| a survey on intestinal parasitic infections in laotian refugees at ubon province, northeastern thailand, with special reference to schistosomiasis. | 454 stool specimens were collected from the laotian refugees residing in the refugee camp, ubon province, from august 8 to 11, 1978, and were examined by formalin ether sedimentation technique. one of them (0.22%) was infected with schistosoma mekongi. since there are freshwater hydrobiid snails, lithoglyphopsis aperta, the intermediate host of s. mekongi in that area, it is probable that the disease may become established in northeastern thailand in the future, which will eventually lead to hea ... | 1979 | 575436 |
| schistosoma mekongi sp. n. from man and animals, compared with four geographic strains of schistosoma japonicum. | schistosoma mekongi sp. n. is described from man and animals in cambodia. it is compared to 4 geographic strains of schistosoma japonicum. it differs from s. japonicum in the size of embryonated eggs, in the length of the prepatent peroid in the mammalian host, and in its utilization of a different snail host. the relative usefulness of conventional morphologic criteria in the differentiation of asian schistosomes is discussed. | 1978 | 682060 |
| changes in tegumental surface of schistosoma mekongi voge, bruckner, and bruce 1978, in the mammalian host. | | 1978 | 722467 |
| schistosoma mekongi infection in a leiomyosarcoma of the small bowel: a case report. | we report a fortuitous occurrence of a small bowel leiomyosarcoma infected with schistosoma mekongi in an elderly patient from kampuchea. s mekongi is endemic in countries along the lower mekong river basin (laos, thailand, and kampuchea), where its intermediate snail hosts (lithoglyphopsis aperta) are found. the diagnosis was made by examining the numerous ova that were embedded in the tumor and along the small bowel wall. the ova with their small lateral spines are easily confused with those o ... | 1992 | 1563751 |
| [hepatic bilharziasis with schistosoma mekongi]. | | 1991 | 2070974 |
| [bilharziosis caused by schistosoma mekongi: diagnosis by rectal biopsy and treatment with praziquantel: report of 5 cases]. | | 1990 | 2289667 |
| [bilharziasis caused by schistosoma mekongi along the mekong and its affluents mun and tonle sap. apropos of 2 cases observed at the university hospital center of tours]. | the authors report two cases of schistosomiasis mekongi observed in laotian people living in touraine. discussions about epidemiology, clinical, biology and treatment are taken up. | 1986 | 3089638 |
| [schistosoma mekongi bilharziasis along the mekong river and its affluents, the mun and tonle sap rivers. general review]. | first cases of schistosomiasis in the south east asian were described 30 years ago but the existence of a particular species in this area: schistosoma mekongi has been discussed for a long time. during the last few years, emigration of laotian and cambodian people allowed a better knowledge of this affection. through recent literature the authors make a restatement of the epidemiology, biology and treatment of this disease. | 1987 | 3323768 |
| [treatment of bilharziasis due to schistosoma mekongi with praziquantel]. | after a review of the mechanisms of anti-schistosomal activity of praziquantel and the treatment of schistosoma mekongi with this drug, the authors report two cases of schistosoma mekongi treated with praziquantel with failure. rectal mucosa biopsies are performed and eggs are present. light microscopic, electron microscopic and on ultra-thin sections examination of these samples don't reveal morphological alterations of schistosoma mekongi eggs after treatment. | 1988 | 3367765 |
| scanning electron microscopic study of the tegumental surface of the hybrid schistosome between schistosoma mekongi and s. japonicum-like (malaysian). | hybridization experiments between the two non-sibling species of schistosomes, schistosoma mekongi in man and s. japonicum-like (malaysian) in rodents, were carried out. two laboratory-bred snail species, tricula aperta (beta race), the snail host of s. mekongi and robertsiella kaporensis, the snail host of s. japonicum-like (malaysian), were used for the production of cercariae. cross mating between s. mekongi and s. japonicum-like (malaysian) were achieved in the laboratory by the usual proced ... | 1987 | 3448774 |
| lung-migration patterns of schistosoma mekongi and s. spindale in mouse. | two groups of laboratory-bred swiss albino mice were used to study the lung-migration patterns of schistosoma mekongi and s. spindale. the first group was individually infected with 100 s. mekongi cercariae by hair-looping application on shaved abdomen. the latter group was individually exposed to 500 s. spindale cercariae by tail immersion. each group of these infected mice was then divided into subgroups. the number of schistosomulae was determined using a lung recovery assay starting from the ... | 1987 | 3448775 |
| topography and ultrastructure of the tegument of adult schistosoma mekongi. | the tegument of adult schistosoma mekongi has been studied by both scanning and transmission electron microscopy. the gross surface topography of the parasite resembles that of s. japonicum, in that branched ridges, microvilli and sensory papillae predominate; such characteristics distinguish these two species from the non-oriental schistosomes. a unique feature of s. mekongi, however, is the numerous pleomorphic protruberances which are concentrated particularly on the middle three-fourths of t ... | 1984 | 6096792 |
| modulation of the host response in human schistosomiasis. iv. parasite antigen induces release of histamine that inhibits lymphocyte responsiveness in vitro. | several mechanisms underlying the suppression of in vitro lymphocyte transformation responses to parasite antigens in human schistosomiasis have been previously described, but the role that immediate hypersensitivity reactions may have in regulating these lymphocyte transformation responses has been little explored. using hypaque-ficoll-separated peripheral blood mononuclear cells (pbmc) from patients with schistosome infections, we found that histamine release could be demonstrated routinely in ... | 1983 | 6185579 |
| specificity of antibody and cellular immune responses in human schistosomiasis. | thirty patients infected with schistosoma mekongi, s. mansoni or s. japonicum had cell-mediated immune responses assessed by lymphocyte transformation in vitro (lt), immediate hypersensitivity responses determined by basophil histamine release in vitro (hr) and igg antibody responses evaluated in an enzyme-linked immunosorbent assay (elisa). species specificity was evaluated with antigens obtained from adult worms or eggs of the three schistosome species. though cross-reactivity was present in a ... | 1983 | 6186153 |
| schistosoma mekongi infection in man: cellular immune responses and modulating mechanisms. | cell-mediated immune responses (cmi), as assessed by lymphocyte proliferation in vitro, were evaluated in 11 laotian patients harbouring asymptomatic chronic infections by schistosoma mekongi, a schistosome closely related to s. japonicum. when the mononuclear cells of these patients were cultured in autologous plasma, lymphocyte responses to schistosome antigens were essentially nil, not differing from those of unexposed north american controls. specific lymphocyte proliferation, however, was s ... | 1982 | 6212174 |
