| molecular evolution of glycogen phosphorylase and aminotransferases of vertebrate muscle tissue. | comparative biochemical studies on phosphorylase b, aspartate aminotransferase and alanine aminotransferase in muscles of various vertebrates (the lamprey lampetra fluviatilis, dogfish squalus acanthias, rays dasyatis pastinaca and raja clavata, teleosts scorpaena porcus, spicara smaris, esox lucius, tinca tinca, abramis brama, lucioperca lucioperca, cyprinus carpio, salmo ischchan, frog rana temporaria, tortoise testudo horsfieldi) revealed some peculiarties of their molecular evolution. it was ... | 1977 | 17251 |
| trout mortalities as a result of streptococcus infection. | excessive mortalities were experienced in the bigger rainbow trout (salmo gairdneri) at an efficiently managed trout farm. all tests for known toxins in the feed and water proved to be negative. a faecal streptococcus which belongs to the lancefield group d but which could not be identified as belonging to any of the recognized species within this group, was isolated from the spleens, livers and kidneys of affected fish. pathogenicity studies with this organism proved it to be highly fatal to tr ... | 1979 | 121769 |
| phylogeny of lymphocyte heterogeneity. i. membrane immunoglobulins of teleost lymphocytes. | immunofluorescence studies of bream lymphoid tissues revealed that our 90% of the lymphocytes from blood, anterior kidney, spleen and thymus exhibited membrane immunoglobulin determinants. furthermore, a majority of such cells were observed to undergo patching and capping when the membrane proteins were complexed with antisera to fish serum immunoglobulins. lactoperoxidase catalysed radioiodination, detergent lysis and immunoprecipitation with appropriate antisera were employed to study the prop ... | 1977 | 346400 |
| localization of alkaline and acid phosphatase activity in the intestine of healthy breams (abramis brama l.) and those infected with plerocercoid of tapeworm ligula intestinalis (linné 1758). | the examination included 40 breams 4 to 7 years old (18 infected and 22 uninfected). alkaline and acid phosphatase activity, as shown by the azo-coupling method, has been localized in the oesophagus and the intestine, being the strongest in the epithelium. it was distinctly less intensive in the lamina propria mucosae and in the intermuscular connective tissue of the oesophagus, in the submucosa, in the cells of auerbach plexus and in the blood vessel walls. besides only the acid phosphatase act ... | 1977 | 409038 |
| the haemocytology and histology of the haemopoietic organs of south african fresh water fish. i. the haemopoietic organs of clarias gariepinus and sarotherodon mossambicus. | the haemopoietic organs of the catfish, clarias gariepinus, and the mozambique bream, sarotherodon mossambicus, were studied. in both species the primary haemopoietic organs are the pronephros, the mesonephros and the spleen. the peritoneum and, particularly in catfish, the omentum are of secondary importance in haemopoiesis. | 1979 | 551371 |
| calcification in an ageing ligula intestinalis (l) plerocercoid from a bream (abramis brama l.). | light and transmission electron microscopy of the strobila of a large old ligula intestinalis plerocercoid has revealed microcrystals with a morphology similar to that of microapatite crystals from vertebrates. analysis of the microcrystals with emma-4 showed them to contain calcium and phosphorus. the tissue in the immediate vicinity of the microcrystals shows signs of necrosis while further away it is histologically normal. | 1977 | 919699 |
| seasonal variation in the population dynamics of caryophyllaeus laticeps. | the biological parameters which generate seasonal fluctuations in the size of populations of caryophyllaeus laticeps are examined in detail. a mathematical model is formulated to describe the dynamics of the adult parasite within the fish host abramis brama, and the predictions of the model are compared with observed population data. the seasonality of parasite population size is shown to be caused by the combined effects of a temperature dependent mortality rate and flucate of larval parasites. ... | 1976 | 967520 |
| [alkaline and acid phosphatases and hemosiderin in the internal organs of bream (abramis brama l.) infected with plerocercoid ligula intestinalis (linne, 1758)]. | | 1975 | 1146335 |
| the use of carp, cyprinus carpio (linnaeus, 1758), instead of rabbit as antibody donor in immunological research on fishes. | antisera produced by carp were tested in the differentiation between two cyprinid fishes, bream and roach, and between the lipovitellins of two grey mullets, mugil cephalus and liza ramada. these antisera were more specific in the recognition of different fish species than those produced by rabbits. an antiserum against carp lipovitellin was produced with male carp as antibody donor. | 1975 | 1211668 |
| [a study of ratios between histone fractions]. | the amount of individual fractions in the whole histone isolated from the blood of hen, frog (rana ridibunda), bream (abramis brama), from rat thymus and from the locust (schistocerca gregaris) was studied quantitatively. it is shown that the ratios between fractions f2a1, f2b, f2a2 and f3 are fixed. the share of each fraction in the sum of fractions f2a1, f2b, f2a2, f3 was found to be approximately 22.5, 30.5, 21.0 and 26.0 per cent, respectively. the share of the fraction f1 can variate within ... | 1975 | 1214808 |
| [fish parasites as bioindicators of the pollution of bodies of water]. | the paper presents the results of studies on the effect of wastes of the cherepovets iron-and-steel works containing phenol, naphthaline and oil products on fish parasites of the sheksna part of the rybinsk water reservoir (the volga river system). it has been shown that the number of highly sensitive ectoparasites of abramis brama (protozoa, dactylogyrus monogeneans, ergasilus sieboldi crustaceans and caspiobdella fadejewi leeches) considerably decreases in the zone of pollution. it has been fi ... | 1992 | 1299804 |
| immunocytochemistry of somatotrophs, gonadotrophs, prolactin and adrenocorticotropin cells in larval sea bream (sparus auratus) pituitaries. | the chronological appearance of endocrine cells in the pituitary of sea-bream (sparus auratus) larvae was studied using antisera against salmon prolactin, trout growth hormone, salmon gonadotropin and n-terminal human adrenocorticotropin. the larval pituitary (1-12 days after hatching) was oval in shape and was composed of a dense mass of cells with few neurohypophysial fibres. by 60 days after hatching it began to resemble the adult and was divisible into a distinct rostral pars distalis contai ... | 1992 | 1330314 |
| metabolism of sodium nifurstyrenate, a veterinary antimicrobial nitrofuran, in animals and fish. | sodium nifursyrenate [beta-(5-nitro-2-furyl)-p-carboxystyrene sodium salt, nsa-na] is an antibacterial nitrofuran which has been widely used for prevention and treatment of bacterial infections in fish in japan. when nsa-na was anaerobically incubated with rabbit liver cytosol and 2-hydroxypyrimidine, 1-(p-carboxyphenyl)-5-cyano-3-oxo-1,4-pentadiene (cyano-pentadienone), 1-(p-carboxyphenyl)-5-cyano-3-oxo-1-pentanone (cyano-pentanone), and 1-(p-carboxyphenyl)-5-cyano-3-pentanone (cyano-pentanone) ... | 1992 | 1352214 |
