| an endogenous oncornavirus of guinea pigs: its expression in leukemic cells. | guinea pigs do not have any known infectious oncornaviruses. we have recently activated an endogenous oncornavirus (gpv) from cultured guinea pig cells after bromodeoxyuridine riboside (budr) treatment. gpv is noninfectious for guinea pig cells and is inducible from any guinea pig cell in culture. although the morphogenesis of the activated virus is unique, it has the morphology and density (1.16 g/ml) of type c oncornaviruses. it contains an oncornavirus specific reverse transcriptase and a hig ... | 1975 | 51631 |
| biochemical properties of the bromodeoxyuridine-induced guinea pig virus. | the biophysical and biochemical properties of the virus particles released by guinea pig embryo cells treated with 5-bromo-2'-deoxyuridine (budr) have been compared to those of the b-type mouse mammary tumor virus (mmtv) and the c-type rauscher murine leukemia virus. the high-molecular-weight (60 to 70s) rna of the budr-induced guinea pig virus (gpv) has a molecular weight of 8 x 106 when measred by mixed agarose polyacylamide gel electrophoresis. the virus particles isolated from the tissue cul ... | 1975 | 51933 |
| expression of endogenous retroviral genes in leukemic guinea pig cells. | the expression of guinea pig retrovirus (5-bromodeoxyuridine[budr]-induced gpv) was studied in guinea pig l(2)c leukemic lymphoblasts by use of molecular hybridization of viral complementary dna (cdna) to cellular rna. it was found that l(2)c leukemic lymphoblasts, leukemic spleen, and budr-induced virus-producing cells contain virus-specific rna: 0.05% (800 to 960 copies per cell), 0.02% (360 copies per cell), and 0.3% (5,120 copies per cell), respectively. adult normal liver and spleen, on the ... | 1977 | 69723 |
| interaction in replication between goose parvovirus strain b and duck plague herpesvirus. | the goose parvovirus strain b was found to replicate only in cells with dna synthesis. cells in the stationary phase of dna synthesis or maintained under 2 mm thymidine (tdr) in excess were refractive to the parvovirus infection. however, stationary phase cells could be rendered to produce progeny paroviruses by herpesvirus superinfection. in reverse, the replication of the herpesvirus was significantly inhibited by parvovirus preinfection of cells. | 1979 | 426633 |
| effect of mycoplasma gallinarum on the replication in vitro of goose parvovirus strain "b". | replication in vitro of the goose parovirus strain "b" was inhibited by mycoplasma gallinarum co-infection. this effect could be prevented by continuous supplementation of cell cultures with arginine. the infection of cell cultures with acholeplasma axanthum did not influence virus replication. | 1975 | 1171580 |
| kinetics and viral protein specificity of the cytotoxic t lymphocyte response in healthy adults immunized with live attenuated varicella vaccine. | the cytotoxic t lymphocyte (ctl) response was evaluated in adults given live attenuated varicella vaccine, using target cells expressing varicella-zoster virus (vzv) immediate-early protein (ie62) or vzv glycoproteins gpi, gpiv, or gpv to determine viral protein specificity. the frequency of ctl that recognized ie62 was 1:171,000 +/- 46,000 se in subjects tested 10 days to 8 weeks after the initial vaccine dose; the induction of ctl specific for gpi was equivalent. ctl recognition of vzv protein ... | 1992 | 1314871 |
| receptor properties of two varicella-zoster virus glycoproteins, gpi and gpiv, homologous to herpes simplex virus ge and gi. | the varicella-zoster virus (vzv) genome contains 70 reading frames (orf), 5 of which encode the glycoproteins gpi, gpii, gpiii, gpiv, and gpv. orf 67 and 68 lie adjacent to each other in the unique short region of the vzv genome and code for gpiv and gpi, respectively. these two genes, which are contained within the hindiii c fragment of the vzv genome, were subcloned in the correct orientation downstream from the promoter regions of the eukaryotic expression vectors pcmv5 and pbj. after transfe ... | 1992 | 1316474 |
| transcription from varicella-zoster virus gene 67 (glycoprotein iv). | three transcripts map to the varicella-zoster virus (vzv) open reading frame (orf) 67, which encodes glycoprotein iv (gpiv). all of these transcripts are polyadenylated and are transcribed from left to right towards the genomic terminal short repeats. previous northern (rna) blot analyses suggested that the most abundant of these transcripts (1.65 kb) might code for gpiv. we performed s1 nuclease protection and primer extension assays and determined that the 5' terminus of the 1.65-kb transcript ... | 1992 | 1316476 |
| immunity in strain 2 guinea-pigs inoculated with vaccinia virus recombinants expressing varicella-zoster virus glycoproteins i, iv, v or the protein product of the immediate early gene 62. | the immunogenicity of specific varicella-zoster virus (vzv) proteins, with emphasis upon cell-mediated immune responses, was evaluated by immunizing strain 2 guinea-pigs with vaccinia virus recombinants that express gpi (vac-gpi), gpiv (vac-gpiv) and gpv (vac-gpv) or the ie-62 protein (vac-ie-62). vac-gpi elicited the highest initial mean t cell proliferation response [stimulation index (s.i.) 3.8 +/- 0.9 s.e.m.] whereas inoculation with vac-gpv produced the lowest primary t cell response (s.i. ... | 1992 | 1321876 |
| a detailed analysis of transcripts mapping to varicella zoster virus gene 14 (glycoprotein v). | we have characterized in detail those transcripts mapping to the varicella zoster virus (vzv) glycoprotein v (gpv) open reading frame (orf). the analyses revealed that a major 1.95-kb and a minor 2.5-kb transcript map to these sequences for vzv strain scott, with 1.8- and 2.3-kb transcripts in a vaccine oka strain, consistent with intragenic repeat (r2) copy number differences. the transcripts for each strain are 5' coterminal, are polyadenylated, and do not seem to be spliced. upstream of the c ... | 1991 | 1653492 |
| differentiation of oka varicella vaccine strain from wild varicella-zoster virus strains isolated from vaccinees and household contact. | the oka varicella vaccine strain can be differentiated from wild-type strains by its unique restriction endonuclease fingerprinting (refp: hpai-k and ecori-p) pattern of the gpv-coding region of the varicella-zoster virus (vzv) genome. vzv-dnas from patients with complicated clinical courses related to vaccination were examined to determine whether they were vaccine-derived or wild-type. a virus was isolated from a one year-old boy with acute lymphocytic leukemia (all) who developed typical vari ... | 1991 | 1675658 |
| molecular biology of the viral haemorrhagic disease virus of rabbits. | viral haemorrhagic disease virus (vhdv) of rabbits is a small, nonenveloped dna virus which contains a linear, single-stranded genome approximately 7,000 bases in length. the virions are icosahedral in symmetry, consisting of 180 subunits with pentamer-hexamer clustering. the capsid is composed of four structural polypeptides: vp1:60-61 kda, vp2:54.7 kda, vp3:52 kda, and vp4:26-28 kda. vp1 is a major structural polypeptide which constitutes approximately 54.7% of the total viral proteins. there ... | 1991 | 1760580 |
