dna relatedness among erysipelothrix rhusiopathiae strains representing all twenty-three serovars and erysipelothrix tonsillarum. | the levels of relatedness among strains of erysipelothrix rhusiopathiae (serovars 1 through 23 and type n) were estimated by performing dna-dna hybridization experiments with the type strains of e. rhusiopathiae and erysipelothrix tonsillarum, which are the two erysipelothrix species that have been described. two distinct dna relatedness groups were identified. the group 1 strains, representing serovars 1, 2, 4 through 6, 8, 9, 11, 12, 15 through 17, 19, and 21 and type n, exhibited more than 73 ... | 1992 | 1503975 |
classification of erysipelothrix strains on the basis of restriction fragment length polymorphisms. | restriction fragment length polymorphisms of the 16s rrna genes of erysipelothrix strains were studied by cleavage of the chromosomal dna with restriction endonuclease ecori, followed by hybridization to a 420-bp internal fragment of the 16s rrna gene. thirty-two erysipelothrix type and reference strains were classified, together with seven field strains. reference strains of all serotypes and the type strains of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum were included. nine rib ... | 1995 | 7537073 |
cellular fatty acid composition of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum. | the cellular fatty acid compositions in 6 strains of erysipelothrix rhusiopathiae and 7 strains of erysipelothrix tonsillarum were determined by gas chromatography. fatty acids, ranging from c10 to c18, were detected in the test strains. the fatty acid profile was characterized by very high percentages of 18:1 (cis-9) (cis-9-octadecenoic acid; 72.4 to 82.1%) and 16:1 (hexadecenoic acid; 8.7 to 13.7%). the profiles of the e. rhusiopathiae and e. tonsillarum strains resembled each other, indicatin ... | 1994 | 8075232 |
analysis of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum by multilocus enzyme electrophoresis. | the genetic diversity of 74 australian field isolates of erysipelothrix rhusiopathiae and 22 reference strains for serovars of e. rhusiopathiae or erysipelothrix tonsillarum was examined by multilocus enzyme electrophoresis. four serovar reference strains of e. tonsillarum (strains ks 20 a, wittling, lengyel-p, and bano 107 for serovars 25, 3, 10, and 22, respectively) were genetically distinct from e. rhusiopathiae. however, the e. tonsillarum reference strain for serovar 14 (iszap-4) and the r ... | 1994 | 8150944 |
differentiation of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cell proteins. | the protein patterns of whole cells of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum were studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the protein patterns of the 16 strains of e. rhusiopathiae and e. tonsillarum studied, including the type strains of these two species, resembled each other, except that there were 71-, 41-, 34-, and 26-kda proteins in the e. rhusiopathiae pattern and 74-, 44-, 36-, and 25-kda proteins in the e. tonsillarum pattern. th ... | 1993 | 8427802 |
erysipelothrix tonsillarum isolated from dogs with endocarditis in belgium. | five strains of erysipelothrix tonsillarum were isolated from dogs with endocarditis in belgium. the identity and validity of the species was proved by serotyping, and biochemical and pathogenicity tests. all the isolates belonged to serovar 7 (e tonsillarum serovars); they produced acid from saccharose but did not induce any clinical sign of erysipelas in swine. these results suggest that some strains of e tonsillarum are a canine pathogen. | 1993 | 8460272 |
serovars of erysipelothrix strains isolated from pigs affected with erysipelas in japan. | serovars of 1,046 erysipelothrix strains isolated from diseased pigs during a period from 1983 to 1993 in japan were determined by an agar gel double-diffusion precipitation system using typing sera representing all the known serovars, 1 through 23 and type n, of 2 species of erysipelothrix. a total of 943 strains could be serotyped within the serovars. the remaining 103 strains could not be determined for their serovars and were classified as untypable. of the 938 (99.5%) strains serologically ... | 1996 | 8811634 |
occurrence of erysipelothrix spp. in broiler chickens at an abattoir. | from september 1995 to august 1996, 750 chickens from 66 farms sent to an abattoir in nagano prefecture, japan, were examined for the presence of erysipelothrix spp. erysipelothrix spp. were isolated from 118 (15.7%) of 750 skin samples, 27 (7.3%) of 372 hypoderm samples, 12 (1.9%) of 630 throat samples, 106 (59.2%) of 179 feather samples, and none of 257 spleen samples. of 66 farms, 55 farms (83.3%) sent erysipelothrix-positive chickens and 11 farms (16.7%) only negative ones. of 297 erysipelot ... | 1998 | 9678179 |
serotyping and pathogenicity of erysipelothrix strains isolated from tonsils of slaughter pigs in thailand. | erysipelothrix strains were isolated from the tonsils of 46 (15.0%) of 307 apparently healthy slaughter pigs in thailand during the period of august to september, 1997. a total of 27 of the 46 erysipelothrix isolates could be classified into 5 serovars but the remaining 19 were untypable in this study. of the 25 isolates serologically identified as erysipelothrix rhusiopathiae, 20, 4, and 1 isolates belonged to serovars 2, 12, and 17, respectively. only 2 isolates from the tonsils belonged to er ... | 1999 | 10535518 |
comparison of methods for detection of erysipelothrix spp. and their distribution in some australasian seafoods. | for many years, erysipelothrix rhusiopathiae has been known to be the causative agent of the occupationally related infection erysipeloid. a survey of the distribution of erysipelothrix spp. in 19 australasian seafoods was conducted, and methodologies for the detection of erysipelothrix spp. were evaluated. twenty-one erysipelothrix spp. were isolated from 52 seafood parts. primary isolation of erysipelothrix spp. was most efficiently achieved with brain heart infusion broth enrichment followed ... | 2000 | 10788383 |
