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serological response to "rochalimaea henselae" antigen in suspected cat-scratch disease.there are no generally accepted diagnostic tests for cat-scratch disease (csd), the cause of which is unknown. during the development of an indirect fluorescence antibody (ifa) test for detection of antibodies to "rochalimaea henselae", sera from patients with csd were found to have high titres to r henselae antigens. further tests with this assay showed that 36 (88%) of 41 patients with suspected csd had serum titres of 64 or more to r henselae antigen, that there was a low prevalence (3%) of s ...19921351130
naturally occurring "rochalimaea henselae" infection in domestic cat. 19921354314
possible role of rochalimaea henselae in pathogenesis of aids encephalopathy. 19921357365
isolation of rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis.bacillary angiomatosis is characterized by vascular lesions, which occur usually in patients infected with the human immunodeficiency virus (hiv). a newly described gram-negative organism, rochalimaea henselae, has been associated with cutaneous bacillary angiomatosis, but no organism has been isolated and cultivated directly from cutaneous tissue.19921435899
immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia.we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ...19921530106
rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ...19921537892
relapsing illness due to rochalimaea henselae in immunocompetent hosts: implication for therapy and new epidemiological associations.two previously healthy, immunocompetent men had persistent rochalimaea henselae bacteremia with clinical relapses after courses of antibiotics to which the isolates were ultimately demonstrated susceptible in vitro. both had sustained tick bites prior to their illnesses, thus demonstrating an association not previously identified, although suspected. the first patient had relapsing fever, constitutional symptoms, and an episode of aseptic meningitis despite therapy with amoxicillin, then with do ...19921562660
murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana.rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ...19921629326
serologic responses to bartonella and afipia antigens in patients with cat scratch disease.to assess the serologic response to afipia and bartonella, previously named rochalimaea, in patients with cat scratch disease (csd) and a healthy control group.19957491236
rochalimaea henselae infection in acquired immunodeficiency syndrome causing inflammatory disease without angiomatosis or peliosis. demonstration by immunocytochemistry and corroboration by dna amplification.rochalimaea henselae causes bacillary angiomatosis, bacillary peliosis, and persistent bacteremia. difficult to cultivate, it is detectable in infected tissues by immunocytochemistry. this technique demonstrated r henselae in autopsy specimens obtained from three deceased patients who had acquired immunodeficiency syndrome, with pathologic tissue changes lacking neoangiogenic features. from the first patient, the cause of nodular collections of lymphocytes and nonepithelioid histiocytes in the l ...19947506902
isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-pcr, 16s rrna gene sequencing, and pulsed-field gel electrophoresis of rochalimaea quintana from a patient with bacillary angiomatosis.rochalimaea quintana was isolated from the blood of a french human immunodeficiency virus-infected patient with bacillary angiomatosis. the isolate showed the typical growth characteristics of rochalimaea species and was inert when typical biochemical testing was used. the purpose of the present work was to characterize and compare this new isolate with reference strains of r. quintana, rochalimaea vinsonii, and rochalimaea henselae by using immunofluorescence, sodium dodecyl sulfate-polyacrylam ...19947519628
[rochalimaea henselae, afipia felis and cat-scratch disease].several years ago, rochalimaea henselae has emerged as an agent of bacillary angiomatosis, bacillary peliosis and recurrent septicaemia that generally occur in patients infected with human immunodeficiency virus. an aetiologic role in cat scratch disease is also suspected widely on the basis of a serologic survey. its slow growth and its culture requirement explain that this pathogen, a gram-negative bacterium, could not be isolated until 1990. moreover, blood and tissue samples request lysis an ...19947524253
cat-scratch disease in italy: a serological approach.the results of studies on the serologic responses to afipia felis and rochalimaea henselae in suspected patients for cat scratch disease (csd) are illustrated. this preliminary study performed using indirect immunofluorescence assay, proved negative for a. felis and r. henselae in some patients and positive in others; in a few instances the test was positive for both organisms. additional microbiological and serological studies are needed to clarify the exact role of these microorganisms in caus ...19947526131
positive predictive value of rochalimaea henselae antibodies in the diagnosis of cat-scratch disease. 19947527255
downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the alpha-2 subgroup of proteobacteria (afipia felis and rochalimaea henselae).intracellular pathogens have evolved effective mechanisms in order to survive in an intracellular environment, thus avoiding destruction by phagocytic cells. in this regard, a correlation between resistance to phagocytic killing and expression of pathogenic potency has been established. in this report, we have studied the interaction between human polymorphonuclear cells (pmn) and two gram-negative microorganisms, afipia felis and rochalimaea henselae, which belong to the alpha-2 subgroup of the ...19947528238
persistent infection with bartonella (rochalimaea) henselae or afipia felis is unlikely to be a cause of chronic fatigue syndrome. 19947528553
dual role for afipia felis and rochalimaea henselae in cat-scratch disease. 19957531265
detection of rochalimaea henselae dna by polymerase chain reaction from suppurative nodes of children with cat-scratch disease.a polymerase chain reaction (pcr) assay was designed to amplify dna from rochalimaea henselae, rochalimaea quintana and afipia felis in the purulent material from lymph nodes in three patients with clinical cat-scratch disease (csd) and two patients with lymphadenitis from other causes. all of the patients with csd had positive immunofluorescent antibody serology for r. henselae, while none of the controls was positive. pcr amplification confirmed the presence of r. henselae dna and the absence ...19947531320
immunoelectrophoretic characterization and cross-reactivity of rochalimaea henselae, rochalimaea quintana and afipia felis.the soluble antigens of rochalimaea henselae, rochalimaea quintana and afipia felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. a precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. by this technique 56 antigens were identified from r. henselae, 49 from r. quintana, and 39 from a. felis. the ...19947534092
antibody response in rabbits infected with rochalimaea henselae, rochalimaea quintana and afipia felis.antibody responses in three pairs of rabbits inoculated with live rochalimaea henselae, rochalimaea quintana and afipia felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. no differences in measured antibody responses were found with the two types of antigen preparation. two rabbits did not respond with antibody production. in the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. af ...19947534093
[cat-scratch disease and disease caused by bartonella (rochalimaea)].the aetiology of cat scratch disease remains controversial since both afipia felis and bartonella (rochalimaea) henselae have been isolated from diseased lymph nodes. bartonella henselae, bartonella (rochalimaea) quintana and bartonella (rochalimaea) elizabethae cause endocarditis and bartonella bacilliformis cause septicemia (oroya's fever) in non-immunocompromized patients, and bartonella henselae and bartonella quintana cause fever, bacillary angiomatosis, and visceral peliosis in human immun ...19957534931
etiology of cat scratch disease: comparison of polymerase chain reaction detection of bartonella (formerly rochalimaea) and afipia felis dna with serology and skin tests.to determine the role of bartonella (formerly rochalimaea) species and afipia felis in cat scratch disease (csd), two polymerase chain reaction (pcr) hybridization assays were developed to detect dna from these organisms. these assays were applied on 89 pus aspirates from skin test-positive csd patients (group 1) and on 137 pus and lymph node specimens from csd suspects (group 2). bartonella dna was detected in 96% of the samples from group 1 patients and in 60% of group 2 samples; however, a. f ...19957535830
a chemically defined liquid medium that supports primary isolation of rochalimaea (bartonella) henselae from blood and tissue specimens.rochalimaea (bartonella) henselae is a fastidious, slowly growing, gram-negative bacillus that is an etiologic agent of bacillary angiomatosis, cat scratch disease, and related syndromes. accumulation of direct microbiologic evidence of the relationship between the organism and the syndromes compatible with cat scratch disease has been hindered by the difficulties in the primary isolation of the organism from infected tissue specimens. a chemically defined liquid medium was developed to support ...19957538511
characterization of a novel rochalimaea species, r. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient.isolation of a rochalimaea-like organism from a febrile patient infected with human immunodeficiency virus was confirmed. analysis of 16s rrna gene sequences, together with polymerase chain reaction and restriction endonuclease length polymorphism analysis of a portion of the citrate synthase gene, demonstrated that the agent is closely related to members of the genus rochalimaea and that the isolate is genotypically identical to the presumptive etiologic agent of bacillary angiomatosis. however ...19921371515
cutaneous bacillary angiomatosis in a patient with chronic lymphocytic leukemia.bacillary angiomatosis is a recently described vascular disorder that is associated with infection by bartonella henselae (formerly known as rochalimaea henselae) and bartonella quintana (formerly known as rochalimaea quintana); this disorder usually occurs in patients with human immunodeficiency virus infection. we report a case of cutaneous bacillary angiomatosis that occurred in a patient with chronic lymphocytic leukemia.19957543264
prevalence of antibody to rochalimaea henselae among austrian cats. 19957536676
