reagents, buffer salts, unreacted dctp and na125-i was accomplished in a single chromatographic step. it was possible to prepare 125-i-labeled hsv dna in vitro with specific activities exceeding ...1975166201
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