| three cases of schistosomiasis mekongi from northern laos. | three cases of intestinal schistosomiasis acquired in northern laos are reported. all three patients swam regularly for many years in the mekong river near the cities of vientiane and luang prabang. morphology of the eggs found on rectal biopsy indicated infection with schistosoma mekongi. one patient had severe portal hypertension with bleeding esophageal varices requiring a splenorenal shunt; studies of her liver biopsy showed s. mekongi eggs and periportal fibrosis. a second patient had abnor ... | 1984 | 6334449 |
| schistosomiasis mekongi diagnosed by rectal biopsy. | schistosoma mekongi, a parasite of the dog which is endemic in small foci along the mikong river, is rarely seen in europe. a case of schistosomiasis caused by s mekongi was recently seen in france, in a native of laos who had been living in thailand. he was admitted for treatment of previous diagnosed tuberculoid leprosy but was found to have hepatosplenomegaly, with a high eosinophil count and high bilirubin concentrations. serological examination suggested schistosomiasis, but there were no p ... | 1983 | 6409206 |
| treatment of schistosoma mekongi with praziquantel in cambodian refugees in holding centres in prachinburi province, thailand. | eighty-four cases of schistosomiasis mekongi among cambodian refugees in holding centres in thailand received praziquantel at 30 mg/kg body-weight orally twice in one day. those treated were admitted to hospital in order to observe side effects for 24 hours. assessment of the efficacy of praziquantel was based on cure rates. side effects observed consisted primarily of abdominal pain, anorexia, nausea, emesis and headache. these were generally mild and transient. physical signs revealed mild hep ... | 1984 | 6485054 |
| epidemiology and control of schistosomiasis in southeast asia. | schistosomiasis in southeast asia, caused by schistosoma japonicum, schistosoma mekongi and schistosoma japonicum-like, have been reported from six different countries. the s. japonicum infections are highly prevalent in the philippines with oncomelania hupensis quadrasi as the vector snail, and in localized areas in indonesia with o.h. lindoensis as the snail vector. the s. mekongi infections are recent discovery in the lower mekong basin in laos and kampuchea, with tricula aperta as the vector ... | 1984 | 6535256 |
| parasitic diseases with emphasis on schistosomiasis in cambodian refugees, in prachinburi province thailand. | epidemiologic surveys were carried on 24,619 cambodian refugees in khao-i-dang holding center, employing a skin test as a screening device to determine the prevalence of schistosomiasis. the diagnosis of schistosoma mekongi was confirmed in 62 by positive stool examination. the majority of the positive cases came from battambang province which is probably a new endemic area of schistosomiasis in cambodia. | 1983 | 6673125 |
| treatment of schistosoma mekongi with praziquantel: a double-blind study. | praziquantel is a newly developed drug effective in the treatment of schistosomiasis. in order to assess the efficacy and toxicity of praziquantel in the treatment of schistosoma mekongi, 11 infected laotian refugees, all belonging to a single extended family, were treated in a double-blind crossover trial with praziquantel and placebo. initially, one group of five received praziquantel 60 mg/kg body weight in three divided doses orally while the remainder received an identically appearing place ... | 1982 | 6751116 |
| ileal varices revealed by recurrent hematuria in a patient with portal hypertension and mekong schistosomiasis. | this present report describes a unique case of a patient with ileal varices revealed by recurrent bleeding from vesical varicosities. ileal varices were associated with portal hypertension secondary to portal thrombosis. an unusual ileovesical shunt was demonstrated by the venous phase of the superior mesenteric arteriogram. this shunt was favored by adhesions between an abnormal ileal loop, which was infiltrated with ova of schistosoma mekongi, and the superior vesical wall. the patient was tre ... | 1983 | 6859013 |
| comparative methods of infecting mice with schistosoma mekongi. | experiments were carried out to investigate the most suitable cercarial concentration and method of exposure for the experimental mice infected with cercariae of schistosoma mekongi. swiss albino mice of 2 months old were exposed individually by three methods exposure : abdominal skin exposure, tail immersion and subcutaneous injection. cercarial concentrations of 10 to 20 cercariae per mouse were used for each method of exposure. in general, it was observed that both the infection rate and tota ... | 1982 | 7112216 |
| prevalence of schistosomiasis and other parasitic diseases among cambodian refugees residing in bang-kaeng holding center, prachinburi province, thailand. | isolated cases of schistosomiasis were discovered on stool examination among cambodian refugees residing in thailand. further epidemiologic investigations were conducted on a sample of 5,085 cambodian refugees in the ban-kaeng holding center, using the intradermal skin test as a screening device to determine the prevalence of this disease. a positive diagnosis of schistosoma mekongi was confirmed in 17 of those examined by recovery of eggs in the stool. the prevalence of schistomiasis in the ban ... | 1982 | 7125065 |
| scanning electron microscopic study of the tegumental surface of adult schistosoma mekongi. | the surface topography of the tegument of adult schistosoma mekongi was studied by scanning electron microscopy (sem). in comparison to other species of human schistosomes the male tegument lacks tubercles and, except in the gynecophoral canal, also lacks spines; instead the surface is composed chiefly of trabeculae of highly perforated ridges which give it the 'spongy' appearance. in addition, there are 3 kinds of papillae interspersed on the surface among the ridges. the first is a doughnut-sh ... | 1982 | 7145474 |
| schistosomiasis in cambodian refugees at ban-kaeng holding centre, prachinburi province, thailand. | the epidemiologic investigation on schistosomiasis in 21,496 cambodian refugees residing at ban-kaeng holding centre was conducted by using the skin sensitivity test as a screening test. a positive diagnosis of schistosoma mekongi was confirmed in 74 of these cases through ova recovery in the stool. the resultant prevalence of schistosomiasis in ban-kaeng refugee holding center was 3.44 cases per 1,000 population. all of positive cases came from areas in cambodia where schistosomiasis has not be ... | 1982 | 7147003 |
| freshwater snails survey in and around the cambodian refugee camp, sakaeo district, prachinburi province, thailand. | the freshwater snails surveys were carried out during september 15, 1981 to december 15, 1981, especially for the intermediate or possible intermediate host of schistosoma mekongi in and around the cambodian refugee camp at ban kaeng holding centre, sakaeo district, prachinburi province. a total of 30 localities were visited and 15 species of freshwater snails were collected. the snails did not shed any cercaria of s. mekongi and none of these was the intermediate host of s. mekongi. | 1982 | 7170643 |
