| 'candidatus phytoplasma omanense', associated with witches'-broom of cassia italica (mill.) spreng. in oman. | samples from plants of cassia italica exhibiting typical witches'-broom symptoms (cassia witches'-broom; cwb) were examined for the presence of plant pathogenic phytoplasmas by pcr amplification using universal phytoplasma primers. all affected plants yielded positive results. rflp analyses of rrna gene products indicated that the phytoplasmas detected were different from those described previously. phylogenetic analysis of 16s rrna gene sequences confirmed that cwb represents a distinct lineage ... | 2008 | 18218949 |
| a phytoplasma closely related to the pigeon pea witches'-broom phytoplasma (16sr ix) is associated with citrus huanglongbing symptoms in the state of são paulo, brazil. | in february 2007, sweet orange trees with characteristic symptoms of huanglongbing (hlb) were encountered in a region of são paulo state (sps) hitherto free of hlb. these trees tested negative for the three liberibacter species associated with hlb. a polymerase chain reaction (pcr) product from symptomatic fruit columella dna amplifications with universal primers fd1/rp1 was cloned and sequenced. the corresponding agent was found to have highest 16s rdna sequence identity (99%) with the pigeon p ... | 2008 | 18943735 |
| characterization and molecular differentiation of 16sri-e and 16srix-e phytoplasmas associated with blueberry stunt disease in new jersey. | a nested pcr assay was employed to detect the presence of phytoplasmas in 127 blueberry plants exhibiting typical or a portion of blueberry stunt (bbs) syndrome collected in 2010 and 2011, from 11 commercial farms predominantly located in two counties in new jersey, usa. ninety plants exhibiting typical stunt syndrome tested positive for phytoplasma infection. restriction fragment length polymorphism (rflp) analysis indicated that two distinct phytoplasmas were associated with bbs-diseased plant ... | 2013 | 23195601 |
| differentiation and classification of phytoplasmas in the pigeon pea witches'-broom group (16srix): an update based on multiple gene sequence analysis. | the pigeon pea witches'-broom phytoplasma group (16srix) comprises diverse strains that cause numerous diseases in leguminous trees and herbaceous crops, vegetables, a fruit, a nut tree and a forest tree. at least 14 strains have been reported worldwide. comparative phylogenetic analyses of the highly conserved 16s rrna gene and the moderately conserved rplv (rpl22)-rpsc (rps3) and secy genes indicated that the 16srix group consists of at least six distinct genetic lineages. some of these lineag ... | 2012 | 22307512 |