[cat scratch (?) disease].the case of a 24 year old female student who developed typical cat scratch disease following an insect sting invited to review the literature. over the last two years, rocalimaea henselae could be identified and is now considered to be responsible for the majority of infected cases. it is related to rochalimaea quintana that provokes trench fever and to bartonella bacilliformis, known as the inciting agent of the verruca peruana and of oroya fever. recently, it has been proposed to integrate roc ...19957533323
[bacillary angiomatosis].bacillary angiomatosis (ba) is a rare infectious disease usually associated with hiv infection. recent molecular biologic investigations confirm that both rochalimaea henselae and rochalimaea quintana can cause ba. the bacteria can be identified by warthin-starry staining and electron microscopy. the typical clinical signs are solitary or multiple dermal or subcutaneous nodules. bone, liver, spleen and other organs may also be involved. we describe the clinical and histological features of a 39- ...19957533145
recurrent pyogenic granuloma with satellitosis--a localized variant of bacillary angiomatosis?a 27-year-old male patient had recurrent pyogenic granuloma with satellitosis. histologically, warthin-starry staining of the lesions revealed clumps of dark bacilli as found in patients with bacillary angiomatosis. igg antibodies against bartonella (rochalimaea) henselae were elevated as shown by an indirect immunofluorescence assay. the patient did not present an obvious risk for hiv infection or immunosuppression, and no antibodies against hiv-1 and hiv-2 were found. recurrent pyogenic granul ...19947533011
differentiation of bartonella-like isolates at the species level by pcr-restriction fragment length polymorphism in the citrate synthase gene.the citrate synthase gene (glta) of bartonella henselae was cloned and sequenced to compare genetic divergence among alpha and gamma branches of the class proteobacteria and to develop enhanced genotypic reagents for b. henselae identification. b. henselae glta is 1,293 nucleotides in length and 63 to 66% homologous with corresponding gene sequences of rickettsia prowazekii, escherichia coli, and coxiella burnetii. the observed genetic variability suggests that glta sequences can provide a usefu ...19957545181
cat scratch disease: past and present. 19947532423
from the centers for disease control and prevention. encephalitis associated with cat scratch disease--broward and palm beach counties, florida, 1994. 19957531250
neuroretinitis in cat-scratch disease associated with the macular star. 19947528909
cat scratch disease. 19947528453
strategy to detect and identify bartonella species in routine clinical laboratory yields bartonella henselae from human immunodeficiency virus-positive patient and unique bartonella strain from his cat.we wished to develop a cost-effective, rapid strategy to detect and identify bartonella species in the clinical laboratory and to determine the prevalence of bartonella infection in the houston veteran population. bartonella colonies were identified by colony morphology, gram stain, rapid ana, repetitive extragenic palindromic-pcr (rep-pcr) and whole-cell fatty acid (cfa) analysis, and these methods were compared for their usefulness. a new test order for "rochalimaea culture" (the genus bartone ...19957559957
prevalence of bartonella henselae antibodies in pet cats throughout regions of north america.cat exposure has been directly associated with the development of human bartonella henselae infections, resulting in cat-scratch disease, bacillary angiomatosis, or bacteremia. the prevalence of serum antibody titers to b. henselae was determined for selected pet cats from 33 geographic locations throughout the united states and several areas in western canada. seroprevalences paralleled increasing climatic warmth (p < .02) and annual precipitation (p < .03). these warm, humid areas with the hig ...19957561200
cat scratch disease. 19947527210
fever, back pain and pleural effusion in a four-year-old boy. 19947526327
rochalimaea species stimulate human endothelial cell proliferation and migration in vitro.rochalimaea henselae and r. quintana are associated clinically with proliferative neovascular lesions. the effect of rochalimaea species on human umbilical vein endothelial cell (huvec) proliferation and migration was evaluated in vitro. cocultivation of rochalimaea organisms with huvecs resulted in enhanced huvec proliferation. fibroblast proliferation was unaffected by r. henselae. huvecs were also stimulated to migrate by rochalimaea. when r. henselae organisms were disrupted and subjected to ...19947523553
intra-abdominal mass associated with gastrointestinal hemorrhage: a new manifestation of bacillary angiomatosis.bacillary angiomatosis is a recently described vascular proliferative lesion that occurs most commonly in individuals infected with human immunodeficiency virus. cutaneous lesions are the most frequently described manifestations of bacillary angiomatosis. however, as culture techniques and disease recognition have improved, additional manifestations have been identified in human immunodeficiency virus-infected individuals, including bacillary peliosis hepatis and isolated bacteremia. two species ...19957498668