| a possible role for glycoprotein gpv in the pathogenesis of varicella-zoster virus. | | 1990 | 1963048 |
| mechanism of length determination in bacteriophage lambda tails. | the mechanism of length determination in bacteriophage lambda tails is discussed as a model for regulation in protein assembly systems. the lambda tail is a long flexible tube ending in a conical part and a single tail fiber. its length is exactly determined in the sense that the number of major tail protein (gpv) molecules, which comprise more than 80% of the mass of the tail, is exactly the same in all tails. assembly of gpv is regulated by the initiator complex, which contains the tail fiber ... | 1990 | 2150582 |
| the glycoprotein products of varicella-zoster virus gene 14 and their defective accumulation in a vaccine strain (oka). | many characteristics of the putative protein encoded by varicella-zoster virus (vzv) open reading fram (orf) 14 indicate that it is a glycoprotein, which has been designated gpv. to identify the protein products of the gene, the coding sequences were placed under the control of the vaccinia virus p7.5 promoter and recombinant vaccinia viruses were constructed. heterogeneous polypeptides with molecular weights of 95,000 to 105,000 (95k to 105k polypeptides) were expressed in cells infected by a v ... | 1990 | 2166829 |
| the nucleotide sequence of the equine herpesvirus 4 gc gene homologue. | the genomic position of an equine herpesvirus 4 (ehv-4) gene homologue of the herpes simplex virus 1 (hsv-1) gc gene was determined by southern analysis and dna sequencing. the gene lies within a 2-kbp bg/ii-ecori fragment mapping between 0.15 and 0.17 within the long unique component of the ehv-4 genome and is transcribed from right to left. putative promoter elements were identified upstream of the 1455-bp open reading frame which encodes a 485-amino-acid protein of unglycosylated molecular we ... | 1990 | 2171212 |
| glycoproteins encoded by varicella-zoster virus: biosynthesis, phosphorylation, and intracellular trafficking. | this article reviews major developments concerning the vzv glycoproteins. very little was published about these viral products prior to the early 1980s. vzv is now known to encode five glycoproteins that have been designated gpi through gpv. the viral glycoproteins are present within the envelope of the complete virion; in addition, they are inserted in the plasma membrane of the infected cell. studies of the assembly and processing of three of the vzv glycoproteins have demonstrated that they m ... | 1990 | 2174668 |
| antigenic relationships among autonomous parvoviruses. | the antigenic relatedness of minute virus of mice (mvm), kilham rat virus (kr), h-1 virus (h-1), haemorrhagic encephalopathy of rats virus (her), porcine parvovirus (ppv), canine parvovirus (cpv), feline panleukopenia virus (fpv), goose parvovirus (gpv) and bovine parvovirus (bpv) was studied by immunofluorescence microscopy (fa) and by serum neutralization (sn). an antigenically related group comprising mvm, kr, her, ppv, cpv and fpv was recognized by fa and most reactions within the group were ... | 1986 | 2432167 |
| expression in recombinant vaccinia virus of the equine herpesvirus 1 gene encoding glycoprotein gp13 and protection of immunized animals. | the equine herpesvirus 1 (ehv-1) gene encoding glycoprotein 13 (gp13) was cloned into the hemagglutinin (ha) locus of vaccinia virus (copenhagen strain). expression of the gp13 gene was driven by the early/late vaccinia virus h6 promoter. metabolically radiolabeled polypeptides of approximately 47 and 44 kilodaltons and 90 kilodaltons (glycosylated form) were precipitated with both polyclonal and gp13-specific monoclonal antibodies. presentation of gp13 on the cytoplasmic membrane of cells infec ... | 1989 | 2550665 |
| comparison of the sequence of the secretory glycoprotein a (ga) gene in md5 and bc-1 strains of marek's disease virus type 1. | dna fragments containing the secretory glycoprotein a (ga) gene of marek's disease virus type 1 (mdv1) were cloned from the dna libraries of very virulent md5 and virulent bc-1 strains and sequenced. two open reading frames (orf1 and orf2) were identified for both strains. the orf1 has the potential to code for a protein of 501 amino acids with a molecular weight of 56 kd that contains strong hydrophobic regions in both the amino and carboxyl termini, and nine potential n-linked glycosylation si ... | 1989 | 2559540 |
| congenital varicella-zoster virus infection and the failure to establish virus-specific cell-mediated immunity. | varicella-zoster virus (vzv) is one of the six human herpesviruses. the viral genome encodes five glycoproteins among its 70 open reading frames; these have been designated gpi to gpv. vzv causes the primary disease chickenpox, usually in children, after which the virus remains latent in the dorsal root ganglia. later in life, vzv reactivates and causes the disease zoster. vzv can also infect the fetus of a pregnant woman who contracts chickenpox. the fetopathy is unusual in that it more closely ... | 1989 | 2560525 |
| detection of goat pox antigen and antibody by the counter immunoelectrophoresis test. | the counterimmunoelectrophoresis (cie) test was standardised for the detection of goat pox antigen and antibody using inactivated antigens. the chloroform inactivated and live antigens were equally sensitive for detection of goat pox precipitins. the precipitinogens of goat pox virus (gpv) were found to be soluble in nature. the cie test was quick as well as more sensitive than the agar gel precipitation test for detection of gpv antibody/antigen. the cie employing inactivated antigen has been u ... | 1988 | 2840760 |
| application of elisa in the detection of goat pox antigen and antibodies. | enzyme linked immunosorbent assay (elisa) was standardized for detection of goat pox virus (gpv) antibodies and antigen using live and inactivated antigens and hyperimmune serum (his), convalescent, post-vaccinal, as also post-challenge sera. the elisa was most sensitive in detection of antibody when compared with agar gel precipitation (agp) and counterimmunoelectrophoresis (cie) tests. there was no complete correlation between the antibody status of vaccinated goats and protective immunity as ... | 1988 | 2897774 |
| [vaccination of geese (anser anser dom.) and muscovy ducks (cairina moschata dom.) against parvovirus hepatitis (s. derzsy's disease)-- report of a field trial with the attenuated live virus vaccine palmivax]. | | 1985 | 2996870 |
| effect of a preliminary infection of ducks with eds-76 virus on the derzsy's disease pathology. | the aim of this work was to investigate the role of a non pathogenic adenovirus (eds-76, strain a-127), frequently isolated from ducklings on the infection by a pathogen agent (such as the parvovirus responsible for derzsy's disease, ddv). three groups of ducklings were respectively infected with a-127 alone, at day old, ddv alone at day 10th, a-127 at day old plus ddv at day 10th. the infected groups and a control one were raised under similar conditions. pathological signs were observed and bo ... | 1987 | 3034130 |
