| peptidyl transfer ribonucleic acid hydrolase activity of proteinase k. | proteinase k, a seryl-protease obtained from tritirachium album, is able to specifically hydrolyze n-blocked aminoacyl transfer ribonucleic acids (trnas). the blocked amino acid is released, and the trna molecule remains able to be recharged by its cogante amino acid. aminoacyl-trnas are highly resistant to hydrolysis by the protease. this activity is not due to contamination of the protease preparation. a commercial protease from streptomyces griseus displayed a similar activity, while trypsin, ... | 1979 | 385046 |
| heterogeneity of rabbit muscle creatine kinase and limited proteolysis by proteinase k. | by using sodium dodecyl sulphage/polyacrylamide-gel electrophoresis it was shown that rabbit muscle creatine kinase, both in a homogenate and purified, appears to be composed of a mixture of two peptides (mol.wts. 42100 and 40300) differing in length by about 15 amino acids. it is found that low concentrations of proteinase k from the fungus tritirachium album can cleave about 38 amino acids from each chain of creatine kinase, leaving two large fragments (mol.wts 37700 and 35500). scission of th ... | 1977 | 603634 |
| proteinase k from the mold tritirachium album limber. specificity and mode of action. | 1) the specificity of proteinase k towards amino acid and oligopeptide nitroanilide substrates is investigated. 2) the active center of the enzyme contains an extended binding region consisting of several subsites. an integral part of the s1-subsite are hydrophobic areas which were investigated by systematic elongation of the carbon skeleton in carboxylic acid 4-nitrophenyl esters. on the basis of these studies, a possible model of the s1-binding site is proposed. 3) kinetic parameters for the h ... | 1976 | 992572 |
| trypsin-kallikrein isoinhibitor k (type kunitz) from snails (helix pomatia). purification and characterization. | a basic proteinase inhibitor, isoinhibitor k, was purified by se-sephadex c-25 column chromatography from the mixture of acid-stable and heat-stable isoinhibitors of the snail (helix pomatia). isoinhibitor k is homogeneous in polyacrylamide gel, cellulose acetate and polyacrylamide-dodecylsulfate electrophoresis. from the electrophoretic mobility in dodecylsulfate-polyacrylamide gel and apparent molecular weight of 6500 +/- 200 was estimated. from the amino acid composition the inhibitor consist ... | 1975 | 1081050 |
| isolation and thermal stability studies of two novel serine proteinases from the fungus tritirachium album limber. | a number of serine proteinases are secreted into the culture medium when tritirachium album limber is supplied with protein as the only nitrogen source. from this population of proteinases, we have isolated two novel proteolytic enzymes, designated as proteinase r and t. we have compared the thermal stability of these two proteinases with that of subtilisin bpn' and proteinase k. both of these proteinases were thermally stable in the absence of detergents in buffers of low (4.0) and high (10.0) ... | 1991 | 1367259 |
| propeptide of carboxypeptidase y provides a chaperone-like function as well as inhibition of the enzymatic activity. | the zymogen of the vacuolar carboxypeptidase y from saccharomyces cerevisiae was purified and characterized with respect to activation as well as refolding in vitro. the purified procarboxypeptidase y has no detectable activity but can be efficiently activated by proteinase k from tritirachium album. we used this method of activation as a tool for the investigation of refolding procarboxypeptidase y in vitro. the proenzyme, denatured in 6 m guanidinium chloride, is renatured efficiently after di ... | 1991 | 1924396 |
| isolation and characterization of the gene encoding a novel, thermostable serine proteinase from the mould tritirachium album limber. | a number of proteinases are induced and secreted into the culture medium of tritirachium album limber when the nitrogen source is limited to exogenous proteins. we have constructed a cdna library using the polyadenylated rna isolated during the nutritional induction with bovine serum albumin. a full-length clone of a gene for a new proteinase (named proteinase r) was identified from this library. this clone contained sequences coding for the 108-amino-acid prepro-leader as well as for the 279-am ... | 1990 | 2077361 |
| engyodontium album endocarditis. | this is the first reported case of native valve endocarditis caused by engyodontium album. this fungus, rarely seen as a human pathogen, is separated from tritirachium species by its lack of pigmentation and from beauveria species by the presence of conidiogenous cells in whorls. | 1990 | 2380370 |