| attenuation of sound by schooling fish. | attenuation of sound by schooling fish was measured or estimated for yellowtail (seriola quinqueradiata), sea bream (pagrus major), and spotted mackerel (scomber australasicus) in culture nets and a net cage. the results, along with those of other researchers, are normalized to obtain extinction cross sections per 2/3 power of the body weight. in the normalized cross sections there are no large differences among fish species, sizes, and measuring methods. the attenuation increases slightly with ... | 1992 | 1506535 |
| cortisol content of eggs and larvae of teleosts. | the whole-animal content of the cortisol was measured in embryos and larvae of tilapia (oreochromis mossambicus), rainbow trout (oncorhynchus mykiss), ayu (plecoglossus altivelis), milkfish (chanos chanos), and yellowfin bream (acanthropagrus latus) by radioimmunoassay following the validation of an extraction method. the total cortisol content in tilapia was 50.3 +/- 19.1 pg immediately following fertilization, then decreased abruptly and maintained a lower level of 10-17 pg until larval hatchi ... | 1992 | 1601267 |
| observations on the pigmentation of the monogeneans, anoplodiscus spp. (family anoplodiscidae) in different microhabitats on their sparid teleost hosts. | the monogeneans anoplodiscus australis and a. cirrusspiralis infect the fins of their respective hosts, the yellowfin bream, acanthopagrus australis, and the snapper, pagrus auratus. in these habitats the adult parasite contains melanin-like pigment deposits within the body. very young parasites lack the pigment but deposits of pigment appear and coalesce into dense deposits within the parasite as it grows. the evidence suggests that the pigment is synthesized within the body of the parasite rat ... | 1992 | 1644520 |
| seasonal changes of superoxide dismutase activity in erythrocytes of abramis brama from two different types of poland lakes. | | 1991 | 1786462 |
| [the hydrolysis and transport of sugars in the cestode caryophyllaeus laticeps and in the intestines of its host, the bream]. | a comparative study of hydrolysis and transport of carbohydrates by the cestode c. laticeps and in the intestine of its host, bream, has revealed a considerable influence of the host's enzymes on the characteristics of the parasite's enzyme system. the cestodes have been found to possess effective transport systems of glucose absorbing it in an active way while in the intestine of bream during the glucose absorption prevail the mechanisms of simple and easy diffusion. | 1991 | 1808595 |
| amino acid sequence of growth hormone isolated from medium of incubated pituitary glands of tilapia (oreochromis mossambicus). | the amino acid sequence of tilapia (oreochromis mossambicus) growth hormone (gh) was determined directly by edman degradation of peptide fragments generated by lysyl endopeptidase and trypsin digestion. the n-terminal residue was deduced to be pyroglutamic acid through the use of pyroglutamyl aminopeptidase; its removal allowed amino acid sequence determination of the remainder of the n-terminal trypsin peptide by edman degradation. tilapia gh is composed of 187 amino acid residues and shows hig ... | 1991 | 2019405 |
| presence of specific growth hormone binding sites in rainbow trout (oncorhynchus mykiss) tissues: characterization of the hepatic receptor. | the present work outlines the presence of specific binding for chinook salmon growth hormone (sgh) in different tissue preparations of rainbow trout. optimal incubation conditions (ph, tris, mgcl2) were determined. specific binding was very sensitive to salt concentration during incubation. the specific binding reached a plateau after 15 and 25 hr of incubation at 12 and 4 degrees. at 20 degrees, specific and nonspecific binding were not stable. specific binding dissociation was slower than asso ... | 1991 | 2026319 |
| morphology and ultrastructure of cryptobia eilatica n. sp. (bodonidae: kinetoplastida), an ectoparasite from the gills of marine fish. | a marine kinetoplastid flagellate, cryptobia eilatica n. sp., is described from the gills of cultured gilt-head sea bream sparus aurata l. and wild black-spot sea bream diplodus noct (valenciennes) in the red sea. the trophozoite is elongated and lacks a contractile vacuole and undulating membrane. the body averages 13.5 x 4.1 microns, anterior flagellum 9.7 microns and free portion of recurrent flagellum 15.2 microns. the ultrastructural features of the species exhibit great similarity to vario ... | 1990 | 2086780 |
| fructose 2,6-bisphosphate in liver of sparus aurata: influence of nutritional state. | 1. fructose 2,6-bisphosphate (fru-2,6-p2) has been measured in liver and muscle of gilthead sea bream fish, sparus aurata. 2. the fru-2,6-p2 levels in liver depend on the diet given to the fish: in fish fed a high carbohydrate diet, the fru-2,6-p2 levels are higher than any one previously reported. these changes are associated with differences in the phosphofructokinase 2 activity. 3. fru-2,6-p2 levels has also been measured in liver of sparus aurata after different fasting periods. in starved f ... | 1990 | 2142067 |
| nucleotide sequence of cdna and primary structure for hard tail growth hormone. | full-length cdna for hard tail growth hormone (htgh) has been cloned, and the nucleotide and deduced amino acid sequences have been analyzed. htgh is composed of 188 amino acid residues, and it shows 79, 74, 72, 59, 56, 37, 33 and 30% identity of amino acid with yellow tail, tuna, sea bream, flounder, salmon, blue shark, bullfrog and human ghs, respectively. | 1990 | 2223886 |
| [the spatial structure and interrelation of plerocercoids of digramma interrupta (cestoda, ligulidae) and the bream (abramis brama) in the kuibyshev reservoir]. | the character of the distribution of the plerocercoid d. interrupta in the bream abramis brama from the kuibyshev water reservoir was studied. the spread of the parasites in a. brama population is of complex character and changes with the increase of fish body length. the factors affecting the maintenance and regulation of the relationships in the host-parasite system (size composition of fishes, peculiarities of the host's genotype, values of occurrence of different numbers of the parasites, va ... | 1989 | 2530489 |
| histochemistry of glycoconjugates in the gallbladder epithelium of ten animal species. | a battery of seven lectins and several conventional mucin histochemical techniques were used to identify the epithelial mucins of the gallbladder of ten species: man, rabbit (oryctolagus cuniculus, mammalia), hamster (mesocricetus auratus, mammalia), chicken (gallus gallus, bird), sparrow (passer domesticus, bird), moorish gecko (tarentola mauritanica, reptilia), ladder snake (elaphe scalaris, reptilia), lake frog (rana perezi, amphibia), natterjack toad (bufo calamita, amphibia) and gilthead se ... | 1989 | 2722572 |
| natural environment surpasses polluted environment in inducing dna damage in fish. | measurement of specific dna adduct concentrations in target tissues of organisms may provide a key biologic end-point of exposure to environmental carcinogens. using a general and highly sensitive assay with 32-p-postlabeling, we found that natural populations of freshwater fish species chub, barbel, bream and carp, as well as a marine fish mugil, revealed the presence of four to nine qualitatively similar adducts irrespective of whether they were caught from unpolluted or polluted waters. no st ... | 1989 | 2736723 |
