| [interferon induced in the hamster by subunits of "nocardia": essay of protection against rabies (author's transl)]. | lipid-free cells of nocardia opaca and a cell extract (nwsm) are interferon inducers in syrian hamsters: peak interferon appears 2 h after injection as compared to 8 h in the case of interferon induced by inactivated ndv virus. the interferon induced by nocardia product is inactivated by a treatment for 30 min at 56 degrees c or 24 h at ph 2: ndv-induced interferon is more stable under these conditions. hamsters inoculated with nwsm before and after infection with rabies virus are partially prot ... | 1979 | 43690 |
| [steroid-transforming enzymes from microorganisms. ix. affinity chromatographic preparation and studies of the apoenzyme from a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca]. | from the flavoenzyme, 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase of nocardia opaca, prosthetic group and apoenzyme were separated quantitatively by means of affinity chromatography in the presence of 2 m (nh4)2 at ph 3.0. subsequently the apoenzyme was eluted from affinity matrix by 0.01 m phosphate buffer, ph 8.0, whereas under these conditions the intact enzyme could not be eluted. the whole enzyme activity applied could be restored by incubation of the eluted apoenzyme with fad. the bi ... | 1979 | 44397 |
| [steroid-transforming enzymes from microorganisms. xi. reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from nocardia opaca]. | highly purified preparations of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca have been investigated in both types of reactions: 1.2-dehydrogenation of the 4-en-3-oxo-derivative and 1.2-hydrogenation of the 1.4-dien-3-oxo-derivative. it was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, sds-electrophoresis, and isoelectric focusing techniques. the pure enzyme preparation is discus ... | 1979 | 44773 |
| [isolation of an autotrophic variant of nocardia opaca]. | a hydrogen-oxidizing autotrophic actinomycete, strain z-766, was isolated from the neuston of a marsh near moscow. the organism was identified as nocardia opaca z-766 according to its morphologo-physiological properties. the spectrum of organic compounds used by the strain, its cultural characteristics and growth are similar to those of the monotypical autotrophic microorganism n. opaca 1b described earlier. repeated isolation of the autotrophic variant of n. opaca z-766 suggests that the abilit ... | 1979 | 155775 |
| [steroid-transforming enzymes from microorganisms. xii. induction characteristics of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase in nocardia opaca]. | 17 alpha-methyltestosterone and the corresponding 1(2)-dehydrocompound (dianabol) are efficient inducers of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca. after a lag period of 4 hours the enzyme activity increases rapidly. during the induction the steroids are completely metabolized causing a drastical drop of specific enzyme activity. using a fixed induction time the optimal steroid concentration and the temperature characteristic were found out. the influence of th ... | 1979 | 547499 |
| hydrogen metabolism in aerobic hydrogen-oxidizing bacteria. | a survey on organisms able to use molecular hydrogen as electron donor in the energy-yielding process is presented. in the group of the aerobic hydrogen-oxidizing bacteria so far two types of hydrogenases have been encountered, a nad-reducing, soluble enzyme (h2 : nad oxidoreductase) and a membrane-bound enzyme unable to reduce pyridine nucleotides. with respect to the distribution of both types of hydrogenases three groups of hydrogen-oxidizing bacteria can be diffentiated containing (i) both t ... | 1978 | 667183 |
| [steroid metabolizing enzymes from microorganisms. vii. the nature of the biospecific binding to an affinity matrix-affinity chromatography of 4-en-3-oxo-steroid: (acceptor)-1-en-oxidoreductase from nocardia opaca]. | | 1978 | 692123 |
| [steroid converting enzymes from microorganisms. viii. effect of the organic solvent specific hydrophobicity of the binding on the affinity-matrix- affinity chromatography of a 4-en-3-oxo-steroid: (acceptor)-1-en-oxidoreductase from nocardia opaca]. | | 1978 | 692134 |
| [steroid transforming enzymes from microorganisms. v. purification of a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca and characterization of the prosthetic group]. | | 1978 | 695702 |
| [steroid conjugating enzyme from microorganisms. vi. purification of 4-en-o-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca by affinity chromatography]. | | 1977 | 916673 |
| [breakdown of steroids. xv. formation of 9, 10-seco-compounds from 4-chlorosubstituted steroids in nocardia opaca]. | | 1976 | 933521 |
| [steroid-transforming enzymes from microorganisms. ii. purification of a 3-oxosteroid: (2,6-dichlorophenolindophenol) delta 1-oxidoreductase from nocardia opaca by means of affinity chromatography]. | | 1976 | 983139 |
| [degradation of steroids. xiv. synthesis of [1-14c]-succinic acid and [5-14c]-levulinic acid from 7a-methyl-5,6,7,7a-tetrahydroindan-[5-14c]-1,5-dion-4-(3-propionic acid-[1-14c]) by nocardia opaca]. | | 1975 | 1052854 |
| a human t-independent b lymphocyte mitogen extracted from nocardia opaca. | nocardia water soluble mitogen (nwsm), a mitogen specific for mouse and rabbit b cells, activates blood, spleen, and tonsil lymphocytes from all the humans tested. evidence is presented that it acts selectively on human b lymphocytes. b-enriched cells were recovered from anti-ig-coated sephadex columns or after elimination of e rosette-forming cells: they were able to incorporate tritiated thymidine when they were cultivated in the presence of nwsm whereas they were not activated by pwm. convers ... | 1976 | 1087313 |
| isolation of mitogenic and adjuvant active fractions from various species of nocardiae. | delipidated lysozyme digests of nocardia opaca, n. corallina, and n. rubra have been fractionated by sephadex filtration. the mitogenic and adjuvant activities of the fractions thus obtained have been investigated. all fractions are mitogenic except the last fraction of n.rubra, but the n. opaca products induce a stronger stimulation of mouse spleen lymphocytes than the corresponding fractions of the two other species. the activity of the first sephadex fractions of each strain has been compared ... | 1975 | 1089598 |
| free mycolic acids as criteria in the classification of nocardia and the 'rhodochrous' complex. | the methyl esters of free mycolic acids from representative strains of nocardia asteroides, n. brasiliensis, n. caviae and the 'rhodochrous' complex were subjected to detailed mass spectral analysis. the anhydromycolic esters of the nocardia strains consisted of homologous series containing from zero to three double bonds, with the main components of the parent mycolic acids centred on c52 to c54 (range c46 to c58). the anhydromycolates from one rhodochrous strain, nocardia opaca, had a molecula ... | 1976 | 1107481 |