| proteinase k from tritirachium album limber. characterization of the chromosomal gene and expression of the cdna in escherichia coli. | the cdna and the chromosomal gene encoding proteinase k from tritirachium album limber have been cloned in escherichia coli and the entire nucleotide sequences of the coding region, as well as 5'- and 3'-flanking regions have been determined. the deduced primary translation product consisting of 384 amino acid residues (molecular mass = 40,231 da) contains an n-terminal region of 105 amino acids not present in the mature protein. by analogy to the evolutionary-related bacterial subtilisins and o ... | 1989 | 2645134 |
| cloning and expression of the gene encoding a novel proteinase from tritirachium album limber. | we have isolated the genomic and cdna clones encoding a novel proteinase from the fungus tritirachium album limber, named proteinase t, synthesis of which is induced in skim milk medium. the coding sequence for this enzyme is interrupted by two introns in the fungal genome. the amino acid sequence of proteinase t as deduced from the nucleotide sequence is about 53% identical to that of proteinase k. four cysteines are present in the mature proteinase, probably in the form of two disulfide bonds, ... | 1989 | 2697641 |
| x-ray and model-building studies on the specificity of the active site of proteinase k. | proteinase k, the extracellular serine endopeptidase (e.c.3.4.21.14) from the fungus tritirachium album limber, is homologous to the bacterial subtilisin proteases. the binding geometry of the synthetic inhibitor carbobenzoxy-ala-phechloromethyl ketone to the active site of proteinase k was first determined from a fourier synthesis based on synchrotron x-ray diffraction data between 1.8 a and 5.0 a resolution. the protein inhibitor complex was refined by restrained least-squares minimization wit ... | 1988 | 3237715 |
| autolysis and inhibition of proteinase k, a subtilisin-related serine proteinase isolated from the fungus tritirachium album limber. | the activity of proteinase k (ec 3.4.21.14), a subtilisin-related serine proteinase, was assayed with azoalbumin that showed non-expected behavior in substrate saturation curve because of interaction between albumin molecules. succinyl-(ala)n-p-nitroanilide with n = 2 and 3, yielded specific activities of 3.5, 13 units/mg protein, respectively, reflecting a chain length dependence. the influence of peptide chain length on binding to proteinase k was also observed using mono- and dipeptide chloro ... | 1988 | 3284591 |
| active-site geometry of proteinase k. crystallographic study of its complex with a dipeptide chloromethyl ketone inhibitor. | proteinase k (ec 3.4.21.14) from the fungus tritirachium album limber is the most active known serine endopeptidase. the sequence of its 275-residue long polypeptide chain and its three-dimensional folding show a high degree of homology with the bacterial subtilisin proteases. using difference fourier methods, the binding mode of the synthetic carbobenzoxy-ala-ala-chloromethyl ketone inhibitor to the active site of proteinase k was determined. in several cycles of restrained least-squares, the e ... | 1986 | 3512298 |
| proteinase k from tritirachium album limber. i. molecular mass and sequence around the active site serine residue. | the molecular mass of proteinase k was determined by gel electrophoresis in the presence of sodium dodecyl sulfate and by active site labelling with diisopropyl fluorophosphate. both methods indicate molecular masses in the range of 27 000-29 000 da. these values differ significantly from that of 18 500 formerly determined by gel filtration (ebeling et al. (1974) eur. j. biochem. 47, 91-97). proteinase k was inactivated with [3h]diisopropyl fluorophosphate. afterwards the labelled protein was re ... | 1985 | 3924077 |
| proteinase k from tritirachium album limber. | | 1974 | 4373242 |
| aminopeptidase k from tritirachium album limber. i. isolation and some properties. | | 1973 | 4373364 |
| aminopeptidase k from tritirachium album limber. ii. specificity and mode of action. | | 1973 | 4807837 |
| mycotic keratitis due to beauveria alba. | a 70-year-old white man developed mycotic keratitis following phacoemulsification and intraocular lens implantation. extensive therapeutic surgery was necessary to control the infection. beauveria alba, a saprophytic fungus, was cultured and demonstrated in the corneal button by histopathologic examination. | 1984 | 6100486 |
| [isolation and comparative properties of serine proteinases of the microscopic fungi trichoderma lignorum and trichoderma koningii]. | using affinity chromatography on bacitracin-sepharose combined with ion-exchange chromatography on aminosilochrome and isoelectrofocusing, individual serine proteases have been isolated for the first time from surface cultures of t. lignorum and t. koningii. the proteinases have pi values at 6.8 and 6.7 and ph optima of 10.5. both proteinases are stable within the ph range of 4-11 and have molecular weight of 21 000. the amino acid composition of t. lignorum enzyme is lys3his4arg9asx23thr17ser23 ... | 1981 | 7032610 |