| [the biochemical effects of plerocercoids of the tapeworm ligula intestinalis (l.) on the bream abramis brama (l.)]. | | 1988 | 3388862 |
| [the relation between water temperature and intensity of infection of the bream with parasites of the genus dactylogyrus]. | | 1988 | 3388865 |
| [the occurrence of parasites in the bream (abramis brama l.), roach (rutilus rutilus l.) and perch (stizostedion lucioperca l.) in the włocławek dam reservoir on the vistula river]. | | 1988 | 3388868 |
| [intestinal arginase in the phylogenetic development of animals]. | the activity of intestinal arginase was studied in 31 species belonging to the types annelides, arthropoda and chordata. large variations of the enzyme activity were tested. the differences were of 2 orders of magnitude--100 times. four groups were selected: animals with very low arginase activity up to 0.20 microm of ornithine/mg of protein/15 min; this group comprises both phylogenetically lower and higher animals (cockroach, locust, frog, duck, domestic hen, broiler chicken, horse, hyppopotam ... | 1987 | 3452799 |
| fusarium oxysporum in red sea bream (pagrus sp.). | | 1986 | 3503145 |
| treatment of fish parasites. 2. effects of praziquantel, niclosamide, levamisole-hcl, and metrifonate on monogenea (gyrodactylus aculeati, diplozoon paradoxum). | in research for successful treatment of fish parasitized by monogeneans, four substances (praziquantel, niclosamide, levamisole-hcl, and metrifonate) were tested in vivo against gyrodactylus aculeati parasitizing the skin of sticklebacks (gasterosteus aculeatus). fish were incubated in water containing different solutions of the drugs. praziquantel, niclosamide and levamisole-hcl were effective against gyrodactylus aculeati. praziquantel caused irreversible lesions in the parasite tegument (begi ... | 1987 | 3615395 |
| [metal concentrations in fishes of the rednitz river]. | we investigated 39 fish species (eel, brown trout, chub, carp bream, roach, perch, pike etc.) from the river rednitz (north bavaria) for content of the metals mercury, lead, cadmium, arsenic, selenium and zinc. the residue tolerance level for mercury (1 mg/kg) and the standard value of the federal public health office for lead (0.5 mg/kg) and cadmium (0.05 mg/kg) have not been exceeded in any case. because a small selenium concentration gives protection against the toxicity of mercury, we also h ... | 1985 | 4024745 |
| poor evidence for insulin/insulin-like immunoreactivity in the cns of lower vertebrates. | the localization of insulin/insulin-like immunoreactivity was studied in the brain and the pancreas of an amphibian (clawed frog [xenopus laevis daudin]) and in the cns of two teleost fishes (bream [abramis brama l.] and rudd [scardinius erythrophthalmus l.] using the indirect immunofluorescence technique. with exception of cerebellar purkinje cells no neuronal elements were found to contain insulin-like material. the frog pancreas was characterized by a strong immunofluorescence in cells produc ... | 1981 | 6795888 |
| the haemolytic plaque assay in carp (cyprinus carpio). | a haemolytic plaque assay for the enumeration of antibody forming cells in the carp (cyprinus carpio) is described. serum of bream (abramis brama) turned out to be a more reliable complement source than allogeneic carp serum. the addition of 3--5% bream serum to a mixture of immune lymphoid carp cells and xenogeneic erythrocytes gave optimal results. higher amounts of bream complement inhibited plaque formation. plaque formation was also suppressed when inactive or heat-inactivated bream or carp ... | 1980 | 6987315 |
| morphological analysis of the fish heart ventricle: myocardial and connective tissue architecture in teleost species. | light and scanning electron microscopy were used to study the structure of the heart ventricle in three species of marine teleost fishes: the hake (merluccius merluccius), the angler fish (lophius piscatorius) and the sea bream (pagellus centrodontus). our findings show the ventricle to be shaped differently in each species: tubular in the hake, saccular in the angler fish and pyramidal in the sea bream. from a structural viewpoint, interest was centered on two aspects: organization of the myoca ... | 1995 | 7541184 |
| biochemical responses and environmental contaminants in breams (abramis brama l.) caught in the river elbe. | adult breams (abrama brama l.) were caught in october 1992 at seven stations in the river elbe and at one nonpolluted reference site, the belauer see. the locations of the sampling stations extended from the city of steti (tschechien republic) to the city of hamburg. indices of biochemical effects in microsomal and cytosolic fractions of livers were studied by measuring cytochrome p450-dependent monooxygenases and glutathione-s-transferase (gst) activities. in addition, levels of mercury and 35 ... | 1995 | 7544264 |
| tissue-type transglutaminase from red sea bream (pagrus major). sequence analysis of the cdna and functional expression in escherichia coli. | a cdna clone encoding a tissue-type transglutaminase (tgase) was isolated from a cdna library prepared from the liver of red sea bream (pagrus major). the cdna sequence had an open reading frame coding for a protein of 695 amino acids and showed 43% identity to the sequence of guinea pig liver tgase, revealing a relatively low overall similarity. however, the 25-amino-acid sequence containing the putative active site (cys272) of the enzyme was completely conserved between the two species, and wa ... | 1995 | 7556189 |
| evidence for a direct action of gh on haemopoietic cells of a marine fish, the gilthead sea bream (sparus aurata). | receptors for gh were characterized in the head kidney of gilthead sea bream (sparus aurata), using radioiodinated and biotinylated ligands. the specific binding of radiolabelled recombinant gilthead sea bream gh (rsbgh) to head kidney membrane preparations was dependent on membrane concentration. salmon prolactin, salmon gonadotrophin and carp gonadotrophin did not compete for 125i-labelled rsbgh-binding sites. unlabelled rsbgh competitively displaced 125i-labelled rsbgh bound to head kidney me ... | 1995 | 7595141 |
| parasites of south african freshwater fish. vii. nematodes of some scaled fishes from the hartbeespoort dam, transvaal. | the nematode parasites of 16 large-scaled yellowfish, barbus marequensis, six silverfish, barbus mattozi, six small-scaled yellowfish, barbus polylepis, 52 canary kurper, chetia flaviventris, 11 carp, cyprinus carpio, 45 mozambique bream, oreochromis mossambicus and a single-banded bream, tilapia sparrmani, caught in the hartbeespoort dam, transvaal, were collected, identified and counted. contracaecum spp. larvae were recovered from one o. mossambicus, 40 chetia flaviventris, three cyprinus car ... | 1994 | 7596571 |
| cloning and characterisation of a fish aldolase b gene. | a full length cdna clone representing an aldolase mrna was isolated from a sea bream (sparus aurata) liver cdna library. sequencing of this clone revealed it to encode a 364 amino acid protein with 74% amino acid identity to human aldolase b and slightly lower similarity to human aldolase a and c. in view of the sequence data and of northern blot analysis showing strong expression of a 1.6 kb transcript in liver it was concluded that the cloned gene represents aldolase b. this clone represents t ... | 1995 | 7632737 |