| [activity of microorganisms decomposing cholesterol]. | the rates of cholesterol decomposition was compared among cell suspensions of different microorganisms. fifty seven cultures were studied: 2 strains of actinomycetes, 23 strains of proactinomycetes, 22 strains of mycobacteria, and 10 strains of bacteria. during four hours of incubation, 11 strains virtually did not decompose cholesterol at all, 10 strains decomposed it by up to 20 per cent, 21 strains by 20 to 70 per cent, and 15 strains by 70 to 100 per cent. the highest activity was displayed ... | 1975 | 1226133 |
| presence and subcellular localization of two distinct mitogenic fractions in the cells of nocardia rubra and nocardia opaca: preparation of soluble mitogenic peptidoglycan fractions. | fractionation of cells of nocardia rubra and nocardia opaca led to the separation of the cell wall and a "cytoplasmic" fraction. both fractions were mitogenic for the splenocytes of akr and nude mice and of rabbits. the peptidoglycan was the active part of the cell wall fraction. the products solubilized by the action of streptomyces albus g peptidases on the peptidoglycan of n. rubra were mitogenic, but the products solubilized by lysoyme were not. tentative structures are proposed for these fr ... | 1976 | 1278999 |
| phagocytic response modifying reactivity of enzymatic cell wall digests of nocardia opaca. | aqueous extracts (enocw) and enzymatic digests of purified nocardia opaca cell wall fragments, virtually free of muramyl peptides, were monitored for their phagocytic response modifying reactivity on polymorphonuclear leucocytes, separated or unseparated in whole human blood. in the presence of enocw a 74% increased production of superoxide during the respiratory burst of tpa-activated polymorphonuclear leukocytes was observed, as compared to the unprimed control. delipidation of this preparatio ... | 1990 | 2177449 |
| hydrogen autotrophy of nocardia opaca strains is encoded by linear megaplasmids. | several linear megaplasmids were detected in the facultatively lithoautotrophic gram-positive bacterium nocardia opaca. the wild-type strain mr11 contains, in addition to the cccdna plasmids phg31-a and phg31-b, the linear plasmids phg201 (270 kb), phg202 (400 kb) and phg203 (420 kb). the wild-type strain mr22 contains, in addition to the cccdna plasmid phg33, the linear plasmids phg204 (180 kb), phg205 (280 kb) and phg206 (510 kb). after preparation of dna from cells embedded in agarose, the li ... | 1990 | 2200846 |
| intrinsic b lymphocyte defect in untreated patients with hodgkin's disease. | in vivo and in vitro humoral and cell-mediated immunological defects have been described in untreated patients with hodgkin's disease (hd). the cellular basis of the recently described in vitro reduction of mitogen-induced immunoglobulin synthesis has not been elucidated so far. in this study, we attempted to dissect t and b lymphocyte function in untreated hd patients. mitogen-induced in vitro immunoglobulin synthesis was assessed in the presence of pokeweed mitogen, the mitogenic anti-cd3 mono ... | 1990 | 2261600 |
| enhancement of natural killer cell activity by nocardia opaca fractions. | three molecules derived from nocardia opaca bacteria, ndcm, nwsmp, and pg, have been shown to express immunomodulating properties. the present study was aimed at assessing the effects of these derivatives on natural killer (nk) activity. two experimental protocols were adopted, consisting of incubating whole or percoll fractionated nk cells in vitro with those substances, and the other in which the derivatives were administered in vivo to mice and the activity assessed later. incubation of splee ... | 1989 | 2466328 |
| comparison of the nh2-terminal amino acid sequences of the four non-identical subunits of the nad-linked hydrogenases from nocardia opaca 1b and alcaligenes eutrophus h16. | the cytoplasmic, nad-linked hydrogenase of the gram-positive hydrogen-oxidizing bacterium nocardia opaca 1b was compared with the analogous enzyme isolated from the gram-negative bacterium alcaligenes eutrophus h16. the hydrogenase of n. opaca 1b was purified by a new procedure applying chromatography on phenyl-sepharose and deae-sephacel with two columns in series. a homogeneous enzyme preparation with a specific activity of 74 mumol h2 oxidized.min-1.mg protein-1 and a yield of 32% was isolate ... | 1989 | 2496982 |
| antimetastatic effect of immunomodulators from nocardia opaca in mice and rats activation of peritoneal macrophages by these fractions. | nocardia delipidated cell mitogen (ndcm), a particulate fraction prepared from nocardia opaca, injected i.p. in an oil/water emulsion to f6 rhabdomyosarcoma-bearing rats, inhibited the development of pulmonary metastases; 6 out of 10 rats were protected. repeated i.p. administration of emulsified ndcm and of two other compounds, a nocardia water soluble mitogen (nwsm a hydrosoluble fraction) and purified cell walls (cw, an insoluble macromolecular fraction) in lewis lung carcinoma (llc)-bearing ... | 1987 | 3117366 |
| suppression of polyclonal b cell activation by igg-binding factors. requirement for t cells. | igg-binding factors (igg-bf) prepared from cell-free supernatant of human peripheral blood mononuclear cells interfere with the polyclonal activation of peripheral b cells by decreasing the numbers of igg-containing cells and ig plaque-forming cells. using nocardia opaca delipidated cell mitogen (ndcm), a t helper cell-independent polyclonal b cell activator, it was found that the suppressive effect of igg-bf was no longer demonstrable after removal of t cells. in pokeweed mitogen-stimulated cul ... | 1985 | 3155689 |
| a novel lymphokine derived from human igg fc receptor-bearing b cells: its suppressive effect on activated t and b lymphocytes including tumour cells in vitro. | peripheral blood fcr gamma-bearing human b cells, but neither t cells nor adherent cells, produce an immunoregulatory lymphokine after receiving the stimulation of fcr gamma by immune complexes--antibody-sensitized erythrocytes (ea). this factor suppresses polyclonal immunoglobulin (ig) production of b cells to pokeweed mitogen (pwm) and nocardia opaca delipidated cell mitogen (ndcm), indicating that not only b cells, but also t cells are targets for this factor. it also inhibits the proliferati ... | 1985 | 3160654 |
