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a prelude to the bugle call "taps": born in an army field hospital on a civil war battlefield. 197897595
vasoconstrictor activity of 8-o-acetylharpagide from ajuga reptans.the traditional therapeutic indications for the use of ajuga reptans (labiatae) have been investigated. the h2o-soluble part of a crude and partially purified meoh extract and two isolated iridoids (8-o-acetylharpagide and harpagide), were tested for a biological activity on isolated smooth muscle preparations from guinea pig.19921431938
["cholos de coclé": determination of their racial mixture and genetic origins].we have found, in this first genetic study of a supposedly admixed panamanian population, that the cultural group known as "cholos of coclé" constitute a trihybrid mixture, whose genetic pool has the following composition: 44% amerindian, 38% caucasoid and 18% negroid. similarly, we have detected amerindian genes, such as ldhb--gua and tfchi, in proportions that relate this population with the extant ngawbé (guaymí). nevertheless, the very high frequency of variant pepa--kun seems to indicate th ...19921439003
the task force report on tort reform: a bugle call for drastic changes. 19873655690
the ring-down artifact."ring-down" is an ultrasound artifact that appears as a solid streak or a series of parallel bands radiating away from abdominal gas collections. using an in vitro system of bubbles in water or gelatin, it was found that the ring-down artifact originated from the center of a cluster of four bubbles (bubble tetrahedron), three on top and one nestled beneath. entrapped between the bubbles is a horn- or bugle-shaped fluid collection that we theorize emits a continuous sound wave back to the transdu ...19853882979
[preparation of partial removable plate and bugle prosthesis with a disguised spring-elastic clasp attachment]. 19724560995
measles among members of a drum and bugle corps--arkansas, california, kansas. 19836415387
assessing maximal static ventilatory muscle pressures using the "bugle" dynamometer. suggestion from the field. 19846494258
triacylated anthocyanins from ajuga reptans flowers and cell cultures.four anthocyanins were isolated from ajuga reptans flowers and one from the cell cultures. by fab mass spectrometry measurements, the structures of these pigments were determined as delphinidin and cyanidin glucosides acylated with two cinnamic acids, while three of them were also malonylated. a delphinidin-based pigment in the crude extract from cell cultures was identical to the major flower pigment as shown by hplc co-chromatography. moreover, by application of 1h and 13c nmr consisting of dq ...19968728066
8-o-acetylharpagide is a nonsteroidal ecdysteroid agonist.we have identified a novel nonsteroidal ecdysteroid agonist. this compound was isolated from a methanol extract of ajuga reptans l. (lamiaceae) and the structure was identified by spectroscopic methods as 8-o-acetylharpagide. we have characterised this compound as an ecdysteroid agonist in a transactivation assay using beta-galactosidase as the reporter gene regulated by ecdysteroid response elements. in this assay, 8-o-acetylharpagide has an ec50 of 22 microm. the compound also competes with tr ...19968969463
biosynthesis of 20-hydroxyecdysone in ajuga hairy roots: fate of 6alpha- and 6beta-hydrogens of lathosterol.the fate of 6alpha- and 6beta-hydrogens of lathosterol during the transformation into 20-hydroxyecdysone was chased by feeding [3alpha,6beta-2h2]- and [3alpha,6alpha-2h2]-lathosterols to hairy roots of ajuga reptans var. atropurpurea. the behavior of 6beta-hydrogen, which mostly migrated to the c-5 position of 20-hydroxyecdysone, was in agreement with that of c-6 hydrogen of cholesterol. the results strongly supported the view that cholesterol and lathosterol are first metabolized into 7-dehydro ...199910658597
mechanism of c-2 hydroxylation during the biosynthesis of 20-hydroxyecdysone in ajuga hairy roots.feeding synthetic [2beta-2h]- and [2alpha-2h]-cholesterols to the hairy roots of ajuga reptans var. atropurpurea and 2h-nmr analysis of the biosynthesized 20-hydroxyecdysone revealed that hydroxylation at c-2 proceeds with retention of configuration. feeding [2alpha,3alpha-2h2]cholesterol followed by 2h-nmr analysis of the 2,3,22-triacetate of the resulting 20-hydroxyecdysone ruled out a mechanism which involves a partial loss of the 2alpha-hydrogen. the steric course of c-2 hydroxylation in aju ...200010726854