| infection with schistosoma mekongi in southeast asian refugees. | schistosoma mekongi is a newly recognized species of schistosoma that is closely related to schistosoma japonicum and is pathogenic to humans. like the other forms of schistosomiasis in which the parasites reside in the mesenteric vasculature, s. mekongi infection has hepatosplenomegaly and portal venous hypertension as its most common serious sequelae. schistosomiasis, although endemic in certain areas of southeast asia, has not been recognized as a health problem in asian refugees who are curr ... | 1981 | 7310175 |
| monoclonal antibodies against schistosoma mekongi surface tegumental antigens. | monoclonal antibodies were produced from naturally infected balb/c mice. thirteen hybridomas which were found to produce monoclonal antibodies against surface tegumental antigens of schistosoma mekongi by elisa assay were used in this study. the antigen specificities of hybridomas reactive with surface tegumental antigens were characterized and localized by immunoblotting analysis and avidin-biotin method. of the 13 hybridomas, only three produced monoclonal antibodies to the single epitopes in ... | 1993 | 7512753 |
| production and characterization of genus and species specific monoclonal antibodies against surface tegumental antigens of schistosoma mekongi. | a panel of monoclonal antibodies (moabs) against freeze-thaw surface tegumental antigens of schistosoma mekongi were produced from naturally infected balb/c mice. in this study, we have characterized two moabs which have different antigenic specificity for s. mekongi, s. japonicum and s. mansoni. the target epitopes of these two hybridoma antibodies are contained in the m(r) 38 kda (designated sme 38) and m(r) 97 kda (designated sme 97) proteins of adult worms as analysed by immunoblotting. the ... | 1994 | 7520153 |
| molecular phylogeny of schistosoma species supports traditional groupings within the genus. | phylogenetic relationships among 9 blood flukes (7 schistosome species, a spirorchid, and a sanguinicolid) were inferred from nucleotide sequences of the d1 domain of large subunit rrna and the v4 region of small subunit rrna. these sequences were more conserved than those examined by previous authors and thus may provide insight into deeper-level relationships. analyzed separately and combined by 3 methods, these data yielded congruent trees that were well supported by bootstrap resampling. the ... | 1996 | 8604099 |
| survival of beta and gamma races of neotricula aperta snail intermediate hosts of schistosoma mekongi in raw waters. | beta and gamma races of neotricula aperta were exposed to naturally raw waters of the sirindhorn reservoir, mun river and mekong river in laboratory conditions and non supplement on food. snails of two races could withstand well in variable qualities of water within 48 hours. the survival rates of n. aperta in three sources of raw water were 100% for the beta race and 97 to 100% for the gamma race. | 1996 | 9280010 |
| a small-scale survey of intestinal helminthic infections among the residents near pakse, laos. | a small-scale epidemiological survey was undertaken on the residents along the mekong river near pakse, laos, to know the status of helminthic infections. a total of 137 fecal samples were collected from the staffs of the provincial government, their family, and primary schoolchildren in pakse city, champassak province, and examined by kato-katz smear technique. the overall helminth positive rate was 75.9%, and the helminths detected were opisthorchis viverrini (43.8%). ascaris lumbricoides (26. ... | 1998 | 9529864 |
| clonning and sequence analysis of the 26 kda glutathiones-transferase gene of schistosoma mekongi. | the number of genomic dna or cdna sequences of schistosoma mekongi accessible in genbank or embl is very limited up to now. recently, two reports have appeared on the molecular phylogeny of schistosoma species inferred from partial sequence data of rrna genes; no further sequence data of s. mekongi is available yet. knowledge of the molecular structure of protein coding genes of s. mekongi will provide a better understanding of gene function in the genus schistosoma. a cdna library of s. mekongi ... | 1997 | 9561610 |
| infectivity of a cambodian isolate of schistosoma mekongi to neotricula aperta from northeast thailand. | all three strains of neotricula aperta (gastropoda: pomatiopsidae) sampled from populations in northeast thailand were found to be compatible with a schistosoma mekongi isolate from kratie district, eastern cambodia. the infection rates were: 3.0%, alpha-strain; 6.0%, beta-strain, and 20.5%, gamma-strain. the greater infectivity to the gamma-strain, over both the alpha- and beta-strains, was statistically significant. comparisons of infectivity patterns for the kratie isolate, with those describ ... | 1997 | 9705676 |
| foci of schistosomiasis mekongi, northern cambodia: ii. distribution of infection and morbidity. | in the province of kracheh, in northern cambodia, a baseline epidemiological survey on schistosoma mekongi was conducted along the mekong river between december 1994 and april 1995. the results of household surveys of highly affected villages of the east and the west bank of the river and of school surveys in 20 primary schools are presented. in household surveys 1396 people were examined. an overall prevalence of infection of 49.3% was detected by a single stool examination with the kato-katz t ... | 1999 | 10583901 |
| [other forms of schistosomiasis]. | schistosoma intercalatum: endemic in central africa, s. intercalatum is the causal agent of this intestinal bilharziasis, which is similar to mansoni's disease but with a characteristic lower localization (rectum and sigmoid). the principal clinical signs are digestive disorders: abdominal pain, diarrhea or dysentery, straining, tenesmus, rectal bleeding. the moderately enlarged liver is smooth and hard but not painful, especially observed in adolescents. oriental bilharziasis: schistosoma japon ... | 2000 | 11072381 |
| sex chromosome differences among the three races (alpha, beta, gamma) of the snail intermediate host of schistosoma mekongi, neotricula aperta. | chromosome analyses were performed on three races (alpha, beta, gamma) of neotricula aperta which were previously reported to show variation in the chromosome number and pairing in meiosis. we used an air-drying method, which was more reliable for chromosome preparations from fresh animal tissues than a squash method. each of the races had the same number of chromosomes, 2n=33 for males, and 2n=34 for females. the sex determination system was xo type (male: 32+x, female: 32+xx). the sex chromoso ... | 2000 | 11077260 |
| the pig as a host for schistosoma mekongi in laos. | a survey of helminths in domestic pigs was conducted in khong district, laos, to elucidate if these domestic animals could act as definitive hosts for schistosoma mekongi and to obtain a general overview of their helminthological infection status. fecal samples were collected from 98 pigs. twelve pigs (12.2%) were found to excrete s. mekongi eggs. infection was confirmed by detection of s. mekongi eggs in tissues of liver, rectum, and cecum of 2 pigs. a total of 75.8% of the pigs was infected wi ... | 2001 | 11426741 |