| stimulation of sea star asterias rubens axial organ b-like cells by nocardia-delipidated cell mitogen and derived fractions. | the axial organ (ao-cells) of the starfish asterias rubens is a primitive immune organ. the total population was fractionated into two populations: adherent (b-like) and non-adherent (t-like) to nylon wool. nocardia-delipidated cell mitogen (ndcm), nocardia water soluble mitogen (nwsm), nwsm-pellet (nwsmp) and purified cell walls (cw) from nocardia opaca induce the proliferation of b-like cells from the axial organ. the nocardia immunomodulators studied are inactive on the total and t-like popul ... | 1988 | 3203818 |
| suppression of human iga-b-cell maturation by iga-binding factors requires t cells. | iga-binding factors (iga-bfs) prepared from human peripheral blood mononuclear cells selectively decrease the generation of cytoplasmic iga-positive cells in peripheral blood mononuclear cell cultures stimulated by pokeweed mitogen or by nocardia opaca delipidated cell mitogen. in n. opaca delipidated cell mitogen stimulated cultures, the suppressive effect of iga-bfs was no longer demonstrable after removal of sheep erythrocyte rosetting t cells or after depletion of cd8+ cells, but it was not ... | 1988 | 3265931 |
| application of chromatographic methods to the analysis of macrophage factors induced by nocardia opaca cell walls. | cell walls from nocardia opaca induce the production of mitogenic factors by mouse peritoneal macrophages in vitro. these factors stimulate thymocytes from c3h/hej mice. supernatants of peritoneal cell culture exhibiting this activity were fractionated by chromatographic procedures such as gel filtration and metal chelate affinity chromatography and the biological activities assayed. these fractionation studies indicate that several biologically active products occur in the supernatant. four fac ... | 1988 | 3403659 |
| no correlation exists between the conjugative transfer of the autotrophic character and that of plasmids in nocardia opaca strains. | a new isolate of nocardia opaca was obtained by enrichment culture for aerobic lithoautotrophic growth on co2 and h2. this strain, mr22, is very similar to n. opaca mr11 (formerly 1b) in functioning as a donor for genetic information determining the ability to grow lithoautotrophically (aut character) in matings with aut- strains of n. opaca or closely related heterotrophic species. the strain contains a plasmid, phg33 of about 110 kb. a mutant was isolated from strain mr22 which was plasmid-fre ... | 1986 | 3463670 |
| nocardia water soluble mitogen (nwsm), a human b-lymphocyte mitogen. | nocardia water soluble mitogen (nwsm), which is a peptidoglycan, was prepared from nocardia opaca. its effect on human b-lymphocyte proliferation, the number of immunoglobulin secreting b-cells, and bone marrow stem cell growth was studied in vitro. nwsm stimulated the proliferation of b-lymphocytes of all ten healthy subjects studied very effectively (p less than 0.0005). no t-cell activation occurred. nwsm did not increase the amount of antibody producing cells. the growth of bone marrow stem ... | 1986 | 3492453 |
| tumor localization and biodistribution of 99mtc-muramylpolypeptide (mpp) in the rat. | muramylpolypeptide, extracted from nocardia opaca bacteria and originally designed for immunotherapy of malignant tumors, proved to localize in malignant tumors, when labeled with 99mtc. in the tumor-bearing rat 99mtc-muramylpolypeptide (mpp) showed a fast turnover and optimum tumor to soft tissue ratios were obtained as early as between 30-60 min after application. the uptake mechanism in malignant tumors remains uncertain. | 1986 | 3714507 |
| distribution of ig classes and igg subclasses among human b cells activated by nocardia opaca-delipidated cell mitogen or by pokeweed mitogen. | the relative proportions of cells synthesizing the three major ig classes or one of the four igg subclasses in cultures stimulated with pokeweed mitogen (pwm) or nocardia-delipidated cell mitogen (ndcm) were investigated. in cultures of human peripheral blood mononuclear cells (pb mnc) stimulated with pwm, the number of igg-containing cells (cc) was higher than the number of igm-cc, and a substantial number of iga-cc was found. conversely, in ndcm-stimulated pb mnc cultures igm-cc outnumbered ig ... | 1985 | 3875142 |
| stimulation of human b lymphocytes by nocardia-delipidated cell mitogen and derived fractions from n. opaca. structure-activity relationship. | nocardia-delipidated cell mitogen (ndcm), a particulate fraction prepared from nocardia opaca, is able to stimulate the proliferation of small resting human b lymphocytes and their differentiation into ig-secreting cells. this fraction contains two active structures: the cell wall peptidoglycan (pg) and a fraction (cy i) derived from the cytoplasmic compartment. treatment of insoluble pg with various bacteriolytic enzymes showed that the minimal structure required for mitogenic activity is more ... | 1985 | 3930601 |
| mitogenic effect of a water-soluble extract of nocardia opaca: a comparative study with some bacterial adjuvants on spleen and peripheral lymphocytes of four mammalian species. | | 1974 | 4207728 |
| studies on a gram-positive hydrogen bacterium, nocardia opaca 1 b. iii. purification, stability and some properties of the soluble hydrogen dehydrogenase. | | 1974 | 4374147 |
| nwsm, a mitogen from nocardia opaca, specific for rabbit bursa equivalent cells. | | 1974 | 4616010 |
| studies on a gram-positive hydrogen bacterium, nocardia opaca strain 1 b. i. description and physiological characterization. | | 1973 | 4684599 |
| studies on a gram-positive hydrogen bacterium, nocardia opaca strain 1 b. ii. enzyme formation and regulation under the influence of hydrogen or fructose as growth substrates. | | 1973 | 4684600 |
| preparation and biological properties of water-soluble adjuvant fractions from delipidated cells of mycobacterium smegmatis and nocardia opaca. | digestion by lysozyme of delipidated cells of mycobacterium smegmatis liberates a water-soluble immunoadjuvant fraction which is chemically very similar to the water-soluble adjuvant (wsa) obtained previously from purified cell walls, but which contains somewhat more non-peptidoglycan amino acids. the yield of peptidoglycan-arabinogalactan complex is about 10 times greater starting from whole cells than from cell walls. the main biological properties of this "neo-wsa" are described: it increases ... | 1973 | 4716544 |
| distribution of the isopropylmalate pathway to leucine among diverse bacteria. | alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ... | 1974 | 4829932 |
| [on the different types of nocardic acids isolated from nocardia opaca]. | | 1966 | 4958541 |
| utilization of anthranilic and nitrobenzoic acids by nocardia opaca and a flavobacterium. | | 1966 | 5912587 |
