| capillary and gel electromigration techniques and maldi-tof ms--suitable tools for identification of filamentous fungi. | microbial strains are now spreading out of their original geographical areas of incidence and previously adequate morphological identification methods often must be accompanied by a phenotypic characterization for the successful microbial identification. the fungal genus monilinia represents a suitable example. monilinia species represent important fruit pathogens responsible for major losses in fruit production. four closely related spp. of monilinia: monilinia laxa, monilinia fructigena, monil ... | 2011 | 22284891 |
| expression, characterization and 2,4,6-trichlorophenol degradation of laccase from monilinia fructigena. | a novel laccase gene from monilinia fructigena was synthesized chemically according to the yeast bias codon and integrated into the genome of pichia pastoris gs115 by electroporation. the expressed enzyme was recovered from the culture supernatant and purified. the result of enzyme activity assay and sds-page demonstrated that the recombinant laccase was induced and extracellularly expressed in p. pastoris. main biochemical properties of this laccase, such as thermodependence and thermostability ... | 2011 | 21743993 |
| effect of temperature and water activity on in vitro germination of monilinia spp. | this study evaluated the effect of temperature (0-38 degrees c) and water activity (a(w): 0.87-0.99) on the lag phase prior to germination and the percentage of germination over time for monilinia laxa, monilinia fructicola and monilinia fructigena. | 2010 | 19519666 |
| temporal dynamics of brown rot in different apple management systems and importance of dropped fruit for disease development. | abstract epidemic development of brown rot, caused by monilinia fructigena, was monitored in integrated and organic apple orchards at two locations in eastern hungary between 2002 and 2005 on three cultivars with early, midseason, and late ripening periods. disease incidence and severity measures were affected significantly (p < 0.05) by management system (organic versus integrated) and cultivar, but there was no significant management system-cultivar interaction. epidemics started 2 to 4 weeks ... | 2007 | 18944175 |
| quantitative relationships between different injury factors and development of brown rot caused by monilinia fructigena in integrated and organic apple orchards. | in a 4-year study, the incidence of various types of injuries (caused by insects, birds, growth cracks, mechanical wounding, and other, unidentified factors) was assessed in relation to brown rot development (caused by monilinia fructigena) on fruit of three apple cultivars (prima, jonathan, and mutsu) in integrated and organic blocks of two apple orchards in hungary. in addition, populations of male codling moths (cydia pomonella) were monitored with pheromone traps season-long in both manageme ... | 2008 | 18943241 |
| in vitro and in vivo [corrected] activity of eugenol oil (eugenia caryophylata) against four important postharvest apple pathogens. | the activity of eugenol oil was evaluated in vitro and in vivo against four apple pathogens namely phlyctema vagabunda, penicillium expansum, botrytis cinerea and monilinia fructigena. the minimum inhibitory concentration (mic) of eugenol incorporated in malt extract agar medium was found to be 2 mg ml(-1). mycelial growth of the four test pathogens was completely inhibited when treated with 150 microl l(-1) of volatile eugenol whether at 4 or 20 degrees c. conidia of p. vagabunda, p. expansum, ... | 2008 | 18554737 |
| syntheses of lambertellols and their stable analogues; investigation of the real active species in the mycoparasitism by lambertella species. | lambertellols a (1) and b (2), isolated from mycoparasites lambertella species, were synthesized. the synthesis features intramolecular aldol-type cyclizations of aldehydes 12 and 14 and site specific oxidations of 1-hydroxylambertellols as key steps. the synthesis also provided all diastereomers of 1-hydrolambertellols 17-19 and 25. chiral resolution made the optically active forms available, which enabled the investigation of the real active species in the mycoparasitism by lambertella species ... | 2008 | 18522417 |
| multiplex pcr for species discrimination of sclerotiniaceae by novel laccase introns. | common pcr-based targets for the identification of filamentous fungi and yeasts are the sequences of the internal transcribed spacer region (its1, 5.8s rdna, its2). within the sclerotiniaceae the its-region is homogenous and the identification is almost impossible. furthermore, the lack of igs-data (intergenic spacer region) requires new specific marker genes for a rapid identification of phytopathogenic sclerotiniaceae. we sequenced and analyzed new laccase2 (lcc2) genes from the phylogenetical ... | 2007 | 17706821 |