| [pathology and electron microscopic demonstration of viruses in tissues in derzsy's disease (parvovirus infection) of goslings]. | | 1987 | 3038050 |
| characterization of bromodeoxyuridine-induced endogenous guinea pig virus. | an endogenous virus (gpv) was induced after 5-bromodeoxyuridine treatment of cultured guinea pig cells. compared to gross murine leukemia virus (g-mulv) gpv has a reproducibly heterogenous density of about 1.16 to 1.18 g/ml. the virion-associated rna is slightly larger than that in g-mulv. sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of dissociated gpv resolved five major structural proteins: i (molecular weight 70,000), ii (molecular weight 36,000), iii (molecular weight 2 ... | 1974 | 4368486 |
| some growth characteristics of goose parvovirus strain "b". | | 1974 | 4456997 |
| discriminated selection among viral peptides with the appropriate anchor residues: implications for the size of the cytotoxic t-lymphocyte repertoire and control of viral infection. | structural characterization of peptides restricted by major histocompatibility complex (mhc) class i molecules has identified residues critical for mhc class i binding and for t-cell receptor recognition. for example, optimal peptides fitting into the murine mhc class i db groove are 9 to 11 amino acids long and require as mhc anchor residues an asn (n) at position 5 and also either a hydrophobic residue, a met (m) or a cys (c), at the carboxy terminus. the three known db-restricted peptides of ... | 1995 | 7494247 |
| goose parvovirus--an autonomous member of the dependovirus genus? | goose parvovirus is the etiological agent of derzsy's disease, a fatal hepatitis of young geese. the virus infects geese and muscovy ducks and can be propagated in the laboratory in primary embryonic goose fibroblasts. to date the virus has only been classified by morphological, biochemical, and culture characteristics as an autonomous parvovirus. we now report the cloning and partial sequencing of 3434 nucleotides of the viral genome. three overlapping clones were obtained, encoding regions in ... | 1995 | 7618268 |
| ctl escape viral variants. ii. biologic activity in vivo. | the proteins of lymphocytic choriomeningitis virus (lcmv) contain only three known peptide regions that are processed and then held in place by the mhc class i h-2b (db) glycoprotein on the cell's surface for recognition by lcmv-specific db-restricted cytotoxic t lymphocytes (ctl). these peptides are from the glycoprotein (gp), amino acids 33-41 kavynfatc (gp1) and 276-286 sgvenpggycl (gp2), and the nucleoprotein (np), 396-404. we have used ctl clones that recognized only gp1, gp2, and np to sel ... | 1995 | 7645248 |
| isolation, identification, and plaque titration of parvovirus from muscovy ducks in japan. | muscovy ducks (cairina moschata) showed abnormal feathering, leg weakness, and high mortality. a virus was isolated from these ducks after several blind passages in embryonating muscovy duck eggs. the isolate was resistant to chloroform, to ph 3.2, and to 65 c for 30 min. electron microscopy showed that the isolate was an icosahedral and nonenveloped virus 20-22 nm in diameter. the isolate reacted with an antiserum against a goose parvovirus in agar gel precipitation tests. after 15 passages of ... | 1994 | 7702515 |
| current approaches to the development of vaccines against disease caused by respiratory syncytial virus (rsv) and parainfluenza virus (piv). a meeting report of the who programme for vaccine development. | the paramyxoviruses respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) are the two most common agents of severe lower respiratory tract disease in infants and children throughout the world. rsv causes yearly epidemics of bronchiolitis and pneumonia in infants and young children, while piv3 is a common cause of bronchiolitis, pneumonia and croup. together these two agents account for up to 30% of all hospitalizations of infants and young children for respiratory tract disease ... | 1995 | 7793140 |
| absence of varicella-zoster virus (vzv) glycoprotein v does not alter growth of vzv in vitro or sensitivity to heparin. | varicella-zoster virus (vzv) encodes at least five glycoproteins, gpi to gpv. vzv gpv, m(r) 100k to 110k, is the product of vzv open reading frame (orf) 14. vzv gpv is homologous to herpes simplex virus gc and pseudorabies virus giii. to determine whether gpv is required for viral replication, we inserted a stop codon after the fifteenth codon of the orf14 gene in a cosmid containing the gene. transfection of human melanoma cells with the cosmid containing the mutant orf14 gene and three other c ... | 1994 | 7964618 |
| an outbreak of goose parvovirus infection in japan. | in a muscovy duck breeding-growing farm in aomori prefecture, most of ducklings hatched during spring in 1994 died within two-week-old. the mortality was nearly 100%. in most cases, birds died without clinical signs and some with leg weakness. by serological and virological tests, the outbreak was identified as a goose parvovirus infection. in pathological test, however, no typical manifestations of goose parvovirus infections (hepatitis and intranuclear inclusion bodies in hepatic cells) were d ... | 1995 | 8519919 |
| effectiveness of an inactivated goose parvovirus vaccine in muscovy ducks. | with goose parvovirus (gpv) strain ih, an inactivated vaccine was prepared from allantoic fluid of embryonating muscovy duck eggs inoculated with gpv. the response to vaccination was measured by virus neutralizing antibody titer against gpv. offsprings from the vaccinated flock were introduced in a farm in which gpv infection had been experienced and examined for resistance to exposure to gpv. the results showed that the intramuscular vaccination to muscovy ducks at any age stimulated significan ... | 1995 | 8720055 |
| detection of goose parvovirus genome by polymerase chain reaction: distribution of goose parvovirus in muscovy ducklings. | a portion of the goose parvovirus (gpv) genome dna was cloned, sequenced, and primers for polymerase chain reaction (pcr) were prepared. the specificity of the clone was checked by southern blot hybridization to gpv genome and by in situ hybridization with gpv infected cells. in muscovy ducklings experimentally infected with the virulent gpv strain ih, the viral genome was detected by pcr on and after 2 days in many tissues including the brain. using pcr, the primers also detected another gpv st ... | 1996 | 8806184 |
| evaluation of different serological tests for the diagnosis of goat pox using soluble antigens. | a reverse-phase passive haemagglutination (rpha) test was developed and standardised after coupling glutaraldehyde- and tannic acid-treated sheep erythrocytes with the soluble goat pox virus (gpv) antigens for the detection of gpv antibody in goat sera. the rpha test was as sensitive as the latex agglutination (la) test, and more sensitive than precipitation tests viz. agar gel precipitation and counter immunoelectrophoresis. nevertheless, the la test was practically the most useful. | 1997 | 9493297 |