| content and localization of fmn, fe-s clusters and nickel in the nad-linked hydrogenase of nocardia opaca 1b. | by preparative polyacrylamide gel electrophoresis at ph 8.5, and in the absence of nickel ions, two types of subunit dimers of the nad-linked hydrogenase from nocardia opaca 1b were separated and isolated, and their properties were compared with each other as well as with the properties of the native enzyme. the intact hydrogenase contained 14.3 +/- 0.4 labile sulphur, 13.6 +/- 1.1 iron and 3.8 +/- 0.1 nickel atoms and approximately 1 fmn molecule per enzyme molecule. the oxidized hydrogenase sh ... | 1984 | 6086343 |
| immunoregulatory functions of cultured human t lymphocytes. | twenty continuous cultures of human t cells (ctc) (15 from normal individuals and five from patients with t cell malignancy) growing in the presence of pha-stimulated lymphocyte conditioned medium were studied for their ability to participate in the regulation of the in vitro immunoglobulin (ig) production induced by five polyclonal activators, pokeweed mitogen (pwm), epstein-barr virus (ebv), nocardia opaca water-soluble mitogen (nwsm), streptolysin o (slo), and staphylococcl phage lysate (spl) ... | 1980 | 6264658 |
| effect of nickel on activity and subunit composition of purified hydrogenase from nocardia opaca 1 b. | the nad-reducing hydrogenase of nocardia opaca 1 b was found to be a soluble, cytoplasmic enzyme. n. opaca 1 b does not contain an additional membrane-bound hydrogenase. the soluble enzyme was purified to homogeneity with a yield of 19% and a final specific activity of 45 mumol h2 oxidized min-1 mg protein-1. nad reduction with h2 was completely dependent on the presence of divalent metal ions (ni2+, co2+, mg2+, mn2+) or of high salt concentrations (0.5-1.5 m). the most specific effect was cause ... | 1984 | 6319136 |
| suppression of the late stages of mitogen-induced human b cell differentiation by fc gamma receptors (fc gamma r) released from polymorphonuclear neutrophils. | during short incubation in serum-free medium, polymorphonuclear neutrophils (pmn) release soluble material that can be characterized as receptors for fc igg (fc gamma r) on the following evidence: it agglutinates erythrocyte-igg antibody (eag) complexes, it prevents the binding of eag to eag-rosette-forming cells, and it binds to eag-rosette-forming cells after modulation of their fc gamma r, allowing the formation of 'passive' rosettes. these fc gamma r were isolated by affinity chromatography ... | 1981 | 6456302 |
| modulation of polyclonally activated human peripheral b cells by aggregated igg and by igg-binding factors: differential effect on igg subclass synthesis. | the regulation of igg subclass production by polyclonally activated human b cells was investigated by using two systems previously shown to selectively suppress the generation of igg-containing cells (cc) but not that of igmcc or igacc. the first one involved a brief exposure of peripheral blood mononuclear cells (pbmnc) to heat-aggregated human igg (agg-igg) followed by repeated washings and culture with untreated autologous pbmnc. the second one was achieved by addition of human igg-binding fa ... | 1984 | 6610704 |
| suppression of mitogen-induced peripheral b cell differentiation by soluble fc gamma receptors released from lymphocytes. | soluble receptors for fcigg released from unstimulated human peripheral blood lymphocytes were isolated by affinity chromatography on sepharose 4b-igg. this material was shown to interfere with the differentiation of peripheral blood b cells into ig-secreting cells in cultures stimulated with pokeweek or nocardia opaca extracts. neither cell viability nor [3h]thymidine incorporation were altered, but the number of ig-containing cells and that of ig-secreting cells were decreased. these effects w ... | 1980 | 7007063 |
| antitumor activity of intralesionally administered nocardia opaca preparations in rat and mouse tumors: a comparison with bcg and corynebacterium parvum. | three nocardia products: delipidated cells, lysozyme digest and nocardia water soluble mitogen (nw sm), have been assayed in regression experiments and compared with living bcg and killed c. parvum in rat and mouse fibrosarcomas transplanted intradermally. intratumor injection of these nocardia products induced regression in the bcg sensitive mcfifi2 (s) tumor. these substances were active at a dose of 1 mg, the lysozyme extract and nw sm also being active at 0.1 mg. association with mineral or ... | 1981 | 7021434 |
| oxygen tolerance of strictly aerobic hydrogen-oxidizing bacteria. | growth of various bacteria, especially aerobic hydrogen-oxidizing bacteria, in the presence of 2 to 100% (v/v) oxygen in the gas atmosphere was evaluated. the bacterial strains included alcaligenes eutrophus, a. paradoxus, aquaspirillum autotrophicum, arthrobacter spec. strain 11 x, escherichia coli, arthrobacter globiformis, nocardia opaca, n. autotrophica, paracoccus denitrificans, pseudomonas facilis, p. putida, and xanthobacter autotrophicus. under heterotrophic conditions with fructose or g ... | 1982 | 7049081 |
| [development of hydrogen bacteria on hard surfaces]. | a simple model system stimulating the physical conditions of hydrogen bacterial growth in soil and loose rocks was studied. hydrogen bacterial cells grown under the autotrophic conditions were applied to the surface of coarse-grained silica gel, crushed brick and peat, and constantly supplied with a mixture of h2:o2:co2 (7:2:1). it was shown that nocardia opaca z-766, when cultivated on well-developed solid surfaces with a small quantity of mineral medium, produced an active bacterial film at th ... | 1981 | 7242393 |
| [steroid transforming enzymes in microorganisms. xiii. relationship between steroid structure and induction of 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in nocardia opaca]. | the induction of the 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in nocardia opaca has been shown to be dependent on both hydrophilic and hydrophobic structural features of the steroid molecule, i.g. the nature of the oxygen function in positions 11 and 20 and the presence of the methyl group in position 10 influence the induction of the enzyme drastically. in this connection the metabolism of the steroid inductors being degradated during the induction process has been considered. | 1980 | 7376687 |
| [certain features of the hydrogenase of the hydrogen bacterium nocardia opaca]. | | 1980 | 7392989 |
| immunomodulatory activities of nocardia opaca. | nocardia lysozyme digest (nld), a particulate fraction from nocardia opaca, is able to induce antitumor activity to sal-1 tumor cells (lung sarcoma) in balb/c mice. in mice immunized with nld inhibition of tumor growth and prolonged survival of tumor bearing animals was observed. macrophages isolated from peritoneal cavity and stimulated with nld release a few arachidonic acid metabolites, mostly pge 2. macrophages from tumor bearing mice are more sensitive to nocardia antigens than normal. both ... | 1993 | 7509560 |