persistent generalized lymphadenopathy and non-hodgkin's lymphoma in aids: association with rochalimaea henselae infection.cat scratch disease, which is caused by infection with rochalimaea henselae, is often manifested as lymphadenopathy. r. henselae has also been isolated from human immunodeficiency virus (hiv)-positive patients with bacillary angiomatosis. in order to determine the frequency of r. henselae-reactive antibodies in hiv-positive patients with persistent generalized lymphadenopathy (pgl) or non-hodgkin's lymphoma (nhl), we tested a total of 124 hiv-positive patients for r. henselae-reactive immunoglob ...19947496912
bartonella henselae prevalence in domestic cats in california: risk factors and association between bacteremia and antibody titers.the isolation of bartonella henselae, the agent of cat scratch disease, from the blood of naturally infected domestic cats and the demonstration that cats remain bacteremic for several months suggest that cats play a major role as a reservoir for this bacterium. a convenience sample of 205 cats from northern california was selected between 1992 and 1994 to evaluate the b. henselae antibody and bacteremia prevalences and to determine the risk factors and associations between bacteremia and antibo ...19957494043
characterization of a 17-kilodalton antigen of bartonella henselae reactive with sera from patients with cat scratch disease.a library of bartonella (rochalimaea) henselae dna was constructed in the cloning vector lambda zapii and screened for expression of antigenic proteins by using a pool of sera from patients who had been diagnosed with cat scratch disease (csd) and had antibodies to bartonella spp., as determined by indirect fluorescent-antibody (ifa) assay. ten immunoreactive phages were subcloned as recombinant plasmids by in vivo excision. all 10 recombinants expressed a protein of approximately 17 kda when th ...19957494028
cat-scratch disease in hawaii: etiology and seroepidemiology.to study the etiology and seroepidemiology of cat-scratch disease (csd) in hawaii.19957608806
genomic fingerprinting of bartonella species by repetitive element pcr for distinguishing species and isolates.repetitive-element pcr (rep-pcr) with primers based on repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric) repeated dna sequences was used for genomic finger-printing of bartonella species. this technique was applied by using either extracted genomic dna or preparations of whole bacterial cells directly. pcr fingerprints with either the rep-based primers (rep-pcr) or primers based on the eric repeat (eric-pcr) revealed species-specific band patterns ...19957615711
nucleotide sequence and comparison of the 5s ribosomal rna genes of rochalimaea henselae, r.quintana and brucella abortus. 19937685083
rochalimaea henselae causes bacillary angiomatosis and peliosis hepatis.recent studies have demonstrated that a newly described agent of persistent bacteremia, rochalimaea henselae, and the agent of bacillary angiomatosis are both closely related to rochalimaea quintana. bacillary peliosis hepatis seemed likely to have the same etiologic agent as bacillary angiomatosis. we sought these pathologic changes in patients from whom r henselae was cultivated.19921546924
identification of the rochalimaea henselae 16s rrna sequence in the liver of a french patient with bacillary peliosis hepatis. 19921385550
bacillary angiomatosis.the acquired immunodeficiency syndrome and other causes of immunosuppression have ushered in a variety of opportunistic infections. one of these is bacillary angiomatosis, a vasoproliferative lesion whose principal causative agent is rochalimaea henselae. bacillary angiomatosis, while preponderantly a cutaneous affliction, can be systemic, including involvement of the head and neck mucous membranes. molecular technology and epidemiologic studies used to identify the bacterial agent of bacillary ...19957543738
use of bartonella antigens for serologic diagnosis of cat-scratch disease at a national referral center.bartonella henselae (formerly the genus rochalimaea) has recently been isolated from patients with cat-scratch disease and their cats, and since september 1992 the centers for disease control and prevention has offered an indirect fluorescent antibody assay for bartonella-specific antibody.19957542443
evaluation of the automated bact-alert system for pediatric blood culturing.the organon teknika bact/alert (organon teknika, durham, nc), using the pedi-bact 20 ml aerobic bottle (bpbcs) was compared to the wampole isolator (wi) 1.5 microbial tube (wampole laboratories, cranbury, nj), for detection and recovery of pediatric pathogens. the bpbcs continuously monitors culture bottles for changes in co2 concentrations, while wi cultures are examined twice daily for appearance of colonial growth on agar media. of 5,175 paired blood cultures, 383 pathogens were recovered fro ...19957872254
identification and characterization of a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis.a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. the gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kda in m. a. paratuberculosis. the clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative se ...19947921248
[cat scratch disease].cat scratch disease (csd) is usually manifested in children and young adults as a benign, chronic self-limited lymphadenopathy. however, various atypical presentations have been described, especially in immunocompromised hosts. 1 of 5 cases of csd diagnosed here during 1985-1993 is presented. in 3 cases the diagnosis was supported by lymph node biopsy in which the organisms were visualized by warthin-starry silver impregnation staining. recent publications indicate that afipia felis and rochalim ...19947927015