| detection of goose and muscovy duck parvoviruses using polymerase chain reaction-restriction enzyme fragment length polymorphism analysis. | by using primers (al18f2 and al18r2) designed from goose parvovirus (gpv) strain ihc, an 806-bp band was amplified by polymerase chain reaction (pcr) from all of 17 samples from thailand. specificity to gpv was confirmed by southern hybridization. with restriction enzyme digestion of the pcr products, two isolates differed from the other 15 isolates by the absence of restriction sites for hincii and bglii and the presence of ecor1 site. nucleotide sequence analysis of the pcr products from the d ... | 1998 | 9533090 |
| distribution of attenuated goose parvoviruses in muscovy ducklings. | with a polymerase chain reaction (pcr) method, goose parvovirus (gpv) dna was detected in muscovy ducklings inoculated with attenuated gpv strains, ih and ihc. strain ih that had been passed 20 times in muscovy duck embryos could be detected in ducklings at 2- to 28-days after oral inoculation by pcr, however, a cell culture adapted strain ihc that had been passed 15 times in muscovy duck embryos and then successively 50 times in muscovy duck embryo fibroblasts could not be detected by 6 days po ... | 1998 | 9560783 |
| dna-based immunization for exploring the enlargement of immunological cross-reactivity against the lyssaviruses. | dna-based immunization was used for studying the cross-reactivity of lyssavirus neutralizing antibodies and for exploring the induction of a wider range of protection against lyssaviruses. in order to immunize mice with homogeneous and chimeric genes of glycoproteins (g) from two divergent lyssaviruses, we used for the first time a new plasmid (pci-neo) known to be a highly efficient vector for in vitro expression. the homogeneous plasmids pgpv and pgmok encoded the pasteur virus (pv: genotype 1 ... | 1998 | 9607065 |
| comparison of the coat protein, movement protein and rna polymerase gene sequences of australian, chinese, and american isolates of barely yellow dwarf virus transmitted by rhopalosiphum padi. | barely yellow dwarf luteovirus-gpv (bydv-gpv) is a common problem in chinese wheat crops but is unrecorded elsewhere. a defining characteristic of gpv is its capacity to be transmitted efficiently by both schizaphis graminum and rhopaloshiphum padi. this dual aphid species transmission contrasts with those of bydv-rpv and bydv-sgv, globally distributed viruses, which are efficiently transmitted only by rhopaloshiphum padi and schizaphis graminum respectively. the viral rna sequences encoding the ... | 1998 | 9645205 |
| serological survey of viral pathogens in bean and white-fronted geese from germany. | sera from wild geese were tested for antibodies to selected viral pathogens at a resting site for wild waterfowl in germany. serum samples from both bean geese (anser fabalis) and white-fronted geese (anser albifrons) collected in october 1991 were examined using serological methods licensed for routine diagnosis in domestic poultry. of 130 sera tested, antibodies to several infectious agents were found including newcastle disease virus (45%), goose parvovirus (48%), avian reovirus (29%), and av ... | 1998 | 9706557 |
| [the serologic detection of virus-induced infections in the domestic goose (anser anser dom.)]. | the most important virus-induced diseases associated with heavy losses in the domestic goose are derzsy's disease which is caused by a goose parvovirus and duck plague (duck viral enteritis) which is caused by an avian herpesvirus. both diseases still occur but can be prevented by timely vaccinations. antibodies against influenza a viruses of the subtypes h1, h5, and h7 as well as against avian paramyxoviruses of the serogroups 4, 6, and 8, respectively, were not detected in any of the examined ... | 1998 | 9710927 |
| genetic characterization of parainfluenza virus 3 derived from guinea pigs. | to understand the relationship between novel parainfluenza virus 3 (piv-3), which has recently been isolated from the lungs of guinea pigs, and other piv-3 strains, we determined the complete nucleotide sequence of the novel piv-3 (gpv) genome. a comparison of the nucleotide sequence among piv-3s, including bovine piv-3, revealed that gpv is closely related to human piv-3. the results of the phylogenetic analysis clearly showed that gpv is a lineage of human piv-3, suggesting that gpv has probab ... | 1998 | 9764404 |
| comparative characterization of rep proteins from the helper-dependent adeno-associated virus type 2 and the autonomous goose parvovirus. | adeno-associated viruses (aavs) are nonautonomous human parvoviruses in that they are dependent on helper functions supplied by other viruses or on genotoxic stimuli for conditions permissive for replication. in the absence of helper, aav type 2 enters latency by integration into a specific site on human chromosome 19. this feature of aav, in combination with a lack of pathogenicity, makes aav an attractive candidate vector for human gene therapy. goose parvovirus (gpv) is both autonomous and pa ... | 1999 | 10074142 |
| fluorescent and electron-microscopy immunoassays employing polyclonal and monoclonal antibodies for detection of goose parvovirus infection. | polyclonal antibodies (pabs) raised in geese and eight mice hybridomas secreting monoclonal antibodies (mabs) against the goose parvovirus (gpv) were prepared. they were used for development of immunofluorescence (if) and immunoelectron-microscopic (iem) techniques to demonstrate the gpv infection in infected organs and biological fluids. the gpv antigens were established by immunofluorescence within the nuclei and the cytoplasm of many infected cells of the chorioallantoic membrane of goose and ... | 1999 | 10328532 |
| a capripoxvirus detection pcr and antibody elisa based on the major antigen p32, the homolog of the vaccinia virus h3l gene. | sheeppoxvirus (spv), goatpoxvirus (gpv) and lumpy skin disease virus (lsdv) of cattle belong to the capripoxvirus genus of the poxviridae family and can cause significant economic losses in countries where they are endemic. capripox diagnosis by classical virological methods dependent on live capripox virus is not suitable in countries such as australia where the virus is exotic and live virus is not available. to develop diagnostic tests based on recombinant material, we cloned and sequenced a ... | 1999 | 10485266 |
| expression of goose parvovirus vp1 capsid protein by a baculovirus expression system and establishment of fluorescent antibody test to diagnose goose parvovirus infection. | the gene encoding vp1 capsid protein of a goose parvovirus was cloned into a baculovirus transfer vector and a recombinant baculovirus was produced. the recombinant virus expressed a protein of 88 kda corresponding to the molecular weight of vp1 protein and the protein was detected by immunoblotting. by indirect fluorescent antibody (ifa) test, the expressed protein was detected in the nucleus of the insect cells as big granules and electron microscopy also showed several big granules in the nuc ... | 1999 | 10486117 |
