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5-keto-d-fructose reductase from gluconobacter cerinus-1. 1975236428
aldohexose dehydrogenase from gluconobacter cerinus-1. 1975236431
an ornithine-containing lipid isolated from gluconobacter cerinus.the three ornithine-containing lipids of gluconobacter cerinus were isolated from each other. one of the three lipids was postulated as nalpha-3-hydroxypalmitoylornithine, to the fatty acid moiety of which 2-hydroxy fatty acid is linked by an ester linkage. the 2-hydroxy acid was possibly cis-11, 12-methylene-2-hydroxyoctadecanoate. such an ornithine-containing lipid was found to be distributed in other acetic acid bacteria.1976990302
the restriction endonuclease gceini of gluconobacter cerinus ifo 3260, an isoschizomer of bamhi, has a monomeric structure. 19901368602
5-keto-d-fructose: formation and utilization in the course of d-fructose as similation by gluconabacter cerinus.the accumulation of 5-keto-d-fructose (5kf) by gluconobacter cerinus grown on d-fructose in unbuffered medium was shown to be optimal at ph 4.0 after cell growth ceased. during the exponential phase of growth or at neutral ph after the onset of the stationary phase, 5kf production continued but did not accumulate because of its rapid reutilization by reduction to d-fructose. the extent of isotope incorporation into c5 of ribonucleic acid ribose when cells were grown in the presence of specifical ...19744151451
5-keto-d-fructose. iv. a specific reduced nicotinamide adenine dinucleotide phosphate-linked reductase from gluconobacter cerinus. 19664379259
purification and properties of a nicotinamide adenine dinucleotide phosphate-linked aldohexose dehydrogeanse from gluconobacter cerinus. 19684384672
metabolic consequences of a block in the synthesis of 5-keto-d-fructose in a mutant of gluconobacter cerinus.a mutant of gluconobacter cerinus var. ammoniacus, ifo 3267, has been isolated which is deficient with respect to fructose 5-dehydrogenase, the enzyme catalyzing the oxidation of d-fructose to 5-keto-d-fructose (5 kf). growth of this mutant on fructose as the sole carbon source was impaired unless the culture medium was supplemented with 5 kf. significant randomization of the 1 and 6 positions of fructose has been reported previously for the wild-type organism during growth on this ketohexose. t ...19744853173
solution structure of 5-keto-d-fructose: relevance to the specificity of hexose kinases.5-keto-d-fructose (5kf) is isolated from cultures of gluconobacter cerinus growing on d-fructose as the sole carbon source. 5kf is a substrate for hexokinase, fructokinase, and several polyol dehydrogenases. 1h and 13c nuclear magnetic resonance studies show that 5kf exists in different forms in anhydrous dimethyl-d6 sulfoxide and d2o. in dimethyl-d6 sulfoxide, 5kf exists as a spirane dimer with linked furanose and pyranose rings, similar to the structure reported for crystalline 5kf [hassen, l. ...19827059583
the phylogeny of acetic acid bacteria based on the partial sequences of 16s ribosomal rna: the elevation of the subgenus gluconoacetobacter to the generic level.thirty-six strains of acetic acid bacteria classified in the genera acetobacter, gluconobacter, and acidomonas were examined for their partial base sequences in positions 1220 through 1375, 156 bases, of 16s rrna. the strains of the q10-equipped gluconobacter species examined were divided into two subgroups, which included the type strains of gluconobacter oxydans, the type species of the genus gluconobacter, and of a second species, gluconobacter cerinus, respectively. the base differences numb ...19979301103
gluconobacter asaii mason and claus 1989 is a junior subjective synonym of gluconobacter cerinus yamada and akita 1984.five strains received as gluconobacter cerinus and gluconobacter asaii were examined for dna base composition, dna-dna similarity, 16s rrna gene sequences and phenotypic characteristics, including acid production from ethanol, growth on l-arabitol and meso-ribitol and requirement for nicotinic acid. the five strains showed dna base compositions ranging from 54 to 56 mol% g+c. g. cerinus ifo 3267t and iam 1832 and g. asaii ifo 3276t and ifo 3275 showed high levels of dna-dna similarity (70-100%) ...200212361267
identification of acetic acid bacteria isolated from indonesian sources, especially of isolates classified in the genus gluconobacter.sixty-four strains of acetic acid bacteria were isolated from indonesian sources such as fruits, flowers, and fermented foods by the enrichment culture at ph 3.5. forty-five strains were routinely identified as acetobacter strains because of their oxidation of acetate and lactate to carbon dioxide and water and their q-9 isoprenolog, corresponding to 70% of all the 64 acetic acid bacteria isolated. eight isolates were identified as gluconacetobacter strains because of their oxidation of acetate ...199912501398
5-keto-d-fructose. i. chemical characterization and analytical determination of the dicarbonylhexose produced by gluconobacter cerinus. 196514304828
5-keto-d-fructose. ii. patterns of formation and of associated dehydrogenase activities in gluconobacter cerinus. 196514304829
identification of strains assigned to the genus gluconobacter asai 1935 based on the sequence and the restriction analyses of the 16s-23s rdna internal transcribed spacer regions.thirteen reference strains, including the type strains of the type species of the genus gluconobacter, gluconobacter oxydans (nbrc 14819t), gluconobacter cerinus (nbrc 3267t), and gluconobacter frateurii (ifo 3264t) were examined for their species identification based on the sequence and the restriction analyses of the 16s-23s rdna internal transcribed spacer (its) regions. a phylogenetic tree constructed by the neighbor-joining method represented three clusters corresponding respectively to the ...200415057706
