| evaluation of the genus listonella and reassignment of listonella damsela (love et al.) macdonell and colwell to the genus photobacterium as photobacterium damsela comb. nov. with an emended description. | the genus listonella, which was recently described on the basis of 5s rrna sequence data, was found to be of dubious value on the basis of the results of a comparison of a number of taxonomic studies involving members of the vibrionaceae. the available data suggest that 5s rrna sequences may be of limited taxonomic use at the intra- and intergeneric levels, at least for apparently recently evolved groups, such as the vibrionaceae. in this light, we assessed the generic assignment of the species ... | 1991 | 1742198 |
| small-subunit rrna sequences and whole dna relatedness concur for the reassignment of pasteurella piscicida (snieszko et al.) janssen and surgalla to the genus photobacterium as photobacterium damsela subsp. piscicida comb. nov. | the taxonomic status of pasteurella piscicida (strain ncimb 2058t [t = type strain] and a strain isolated from the environment) was investigated by performing phylogenetic analyses of small-subunit rrna sequences, dna-dna hybridization analyses, and biochemical characterization analyses. the results of the phylogenetic analyses and the levels of dna-dna complementarity demonstrated conclusively that pasteurella piscicida is extremely closely related to photobacterium damsela atcc 33539t. since t ... | 1995 | 7531996 |
| purification and characterization of a marine bacterial beta-galactoside alpha 2,6-sialyltransferase from photobacterium damsela jt0160. | a bacterial sialyltransferase, named sialyltransferase 0160, was purified from a marine bacterium that had been isolated from seawater from sagami bay, kanagawa. this strain has been identified as photobacterium damsela, and named p. damsela jt0160. sialyltransferase 0160 was purified 688-fold to homogeneity from the crude extract of the cells with a yield of 19% using a combination of anion exchange chromatography, hydroxyapatite chromatography, gel filtration chromatography, and affinity chrom ... | 1996 | 8864851 |
| cloning and expression of a marine bacterial beta-galactoside alpha2,6-sialyltransferase gene from photobacterium damsela jt0160. | sialyltransferase 0160, a bacterial sialyltransferase which catalyzes the incorporation of neuac from cmp-neuac into the galactose residue of the carbohydrate chain at position 6, is produced by photobacterium damsela jt0160. the gene coding for sialyltransferase 0160 (bst) was cloned, sequenced, and expressed in escherichia coli. the sialyltransferase 0160 gene contains an open reading frame of 2,028 base pairs encoding a protein of 675 amino acid residues. the deduced amino acid sequence of si ... | 1998 | 9504414 |
| mass production of bacterial alpha 2,6-sialyltransferase and enzymatic syntheses of sialyloligosaccharides. | to supply alpha 2,6-sialyltransferase for the large-scale synthesis of sialoside, we investigated culture conditions for the production of sialyltransferase 0160. the addition of galactose and beef extract, and control of the ph of the culture medium were effective on the production of sialyltransferase 0160. the maximal enzyme productivity reached 550 units/l. using a crude extract of photobacterium damsela jt0160 cells as an enzyme source, enzymatic syntheses were performed with mono- and di-s ... | 1998 | 9532777 |
| phenotypic characterization of the marine pathogen photobacterium damselae subsp. piscicida. | the taxonomic position of photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, is controversial as this organism has also been described as 'pasteurella piscicida'. to clarify the taxonomic position of the pathogen, a total of 113 p. damselae subsp. piscicida strains and 20 p. damselae subsp. damselae strains, isolated from different geographical areas and from the main affected fish species, were analysed using 129 morphological and biochemical tests, including ... | 1998 | 9828416 |
| survival of fish-virulent strains of photobacterium damselae subsp. damselae in seawater under starvation conditions. | the survival of fish-virulent strains of photobacterium damselae subsp. damselae in seawater microcosms, with and without sediment, was investigated. the strains survived as culturable bacteria at 14 and 22 degrees c for at least 1 year, and infectivity for fish was maintained. at 5 degrees c, cells lost culturability on solid media, but this was recovered when the temperature was increased to 22 degrees c. finally, morphological changes in the bacterium (rod to coccus), and production of vesicl ... | 1998 | 9835027 |
| superoxide dismutase and catalase in photobacterium damselae subsp. piscicida and their roles in resistance to reactive oxygen species. | photobacterium damselae subsp. piscicida (formerly pasteurella piscicida) is the causative agent of pasteurellosis or pseudotuberculosis in warm water marine fish. enzymes which neutralize reactive oxygen species, produced during aerobic metabolism or during respiratory burst in fish macrophages, are important virulence factors in many pathogens. this study characterizes a periplasmic superoxide dismutase (sod) and a cytoplasmic catalase in p. damselae. purification and partial amino-terminal se ... | 1999 | 10075430 |
| vibrios associated with litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. | several bacteriological surveys were performed from 1994 to 1996 at different litopenaeus vannamei hatcheries (in ecuador) and shrimp farms (in mexico). samples were taken from routine productions of healthy and diseased l. vannamei larvae, postlarvae, and their culture environment and from healthy and diseased juveniles and broodstock. in ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been de ... | 1999 | 10347048 |
| 16s rrna gene sequence analysis of photobacterium damselae and nested pcr method for rapid detection of the causative agent of fish pasteurellosis. | the causative agent of fish pasteurellosis, the organism formerly known as pasteurella piscicida, has been reclassified as photobacterium damselae subsp. piscicida on the basis of 16s rrna gene sequence comparisons and chromosomal dna-dna hybridization data; thus, this organism belongs to the same species as photobacterium damselae subsp. damselae (formerly vibrio damselae). since reassignment of p. damselae subsp. piscicida was based on only two strains, one objective of the present work was to ... | 1999 | 10388687 |
| assessment of a magnetic bead-eia based kit for rapid diagnosis of fish pasteurellosis. | the accuracy of the magnetic beads-eia based bionor aquaeia-pp kit for the rapid diagnosis of pasteurellosis was evaluated. the kit reacted with all the photobacterium damselae subsp. piscicida strains included in this study, with a detection limit of 10(4) bacteria/ml. however, non-specific reactions were observed with isolates of ph. damselae subsp. damselae or ph. histaminum when the bacterial concentration was high (10(9)-10(10) bacteria/ml). similar findings in specificity and sensitivity w ... | 1999 | 10520595 |
