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relationship of pectolytic enzyme production by isolates of sclerotium cepivorum berk to their pathogenicity. 19744479592
fungistatic and bacteriostatic activities of alkamides from heliopsis longipes roots: affinin and reduced amides.this work demonstrates the fungistatic and bacteriostatic activities of affinin, the main alkamide of heliopsis longipes (gray) blake (asteraceae) roots and two alkamides obtained by catalytic reduction of affinin: n-isobutyl-2e-decenamide and n-isobutyl-decanamide. the bioactivity was tested against rhizoctonia solani groups ag3 and ag5, sclerotium rolfsii, sclerotium cepivorum, fusarium sp., vertcillium sp., phytopathogenic fungi; phytophthora infestans, a phytopathogenic chromista; saccharomy ...200415264902
characterisation of a trichoderma hamatum monooxygenase gene involved in antagonistic activity against fungal plant pathogens.a monooxygenase gene was isolated from a biocontrol strain of trichoderma hamatum and its role in biocontrol was investigated. the gene had homologues in other fungal genomes, but was not closely related to any fully characterised gene. the t. hamatum monooxygenase gene was expressed specifically in response to the plant pathogens sclerotinia sclerotiorum, sclerotinia minor and sclerotium cepivorum, but not in response to botrytis cinerea or t. hamatum. expression of the gene did not occur until ...200818231791
the degradation of onion cell walls by extracellular enzymes of botrytis allii and sclerotium cepivorum [proceedings]. 1978565727
production of macrosphelide a by the mycoparasite coniothyrium minitans.coniothyrium minitans, a mycoparasite of sclerotia of sclerotinia sclerotiorum and sclerotium cepivorum, produced four closely related metabolites inhibitory to fungal growth. the major metabolite, identified as macrosphelide a, had ig(50) values (the concentration of metabolite to inhibit growth by 50%) of 46.6 and 2.9 microgram ml(-1) against s. sclerotiorum and s. cepivorum, respectively. this is the first report of both antifungal activity due to macrosphelide a as well as isolation of macro ...200312594019
synthesis and antifungal activity of some new quinazoline and benzoxazinone derivatives.the hitherto unknown 2-isopropyl-6,8-dibromo-4h-3,1-benzoxazin-4-one (2) was subjected to condensation with either primary or secondary amines affording the benzamide derivatives (3-7), while with alcohols in presence of the base, corresponding esters were obtained (8 and 9). acylation of the hydrazide (12) or its cyclized form (13) gave (14-17). the quinazolinone derivative (18) was obtained either when (12) was reacted with nitrous acid or via fusion of (2) with ammonium acetate. the thione (2 ...200011129978
a group-i intron in the mitochondrial small subunit ribosomal rna gene of sclerotinia sclerotiorum.a 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal rna (mt ssu rrna) gene of the fungus sclerotinia sclerotiorum has been sequenced and identified as a group-i intron. this is the first report of an intron in the mt ssu rrna gene. the intron shows close similarity in secondary structure to the subgroup-ic2 introns from podospora (nd3i1, nd5i2, and coii5) and neurospora (nd5i1). the intron has an open reading frame (orf) that encodes a putative protein of 420 amino a ...19957788720
vital fluorochromes as tracers for fungal growth studies.eight fluorescent dyes were tested for staining the spores or mycelia of six fungi and for their translocation into new growth when the preloaded spores or mycelia were incubated on agar coated coverslips. the dyes studied were cellufluor, nile red, fluorescein diacetate (fda), carboxyfluorescein diacetate (cfda), chloromethylfluorescein diacetate (cmfda), aminochloromethyl coumarin (cmac), and the carbocyamines diic18(3) and dioc18(3). the fungi on which the dyes were tested included botrytis c ...19957578589
