| effects of herbs and drugs during pregnancy and lactation. | prior to considering the effects of herbs and drugs during pregnancy and lactation, attention is directed to the relevance of herbal medicines to human patients. when a drug is taken orally, its absorption, biotransformation, and excretion will affect its blood level. it then makes its way to the capillaries surrounding the alveoli of the breast, and the drug must cross the endothelium of the capillaries into the alveolar cells of the breast and then be excreted into the lumen with the milk. ... | 1979 | 262485 |
| antiplatelet actions of some coumarin compounds isolated from plant sources. | xanthyletin, xanthoxyletin, suberosin (all from citrus grandis), aurapten (from severinia huxifolia) and poncitrin (from poncirus trifoliata) were isolated and their chemical structures were characterized to be coumarin compounds. all these coumarin compounds except xanthyletin inhibited the aggregation and atp release of rabbit platelets induced by arachidonic acid, collagen, adp, platelet-activating factor (paf) or u46619 (a thromboxane a2 analog). thrombin-induced atp release, but not the agg ... | 1992 | 1523611 |
| an evaluation of sequence tagged microsatellite site markers for genetic analysis within citrus and related species. | microsatellites, also called sequence tagged microsatellite sites (stmss), have become important markers for genome analysis but are currently little studied in plants. to assess the value of stmss for analysis within the citrus plant species, two example stmss were isolated from an intergeneric cross between rangpur lime (citrus x limonia osbeck) and trifoliate orange (poncirus trifoliata (l.) raf.). unique flanking primers were constructed for polymerase chain reaction amplification both withi ... | 1995 | 7774802 |
| interspecific somatic hybrid of ichang papeda with valencia orange. | protoplasts from the leaf of ichang papeda (citrus ichangensis swingle) were fused with the protoplasts of embryogenic suspension culture of valencia orange (citrus sinensis osbeck) in vitro by polyethylene glycol (peg)-induced fusion. the regenerated embryoids were malformed and were transferred onto shoot induction medium. the shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18 ... | 1993 | 8199318 |
| the persistence of engineered agrobacterium tumefaciens in agroinfected plants. | several plant species, including tomato (lycopersicon esculentum), gynura aurantiaca, avocado (persea americana), and grapefruit (citrus paradisi) grafted on troyer citrange (poncirus trifoliata x c. sinensis) were "agro-infected" with agrobacterium tumefaciens strain lba-4404, carrying a mini-ti plasmid with a dimeric cdna of citrus exocortis viroid (cevd). extracts prepared from tissues of the agroinfected plants 38-90 days after inoculation were plated on selective media and found to contain ... | 1993 | 8274776 |
| antianaphylactic activity of poncirus trifoliata fruit extract. | the effect of an aqueous extract of poncirus trifoliata (l.) raf. (rutaceae) fruits (ptfe) on compound 48/80-induced mortality associated with anaphylaxis was studied in rats. ptfe inhibited compound 48/80-induced anaphylaxis 100% with a dose of 1.6 mg/g body weight (bw) 1 h before or 5 min after injection of compound 48/80. ptfe inhibited compound 48/80-induced anaphylaxis almost 100% with doses above 0.4 mg/g bw intraperitoneally administered. ptfe (1-1000 micrograms/ml) also dose-dependently ... | 1996 | 8953421 |
| effects of poncirus trifoliata on type i hypersensitivity reaction. | a study was carried out to examine the effect of an aqueous extract from immature fruit of poncirus trifoliata l. (rutaceae) (ptife) on the type i hypersensitivity reaction. forty-eight hour pca (passive cutaneous anaphylaxis) in rats was significantly inhibited by the oral administration of ptife (200 mg/kg). it also inhibited histamine release from rat peritoneal mast cells (rpmc) induced by mouse anti-dinitrophenyl (dnp)-ige and dinitrophenyl-human serum albumin (dnp-hsa). these results sugge ... | 1997 | 9166997 |
| a high-resolution linkage map of the citrus tristeza virus resistance gene region in poncirus trifoliata (l.) raf. | resistance to citrus tristeza virus (ctv) was evaluated in 554 progeny of 10 populations derived from poncirus trifoliata. a dominant gene (ctv) controlled ctv resistance in p. trifoliata. twenty-one dominant pcr-based dna markers were identified as linked to ctv by bulked segregant analysis. of the 11 closest markers to ctv, only 2 segregated in all populations. ten of these markers were cloned and sequenced, and codominant rflp markers were developed. seven rflp markers were then evaluated in ... | 1998 | 9755216 |