taxonomic evidence that serovar 7 of erysipelothrix strains isolated from dogs with endocarditis are erysipelothrix tonsillarum. | the levels of relatedness among strains of erysipelothrix serovar 7 isolated from dogs with endocarditis were estimated by performing dna-dna hybridization experiments with the type strains of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum. all the canine strains exhibited more than 81% hybridization with the type strain of e. tonsillarum but less than 13% hybridization with the type strain of e. rhusiopathiae. based on dna-dna hybridization results we confirmed that serovar 7 of th ... | 2000 | 10861200 |
phylogenetic analysis of the erysipelothrix rhusiopathiae and erysipelothrix tonsillarum based upon 16s rrna. | the nucleotide sequences of 16s rrna genes of erysipelothrix rhusiopathine and erysipelothrix tonsillarum were determined. the sequences are almost similar (99.8%) with only three nucleotides mismatched. | 2000 | 11092736 |
computerized comparison of the protein compositions of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum strains. | protein profiles of six erysipelothrix rhusiopathiae strains, five erysipelothrix tonsillarum strains and three erysipelothrix strains of uncertain taxonomic position were studied by sodium dodecyl sulphate-polyactylamide gel electrophoresis (sds-page). in a computerized comparison of the protein patterns of the strains, the level of similarity between the strains was determined. the sds-page protein bands were divided into 14 groups based on molecular weight. the relative distribution of protei ... | 2001 | 11254101 |
serovars of erysipelothrix species isolated from the tonsils of healthy cattle in japan. | serovars of 79 erysipelothrix isolates recovered from the tonsils of healthy slaughtered cattle over a 1-year period in japan were determined by an agar double-diffusion precipitation system using typing sera representing all the known serovars, 1 through 23 and type n, of erysipelothrix. a total of 43 out of the 79 erysipelothrix isolates could be classified into nine serovars but the remaining 36 isolates were untypable. of 42 isolates identified as erysipelothrix rhusiopathiae, 4, 6, 2, 3, 1, ... | 2001 | 11423200 |
erysipelothrix inopinata sp. nov., isolated in the course of sterile filtration of vegetable peptone broth, and description of erysipelotrichaceae fam. nov. | based on 16s rrna gene sequence comparison, an isolate that was detected in sterile-filtered vegetable broth was classified as a novel member of the erysipelothrix line of descent of the firmicutes. strain mf-ep02t resembles members of the two species of erysipelothrix with validly published names, erysipelothrix rhusiopathiae and erysipelothrix tonsillarum, in morphology, fatty acid composition, lack of menaquinones in aerobically and anaerobically grown cultures, dna g+c content and peptidogly ... | 2004 | 14742484 |
a multiplex polymerase chain reaction for discriminating erysipelothrix rhusiopathiae from erysipelothrix tonsillarum. | erysipelothrix rhusiopathiae is the causative agent of swine erysipelas, and it causes great economic losses in japan and worldwide. in meat inspection, it is very important to distinguish e. rhusiopathiae from other bacteria showing similar clinical signs of disease or similar bacterial characteristics. to distinguish e. rhusiopathiae from erysipelothrix tonsillarum, 2 polymerase chain reaction (pcr) systems were combined. the primer sets ery-1f and ery-2r were designed to amplify 2210 base pai ... | 2006 | 16921879 |
comparison of the pathogenicity for chickens of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum. | twenty-two strains of erysipelothrix rhusiopathiae, 14 strains of erysipelothrix tonsillarum, and four other strains representing two serovars were examined for virulence in 30- to 40-day-old specific pathogen-free chickens. some of the chickens that were given strains of serovars la, 2, 5, 6, 8, 9, 15 or 21 of e. rhusiopathiae showed signs of disease, lesions, bacteraemia or recovery of the bacterium. in contrast, there were no clinical signs, pathological lesions or bacterial isolations in chi ... | 1994 | 18671089 |
evaluation on the pathogenicity of erysipelothrix tonsillarum for pigs by immunosuppression with cyclophosphamide or dexamethasone. | the pathogenicity of erysipelothrix tonsillarum was evaluated in pigs immunosuppressed with cyclophosphamide (cy) or dexamethasone (dm). animals were treated with 15 mg/kg cy (n=8, five injections), 1 mg/kg dm (n=8, nine injections) or left untreated (n=8). on the fifth day after the beginning of drug treatments, swine were inoculated with one of two e. tonsillarum serovar 7 strains (approximately 10(6) cfu per pig). in the cy-treated group, both circulating neutrophil and lymphocyte counts decr ... | 2011 | 20570299 |
erysipelothrix rhusiopathiae contamination in the poultry house environment during erysipelas outbreaks in organic laying hen flocks. | this study investigated organic laying hen farms for the presence of erysipelothrix rhusiopathiae in the house environment and from potential carriers (i.e. insects and mice) during ongoing erysipelas outbreaks, and compared the obtained isolates with those from laying hens. the samples were investigated by selective culture followed by species-specific polymerase chain reaction on cultures. e. rhusiopathiae was isolated from the spleen, jejunal contents, manure, dust and swabs from water nipple ... | 2014 | 24661145 |
serotypes and spa types of erysipelothrix rhusiopathiae isolates from british pigs (1987 to 2015). | erysipelothrix spp. cause a range of clinical signs in pigs and at least 28 different erysipelothrix spp. serotypes have been identified. in this study, 128 isolates of erysipelothrix spp. from pigs in great britain from 1987 to 2015 were characterised by serotyping and multiplex real time pcr assays targeting the surface protective antigen (spa) and the main genotypes (erysipelothrix rhusiopathiae, erysipelothrix tonsillarum and erysipelothrix spp. strain 2). all 128 british isolates were chara ... | 2017 | 28720292 |