| extracting single genomes from heterogenous dna samples: a test case with carsonella ruddii, the bacterial symbiont of psyllids (insecta). | analysis of many bacterial genomes is impeded by the inability to separate individual species from complex mixtures of cells or to propagate cells in pure culture. this problem is an obstacle to the study of many bacterial symbionts that live intracellularly in insects and other animals. to recover bacterial dna from complex samples, we devised a method that facilitates the cloning of dna fragments of distinctive g+c contents in order to generate shotgun dna libraries enriched in inserts having ... | 2005 | 16299593 |
| genome size of pachypsylla venusta (hemiptera: psyllidae) and the ploidy of its bacteriocyte, the symbiotic host cell that harbors intracellular mutualistic bacteria with the smallest cellular genome. | psyllids harbor the primary symbiont, carsonella ruddii (gamma-proteobacteria), within the cytoplasm of specialized cells called bacteriocytes. carsonella has the smallest known cellular genome (160 kb), lacking numerous genes that appear to be essential for bacterial life. this raises the question regarding the genetic mechanisms of the host which supports the survival of carsonella. our preceding analyses have indicated that some of the genes that are encoded in the psyllid genome and which ar ... | 2010 | 19302725 |
| parallel histories of horizontal gene transfer facilitated extreme reduction of endosymbiont genomes in sap-feeding insects. | bacteria confined to intracellular environments experience extensive genome reduction. in extreme cases, insect endosymbionts have evolved genomes that are so gene-poor that they blur the distinction between bacteria and endosymbiotically derived organelles such as mitochondria and plastids. to understand the host's role in this extreme gene loss, we analyzed gene content and expression in the nuclear genome of the psyllid pachypsylla venusta, a sap-feeding insect that harbors an ancient endosym ... | 2014 | 24398322 |
| the complete mitochondrial genome sequence of bactericera cockerelli and comparison with three other psylloidea species. | potato psyllid (bactericera cockerelli) is an important pest of potato, tomato and pepper. not only could a toxin secreted by nymphs results in serious phytotoxemia in some host plants, but also over the past few years b. cockerelli was shown to transmit "candidatus liberibacter solanacearum", the putative bacterial pathogen of potato zebra chip (zc) disease, to potato and tomato. zc has caused devastating losses to potato production in the western u.s., mexico, and elsewhere. new knowledge of t ... | 2016 | 27227976 |