| relationship between volatile components of citrus fruit essential oils and antimicrobial action on penicillium digitatum and penicillium italicum. | this study examined the effect of volatile components of citrus fruit essential oils on p. digitatum and p. italicum growth. the hydrodistilled essential oils of orange (citrus sinensis cvv. "washington navel", "sanguinello", "tarocco", "moro", "valencia late", and "ovale"), bitter (sour) orange (c. aurantium), mandarin (c. deliciosa cv. "avana"), grapefruit (c. paradisi cvv. "marsh seedless" and "red blush"), citrange (c. sinensis x poncirus trifoliata cvv. "carrizo" and "troyer"), and lemon (c ... | 1998 | 9761340 |
| anti-helicobacter pylori activity of the metabolites of poncirin from poncirus trifoliata by human intestinal bacteria. | poncirin was isolated from water extract of the fruits of poncirus trifoliata and metabolized by human intestinal bacteria. the inhibitory effect of poncirin and its metabolites by these bacteria on the growth of helicobacter pylori (hp) was investigated. among them, ponciretin (5,7-dihydroxy-4'-methoxyflavanone), the main metabolite most potently inhibited the growth of hp, with a minimum inhibitory concentration (mic) of 10-20 microg/ml. however, poncirin and its metabolites except ponciretin ... | 1999 | 10328566 |
| inhibition of immunoglobulin e production by poncirus trifoliata fruit extract. | immunoglobulin e (ige) is the principal immunoglobulin involved in immediate hypersensitivities and chronic allergic diseases. the effect of an aqueous extract of poncirus trifoliata (l) raf. (rutaceae) fruits (ptfe) on in vivo and in vitro ige production was investigated. ptfe dose-dependently inhibited the active systemic anaphylaxis and serum ige production induced by immunization with ovalbumin, bordetella pertussis toxin and aluminum hydroxide gel. ptfe strongly inhibited interleukin 4 (il- ... | 1999 | 10473174 |
| identification of sources of plant resistance to diaprepes abbreviatus (coleoptera: curculionidae) by three bioassays. | host plant resistance to the root weevil diaprepes abbreviatus (l.) was assessed for 3 citrus rootstock cultivars, 5 promising hybrid rootstocks, and 3 citroid fruit trees using 3 bioassay methods: a pot bioassay with 1-yr seedlings; a new, 21-cm plastic cell bioassay with 5-mo seedlings; and a diet incorporation bioassay. the plastic cell bioassay is a more rapid screening method and is capable of evaluating a larger number of entries in a shorter period compared with current methods. the 3 bio ... | 1999 | 10504899 |
| larval growth of diaprepes abbreviatus (coleoptera: curculionidae) and resulting root injury to three citrus varieties in two soil types. | larval growth and intraspecific competition of diaprepes abbreviatus (l.) larvae and consequent root injury in container-grown citrus in the greenhouse were evaluated. roots of carrizo citrange, citrus sinesis l. osbeck x poncirus trifoliata (l.) raf.; cleopatra mandarin, c. reticulata blanco, and swingle citrumelo, c. paradisi macf. x p. trifoliata (l.) raf. rootstock seedlings grown in candler fine sand and potting soil were colonized with different populations of d. abbreviatus larvae. larvae ... | 2000 | 10826189 |
| characterization of the low affinity transport system for no(3)(-) uptake by citrus roots. | three-month old citrange troyer (hybrid of citrus sinensis x poncirus trifoliata) seedlings were grown hydroponically and, after a period of no(3)(-) starvation, plants were transferred to solutions enriched with k(15)no(3) (96% atoms 15n excess) to measure 15no(3)(-) uptake rates as a function of external 15no(3)(-) concentrations. two different no(3)(-) uptake systems were found. between 1 and 50 mm 15no(3)(-) in the uptake solution medium, the uptake rate increased linearly due to the low aff ... | 2000 | 11164581 |
| construction of a 1.2-mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of poncirus trifoliata (l.) raf. | the citrus tristeza virus resistance gene (ctv) is a single dominant gene in poncirus trifoliata, a sexually compatible relative of citrus. to clone this gene, a bacterial artificial chromosome (bac) library has been constructed from an individual plant that was homozygous for ctv. this library contains 45,696 clones with an average insert size of 80 kb, corresponding to 9.6 genome equivalents. screening of the bac library with five chloroplast dna probes indicated that 0.58% of the bac clones c ... | 2001 | 11444697 |