| primary structure of solitary form of gonadotropin-releasing hormone (gnrh) in cichlid pituitary; three forms of gnrh in brain of cichlid and pumpkinseed fish. | gnrh is a decapeptide family with at least nine distinct structures. vertebrates, except for most placental mammals, have more than one of these gnrh forms within the brain. we report chromatographical and immunological evidence that three forms of gnrh are in the brains of both cichlid (haplochromis burtoni) and pumpkinseed (lepomis gibbosus) fishes. we argue that the three forms correspond to those previously described as sea bream gnrh (sbgnrh), chicken gnrh-ii and salmon gnrh. in contrast, o ... | 1995 | 7644702 |
| the use of recombinant gilthead sea bream (sparus aurata) growth hormone for radioiodination and standard preparation in radioimmunoassay. | a gilthead sea bream growth hormone (sbgh) obtained by cloning and expression of sbgh cdna was used to develop a sensitive and specific radioimmunoassay (ria). iodination of recombinant sbgh (rsbgh) was performed by the classical chloramine-t method. specific antiserum, raised in rabbits, was added in a final dilution of 1/36,000. the minimum detectable dose was 30 pg, and the midrange of the assay (ed50) was 275 pg. intra- and inter-assay coefficients of variation (cv) were 3.3 and 5.8% at ed50 ... | 1995 | 7669108 |
| natural hemolytic and bactericidal activities of sea bream sparus aurata serum are effected by the alternative complement pathway. | sea bream serum displayed bactericidal and hemolytic activities. these activities were depleted when serum was incubated with different activators of the alternative complement pathway (acp). ethylenediaminetetraacetic acid (edta) inhibited both the hemolytic and bactericidal activities, while ethyleneglycol-bis (b-aminoethyl ether)-n, n, n'-tetraacetic acid (egta) was not inhibitory. an antibody against the putative third component of sea bream component (c3) was produced. it was observed by im ... | 1995 | 7676614 |
| [parasitic protozoa of the bream (abramis brama l.) in the rybinsk reservoir]. | the fauna of parasitic protozoans of the bream (abramis brama) from different reachs of the rybinsk water reservoir was investigated. 350 fish specimens have been examined by the method of total dissection. the greater number of parasites and diversity (33 species) were observed in the volzhski reach: infusoria--16 species, myxosporidia 12, flagellata--4, dermocystidia--1. in the breams of sheksninski reach 18 species of parasites were recovered: infusoria--8, myxosporidia--8, flagellata--1, der ... | 1994 | 7700685 |
| purification and characterization of a tissue-type transglutaminase from red sea bream (pagrus major). | a tissue-type transglutaminase (tgase) was purified from liver tissue of the red sea bream, pagrus major, by ion-exchange chromatography and heparin-sepharose affinity chromatography. its activity was assessed using a fluorometric assay to measure the incorporation of monodansylcadaverine into n,n'-dimethyl casein. the molecular mass of purified tgase was estimated to be 78 kda by sds-polyacrylamide gel electrophoresis. the enzyme required ca2+ to express its activity, although 10 mm sr2+ also a ... | 1994 | 7765597 |
| survey of the paratenic hosts of anguillicola crassus in lake velence, hungary. | anguillicola crassus infection of paratenic hosts was investigated in lake velence, hungary, in october 1992. a total of 155 specimens of 8 species (gymnocephalus cernuus, lepomis gibbosus, stizostedion lucioperca, abramis brama, alburnus alburnus, cyprinus carpio, pseudorasbora parva and rutilus rutilus) were examined: all species proved to be infected. there were large differences in the prevalence of infection among fishes, but the prevalence was generally higher in perciform fishes and it wa ... | 1994 | 7810405 |
| [ambiphrya ameiuri (ciliophora: peritricha): its ultrastructure and distribution on the body of carp fry]. | the mass infection of larvae and youngsters of carp fishes with the peritrichoid ambiphrya ameiuri has been recorded in natural condition in the rybinsk water reservoir. the uneven localisation of ambiphyrans on the body surface has been observed on the hosts blicca bjorkna and abramis brama. most high density of parasites was on the breast, back and tail fins. the morphological variability of ambiphyrans in dependence upon their localisation on the fish body has been observed. details being sig ... | 1994 | 7824286 |
| parasites of south african freshwater fish. vi. nematode parasites of some fish species in the kruger national park. | the nematode parasites of 30 spot-tailed robbers, brycinus imberi, five tiger-fish, hydrocynus vittatus, 77 large-scaled yellowfish. barbus marequensis, two mudsuckers, labeo molybdinus, 114 catfish, clarias gariepinus, 46 silver barbel, schilbe intermedius, 66 squeakers, synodontis zambezensis, three eels, anguilla spp., 83 mozambique bream, oreochromis mossambicus, 81 red-breasted bream, tilapia rendalli swierstrae and 32 large-mouthed bream, serranochromis meridianus, caught in the sabie, cro ... | 1994 | 7898896 |
| ovarian melanotropic peptides and adaptation in two teleostean species: sparus aurata l. and dicentrarchus labrax l. | the ovarian tissue of dicentrarchus labrax and sparus aurata displays two immunoreactive peaks that correspond to the elution time of human des-acetyl alpha-msh [acth(1-13)-amide] and human alpha-msh. in view of the close identity between the primary structure of fish and human alpha-msh, these data demonstrate that two msh-related peptides are present both in sea bream and sea bass ovary. alpha-msh-like immunoreactivity was found within both granulosa and thecal layers of mature follicles, as w ... | 1994 | 7984515 |
| three forms of gonadotropin-releasing hormone characterized from brains of one species. | most vertebrate species have more than one form of gonadotropin-releasing hormone (gnrh) in their brains, but it is not clear whether each form has a distinct function. we report that sea bream (sparus aurata) brains have three forms of gnrh, one of which is described herein and is called sea bream gnrh (sbgnrh). the primary structures of two forms were determined by edman degradation and mass spectral analysis. the amino acid sequence of sbgnrh is pglu-his-trp-ser-tyr-gly-leu-ser-pro-gly-nh2. t ... | 1994 | 7991588 |
| insulin binding and receptor tyrosine kinase activity in skeletal muscle of carnivorous and omnivorous fish. | we characterized the insulin receptors in skeletal muscle from several fish species with different nutritional preferences: brown trout (salmo trutta fario), gilthead sea bream (sparus aurata), tilapia (tilapia mossambica), and carp (cyprinus carpio), semipurified by affinity chromatography (wheat germ agglutinin-agarose). total specific binding and number of receptors per unit weight of piscine white skeletal muscle were lower than those values found in mammalian skeletal muscle. the same param ... | 1994 | 8024051 |