allocation of raffinose family oligosaccharides to transport and storage pools in ajuga reptans: the roles of two distinct galactinol synthases.raffinose family oligosaccharides (rfos) are important phloem transport and storage carbohydrates for many plants. ajuga reptans, a frost-hardy evergreen labiate, ideally combines these two physiological roles and served as our model plant to study the regulation and importance of rfo metabolism. galactinol is the galactosyl donor for the synthesis of raffinose (rfo-trisaccharide) and stachyose (rfo-tetrasaccharide), and its synthesis by galactinol synthase (gols) is the first committed step of ...200010758476
biosynthesis of sterols and ecdysteroids in ajuga hairy roots.hairy roots of ajuga reptans var. atropurpurea produce clerosterol, 22-dehydroclerosterol, and cholesterol as sterol constituents, and 20-hydroxyecdysone, cyasterone, isocyasterone, and 29-norcyasterone as ecdysteroid constituents. to better understand the biosynthesis of these steroidal compounds, we carried out feeding studies of variously 2h- and 13c-labeled sterol substrates with ajuga hairy roots. in this article, we review our studies in this field. feeding of labeled desmosterols, 24-meth ...200010783005
biosynthesis of 20-hydroxyecdysone in ajuga hairy roots: the possibility of 7-ene introduction at a late stage.administration of [3alpha-2h]-3beta-hydroxy-5beta-cholestan-6-one to hairy roots of ajuga reptans var. atropurpurea followed by 2h-nmr spectroscopic analysis of the resulting 20-hydroxyecdysone so formed revealed that the substrate was efficiently incorporated into the latter. additionally, [5beta,7alpha,7beta-2h3]-2beta,3beta-dihydroxy-+ ++5beta-cholestan-6-one was converted into 20-hydroxyecdysone. these findings clearly indicate that ajuga hairy roots are capable of introducing a double bond ...200010783979
[studies on the biosynthesis of sterol side chain in higher plants].campesterol (3) and dihydrobrassicasterol (4), typical c28-sterols in higher plants, are biosynthesized from a steroidal 24-ene precursor (desmosterol 1) via 24-methylenecholesterol (2) and 24-methyldesmosterol (5). a typical plant c29-sterol, sitosterol (6), is produced from 24-methylenecholesterol via isofucosterol (7) and 24-ethyldesmosterol (8). the biosynthetic mechanism, focussing stereochemical features, of these side-chain transformations has been studied in detail by feeding regio- and ...200011082699
ultrastructure of minor-vein phloem and assimilate export in summer and winter leaves of the symplasmically loading evergreens ajuga reptans l., aucuba japonica thunb., and hedera helix l.minor-vein ultrastructure and sugar export were studied in mature summer and winter leaves of the three broadleaf-evergreen species ajuga reptans var. artropurpurescens l., aucuba japonica thunb. and hedera helix l. to assess temperature effects on phloem loading. leaves of the perennial herb ajuga exported substantial amounts of assimilates in form of raffinose-family oligosaccharides (rfos). its minor-vein companion cells represent typical intermediary cells (ics), with numerous small vacuoles ...200111216844
mikimopine synthase (mis) gene on pri1724.by determination of the nucleotide sequence adjacent to the right border of t-dna of the mikimopine-type ri plasmid (pri1724) in agrobacterium rhizogenes, a new open reading frame (orf) encoding 318 amino acids was found. a transcript of 1.35 kb derived from this orf was observed in hairy roots of ajuga reptans by northern blotting analysis. including its own promoter and terminator, this orf was isolated from the pri1724 t-dna and introduced into tobacco plants by the agrobacterium-binary vecto ...200111223242
8-o-acetylharpagide is not an ecdysteroid agonist.we have reinvestigated the activity of 8-o-acetylharpagide, an iridoid glucoside, as an ecdysteroid agonist. elbrecht et al. (insect biochem. mol. biol. 26 (1996) 519) isolated a preparation of this compound from ajuga reptans l. and ascribed ecdysteroid agonist activity on the basis of the induction of an ecdysteroid-like response in drosophila melanogaster kco cells, the displacement of [3h]ponasterone a from the drosophila receptor and the activation of an ecdysteroid-regulated gene in a tran ...200111520686