| presence of phospholipase-d (dly) gene coding for damselysin production is not a pre-requisite for pathogenicity in photobacterium damselae subsp. damselae. | the presence of the phospholipase-d (dly) gene as pre-requisite for virulence of photobacterium damselae subsp. damselae for poikilotherm and homoiotherm animals was investigated in a total of 17 strains isolated from fish, shellfish, mammals and seawater. with this aim, we developed two pcr protocols. a simple pcr using primers flanking the almost complete dly gene, and a multiplex-pcr using two sets of primers directed to internal fragments of the dly and 16s rrna genes. only six of the 17 ph. ... | 2000 | 10644497 |
| invasion of fish epithelial cells by photobacterium damselae subsp. piscicida: evidence for receptor specificity, and effect of capsule and serum. | photobacterium damselae subsp. piscicida is a fish pathogen which causes serious disease in commercial warmwater fish species. because information on the initial stages of the infection is scarce, an investigation of the invasion ability of this pathogen was undertaken utilizing a fish epithelial cell line (epithelioma papillosum carpio, epc), a virulent capsulated strain of p. damselae (mt1415), an avirulent non-capsulated strain of p. damselae (epoy-8803-ii) and escherichia coli hb101 as a non ... | 2000 | 10658648 |
| necrotizing fasciitis due to photobacterium damsela in a man lashed by a stingray. | | 2000 | 10722346 |
| evidence that water transmits the disease caused by the fish pathogen photobacterium damselae subsp. damselae. | the transmission through water of the disease caused by the fish pathogen, photobacterium damselae subsp. damselae, as well as the role of the skin mucus in the initial steps of the infection, have been studied. all tested strains resisted the bactericidal activity of the mucus and showed an ability to adhere to it, but only those virulent by the intraperitoneal route were infective through water. moribund fishes showed the typical signs of the disease: haemorrhaged areas on the body surface and ... | 2000 | 10747234 |
| application of aflp for taxonomic and epidemiological studies of photobacterium damselae subsp. piscicida. | a collection of 106 photobacterium damselae subsp. piscicida strains and 19 photobacterium damselae subsp. damselae strains, including reference and type strains, were genetically characterized using aflp. the total genomic dna of each bacterial strain was digested using restriction endonucleases hindiii and taqi. using numerical analysis, six clusters were recognized. the largest cluster (n = 106) contained the majority of the strains tested and consisted exclusively of photobacterium damselae ... | 2000 | 10843040 |
| photobacterium histaminum okuzumi et al. 1994 is a later subjective synonym of photobacterium damselae subsp. damselae (love et al. 1981) smith et al. 1991. | the type strain of photobacterium histaminum, jcm 8968t (= atcc 51805t), and that of photobacterium damselae subsp. damselae, atcc 33539t, exhibit 100% identity in their 16s rrna sequence, more than 80% dna-dna homology and only one phenotypic difference. also, like p. histaminum, p. damselae subsp. damselae was shown to excrete a large amount of histamine when cells were grown on medium containing excessive histidine under acidic conditions. therefore, the name p. histaminum should be considere ... | 2000 | 10843080 |
| multiplex pcr assay for urec and 16s rrna genes clearly discriminates between both subspecies of photobacterium damselae. | a multiplex-pcr approach, employing 2 primer pairs directed to internal regions of the 16s rrna and urec genes, was utilized to analyze a collection of photobacterium damselae strains, including 25 isolates of subspecies piscicida and 15 isolates of subspecies damselae. with this procedure, all the p. damselae subsp. damselae strains yielded 2 amplification products, one of 267 bp and the other of 448 bp, corresponding to internal fragments of the 16s rrna and urec genes, respectively. however, ... | 2000 | 10843555 |
| molecular fingerprinting of strains of yersinia ruckeri serovar o1 and photobacterium damsela subsp. piscicida isolated in italy. | we studied the ribotypes, patterns of pulsed-field gel electrophoresis (pfge) and interspersed repeated sequences (irs)-pcr of 30 strains of yersinia ruckeri o1 and 20 strains of photobacterium damsela subsp. piscicida isolated from apparently unrelated epizootic outbreaks occurring on italian fish farms between 1993 and 1999. all of the y. ruckeri o1 strains had similar profiles, as demonstrated by all three typing methods, thus confirming the clonal structure of this species. the strains of p. ... | 2000 | 10973701 |
| existence of two geographically-linked clonal lineages in the bacterial fish pathogen photobacterium damselae subsp. piscicida evidenced by random amplified polymorphic dna analysis. | in this work, we applied the random amplified polymorphic dna (rapd) technique to evaluate the genetic diversity in photobacterium damselae subsp. piscicida (formerly pasteurella piscicida), an important pathogen for different marine fish. regardless of the oligonucleotide primer employed, the 29 isolates of ph. damselae subsp. piscicida tested were separated into two groups, the rapd-pcr analysis differentiated the european strains from the japanese strains. the similarity between both groups e ... | 2000 | 11057980 |
| large-scale production of the carbohydrate portion of the sialyl-tn epitope, alpha-neup5ac-(2-->6)-d-galpnac, through bacterial coupling. | alpha-neup5ac-(2-->6)-d-galpnac, the carbohydrate portion of sialyl-tn epitope of the tumor-associated carbohydrate antigen, was prepared by a whole-cell reaction through the combination of recombinant escherichia coli strains and corynebacterium ammoniagenes. two recombinant e. coli strains overexpressed the cmp-neup5ac biosynthetic genes and the alpha-(2-->6)-sialyltransferase gene of photobacterium damsela. c. ammoniagenes contributed to the production of utp from orotic acid. alpha-neup5ac-( ... | 2001 | 11269395 |
| the gill is a major organ for antibody secreting cell production following direct immersion of sea bass (dicentrarchus labrax, l.) in a photobacterium damselae ssp. piscicida bacterin: an ontogenetic study. | extremely high numbers of antibody secreting cells (asc) were observed in the gills of sea bass fry immunised at three different age/sizes (initial weight of 0.1, 2 and 5 g) by direct immersion in a photobacterium damselae spp. piscicida bacterin. the relatively low asc production in the head kidney and spleen suggests that the systemic compartment was only slightly stimulated upon immersion vaccination. there was no response of corresponding magnitude in the gut as the one observed in the gills ... | 2001 | 11271603 |