| parathyroid hormone-related protein is a factor in normal fish pituitary. | using antibodies to the amino-terminal region of human parathyroid hormone-related protein (pthrp) we have demonstrated pthrp immunoreactivity in pituitaries and plasma of the sea bream (sparus aurata). pituitary cells at two distinct locations contained immunodetectable pthrp; an anterior group in the rostral pars distalis which also contained immunoreactive thyroid stimulating hormone (tsh), and a posterior group lying at the border of the pars intermedia and proximal pars distalis between cel ... | 1993 | 8282170 |
| adherence and invasive capacities of the fish pathogen pasteurella piscicida. | pasteurella piscicida strains were weakly or moderately adherent to cell lines, the levels of attachment being variable depending on the cells employed. all the isolates exhibited the highest binding capacity to chse-214 cells. adhesive capacities were affected by heat and sugars but not by proteinase k or by treatment with antisera raised against the lipopolysaccharides of p. piscicida, implicating components of glycoprotein(s) as ligands in the adhesion process. the isolates showed a great bin ... | 1996 | 8674968 |
| molecular forms of gnrh in three model fishes: rockfish, medaka and zebrafish. | three species of fish have become important in the study of reproduction and development. rockfish are a model for developmental studies of live-bearing perch-like fish, whereas medaka and zebrafish are models for developmental and genetic studies. the forms of gnrh are identified in the brains of each of these fish and in the pituitary of the rockfish to investigate the role of gnrh in reproduction. here, we report that grass rockfish (sebastes rastrelliger) have three forms of gnrh in brain ex ... | 1996 | 8708558 |
| immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish. | using antisera to regions of human parathyroid hormone-related protein (pthrp) the saccus vasculosus (sv) of the sea bream (sparus aurata) has been shown to contain immunoreactive pthrp. by immunohistochemistry (ihc) the epithelial coronet cells in fixed and wax-embedded sv tissue reacted with antisera to the prepro region of human pthrp (-13 to +2), the n-terminus pthrp (1-16), and the midmolecule pthrp (50-69). sodium dodecyl sulfate-polyacrylamide gel electrophoresis of saccus extracts and in ... | 1996 | 8713647 |
| ontogeny of growth hormone protein and mrna in the gilthead sea bream sparus aurata. | the ontogeny of growth hormone (gh) protein and mrna was studied in the gilthead sea bream sparus aurata by northern blot, reverse transcription-polymerase chain reaction (rt-pcr) and western blot analyses. total rna was prepared from a pool of larvae collected on different days after hatching. when hybridized to sparus aurata gh cdna, gh specific mrna was first seen on day 3 post-hatching. levels increased on subsequent days and reached the highest levels on day 9; thereafter a decrease was not ... | 1996 | 8717446 |
| crowding stress induces changes in serum haemolytic and agglutinating activity in the gilthead sea bream sparus aurata. | sea bream sparus aurata were subjected to crowding for 3 weeks and the levels of plasma cortisol and glucose were determined in order to ascertain the occurrence of stress. at the same time, selected indicators of the immune response were monitored through blood lymphocyte counting and selected humoral responses such as the haemagglutination activity (ha) of serum towards rabbit erythrocytes (rarbc) and the alternative complement pathway (acp) levels. the results show an initial moderate increas ... | 1996 | 8797287 |
| development of enzyme immunoassays for 3,5,3'-triiodo-l-thyronine and l-thyroxine: time-course studies on the effect of food deprivation on plasma thyroid hormones in two marine teleosts, sea bass (dicentrarchus labrax l.) and sea bream (sparus aurata l.). | the effects of short-term food deprivation and photoperiod on plasma thyroid hormone levels of sea bass and sea bream were studied. animals were acclimated under constant photoperiod regime (15l/9d) and feeding times (2 hr after light onset and 2 hr before light offset). time-course studies involved monitoring plasma hormone levels every 4 hr throughout 1.5 24-hr cycles. plasma 3,5, 3'-triiodo-l-thyronine (t3) and l-thyroxine (t4) were assayed using a newly developed competitive enzyme immunoass ... | 1996 | 8812399 |
| kinetics of adhesion of selected fish-pathogenic vibrio strains of skin mucus of gilt-head sea bream (sparus aurata l.). | the kinetics of adhesion of vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. a modified langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (vibrio alginolyticus dp1he4 and vibrio anguillarum-like dc12r8 and dc12r9) showed a saturation kinetics except for v. alginolyticus (can), which showed a proportional adsorption kinetics. the adhesive capability for skin mucus does not seem to be an essential viru ... | 1996 | 8837420 |
| a method for direct dna amplification of uncharacterized dna viruses and for development of a viral polymerase chain reaction assay: application to the red sea bream iridovirus. | a method is described for isolating a dna segment of a virus for which no protein or dna sequence information is available. this segment can then be used to develop a pcr-based assay for the virus. the method is based on the widespread presence and strong conservation of the ribonucleotide reductase gene among dna viruses. the validity of the procedure is demonstrated by development of an assay for the fish iridovirus. we report the direct isolation from infected fish of a 738-bp segment of the ... | 1996 | 8923958 |
| production of recombinant insulin-like growth factor-ii in the development of a radioimmunoassay in rainbow trout (oncorhynchus mykiss). | rainbow trout (oncorhynchus mykiss) insulin-like growth factor-ii (igf-ii) cdna was amplified by reverse transcription-polymerase chain reaction and cloned into an expression vector (parhs3) for production by escherichia coli. recombinant rainbow trout igf-ii (rtigf-ii) was recovered from bacterial inclusion bodies, solubilized, and purified by gel filtration chromatography under reducing conditions. after refolding of the peptide by dialysis at basic ph, we obtained a final yield of 820 microgr ... | 1996 | 8930606 |
| the capacity of liver microsomes to form benzo[a]pyrene-diolepoxide-dna adducts and induction of cytochrome p450 1a in feral fish exposed to pulp mill effluents. | an investigation was made of cytochrome p4501a (cyp1a) induction, determined by the activity of erod (7-ethoxyresorufin o-deethylase), and formation of benzo[a]pyrene-diolepoxide-dna (bpde-dna) adducts, measured by synchronous fluorescence spectrophotometry, in liver microsomes of perch (perca fluviatilis), bream (abramis brama), and roach (rutilus rutilus). fish were collected from the southern part of lake saimaa (finland), an area polluted by effluents from the pulp and paper industry. in add ... | 1996 | 8950535 |
| cloning and expression of somatolactin, a pituitary hormone related to growth hormone and prolactin from gilthead seabream, sparus aurata. | a pituitary hormone, somatolactin (sl), belonging to the gh/prl family, is produced in the intermediate lobe of the teleost pituitary. the function of this protein is uncertain. clones coding for sl were isolated and sequenced from a gilthead seabream pituitary cdna expression library. the nucleotide sequence of the larger cdna isolated was 1.5 kb containing a 0.8-kb 3'-untranslated region and two potential polyadenylation signals (aataaa). the mature polypeptide is composed of 207 amino acids, ... | 1996 | 8954766 |