characterization of transcription of genes involved in hairy root induction on pri1724 core-t-dna in two ajuga reptans hairy root lines.the detailed status of the transcription of genes on pril724 t-dna in two independent hairy root lines of the plant ajuga reptans, ar-4 and ar-24, which have several different characteristics, was obtained by northern blotting and a reverse transcription-polymerase chain reaction (rt-pcr) analysis. in the northern blotting analysis dna fragments corresponding to the putative open reading frames (orfs) as probes, transcripts from putative orfs 10 (1724rola), 11 (1724rolb), 12 (1724rolc), 13a and ...199811543203
acylated anthocyanidin 3-sophoroside-5-glucosides from ajuga reptans flowers and the corresponding cell cultures.four anthocyanins from ajuga reptans flowers and its cell cultures were isolated, and a fifth was also characterized by hplc-mass spectrometry. by means of chemical and spectroscopic analyses, their structures were identified as delphinidin 3-(p-coumaroyl-feruloyl)sophoroside-5-malonylglucoside, delphinidin 3-(diferuloyl)sophoroside-5-malonylglucoside, and cyanidin 3-(di-p-coumaroyl)sophoroside-5-glucoside, respectively. the other two were tentatively identified as delphinidin 3-(diferuloyl)soph ...200111557083
ajugatansins, neo-clerodane diterpenes from ajuga reptans.new neo-clerodane diterpenes have been isolated from ajuga reptans, and named ajugatansins, along with the previously reported ajugavensin a and ajugareptansone a. the structures of all the compounds were elucidated by spectroscopic means and comparison with closely related compounds reported in the literature. the new 6 alpha,19-diacetoxy-4 alpha,18-epoxy-1 beta-hydroxy-3 beta-(2-methyl-butanoyloxy)-neo-clerod-13-en-15,16-olide (ajugatansin a1) and 6 alpha,19-diacetoxy-4 alpha,18-epoxy-3 beta-h ...200111704966
purification and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan : galactan galactosyltransferase (ggt), from ajuga reptans leaves.galactan: galactan galactosyltransferase (ggt), an enzyme involved in the biosynthesis of the long-chain raffinose family of oligosaccharides (rfos) in ajuga reptans, catalyses the transfer of an alpha-galactosyl residue from one molecule of rfo to another one resulting in the next higher rfo oligomer. this novel galactinol (alpha-galactosyl-myo-inositol)-independent alpha-galactosyltransferase is responsible for the accumulation of long-chain rfos in vivo. warm treatment (20 degrees c) of excis ...200212060258
metabolism of the raffinose family oligosaccharides in leaves of ajuga reptans l. (inter- and intracellular compartmentation).we recently suggested that leaves of the frost-hardy species ajuga reptans l. (lamiaceace) contain two pools of raffinose family oligosaccharides (rfo): a large long-term storage pool in the mesophyll, possibly also involved in frost resistance, and a transport pool in the phloem (m. bachmann, p. matile, f. keller [1994] plant physiol 105: 1335-1345). in the present study, the inter- and intracellular compartmentation of anabolic rfo metabolism was investigated by comparing whole-leaf tissue wit ...199512228647
metabolism of the raffinose family oligosaccharides in leaves of ajuga reptans l. (cold acclimation, translocation, and sink to source transition: discovery of chain elongation enzyme).ajuga reptans is a frost-hardy, perennial labiate that is known for its high content of raffinose family oligosaccharide(s) (rfo). seasonal variations in soluble nonstructural carbohydrate levels in above-ground parts of ajuga showed that the rfo were by far the most predominant components throughout the whole year. rfo were lowest in summer (75 mg/g fresh weight) and highest in fall/winter (200 mg/g fresh weight), whereas sucrose and starch were only minor components. cold treatment (14 d at 10 ...199412232288