| electron microscopic evidence that expression of capsular polysaccharide by photobacterium damselae subsp. piscicida is dependent on iron availability and growth phase. | the expression of capsular polysaccharide (cps) by the fish pathogen photobacterium damselae subsp. piscicida was analysed in the virulent strain di 21 in relation to the growth phase and presence or absence of available iron in the culture medium. bacterial cells were processed for electron microscopy by a procedure that improves visualisation of the capsule through stabilisation with polycationic ferritin, and electron micrographs of ultrathin sections were scanned with an acquired computerise ... | 2001 | 11383572 |
| kinetics of juvenile sea bass (dicentrarchus labrax, l.) systemic and mucosal antibody secreting cell response to different antigens (photobacterium damselae spp. piscicida, vibrio anguillarum and dnp). | the elispot assay was used to measure the number of specific antibody secreting cells (asc) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (dicentrarchus labrax) to bacterial (vibrio anguillarum and photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (dnp-klh) antigens administered intraperitoneally. high variability among individuals was observed at each sampling day. a ... | 2001 | 11417719 |
| binding of haemin by the fish pathogen photobacterium damselae subsp. piscicida. | whole cells of virulent (di 21 and b 51) and avirulent (atcc 29690 and epoy 8803-ii) strains of photobacterium damselae subsp. piscicida, grown under iron-supplemented or iron-restricted conditions, were able to bind haemin. iron limitation resulted in an increased binding of haemin by di 21, b 51 and atcc 29690 cells but did not affect the haemin-binding ability of the epoy 8803-ii cells. proteinase k treatment of whole cells markedly reduced the binding of haemin, indicating that protein recep ... | 2002 | 12005232 |
| photobacterium damselae ssp. piscicida: detection by direct amplification of 16s rrna gene sequences and genotypic variation as determined by amplified fragment length polymorphism (aflp). | a pcr protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16s rrna gene sequences was developed. the procedure combines low annealing temperature that detects low titers of photobacterium damselae but also related species, and high annealing temperature for the specific identification of p. damselae directly from infected fish. the pcr protocol was validated on 19 piscine isolates of p. damselae ssp. piscicida from different geographic regions (japan, italy, spain, greece and isra ... | 2002 | 12033705 |
| photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen. | pasteurellosis, or pseudotuberculosis, is a bacterial septicaemia caused by the halophilic bacterium photobacterium damselae subsp. piscicida (formerly pasteurella piscicida). although this disease was first described in wild populations of white perch and striped bass, currently the natural hosts of the pathogen are a wide variety of marine fish. the disease has great economic impact both in japan, where it affects mainly yellowtail cultures, and in the mediterranean area, due to the losses it ... | 2002 | 12102234 |
| amplified fragment length polymorphism (aflp) and biochemical typing of photobacterium damselae subsp. damselae. | the aim of the present study was to characterize subspecifically photobacterium damselae subsp. damselae strains isolated from cultured sparus aurata and dicentrarchus labrax by means of phenotypic and molecular typing techniques (amplified fragment length polymorphism, aflp). | 2002 | 12234352 |
| the professional phagocytes of sea bass (dicentrarchus labrax l.): cytochemical characterisation of neutrophils and macrophages in the normal and inflamed peritoneal cavity. | in order to identify the phagocytic cells of sea bass, the peritoneal leucocyte population of fish injected intraperitoneally with photobacterium damselae subspecies piscicida was studied by light microscopy using cytocentrifuge preparations stained by the antonow technique for peroxidase detection. among the leucocytes present in the peritoneal exudate of the infected fish (macrophages, neutrophils, eosinophilic granular cells, lymphocytes and thrombocytes), macrophages and neutrophils were the ... | 2002 | 12365730 |
| diffusion of beta-lactam antibiotics through oligomeric or monomeric porin channels of some gram-negative bacteria. | the penetration of anionic beta-lactam antibiotics through porins was evaluated as a mechanism of drug resistance. the major proteins with porin activity were purified from the outer membranes of six bacteria. three of the six porins were oligomeric porins. the molecular weights of their monomers were 37 kda from photobacterium damsela, 42 kda from serratia liquefaciens, and 36 kda from e. coli b. the other three porins were heat-modifiable monomeric porins with molecular weights of 43 kda from ... | 2002 | 12402087 |
| numerical taxonomy of vibrionaceae isolated from cultured amberjack (seriola dumerili) and surrounding water. | a numerical taxonomic study was performed on 148 isolates of gram-negative, heterotrophic, facultative anaerobic bacteria isolated from amberjack (seriola dumerili) and its surrounding culture water. the study included 30 type and reference strains belonging to genera vibrio, listonella, and photobacterium. the strains were characterized by 109 morphological, biochemical, physiological, and nutritional tests. cluster analysis of similarity matrices obtained with s(sm) and s(j) coefficients was c ... | 2003 | 12567241 |
| cloning and nucleotide sequence analysis of the chloramphenicol resistance gene on conjugative r plasmids from the fish pathogen photobacterium damselae subsp. piscicida. | transferable resistance to various drugs was investigated in photobacterium damselae subsp. piscicida from japan. drug resistances were transferred via plasmids of 100, 50, and 40 kb. resistance to chloramphenicol (cmr) was transferred on plasmids of all 3 sizes. the cmr gene (cat) was cloned from the 50 kb plasmids ppdp8511 and ppdp9106 transferred from p. damselae subsp. piscicida strains isolated in different years and places in japan. subcloning localized the cat to within 1.5 kb hindiii-hin ... | 2003 | 12650243 |
| pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream sparus aurata. | the present study investigated the kinetic profile of flumequine (flu) in gilthead sea bream sparus aurata (170 g) held at 19 degrees c and evaluated its in vitro efficacy against important bacterial diseases in mediterranean mariculture. following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. tissue penetration of flu was low, since both t ... | 2003 | 12718468 |
| carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms. | a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ... | 2003 | 12747637 |
| apoptosis of sea bass (dicentrarchus labrax l.) neutrophils and macrophages induced by experimental infection with photobacterium damselae subsp. piscicida. | the infection of sea bass (dicentrarchus labrax l.) by intraperitoneal (i.p.) injection of the agent of fish pasteurellosis photobacterium damselae subsp. piscicida resulted in the apoptosis of peritoneal neutrophils and macrophages. all the eight virulent and none of the two non-virulent strains tested exhibited apoptogenic activity. a secreted bacterial protein(s) is a likely candidate as the factor(s) responsible for this activity, since no apoptosis was induced by i.p. injected uv-killed vir ... | 2003 | 12834617 |