pathological case of the month. systemic cat-scratch disease. 19957540915
[cat-scratch disease and bacillary angiomatosis. an old and a new infectious disease with common etiology?].a review of cat-scratch disease (csd) and bacillary angiomatosis (ba) is presented on the basis of published articles. two newly identified bacteria--rochalimaea henselae and afipia felis--have been isolated from patients with csd. preliminary investigations seem to indicate that a. felis is an uncommon cause of the disease. csd may appear as a local suppurative lymphadenopathy or a systemic infection. ba is caused by rochalimaea species and may appear as cutaneous, mucous or visceral angiomas o ...19947998352
detection of rochalimaea henselae dna in specimens from cat scratch disease patients by pcr.a pcr assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of dna from the rickettsia-like organisms rochalimaea henselae and r. quintana. internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two rochalimaea species. dnas from 12 different isolates of r. henselae were amplified with the pcr primers, and the resulting 414-bp pcr product hybridized only with the r. henselae-specific probe. dnas from four dif ...19948027347
cat scratch disease in two children presenting with fever of unknown origin: imaging features and association with a new causative agent, rochalimaea henselae.to report the clinical course, imaging findings, and method of diagnosis of two patients with systemic manifestations of cat scratch disease, presenting with fever of unknown origin.19957536916
intraerythrocytic presence of bartonella henselae.recent reports in the medical literature emphasize the risk of zoonotic disease and the high degree of prevalence of asymptomatic feline infection with bartonella (rochalimaea) henselae. while investigating bartonella bacteremia in cats, we used transmission electron microscopy to demonstrate b. henselae in the erythrocytes of persistently bacteremic cats.19957544366
[perinaud's oculoglandular syndrome as a manifestation of cat-scratch disease].the first danish case of parinaud's oculoglandular syndrome, a manifestation of cat-scratch disease, is reported in a 35-year-old man presenting with an enlarged preauricular lymph node and an ipsilateral conjunctival granuloma. surgical removal of the granulomatous lesion was followed by rapid healing. the diagnosis was verified by demonstrating a high antibody tire against rochalimaea (bartonella) henselae. on subsequent questioning the man gave a history of acquiring a kitten six weeks before ...19957544512
prevalence of antibodies to rochalimaea species (cat-scratch disease agent) in cats. 19957544936
pulmonary manifestations of cat-scratch disease; a case report and review of the literature. 19957545277
bartonella henselae: etiology of pulmonary nodules in a patient with depressed cell-mediated immunity.we describe an immunocompromised renal transplantation patient with opportunistic lung infection due to bartonella henselae (formerly rochalimaea henselae) and provide evidence suggesting transmission from a pet cat. computed tomographic scans of the chest and lung biopsies provided material for diagnosis. the etiology was established by polymerase chain reaction and sequencing of a 16s ribosomal dna segment from infected lung tissue. histopathologic and serological evidence supported the molecu ...19957548500
neurologic complications of bartonella henselae infection.bartonella henselae can cause cat scratch disease and bacillary angiomatosis, a multisystem disorder seen primarily in patients with the acquired immunodeficiency syndrome. both of these diseases are associated with neurologic complications, particularly encephalopathy. b. henselae may also cause bacteremia and endocarditis, and has been associated with aseptic meningitis and with dementia in patients also infected with the human immunodeficiency virus. recent advances in identification of this ...19957551113
rochalimaea henselae organisms possess an elevated capacity of binding to peripheral blood lymphocytes from patients with cat scratch disease.cat scratch disease (csd) is a clinical condition whose aetiological agent, according to recent findings, is of bacterial origin. two gram-negative bacteria are invoked as causative agents of csd, namely afipia felis and rochalimaea henselae. in this paper, five patients with suspected csd were studied in terms of binding capacity of a. felis and r. henselae to their own peripheral blood lymphocytes (pbl). this parameter was correlated with serum antibody titres to both a. felis and r. henselae, ...19948152395
cat-scratch encephalopathy: a cause of status epilepticus and coma in a healthy young adult. 19957529901
cutaneous manifestations of opportunistic infections in patients infected with human immunodeficiency virus.bacillary angiomatosis (ba) presents most commonly as a cutaneous disease and is caused by two organisms. bartonella (rochalimaea) henselae and bartonella (rochalimaea) quintana. biopsy confirmation of cutaneous ba is essential because lesions can mimic nodular kaposi's sarcoma in appearance. although the vast majority of human immunodeficiency virus (hiv)-infected patients with ba have cd4 lymphocyte counts of less than 100 cells per mm3, the disease responds well to antimicrobial therapy. stap ...19957553576