cloning, functional expression, and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosyltransferase, from common bugle leaves.galactan:galactan galactosyltransferase (ggt) is a unique enzyme of the raffinose family oligosaccharide (rfo) biosynthetic pathway. it catalyzes the chain elongation of rfos without using galactinol (alpha-galactosyl-myoinositol) by simply transferring a terminal alpha-galactosyl residue from one rfo molecule to another one. here, we report the cloning and functional expression of a cdna encoding ggt from leaves of the common bugle (ajuga reptans), a winter-hardy long-chain rfo-storing lamiacea ...200415034167
[ecdysone 20-monooxygenase activity of cytochrome p450 in plants and cell culture of ajuga reptans l].the concentration of cytochrome p450 and ecdysone 20-monooxygenase activity in plants and callus cell culture of the bugleweed ajuga reptans l. were determined. the maximal ecdysone 20-monooxygenase activity of cytochrome p450 was found in vegetative rosettes of intact plants. during the stage of flowering, the ecdysone 20-monooxygenase activity of cytochrome p450 in plant leaves was higher than in other organs. it was demonstrated that the content of ecdysteroids in callus cell culture is highe ...200415125192
kindler syndrome in native americans from panama: report of 26 cases.to investigate the clinical, genetic, and laboratory features of 26 patients with kindler syndrome.200415313809
a novel miniaturized flame ionization detector for portable gas chromatography.a novel miniaturized flame ionization detector (fid) operated by battery, which can be used as a detector in portable gas chromatography (gc) is devised and manufactured. it is characterized by the structure of building blocks, small volume, low energy consumption, and needing only two gases, which can be used for detection of hydrocarbons in portable gc. the miniaturized detector mainly includes a porous metallic diffuser plate, bugle-figuration collector, quartz capillary flame tip, and self-h ...200516176647
characterisation of an alpha-galactosidase with potential relevance to ripening related texture changes.alpha-galactosidase (ec 3.2.1.22) from ripe papaya (carica papaya l.) fruit was fractionated by a combination of ion exchange and gel filtration chromatography into three forms, viz., alpha-galactosidase 1, 2 and 3. the predominant isoform, alpha-gal 2, was probably a tetramer with a native molecular mass of about 170 kda and 52 kda-sized subunits and an estimated pi of 7.3. the subunit's n-terminal amino acid sequence shared high identity (97%) with the deduced sequence of a papaya cdna clone e ...200616325871
the c-terminal sequence from common bugle leaf galactan:galactan galactosyltransferase is a non-sequence-specific vacuolar sorting determinant.the ajuga reptans l. galactan:galactan galactosyltransferase (arggt) is a vacuolar enzyme that synthesizes long-chain raffinose family oligosaccharides (rfos), the major storage carbohydrates of this plant. arggt is structurally and functionally related to acid plant alpha-galactosidases (alpha-gals) of the glycosylhydrolase family 27, present in the apoplast or the vacuole. sequence comparison of acid alpha-gals with arggt revealed that they all contain an n-terminal signal sequence and a highl ...200717434166
molecular mechanisms underlying wound healing and anti-inflammatory properties of naturally occurring biotechnologically produced phenylpropanoid glycosides.two phenylpropanoid glycosides, verbascoside (vb) and teupolioside (tp), produced biotechnologically by syringa vulgaris and ajuga reptans plant cell cultures, were studied in vitro and in vivo for their anti-inflammatory and wound healing activities. it was shown that tp- and vb-containing extracts significantly accelerated wound healing and possessed remarkable anti-inflammatory action in the excision wound model. these effects correlated with the inhibition of reactive oxygen species release ...200717543237
tattoos defined.tattoo definitions from general, foreign language, medical dictionaries and textbooks are reviewed. in addition to the common usage "to mark the skin with pigments," the word tattoo, used as a noun, first meant a signal on a drum or bugle to call military men to quarters. this chapter includes a variety of colorful, cultural and historical references. the increasing popularity of tattoos stimulated the american academy of dermatology to produce the 2004 brochure tattoos, body piercing and other ...200717697927
