| isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (cvmv) promoter. | the cassava vein mosaic virus (cvmv) is a double stranded dna virus which infects cassava plants (manihot esculenta crantz) and has been characterized as a plant pararetrovirus belonging to the caulimovirus subgroup. two dna fragments, cvp1 of 388 nucleotides from position -368 to +20 and cvp2 of 511 nucleotides from position -443 to +72, were isolated from the viral genome and fused to the uida reporter gene to test promoter expression. the transcription start site of the viral promoter was det ... | 1996 | 8914529 |
| functional organization of the cassava vein mosaic virus (csvmv) promoter. | cassava vein mosaic virus (csvmv) is a pararetrovirus that infects cassava plants in brazil. a promoter fragment isolated from csvmv, comprising nucleotides -443 to +72, was previously shown to direct strong constitutive gene expression in transgenic plants. here we report the functional architecture of the csvmv promoter fragment. a series of promoter deletion mutants were fused to the coding sequence of uida reporter gene and the chimeric genes were introduced into transgenic tobacco plants. p ... | 1998 | 9700077 |
| characterization and genomic analysis of tobacco vein clearing virus, a plant pararetrovirus that is transmitted vertically and related to sequences integrated in the host genome. | a previously undescribed caulimo-like virus was identified in the hybrid tobacco species nicotiana edwardsonii, and was named tobacco vein clearing virus (tvcv) after the symptoms associated with its occurrence in this plant. the virions of tvcv are 50 nm in diameter and are composed of a 45 kda capsid protein and a 7767 bp dsdna genome. each strand of the genome is interrupted by a site-specific discontinuity. in genome sequence and arrangement of orfs tvcv was most similar to cassava vein mosa ... | 2000 | 10811941 |
| a comparison of constitutive promoters for expression of transgenes in alfalfa (medicago sativa). | the activity of constitutive promoters was compared in transgenic alfalfa plants using two marker genes. three promoters, the 35s promoter from cauliflower mosaic virus (camv), the cassava vein mosaic virus (csvmv) promoter, and the sugarcane bacilliform badnavirus (scbv) promoter were each fused to the beta-glucuronidase (gusa) gene. the highest gus enzyme activity was obtained using the csvmv promoter and all alfalfa cells assayed by in situ staining had high levels of enzyme activity. the 35s ... | 2004 | 15517994 |
| biodegradation of atrazine in transgenic plants expressing a modified bacterial atrazine chlorohydrolase (atza) gene. | atrazine is one of the most widely used herbicides in the usa. atrazine chlorohydrolase (atza), the first enzyme in a six-step pathway leading to the mineralization of atrazine in gram-negative soil bacteria, catalyses the hydrolytic dechlorination and detoxification of atrazine to hydroxyatrazine. in this study, we investigated the potential use of transgenic plants expressing atza to take up, dechlorinate and detoxify atrazine. alfalfa, arabidopsis thaliana and tobacco were transformed with a ... | 2005 | 17173634 |
| improved expression of recombinant gfp using a replicating vector based on beet curly top virus in leaf-disks and infiltrated nicotiana benthamiana leaves. | recombinant green fluorescent protein (gfp) with a molecular mass of 29 kda was transiently expressed in agrobacterium-inoculated leaf-disks prepared from nicotiana benthamiana plants. expression of gfp from the cauliflower mosaic virus (camv) 35 s promoter within a replicating vector based on the geminivirus beet curly top virus (bctv) was more than 3 times higher than from a control, non-replicating vector. use of the cassava vein mosaic virus (csvmv) promoter in the bctv replicating vector in ... | 2007 | 17294255 |
| evaluation of constitutive viral promoters in transgenic soybean roots and nodules. | the efficiency of beta-glucuronidase (gus) expression was evaluated with five viral promoters to identify the most suitable promoter or promoters for use in soybean hairy roots, including applications to study the symbiotic interaction with bradyrhizobium japonicum. levels of gus activity were fluorimetrically and histochemically assayed when the gus (uida) gene was driven by the cauliflower mosaic virus (camv) 35s promoter and enhanced 35s (e35s) promoter, the cassava vein mosaic virus (csvmv) ... | 2008 | 18616399 |