| toxicity of nitric oxide and peroxynitrite to photobacterium damselae subsp. piscicida. | virulent strains of photobacterium damselae subsp. piscicida (pdp) were grown in media with or without glucose supplementation (to enhance polysaccharide capsule formation) and the bactericidal action of nitric oxide (no) and peroxynitrites was evaluated in a cell-free assay. treatment with the no-donor s-nitroso-acetyl-penicillamine (snap) induced a dose-and time-dependent decrease in pdp survival. this effect was greater for strains grown without glucose supplementation (c forms) than for thei ... | 2003 | 12892745 |
| investigation of media formulations promoting differential antigen expression by photobacterium damsela ssp. piscicida and recognition by sea bass, dicentrarchus labrax (l.), immune sera. | photobacterium damsela ssp. piscicida (phdp) isolates were grown in various bacteriological media, in eukaryotic cell culture media and in the presence of fish cells (resembling some aspects of in vivo growth environments). bacterial cells, extracellular products (ecps) and crude capsular polysaccharide were isolated and analysed by electrophoresis and western blot using sea bass sera. growth in bacteriological media conserved the synthesis of cell and extracellular components when these were co ... | 2003 | 12962207 |
| vaccination trials of sea bass, dicentrarchus labrax (l.), against photobacterium damsela subsp. piscicida, using novel vaccine mixtures. | bacterial cells of the marine fish pathogen photobacterium damsela subsp. piscicida were grown in novel culture media. a mixture of whole cells and extracellular components was inactivated and used in bath, intraperitoneal (i.p.) and oral vaccination of sea bass, dicentrarchus labrax, employing two sizes of fish. a commercial vaccine was used for comparative purposes. control and immunized fish were either bath or intraperitoneally challenged 6 and 12 weeks post-vaccination. small fish had signi ... | 2003 | 12962216 |
| survival against exogenous hydrogen peroxide of photobacterium damselae subsp. piscicida under different culture conditions. | | 2003 | 12962239 |
| purification and characterization of an antibacterial protein in the skin secretion of rockfish sebastes schlegeli. | an antibacterial protein in the skin secretion of rockfish (sebastes schlegeli) was purified by lectin affinity chromatography on con a-sepharose and gel filtration on tskgel g3000sw. the antibacterial protein featured the high molecular mass and selective action against gram-negative bacteria. the molecular mass of the protein was estimated to be approximately 150 kda in gel filtration and approximately 75 kda by sds-page, suggesting that it is dimeric. the antibacterial principle was an acidic ... | 2003 | 14522599 |
| virulence of photobacterium damselae subsp. piscicida in cultured cobia rachycentron canadum. | an outbreak of serious mortality among the cultured cobia rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in july of 2000 in taiwan. a non-motile strain cp1 was isolated from kidney and/or liver on tryptic soy agar and/or brain heart infusion agar plates (both supplemented with 1% nacl, w/v). this strain was characterized and identified as photobacterium damselae subsp. piscicida using biochemical chara ... | 2003 | 14625900 |
| simple and rapid detection of photobacterium damselae ssp. piscicida by a pcr technique and plating method. | to detect photobacterium damselae ssp. piscicida using the pcr technique and plating method. | 2003 | 14633013 |
| immune response of gilt-head seabream (sparus aurata) to antigens from photobacterium damselae subsp. piscicida. | | 2004 | 14675834 |
| construction of a safe, stable, efficacious vaccine against photobacterium damselae ssp. piscicida. | vaccination with bacterial auxotrophs, particularly those with an interruption in the common pathway of aromatic amino-acid biosynthesis, known as the shikimate pathway, has been shown to be effective in the prevention of a variety of bacterial diseases. in order to evaluate this approach to vaccine development in the important marine pathogen photobacterium damselae subsp. piscicida, the aroa gene of the shikimate pathway was identified from a p. damselae subsp. piscicida genomic library by com ... | 2003 | 14735921 |
| the effect of in vivo growth on the cellular and extracellular components of the marine bacterial pathogen photobacterium damsela subsp. piscicida. | photobacterium damsela subsp. piscicida, the causative agent of fish pasteurellosis, was grown in vivo. bacterial cells and extracellular products (ecps) were analysed via electrophoresis and immunoblot analysis, using specific sea bass antisera. growth in vivo induced the synthesis of unique bacterial cell proteins at > 206, 206, 21.3, 18, 7.6 and < 7.6 kda. sea bass serum raised against live bacterial cells of the pathogen and especially a sea bass serum raised against formalin-inactivated bac ... | 2004 | 14986934 |
| two cases of fatal necrotizing fasciitis caused by photobacterium damsela in japan. | we encountered two cases of fatal necrotizing fasciitis caused by photobacterium damsela in japan. both cases occurred in fishermen who became sick after fishing. they developed multiple organ failure within 20 to 36 h from the onset of initial symptoms despite intensive chemotherapy and surgical treatments. | 2004 | 15004123 |
| cloning and characterization of the gene encoding for omp-pd porin: the major photobacterium damsela outer membrane protein. | the outer membrane protein of photobacterium damsela (omp-pd) and the gene encoding for this porin protein were isolated and characterized. the deduced amino acid sequence of the omp-pd monomer has 338 amino acids and a calculated molecular weight of 36,951 da. this sequence includes a 22-amino acid signal peptide at the n-terminal, which is not found when the monomer is located in the outer membrane. native omp-pd protein forms a trimeric structure of approximately 110 kda. it exhibits resistan ... | 2004 | 15057460 |
| simultaneous detection of marine fish pathogens by using multiplex pcr and a dna microarray. | we coupled multiplex pcr and a dna microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (vibrio vulnificus, listonella anguillarum, photobacterium damselae subsp. damselae, aeromonas salmonicida subsp. salmonicida, and vibrio parahaemolyticus). the array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpon, gyrb, toxr, urec, dly, and vapa) and two plasmid-borne loci (fata and a.sa ... | 2004 | 15070982 |
| identification and characterisation of the fur genes in photobacterium damselae ssp. piscicida and ssp. damselae. | the gene encoding the ferric uptake regulator protein (fur gene) of the fish pathogenic bacterium photobacterium damselae ssp. piscicida strain d121 was partially amplified using degenerate oligonucleotides. complete sequencing of the fur gene and neighbouring dna was accomplished by primer walking. an open reading frame of 447 bp, coding for a protein of 148 amino acids, and with high homology to previously described fur proteins, was identified. the fur gene of p. damselae ssp. damselae atcc 3 ... | 2004 | 15109136 |