gluconobacter thailandicus sp. nov., an acetic acid bacterium in the alpha-proteobacteria.four strains of acetic acid bacteria were isolated from flowers collected in thailand. in phylogenetic trees based on 16s rrna gene sequences and 16s-23s rdna internal transcribed spacer (its) region sequences, the four isolates were located in the lineage of the genus gluconobacter and constituted a separate cluster from the known gluconobacter species, gluconobacter oxydans, gluconobacter cerinus, and gluconobacter frateurii. in addition, the isolates were distinguished from the known species ...200415486825
biocontrol of the food-borne pathogens listeria monocytogenes and salmonella enterica serovar poona on fresh-cut apples with naturally occurring bacterial and yeast antagonists.fresh-cut apples contaminated with either listeria monocytogenes or salmonella enterica serovar poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. while most of the antagonists increased the growth of the food-borne pathogens, four of them, including gluconobacter asaii (t1-d1), a candida sp. (t4-e4), discosphaerina fagi (st1-c9), and metschnikowia pulcherrima (t1-e2), proved ef ...200616461659
gluconobacter japonicus sp. nov., an acetic acid bacterium in the alphaproteobacteria.five strains, nbrc 3271(t), nbrc 3272, nbrc 3263, nbrc 3260 and nbrc 3269 were examined genetically, phylogenetically, phenotypically and chemotaxonomically. the dna g+c contents of the five strains were 55.1-56.4 mol%. the five strains had low levels of dna-dna hybridization of 13-51 % to the type strains of gluconobacter frateurii, gluconobacter thailandicus, gluconobacter oxydans, gluconobacter cerinus, gluconobacter albidus and gluconobacter kondonii and formed a cluster that was separate fr ...200919244423
Adsorption induced enzyme denaturation: the role of protein surface in adsorption induced protein denaturation on allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymers.The effects of protein size on adsorption and adsorption-induced denaturation of proteins on copolymers of allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) have been studied. Different responses were observed for the amount of protein adsorbed and denatured on the polymer surface for different proteins (trypsin, alchol dehydrogenase from baker's yeast (YADH), glucose dehydrogenase (GDH) from Gluconobacter cerinus, and alkaline phosphates from calf intestinal mucosa (CIAP). Protei ...201222056082
structural analysis of fructans produced by acetic acid bacteria reveals a relation to hydrocolloid function.some strains of acetic acid bacteria (gluconobacter frateurii tmw 2.767, gluconobacter cerinus dsm 9533t, neoasaia chiangmaiensis nbrc 101099, kozakia baliensis dsm 14400) produce high amounts of fructans, which can be exploited in food applications as previously demonstrated empirically for dough systems. in order to get insight into the structure and functionality of these polymers, we investigated the fructans isolated from these strains with respect to their linkage types and molecular weigh ...201223399151
acetic acid bacteria isolated from grapes of south australian vineyards.acetic acid bacteria (aab) diversity from healthy, mould-infected and rot-affected grapes collected from three vineyards of adelaide hills (south australia) was analyzed by molecular typing and identification methods. nine different aab species were identified from the 624 isolates recovered: four species from gluconobacter genus, two from asaia and one from acetobacter were identified by the analysis of 16s rrna gene and 16s-23s rrna gene internal transcribed spacer. however, the identification ...201424681711
investigation of the instability and low water kefir grain growth during an industrial water kefir fermentation process.a poorly performing industrial water kefir production process consisting of a first fermentation process, a rest period at low temperature, and a second fermentation process was characterized to elucidate the causes of its low water kefir grain growth and instability. the frozen-stored water kefir grain inoculum was thawed and reactivated during three consecutive prefermentations before the water kefir production process was started. freezing and thawing damaged the water kefir grains irreversib ...201728070662
draft genome sequences of gluconobacter cerinus cect 9110 and gluconobacter japonicus cect 8443, acetic acid bacteria isolated from grape must.we report here the draft genome sequences of gluconobacter cerinus strain cect9110 and gluconobacter japonicus cect8443, acetic acid bacteria isolated from grape must. gluconobacter species are well known for their ability to oxidize sugar alcohols into the corresponding acids. our objective was to select strains to oxidize effectively d-glucose.201627365351
elucidation and identification of amino acid containing membrane lipids using liquid chromatography/high-resolution mass spectrometry.intact polar lipids (ipls) are the building blocks of cell membranes, and amino acid containing ipls have been observed to be involved in response to changing environmental conditions in various species of bacteria. high-performance liquid chromatography/mass spectrometry (hplc/ms) has become the primary method for analysis of ipls. many glycerol-free amino acid containing membrane lipids (aa-ipls), which are structurally different than abundant aminophospholipids, have not been characterized us ...201627281845
reclassification of the strains with low g+c contents of dna belonging to the genus gluconobacter asai 1935 (acetobacteraceae).three species, gluconobacter cerinus, g. asaii, and g. frateurii are reported to show lower g+c contents than g. oxydans. isolate 145 also showed a similar g+c content to those of the three species. we try to reclassify the three species. g. frateurii including a type strain, isolate 145, g. cerinus ifo 3262, 3263, 3269, and g. asaii ifo 3265 formed acid from d-arabitol, ribitol, and l-arabitol. dnas from g. cerinus ifo 3262, 3263, 3269, and g. asaii ifo 3265 showed 100-53% sequence similarity w ...199927389333
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