| schistosomiasis in the mekong region: epidemiology and phylogeography. | an account is given of progress made over the last 20 years in the study of mekong schistosomiasis, causative agent schistosoma mekongi (trematoda: digenea). emphasis is given to the discussion of work concerning the origin and subsequent dispersal of s. mekongi and related taxa, including relevant snails. the role of such phylogeographical data in schistosomiasis control and the prediction of areas at risk is examined. new palaeogeographical models are reviewed in relation to traditional explan ... | 2001 | 11757333 |
| epidemiology and control of mekongi schistosomiasis. | since the first case of schistosoma mekongi infection was reported in 1957, control measures have been implemented in laos and in cambodia. operational research provided the necessary information on parasite epidemiology and the associated morbidity in order to develop adequate control measures. s. mekongi transmission occurs in rocky banks of the river according to a seasonal cycle. common daily activities of villagers living in the endemic areas constitute the risk factors for infection. the p ... | 2002 | 12020888 |
| the first reported cases of canine schistosomiasis mekongi in cambodia. | we have been conducting surveys of schistosomiasis mekongi along the mekong river in cambodia since 1997. we attempted to detect canine schistosome infection during the survey in 2000 because dogs were reported to be natural reservoirs of the mekong schistosome in lao pdr. a total of 28 canine fecal samples were collected in kbal chuor village, kratie province and examined for schistosome eggs. one specimen had schistosome eggs (positive rate = 3.6%; egg density = 100/gram stool), which showed c ... | 2002 | 12693576 |
| first report of schistosoma mekongi infection with brain involvement. | we describe, to our knowledge, the first reported case of schistosoma mekongi infection with brain involvement. s. mekongi is a distinct species most closely related to schistosoma japonicum that is endemic in a defined area of the mekong river in laos and cambodia and characteristically associated with hepatosplenic disease. the patient had an excellent response to praziquantel therapy but required repeated courses of corticosteroid therapy to suppress recrudescent neurological symptoms. | 2004 | 14679467 |
| schistosomiasis mekongi: from discovery to control. | in the mekong river basin, the first case of schistosomiasis was reported in 1957. in the 1960s, endemic areas of the infection, of which profiles were similar to those of schistosomiasis japonica, were discovered in khong island, laos, to kratie province, cambodia. a new intermediate snail host; neotricula aperta was identified and the mekong strain of schistosome was elevated to a new species: schistosoma mekongi in 1978. baseline epidemiological surveillance was performed and schistosomiasis ... | 2004 | 15081945 |
| schistosomes in the xe kong river of cambodia: the detection of schistosoma mekongi in a natural population of snails and observations on the intermediate host's distribution. | a natural population of neotricula aperta (gastropoda: pomatiopsidae) in the xe kong river of cambodia was found to be infected with schistosoma mekongi--apparently the first time this parasite has been found in snails outside the mekong river. only 0.14% of the n. aperta collected were found infected. potential habitats for n. aperta were examined in selected rivers of central and southern laos and cambodia, so that the density of n. aperta at each site could be estimated. at survey sites where ... | 2004 | 15119968 |
| schistosoma mekongi: a prominent neutrophil chemotactic activity of egg antigen with reference to that of schistosoma japonicum. | schistosoma mekongi causes granulomatous lesions around eggs deposited in the liver with neutrophil-rich inflammatory reactions in the early stage of the egg laying. to define the aspects of the typical pathogenesis of s. mekongi infection, we determined the difference between soluble egg antigen (sea) from s. mekongi and s. japonicum with a focus on chemotactic factors for neutrophils or eosinophils. mean volume and protein amount of s. mekongi eggs was 71 and 58% of those of schistosoma japoni ... | 2005 | 15893311 |
| effects of praziquantel and artesunate on the tegument of adult schistosoma mekongi harboured in mice. | the effects of praziquantel and artesunate on the tegument of adult schistosoma mekongi harboured in mice were compared using scanning electron microscopy (sem). forty-two mice infected with s. mekongi for 49 days were treated intragastrically with either 300 mg/kg praziquantel or 300 mg/kg artesunate. mice were sacrificed 1 or 3 days post-treatment. worms were collected by perfusion and examined by sem. one to 3 days after administration of artesunate, the tegument of s. mekongi showed severe s ... | 2005 | 15925540 |
| an analysis of the calcium-binding protein 1 of fasciola gigantica with a comparison to its homologs in the phylum platyhelminthes. | a full-length cdna encoding the fasciola gigantica calcium-binding protein 1 (fgcabp1) was cloned from an adult stage cdna expression library in an immunoscreen using rabbit immune serum against the parasite's excretion/secretion antigens. the deduced amino acid sequence showed 96.3% identity to fh22cbp of fasciola hepatica. during development in the mammalian host fgcabp1 rna was detected in metacercariae, juveniles and adults and was exclusively localized to the tegumental cell bodies. immune ... | 2006 | 16297461 |
| schistosoma mekongi: the in vitro effect of praziquantel and artesunate on the adult fluke. | the efficacy and tolerance of 80 microg/ml praziquantel (pzq) and 40 microg/ml artesunate (ats) against adult stage schistosoma mekongi in vitro were investigated after 3, 6, 12, and 24h incubation by monitoring worm motility and compared tegumental changes using scanning electron microscopy (sem). thirty mice were infected with s. mekongi cercaria for 49 days. adult worms were collected by perfusion method and prepared for in vitro study. contraction and decreased motor activity were observed a ... | 2006 | 16413019 |
| [international conference on tropical medicine "medicine and health in the tropics" september 11 to 15 2005. bilharziasis caused by schistosoma mekongi: an almost eradicated endemic disease]. | | 2005 | 16465799 |
| opisthorchis viverrini: identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis. | a cdna encoding a novel eggshell protein (ovesp) with high-glycine (49.2%) and -tyrosine (27.8%) content was cloned from the human liver fluke opisthorchis viverrini. in the adult parasite, the rna products of the ovesp gene are limited to the vitelline follicles. they have a size of 800 nucleotides and are already present in 2-week-old juveniles. immune sera of hamsters, experimentally infected, and humans, naturally infected with o. viverrini, detect bacterially expressed recombinant ovesp (ro ... | 2006 | 16876169 |