| molecular biological analysis of a bidirectional hydrogenase from cyanobacteria. | an 8.9-kb segment with hydrogenase genes from the cyanobacterium anabaena variabilis has been cloned and sequenced. the sequences show homology to the methyl-viologen-reducing hydrogenases from archaebacteria and, even more striking, to the nad(+)-reducing enzymes from alcaligenes eutrophus and nocardia opaca as well as to the nadp(+)-dependent protein from desulfovibrio fructosovorans. the cluster from a. variabilis contains genes coding for both the hydrogenase heterodimer (hoxh and hoxy) and ... | 1995 | 7588754 |
| depression of cytochrome p-450 in mouse liver induced by fractions from nocardia opaca. | we measured the liver cytochrome p-450 content of mice 24 h after they had been injected with the following immunoadjuvants: nocardia opaca derivatives and peptidoglycans from several bacterial strains. the cell wall fraction was not active, the others diminished liver cytochrome p-450 levels. the dose-response activity varied with the bacterial origin of the peptidoglycans. these findings indicate that the toxicity and efficiency of immunochemotherapeutic protocols can be modified by altering d ... | 1994 | 7868300 |
| improved purification of steroid 1:2-dehydrogenase from nocardia opaca and partial characterization of its cloned gene sequence. | we have purified a steroid-inducible 1:2-dehydrogenase from nocardia opaca. the final enzyme preparation was purified 120-fold with a recovery of 38%. the n-terminal amino acid sequence was determined to be: met-gln-asp-trp-thr-ser-glu-(cys)-asp-val-leu-val-val-gly-. from the genomic library of nocardia opaca in the plasmid puc19, a clone designated as pstd23 containing a 0.9 kb kpni-psti fragment was found to hybridize with an oligonucleotide probe corresponding to the first six amino acids fro ... | 1993 | 7916596 |
| immunomodulation by bacterial fractions. | the cells of nocardia opaca are a source of potent immunostimulating substances, differing in solubility and in the presence or absence of peptidoglycan. three classes of n. opaca fractions have been investigated: (1) ndcm (nocardia delipidated cell mitogen), the starting fraction; (2) pg (peptidoglycan)-containing fractions: cw (cell walls), pg, and fractions containing soluble pg derivatives such as nwsm (nocardia water soluble mitogen) and nspd (nocardia soluble peptidoglycan derivative); and ... | 1994 | 7927995 |
| antitumoral activity of an immunomodulatory fraction of nocardia opaca: mechanism of action. | immunomodulatory substances have been used as antineoplastic agents in experimental and human systems. many of these agents were derived from microorganisms. several biologically active fractions have been isolated from nocardia. these derivatives were shown to induce interferon production, to activate natural killer cells and macrophages and to exert an antitumoral effect. we attempted to examine the mechanism of the antitumoral activity of the nocardia water-soluble mitogen (nwsm). the tumor t ... | 1994 | 7927996 |
| treatment of radiation disease by nocardia fraction: possible effect of inflammatory cytokines. | nocardia delipidated cell mitogen (ndcm) derived from nocardia opaca, given 2 h after whole-body gamma irradiation (2.5 gy) to germ-free piglets in amounts of 1 mg/kg via a stomach tube, prevented the loss of b-cells in the spleen, intestinal mucosa, and mesenteric lymph nodes when recorded on day 8 after irradiation. ndcm and/or formolized enterococcus faecalis strain (ef) applied intraperitoneally also 2 h after irradiation increased the survival rate in mice irradiated with 8.3 gy and 8.5 gy ... | 1994 | 7927997 |
| induction of inflammatory cytokines by nocardia fractions. | ndcm and nld fractions of nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. inflammatory cytokines il-1, il-6, tumour necrosis factor (tnf) alpha and interferon (ifn) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. il-1, il-6 and tnf were produced by human monocytes stimulated for 2 h ... | 1993 | 8206169 |
| the giant linear plasmid phg207 from rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini. | as described previously, in rhodococcus sp. (formerly nocardia opaca) strains mr11 and mr22, the ability to grow as an aerobic hydrogen bacterium (the aut character) is located on giant conjugative linear plasmids--on phg201 (270 kb) in strain mr11 and on phg205 (280 kb) in strain mr22. in an autotrophic transconjugant originating from mr22 a smaller plasmid, phg207 (225 kb), was detected and shown to be a recombination product of the wild-type plasmids phg204 and phg205. a donor carrying phg207 ... | 1993 | 8245832 |
| changes in distribution of lectin receptors in macrophages activated by nocardia water soluble molecules. | lectin-histochemical studies were performed on macrophages activated by nocardia opaca water soluble molecules (nwsm) and elicited by phosphate buffered saline (pbs) as control to demonstrate the pattern of carbohydrate residues. ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (abc) was used as visualizer. the lectins wheat germ agglutinin (wga) and griffonia simplicifolia agglutinin-i (gs-i) stained macrophages only after activation by nwsm and not ma ... | 1993 | 8298432 |
| nocardia fractions, nld and nwsm, induce tumor necrosis factor-alpha secretion in human monocytes: role of protein kinase c. | nocardia lysozyme digest (nld) and nocardia water-soluble mitogen (nwsm) are two fractions derived from nocardia opaca. in this report, we demonstrated that both fractions elicited significant secretion of tumor necrosis factor-alpha (tnf-alpha) in human monocytes. supernatants from monocytes stimulated with nwsm and low concentrations of nld displayed a cytotoxic activity against tnf-alpha-sensitive l929 cells, but supernatants from monocytes stimulated with high concentrations of nld failed to ... | 1993 | 8325335 |
| complete remission of metastatic breast cancer by non-specific immunostimulation with a nocardia opaca cell-wall extract after failure of conventional therapy. | in a male patient with recurring lung metastases of infiltrating breast carcinoma (t2nomo), primary treatment mastectomy, axillar dissection and local irradiation) the tumor metastases developed progressively despite of repeated surgical removement, polychemotherapy, and antiestrogen treatment. active unspecific immunotherapy with a nocardia opaca cell-wall extract, started in the final stage of the disease with deterioration of the performance status, resulted within several months in a complet ... | 1994 | 8571066 |