| fine genetic mapping and bac contig development for the citrus tristeza virus resistance gene locus in poncirus trifoliata (raf.). | a map-based cloning strategy has been employed to isolate ctv, a single dominant gene from poncirus trifoliata that confers resistance to citrus tristeza virus (ctv), the most important viral pathogen of citrus. cloning of this gene will allow development of commercially acceptable, virus-resistant cultivars. a high-resolution genetic linkage map of the ctv locus region was developed using a backcross population of 678 individuals. three dna markers that were closely linked or co-segregated with ... | 2001 | 11459195 |
| [inheritance of organelle genomes of the somatic hybrid between cleopatra mandarin (citrus reticulata) and flying dragon (poncirus trifoliata)]. | cleaved amplified polymorphic sequence (caps) was successfully applied to analyze the organelle composition of three eight-year-old trees of the somatic hybrid between cleopatra mandarin (citrus reticulata) and flying dragon (poncirus trifoliata). five chloroplast and five mitochondrial universal primer pairs were used. all chloroplast primer pairs (rbcl-rbcl, rbcl-psa i, trnh-trnk, trnd-trnt, trnk-trnk) and three (nad 1 exon b-nad 1 exon c, 18s rrna-5s rrna, nad 4 exon 1-nad 4 exon 2) of the fi ... | 2002 | 11985273 |
| identification of a grapefruit cdna belonging to a unique class of citrus dehydrins and characterization of its expression patterns under temperature stress conditions. | citrus fruits are sensitive to low temperatures and this often results in the development of chilling injuries during postharvest storage. in order to gain more insight into the molecular mechanisms involved in the acquisition of fruit chilling tolerance, we initiated a grapefruit (citrus paradisi, cv. marsh seedless) flavedo cdna sequencing project and used it to identify a cdna similar to other poncirus trifoliata and citrus unshiu dehydrin genes reported to be responsive to low temperatures. ... | 2002 | 12121466 |
| inheritance of organelle dna sequences in a citrus-poncirus intergeneric cross. | many land plants deviate from the maternal pattern of organelle inheritance. in this study, heterologous mitochondrial and chloroplast probes were used to investigate the inheritance of organelle genomes in the progeny of an intergeneric cross. the seed parent was lb 1-18 (a hybrid of citrus reticulata blanco cv. clementine x c. paradisi macf. cv. duncan) and the pollen parent was the cross-compatible species poncirus trifoliata (l.) raf. all 26 progeny examined exhibited maternal inheritance of ... | 2002 | 12195032 |
| [studies on chinese drugs zhiqiao and zhishi origin investigation and merchandise identification]. | to sort out the main original plants of chinese drugs zhiqiao and zhishi. | 1999 | 12205880 |
| [factors affecting agrobacterium-mediated genetic transformation of poncirus trifoliata (l.) raf]. | establishing an effective system for the agrobacterium-mediated genetic transformation of poncirus trifoliata, so as to lay a foundation for improving breeds and introducing foreign genes. | 1999 | 12242794 |
| is there meaningful plant resistance to diaprepes abbreviatus (coleoptera: curculionidae) in citrus rootstock germplasm? | host plant resistance to the diaprepes root weevil, diaprepes abbreviatus (l.) was assessed for seedlings of 54 poncirus trifoliata (l.) raf. selections and two families of progeny from crosses between citrus and p. trifoliata. weight gain was consistently lower when larvae were reared in pots containing the progeny of citrus reticulata blanco 'sunki' x p. trifoliata 'flying dragon' compared with larvae reared on progeny of 'pearl' (c. reticulata x c. paradisi macf.) x 'flying dragon'. this is t ... | 2002 | 12403435 |
| effects of rootstock on the composition of bergamot (citrus bergamia risso et poiteau) essential oil. | this paper reports the composition of bergamot oils obtained from plants grafted on the following rootstocks: sour orange, carrizo citrange, trifoliate orange, alemow, volkamerian lemon, and troyer citrange. the aim of this study is to evaluate the possibility of using rootstocks other than sour orange, checking their effect on the composition of the essential oil. results are reported for analysis of 203 bergamot oils during the years 1997-1998, 1998-1999, and 1999-2000. the oils were analyzed ... | 2003 | 12502409 |
| [plant regeneration from agobacterium-mediated ctv-cp gene transformation of poncirus trifoliata raf]. | to lay a foundation for the resistant breeding, the anti-virus ctv-cp gene was transformed into the epicotyles mediated by agrobacterium tumefaciens in poncirus trifoliata. | 2001 | 12525114 |