| activation of the nitric oxide response in gilthead seabream after experimental infection with photobacterium damselae subsp. piscicida. | inoculation of small gilthead seabream (sparus aurata) (30-75 g body weight) with a sublethal dose of different photobacterium damselae subsp. piscicida (pdp) strains (di-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (no) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. in contrast, no such response was detected in larger fish (150-600 g). since the virulence of pdp correlates with the presence of a polysaccharide capsular layer whi ... | 2004 | 15110332 |
| the effect of growth medium salinity of photobacterium damselae subsp. piscicida on the immune response of hybrid bass (morone saxatilis x m. chrysops). | photobacterium damselae subsp. piscicida (p. damselae) was grown on various media and the effect of media salinity on certain immune responses of hybrid bass was studied. in israel, pasteurellosis outbreaks have not been reported at water salinities below 1.38 per thousand. during vaccination experiments the salinity of the medium on which p. damselae is grown, was shown to affect stimulation of the immune system. no correlation was found between antibody response and protection. bacterial envel ... | 2004 | 15123315 |
| fatal infection of the hand due to photobacterium damsela: a case report. | we report a case of fatal acute renal failure due to photobacterium damsela infection of the hand. although the patient received intensive medical care and surgical treatment, he died 8 h after arrival at the hospital. this is the first case of p. damsela sepsis proven by blood culture in japan. | 2004 | 15156501 |
| retraction. fatal infection of the hand due to photobacterium damsela: a case report. | | 2004 | 15224696 |
| occurrence of tetracycline resistance genes tet(m) and tet(s) in bacteria from marine aquaculture sites. | occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in japan and korea. the tet(m) gene was detected in 34 japanese and korean isolates, which included vibrio sp., lactococcus garvieae, photobacterium damsela subsp. piscicida, and unidentified gram-positive bacteria. the majority of these bacterial isolates displayed high-level resistance with a minimum ... | 2004 | 15268950 |
| maternal transfer of humoral specific and non-specific immune parameters to sea bream (sparus aurata) larvae. | immunisation of sea bream (sparus aurata l.) broodstock with a novel vaccine mixture of photobacterium damsela subsp. piscicida sk7 (phdp) was performed during the period of egg development and the changes in specific and non-specific humoral immune parameters were measured. total immunoglobulin level, specific antibody titre, anti-protease activity and lysozyme activity were significantly higher in immunised parents compared to the control. after spawning significantly higher anti-protease acti ... | 2004 | 15313509 |
| influence of vaccination on the nitric oxide response of gilthead seabream following infection with photobacterium damselae subsp. piscicida. | the nitric oxide (no) response of vaccinated and non-vaccinated juvenile gilthead seabream was studied in vivo and the no response of isolated kidney macrophages of fish was studied in vitro. fish were vaccinated with formalin-killed photobacterium damselae subsp. piscicida (pdp) with or without freund's incomplete adjuvant (fia) and control fish received phosphate buffered saline (pbs). thirty days later, fish were injected with a sublethal dose of pdp and 3 fish/group were bled at time periods ... | 2005 | 15450966 |
| effect of dietary iron overload on photobacterium damselae ssp. piscicida pathogenicity in sea bass, dicentrarchus labrax (l.). | | 2004 | 15509262 |
| characterization of the 23s and 5s rrna genes and 23s-5s intergenic spacer region (its-2) of photobacterium damselae. | the 23s ribosomal rna (rrna) gene has been sequenced in strains of the fish pathogens photobacterium damselae subsp. damselae (atcc 33539) and subsp. piscicida (atcc 29690), showing that 3 nucleotide positions are clearly different between subspecies. in addition, the 5s rrna gene plus the intergenic spacer region between the 23s and 5s rrna genes (its-2) were amplified, cloned and sequenced for the 2 reference strains as well as the field isolates rg91 (subsp. damselae) and di21 (subsp. piscici ... | 2004 | 15584408 |
| effectiveness of a divalent vaccine for sole, solea senegalensis (kaup), against vibrio harveyi and photobacterium damselae subsp. piscicida. | the protection of cultured sole, solea senegalensis, against vibrio harveyi and photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective mo ... | 2005 | 15660791 |
| identification of fur regulated genes in the bacterial fish pathogen photobacterium damselae ssp. piscicida using the fur titration assay. | bacteria have developed a series of iron-scavenging and transport systems. the expression of many of the iron utilization genes is tightly regulated by the fe2+ loaded fur repressor protein. in this study, the fur titration assay (furta) was used to screen for dna fragments from a genomic dna library of photobacterium damselae ssp. piscicida containing potential fe2+ fur binding sites or iron binding-proteins which withdraw iron from fur. one of the clones encoded a tonb gene and adjacent a func ... | 2004 | 15689115 |
| variation in 16s-23s rrna intergenic spacer regions in photobacterium damselae: a mosaic-like structure. | phenotypically, photobacterium damselae subsp. piscicida and p. damselae subsp. damselae are easily distinguished. however, their 16s rrna gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of dna-dna similarity. the 16s-23s rrna internal transcribed spacers (its) were sequenced in two strains of photobacterium damselae subsp. piscicida and two strains of p. damselae subsp. damselae to determine the level of molecul ... | 2005 | 15691912 |
| molecular cloning and functional analysis of photobacterium damselae subsp. piscicida haem receptor gene. | a haem receptor gene from photobacterium damselae subsp. piscicida (formerly known as pasteurella piscicida) has been cloned, sequenced and analysed for its function. the gene, designated as pph, has an open reading frame consisting of 2154 bp, a predicted 718 amino acid residues and exists as a single copy. it is homologous with the haem receptors of vibrio anguillarum hupa, v. cholerae huta, v. mimicus mhua and v. vulnificus hupa at 32.7, 32.7, 45.6 and 30.9%, respectively, and is highly conse ... | 2005 | 15705153 |
| side effects in sea bass (dicentrarchus labrax l.) due to intraperitoneal vaccination against vibriosis and pasteurellosis. | sea bass (dicentrarchus labrax l.) were injected intraperitoneally with monovalent (photobacterium damselae subsp. piscicida or vibrio anguillarum) and divalent (ph. damselae subsp. piscicida and v. anguillarum) vaccine formulations, with or without adjuvants (mineral oil, liposome or alginate), to evaluate the short and long-term pathological effects. eight animals from each group were sampled one, two, four and 11 months after intraperitoneal injection. the acute peritoneal response and the pr ... | 2005 | 15722227 |