morphologically variable bacilli of cat scratch disease are identified by immunocytochemical labeling with antibodies to rochalimaea henselae.the identification of the causative organisms of cat scratch disease (csd) has been elusive. the demonstration of warthin-starry stain-positive pleomorphic bacilli in lymph nodes of patients with csd and recent serologic and epidemiologic data suggest an etiologic role of rochalimaea henselae in csd. the authors studied lymph node biopsy specimens of 46 patients with illnesses clinically consistent with csd and found pleomorphic bacilli in 15 (33%). the organisms were labeled by polyclonal rabbi ...19948178768
proposals to unify the genera bartonella and rochalimaea, with descriptions of bartonella quintana comb. nov., bartonella vinsonii comb. nov., bartonella henselae comb. nov., and bartonella elizabethae comb. nov., and to remove the family bartonellaceae from the order rickettsiales.dna hybridization data (hydroxyapatite method, 50 to 70 degrees c) indicate that rickettsia prowazekii, the type species of the type genus of the family rickettsiaceae, is substantially less closely related to rochalimaea species than was previously thought. the levels of relatedness of rickettsia prowazekii to rochalimaea species and to bartonella bacilliformis under optimal conditions for dna reassociation were 0 to 14%, with 25.5% or greater divergence in related sequences. when stringent rea ...19938240958
ehrlichia chaffeensis and rochalimaea antibodies in kawasaki disease.sera from 38 patients with kawasaki disease were tested for immunofluorescent antibodies to ehrlichia chaffeensis, rochalimaea henselae, and r. quintana oklahoma. only 2.5% of the patients tested positive for e. chaffeensis, and 5% were positive for r. henselae and r. quintana oklahoma. our data suggest that ehrlichia and rochalimaea spp. do not play a unique role in the etiology of kawasaki disease.19938263201
seroepidemiological survey of bartonella (rochalimaea) henselae in domestic cats in japan.a total of 199 domestic cat serum samples from 3 geographical areas (northeastern, central and southwestern) of japan collected between 1992 and 1994 were examined for serum antibody against bartonella henselae using an immunofluorescent assay. the antibody prevalence was 15.1% (30/199). a significant difference in the prevalence of b. henselae antibody was observed between the northeastern area (6.3%:3/48) and the central area (22.0:13/59) in japan. there was no significant difference between t ...19957565174
bacillary angiomatosis in patients with aids: multiorgan imaging findings.to describe the varied clinical manifestations and imaging findings encountered in bacillary angiomatosis, an infectious complication of the acquired immunodeficiency syndrome (aids).19957568856
antimicrobial susceptibility of rochalimaea quintana, rochalimaea vinsonii, and the newly recognized rochalimaea henselae.the susceptibility of an isolate of rochalimaea quintana, rochalimaea vinsonii and the newly recognized rochalimaea henselae to antimicrobial agents was determined by dilution in mueller-hinton agar supplemented with 5% sheep blood. two inocula of 10(2) and 10(4) cfu were used and tests were read after incubation at 5 and 7 days at 37 degrees c in an atmosphere enriched to 5% carbon dioxide. each isolate was susceptible to amoxycillin, third-generation cephalosporins, tetracyclines, macrolides a ...19938288500
encephalitis associated with cat scratch disease--broward and palm beach counties, florida, 1994.on august 14, 1994, the broward county public health unit of the florida department of health and rehabilitative services was notified of three children from pompano beach who were hospitalized with encephalitis attributed to cat scratch disease (csd). all three children (aged 5, 6, and 11 years) were previously healthy and had no histories of seizure disorders or diagnoses of csd. this report summarizes the investigation of these cases.19947527121
[bacillary peliosis in aids. anatomo-clinical study of 2 cases].the authors report two cases of peliosis hepatis, occurring in patients with aids, who presented a persistent fever and an hepatomegaly. the liver biopsies showed areas of peliosis, where bacilli were observed by warthin-starry stain. in one case, techniques of molecular biology allowed the identification of rochalimaea henselae, pathogen involved in bacillary angiomatosis. this rickettsia has been newly recognized in the united-states, where 17 cases of bacillary peliosis have been published in ...19938311862
bacteriophage-like particle of rochalimaea henselae.an extracellular particle approximately 40 nm in diameter was detected in culture supernatant from the fastidious bacterium rochalimaea henselae. this particle has at least three associated proteins and contains 14 kbp linear dna segments that are heterogeneous in sequence. the 14 kbp dna was also present in r. henselae cells as an extrachromosomal element for all 14 strains tested. despite attempts to induce lysis of r. henselae, plaque formation was not observed. a similar particle, also conta ...19947527118