| evidence of muscovy duck parvovirus in muscovy ducklings in california. | muscovy duck parvovirus (mdpv) has been demonstrated in tissue samples from one- to four-week-old commercially reared muscovy ducks that were weak, unable to walk and had a high mortality rate. on postmortem examination, the thigh and leg muscles, and the myocardium were found to be pale, and there was a fibrinous exudate on the capsule of the liver, and ascites. the parvovirus was isolated in embryonated muscovy duck eggs and visualised by negative stain electron microscopy, detected by polymer ... | 2000 | 10674693 |
| [molecular cytogenetic characterization of a new wheat line yw443 with resistance to barley yellow dwarf virus]. | a new wheat line yw443 with bydv resistance and good traits was developed from the combination of pp9-1/shan7859¿fengkang 8. the wheat line yw443 was identified by bydv resistance analysis, genomic in situ hybridization (gish), rflp and rapd analysis. the results indicated as follows: (1) yw443 was resistant to gpv and gav strains of bydv; (2) yw443 is a homozygous wheat-thinopyrum intermedium translocation line with a pair of bydv resistance genes; (3) the 7stl segment of th. intermedium carrin ... | 2000 | 11051722 |
| amino-terminal domain exchange redirects origin-specific interactions of adeno-associated virus rep78 in vitro. | the unique ability of adeno-associated virus type 2 (aav) to site-specifically integrate its genome into a defined sequence on human chromosome 19 (aavs1) makes it of particular interest for use in targeted gene delivery. the objective underlying this study is to provide evidence for the feasibility of retargeting site-specific integration into selected loci within the human genome. current models postulate that aav dna integration is initiated through the interactions of the products of a singl ... | 2001 | 11238849 |
| genetic variation of the nucleocapsid genes of waterfowl parvovirus. | duck parvovirus (dpv) and goose parvovirus (gpv) isolated from infected waterfowls with derzsy's disease in the year 1999 were identified by polymerase chain reaction and sequencing. the nucleotide sequences of their viral capsid proteins (vps) show that they share 77% similarity at the dna, and 84.6% at the protein level. the most variable region between dpv and gpv resides in the n-terminal of vp2 before the initiation codon of vp3 with 35% (19/54) amino acids divergence. viral capsid protein ... | 2001 | 11767048 |
| sequence analysis of f gene of sf02 isolate of goose paramyxovirus and its identification by multiplex rt-pcr. | the complete f gene of sf02 of goose paramyxovirus (gpv) has been cloned and analyzed. the sequence analysis demonstrated that the f gene of sf02 contains 1 662 nt and encodes 553 amino acids, and its cleavage activation site of f gene has the same deduced amino acid sequence, (112)r-r-q-k-r-f(117), as the velogenic (highly pathogenic) strain of newcastle disease virus. the latter correlated with the virulence of the isolate in biological assays. the f gene of sf02 isolate with the domestic stan ... | 2002 | 12098765 |
| differential stability and fusion activity of lyssavirus glycoprotein trimers. | the oligomeric structure and the fusion activity of lyssavirus glycoprotein (g) was studied by comparing g from mokola virus (gmok) and rabies virus (pv strain) (gpv), which are highly divergent lyssaviruses. g expressed at the surface of bsr cells upon either plasmid transfection or virus infection are shown to be mainly trimeric after cross-linking experiments. however, solubilization by a detergent (chaps) and analysis in sucrose sedimentation gradient evidenced that gmok trimer is less stabl ... | 2003 | 12573496 |
| development of a rapid, sensitive and specific diagnostic assay for fish aquareovirus based on rt-pcr. | a rapid, sensitive and highly specific detection method for aquareovirus based on reverse-transcription polymerase chain reaction (rt-pcr) was developed. based on multiple sequence alignment of the cloned sequences of a local isolates, the threadfin reovirus (tfv) and guppy reovirus (gpv) with grass carp reovirus (gcrv), a pair of degenerate primers was selected carefully and synthesized. using this primer combination, only one specific product, approximately 450 bp in length was obtained when r ... | 2004 | 15081606 |
| differentiation of sheep pox and goat poxviruses by sequence analysis and pcr-rflp of p32 gene. | sheep pox and goat pox are highly contagious viral diseases of small ruminants. these diseases were earlier thought to be caused by a single species of virus, as they are serologically indistinguishable. p32, one of the major immunogenic genes of capripoxvirus, was isolated and sequenced from two indian isolates of goat poxvirus (gpv) and a vaccine strain of sheep poxvirus (spv). the sequences were compared with other p32 sequences of capripoxviruses available in the database. sequence analysis ... | 2004 | 15215685 |
| goose parvovirus in england and wales. | | 2004 | 15328745 |
| phylogenetic analysis of hungarian goose parvovirus isolates and vaccine strains. | polymerase chain reaction and sequencing were used to analyse goose parvovirus field isolates and vaccine strains. two fragments of the genome were amplified. fragment "a" represents a region of vp3 gene, while fragment "b" represents a region upstream of the vp3 gene, encompassing part of the vp1 gene. in the region of fragment "a" the deduced amino acid sequence of the strains was identical, therefore differentiation among strains could be done only at the nucleotide level, which resulted in t ... | 2004 | 15370042 |
| characterization of a semi-replicative gene delivery system allowing propagation of complementary defective retroviral vectors. | recently, several cancer gene therapy studies have shown that replication-competent retroviral vectors represent a major improvement over replication-defective ones in terms of transgene propagation efficiency. however, this positive effect is somewhat spoiled by the increased risk of dissemination and oncogenesis that replication-competent retroviral vectors entail. to enhance both their integral safety and their transgene capacity, we developed a semi-replication-competent retroviral vector sy ... | 2005 | 15515136 |
| genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives. | to understand the genetic variations between the field strains of waterfowl parvoviruses and their attenuated derivatives, we analyzed the complete nucleotide sequences of the viral protein 1 (vp1) genes of nine field strains and two vaccine strains of waterfowl parvoviruses. sequence comparison of the vp1 proteins showed that these viruses could be divided into goose parvovirus (gpv) related and muscovy duck parvovirus (mdpv) related groups. the amino acid difference between gpv- and mdpv-relat ... | 2004 | 15529973 |