| stimulation of the biosynthesis of the antibiotics lambertellols by the mycoparasitic fungus lambertella corni-maris under the acidic conditions produced by its host fungus in vitro. | the filamentous fungus, lambertella corni-maris (l. corni-maris), a mycoparasite on monilinia fructigena, produces the antibiotics, lambertellols a (1), b (2), and lambertellin (3), in a substantial amounts under acidic conditions, whereas these antibiotics were hardly detected when the fungus was cultured on a potato-sucrose (ps) medium without added acids. our investigations also revealed that the host, m. fructigena, changed its surroundings into acidic conditions, suggesting that the acidic ... | 2007 | 17485857 |
| investigation of the radiation effects on brown rot disease of golden delicious apples, inoculated with the fungus monilinia fructigena. | the effects of 0, 1.5 and 3 kgy gamma-irradiation on brown rot disease affecting golden delicious apples are investigated. the apples were artificially inoculated with monilinia fructigena fungus before and after irradiation. results show clearly that radiation induces a delay in fungus development, even when it is not directly affected. the effect of radiation on the apples' substrate seems to be considerable, being the main factor affecting fungal growth. radiation doses of 1.5 and 3 kgy do no ... | 1998 | 16284856 |
| using survival analysis to investigate the effect of uv-c and heat treatment on storage rot of strawberry and sweet cherry. | ultraviolet light and heat treatment are proposed as alternative techniques for the use of chemicals to reduce the development of the spoilage fungi botrytis cinerea and monilinia fructigena on strawberry and sweet cherry, respectively, during storage. in order to investigate the effect of both physical techniques on microbial inactivation and on fruit quality, inoculated berries were subjected to different temperatures (40-48 degrees c) and uv-c doses (0.05-1.50 j/cm2). for each condition, 20 b ... | 2002 | 11934026 |
| inactivation of conidia of botrytis cinerea and monilinia fructigena using uv-c and heat treatment. | the effect of uv-c (lambda = 254 nm) and heat treatment was investigated on the inactivation of conidia of botrytis cinerea and monilinia fructigena, two major postharvest spoilage fungi of strawberries and cherries, respectively. both fungi were grown at 21 degrees c in the dark and conidia were isolated after 1 week by washing the mycelium with a mild detergent solution. after filtration and resuspension in phosphate buffer to a titer of 10(5) to 10(6) cfu/ml, the conidia were subjected to dif ... | 2002 | 11930952 |
| purification and mechanistic properties of an extracellular alpha-l-arabinofuranosidase from monilinia fructigena. | 1. the alpha-l-arabinofuranosidase isoenzyme designated afiii [laborda, archer, fielding & byrde (1974) j. gen. microbiol. 81, 151-163] was purified by sequential isoelectric focusing, hydrophobic chromatography, gel filtration and chromatofocusing. 2. the enzyme is a monomer of mr 40,000. 3. on inactivation of the enzyme with 3h-labelled 1-alpha-l-arabinofuranosylmethyl-3-p-nitrophenyltriazene, 0.64 mol of alpha-l-arabinofuranosylmethyl residues/mol of enzyme is estimated to become attached to ... | 1987 | 3663195 |
| one-proton catalysis by the alpha-l-arabinofuranosidase iii of monilinia fructigena. | 1. michaelis-menten parameters for the hydrolysis of p-nitrophenyl alpha-l-arabinofuranoside were measured as a function of pl (ph or pd) in both 1h2o and 2h2o. 2. the variation of both vmax. and vmax./km with pl is sigmoid, the pk governing vmax. shifting from 6.34 +/- 0.05 in 1h2o to 6.84 +/- 0.07 in 2h2o, and that governing vmax./km from 5.89 +/- 0.03 in 1h2o to 6.38 +/- 0.05 in 2h2o. 3. in the plateau regions there is a small inverse solvent isotope effect on vmax./km (0.92), and one of 1.45 ... | 1988 | 2848501 |
| inhibition of the alpha-l-arabinofuranosidase iii of monilinia fructigena by 1,4-dideoxy-1,4-imino-l-threitol and 1,4-dideoxy-1,4-imino-l-arabinitol. | 1. 1,4-dideoxy-1,4-imino-l-threitol was synthesized and the synthesis of 1,4-dideoxy-1,4-imino-l-arabinitol was improved. 2. both compounds are competitive inhibitors of monilinia fructigena alpha-l-arabinofuranosidase iii, the additional hydroxymethyl group in the arabinitol contributing about 17.8 kj/mol (4.25 kcal/mol) to the gibbs free energy of binding. 3. the affinities (1/ki) of both compounds vary with ph in a classical bell-shaped way, the pka value being that of the acid-catalytic grou ... | 1990 | 2310375 |