| chloride absorption in salt-sensitive carrizo citrange and salt-tolerant cleopatra mandarin citrus rootstocks is linked to water use. | in this work, seedlings of two citrus rootstocks, the salt-tolerant cleopatra mandarin (citrus reshni hort. ex tan.) and the salt-sensitive carrizo citrange (citrus sinensis [l.] osb. x poncirus trifoliata [l.] raf.) were used to study the relationship between chloride and water uptake. the results indicated that net chloride uptake rates in both genotypes were alike and decreased linearly with the time of salinity exposure, although they were more rapidly reduced in the tolerant genotype. in ea ... | 2003 | 12554725 |
| the in vitro inhibitory effects of crude extracts of traditional chinese herbs on 3-hydroxy-3-methylglutaryl-coenzyme a reductase on vero cells. | cardiovascular disease is still the leading cause of death in western countries. epidemiological studies have shown that hypercholesterolemia is a major risk factor for coronary artery disease. clinical trials of lipid lowering therapy with 3-hydroxy-3-methylglutaryl coenzyme a (hmg co-a) reductase inhibitor have been shown to decrease coronary events and mortality. flavonoids are polyphenolic natural antioxidants occurring in natural products such as traditional chinese herbs, fruits and bevera ... | 2002 | 12568290 |
| sequence analysis of a 282-kilobase region surrounding the citrus tristeza virus resistance gene (ctv) locus in poncirus trifoliata l. raf. | citrus tristeza virus (ctv) is the major virus pathogen causing significant economic damage to citrus worldwide, and a single dominant gene, ctv, provides broad spectrum resistance to ctv in poncirus trifoliata l. raf. ctv was physically mapped to a 282-kb region using a p. trifoliata bacterial artificial chromosome library. this region was completely sequenced to about 8x coverage using a shotgun sequencing strategy and primer walking for gap closure. sequence analysis predicts 22 putative gene ... | 2003 | 12586873 |
| an in vitro system combined with an in-house quantitation assay for screening hepatitis c virus inhibitors. | hepatitis c virus (hcv) infection is a serious global health problem. interferon-alpha (ifn-alpha) and ribavirin have demonstrated efficacy in the treatment of hcv infection; however, these therapies display many side effects. to screen the anti-hcv compounds from plants, we established an in vitro model for inoculation of hcv by centrifugation-facilitated method. the hcv rna molecules were then quantitated by nested competitive reverse transcription-polymerase chain reaction (crt-pcr) using flu ... | 2003 | 12767467 |
| interaction between warfarin and the herbal product quilinggao. | to describe a patient with loss of anticoagulation control and bleeding after consumption of the combination herbal product quilinggao. | 2003 | 12773074 |
| identification and genomic distribution of gypsy like retrotransposons in citrus and poncirus. | transposable elements might be importantly involved in citrus genetic instability and genome evolution. the presence of gypsy like retrotransposons, their heterogeneity and genomic distribution in citrus and poncirus, have been investigated. eight clones containing part of the pol coding region of gypsy like retrotransposons have been isolated from a commercial variety of citrus clementina, one of the few sexual species in citrus. four of the eight clones might correspond to active elements give ... | 2003 | 12937896 |
| regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid. | a cdna clone coding for a gibberellin (ga) 20-oxidase ( ccga20ox1), an enzyme of ga biosynthesis, which when expressed in vitro catalyzed the conversion of ga(12) to ga(9) and of ga(53) to ga(20), was isolated from the citrus hybrid carrizo citrange (c itrus sinensis x poncirus trifoliata). transcripts of ccga20ox1 were abundant in the apex and leaves and much less abundant in internodes, nodes and roots. seedlings of carrizo citrange cultured under a 32 degrees c/27 degrees c (day/night) regime ... | 2003 | 14520571 |
| qtl analysis of citrus tristeza virus-citradia interaction. | citrus tristeza virus (ctv) has caused the death of millions of trees grafted on sour orange ( citrus aurantium). however, this rootstock is very well adapted to the mediterranean, semi-arid conditions. the aim of the present research is to genetically analyze the accumulation of ctv in a progeny derived from the cross between c. aurantium and poncirus trifoliata, both resistant to ctv isolate t-346. graft propagation of 104 hybrids was done on healthy sweet orange as a rootstock. three months l ... | 2004 | 14614564 |
| expressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance. | several studies have reported markers linked to a putative resistance gene from poncirus trifoliata ( ctv-r) located at linkage group 4 that confers resistance against one of the most important citrus pathogens, citrus tristeza virus (ctv). to be successful in both marker-assisted selection and transformation experiments, its accurate mapping is needed. several factors may affect its localization, among them two are considered here: the definition of resistance and the genetic background of prog ... | 2004 | 14624336 |