| the effect of limited proteolysis on rabbit muscle creatine kinase. | creatine kinase from rabbit muscle is inactivated by limited proteolysis with proteinase k from tritirachium album. gel-filtration and cross-linking studies showed that the limited proteolysis did not affect the molecular weight of the enzyme under non-denaturing conditions, but did cause changes in the reactivity of the reactive thiol group on each subunit and in the ability of the enzyme to form a 'transition-state analogue' complex in the presence of magnesium acetate plus adp plus creatinine ... | 1981 | 7039617 |
| cloning of a cuticle-degrading protease from the entomopathogenic fungus, beauveria bassiana. | a beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. a cdna clone of the protease was isolated from mycelia of b. bassiana grown on cuticle/chitin cultures. the amino acid sequence of this gene was compared to that of metarhizium anisopliae pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase k, isolated from tritirachium album, a saprophytic fu ... | 1995 | 7875568 |
| crystal structure of calcium-free proteinase k at 1.5-a resolution. | proteinase k from the fungus tritirachium album limber binds two ca2+ ions, one strongly (ca 1) and the other weakly (ca 2). removal of these cations reduces the stability of proteinase k as shown by thermal denaturation, but the proteolytic activity is unchanged. the x-ray structures of native and ca(2+)-free proteinase k at 1.5-a resolution show that there are no cuts in the polypeptide backbone (i.e. no autolysis), ca 1 has been replaced by na+, while ca 2 has been substituted by a water asso ... | 1994 | 8083213 |
| purification and characterization of a serine proteinase from senescent sporophores of the commercial mushroom agaricus bisporus. | a proteinase has been purified from the stipes of senescent sporophores of the mushroom agaricus bisporus. the proteinase was inhibited by pmsf. it has a broad ph optimum, 6.5-11.5, and a narrow substrate specificity, requiring both a hydrophobic amino acid in the p1 position and a minimum peptide chain length. the apparent molecular mass of the proteinase was 27 kda when determined by sds-page and 14.1 kda when measured by gel filtration. the isoelectric point of the proteinase was 9.0. polyclo ... | 1993 | 8360629 |
| cloning and expression of the gene encoding a novel proteinase from tritirachium album limber. | we have isolated the cdna and the genomic clones encoding a novel serine proteinase, named proteinase t, from the fungus tritirachium album limber. the coding region of the gene is interrupted by two introns. the amino acid sequence of proteinase t as deduced from the nucleotide sequence is about 56% identical to that of proteinase k. four cysteines are present in the mature proteinase, probably in the form of disulfide bonds. we have also purified the native proteinase from tritirachium album l ... | 1996 | 8796313 |
| interaction of proteases with legume seed inhibitors. molecular features. | after having found that raw black beans (phaseolus vulgaris) were toxic, while the cooked ones constitute the basic diet of the underdeveloped peoples of the world, in the sixties, our research directed by dr. jaffé, concentrated mainly around the detection and identification of the heat labile toxic factors in legume seeds. a micromethod for the detection of protease inhibitors (pi) in individual seeds was developed, for the purpose of establishing that the multiple trypsin inhibitors (ti) foun ... | 1996 | 9137634 |
| the crystallographic structure of the subtilisin protease from penicillium cyclopium. | the major extracellular protease from the fungus pencillium cyclopium was crystallized in the presence of p-phenylmethanesulfonyl fluoride (pmsf) and investigated by x-ray diffraction analysis. it was subsequently cloned and the amino acid sequence deduced from its cdna. although the sequence is only 49% identical to that of proteinase k of tritirachium album, the three-dimensional structures of the two proteases are virtually identical. the model for p. cyclopium protease was refined by simulat ... | 1997 | 9184139 |
| antigenic relationships among pathogenic beauveria bassiana with engyodontium album (= b. alba) and non-pathogenic species of the genus beauveria. | exoantigenic extracts of 15 isolates belonging to hyalohyphomycosis-causing beauveria bassiana (1), and engyodontium album (1), as well as other species of the genus beauveria (one isolate each of b. brogniartii, b. densa, b. stephanoderis, b. velata, b. vermiconia and six isolates of unknown beauveria species) were studied. aqueous-merthiolated extracts derived from 10-day-old sabouraud's dextrose agar slant cultures (25 degrees c) were concentrated (25x), and reacted against rabbit anti-b. bas ... | 1997 | 9404019 |