| control of schistosoma mekongi in cambodia: results of eight years of control activities in the two endemic provinces. | in cambodia, schistosomiasis is transmitted in the provinces of kratie and stung treng where approximately 80000 individuals are estimated to be at risk of infection. the baseline prevalence of infection was estimated to be between 73% and 88%, and cases of severe morbidity (hepatosplenomegaly, puberty retardation) and mortality were very common. in 1994, the ministry of health of cambodia started schistosomiasis control applying universal chemotherapy with praziquantel (40mg/kg). the coverage o ... | 2007 | 17028047 |
| [histology of liver lesions due to schistosoma mekongi. about six cases with severe portal hypertension operated in cambodia]. | schistosomiasis mekongi was shown to be endemic, along the mekong river, in northern cambodia, affecting many patients with portal hypertension. surgical procedures were proposed to some patients with digestive haemorrhage history to avoid fatal recurrence. the aim of our study was to evaluate the intensity of the liver fibrosis among these patients. during surgical treatment, liver biopsies were collected, fixed in bouin or in formalin and processed at the institut pasteur of cambodia. sections ... | 2006 | 17253054 |
| [place of surgery in the prevention of recurrences of digestive haemorrhages at the patients presenting a portal hypertension due to schistosoma mekongi]. | in spite of a decrease of the prevalence of hepato-splenic schistosomiasis thanks to mass-treatment with praziquentel from december 1994 till now (cnm - msf - who - health provincial director) of target-populations in kratie province, severe cases of portal hypertension are not exceptional (digestive bleedings, after rupture of oesophageal varices). out of 106 cases of portal hypertension: ali patients have had clinical survey biological tests (liver function, haematology and serology). most of ... | 2006 | 17253055 |
| high susceptibility of neotricula aperta gamma-strain from krakor and sdau in cambodia to schistosoma mekongi from khong island in laos. | neotricula aperta gamma-strain snails collected from krakor and sdau in cambodia were found to have the same or higher susceptibility to schistosoma mekongi as n. aperta originally isolated from khong in laos. infection rates of n. aperta gamma-strain snails exposed to 3 miracidia at week 8 were: khong gamma-strain, 22.6%; krakor gamma-strain, 33.3%; and sdau gamma-strain, 67.4%. at week 10, the sdau gamma-strain showed the highest infection rate of 83.3%. we thus found significantly high suscep ... | 2007 | 17317276 |
| intestinal parasites in school-aged children in villages bordering tonle sap lake, cambodia. | the objective of this study was to study schistosoma mekongi and other intestinal parasitic infections, and intestinal symptoms and related complaints among school-age children and adolescents living around tonle sap lake. villages were selected where there were potential signs of schistosomiasis (hepatomegaly), and where subjects complained of intestinal symptoms. stool samples were collected from 1,616 children and were examined by kato-katz, saf concentration, and baermann technique; short cl ... | 2006 | 17333726 |
| schistosoma mekongi and schistosoma japonicum: differences in the distribution of eggs in the viscera of mice. | the difference in the distribution of schistosoma eggs in the viscera has not been clearly elucidated in the two closely related species schistosoma japonicum and schistosoma mekongi. in this study, we quantitatively compared the distribution of eggs in mice infected with the two species. in s. mekongi-infected mice, 56.6% to 69.4% of total eggs were found in the distal small intestine 9 to 15 weeks after infection, while in s. japonicum-infected mice, 48.8% to 71.8% of eggs were found in the pr ... | 2007 | 17521955 |
| liver morbidity due to schistosoma mekongi in cambodia after seven rounds of mass drug administration. | severe liver disease due to schistosoma mekongi was frequent in northern cambodia. between 1995 and 2002, seven rounds of mass chemotherapy (praziquantel) reduced infection from 50% to below 3%. in 2002, we assessed hepatosplenic morbidity by historical, clinical and ultrasonographic investigations in adults (older than 14 years) from endemic (n=342) and non-endemic (n=103) areas (kratie province). clinical hepatomegaly (25 vs. 0%), splenomegaly (55 vs. 0%), reported blood in stool (41 vs. 20%) ... | 2007 | 17568642 |
| making the most of mitochondrial genomes--markers for phylogeny, molecular ecology and barcodes in schistosoma (platyhelminthes: digenea). | an increasing number of complete sequences of mitochondrial (mt) genomes provides the opportunity to optimise the choice of molecular markers for phylogenetic and ecological studies. this is particularly the case where mt genomes from closely related taxa have been sequenced; e.g., within schistosoma. these blood flukes include species that are the causative agents of schistosomiasis, where there has been a need to optimise markers for species and strain recognition. for many phylogenetic and po ... | 2007 | 17570370 |
| dna-sequence variation among schistosoma mekongi populations and related taxa; phylogeography and the current distribution of asian schistosomiasis. | schistosomiasis in humans along the lower mekong river has proven a persistent public health problem in the region. the causative agent is the parasite schistosoma mekongi (trematoda: digenea). a new transmission focus is reported, as well as the first study of genetic variation among s. mekongi populations. the aim is to confirm the identity of the species involved at each known focus of mekong schistosomiasis transmission, to examine historical relationships among the populations and related t ... | 2008 | 18350111 |
| schistosomiasis in travelers and immigrants. | schistosomiasis is caused by trematode parasites of one of five species, schistosoma mansoni, schistosoma haematobium, schistosoma japonicum, schistosoma intercalatum, or schistosoma mekongi, acquired via skin contact with fresh water containing infectious larval cercariae. between 1 and 8 weeks after exposure, acute schistosomiasis presents as dermatitis, fever, fatigue, or eosinophilia. if untreated, long-term infection may cause anemia or undernutrition and may progress to liver fibrosis, por ... | 2008 | 18377815 |
| the evaluation of control measures against schistosoma mekongi in cambodia by a mathematical model. | we constructed a mathematical model for the transmission of schistosoma mekongi in cambodia. the simulation of the model will be instrumental in planning schistosomiasis control measures. the model includes two definitive hosts, humans and dogs, as animal reservoirs. dogs are recognized to play an important role in schistosomiasis transmission in cambodia. for the purpose of dealing with age-specific prevalence and intensity of infection, the human population was classified into eight age catego ... | 2008 | 18486538 |
| the distribution of mekong schistosomiasis, past and future: preliminary indications from an analysis of genetic variation in the intermediate host. | neotricula aperta is the only known intermediate host of schistosoma mekongi which infects humans in cambodia and the southern tip of lao pdr. dna-sequence data (partial rrnl, i.e., mitochondrial 16s large ribosomal-rna gene) were obtained for 359 n. aperta snails sampled at 31 localities in cambodia, lao pdr and thailand. a nested clade analysis was performed to detect and evaluate any geographical patterns in the observed variation and to identify genetic subpopulations or clades. coalescent s ... | 2008 | 18499512 |