| targeting platelet gpibalpha transgene expression to human megakaryocytes and forming a complete complex with endogenous gpibbeta and gpix. | bernard-soulier syndrome (bss) is a severe congenital platelet disorder that results from a deficiency of the platelet membrane glycoprotein (gp) ib/ix complex that is composed of four subunits (gpibalpha, gpibbeta, gpix, and gpv). mutations in either gpibalpha, gpibbeta, or gpix can result in bss with many of the known mutations occurring in gpibalpha. in this study, we have developed a gene therapy strategy to express hemagglutinin (ha)-tagged gpibalpha in megakaryocytes and potentially correc ... | 2004 | 15550031 |
| comparative pathological studies on domestic geese (anser anser domestica) and muscovy ducks (cairina moschata) experimentally infected with parvovirus strains of goose and muscovy duck origin. | parvovirus infection of muscovy ducks caused by a genetically and antigenically distinct virus has been reported from germany, france, israel, hungary, some asian countries and the usa. the pathological changes include those of degenerative skeletal muscle myopathy and myocarditis, hepatitis, sciatic neuritis and polioencephalomyelitis. in the study presented here, day-old and 3-week-old goslings and muscovy ducks were infected experimentally with three different parvovirus strains (isolates of ... | 2005 | 15782661 |
| isolation and identification of goose parvovirus in the uk. | | 2005 | 15816202 |
| adeno-associated virus type 2 enhances goose parvovirus replication in embryonated goose eggs. | the autonomous goose parvovirus (gpv) and the human helper-dependent adeno-associated virus type 2 (aav2) share a high degree of homology. to determine if this evolutionary relationship has a biological impact, we studied viral replication in human 293 cells and in embryonated goose eggs coinfected with both viruses. similar experiments were performed with the minute virus of mice (mvm), an autonomous murine parvovirus with less homology to aav2. in human 293 cells, both gpv and mvm augmented aa ... | 2005 | 15892967 |
| the expression strategy of goose parvovirus exhibits features of both the dependovirus and parvovirus genera. | the rna transcription profile of the goose parvovirus (gpv) was determined, and it is a surprising hybrid of features of the parvovirus and dependovirus genera of the parvovirinae subfamily of the parvoviridae. similar to the dependovirus adeno-associated virus type 5, rnas transcribed from the gpv upstream p9 promoter, which encode the viral nonstructural proteins, were polyadenylated at a high efficiency at a polyadenylation site [(pa)p] located within an intron in the center of the genome. ef ... | 2005 | 16103154 |
| expression of capsid proteins and non-structural proteins of waterfowl parvoviruses in escherichia coli and their use in serological assays. | while there are a number of methods available for detection of antibodies against waterfowl parvoviruses, none is able to differentiate responses against the capsid and non-structural proteins. to enable this, the capsid and non-structural proteins of goose parvovirus (gpv) and muscovy duck parvovirus (mdpv) were expressed in escherichia coli. these proteins were purified and used as antigens in western blotting assays of antibodies against gpv and mdpv. the results showed that 94.7% of the goos ... | 2005 | 16236567 |
| the current status of sheep pox disease. | sheep are the moving banks of shepherds and their economic contribution in terms of meat, wool and skin/hide is immense. various infectious diseases jeopardize the optimum productivity; among which sheep pox is more important as the disease restricts the export of sheep and their products besides other economic losses. although, clinical signs are indicative of the disease but a laboratory confirmation is necessary for unequivocal diagnosis and studying epidemiology. the causative agent, sheep p ... | 2006 | 16458357 |
| serological survey of racing pigeons for selected pathogens in taiwan. | to investigate the pathogens that racing pigeons in taiwan are exposed to, a total of 3764 pigeons from 90 lofts were analysed by collection of blood samples in the period between october 2000 and september 2001. the haemagglutination inhibition (hi) test was performed to detect antibodies against newcastle disease virus (ndv), type 2 avian paramyxovirus (apmv-2), and egg drop syndrome '76 virus (eds-76v). the agar-gel precipitin (agp) test was used to detect antibodies against fowl adenovirus ( ... | 2006 | 16841756 |
| platelet autoantibodies are common in hepatitis c infection, irrespective of the presence of thrombocytopenia. | to investigate the generation of platelet antibodies in hepatitis c virus (hcv)-infected individuals and their relation to the development to thrombocytopenia with the aim of using their detection as a diagnostic aid of immune thrombocytopenia in these patients. | 2006 | 17042765 |
| isolation and molecular characterization of a new muscovy duck parvovirus from muscovy ducks in the usa. | between 1997 and 1999 several cases of a new disease in muscovy ducks were reported in pennsylvania, usa. the cases were characterized by locomotor dysfunction, weakness, recumbency, 40 to 60% morbidity and 10 to 40% mortality. the most characteristic microscopic lesions were moderate to severe degenerative rhabodomyopathy. in order to characterize the aetiological agent, virus isolation was attempted from the spleen, liver, heart, skeletal muscle and intestine by inoculation of 14-day-old musco ... | 2006 | 17121731 |
| [transgenic wheat expressing virus-derived hairpin rna is resistant to barley yellow dwarf virus]. | an expression vector expected to induce rna interference against barley yellow dwarf virus (bydv), which expressed a composite hprna with the dsrna stem homologous of bydv gpv replicase gene and the antisense rna loop homologous of coat protein gene, was designed without marker gene. the vector was transferred into callus cells from wheat (triticum aestivum l.) immature embryos by particle bombardment. to select the positive transformants as early as possible, a rapid pcr, which does not need ex ... | 2007 | 17284432 |
| three digoxigenin-labeled cdna probes for specific detection of the natural population of barley yellow dwarf viruses in china by dot-blot hybridization. | three digoxigenin-labeled cdna probes complementary to the coat protein (cp) and read-through protein gene sequences of barley yellow dwarf virus - one each for three species, namely bydv-gav, gpv, and pav - were synthesized for developing a specific and sensitive dot-blot hybridization detection assay for total rna extracts from field-infected wheat plants. the sensitivity limit for bydv-gav, gpv, and pav probes corresponded to 25microg, 31.25microg, and 62.5microg tissue/spot, respectively. th ... | 2007 | 17561274 |
| epidemiologic investigation of an outbreak of goose parvovirus infection in sweden. | an outbreak of goose parvovirus (gpv) infection on a swedish goose farm in the spring of 2004 increased the mortality rates from 2% in the early unaffected hatches to 90% and 99% respectively in the two hatches following virus introduction and 40% in goslings hatched later in the same breeding season. in this paper we describe the clinical observations, diagnostic procedures, and epidemiologic investigation carried out to elucidate the source of the infection. the diagnosis was confirmed by sero ... | 2007 | 17626494 |