| effects of 15n application frequency on nitrogen uptake efficiency in citrus trees. | two irrigation systems were used to compare nitrogen uptake efficiency in citrus trees and to evaluate the no3- runoff in "navelina" orange trees [citrus sinensis (l.) osbeck] on carrizo citrange rootstock (citrus sinensis x poncirus trifoliata raf.). these were fertilized with 125 g n as labelled k15no3 and grown outdoors in containers filled with a sand-loamy soil. two groups of 3 trees received this n dose either in five equally split applications by a flooding irrigation system or in 66 appl ... | 2003 | 14717433 |
| poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells. | substances inducing apoptosis have shown efficacy in the treatment of cancers. poncirus trifoliata (l.) raf. (rutaceae) fruits (ptf) has been used for the treatment of various cancers among korean oriental medical doctors. | 2004 | 14734210 |
| nitrate improves growth in salt-stressed citrus seedlings through effects on photosynthetic activity and chloride accumulation. | we analyzed the effects of nitrate availability on growth of navelina (citrus sinensis (l.) osbeck) scions grafted on three citrus rootstocks differing in salt tolerance: carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.), citrus macrophylla wester and cleopatra mandarin (citrus reshni hort. ex tanaka). salt stress reduced total plant biomass by 27-38%, whereas potassium nitrate supplementation partially counteracted this effect by increasing dry matter and new leaf a ... | 2004 | 15234900 |
| introduction of a citrus blight-associated gene into carrizo citrange [citrus sinensis (l.) osbc. x poncirus trifoliata (l.) raf.] by agrobacterium-mediated transformation. | the protein p12 accumulates in leaves of trees with citrus blight (cb), a serious decline of unknown cause. the function of p12 is not known, but sequence analysis indicates it may be related to expansins. in studies to determine the function of p12, sense and antisense constructs were used to make transgenic carrizo citrange using an agrobacterium-mediated transformation system. homogeneous beta-glucuronidase+ (gus+) sense and antisense transgenic shoots were regenerated using kanamycin as a se ... | 2004 | 15248084 |
| passive cutaneous anaphylaxis-inhibitory activity of flavanones from citrus unshiu and poncirus trifoliata. | this study examined the passive cutaneous anaphylaxis-inhibitory activity of the flavanones isolated from the pericarp of citrus unshiu (family rutaceae) and the fruit of poncirus trifoliata (family rutaceae). naringenin, hesperetin and ponciretin potently inhibited ige-induced beta-hexosaminidase release from rbl-2h3 cells and the pca reaction. among the flavanones examined, naringenin was the most potent with an ic50 value for beta-hexosaminidase release from rbl-2h3 cells of 0.029 mm. intrape ... | 2005 | 15678369 |
| genetic analysis of citrus leafminer susceptibility. | damage caused by the citrus leafminer (clm), phyllocnistis citrella, is highly dependent on the citrus flushing pattern. chemical control is only required in young trees, both in nurseries and in newly established orchards. however, this situation is completely different in countries where the causal agent of citrus canker, the bacterium xanthomonas axonopodis pv. citri exists. clm infestation results in a higher incidence of citrus canker infection. among preventive control strategies that prov ... | 2005 | 15834698 |
| viral-like symptoms induced by the ectopic expression of the p23 gene of citrus tristeza virus are citrus specific and do not correlate with the pathogenicity of the virus strain. | ectopic expression of the p23 gene from a severe (t36) strain of citrus tristeza virus (ctv) induces viral-like symptoms in mexican lime. here, we report that expressing the same gene from a mild strain induced similar symptoms that correlated with accumulation of p23 protein irrespective of the source strain. ctv inoculation of transgenic limes showing ctv-like leaf symptoms and high p23 accumulation did not modify symptoms initially, with the virus titer being as in inoculated nontransgenic co ... | 2005 | 15915642 |