| intracranial schistosoma mekongi infection. | | 2008 | 19205144 |
| modeling the dynamics and control of transmission of schistosoma japonicum and s. mekongi in southeast asia. | a mathematical model for transmission of schistosomes is useful to predict effects of various control measures on suppression of these parasites. this review focuses on epidemiological and environmental factors in schistosoma japonicum and schistosoma mekongi infections and recent advances in mathematical models of schistosoma transmission. | 2009 | 19290084 |
| travel-related schistosomiasis acquired in laos. | twelve israeli travelers acquired schistosomiasis in laos during 2002-2008, and 7 of them had acute schistosomiasis. the patients were probably exposed to schistosoma mekongi in southern laos, an area known to be endemic for schistosomiasis. four possibly were infected in northern laos, where reports of schistosomiasis are rare. | 2009 | 19891875 |
| immunoblot analysis of membrane antigens of schistosoma mansoni, schistosoma intercalatum, and schistosoma haematobium against schistosoma-infected patient sera. | antigens present in aqueous n-butanolic extracts (be) of schistosoma mansoni (venezuelan jl strain), schistosoma intercalatum (cameroon edea strain), and schistosoma haematobium (yemen strain) adult worm membranes were compared in immunoblot against sera of patients infected with s. mansoni, s. intercalatum, s. haematobium, schistosoma japonicum, or schistosoma mekongi looking for similarities (common antigens) and differences (species-specific antigens). about 17 s. mansoni be polypeptides (m ( ... | 2010 | 20333401 |
| the sensitivity of artesunate against schistosoma japonicum decreased after 10 years of use in china. | schistosomiasis due to schistosoma japonicum is a major public health problem in china. since 1995, artesunate has been used to treat and prevent schistosome infections in china. artesunate previously showed a high prophylactic efficacy against schistosome infection, with a protection rate of 100%. however, recent clinical trials and animal experiments have found that the sensitivity of many schistosomes to artesunate, including schistosoma mekongi and schistosoma mansoni, decreased. whether the ... | 2010 | 20549236 |
| characteristics of granuloma formation and liver fibrosis in murine schistosomiasis mekongi: a morphological comparison between schistosoma mekongi and s. japonicum infection. | a histopathological study was performed to clarify the characteristics of granuloma formation and liver fibrosis in schistosoma mekongi infection in comparison with s. japonicum infection. mice were exposed to s. mekongi (laotian strain) and s. japonicum (japanese strain) cercariae, and were dissected at 6, 8, 12, 16, and 20 weeks post-exposure. in the liver, granulomas in s. mekongi infection were cellular, initially organized with foam cells, and continuously appeared in the intralobular area, ... | 2010 | 20561395 |
| schistosoma mekongi in cambodia and lao people's democratic republic. | schistosomiasis found in communities along the mekong river in cambodia and lao people's democratic republic (lao pdr) is caused by the blood fluke schistosoma mekongi. early observations on patients in 1957 revealed severe intestinal and hepatosplenic disease. high mortality rates and widespread disease were reported from the provinces of northern cambodia (stung treng and kratié) and southern lao pdr (champasack) in the early 1970s and 1990s. control programmes built around mass drug administr ... | 2010 | 20624532 |
| efficacy of sodium metaperiodate (smp)-elisa for the serodiagnosis of schistosomiasis mekongi. | schistosomiasis mekongi is an important public health issue in endemic countries. in this study, we evaluated an indirect immunodiagnostic elisa method using schistosoma mekongi soluble egg antigen. sodium metaperiodate (smp)-elisa was utilized in order to remove the glycosylated epitopes responsible for false positive reactions and the results using this method were compared with those using conventional elisa (conv-elisa). forty-two serum samples from schistosomiasis mekongi egg-positive patie ... | 2011 | 21323161 |
| false-positive plasmodium falciparum histidine-rich protein 2 immunocapture assay results for acute schistosomiasis caused by schistosoma mekongi. | we report seven cases of false-positive plasmodium falciparum histidine-rich protein 2 (pfhrp2) malaria assay results in patients with acute schistosomiasis caused by schistosoma mekongi. pfhrp2 assays were negative in travelers infected with schistosoma mansoni or schistosoma haematobium (n = 13). malaria was ruled out and rheumatoid factor was negative in all patients. | 2011 | 21508157 |
| helminth and intestinal protozoa infections, multiparasitism and risk factors in champasack province, lao people's democratic republic. | detailed investigations of multiparasitism are scarce in the mekong river basin. we assessed helminth (trematode, nematode, and cestode), and intestinal protozoa infections, and multiparasitism in random population samples from three different eco-epidemiological settings in champasack province, southern lao people's democratic republic (lao pdr), and determined underlying risk factors. | 2011 | 21532735 |
| analysis of the effectiveness of control measures against schistosoma mekongi using an intra- and inter-village model in champasak province, lao pdr. | schistosomiasis mekongi is prevalent in the khong district of lao pdr, made up of one big island, khong, and numerous small islands in the mekong river. schistosoma mekongi is spread by neotricula aperta as the intermediate host along the mekong river. therefore, even if an epidemic of s. mekongi were stamped out in a certain village, infection may recur if the source of infection is a village located in the upper reaches of the mekong river. the purpose of this study was to construct a mathemat ... | 2011 | 21820079 |
| the complete mitochondrial genome of orientobilharzia turkestanicum supports its affinity with african schistosoma spp. | orientobilharzia turkestanicum is a blood fluke of many mammals and causes orientobilharziasis that is also a neglected parasitic zoonosis because the cercaria of o. turkestanicum can infect humans and cause cercarial dermatitis. the present study determined the complete sequence of mt genome of o. turkestanicum and revised its phylogenetic position based on mt gene content and arrangement. the complete mtdna sequence of o. turkestanicum was 14,755 bp in length, which is slightly larger than the ... | 2011 | 21930247 |
| infectious diseases of khmer immigrants in the united states: review of published reports. | infectious diseases are influenced by where patients have lived or travelled in the past, e.g., infection with schistosoma mekongi can be acquired during freshwater contact in cambodia, but not in the united states. here the infectious diseases of khmer immigrants in the united states were studied by reviewing published reports. thirteen case series and 9 case reports of 5,222 patients were identified. most reports were of infections with gastrointestinal parasites (8, 36%), plasmnodium species ... | 2011 | 23272458 |