teupolioside, a phenylpropanoid glycosides of ajuga reptans, biotechnologically produced by irbn22 plant cell line, exerts beneficial effects on a rodent model of colitis.the aim of the present study was to examine the effects of phenylpropanoid glycoside, teupolioside, biotechnologically produced by irbn22 ajuga reptans cell line, in rats subjected to experimental colitis. colitis was induced in rats by intracolonic instillation of dinitrobenzene sulfonic acid (dnbs). teupolioside was administered daily orally (0.2 or 2mgkg(-1)). on day 4, animals were sacrificed and tissues were taken for histological and biochemical analysis. four days after dnbs administratio ...200919070602
efficacy of a new jet nozzle for removal of carious dentin with an air abrasion system.a new version of an air abrasion device, which expelled abrasive sodium bicarbonate powder, was fabricated to remove carious tissue. this device had a motor-driven mechanism to control the amount of powder expelled. the purpose of this study was to estimate the abrading efficacy of this device, in particular the new jet nozzle, for removal of carious dentin. powder was supplied to the handpiece by a rotary gear attached to the powder control motor. two types of jet nozzles, a cylinder-type and a ...200819241693
c-29 ecdysteroids from ajuga reptans var. reptans.investigation of the ecdysteroid constituents of the herb ajuga reptans var. reptans resulted in the isolation of three new ecdysteroids, named reptanslactone a (2), reptanslactone b (3), and sendreisterone (5), and the known 24-dehydroprecyasterone (1) and breviflorasterone (4). the structures of compounds 1-5 were determined by spectroscopic methods including one- and two-dimensional nmr measurements.200919338317
[imaging study of lumbar intervertebral disc herniation and asymptomatic lumbar intervertebral disc herniation].using regional assignment to forked method to study lumbar intervertebral disc hemiation (bugle, hernia, prolapse) dependablity and reason of lumbar intervertebral disc herniation and asymptomatic lumbar intervertebral disc herniation.200919408758
frost tolerance in excised leaves of the common bugle (ajuga reptans l.) correlates positively with the concentrations of raffinose family oligosaccharides (rfos).mass increases in raffinose family oligosaccharides (rfos, alpha1,6-galactosyl extensions of sucrose) are well documented in the generative tissues of many plants upon cold acclimation, and they (i.e. mainly the two shortest rfo members, raffinose and stachyose) have been suggested as frost stress protectants. our focus here was on the longer rfo members as they commonly occur in the frost-hardy evergreen labiate ajuga reptans in its natural habitat, and accumulate to their highest concentration ...200919422612
raffinose in chloroplasts is synthesized in the cytosol and transported across the chloroplast envelope.in chloroplasts, several water-soluble carbohydrates have been suggested to act as stress protectants. the trisaccharide raffinose (alpha-1,6-galactosyl sucrose) is such a carbohydrate but has received little attention. we here demonstrate by compartmentation analysis of leaf mesophyll protoplasts that raffinose is clearly (to about 20%) present in chloroplasts of cold-treated common bugle (ajuga reptans l.), spinach (spinacia oleracea l.) and arabidopsis [arabidopsis thaliana (l.) heynh.] plant ...200919880397
boogie woogie bugle boy. 200920041620
committed differentiation of hair follicle bulge cells into sebocytes: an in vitro study.several studies have shown that hair follicle bugle cells can differentiate into hair follicles and contribute to the formation of the epidermis and sebaceous gland. although many lines of evidence have suggested that the renewal and maintenance of the sebaceous gland depends on hair follicle bulge cells, direct evidence supporting the in vitro differentiation of follicle bulge cells into sebaceous gland cells has not been found.201020465636
vasoactive effects of different fractions from two panamanians plants used in amerindian traditional medicine.cecropia obtusifolia (cecropiaceae) and psychotria poeppigiana (synonym: cephaelis elata, rubiaceae) are two latin american plants broadly used in traditional amerindian medicine. the former, together with many other species of the genus cecropia, share the folk reputation of curing heart failure, cough, asthma and bronchitis. the latter is used in panama by kuna and ngäbe buglé (guaymies) native indians for the treatment of dyspnea.201020600752