| macrophage nitric oxide synthase (nos) activation by nocardia opaca fractions and 15- and 56-kd isolated antigens. | the gram-positive bacterium, nocardia opaca, is a source of substances with adjuvant effect, ability to stimulate macrophages and natural killer cells for enhanced cytotoxity and cytokine production and b lymphocytes for polyclonal immunoglobulin secretion. we determined the immunogenicity of isolated n. opaca fractions and prepared moabs against immunogenic water-soluble mitogen (nwsm). two main proteins of molecular mass 15 and 56 kd were detected in western blot analysis and isolated by affin ... | 1996 | 8625511 |
| formation of intracytoplasmic lipid inclusions by rhodococcus opacus strain pd630. | an oleaginous hydrocarbon-degrading rhodococcus opacus strain (pd630) was isolated from a soil sample. the cells were able to grow on a variety of substrates and to produce large amounts of three different types of intracellular inclusions during growth on alkanes, phenylalkanes, or non-hydrocarbon substrates. electron microscopy revealed large numbers of electron-transparent inclusions with a sphere-like structure. in addition, electron-dense inclusions representing polyphosphate and electron-t ... | 1996 | 8661931 |
| expression of tnf-alpha in pig fetal cells stimulated in vitro. | macrophages and lymphocytes of pig fetuses stimulated in vitro with bacterial mitogens such as lipopolysaccharide and nocardia opaca delipidated cell mitogen showed a high tnf-alpha cytoplasmic expression. tnf-alpha was detected by immunofluorescence in peripheral blood lymphocytes and lymphocytes from the thymic region as early as at 34 d of gestation. macrophages were the main producers of tnf-alpha at later developmental stages. | 1995 | 8763156 |
| simultaneous utilization of pyridine and fructose by rhodococcus opacus ufz b 408 without an external nitrogen source. | a bacterium classified as rhodococcus opacus, which is able to use pyridine (a potentially growth-inhibiting substrate) as its sole source of carbon, energy and nitrogen, was isolated. in a carbon-limited chemostat culture, the kinetics was determined for growth on both pyridine and a mixture of pyridine and fructose (9 mm/22.15 mm). with growth on pyridine, stable steady states were achieved up to dilution rates of about 0.1 h-1. a further increase in the dilution rate resulted in the progressi ... | 1996 | 8920194 |
| flow cytometric study of the epirubicine accumulation modulation in the human erythrocytes by a nocardia opaca fraction. | the effect of the "nocardia lysozyme digest" (nld) obtained from nocardia opaca on the epirubicine accumulation in erythrocytes was studied by flow cytometry. it was shown that the number of fluorescent cells increased under the nld action due to the increased membrane permeability for epirubicine. an opposite effect was also recorded consisting in the decrease of the relative fluorescence intensity per cell. this effect was attributed to the fluorescence quenching by the oxygen metabolites prod ... | 1995 | 8993113 |
| modulating the expression of some biological membrane glycoconjugates by a nocardia opaca fraction. | by some hemagglutination (ha) studies it was proved that nld (the lysozyme digest fraction of nocardia opaca) interacts with the sendai virus envelope glycoproteic receptors, but not with those of the beijing 353/89 h3n2) influenza virus. very likely the inhibition is due to the presence of some gal lectins in nld: the erythrocytes agglutinability (by the sendai virus and by the beijing 353/89 h3n2) influenza virus is enhanced by their incubation with galactose. | 1995 | 8993122 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22 | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9042758 |
| acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes. | aerobic acetylene-degrading bacteria were isolated from soil samples. two isolates were assigned to the species rhodococcus opacus, two others to rhodococcus ruber and gordona sp. they were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. the acetylene hydratases of r opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(iii) citrate. expression of these enzymes was molybdenum-dependent. acetylene hydrat ... | 1997 | 9084145 |
| occurrence and specificity of human natural and in vitro induced antibodies to nocardia opaca antigens. | nocardia opaca, a gram-positive bacterium, is a potent source of immunostimulatory substances. screening of sera of adult human donors revealed that all sera tested contained antibodies reactive with isolated nocardia fractions (nocardia delipidated cell mitogen, ndcm; nocardia lysozyme digest, nld; nocardia water-soluble mitogen, nwsm; and fraction b). the respective values of reciprocal titres for igm and igg were in the range of 100 to 12,800, and 10 to 320 for iga antibody isotypes, when nld ... | 1996 | 9089009 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22. | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9133325 |
| genes encoding the nad-reducing hydrogenase of rhodococcus opacus mr11. | the dissociation of the soluble nad-reducing hydrogenase of rhodococcus opacus mr11 into two dimeric proteins with different catalytic activities and cofactor composition is unique among the nad-reducing hydrogenases studied so far. the genes of the soluble hydrogenase were localized on a 7.4 kbp asnl fragment of the linear plasmid phg201 via heterologous hybridization. analysis of the nucleotide sequence of this fragment revealed the seven open reading frames orf1, hoxf, -u, -y, -h, -w and orf7 ... | 1997 | 9141690 |
| [jomol--immunostimulation and rejection of tumor cells?]. | jomol contains cell wall fragments of the bacterium nocardia opaca composed of 40% oligopeptides, 40% lipids, 10-15% polysaccharides, and 5-10% peptidoglycans. technetium labeled--jomo tech--and indium-labeled--jomo in--preparations are used for diagnosing cancer. jomol is promoted to cure any cancer, except non-hodgkin lymphoma and ovarian cancer. it is claimed that jomol has no side effects, however chills and high fever have been observed. following initial intensive therapy with daily inject ... | 1997 | 9312814 |
| characterization of a protocatechuate catabolic gene cluster from rhodococcus opacus 1cp: evidence for a merged enzyme with 4-carboxymuconolactone-decarboxylating and 3-oxoadipate enol-lactone-hydrolyzing activity. | the catechol and protocatechuate branches of the 3-oxoadipate pathway, which are important for the bacterial degradation of aromatic compounds, converge at the common intermediate 3-oxoadipate enol-lactone. a 3-oxoadipate enol-lactone-hydrolyzing enzyme, purified from benzoate-grown cells of rhodococcus opacus (erythropolis) 1cp, was found to have a larger molecular mass under denaturing conditions than the corresponding enzymes previously purified from gamma-proteobacteria. sequencing of the n ... | 1998 | 9495744 |