| cloning and sequence analysis of a cdna encoding salmon (onchorhynchus keta) liver transglutaminase. | we isolated cdna clones encoding a transglutaminase (tgase: ec 2.3.2.13) from a salmon (onchorhynchus keta) cdna library prepared from the liver. in the cdna sequence combined, an open reading frame coding for a protein of 680 aa was found. the deduced sequence showed a considerable similarity (62.4%) to that of red sea bream tgase. by comparison of sequence similarity to other tgases, the structure of salmon tgase was like tissue type tgases, rather than membrane-associated type or plasma type ... | 1996 | 8987854 |
| growth hormone as an in vitro phagocyte-activating factor in the gilthead sea bream (sparus aurata) | the stimulatory action of growth hormone on gilthead sea bream phagocyte-enriched cultures was demonstrated in vitro for the first time in a fish species. phagocytes consisted mainly of macrophages, with a small number of neutrophils and eosinophils. macrophages were unequivocally identified by their esterase staining and the lack of myeloperoxidase staining. cells primed with recombinant rainbow trout gh showed clear morphological (light- and scanning electron-microscopic) and functional differ ... | 1997 | 9023084 |
| a focus of opisthorchiidosis in germany. | epidemiological investigations were carried out in a focus of opisthorchiidosis in brandenburg state of germany. 9 out of 23 cats harboured adult flukes in their livers. beside opisthorchis felineus, metorchis bilis was found in 4 cats. muscle samples of 227 cyprinid fish belonging to 6 species (rutilus rutilus, scardinius erythrophthalmus, alburnus alburnus, abramis brama, a. ballerus, blicca bjoerkna) were examined for trematode metacercariae. 12 different types of cysts were found. o. felineu ... | 1996 | 9060173 |
| primary structure of three forms of gonadotropin-releasing hormone (gnrh) from the pacu brain. | perchlike fish are a vast group of advanced teleosts. the species examined to date have three forms of gonadotropin-releasing hormone (gnrh) within a single species, but the origin of the third gnrh peptide is unknown. in this study, the primary structure of three gnrh peptides is determined from the brain of the pacu, piaractus mesopotamicus, an example of a teleost that is less advanced than the perchlike fish. the gnrh was purified from pacu brain extracts using high performance liquid chroma ... | 1997 | 9100286 |
| levels of chlorinated hydrocarbons (chc) in breams (abramis brama) from the river elbe (a contribution to the federal environmental specimen bank). | the levels of chlorinated hydrocarbon (chc) contaminants in muscle and liver tissues of bream (abramis brama) along the river elbe were monitored during 1991, 1993 and 1994. especially fish from the eastern sampling sites (prossen, dresden) has been heavily contaminated by hcb, ocs, ddt-metabolites and pcbs. in 1991, the concentrations of ocs, dde and pcbs decreased significantly by 65, 65 and 51%, respectively, between dresden and vockerode and did not change very much from vockerode downstream ... | 1997 | 9159913 |
| cross-reactivity of anti-yellowtail thymic lymphocyte monoclonal antibody (yet-2) with lymphocytes from other fish species. | the monoclonal antibody yet-2, generated in mice hyper-immunized with thymic lymphocytes of the yellowtail, seriola quinqueradiata, reacts with the major population of peripheral blood lymphocytes, which might be putative t cells. in this study, we examined the cross-reactivity of yet-2 with lymphocytes from various fish species. flow cytometric analysis showed that yet-2 reacts with 69.8% lymphocytes in the thymus, 89.7% in the peripheral blood, 87.5% in the spleen, and 59.7% in the head-kidney ... | 1997 | 9161694 |
| ceratomyxa sparusaurati (protozoa:myxosporea) infections in cultured gilthead sea bream sparus aurata (pisces:teleostei) from spain: aspects of the host-parasite relationship. | ceratomyxa sparusaurati infection was studied in gilthead sea bream from different mariculture systems of spain. culture conditions were found to influence the infection dynamics. total prevalences ranged from 0 in a closed system to 59.9% in a mediterranean semi-intensive farm. prevalence was significantly lower in summer in a restricted group from the latter system, but no clear seasonal pattern was observed in the remaining fish groups in any system. a statistically significant dependence bet ... | 1997 | 9211504 |
| similarities and differences between the massive eel (anguilla anguilla l.) devastations that occurred in lake balaton in 1991 and 1995. | in the past few years, two massive eel (anguilla anguilla l.) devastations occurred in lake balaton, hungary. in 1991, 300 tons of eel perished in the western basin of the lake, while in the summer of 1995 30 tons of eel died in the eastern part of the lake. investigations carried out to find the causes of these ecocatastrophes included measurements of certain biochemical parameters: the blood sugar level, and the acetylcholinesterase (ache, ec 3.1.1.7), lactate dehydrogenase (ldh, ec 1.1.2.3), ... | 1997 | 9212331 |
| cloning and sequencing of a full-length sea bream (sparus aurata) beta-actin cdna. | a full-length cdna clone encoding beta-actin (beta-actin) was isolated from a sea bream (sparus aurata) liver cdna library. sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. the sea bream beta-actin sequence showed 98% identity to carp and human beta-actin and 95% and 94% identity to sea squirt and dictyostelium cytoplasmic actins, respectively. | 1997 | 9226879 |
| weight-length relationships and reproduction of bream (abramis brama l.) in keszthely bay of lake balaton. | the weight-length relationships of bream (abramis brama l. 1758, family cyprinidae) were studied in lake balaton, hungary, fish were randomly collected with a gill net from keszthely bay between march and november 1995. the condition of bream was investigated monthly, using morphometric parameters of the body, fecundity estimations, and gonadosomatic indices (gsi). the weight-length relationships show that the body weight of bream increased at an approximately 3.4 times higher rate than its leng ... | 1997 | 9270142 |
| diversity of the third form of complement, c3, in fish: functional characterization of five forms of c3 in the diploid fish sparus aurata. | we have recently shown that sparus aurata, the gilthead sea bream (a diploid species), similarly to rainbow trout (a quasi-tetraploid species), possesses multiple forms of the third form of complement (c3). in the present study we have evaluated the ability of the gilthead sea bream proteins to function as active c3 molecules. all five c3 isoforms could be fixed covalently to sheep erythrocyte ghosts and were able to bind to various complement-activating surfaces in the presence of mgegta. in th ... | 1997 | 9307040 |
| the parasitism of fish from lake constance--a comparison of present and earlier data. | commercial fish (perch, roach, bream, dace, and burbot) were caught below langenargen in 1988-1990. their infestation with cestodes and digenean trematodes was studied. the results were compared with earlier data. the infestation of perch with diplostomum spathaceum and ichthyocotylurus variegatus noted in 1988-1990 had increased as compared with that observed in 1974-1976. the infestation of perch with triaenophorus nodulosus seen in 1990 had increased as opposed to that noted in 1988. the infe ... | 1997 | 9342745 |