mycoparasitism of sclerotial fungi by teratosperma oligocladum.sclerotia of sclerotinia minor were parasitized by teratosperma oligocladum, a recently described dematiaceous hyphomycete. the mycoparasite was cultured on living sclerotia placed on water agar and on sclerotia in moist sand. it grew poorly on several common laboratory media but growth in vitro was enhanced by supplements of soil extract and, especially, by aqueous extracts of sclerotia. sclerotia of s. minor, s. sclerotiorum, s. trifoliorum, sclerotium cepivorum, and botrytis cinerea were para ...19817198002
effect of some antibiotics on sclerotium cepivorum berk., the cause of white rot of onion. 19715129427
Antifungal saponins from bulbs of white onion, Allium cepa L.Three saponins, named ceposide A, ceposide B, and ceposide C were isolated from the bulbs of white onion, Allium cepa L. Elucidation of their structure was carried out by comprehensive spectroscopic analyses, including 2D NMR spectroscopy and mass spectrometry, and chemical evidences. The structures of the compounds were identified as (25R)-furost-5(6)-en-1ß,3ß,22a,26-tetraol 1-O-ß-d-xylopyranosyl 26-O-a-d-rhamnoyranosyl-(1?2)-O-ß-d-galactopyranoside (ceposide A), (25R)-furost-5(6)-en-1ß,3ß,22a, ...201122169018
recombinant amaranth cystatin (ahcpi) inhibits the growth of phytopathogenic fungi.phytocystatins are cysteine proteinase inhibitors from plants implicated in defense mechanisms against insects and plant pathogens. we have previously characterized an amaranth cystatin cdna and analyzed its response to different kinds of abiotic stress [37]. in order to characterize amaranth cystatin, the coding sequence was expressed in escherichia coli using the pqe-2 vector. recombinant cystatin was predominantly found in the soluble fraction of the cell extract. large amounts (266 mgl(-1)) ...201020403704
phylogenetic placement of plant pathogenic sclerotium species among teleomorph genera.phylogenetic analyses and morphological characteristics were used to assess the taxonomic placement of eight plant-pathogenic sclerotium species. members of this genus produce only sclerotia and no fruiting bodies or spores, so sclerotium species have been difficult to place taxonomically. sequences of rdna large subunit (lsu) and internal transcribed spacer (its) regions were determined for isolates of sclerotium cepivorum, s. coffeicola, s. denigrans, s. hydrophilum, ceratorhiza oryzae-sativae ...201020361501
clonal spread of sclerotium cepivorum in onion production with evidence of past recombination events.three field populations of sclerotium cepivorum from ontario and que-bec in canada, one field sample from the north island of new zealand, as well as isolates from other onion- and garlic-growing areas were classified in mycelial compatibility groups (mcgs) and further characterized by dna fingerprints and dna sequence polymorphisms at six genomic regions. mcgs in the population samples were genetically distinct clones. members of a clone belonged to a unique mcg with an associated multi-locus g ...200018944558
allium white rot suppression with composts and trichoderma viride in relation to sclerotia viability.abstract allium white rot (awr) is a serious disease of allium spp. caused by the sclerotium-forming fungus sclerotium cepivorum. this work has examined the effects of onion waste compost (owc) and spent mushroom compost (smc), with and without trichoderma viride s17a, on sclerotia viability and awr in glasshouse and field experiments. incorporation of owc into soil reduced the viability of sclerotia and the incidence of awr on onion plants in glasshouse pot bioassays, whereas smc or t. viride s ...200618944057
[pathogenic characterization and study of the mycelial groups of compatibility in sclerotium cepivorum berk].we have studied the pathogenicity and the formation of mycelial compatibility groups of (mcg) in 12 isolates of the fungus sclerotium cepivorum berk from the states of táchira, mérida, trujillo in venezuela and pamplona in colombia. the pathogenicity was evaluated according to severity and virulence. in vitro confrontation tests were used to check the presence of compatible and incompatible interactions expressed by mcg when different isolates of the fungus were put together in a petri dish. whe ...200212828515
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