| evolutionary relationship between chlorocatechol catabolic enzymes from rhodococcus opacus 1cp and their counterparts in proteobacteria: sequence divergence and functional convergence. | biochemical investigations of the muconate and chloromuconate cycloisomerases from the chlorophenol-utilizing strain rhodococcus opacus (erythropolis) 1cp had previously indicated that the chlorocatechol catabolic pathway of this strain may have developed independently from the corresponding pathways of proteobacteria. to test this hypothesis, we cloned the chlorocatechol catabolic gene cluster of strain 1cp by using pcr with primers derived from sequences of n termini and peptides of purified c ... | 1998 | 9495745 |
| computation of the electrical double layer properties of semipermeable membranes in multicomponent electrolytes | a methodology is presented for calculating of the surface potential, donnan potential, and ion concentration profiles for semipermeable microbial membranes that is valid for an arbitrary electrolyte composition. this model for surface potential, donnan potential, and charge density was applied to recently reported experimental data for gram-positive bacteria, including bacillus brevis, rhodococcus opacus, rhodococcus erythropolis, and corynebacterium species. these calculations show that previou ... | 1998 | 9603855 |
| the terminal structures of linear plasmids from rhodococcus opacus. | the telomers of several linear plasmids of rhodococcus opacus (formerly nocardia opaca) were studied. the plasmids phg201, phg204 and phg205 carry proteins bound to their ends, as shown by gel retardation experiments. a sequence hybridizing with the terminal sequence of phg207, a recombinant linear plasmid consisting of the left part of phg204 and the right part of phg205, which was analysed in a previous study by the authors, could be detected in all linear plasmids of the wild-type r. opacus s ... | 1998 | 9611802 |
| characterization of the maleylacetate reductase maca of rhodococcus opacus 1cp and evidence for the presence of an isofunctional enzyme. | maleylacetate reductases (ec 1.3.1.32) have been shown to contribute not only to the bacterial catabolism of some usual aromatic compounds like quinol or resorcinol but also to the degradation of aromatic compounds carrying unusual substituents, such as halogen atoms or nitro groups. genes coding for maleylacetate reductases so far have been analyzed mainly in chloroaromatic compound-utilizing proteobacteria, in which they were found to belong to specialized gene clusters for the turnover of chl ... | 1998 | 9657989 |
| immunomodulatory properties of nocardia lysozyme digest (nld) in complement normal and c5-deficient mice. | the constantly increasing number of substances with adjuvant activity outpaces the elucidation of their mode of action. this problem is of great importance as the immunomodulatory action of an adjuvant is time- and route-dependent, which implies that administration at a different moment or site may result in a reduced immune response. in the present work the possibility to achieve dual effect (stimulatory or inhibitory) is regarded in the light of the complement system. the object of the study i ... | 1998 | 9682384 |
| microcosm enrichment of biphenyl-degrading microbial communities from soils and sediments. | a microcosm enrichment approach was employed to isolate bacteria which are representative of long-term biphenyl-adapted microbial communities. growth of microorganisms was stimulated by incubating soil and sediment samples from polluted and nonpolluted sites with biphenyl crystals. after 6 months, stable population densities between 8 x 10(9) and 2 x 10(11) cfu/ml were established in the microcosms, and a large percentage of the organisms were able to grow on biphenyl-containing minimal medium p ... | 1998 | 9687466 |
| 3-nitroadipate, a metabolic intermediate for mineralization of 2, 4-dinitrophenol by a new strain of a rhodococcus species. | the bacterial strain rb1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16s rrna gene sequence comparison, as a rhodococcus species closely related to rhodococcus opacus. rhodococcus sp. strain rb1 degrades 2,4-dinitrophenol, releasing the two nitro groups from the compound as nitrite. the release of nitro groups from 2,4-dinitrophenol occurs in two steps. first, the 2-nitro group is removed as ... | 1999 | 9864324 |
| a sensitive, viable-colony staining method using nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. | the oxazine dye nile blue a and its fluorescent oxazone form, nile red, were used to develop a simple and highly sensitive staining method to detect poly(3-hydroxybutyric acid) and other polyhydroxyalkanoic acids (phas) directly in growing bacterial colonies. in contrast to previously described methods, these dyes were directly included in the medium at concentrations of only 0.5 microgram/ml, and growth of the cells occurred in the presence of the dyes. this allowed an estimation of the presenc ... | 1999 | 9914303 |
| effect of aromatic compounds on cellular fatty acid composition of rhodococcus opacus. | in cells of rhodococcus opacus gm-14, gm-29, and 1cp, the contents of branched (10-methyl) fatty acids increased from 3% to 15 to 34% of the total fatty acids when the cells were grown on benzene, phenol, 4-chlorophenol, chlorobenzene, or toluene as the sole source of carbon and energy, in comparison with cells grown on fructose. in addition, the content of trans-hexadecenoic acid increased from 5% to 8 to 18% with phenol or chlorophenol as the carbon source. the 10-methyl branched fatty acid co ... | 1999 | 9925629 |
| the putative regulator of catechol catabolism in rhodococcus opacus 1cp--an iclr-type, not a lysr-type transcriptional regulator. | the catechol catabolic genes catabc from rhodococcus opacus 1cp have previously been characterized by sequence analysis of the insert cloned on plasmid prer1. now, a 5.1-kb dna fragment which overlaps with the insert of prer1 was cloned, yielding prer2, and subjected to sequencing. besides three other open reading frames, a gene was detected ca 200 bp upstream of the catechol 1,2-dioxygenase gene cata, which is obviously transcribed divergently from catabc. the protein which can be deduced from ... | 1998 | 10068790 |