| antimicrobial substances from rhizomes of the giant knotweed polygonum sachalinense against the fish pathogen photobacterium damselae subsp. piscicida. | the antimicrobial compounds against the fish pathogen photobacterium damselae subsp. piscicida were isolated from polygonum sachalinense rhizomes. the structures of the antimicrobial compounds 1 and 2 were determined by 1h and 13c nmr, 2d-nmr (cosy, hsqc, hmbc and roesy) and fab-ms to be phenylpropanoid glycosides, vanicoside a and b, respectively. both compounds have feruloyl and p-coumaroyl groups bonded to a sucrose moiety in their structures. vanicoside a also has an acetyl group in the sucr ... | 2005 | 15787242 |
| adhesion to sole, solea senegalensis kaup, mucus of microorganisms isolated from farmed fish, and their interaction with photobacterium damselae subsp. piscicida. | abstract most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. however, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. nineteen isolates obtained from farmed senegalese sole, solea senegalensis kaup, and gilthead sea bream, sparus aurata l., have been evaluated for their cap ... | 2005 | 15813865 |
| the effect of sea bream (sparus aurata) broodstock and larval vaccination on the susceptibility by photobacterium damsela subsp. piscicida and on the humoral immune parameters. | sea bream broodstock were immunised 1 or 2 months before spawning with a novel photobacteriosis vaccine. sixty-seven-day-old larvae (mean weight 22.3 mg) originating from immunised and non-immunised parents were experimentally infected with the photobacterium damsela subsp. piscicida (phdp). larvae from immunised fish showed delayed onset and lower mortality (66.67%) compared with larvae from control fish (80%). eighty-nine-day-old larvae (mean weight 162.2 mg) from both groups were bath vaccina ... | 2005 | 15863015 |
| heme uptake genes in human and fish isolates of photobacterium damselae: existence of huta pseudogenes. | the marine bacterium photobacterium damselae includes strains classified into two distinct subspecies, namely subsp. damselae and subsp. piscicida, which have been reported to cause disease in a variety of marine animals and in humans. p. damselae strains utilize heme compounds as sole iron sources. in the present study, ten potential heme uptake and utilization genes are described in p. damselae subsp. damselae and subsp. piscicida. one gene cluster includes the genes coding for putative protei ... | 2005 | 15918073 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| update on bacterial vaccines: photobacterium damselae subsp. piscicida. | photobacterium damselae subsp. piscicida is the causative agent of pasteurellosis in wild and farmed marine fish worldwide. although serologically homogeneous, recent molecular advances have led to the discovery of distinct genetic clades, depending on geographical origin. further details of the strategies for host colonisation have arisen including information on the role of capsule, susceptibility to oxidative stress, confirmation of intracellular survival in host epithelial cells, and induced ... | 2005 | 15962471 |
| photobacterium damsela bacteremia in a child with sickle-cell disease. | photobacterium damsela was identified in a blood culture taken from a child with sickle-cell disease. this is the first report of this organism in humans in the caribbean. the microbiology of this organism and its identification are discussed. the clinical presentation in humans and the role of the immune status of the patient are reviewed. | 2005 | 15999016 |
| development of a simple and rapid fluorogenic procedure for identification of vibrionaceae family members. | we describe a simple colony overlay procedure for peptidases (copp) for the rapid fluorogenic detection and quantification of vibrionaceae from seawater, shellfish, sewage, and clinical samples. the assay detects phosphoglucose isomerase with a lysyl aminopeptidase activity that is produced by vibrionaceae family members. overnight cultures are overlaid for 10 min with membranes containing a synthetic substrate, and the membranes are examined for fluorescent foci under uv illumination. fluoresce ... | 2005 | 16000757 |
| quorum sensing signal molecules (acylated homoserine lactones) in gram-negative fish pathogenic bacteria. | the aim of the present study was to investigate the production of quorum sensing signals (specifically acylated homoserine lactones, ahls) among a selection of strains of gram-negative fish bacterial pathogens. these signals are involved in the regulation of virulence factors in some human and plant-pathogenic bacteria. a total of 59 strains, representing 9 different fish pathogenic species, were tested against 2 ahl monitor bacteria (agrobacterium tumefaciens nt1 [pzlr4] and chromobacterium vio ... | 2005 | 16042042 |
| direct identification of photobacterium damselae subspecies piscicida by pcr-rflp analysis. | fish pasteurellosis is an infectious disease that affects several teleost species living in temperate marine waters. the pathogen responsible, photobacterium damselae subspecies piscicida, shows high genetic similarity with p. damselae subsp. damselae, making subspecies discrimination extremely laborious. here we report for the first time a pcr-rflp method for the identification of p. damselae subsp. piscicida without prior isolation in pure culture. genomic sequence information was obtained thr ... | 2005 | 16042043 |
| subtractive hybridization reveals a high genetic diversity in the fish pathogen photobacterium damselae subsp. piscicida: evidence of a sxt-like element. | photobacterium damselae subsp. piscicida is the causative agent of fish pasteurellosis, a severe disease affecting cultured marine fish worldwide. in this study, suppression subtractive hybridization was used to identify dna fragments present in the virulent strain pc554.2, but absent in the avirulent strain epoy 8803-ii. twenty-one genomic regions of this type (that included twenty-six distinct putative orfs) were analysed by dna sequencing. twenty orfs encoded proteins with homology to protein ... | 2005 | 16079344 |
| detection of quinolone-resistance genes in photobacterium damselae subsp. piscicida strains by targeting-induced local lesions in genomes. | quinolone-resistant strains of the fish-pathogenic bacterium, photobacterium damselae subsp. piscicida are distributed widely in cultured yellowtail, seriola quinqueradiata (temminck & schlegel), in japan. the quinolone resistance-determining region (qrdr) was amplified with degenerate primers, followed by cassette ligation-mediated pcr. open reading frames encoding proteins of 875 and 755 amino acid residues were detected in the gyra and parc genes, respectively. resistant strains of p. damsela ... | 2005 | 16159364 |
