| studies on the development of rhodnius prolixus and the effects of its symbiote nocardia rhodnii. | | 1974 | 4208144 |
| the use of artificial diets to determine some of the effects of nocardia rhodnii on the development of rhodnius prolixus. | | 1968 | 5649234 |
| [the microbial transformation of phenanthrene and anthracene]. | the transformation of phenanthrene and anthracene by rhodococcus rhodnii 135, pseudomonas fluorescens 26k, and arthrobacter sp. k3 is studied. twenty-one intermediates of phenanthrene and anthracene transformation are identified by hplc, mass spectrometry, and nmr spectroscopy. p. fluorescens 26k and arthrobacter sp. k3 are found to produce a wide range of intermediates, whereas r. rhodnii 135 oxidizes phenanthrene, resulting in the formation of a sole product, 3-hydroxyphenanthrene. putative tr ... | 2005 | 16119849 |
| draft genome sequence of rhodococcus rhodnii strain lmg5362, a symbiont of rhodnius prolixus (hemiptera, reduviidae, triatominae), the principle vector of trypanosoma cruzi. | we report the 4,385,577-bp high-quality draft assembly of the bacterial symbiont rhodococcus rhodnii strain lmg5362, isolated from the gut of rhodnius prolixus (hemiptera, reduviidae, triatominae), the principle vector of the protozoan trypanosoma cruzi, the etiological agent of chagas disease. this sequence might provide useful information for subsequent studies of the symbiotic relationship between rd. prolixus and rc. rhodnii, while also providing a starting point for the development of biote ... | 2013 | 23788540 |
| rhodococcus aerolatus sp. nov., isolated from subarctic rainwater. | a gram-stain-positive, rod-shaped and non-motile strain, designated pamc 27367(t), was isolated from rainwater collected on the bering sea. analysis of the 16s rrna gene sequence of the strain showed an affiliation with the genus rhodococcus. phylogenetic analyses revealed that strain pamc 27367(t) formed a robust clade with the type strains of rhodococcus rhodnii, rhodococcus aetherivorans and rhodococcus ruber with 16s rrna gene sequence similarities of 96.3 %, 95.8 % and 95.5 %, respectively. ... | 2015 | 25385992 |
| genetically modifying the insect gut microbiota to control chagas disease vectors through systemic rnai. | technologies based on rna interference may be used for insect control. sustainable strategies are needed to control vectors of chagas disease such as rhodnius prolixus. the insect microbiota can be modified to deliver molecules to the gut. here, escherichia coli ht115(de3) expressing dsrna for the rhodnius heme-binding protein (rhbp) and for catalase (cat) were fed to nymphs and adult triatomine stages. rhbp is an egg protein and cat is an antioxidant enzyme expressed in all tissues by all devel ... | 2015 | 25675102 |
| impact of trypanosoma cruzi on antimicrobial peptide gene expression and activity in the fat body and midgut of rhodnius prolixus. | rhodnius prolixus is a major vector of trypanosoma cruzi, the causative agent of chagas disease in latin america. in natural habitats, these insects are in contact with a variety of bacteria, fungi, virus and parasites that they acquire from both their environments and the blood of their hosts. microorganism ingestion may trigger the synthesis of humoral immune factors, including antimicrobial peptides (amps). the objective of this study was to compare the expression levels of amps (defensins an ... | 2016 | 26931761 |
| modeling horizontal gene transfer (hgt) in the gut of the chagas disease vector rhodnius prolixus. | abstract: background: paratransgenesis is an approach to reducing arthropod vector competence using genetically modified symbionts. when applied to control of chagas disease, the symbiont bacterium rhodococcus rhodnii, resident in the gut lumen of the triatomine vector rhodnius prolixus (hemiptera: reduviidae), is transformed to export cecropin a, an insect immune peptide. cecropin a is active against trypanosoma cruzi, the causative agent of chagas disease.while proof of concept has been achiev ... | 2011 | 21569540 |
| trypanosoma cruzi: synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to t. cruzi and potential bacterial hosts. | the parasite trypanasoma cruzi is responsible for chagas disease and its triatomine vector, rhodnius prolixus, has a symbiotic relationship with the soil bacterium, rhodococcus rhodnii. r. rhodnii that was previously genetically engineered to produce the anti-microbial peptide, cecropin a was co-infected with t. cruzi into r. prolixus resulting in clearance of the infectious t. cruzi in 65% of the vectors. similar anti-microbial peptides have been isolated elsewhere and were studied for differen ... | 2010 | 20206169 |
| rhodococcus triatomae sp. nov., isolated from a blood-sucking bug. | two bacterial isolates, strains immib riv-085(t) and immib riv-095, isolated from a blood-sucking bug of the genus triatoma, were characterized by phenotypic and molecular taxonomic methods. chemotaxonomic investigations revealed the presence of cell-wall chemotype iv and mycolic acids consistent with the genus rhodococcus. comparative 16s rrna gene sequencing showed that the two isolates are genealogically highly related (100% sequence similarity) and constitute a new subline within the genus r ... | 2005 | 16014484 |
