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isolation and characterization of a succinylated polysaccharide from the cell wall of micrococcus agilis.a polysaccharide consisting of rhamnose, galactose, glucosamine and ester-linked succinic acid was extracted from the isolated cell walls of micrococcus agilis by the hot water-phenol and 5% trichloroacetic acid (tca) extraction methods. the hot water-phenol extractable polysaccharide accounted for 30% of the weight of the wall, with 23% by the tca method. phosphorus contents were less than 0.01% of the polysaccharide. succinyl residues released by alkali treatment (0.1 n naoh, 30 min, 37 degree ...19853831716
aminoacylase from micrococcus agilis.intracellular aminoacylase from micrococcus agilis ccm 2131 was purified 430-fold with a 23% yield. the purified enzyme was homogeneous on polyacrylamide-gel electrophoresis and it smolecular weight was 58000. the enzyme hydrolysed stereospecifically a number of acylated l-amino acids. its activity towards n-acetyl-l-phenylglycine was strongly inhibited by 1,10-phenanthroline, n-bromosuccinimide and mercaptoethanol, and was inhibited competitively by glycylglycine.19807435078
morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces.in an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16s rdna sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. all except one strain, which was found to be a bacillus, were members of the order actinomycetales. the majority of the strains either were closely related to geodermatophilus obscurus, which was al ...19968661940
reclassification of micrococcus agilis (ali-cohen 1889) to the genus arthrobacter as arthrobacter agilis comb. nov. and emendation of the genus arthrobacter.phylogenetic evidence derived from a 16s ribosomal dna analysis indicated that the type strain of micrococcus agilis, dsm 20550 (= atcc 966 = ccm 2390), is less closely related to the type species of the genus micrococcus, micrococcus luteus, than to the type species of the genus arthrobacter, arthrobacter globiformis, and related arthrobacter species. the phylogenetic position of m. agilis is supported by the presence of peptidoglycan variation a3 alpha and by the presence of mk-9(h2) as the ma ...19957547308
continuous production of indole-3-acetic acid by immobilized cells of arthrobacter agilis.indole acetic acid (iaa) is a plant growth-promoting hormone used in agriculture; therefore, its continuous production is of paramount importance. iaa-producing eight bacteria were isolated from the rhizosphere of verbascum vulcanicum. among them, arthrobacter agilis a17 gave maximum iaa production (75 mg/l) and this strain was used to immobilization studies. the a. agilis a17 cells were immobilized in calcium alginate for the production of iaa. optimization of process parameters for iaa product ...201728401461
isolation of endophytic plant growth-promoting bacteria associated with the halophyte salicornia europaea and evaluation of their promoting activity under salt stress.several reports have highlighted that many plant growth-promoting endophytic bacteria (pgpe) can assist their host plants in coping with various biotic and abiotic stresses. however, information about the pgpe colonizing in the halophytes is still scarce. this study was designed to isolate and characterize pgpe from salt-accumulating halophyte salicornia europaea grown under extreme salinity and to evaluate in vitro the bacterial mechanisms related to plant growth promotion. a total of 105 isola ...201627447799
first high quality draft genome sequence of a plant growth promoting and cold active enzyme producing psychrotrophic arthrobacter agilis strain l77.arthrobacter agilis strain l77, is a plant growth promoting and cold active hydrolytic enzymes producing psychrotrophic bacterium, isolated from pangong lake, a subglacial lake in north western himalayas, india. genome analysis revealed metabolic versatility with genes involved in metabolism and cold shock adaptation, utilization and biosynthesis of diverse structural and storage polysaccharides such as plant based carbon polymers. the genome of arthrobacter agilis strain l77 consists of 3,608,4 ...201627570579
cloning and characterization of cold-adapted α-amylase from antarctic arthrobacter agilis.in this study, the gene encoding an α-amylase from a psychrophilic arthrobacter agilis pamc 27388 strain was cloned into a pet-28a(+) vector and heterologously expressed in escherichia coli bl21(de3). the recombinant α-amylase with a molecular mass of about 80 kda was purified by using ni(2+)-nta affinity chromatography. this recombinant α-amylase exhibited optimal activity at ph 3.0 and 30 °c and was highly stable at varying temperatures (30-60 °c) and within the ph range of 4.0-8.0. furthermor ...201727714640
[the plant growth-promoting rhizobacterium arthrobacter agilis umcv2 endophytically colonizes medicago truncatula].arthrobacter agilis umcv2 is a rhizosphere bacterium that promotes legume growth by solubilization of iron, which is supplied to the plant. a second growth promotion mechanism produces volatile compounds that stimulate iron uptake activities. additionally, a. agilis umcv2 is capable of inhibiting the growth of phytopathogens. a combination of quantitative polymerase chain reaction and fluorescence in situ hybridization techniques were used here to detect and quantify the presence of the bacteriu ...201627916328
the rhizobacterium arthrobacter agilis produces dimethylhexadecylamine, a compound that inhibits growth of phytopathogenic fungi in vitro.plant diseases caused by fungal pathogens such as botrytis cinerea and the oomycete phytophthora cinnamomi affect agricultural production worldwide. control of these pests can be done by the use of fungicides such as captan, which may have deleterious effects on human health. this study demonstrates that the rhizobacterium arthrobacter agilis umcv2 produces volatile organic compounds that inhibit the growth of b. cinerea in vitro. a single compound from the volatile blends, namely dimethylhexade ...201323674267
characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients.over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ...200818335166