| effect of salinity on the immune response of tiger shrimp penaeus monodon and its susceptibility to photobacterium damselae subsp. damselae. | addition of nacl at 2.5% to tryptic soy broth (tsb) significantly increased the growth of photobacterium damselae subsp. damselae. tiger shrimp penaeus monodon held in 25 per thousand seawater were injected with p. damsela subsp. damselae grown in tsb containing nacl at 0.5%, 1.5%, 2.5% and 3.5% at a dose of 8.48 x 10(4)colony-forming units (cfu)shrimp(-1). over 24-96 h, the cumulative mortality was significantly higher for the shrimp challenged with p. damselae subsp. damselae grown in 2.5% nac ... | 2006 | 16213751 |
| aip56, a novel plasmid-encoded virulence factor of photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils. | a strategy used by extracellular pathogens to evade phagocytosis is the utilization of exotoxins that kill host phagocytes. we have recently shown that one major pathogenicity strategy of photobacterium damselae subsp. piscicida (phdp), the agent of the widespread fish pasteurellosis, is the induction of extensive apoptosis of sea bass macrophages and neutrophils that results in lysis of these phagocytes by post-apoptotic secondary necrosis. here we show that this unique process is mediated by a ... | 2005 | 16262788 |
| complement consumption by photobacterium damselae subsp. piscicida in seabream, red porgy and seabass normal and immune serum. effect of the capsule on the bactericidal effect. | a virulent strain of photobacterium damselae subsp. piscicida (pdp) was grown without (c form) or with (c+ form) glucose supplementation, the latter to enhance capsule formation. both forms were resistant to killing by normal serum of seabream, red porgy and seabass. however, the c form was killed by immune serum of all three fish species while the c+ form was killed only by seabream and red porgy sera and to a lesser extent than the c form. both c and c+ forms consumed complement in normal seru ... | 2006 | 16263309 |
| first isolation of photobacterium damselae ssp. damselae from cultured redbanded seabream, pagrus auriga valenciennes, in spain. | | 2006 | 16533303 |
| photobacterium ganghwense sp. nov., a halophilic bacterium isolated from sea water. | a marine bacterial strain, designated fr1311t, was isolated from a sea-water sample from ganghwa island, south korea. cells were gram-negative, facultatively anaerobic, catalase- and oxidase-positive, motile, oval or rod-shaped and halophilic (optimum sea-salt concentration for growth of 5-6 %). phylogenetic analysis of its 16s rrna gene sequence revealed that it represented a distinct line of descent within the genus photobacterium. the major fatty acids were straight-chain saturated (c(16 : 0) ... | 2006 | 16585687 |
| aldolase-catalyzed synthesis of beta-d-galp-(1-->9)-d-kdn: a novel acceptor for sialyltransferases. | [reaction: see text] beta-d-galp-(1-->9)-d-kdn, a disaccharide component of the cell wall of streptomyces sp. mb-8, was synthesized from beta-d-galp-(1-->6)-d-manp and pyruvate using a sialic acid aldolase. the obtained kdn-containing compound was a novel acceptor for bacterial sialyltransferases. unusual alpha2,3- and alpha2,6-linked sialyltrisaccharides and a tetrasaccharide were synthesized using a one-pot two-enzyme system containing a neisseria meningitidis cmp-sialic acid synthetase and a ... | 2006 | 16706534 |
| drug resistance and random amplified polymorphic dna analysis of photobacterium damselae ssp. piscicida isolates from cultured seriola (yellowtail, amberjack and kingfish) in japan. | to investigate the antimicrobial susceptibility and genetic characteristics of photobacterium damselae ssp. piscicida isolates obtained from cultured seriola in japan. | 2006 | 16706907 |
| infection with photobacterium damselae subspecies damselae and vibrio harveyi in snapper, pagrus auratus with bloat. | to diagnose the cause of chronic, low mortality associated with bloat in tanks of snapper at an aquaculture facility. | 2006 | 16739527 |
| superoxide dismutase and catalase activities in photobacterium damselae ssp. piscicida. | the ability of a set of photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (sod) and catalase enzymes was determined. unlike other bacterial pathogens, p. damselae ssp. piscicida is not able to produce different isoforms of sod or catalase containing different metal cofactors when cultured under oxidative stress induced by hydrogen peroxide or methyl viologen, or under iron depleted conditions. however, iron content of th ... | 2006 | 16768716 |
| molecular cloning and characterisation of sea bass (dicentrarchus labrax l.) caspase-3 gene. | caspase-3 is one of the major caspases operating in apoptosis, cleaving and inactivating a number of molecules and largely contributing to the apoptotic phenotype and the dismantling of the apoptoting cell. the opening reading frame of sea bass (dicentrarchus labrax l.) caspase-3 has 281 amino acids. the complete sequence of caspase-3 shows a very close homology to the correspondent sequence from other vertebrates, in particularly with that of takifugu rubripes and oryzias latipes, with 87.7 and ... | 2007 | 16780952 |
| ompw and ompv are required for nacl regulation in photobacterium damsela. | photobacterium damsela is a marine pathogen to both fish and human beings. the bacterium can shift between the ambient seawater and hosts, suggesting the existence of proteins rapidly responding to salt concentration. in the current study, proteomic methodologies were applied to screen the outer membrane proteins (omps) related to salt stress. ompw and ompv were determined in the response in this bacterium as ompc and ompf did in e. coli. furthermore, the two genes were overexpressed in e. coli ... | 2006 | 16944937 |
| first molecular cloning and characterisation of caspase-9 gene in fish and its involvement in a gram negative septicaemia. | caspase-9 is an initiator caspase in the apoptotic process whose function is to activate effector caspases that are downstream in the mitochondrial pathway of apoptosis. this work reports for the first time the complete sequencing and characterisation of caspase-9 in fish. a 1924bp cdna of sea bass caspase-9 was obtained, consisting of 1308bp open reading frame coding for 435 amino acids, 199bp of the 5'-utr and 417bp of the 3'-utr including a canonical polyadenilation signal 10 nucleotides upst ... | 2007 | 16989898 |
| characterization of japanese flounder (paralichthys olivaceus) nk-lysin, an antimicrobial peptide. | the nk-lysin cdna of japanese flounder, paralichthys olivaceus, consists of 657bp, containing an open reading frame (orf) of 444bp, which encodes 147 amino acid residues. the amino acid sequence of japanese flounder nk-lysin has 21% identity to porcine nk-lysin and bovine nk-lysin, 23% to equine nk-lysin, and 46% to zebrafish nk-lysin-like protein. multiple alignments of japanese flounder nk-lysin and other known saposin-like proteins revealed that the six cysteine residues important for structu ... | 2007 | 17046282 |