| synthesis of thiamine and folic acid by nocardia rhodnii, the micro-symbiont of rhodnius prolixus. | | 1960 | 13711570 |
| transformation of rhodococcus rhodnii, a symbiont of the chagas disease vector rhodnius prolixus, with integrative elements of the l1 mycobacteriophage. | elimination of vector populations through the use of insecticides is the principal means of controlling chagas disease. because of the limitations of insecticide use, we have been developing a new potential method of control, to be used in conjunction with insecticide programs, a method which utilizes genetically modified symbiotic bacteria. these transformed bacteria can express anti-parasitic agents in the gut of the bug where the trypanosomes also are found. previous studies have shown that i ... | 2003 | 12809804 |
| development of symbionts in triatomine bugs and the effects of infections with trypanosomatids. | in the intestinal tract of fifth instars of the hematophagous reduviid bugs rhodnius prolixus and triatoma infestans blood ingestion induced an initial decrease of the concentration of the respective symbiotic bacteria rhodococcus rhodnii and nocardia sp. and then within 10 days a 15- or 18-fold increase of the total population/bug to about 0.8 x 10(9) colony-forming units in r. prolixus and 1.8 x 10(9) colony-forming units in t. infestans. about 95-99% of the total populations of both symbionts ... | 2002 | 11971650 |
| expression of a functional antibody fragment in the gut of rhodnius prolixus via transgenic bacterial symbiont rhodococcus rhodnii. | expression within insects of foreign antiparasitic gene products via microbial symbionts could be used to prevent transmission of vector-borne pathogens to vertebrate hosts. genetically transformed symbiotic bacteria rhodococcus rhodnii expressed functional antibody fragments (rdb3 encoding murine v(h)/k which binds progesterone) that were exported into the gut lumen of the triatomine bug rhodnius prolixus (hemiptera: reduviidae), a vector of chagas disease. transgenic symbionts were maintained ... | 1999 | 10484156 |
| isolation and characterization of catechol 1,2-dioxygenases from rhodococcus rhodnii strain 135 and rhodococcus rhodochrous strain 89: comparison with analogous enzymes of the ordinary and modified ortho-cleavage pathways. | catechol 1,2-dioxygenases of the ordinary ortho-cleavage pathway have been isolated from strains rhodococcus rhodnii 135 and rhodococcus rhodochrous 89 grown on phenol as the sole source of carbon and energy. the activities of the catechol 1,2-dioxygenases with 3- and 4-methylpyrocatechols were 1.3-1.5 times higher than those with pyrocatechol. the rate of oxidation of 3-chloropyrocatechol catalyzed by both enzymes was 20% of the rate of oxidation of unsubstituted pyrocatechol. the enzymes are h ... | 1999 | 10424908 |
| a chemotaxonomic study of the lipoglycans of rhodococcus rhodnii n445 (ncimb 11279). | rhodococcus rhodnii n445 was investigated for the presence of macroamphiphilic lipoglycan. purification of a hot phenol-water extract by hydrophobic interaction chromatography allowed the resolution of three lipoglycan fractions. the two main preparations contained lipoglycans with carbohydrate compositions consistent with the presence of lipoarabinomannan and lipomannan, whilst the minor fraction appeared to contain a mixture of these two lipoglycans. the fatty acid composition of the lipoglyca ... | 1996 | 8946691 |
| transformation of an insect symbiont and expression of a foreign gene in the chagas' disease vector rhodnius prolixus. | a shuttle plasmid was developed that is capable of replicating both in escherichia coli and in rhodococcus rhodnii, a bacterial symbiont of the chagas' disease vector rhodnius prolixus. we have been able to transform r. rhodnii with this plasmid, infect aposymbiotic r. prolixus with the transformed symbionts, select with the antibiotic thiostrepton, and re-isolate genetically altered symbionts from the insects following successive molts. symbiotic bacteria are potentially valuable as vehicles fo ... | 1992 | 1539755 |
| recombinant arthrobacter β-1, 3-glucanase as a potential effector molecule for paratransgenic control of chagas disease. | chagas disease is most often transmitted to humans by trypanosoma cruzi infected triatomine bugs, and remains a significant cause of morbidity and mortality in central and south america. control of chagas disease has relied mainly on vector eradication. however, development of insect resistance has prompted us to develop a paratransgenic strategy to control vectorial transmission of t. cruzi. here, the potential role of recombinant endoglucanases as anti-trypanosomal agents for paratransgenic ap ... | 2013 | 23497594 |