| molecular recognition of cobalt(iii)-ligated peptides by serine proteases: the role of electrostatic effects. | a series of peptides with a positively charged cobalt(iii)-complex group attached to the carboxylate terminal was synthesized. the behavior of these metallopeptides as acceptor nucleophiles in acyl transfer reactions catalyzed by the three serine proteases bovine pancreatic alpha-chymotrypsin, porcine pancreatic trypsin, and proteinase k from tritirachium album was examined. the efficiency of the substrates was assessed by kinetic measurement of the partition between aminolysis and hydrolysis. t ... | 1998 | 9832305 |
| [fungal flora in chicken stalls and its etiopathogenic importance for humans and animals]. | in order to find out the mycoflora prevailing in chicken pens, and to appreciate the health hazards for employees (incl. veterinarians) and animals, twenty litter samples and 6 sedimented dust trials were analysed mycologically. the following results were found: bedding and dust samples all contained between 3.57 and 1.30 x 10(7) c.f.u./g dw. the commonest fungus is a. fumigatus with 3.41 x 10(4) - 1.30 x 10(7) c.f.u./g dw in bedding and 2.70 x 10(5) - 3.30 x 10(6) c.f.u./g dw in sedimented dust ... | 1999 | 10209909 |
| biodegradation of fluoranthene by soil fungi. | a selection of 39 strains of micromycetes known as good degraders of polychlorinated aromatic compounds, mostly isolated from soil and belonging to various taxonomic groups, have been investigated for fluoranthene degradation. toxicity assays, first evaluated on solid medium mea, have not shown any toxicity of fluoranthene (1-100 mg.l-1) towards fungi. whereas, consumption assays on a solid synthetic medium showed a toxicity at 100 mg.l-1. the degradation of fluoranthene (10 mg.l-1) was then inv ... | 1999 | 10230046 |
| comparison of the fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms isolated from a temperate agricultural soil. | strains of hydrocarbon-degrading microorganisms (bacteria and fungi) were isolated from an agricultural soil in france. in a field, a portion was treated with oily cuttings resulting from the drilling of an onshore well. the cuttings which were spread at the rate of 600 g hc m-2 contained 10% of fuel oil hydrocarbons (hc). another part of the field was left untreated. three months after hc spreading, hc adapted bacteria and fungi were isolated at different soil depths in the two plots and identi ... | 1999 | 10231986 |
| synthesis and enzymatic degradation of optically active depsipeptide copolymers. | this paper describes the synthesis and biodegradation of copolymers of cyclic depsipeptide with epsilon-caprolactone (cl) or lactide (la). optically active cyclic depsipeptides, 3,6-dimethyl-2,5-morpholinediones (dmos), were prepared by the reaction of an amino acid (d-, l-, or dl-alanine) with a hydroxy acid derivative (dl-2-bromopropionyl bromide). these isomers are abbreviated as d-dmo, l-dmo and dl-dmo respectively, according to the names of alanine isomers. then, we have prepared the copoly ... | 1999 | 10385224 |
| structure of a serine protease proteinase k from tritirachium album limber at 0.98 a resolution. | x-ray diffraction data at atomic resolution to 0.98 a with 136 380 observed unique reflections were collected using a high quality proteinase k crystals grown under microgravity conditions and cryocooled. the structure has been refined anisotropically with refmac and shelx-97 with r-factors of 11.4 and 12.8%, and r(free)-factors of 12.4 and 13.5%, respectively. the refined model coordinates have an overall rms shifts of 0.23 a relative to the same structure determined at room temperature at 1.5 ... | 2001 | 11258922 |
| mercury induced modifications in the stereochemistry of the active site through cys-73 in a serine protease--crystal structure of the complex of a partially modified proteinase k with mercury at 1.8 a resolution. | proteinese k (pk) isolated from tritirachium album limber was crystallized with hgcl2 in excess, under microgravity conditions. the intensity data were collected at 4 degrees c to 1.8 a resolution and the final r-factor after refinement for all the reflections was 0.164. mercury has been found at two sites with partial occupancies (0.4 and 0.6) which are at distances of 2.48 a and 2.58 a respectively from cys-73 sgamma. the cys-73 in the enzyme structure is located close to the active site resid ... | 2001 | 11886076 |