| molecular characterization, 3d modelling and expression analysis of sea bass (dicentrarchus labrax l.) interleukin-10. | interleukin-10 (il-10) is a pleiotropic cytokine generally known for its relevance in the resolution of inflammation, but that also has immunostimulatory properties. here is described the isolation and characterization of the sea bass il-10 (sbil-10) cdna and gene. the sbil-10 gene encodes a 187 amino acid protein and comprises a five exon-four intron structure as other known il-10 genes. important structural residues are maintained in the sbil-10 protein, including the four cysteines responsibl ... | 2007 | 17049605 |
| a siderophore biosynthesis gene cluster from the fish pathogen photobacterium damselae subsp. piscicida is structurally and functionally related to the yersinia high-pathogenicity island. | photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, produces a siderophore which is distinct from that produced by p. damselae subsp. damselae. using suppression subtractive hybridization, a subsp. piscicida-specific dna region of 35 kb was identified in strain di21, and 11 genes were defined: dahp, arac1, arac2, frpa, irp8, irp2, irp1, irp3, irp4, irp9 and irp5. the sequence of the predicted proteins encoded by these genes showed significant similarity with the ... | 2006 | 17074903 |
| identification of an antibacterial protein as l-amino acid oxidase in the skin mucus of rockfish sebastes schlegeli. | fish skin mucus contains a variety of antimicrobial proteins and peptides that seem to play a role in self defense. we previously reported an antibacterial protein in the skin secretion of the rockfish, sebastes schlegeli, which showed selective antibacterial activity against gram-negative bacteria. this study aimed to isolate and structurally and functionally characterize this protein. the antibacterial protein, termed ssap (s. schlegeli antibacterial protein), was purified to homogeneity by le ... | 2007 | 17140417 |
| cloning, promoter analysis and expression in response to bacterial exposure of sea bass (dicentrarchus labrax l.) interleukin-12 p40 and p35 subunits. | interleukin-12 (il-12) is a heterodimeric cytokine pivotal in resistance to microbial and viral infections. in the search for immunoregulatory genes in sea bass the genes for the two il-12 subunits p40 and p35 were cloned and sequenced. molecular characterization of these two genes was performed at both the cdna and genomic levels. sea bass il-12 p40 and p35 conserve most cysteines involved in the intra-chain disulfide bonds of human il-12 subunits as well as the important structural residues fo ... | 2007 | 17196658 |
| heme, an iron supply for vibrios pathogenic for fish. | one of the main mechanisms present in gram-negative bacterial pathogens to obtain iron is the utilization of free heme or heme proteins from the host tissues. vibrio anguillarum, the etiological agent of vibriosis in fish, and photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, can acquire iron from free heme or heme-containing proteins present in the host tissues by a siderophore-independent mechanism. similarly to other animal and human pathogens, the general ... | 2007 | 17206385 |
| dopamine depresses immunity in the tiger shrimp penaeus monodon. | the total haemocyte count (thc), differential haemocyte count (dhc), phenoloxidase activity, respiratory bursts (release of superoxide anion), superoxide dismutase activity, phagocytic activity and clearance efficiency to the pathogen photobacterium damsela were measured when tiger shrimp penaeus monodon (13.5+/-1.5 g) were individually injected with saline or dopamine at 10(-8), 10(-7), or 10(-6)mol shrimp(-1). results showed that a transient period of immunosuppression occurred between 2 and 8 ... | 2007 | 17210259 |
| histidine decarboxylases and their role in accumulation of histamine in tuna and dried saury. | histamine-producing bacteria (hpb) such as photobacterium phosphoreum and raoultella planticola possess histidine decarboxylase (hdc), which converts histidine into histamine. histamine fish poisoning (hfp) is attributable to the ingestion of fish containing high levels of histamine produced by hpb. because freezing greatly decreases the histamine-producing ability of hpb, especially of p. phosphoreum, it has been speculated that hfp is caused by hdc itself from hpb cells autolyzing during froze ... | 2007 | 17220267 |
| cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo. | detachment-induced apoptosis of enterocytes (anoikis) has not been investigated in vivo. here we describe anoikis of fish enterocytes following detachment in a septicemia by photobacterium damselae subsp. piscicida, or following injection of its exotoxin. the in vivo study was complemented with an ex vivo time-lapse analysis using conditions duplicating the in vivo situation. linings of enterocytes detached from intestine mucosa dissociate into isolated enterocytes which undergo caspase 3-mediat ... | 2007 | 17245641 |
| expression, characterization and immunogenicity of a major outer membrane protein from vibrio alginolyticus. | vibrio alginolyticus is one of the vibrio pathogens common to humans and marine animals. during infection and induction of the host immune response, outer membrane proteins of bacteria play an important role. in this study, an outer membrane protein gene (ompw) was cloned from v. alginolyticus and expressed in escherichia coli. the 645 bp open reading frame (orf) encodes a protein of 214 amino acid residues with a predicted molecular weight of 23.3 kda. the amino acid sequence showed a high iden ... | 2007 | 17342258 |
| systemic macrophage and neutrophil destruction by secondary necrosis induced by a bacterial exotoxin in a gram-negative septicaemia. | bacterial modulation of phagocyte cell death is an emerging theme in pathogenesis. here we describe the systemic destruction of macrophages and neutrophils by the gram-negative photobacterium damselae ssp. piscicida (phdp) in fish pasteurellosis, a deadly systemic infection. following experimental inoculation, phdp spreads by bacteraemia and colonizes the organs, producing a septicaemic infection, and secretes the apoptogenic exotoxin aip56 which is systemically disseminated. in experimental and ... | 2007 | 17381431 |
| molecular cloning and expression analysis of sea bass (dicentrarchus labrax l.) tumor necrosis factor-alpha (tnf-alpha). | in the search for pro-inflammatory genes in sea bass a tnf-alpha gene was cloned and sequenced. the sea bass tnf-alpha (sbtnf-alpha) putative protein conserves the tnf-alpha family signature, as well as the two cysteines usually involved in the formation of a disulfide bond. the mouse tnf-alpha thr-leu cleavage sequence and a potential transmembrane domain were also found, suggesting that sbtnf-alpha exists as two forms: a approximately 28 kda membrane-bound form and a approximately 18.4 kda sol ... | 2007 | 17433716 |
| an unusual case of urinary tract infection in a pregnant woman with photobacterium damsela. | we describe a case of a urinary tract infection with an unusual pathogen, photobacterium damsela, in a pregnant female. this pathogen has been described as having a virulent life threatening nature, so a detailed history and prompt treatment is needed. | 2006 | 17485811 |