| linear alkanesulfonates as carbon and energy sources for gram-positive and gram-negative bacteria. | several bacteria from soil and rainwater samples were enriched and isolated with propanesulfonate or butanesulfonate as sole carbon and energy source. most of the strains isolated utilized nonsubstituted alkanesulfonates with a chain length of c3-c6 and the substituted sulfonates taurine and isethionate as carbon and energy source. a gram-positive isolate, p40, and a gram-negative isolate, p53, were characterized in more detail. phylogenetic analysis grouped strain p40 within group iv of the gen ... | 1999 | 10369899 |
| preferential oxidative dehalogenation upon conversion of 2-halophenols by rhodococcus opacus 1g. | the regiospecificity of hydroxylation of c2-halogenated phenols by rhodococcus opacus 1g was investigated. oxidative defluorination at the c2 position ortho with respect to the hydroxyl moiety was preferred over hydroxylation at the non-fluorinated c6 position for all 2-fluorophenol compounds studied. initial hydroxylation of 2,3, 5-trichlorophenol resulted in the exclusive formation of 3, 5-dichlorocatechol. these results indicate that, in contrast to all other phenol ortho-hydroxylases studied ... | 1999 | 10564791 |
| establishment of a gene transfer system for rhodococcus opacus pd630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids). | a gene transfer system for rhodococcus opacus pd630 based on electroporation was established and optimized employing the escherichia coli-rhodococcus shuttle vectors pnc9501 and pnc9503 as well as the e. coli-corynebacterium glutamicum shuttle vector pjc1 as suitable cloning vectors for r. opacus pd630, resulting in transformation efficiencies up to 1.5 x 10(5) cfus/microgram plasmid dna. applying the optimized electroporation protocol to the pnc9501-derivatives pak68 and pak71 harboring the ent ... | 1999 | 10570798 |
| characterization of the protocatechuic acid catabolic gene cluster from streptomyces sp. strain 2065. | protocatechuate 3,4-dioxygenase (ec 1.13.11.3) catalyzes the ring cleavage step in the catabolism of aromatic compounds through the protocatechuate branch of the beta-ketoadipate pathway. a protocatechuate 3,4-dioxygenase was purified from streptomyces sp. strain 2065 grown in p-hydroxybenzoate, and the n-terminal sequences of the beta- and alpha-subunits were obtained. pcr amplification was used for the cloning of the corresponding genes, and dna sequencing of the flanking regions showed that t ... | 2000 | 10742233 |
| characterization of the naphthalene-degrading bacterium, rhodococcus opacus m213. | bacterial strain m213 was isolated from a fuel oil-contaminated soil in idaho, usa, by growth on naphthalene as a sole source of carbon, and was identified as rhodococcus opacus m213 by 16s rdna sequence analysis and growth on substrates characteristic of this species. m213 was screened for growth on a variety of aromatic hydrocarbons, and growth was observed only on simple 1 and 2 ring compounds. no growth or poor growth was observed with chlorinated aromatic compounds such as 2,4-dichloropheno ... | 2000 | 10754253 |
| identification of fluoropyrogallols as new intermediates in biotransformation of monofluorophenols in rhodococcus opacus 1cp. | the transformation of monofluorophenols by whole cells of rhodococcus opacus 1cp was investigated, with special emphasis on the nature of hydroxylated intermediates formed. thin-layer chromatography, mass spectrum analysis, and (19)f nuclear magnetic resonance demonstrated the formation of fluorocatechol and trihydroxyfluorobenzene derivatives from each of three monofluorophenols. the (19)f chemical shifts and proton-coupled splitting patterns of the fluorine resonances of the trihydroxyfluorobe ... | 2000 | 10788394 |
| [dependence of transformation of chlorophenols by rhodococci on position and number of chlorine atoms in the aromatic ring]. | study of the conversion of chlorophenols by rhodococcus opacus 1g, r. rhodnii 135, r. rhodochrous 89, and r. opacus 1cp disclosed the dependence of the conversion rate and pathway on the number and position of chlorine atoms in the aromatic ring. the most active chlorophenol converter, strain r. opacus 1cp, grew on each of the three isomeric monochlorophenols and on 2,4-dichlorophenol; the rate of growth decreased from 4-chlorophenol to 3-chlorophenol and then to 2-chlorophenol. the parameters o ... | 2000 | 10808489 |
| rhodococcus opacus strain pd630 as a new source of high-value single-cell oil? isolation and characterization of triacylglycerols and other storage lipids. | the triacylglycerol (tag)-accumulating, hydrocarbon-degrading bacterium rhodococcus opacus strain pd630 and chemically induced storage-deficient mutants derived from this strain were investigated for their capability to accumulate storage lipids in the cytoplasm during cultivation under nitrogen-limiting conditions. acylglycerols were analysed by matrix-associated laser desorption ionization-time of flight mass spectrometry (maldi-tof) and by reversed-phase hplc. fatty acids comprising 13-19 car ... | 2000 | 10832641 |
| accumulation and mobilization of storage lipids by rhodococcus opacus pd630 and rhodococcus ruber ncimb 40126. | the time course of the accumulation of triacylglycerols (tags) in rhodococcus opacus pd630 or of tags plus polyhydroxyalkanoates (pha) in rhodococcus ruber ncimb 40126 with gluconate or glucose as carbon source, respectively, was studied. in addition, we examined the mobilization of these storage compounds in the absence of a carbon source. r. opacus accumulated tags only after the exhaustion of ammonium in the medium, and, with a fixed concentration of the carbon source, the amounts of tags in ... | 2000 | 10968636 |
| in vitro effects of sterculic acid on lipid biosynthesis in rhodococcus opacus strain pd630 and isolation of mutants defective in fatty acid desaturation. | the in vivo effects of sterculic acid methyl ester on triacylglycerol fatty acid composition in the oleaginous, hydrocarbon-degrading bacterium r. opacus strain pd630 was investigated. sterculic acid, a cyclopropene fatty acid and an inhibitor of the stearoyl-coa desaturase system, strongly inhibited the synthesis of monoenic fatty acids, of saturated fatty acids with more than 16 carbon atoms and of odd-numbered fatty acids when added to the culture medium. in addition, chemical mutagenesis and ... | 2000 | 10981688 |
| [growth of rhodococcus opacus on mixtures of monohalogenated benzenes and phenols]. | the growth of rhodococcus opacus gm-14 on mixtures of 2-chloro- and 2-bromophenol, of 4-chloro, 4-bromo-, and 4-iodophenol, and of chloro-, bromo-, and iodobenzenes was accompanied by consumption of the substrates and excretion of halogen ions to the medium. during the growth on monochlorophenols, the substrates were consumed sequentially in the following order: 4-chloro-, 3-chloro-, and then 2-chlorophenol. chlorine ions were excreted in a two-phase manner in amounts comprising 79% of the theor ... | 2000 | 11008684 |