| current status of human hookworm infections, ascariasis, trichuriasis, schistosomiasis mekongi and other trematodiases in lao people's democratic republic. | soil-transmitted helminth (sth) infections, and schistosomiasis and other trematodiases often have a high prevalence in developing countries. here, we present updated information on the prevalence of these parasites in the lao people's democratic republic (lao pdr) in 2012, arising from the annual national helminthiasis survey. fecal specimens were collected from 8,610 inhabitants of 12 provinces and one municipality (bokeo, houaphan, luang namtha, luang prabang, oudomxay, and phongsaly province ... | 2014 | 24515942 |
| first molecular identification and report of genetic diversity of strongyloides stercoralis, a current major soil-transmitted helminth in humans from lao people's democratic republic. | strongyloidiasis is a major soil-transmitted helminth (sth) disease that affects people worldwide. we present updated data on prevalence in the lao people's democratic republic (lao pdr) in 2015, arising from a community cross-sectional helminthiasis survey. fecal samples were collected from 327 individuals across three provinces in lao pdr (luang prabang in the north, khammouane in the center, and champasack in the south). agar plate culture and kato-katz methods were used to examine duplicate ... | 2016 | 27083185 |
| epidemiology of strongyloides stercoralis on mekong islands in southern laos. | strongyloides stercoralis is a neglected helminth infection potentially that can lead to systemic infection in immunocompromised individuals. in lao people's democratic republic (lao pdr, laos), information on s. stercoralis infection is scarce. we assessed s. stercoralis infection and associated risk factors and symptoms on the mekong islands in southern laos. baermann and kato-katz techniques were performed on two stool samples from each individual to detect s. stercoralis larvae and concomita ... | 2015 | 25291044 |
| repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a cross-sectional survey in southern lao people's democratic republic. | intestinal parasitic infections are common in lao people's democratic republic (lao pdr). we investigated the accuracy of the kato-katz (kk) technique in relation to varying stool sampling efforts, and determined the effect of the concurrent use of a quantitative formalin-ethyl acetate concentration technique (fect) for helminth diagnosis and appraisal of concomitant infections. the study was carried out between march and may 2006 in champasack province, southern lao pdr. overall, 485 individual ... | 2015 | 25225157 |
| schistosoma mekongi cathepsin b and its use in the development of an immunodiagnosis. | schistosomiasis mekongi is one of the most important human parasitic diseases caused by schistosoma mekongi in south-east asia. the endemic area is the mekong river sub-region from laos to cambodia. this parasite also infects dogs and pigs which are its alternative host species. currently, the lack of reliable rapid diagnosis makes it difficult to monitor the infection and spreading of the disease. in this study, we screened the antigens of the parasite with sera of infected mice using western b ... | 2016 | 26655041 |
| malacological investigation of the fully operational nam theun 2 hydroelectric dam project in khammouane province, central lao pdr. | we conducted a malacological investigation in four districts of the nam theun 2 (nt2) hydroelectric dam project area, khammouane province, central lao pdr (nakai, gnommalath, mahaxai and xe bang fai), after the first and second years of full operation in march 2010 and november 2011 to determine health risks for humans. a total 10,863 snail specimens (10 families/23 species) from 57 sampling stations and 12,902 snail specimens (eight families/21 species) from 66 sampling stations were collected ... | 2015 | 26863858 |
| perceived illness drives participation in mass deworming campaigns in laos. | multiple helminth infections are very common in communities of southern laos. preventive chemotherapy in combination with health education is the mainstay of control. we assessed the knowledge, perceptions and practices of rural communities related to endemic helminthiasis and their control during a mass drug administration (mda) campaign. short interviews with household heads (n=192), direct observations and discussions with attendees of mdas, and in-depth interviews with local opinion leaders ... | 2015 | 24713201 |
| multiparasitism and intensity of helminth infections in relation to symptoms and nutritional status among children: a cross-sectional study in southern lao people's democratic republic. | the occurrence and spatial distribution of intestinal helminth infection in children is fairly well understood. however, knowledge on how helminth infections govern intestinal morbidity is scarce. we conducted a cross-sectional study to assess and quantify the relationship between single and multiple species helminth infection with clinical and self-reported morbidity indicators and nutritional status in champasack province, southern lao people's democratic republic (lao pdr). a random sample of ... | 2015 | 25291046 |
| efficacy of praziquantel against schistosoma mekongi and opisthorchis viverrini: a randomized, single-blinded dose-comparison trial. | schistosomiasis and opisthorchiasis are of public health importance in southeast asia. praziquantel (pzq) is the drug of choice for morbidity control but few dose comparisons have been made. | 2012 | 22848766 |
| severe morbidity due to opisthorchis viverrini and schistosoma mekongi infection in lao people's democratic republic. | we assessed morbidity due to opisthorchis viverrini and schistosoma mekongi infections in 243 individuals in lao people's democratic republic. morbidity was associated with o. viverrini infection intensity. coinfection with s. mekongi resulted in excess risk of liver fibrosis and left liver lobe enlargement. the high public health impact of opisthorchiasis warrants control. | 2012 | 22670046 |
| diagnostic issues of acute schistosomiasis with schistosoma mekongi in a traveler: a case report. | a belgian traveler returning from laos developed acute schistosomiasis. feces microscopy and polymerase chain reaction (pcr) followed by sequence analysis revealed schistosoma mekongi. schistosome antibody test results and real-time pcr in serum were initially negative or not interpretable. a hrp-2 antigen test for plasmodium falciparum and an enzyme-linked immunosorbent assay (elisa) antibody test for trichinella yielded false-positive results. | 2013 | 23992575 |
| rapid detection and identification of four major schistosoma species by high-resolution melt (hrm) analysis. | schistosomiasis, caused by blood flukes belonging to several species of the genus schistosoma, is a serious and widespread parasitic disease. accurate and rapid differentiation of these etiological agents of animal and human schistosomiasis to species level can be difficult. we report a real-time pcr assay coupled with a high-resolution melt (hrm) assay targeting a portion of the nuclear 18s rdna to detect, identify, and distinguish between four major blood fluke species (schistosoma japonicum, ... | 2015 | 26253799 |