| field level rnai-mediated resistance to cassava brown streak disease across multiple cropping cycles and diverse east african agro-ecological locations. | cassava brown streak disease (cbsd) presents a serious threat to cassava production in east and central africa. currently, no cultivars with high levels of resistance to cbsd are available to farmers. transgenic rnai technology was employed to combat cbsd by fusing coat protein (cp) sequences from ugandan cassava brown streak virus (ucbsv) and cassava brown streak virus (cbsv) to create an inverted repeat construct (p5001) driven by the constitutive cassava vein mosaic virus promoter. twenty-fiv ... | 2016 | 28127301 |
| unraveling complex viral infections in cassava (manihot esculenta crantz) from colombia. | in the americas, different disease symptoms have been reported in cassava including leaf mosaics, vein clearings, mottles, ring spots, leaf distortions and undeveloped and deformed storage roots. some viruses have been identified and associated with these symptoms while others have been reported in symptomless plants or latent infections. we observed that reoviruses associated with severe root symptoms (rs) of cassava frogskin disease (cfsd) are not associated with leaf symptoms (ls) observed in ... | 2014 | 24374265 |
| biodegradation of atrazine by three transgenic grasses and alfalfa expressing a modified bacterial atrazine chlorohydrolase gene. | the widespread use of atrazine and other s-triazine herbicides to control weeds in agricultural production fields has impacted surface and groundwater in the united states and elsewhere. we previously reported the cloning, sequencing, and expression of six genes involved in the atrazine biodegradation pathway of pseudomonas sp. strain adp, which is initiated by atza, encoding atrazine chlorohydrolase. here we explored the use of enhanced expression of a modified bacterial atrazine chlorohydrolas ... | 2015 | 25432082 |
| glyceollin is an important component of soybean plant defense against phytophthora sojae and macrophomina phaseolina. | the response of soybean transgenic plants, with suppressed synthesis of isoflavones, and nontransgenic plants to two common soybean pathogens, macrophomina phaseolina and phytophthora sojae, was studied. transgenic soybean plants of one line used in this study were previously generated via bombardment of embryogenic cultures with the phenylalanine ammonia lyase, chalcone synthase, and isoflavone synthase (ifs2) genes in sense orientation driven by the cotyledon-preferable lectin promoter (to tur ... | 2013 | 23617338 |
| expression of a bifunctional green fluorescent protein (gfp) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape (vitis vinifera). | activity of three constitutive promoters and enhanced derivatives in transgenic grape (vitis vinifera l. cv. thompson seedless) was characterized using a bifunctional fusion marker containing the enhanced green fluorescent protein (egfp) and neomycin phosphotransferase (nptii) genes. relative differences in transient gfp expression and stable transformation efficiencies were used to compare promoter activity. expression patterns in transformed somatic embryos revealed that the act2 promoter from ... | 2001 | 11297784 |
| cassava vein mosaic virus (csvmv), type species for a new genus of plant double stranded dna viruses? | the complete sequence of 8159 nucleotides of the double stranded dna genome of cassava vein mosaic virus (csvmv) was determined (# u59751) and revealed a significant difference in genome organization when compared with a previous report (# u20341). when transferred to cassava plants by microbombardment, the full length csvmv clone was infectious, confirming the genome organization here described. sequence comparisons between csvmv and members of the genera caulimovirus and badnavirus revealed hi ... | 1998 | 9645200 |
| characterization of cassava vein mosaic virus: a distinct plant pararetrovirus. | cassava vein mosaic virus (cvmv) was found to be widespread throughout the north-eastern region of brazil. the complete sequence of cvmv was determined, and the genome was 8158 bp in size. a cytosolic initiator methionine trna (trna met1)-binding site that probably acts as a primer for minus-strand synthesis was present. the genome contained five open reading frames that potentially encode proteins with predicted molecular masses of 186 kda, 9 kda, 77 kda, 24 kda and 26 kda. the putative 186 kda ... | 1995 | 7730813 |
| cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture. | this study aimed to develop a methodology for eliminating cassava frogskin disease (cfsd) from in vitro shoot tip culture by associating thermotherapy and tetracycline. cuttings from different accessions (bgm0232, bgm0315, bgm0464, bgm584, bgm0841, and bgm1342), infected with cfsd according to visual inspection of the disease symptoms, were used for cleaning. to verify the absence of other diseases, the plants were indexed for cassava common mosaic virus - cscmv (by elisa) and cassava vein mosai ... | 2017 | 28613372 |