a cervid vocal fold model suggests greater glottal efficiency in calling at high frequencies.male rocky mountain elk (cervus elaphus nelsoni) produce loud and high fundamental frequency bugles during the mating season, in contrast to the male european red deer (cervus elaphus scoticus) who produces loud and low fundamental frequency roaring calls. a critical step in understanding vocal communication is to relate sound complexity to anatomy and physiology in a causal manner. experimentation at the sound source, often difficult in vivo in mammals, is simulated here by a finite element mod ...201020808882
the role of premorbid expertise on object identification in a patient with category-specific visual agnosia.the role of premorbid expertise in object identification was investigated in the category-specific visual agnosia patient elm. for several years prior to his stroke elm played bugle in a military band. we surmised that band membership would lead to preferential exposure to, and expertise for, brass instruments relative to other musical-instrument families. to test this hypothesis we assessed elm's musical instrument identification capability for brass and stringed instruments. testing was conduc ...200220957546
an investigation of musical performance anxiety in the marching arts.the purpose of this study was to investigate the prevalence of musical performance anxiety (mpa) in marching artists. the marching arts include high school and college marching bands, drum and bugle corps, and indoor color guard and percussion ensembles. drawing on a sample of 780 world class drum and bugle corps performers, we examined the prevalence of somatic and cognitive symptoms of mpa. we also examined differences in endorsement of symptoms by performing section (i.e., brass players, perc ...201121442134
biological activity of natural phytoecdysteroids from ajuga iva against the sweetpotato whitefly bemisia tabaci and the persea mite oligonychus perseae.background: ecdysteroids are steroid hormones that control moulting and govern several changes during metamorphoses in arthropods. the discovery of the same molecules (phytoecdysteroids) in several plant species displayed a wide array of rather beneficial agricultural impact. many representatives of the genus ajuga plants contain phytoecdysteroids with a 5β-7-ene-6-one system exhibiting physiological activities in insects. results: by means of chromatographic (silica gel column, tlc) and lc-ms, ...201121604353
four new iridoid glucosides from ajuga reptans.four new iridoid glucosides were isolated from the whole plant of ajuga reptans l. (labiatae) along with four known iridoid glucosides, one known diterpenoid glycoside, one known aliphatic alcohol glycoside, and three known ecdysteroids. their chemical structures were determined on the basis of spectroscopic data and chemical evidence. the diterpenoid glycoside exhibited 1,1-diphenyl-2-picrylhydrazyl (dpph) radical and h(2)o(2) scavenging activities.201121804257
Big Five personality traits and performance anxiety in relation to marching arts satisfaction.To examine the Big Five personality traits and performance anxiety in relation to marching arts satisfaction. Participants: Data were collected from 278 instrumentalists (i.e., brass players and percussionists) and color guard performers (e.g., dancers) representing six world class drum and bugle corps.201122045536
antimalarial natural products drug discovery in panama.context: malaria is still a major public health problem. the biodiversity of the tropics is extremely rich and represents an invaluable source of novel bioactive molecules. for screening of this diversity more sensitive and economical in vitro methods are needed, flora of panama has been studied based on ethnomedical uses for discovering antimalarial compounds. objective: this review aims to provide an overview of in vitro screening methodologies for antimalarial drug discovery and to present ...201222196582
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