| comparative evaluation of schistosoma mansoni, schistosoma intercalatum, and schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (apia). | to know if alkaline phosphatase (ap) from schistosomes other than schistosoma mansoni can be used as diagnostic marker for schistosomiasis in alkaline phosphatase immunocapture assay (apia), we comparatively tested n-butanol extracts of adult worm membranes from a venezuelan (jl) strain of s. mansoni (ven/awbe/sm); a cameroonian (eden) strain of schistosoma intercalatum (cam/awbe/si) and a yemeni strain of schistosoma haematobium (yem/awbe/sh). apia was evaluated with sera of patients from venez ... | 2014 | 24452918 |
| schistosomiasis. | schistosomiasis is a parasitic infection caused by flatworms (trematodes). it is second only to malaria in public health significance, with over 200 million people infected worldwide, leading to severe consequences in 20 million persons and 100,000 deaths, annually. there are four species that cause intestinal schistosomiasis: schistosoma mansoni; schistosoma japonicum, schistosoma mekongi, and schistosoma intercalatum. schistosoma haematobium causes urinary schistosomias, and is endemic in afri ... | 2013 | 24079113 |
| human schistosomiasis: clinical perspective: review. | the clinical manifestations of schistosomiasis pass by acute, sub acute and chronic stages that mirror the immune response to infection. the later includes in succession innate, th1 and th2 adaptive stages, with an ultimate establishment of concomitant immunity. some patients may also develop late complications, or suffer the sequelae of co-infection with other parasites, bacteria or viruses. acute manifestations are species-independent; occur during the early stages of invasion and migration, w ... | 2013 | 25685450 |
| evaluation of banked urine samples for the detection of circulating anodic and cathodic antigens in schistosoma mekongi and s. japonicum infections: a proof-of-concept study. | in asia, schistosoma japonicum is the predominant schistosome species, while schistosoma mekongi is confined to limited foci in cambodia and lao people's democratic republic. while the people's republic of china has been successful in controlling schistosomiasis, the disease remains a major public health issue in other areas. in order to prioritise intervention areas, not only accurate diagnosis is important but also other factors, such as practicality, time-efficiency and cost-effectiveness, si ... | 2015 | 25225158 |
| molecular differentiation of schistosoma japonicum and schistosoma mekongi by real-time pcr with high resolution melting analysis. | human schistosomiasis caused by schistosoma japonicum and schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of asia. due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. high resolution melting (hrm) real-time pcr is developed as an alternative tool for the detection and differentiation of these 2 species. a primer pair was designed for targeting the 18s ribosomal rna gene to ... | 2013 | 24516269 |
| towards improved diagnosis of neglected zoonotic trematodes using a one health approach. | reaching the goal of control, elimination and eradication of the neglected tropical disease in a foreseeable future provides significant challenges at the ground level especially regarding helminthiasis. helminths are still mainly diagnoses by egg identification in stool, methods with low sensitivity and for most species low specificity. cross-sectoral collaboration with regard to zoonoses is almost non-existing and cross-validation by inter-laboratory evaluation of diagnostic tests is not a com ... | 2015 | 23886849 |
| schistosoma mansoni antigen detects schistosoma mekongi infection. | northern cambodia and southern laos are highly endemic for schistosoma mekongi. however, there is currently no immunological assay available that is specific for this form of schistosomiasis. we have validated schistosoma mansoni antigens to detect s. mekongi-directed antibodies in human sera collected from a highly s. mekongi endemic region in laos. on two consecutive days stool samples of 234 individuals were analyzed by kato-katz for presence of s. mekongi eggs and the results were correlated ... | 2015 | 25116398 |
| a new population and habitat for neotricula aperta in the mekong river of northeastern thailand: a dna sequence-based phylogenetic assessment confirms identifications and interpopulation relationships. | neotricula aperta (gastropoda: pomatiopsidae), the snail intermediate host of schistosoma mekongi, is found in cambodia, laos, and thailand. we update information on the distribution of this species in the mekong river and its tributary, the mun river, in thailand. dna sequences of a portion of the mitochondrial cytochrome c oxidase subunit 1 were obtained from n. aperta collected from different locations and used to confirm species and strain identities. specimens of the β-strain were found in ... | 2015 | 25487731 |
| chronic schistosoma mekongi in a traveler--a case report and review of the literature. | travel-related schistosomiasis can be detected in patients without symptoms of acute or chronic infection. a case of schistosoma mekongi acquired in an endemic area of laos and discovered unexpectedly from colon biopsies taken 5 years after infection is presented here. a literature review of previous cases of s. mekongi infection specifically associated with travelers is then presented. | 2014 | 24912375 |
| a phylogeny for the pomatiopsidae (gastropoda: rissooidea): a resource for taxonomic, parasitological and biodiversity studies. | the pomatiopsidae are reported from northern india into southern china and southeast asia, with two sub-families, the pomatiopsinae (which include freshwater, amphibious, terrestrial and marine species) and the freshwater triculinae. both include species acting as intermediate host for species of the blood-fluke schistosoma which cause a public health problem in east asia. also, with around 120 species, triculine biodiversity exceeds that of any other endemic freshwater molluscan fauna. neverthe ... | 2014 | 24548800 |
| a population growth trend analysis for neotricula aperta, the snail intermediate host of schistosoma mekongi, after construction of the pak-mun dam. | the pak-mun dam is a controversial hydro-power project on the mun river in northeast thailand. the dam is sited in a habitat of the freshwater snail neotricula aperta, which is the intermediate host for the parasitic blood-fluke schistosoma mekongi causing mekong schistosomiasis in humans in cambodia and laos. few data are available which can be used to assess the effects of water resource development on n. aperta. the aim of this study was to obtain data and to analyze the possible impact of th ... | 2013 | 24244775 |
| development of a real-time pcr assay with fluorophore-labelled hybridization probes for detection of schistosoma mekongi in infected snails and rat feces. | schistosoma mekongi, a blood-dwelling fluke, is a water-borne parasite that is found in communities along the lower mekong river basin, i.e. cambodia and lao people's democratic republic. this study developed a real-time pcr assay combined with melting-curve analysis to detect s. mekongi in laboratory setting conditions, in experimentally infected snails, and in fecal samples of infected rats. the procedure is based on melting-curve analysis of a hybrid between an amplicon from s. mekongi mitoch ... | 2012 | 22717071 |