| evidence that gonadotropin-releasing hormone (gnrh) functions as a prolactin-releasing factor in a teleost fish (oreochromis mossambicus) and primary structures for three native gnrh molecules. | three forms of gonadotropin-releasing hormone (gnrh) are isolated and identified here by chemical sequence analysis for one species of tilapia, oreochromis niloticus, and by hplc elution position for a second species of tilapia, o. mossambicus. of the three gnrh forms in o. mossambicus, chicken gnrh-ii (cgnrh-ii) and sea bream gnrh (sbgnrh) are present in greater abundance in the brain and pituitary than salmon gnrh (sgnrh). these three native forms of gnrh are shown to stimulate the release of ... | 1997 | 9390014 |
| identification and spatial distribution of the mrna encoding the gp49 component of the gilthead sea bream, sparus aurata, egg envelope. | a cdna encoding the precursor of one of the major components of gilthead sea bream, sparus aurata, egg envelope has been cloned by reverse transcriptase polymerase chain reaction (rt-pcr) techniques. the clone was isolated starting from total rna extracted from the liver of spawning female fish and estradiol-17 beta-treated male fish. sequence analysis revealed that the cdna encoded a protein of 405 aa corresponding to 49-kda component (termed gp49), a glycoprotein belonging to the n-linked type ... | 1998 | 9406196 |
| purification of nadph-linked and nadh-linked quinone reductases from liver cytosol of sea bream, pagrus major. | quinone reductases in sea bream, pagrus major, were investigated using menadione as a model quinone. both nadph-linked and nadh-linked quinone reductase activities were detected, in varying degrees, in all tissues examined. in the liver, these activities resided in its microsomal and cytosolic fractions. the cytosolic activity was markedly inhibited by cupric sulfate and p-chloromercuribenzoate. however, little effect was observed with dicoumarol, a potent inhibitor of dt-diaphorase. the nadh-li ... | 1997 | 9467879 |
| chemotaxis of pathogenic vibrio strains towards mucus surfaces of gilt-head sea bream (sparus aurata l.). | vibrio anguillarum and vibrio alginolyticus exhibited significant adhesion to and chemotactic abilities towards mucus collected from the skin, gills, and intestine of gilt-head sea bream. quadratic polynomial models for chemotaxis designed to estimate the influence of temperature demonstrated a differential bacterial chemotaxis depending of the source of the mucus, with the chemotaxis towards intestinal mucus being the least influenced. | 1998 | 9575135 |
| [characteristics of fats of some foods of the meats group and their relations with health]. | the fatty acids and cholesterol contents of ten fresh protein foods were determined, 6 meats (pork, rabbit, lamb, turkey, chicken and beef) and 4 fishes (tuna, sea bream, hake and sardine); the fat quality by different chemical index of the foods (saturated fat/polyunsaturated fat ratio, index of atherogenicity and index of thrombogenicity) and the effect of their intakes on the health were also determined. the study shows that the fat content was lower in meat pork than lamb, rabbit, tuna, sea ... | 1998 | 9644949 |
| rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction. | a simple and sensitive polymerase chain reaction (pcr) based assay is described for detection of the red sea bream iridovirus (rsiv) in infected fish. the assay involves amplification of a portion of the ribonucleotide reductase small subunit (rnrs) gene of the virus from dna isolated from the spleen. the system was tested on red sea bream following an experimental infection. in our infection model, disease signs first became apparent 5 to 6 d post-infection, and mortality commenced at day 6 and ... | 1998 | 9676250 |
| overproduction of dnaj in escherichia coli improves in vivo solubility of the recombinant fish-derived transglutaminase. | the overexpression of red sea bream (pagrus major) transglutaminase (tgase, e.c. 2.3.2.13) in escherichia coli mostly leads to the accumulation of biologically inactive enzyme. although the solubility of the gene products could be improved by cultivation at a lower temperature (26-28 degrees c), most of the synthesized tgase was still in the form of insoluble aggregates. the effects of overproduction of molecular chaperones on the intracellular solubility of newly produced recombinant tgase were ... | 1998 | 9692205 |
| parathyroid hormone-related protein in lower vertebrates. | 1. parathyroid hormone-related protein (pthrp) is an important mediator of humoral hypercalcaemia of malignancy in humans. normal human subjects have very low levels of pthrp in their circulation. 2. parathyroid hormone-related protein has recently been demonstrated in high levels in the circulation and tissues of the sea bream and the dogfish, leading to the hypothesis that pthrp may be a 'classical' hormone in fish. 3. immunohistochemistry and in situ hybridization were performed to investigat ... | 1998 | 9750969 |
| possible transmission of streptococcus iniae from wild fish to cultured marine fish. | streptococcus iniae was isolated from diseased wild fish collected near a mariculture facility where gilthead sea bream and european sea bass exhibited a similar infection. species-specific pcr and ribotyping confirmed that wild and cultured fish were infected by a single s. iniae clone. wild fish are therefore potential amplifiers of pathogenic s. iniae strains. | 1998 | 9758844 |
| ecology of proteocephalus torulosus in the blue bream (abramis ballerus) fromthe oder river on the borders of germany and poland | during studies on the ecology of fish helminths, the tapeworm proteocephalus torulosus (batsch, 1786) was frequently found in the intestine of the blue bream (abramis ballerus) from the oder river (germany/poland). in total, 633 fish, ranging between two and 16 years old, were sampled at monthly intervals over a two year period during 1993-1995. statistically significant differences in the seasonal occurrence of the parasite in its fish host were observed. in 1993, the prevalence remained at a h ... | 1998 | 9765375 |
| effects of orally administered bovine lactoferrin on the immune system of healthy volunteers. | a protective effect of bovine lactoferrin (lf) during lethal bacteraemia has been reported in mice. also, protective effects of orally administered bovine lf have been reported in cases of intractable stomatitis in cats and cryptocaryon irritans infection in red sea bream. in this study, we examined the effects of orally administered bovine lf on the immune system of healthy volunteers. ten healthy male volunteers (age range of 31 to 55 years old) were given bovine lf (2 g/body/day) for 4 weeks. ... | 1998 | 9781368 |
| pathogenicity of vibrio alginolyticus for cultured gilt-head sea bream (sparus aurata l.). | the in vivo and in vitro pathogenic activities of whole cells and extracellular products of vibrio alginolyticus for cultured gilt-head sea bream were evaluated. the 50% lethal doses ranged from 5.4 x 10(4) to 1.0 x 10(6) cfu/g of body weight. the strains examined had the ability to adhere to skin, gill, and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. in addition, the in vitro ability of v. alginolyticus to adhere to mucus and skin cells of sea bream ... | 1998 | 9797276 |