| microorganisms cultured from stratospheric air samples obtained at 41 km. | samples of air removed from the stratosphere, at an altitude of 41 km, were previously found to contain viable, but non-cultureable bacteria (cocci and rods). here, we describe experiments aimed at growing these, together with any other organisms, present in these samples. two bacteria (bacillus simplex and staphylococcus pasteuri) and a single fungus, engyodontium album (limber) de hoog were isolated from the samples. although the possibility of contamination can never be ruled out when space-d ... | 2003 | 12583913 |
| expression and purification of antimicrobial peptide adenoregulin with c-amidated terminus in escherichia coli. | adenoregulin is a 33 amino acid antimicrobial peptide isolated from the skin of the arboreal frog phyllomedusa bicolor. natural adenoregulin is synthesized with an amidated valine residue at c-terminus and shows lethal effects against filamentous fungi, as well as a broad spectrum of pathogenic microorganisms. a synthetic gene for adenoregulin (adr) with an additional amino acid glutamine at c-terminus was cloned into pet32a vector to allow expression of adr as a trx fusion protein in escherichi ... | 2005 | 15766883 |
| a preliminary study of the occurrence of actidione-resistant fungi in sediments of catalonian river mouths (spain). i. keratinolytic fungi and related onygenales. | sediments from eight river mouths along the catalonian coast (spain) were surveyed for keratinolytic fungi and related onygenales. the actidione plating technique was employed. of 532 actidione-treated sediment samples, 268 (50.3%) were positive for the fungi. altogether, 384 fungal strains from 35 species were isolated from the samples. narasimhella marginospora, aphanoascus fulvescens, neoarachnotheca keratinophila with its anamorph myriodontium keratinophilum, narasimhella hyalinospora, beauv ... | 1998 | 16284860 |
| characterization of a recombinantly expressed proteinase k-like enzyme from a psychrotrophic serratia sp. | the gene encoding a peptidase that belongs to the proteinase k family of serine peptidases has been identified from a psychrotrophic serratia sp., and cloned and expressed in escherichia coli. the gene has 1890 base pairs and encodes a precursor protein of 629 amino acids with a theoretical molecular mass of 65.5 kda. sequence analysis suggests that the peptidase consists of a prepro region, a catalytic domain and two c-terminal domains. the enzyme is recombinantly expressed as an active approxi ... | 2006 | 16367747 |
| the 1.8 a crystal structure of a proteinase k-like enzyme from a psychrotroph serratia species. | proteins from organisms living in extreme conditions are of particular interest because of their potential for being templates for redesign of enzymes both in biotechnological and other industries. the crystal structure of a proteinase k-like enzyme from a psychrotroph serratia species has been solved to 1.8 a. the structure has been compared with the structures of proteinase k from tritirachium album limber and vibrio sp. pa44 in order to reveal structural explanations for differences in biophy ... | 2006 | 16367748 |
| biodegradability and biodegradation of poly(lactide). | poly(lactide) (pla) has been developed and made commercially available in recent years. one of the major tasks to be taken before the widespread application of pla is the fundamental understanding of its biodegradation mechanisms. this paper provides a short overview on the biodegradability and biodegradation of pla. emphasis is focused mainly on microbial and enzymatic degradation. most of the pla-degrading microorganisms phylogenetically belong to the family of pseudonocardiaceae and related g ... | 2006 | 16823551 |
| cloning a cuticle-degrading serine protease gene with biologic control function from beauveria brongniartii and its expression in escherichia coli. | beauveria brongniartii extracellular subtilisin-like serine protease (pr1) is one of the most virulent factors by virtue of its activity against insect cuticles. the pr1 cdna was cloned using the switching mechanism at the 5' end of the rna transcript and rapid amplification of cdna ends. the 1732-bp fragment of genomic dna containing the predicted open-reading frame of the pr1 gene was cloned by polymerase chain reaction and sequenced. the pr1 cdna is 1550 bp and contains an 1140-bp orf. the de ... | 2006 | 16832726 |
| variability in tolerance to uv-b radiation among beauveria spp. isolates. | solar radiation, particularly the uv-b component, negatively affects survival of entomopathogenic fungi in the field. in an effort to identify beauveria spp. isolates with promise for use in biological control settings with high insolation, we examined 53 beauveria bassiana isolates, 7 isolates of 4 other beauveria spp. and engyodontium albus (=beauveria alba). the origins of these fungi varied widely as to host/substrate and country, but approximately 30% of these isolates were b. bassiana from ... | 2007 | 17610892 |