arthrobacter pityocampae sp. nov., isolated from thaumetopoea pityocampa (lep., thaumetopoeidae).a bacterium (strain tp2(t)) was isolated from a caterpillar of the pine processionary moth, thaumetopoea pityocampa (den. & schiff.) (lepidoptera: thaumetopoeidae), a destructive pine forest pest. the bacterium is a gram-stain-positive, red-pigmented coccus, oxidase-negative, nitrate-reducing, non-motile and non-spore-forming. strain tp2(t) was subjected to a taxonomic study using polyphasic approach that included morphological and biochemical characterizations, 16s rrna gene sequence analysis, ...201425013228
secondary metabolites: applications on cultural heritage.biological sciences and related bio-technology play a very important role in research projects concerning protection and preservation of cultural heritage for future generations. in this work secondary metabolites of burkholderia gladioli pv. agaricicola (bga) icmp 11096 strain and crude extract of glycoalkaloids from solanaceae plants, were tested against a panel of microorganisms isolated from calcarenite stones of two historical bridges located in potenza and in campomaggiore (southern italy) ...201325145230
[bacterium arthrobacter agilis umcv2 and diverse amines inhibit in vitro growth of wood-decay fungi].the kingdom fungi is represented by a large number of organisms, including pathogens that deteriorate the main structural components of wood, such as cellulose, hemicellulose and lignin. the aim of our work was to characterize the antifungal activity in arthrobacter agilis umcv2 and diverse amines against wood-decaying fungi. four fungal organisms (designated as umtm) were isolated from decaying wood samples obtained from a forest in cuanajo-michoacán, méxico. two of them showed a clear enzymati ...201526350556
arthrobacter echini sp. nov., isolated from the gut of a purple sea urchin, heliocidaris crassispina.a novel strain, designated am23t, was isolated from the gut of a purple sea urchin heliocidaris crassispina collected from the coastal waters of the korean island dokdo. 16s rrna gene sequence analysis showed that strain am23t belonged to the genus arthrobacter in the family micrococcaceae and shared highest sequence similarity with arthrobacter agilis dsm 20550t (98.77%). strain am23t was catalase-positive, oxidase-negative and grew optimally at 20 °c, in the presence of 1% (w/v) nacl and at ph ...201626868361
bacterial diversity from benthic mats of antarctic lakes as a source of new bioactive metabolites.during the micromat project, the bacterial diversity of microbial mats growing in the benthic environment of antarctic lakes was accessed for the discovery of novel antibiotics. in all, 723 antarctic heterotrophic bacteria belonging to novel and/or endemic taxa in the +¦-, +¦- and +¦-subclasses of the proteobacteria, the bacteroidetes branch, and of the high and low percentage g+c gram-positives, were isolated, cultivated in different media and at different temperatures, and then screened for th ...200921798170
biochemical and phylogenetic analyses of psychrophilic isolates belonging to the arthrobacter subgroup and description of arthrobacter psychrolactophilus, sp. nov.during our work on psychrophilic microorganisms we obtained a large collection of new isolates. in order to identify six of these, we examined their growth properties, cell wall compositions, and their 16s rrna gene sequences. the results showed that all of the isolates are gram-positive, aerobic, contain lysine in their cell walls, and belong to the high mol% g+c arthrobacter subgroup. phylogenetic analysis of the 16s rrna genes grouped five isolates obtained from a small geographical region in ...199910369891
carotenoid accumulation in the psychrotrophic bacterium arthrobacter agilis in response to thermal and salt stress.a psychrotrophic strain of arthrobacter agilis, isolated from antarctic sea ice, grows from 5 degrees c to 40 degrees c and in culture media containing 0-10% (w/v) nacl. maximum growth rate occurred at 30-35 degrees c with a drastic decline as the cultivation temperatures diverged. adaptation to extremes of low temperature may be partially attributed to the production of the c-50 carotenoid bacterioruberin, and its glycosylated derivatives. lowering of the cultivation temperature resulted in a c ...200111601625
arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in mcmurdo dry valley, antarctica.cms 19yt, a psychrophilic bacterium, was isolated from a cyanobacterial mat sample from a pond in antarctica and was characterized taxonomically. the bacterium was aerobic, gram-positive, non-spore-forming, non-motile, exhibited a rod-coccus growth cycle and produced a yellow pigment that was insoluble in water but soluble in methanol. no growth factors were required and it was able to grow between 5 and 30 degrees c, between ph 6 and ph 9 and tolerated up to 11.5% nacl. the cell wall peptidogly ...200010939663
chiral inversion of drug: role of intestinal bacteria in the stereoselective sulphoxide reduction of flosequinan.chiral inversion at a sulphoxide position of flosequinan enantiomers [(+/-)-7-fluoro-1-methyl-3-methylsulphinyl-4-quinolone] occurred in conventional rats but not in either germ-free rats or rats treated with antibiotics after an oral administration of each enantiomer. thus, it was postulated that the chiral inversion occurred by mechanisms mediated by intestinal bacteria. the intestinal content isolated from conventional rats reduced r(+)- and s(-)-flosequinan to produce the sulphide, while int ...19948053920
bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of lonar, india.phenol is an industrially important compound which has a wide range of applications. being highly soluble in water, it appears as the major pollutant in waste waters arising from both phenol manufacturing and from industrial units that utilise phenol. because of its toxicity, bioremediation of phenol is necessary. since some of the phenol-bearing industrial waste waters are alkaline in nature, use of alkaliphilic bacteria for bioremediation of phenol was investigated. alkaliphilic bacteria were ...199821182701
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