| cloning, characterisation and expression of the apolipoprotein a-i gene in the sea bream (sparus aurata). | a full length cdna clone representing apolipoprotein a-i was isolated from a sea bream (sparus aurata) liver library. the clone encodes a 261 amino acid protein which shows highest amino acid identity (38%) with salmon apolipoprotein a-i. northern blot analysis showed strong expression of a 1.4 kb transcript in liver with lower expression in intestine. expression of apolipoprotein a-i in intestine was markedly reduced by treatment with triiodothyronine (t3). | 1998 | 9804999 |
| ectoparasitic species of the genus trichodina (ciliophora: peritrichida) parasitising british freshwater fish. | seven species of the genus trichodina ehrenberg, 1838 were identified during a sampling programme of twenty freshwater fish species from approximately sixty sites in scotland, england and wales. species found include: trichodina acuta lom, 1961 from cyprinus carpio l., carassius auratus l., oncorhynchus mykiss (walbaum), salmo trutta l. and phoxinus phoxinus l.; trichodina domerguei wallengren, 1897 from gasterosteus aculeatus l.; trichodina tenuidens faure-fremiet, 1944 from gasterosteus aculea ... | 1998 | 9805782 |
| sequence and expression of a cdna encoding the red sea bream androgen receptor. | the cdna of the androgen receptor (ar) has been isolated from the ovary of red sea bream, pagrus major, and sequenced. the amino acid sequence of red sea bream ar (rsar) shows about 45% identity with that of xenopus, rat, mouse, and human ar. it is shown that rsar has the ability to trans-activate the responsive gene depending on the presence of androgen. | 1999 | 10082979 |
| experimental studies on the life-cycle of sebekia wedli (pentastomida: sebekidae). | four young nile crocodiles (crocodylus niloticus) were infected with infective pentastome larvae obtained from naturally infected mozambique bream, oreochromis mossambicus, and red-breasted bream, tilapia rendalli swierstrai in the kruger national park. at day 95 post infection one of the crocodiles died and three female and four male s. wedli were recovered from its lungs. one pair was found in copula but the uteri of the females were not yet developed. males and females were of about equal siz ... | 1998 | 10192834 |
| identification of transthyretin in fish (sparus aurata): cdna cloning and characterisation. | transthyretin (ttr) has been proposed to have first evolved in reptiles and is one of the three plasma proteins important in the transport of thyroid hormones in higher vertebrates. a full-length cdna encoding ttr was isolated from a sea bream (sparus aurata) liver cdna library using a homologous ttr cdna probe generated by rt-pcr. comparison of the deduced amino acid sequence of sea bream ttr with other published sequences, revealed an overall identity of 47-54%, although the amino acids in the ... | 1999 | 10218999 |
| identification of serum gh-binding proteins in the goldfish (carassius auratus) and comparison with mammalian gh-binding proteins. | the present study constitutes the characterization of a specific, high-affinity gh-binding protein (ghbp) in the serum of a teleost, the goldfish (carassius auratus). gh-binding assay and ligand blotting techniques were employed to identify ghbps in goldfish serum and hepatocyte culture medium. the binding characteristics and apparent molecular weights (mr) of goldfish ghbps were also compared with those of rabbit and rat. ligand analysis identified a single class of high-affinity and low-capaci ... | 1999 | 10320823 |
| effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test. | since 1990, red sea bream iridovirus (rsiv) has caused high mortalities in the summertime in cultured red sea bream pagrus major in southwest japan. to establish control measures for red sea bream iridoviral disease (rsivd), the effectiveness of a formalin-killed viral vaccine was evaluated in a field trial. two groups each consisting of 1000 juvenile red sea bream were either intraperitoneally inoculated with vaccine (vaccinated group) or were not vaccinated (non-vaccinated group). after vaccin ... | 1999 | 10349554 |
| bioaccumulation of mercury in various fish species from orlík and kamýk water reservoirs in the czech republic. | the study evaluated contamination of fish by mercury in two important czech water reservoirs, orlík and kamýk, which receive water from the whole watershed of otava and vltava rivers. contamination of these reservoirs reflects the situation in southern and western bohemia. six environmentally representative sites were sampled in the area of interest and the evaluation was based on regression models relating hg concentrations in fish tissues to the age of fish. a total number of 170 analyzed indi ... | 1999 | 10381300 |
| epitheliocystis agents in sea bream sparus aurata: morphological evidence for two distinct chlamydia-like developmental cycles. | the morphology of membrane-bound intracellular inclusions, or 'cysts', of epitheliocystis from sea bream sparus aurata is described. inclusions under the light microscope appear either granular or amorphous. granular inclusions do not elicit a proliferative host reaction and contain the 3 distinctive developmental stages of chlamydial organisms: the highly pleomorphic reproductive form or reticulate body, the condensing form or intermediate body and the infective non-dividing rather uniform elem ... | 1999 | 10439904 |
| redescription of myxobolus hungaricus jaczó, 1940 (myxosporea: myxobolidae), a parasite of bream (abramis brama l.). | myxobolus hungaricus jaczó, 1940 forms relatively large plasmodia on the gills of bream (abramis brama). the authors give a redescription of this deficiently described parasite on the basis of spores collected from the original habitat, which spores are indicated as a neotype. the plasmodia of the parasite can be found on the gills typically in the spring months, and are located in the central part of the gill filaments. they start to develop in the lumen of capillaries of the secondary lamellae ... | 1999 | 10497828 |
| the contribution of a pyrethroid insecticide to the massive eel (anguilla anguilla) devastation, in lake balaton, in 1995. | in the summer of 1995, 30 tonnes of eel (anguilla anguilla) died in lake balaton, hungary. an investigation was carried out to find the causes of this ecocatastrophe. during this investigation, certain biochemical parameters, i.e. the blood sugar level, the acetylcholinesterase (ache, ec 3.1.1.7), lactate dehydrogenase (ldh, ec 1.1.2.3), glutamic-oxaloacetic transaminase (got, ec 2.6.1.1), and glutamic-pyruvic transaminase (gpt, ec 2.6.1.2) activities in the blood serum of the collected survivin ... | 1999 | 10574437 |
| [the parasite-host relations in the system of ligula intestinalis (l.) (cestoda, pseudophyllidae)--the bream]. | proteins and reducing sugars were assayed. the total proteolytic and amylolytic activities were determined in flushes of body cavity of the breams infected and noninfected by plerocercoids ligula intestinalis. infection with l. intestinalis markedly affected the composition of the medium and the activity of enzymes functioning in the body cavity of the bream: protein content increased, total proteolytic activity remained practically unchanged, while the content of reducing sugars and total amylo ... | 1999 | 10576859 |