| cold activity of beauveria and metarhizium, and thermotolerance of beauveria. | heat and cold are environmental abiotic factors that restrict the use of entomopathogenic fungi as agents for biological control of insects. the thermotolerance and cold activity of 60 entomopathogenic fungal isolates, including five species of beauveria and one isolate of engyodontium albus (=beauveria alba) were examined as to tolerance of temperatures that might be encountered during field use. in addition, cold activity of eight metarhizium spp. isolates was evaluated. the isolates were from ... | 2008 | 18096184 |
| the novel inhibitors of serine proteases. | thirty optically active nonprotein alpha-amino acids and peptides based thereon have been screened for their ability to interact with bovine trypsin and proteinase k from tritirachium album limber, which belong to the group of serine proteases. both structure-based drug design approach and determination of enzyme activity have been used to identify low molecular weight inhibitors of trypsin and proteinase k. compounds have been selected that according to the docking analysis were able to interac ... | 2009 | 19326044 |
| effect of alkyl alcohols on partially unfolded state of proteinase k: differential stability of alpha-helix and beta-sheet rich regions of the enzyme. | proteinase k (e.c. 3.4.21.64), a serine proteinase from fungus tritirachium album, has been used as a model system to investigate the conformational changes induced by monohydric alcohols at low ph. proteinase k belongs to alpha/beta class of proteins and maintains structural integrity in the range of ph 7.0-3.0. enzyme acquires partially unfolded conformation (u(p)) at ph 2.5 with lower activity, partial loss of tertiary structure and exposure of some hydrophobic patches. proteinase k in stress ... | 2009 | 19403104 |
| biodegradation of physicochemically treated polycarbonate by fungi. | two fungal strains isolated from soil and a commercial white-rot fungus, phanerochaete chrysosporium ncim 1170 (sf2), were tested for biodegradation of untreated, uv-, and thermal-treated bisphenol a polycarbonate (pc). the isolated strains based on 18s rdna analysis were characterized as engyodontium album mtp091 (sf1) and pencillium spp. mtp093 (sf3). about 5.4% weight loss and 40% reduction in m(n) were observed for uv-treated polycarbonate in one year with sf2 strain. an increase in surface ... | 2010 | 19961236 |
| the crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes. | cuticle-degrading proteases are involved in the breakdown of cuticle/eggshells of nematodes or insects, a hard physical barrier against fungal infections. understanding the 3-dimensional structures of these proteins can provide crucial information for improving the effectiveness of these fungi in biocontrol applications, e.g., by targeted protein engineering. however, the structures of these proteases remain unknown. here, we report the structures of two cuticle-degrading proteases from two spec ... | 2010 | 20007510 |
| a case of bovine valve endocarditis caused by engyodontium album. | we report the first case of engyodontium album bioprosthetic valve endocarditis in a 44-year-old male with a history of juvenile rheumatoid arthritis. there is only one other report of engyodontium album as a human pathogen. the present case supports the increased incidence of fungal endocarditis especially in patients receiving immunotherapy. | 2010 | 21108570 |
| characterization of an extracellular alkaline serine protease from marine engyodontium album btmfs10. | an alkaline protease from marine engyodontium album was characterized for its physicochemical properties towards evaluation of its suitability for potential industrial applications. molecular mass of the enzyme by matrix-assisted laser desorption ionization-mass spectrometry (maldi-ms) analysis was calculated as 28.6 kda. isoelectric focusing yielded pi of 3-4. enzyme inhibition by phenylmethylsulfonyl fluoride (pmsf) and aprotinin confirmed the serine protease nature of the enzyme. k (m), v (ma ... | 2010 | 21110062 |
| An intensive search for promising fungal biological control agents of ticks, particularly Rhipicephalus microplus. | Entomopathogenic fungi have been investigated worldwide as promising biological control agents of the cattle tick Rhipicephalus microplus. The current study evaluates the virulence of several fungal isolates to R. microplus larva in the laboratory as part of an effort to identify isolates with promise for effective biocontrol of R. microplus in the field. Sixty fungal isolates, encompassing 5 Beauveria spp. and 1 Engyodontium albus (=Beauveria alba), were included in this study. In addition to b ... | 2011 | 21705145 |
| isolation of keratinophilic fungi from soil in isfahan province, iran. | to evaluate the epidemiology of keratophilic fungi in isfahan province, iran. | 2011 | 23177808 |