[bacillary angiomatosis. a pseudoneoplastic infection in aids patients].bacillary angiomatosis is a newly recognized pseudoneoplastic vascular disease seen in the setting of human immunodeficiency virus (hiv) infection. the disease is characterized by a cutaneous infection with reddish papules or nodules that are similar to pyogenic granulomas or kaposi's sarcoma in clinical appearance. it is caused by the mildly gram-negative bacillus rochalimaea henselae, which can be identified in tissue sections by means of warthin-starry stain. the historical, clinical, histopa ...19938335458
bartonella infections. evolution from the esoteric. 19957572806
detection of bartonella (rochalimaea) henselae bacteremia using bact/alert blood culture system.bartonella henselae was isolated from the blood of five febrile immunosuppressed patients using bact/alert (organon teknika, durham, nc) automated microbial detection system. an immunofluorescence assay (using 1:1000 dilutions) was used to confirm identification of fastidious, pleomorphic, non-gram staining, argyrophilic bacilli displaying rachety motility that had been presumptively identified as bartonella spp. the practicality of identification of bartonella henselae using goat antisera for u ...19957572813
detection of rochalimaea henselae in cat-scratch disease skin test antigens.one of the diagnostic criteria for cat-scratch disease is a positive delayed-type hypersensitivity skin test reaction to an antigen prepared from purulent material aspirated from a cat-scratch disease-involved lymph node. polymerase chain reaction coupled with dna sequence analysis was used to identify organisms found in these skin test antigen preparations that may be responsible for eliciting this response. two independent sources of cat-scratch disease skin test antigens yielded dna sequences ...19938376816
bartonella henselae and bartonella quintana adherence to and entry into cultured human epithelial cells.bartonella henselae expresses pili phenotypically similar to type 4 pili. b. henselae pilus expression undergoes phase variation with multiple passages. low-passage-number, piliated b. henselae adhered to and invaded hep-2 cells to a greater extent than did multiply passaged b. henselae with reduced pilus expression. pili may be a pathogenic determinant for bartonella species.19957591104
syndrome of rochalimaea henselae adenitis suggesting cat scratch disease.to describe a clinical syndrome of cat scratch disease caused by rochalimaea henselae, including methods for isolation of the organism from tissue and for identification.19938430978
endocarditis caused by rochalimaea quintana in a patient infected with human immunodeficiency virus.rochalimaea quintana and rochalimaea henselae are closely related, fastidious, gram-negative rickettsiae. thus far, the spectrum of human rochalimaea sp. infections has not included endocarditis. we describe a 50-year-old human immunodeficiency virus-positive man who developed endocarditis caused by r. quintana. dna relatedness studies, which compared our patient's blood culture isolate with known rochalimaea species, identified the organism as r. quintana. our report expands the spectrum of roc ...19938458964
diagnostic and therapeutic problems due to cat scratch disease. 19957543544
[recognition and identification of unknown infectious agents].the recognition and the identification of previously unrecognized infectious agents require a multidisciplinary approach to specify the nosologic entity of the disease and the epidemiological data, especially the modes of transmission and the risk factors, as well as to discover the microorganism in the laboratory. in the past 20 years, significant breakthroughs have been achieved in cellular cultures (growth factors), in immunology (monoclonal antibodies), and moreover in molecular biology, whi ...19947939294
ocular cat scratch disease.cat scratch disease (csd) is infectious and can cause a multitude of systemic diseases in addition to ocular csd. rochalimaea henselae has been identified as the causative agent for ophthalmic manifestations of cat scratch disease. a noninvasive method of making a positive diagnosis of ocular csd was recently developed.19957594915
ophthalmic manifestations of rochalimaea species.rochalimaea henselae and r. quintana belong to the order rickettsiales and are thought to be responsible for trench fever, bacillary angiomatosis, and cat scratch disease. we recently examined four patients with intraocular inflammation of unknown origin. each patient had either unilateral or bilateral moderate loss of visual acuity ranging from 20/25 to counting fingers. bilateral intraocular inflammation manifested by anterior and posterior segment cells, retinal lesions, macular exudate, and ...19948053459
[hepatic peliosis, a still current disease]. 19957590002
[vascular tumors of the skin and soft tissue. overview of newly characterized entities and variants].this review summarizes vascular tumours of skin and soft tissues that have been characterised in recent years. although most of them are very rare, knowledge about their reproducible clinicopathological features is important to avoid diagnostic pitfalls. these lesions include: bacillary angiomatosis, a vasoproliferative, pseudoneoplastic infection of immunocompromised patients caused by rochalimaea henselae; tufted angioma, a variant of lobular capillary haemangioma characterised by a "cannon-ba ...19947824435
cat-scratch disease: single or multiple causative microorganisms? 19958559644
polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from rochalimaea species for subtyping.restriction endonuclease analysis of a polymerase chain reaction-amplified dna fragment which included the spacer region between the genes coding for 16s and 23s rrnas and a portion of the gene coding for 23s rrna (spacer + 23s) was done on 10 previously characterized clinical isolates of rochalimaea henselae, one clinical isolate of rochalimaea quintana, and the type strains of r. henselae, r. quintana, rochalimaea vinsonii, and bartonella bacilliformis. brucella abortus dna was not amplified b ...19938102375
bacillary angiomatosis. clinical and histologic features, diagnosis, and treatment.bacillary angiomatosis is a relatively new infection affecting primarily patients with human immunodeficiency virus or others with impaired host defenses. it presents most commonly with multiple red skin lesions, but visceral involvement may also occur, including involvement of the liver and spleen. because of the dermatologic manifestations, bacillary angiomatosis may be mistaken for kaposi's sarcoma. the diagnosis is made by identification of the characteristic histologic findings or genetic a ...19948122945
rochalimaea henselae infections: newly recognized zoonoses transmitted by domestic cats. 19948144389
bacillary angiomatosis of the spleen.bacillary angiomatosis is a recently described vasoproliferative lesion associated with infection by a newly characterized rickettsial organism, rochalimaea henselae. most previous reports have described skin lesions in immunocompromised patients infected with human immunodeficiency virus. this is the first case report detailing the features of bacillary angiomatosis of the spleen occurring in a patient undergoing cytotoxic chemotherapy for disseminated ovarian carcinoma.19938165007
cat-scratch disease mimicking rhabdomyosarcoma.a 12-year-old boy who had injured his elbow presented with a lump in the epitrochlear area. originally thought to be a hematoma, then later myositis ossificans, this mass was actually a lymph node with large, reactive germinal centers. although stains for acid-fast bacilli, fungal organisms, bacteria, and cat-scratch disease were negative, serological testing for detection of rochalimaea henselae organisms found evidence of cat-scratch disease. we would like to remind orthopaedic surgeons to con ...19938265222
rochalimaea antibodies in hiv-associated neurologic disease.rochalimaea henselae, a recently described pathogen thought to cause syndromes as varied as bacillary angiomatosis, parenchymal bacillary peliosis, fever with bacteremia, and cat-scratch disease, is associated with cns diseases including cerebral and retinal bacillary angiomatosis, as well as cat-scratch-related encephalitis, myelitis, cerebral arteritis, and retinitis. we used a newly developed enzyme immunoassay and the polymerase chain reaction to investigate the association of r henselae inf ...19948035937
epidemiologic observations on infection with rochalimaea species among cats living in baltimore, md.cats from several sources in baltimore, md, were tested for seropositivity to rochalimaea henselae and r quintana. co-infection with toxoplasma gondii or feline immunodeficiency virus was assessed as a risk factor for infection with rochalimaea spp. of 592 cats tested, 87 (14.7%) were seropositive for one or both rochalimaea spp, although titers to r henselae were significantly higher than those to r quintana. prevalence of seropositivity increased significantly with cat age and weight and was a ...19948063598
disseminated cat-scratch disease: detection of rochalimaea henselae in affected tissue.an immunocompetent 9-year-old boy with disseminated cat scratch disease involving spleen, cervical and abdominal lymph nodes, skull, and one clavicle is reported. antibodies to rochalimaea quintana and r. henselae were detected, at increasing, then decreasing concentration. dna extracted from the biopsied skull lesion was amplified by polymerase chain reaction and hybridized with species-specific oligonucleotides proving the presence of r. henselae in affected tissue. our findings suggest that r ...19948313920
cat scratch disease and rochalimaea henselae. 19938336627
development and evaluation of a blood-free medium for determining growth curves and optimizing growth of rochalimaea henselae.two strains of rochalimaea henselae were used to optimize a blood-free growth medium. seven agar bases, four broths, and combinations of eight supplements were evaluated. acceptable growth was achieved in media containing fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% fildes solution and 250 micrograms of hemin per ml. r. henselae utilized hemin in concentrations six times that utilized by rochalimaea quintana. erythrocyte membrane ...19938349767
cat scratch disease and bacillary angiomatosis: aetiological agents and the link with aids.the recent characterisation of a new species of the genus rochalimaea is the culmination of a ten year search for the identity of the agent of bacillary angiomatosis, a condition that is increasingly recognised in association with aids. this work has also implicated rochalimaea henselae in the aetiology of cat scratch disease contact with cats is a risk factor for both conditions. this report describes both diseases and reviews progress toward understanding their microbiology. r. quintana is als ...19937693166
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