| use of elisa procedures for the detection of derzsy's disease virus of geese and of antibodies produced against it. | we have developed a double antibody sandwich (antigen capture) elisa for the detection of derzsy's disease virus (gpv-1) antigen produced in cell cultures. this provided unambiguous quantification of virus whereas evaluation of cytopathic effect was sometimes difficult. a blocking elisa was developed to quantify anti-gpv-1 antibodies in goose sera. this method was rapid, simple, easily standardized and correlated well with a virus neutralization test. | 1996 | 18645834 |
| additional data on mycoplasma disease of goslings. | epidemiological, pathomorphological, histological and microbiological studies on 272 gosling from 51 flocks showed that disease occurred generally at 3 to 4 weeks of age with morbidity of 15 to 25% and mortality of 3 to 8%. it was characterized by respiratory and nervous symptoms accompanied by serous-fibrinous inflammation of the mucosal membrane of the respiratory tract, pericardium and cerebral membranes. mycoplasmas of 1220 type were isolated from trachea and airsac walls of 50% of the sampl ... | 1993 | 18671005 |
| characteristics of the genome of goose parvovirus. | the nucleic acid of goose parvovirus showed sensitivity to dnase and mung bean nuclease treatment and resistance to digestion with rnase. viral dna readily served as a template for self-primed conversion in vitro into a double-stranded form of about 5000 base pairs. there was evidence for encapsidation of strands of opposite polarities in equal amounts. the restriction enzyme cleavage patterns of goose and muscovy duck parvovirus dnas differed significantly. however, they showed a high degree of ... | 1994 | 18671101 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 18706944 |
| persistence of parvovirus antibody in geese that have survived derzsy's disease. | virus neutralisation (vn) and agar gel precipitin (agp) tests were used to detect and measure parvovirus antibodies in geese that had survived derzsy's disease and susceptible age-matched contacts kept under laboratory conditions. serum samples were collected over a period of 80 months and tested for the presence of parvovirus antibodies. between 68 and 72 months, eggs and progeny were tested for the presence of antibodies and resistance to challenge. high and persistent levels of antibody were ... | 1987 | 18766618 |
| [results of a serological survey made in france (vendeé region) in domestic ducks]. | the sera of domestic ducks were examined for antibodies to several infectious agents of palmipeds during the winter of 1982 in the abattoirs in la vendée, an important region of duck production in the west of france. the performance of each batch and their antecedents was also studied. in barbary ducks and crossbred ducks (from male wild ducks and female domestic ducks), antibodies were found to the virus of egg drop syndrome 16 (eds 76), to newcastle disease virus (ndv), to duck hepatitis virus ... | 1983 | 18766801 |
| application of the agar gel precipitin and virus neutralisation tests to the serological study of goose parvovirus. | an agar gel precipitin (agp) test and virus neutralisation (vn) test were developed using antigens prepared in duck eggs, to detect and measure antibodies against goose parvovirus. the tests were evaluated using sera collected from the following sources; forty three-month-old geese that had survived derzsy's disease as goslings, adult geese from a farm where an outbreak of derzsy's disease had occurred in goslings 14 months earlier, geese that had received a duck embryo adapted goose parvovirus ... | 1984 | 18766864 |
| failure of orally administered attenuated goose parvovirus strain b to induce a humoral immune response in adult geese. | two-month-old geese responded with the production of virus neutralising antibodies against virulent goose parvovirus strain b administered either per os or intramuscularly. they were shedding the virus within a short period after exposure. humoral immune response in geese of the same age was induced by the attenuated goose parvovirus strain b only by intramuscular injection but not with per os administration. | 1981 | 18770126 |
| studies with a duck embryo adapted goose parvovirus vaccine. | a goose parvovirus isolated from goslings with derzsy's disease was adapted to growth in embryonating duck eggs. after 22 passages the adapted parvovirus was evaluated in 1 to 3-day-old susceptible goslings for attenuation and immunogenicity. the response to vaccination was measured by virus neutralisation (vn) tests and resistance to experimental challenge. the results showed that the duck embryo adapted parvovirus vaccine was apathogenic for goslings and stimulated significant vn antibody leve ... | 1982 | 18770212 |
| immunological aspects of derzsy's disease in goslings. | laying geese naturally infected by the parvovirus agent of derzsy's disease transmit igg type maternal immunoglobulins through the eggs to their offsprings. the passively acquired yolk antibodies persisted at a relatively high level until about 12-14 days of age, but were eliminated from the birds during the 3rd week of life. the igg type immunoglobulins present in the specific hyperimmune sera used for preventive treatment have a half-life, about 6 days, and their blood level falls to a minimal ... | 1977 | 18770342 |
| attenuation of the goose parvovirus strain b. laboratory and field trials of the attenuated mutant for vaccination against derzsy's disease. | serial transfer of the goose parvovirus strain b, causal agent of derzsy's gosling disease, in cultured goose-embryo fibroblast (gef) resulted in a mutant (designated as bav) apathogenic for both goose embryos and susceptible goslings. goose embryos inoculated with the 38th or higher passages of strain b survived the infection, although the virus replicated in their organs. susceptible goslings survived challenge with the bav strain without showing symptoms, and developed normally. only 4.2% of ... | 1978 | 18770393 |
| cross-neutralization tests on parvoviruses isolated from goslings. | the hungarian, french and dutch goose parvovirus isolates proved to be closely related to each others in the cross-neutralization test performed lin tissue culture system. the results differed from those obtained earlier in embryonated goose eggs and gosling-protection test. | 1974 | 18777284 |
| mycoplasma infection of geese. ii. studies on pathogenicity of mycoplasmas in goslings and goose and chicken embryos. | of various mycoplasma strains of goose original, axanthum strains (609 and 612) caused the death both of goose and chicken embryos, a. laid-lawii strain (606) killed only goose embryos, whereas m. gallinarum (598) failed to kill either. infection of 3-day-old goslings with these mycoplasmas resulted in no mortality but lesions were produced with a. axanthum in 9 of 10 birds. less severe lesions were seen in fewer birds infected with other strains. dual infection of 3-day-old goslings, with mater ... | 1976 | 18777325 |
| identification of sequence changes in live attenuated goose parvovirus vaccine strains developed in asia and europe. | live attenuated vaccines have been used for control of the disease caused by goose parvovirus (gpv), but the mechanism involved in attenuation of gpv remains elusive. this report presents the complete nucleotide sequences of two live attenuated strains of gpv (82-0321v and vg32/1) that were independently developed in taiwan and europe, together with the parental strain of 82-0321v and a field strain isolated in taiwan in 2006. sequence comparisons showed that 82-0321v and vg32/1 had multiple del ... | 2008 | 18798024 |
| goose parvovirus in great britain. | | 2008 | 18849581 |
| formulation of a protein-free medium based on ipl-41 for the sustained growth of drosophila melanogaster s2 cells. | an animal protein-free medium was developed for drosophila melanogaster s2 (s2acgpv2) cells genetically modified to produce the rabies virus g glycoprotein (gpv). ipl-41, used as a basal medium, was supplemented with yeastolate, carbohydrates, amino acids and lipids aiming initially to reduce and further to eliminate the need of fetal bovine serum. the s2acgpv2 cells were fully capable of growing in serum-free supplemented ipl-41 medium containing 6 g l(-1) yeastolate ultrafiltrate, 10 g l(-1) g ... | 2008 | 19003168 |
| insect cells respiratory activity in bioreactor. | specific respiration rate ( [formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. in this work, we cultivated different insect cells in a very controlled environment, being able to measure [formula: see text]. spodoptera frugiperda (sf9) cells have been used through virus infection as host for foreign protein expression and bioinsecticide production. transfected drosophila melanogaster (s2) ... | 2008 | 19003170 |
| phylogenetic analysis of parvoviruses isolated in taiwan from ducks and geese. | two major outbreaks of parvovirus infection occurred in domestic waterfowls in taiwan in the last two decades; the first was in 1982 and the second in 1989/1990. parvoviruses isolated in the two outbreaks were sequenced between nucleotides 142 and 680 of the vp3 gene. sequence comparisons reveal that these viruses could be divided into two groups respectively related to goose parvovirus (gpv) and muscovy duck parvovirus (mdpv). nucleotide differences between ''gpv'' and ''mdpv'' groups range fro ... | 2000 | 19184788 |
| short beak and dwarfism syndrome of mule duck is caused by a distinct lineage of goose parvovirus. | from the early 1970s to the present, numerous cases of short beak and dwarfism syndrome (sbds) have been reported in mule ducks from france. the animals showed strong growth retardation with smaller beak and tarsus. it was suggested that the syndrome was caused by goose parvovirus on the basis of serological investigation, but the causative agent has not been isolated and the disease has not so far been reproduced by experimental infection. the aim of the present study was to characterize the vi ... | 2009 | 19322718 |
| multicolor quantum dot-encoded microspheres for the fluoroimmunoassays of chicken newcastle disease and goat pox virus. | semiconductor nanocrystals (or quantum dots, qds) have the potential to overcome some of the limitations encountered by traditional fluorophores in fluorescence labeling applications. the unique spectroscopic properties of qds make them hold immense promise as versatile labels for biological applications. in this work, we employ the layer-by-layer (lbl) method for the construction of bio-functional multicolor qd-encoded microspheres. polystyrene microspheres with diameter of 3 microm were used a ... | 2009 | 19452974 |
| development and evaluation of an antigen-capture elisa for detection of the ul24 antigen of the duck enteritis virus, based on a polyclonal antibody against the ul24 expression protein. | an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) method was developed for the efficient detection of the ul24 antigen of the duck enteritis virus (dev) using polyclonal antibodies. ducks and rabbits were immunized, respectively, with expressed ul24 recombinant protein. the igg antibodies against ul24 from ducks and rabbits were purified and used as the capture antibodies. the specificity of the optimized ac-elisa was evaluated by use of dev, duck hepatitis virus (dhv), duck hepati ... | 2009 | 19467266 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 19475729 |
| the complete nucleotide sequence of the barley yellow dwarf gpv isolate from china shows that it is a new member of the genus polerovirus. | the complete nucleotide sequence of the ssrna genome of a chinese gpv isolate of barley yellow dwarf virus (bydv) was determined. it comprised 5673 nucleotides, and the deduced genome organization resembled that of members of the genus polerovirus. it was most closely related to cereal yellow dwarf virus-rpv (77% nt identity over the entire genome; coat protein amino acid identity 79%). the gpv isolate also differs in vector specificity from other bydv strains. biological properties, phylogeneti ... | 2009 | 19551470 |
| [development and application of taqman-mgb real-time quantitative pcr assay for detection of goat pox virus]. | the complete gene sequences of eight capripoxvirus strains in genbank were aligned and analyzed with dnastar software. we selected a size of 64 bp gene fragment that was located in gp064 region of goat pox virus (gpv) genome, and designed a pair of primers and a taqman-mgb probe against the gene fragment with primer express 2.0 software. then, the fluorescence quantitative pcr (fq-pcr) assay was developed and the standard curve of different dilution series was described. we extracted the dna sam ... | 2009 | 19621592 |
| the choice of translation initiation site of the rep proteins from goose parvovirus p9-generated mrna is governed by splicing and the nature of the excised intron. | the goose parvovirus (gpv) rep 1 and rep 2 proteins are encoded by p9-generated mrnas that are either unspliced or spliced within the rep gene region, respectively. these mrnas are present in an approximately equal ratio. the translation of rep 1 was initiated from the first aug in unspliced p9-generated mrna; however, this aug was bypassed in spliced p9-generated rna and rep 2 translation initiated predominately at the next initiating aug downstream. we show that the choice of the site of initi ... | 2009 | 19625410 |
| [recombinant goat pox virus expressing pprv h protein]. | the purpose of the study is to construct recombinant goat pox virus (gpv) expressing peste des petits ruminants virus (pprv) h protein, and to evaluate the immunization effect. recombinant gpv containing pprv h gene (rgpv-pprv-h) was selected and purified by gpt and egfp utilizing plaque purification, and the final selected recombinant gpv was proved to be purified by pcr. immunofluorescence and western blotting showed that the recombinant virus could express h protein of pprv while infecting la ... | 2009 | 19637621 |