| [effects of arbuscular mycorrhiza on drought tolerance of poncirus trifoliata]. | this paper evaluated the effects of arbuscular mycorrhizal colonization on the growth and drought tolerance of poncirus trifoliata in a potted culture under natural water stress and resuming water supply. the results showed that the arbuscular mycorrhizal colonization by glomus mosseae 93 could significantly increase the plant height, stem diameter, and fresh weight of myocrrhizal plant. by the end of the experiment, the survival percentage of transplanted mycorrhizal plant was 7.1% higher than ... | 2005 | 15943357 |
| [effects of am fungi on drought tolerance of citrus grafting seedling trifoliate orange/cara cara]. | with pot experiment in a greenhouse, this paper studied the effects of arbuscular mycorrhizal (am) fungi glomus mosseae on the drought tolerance of citrus grafting seedling trifoliate orange/cara cara under natural water stress and rewatering. the results showed that inoculation with am fungi significantly increased the plant height, stem diameter, leaf area, and shoot length of test seedling. at the 4th day of rewatering, mycorrhizal plant had significantly higher root soluble protein content a ... | 2005 | 16110660 |
| identification of cold acclimated genes in leaves of citrus unshiu by mrna differential display. | citrus unshiu is freeze tolerant to -10 degrees c when fully acclimated after exposure to cold, nonfreezing temperatures. to gain an understanding of its cold tolerance mechanism, mrna differential display reverse transcriptase polymerase chain reaction (ddrt-pcr) and quantitative relative rt-pcr were used to study gene expression under a gradual cold-acclimation temperature regime. six up-regulated and two down regulated genes were identified based on their amino acid sequences. the identified ... | 2005 | 16125877 |
| expression analysis of a cold responsive transcript from trifoliate orange by real-time pcr and rt-pcr. | clta and cltb are two transcripts produced by the citrus clt gene. cltb is constitutively expressed, while clta is a low temperature responsive transcript expressed in temperatures below 10 degrees c during the acclimation process of some species of citrus. real-time pcr was used to study clta expression pattern in trifoliate orange during acclimation, gradual deacclimation and abrupt deacclimation. a standard curve of nine dilution series from 10 x 10(6) to 10 x 10(-3) fg was constructed, using ... | 2005 | 16151814 |
| effect of an aqueous extract of dried immature fruit of poncirus trifoliata (l.) raf. on intestinal transit in rodents with experimental gastrointestinal motility dysfunctions. | the effect of an aqueous extract of the dried immature fruit of poncirus trifoliata raf. (rutaceae) (pf-w) on gastrointestinal (gi) motor function was investigated by measuring the intestinal transit rate (itr) of evans blue in rats and mice with experimental gi motility dysfunctions (gmds). gmd was induced by appropriate surgery or by the administration of acetic acid, atropine, l-dopa, or morphine to the animals. the itr in these gmd animals was significantly retarded compared to normal animal ... | 2005 | 16169174 |
| prokinetic activity of an aqueous extract from dried immature fruit of poncirus trifoliata (l.) raf. | aqueous extracts from dried immature fruit of poncirus trifoliata raf. (rutaceae) (pf-w) are used as a traditional korean folk medicine for the treatment of digestive dysfunction. in the present study, pf-w exhibited no significant toxicity even at a dose of 5 g/kg when orally administered to mice. the effect of pf-w on gastrointestinal (gi) motor function was investigated by examining its effect on the serum concentration of orally administered ranitidine, a putative indicator of gi motility, i ... | 2005 | 16191468 |
| ectopic expression of an ft homolog from citrus confers an early flowering phenotype on trifoliate orange (poncirus trifoliata l. raf.). | citrus ft (cift) cdna, which promoted the transition from the vegetative to the reproductive phase in arabidopsis thaliana, when constitutively expressed was introduced into trifoliate orange (poncirus trifoliata l. raf.). the transgenic plants in which cift was expressed constitutively showed early flowering, fruiting, and characteristic morphological changes. they started to flower as early as 12 weeks after transfer to a greenhouse, whereas wild-type plants usually have a long juvenile period ... | 2005 | 16245161 |
| identification of qtls associated with citrus resistance to phytophthora gummosis. | citrus gummosis, caused by phytophthora spp., is an important citrus disease in brazil. almost all citrus rootstock varieties are susceptible to it to some degree, whereas resistance is present in poncirus trifoliata, a closely related species. the objective of this study was to detect qtls linked to citrus phytophthora gummosis resistance. eighty individuals of the f1 progeny, obtained by controlled crosses between sunki mandarin citrus sunki (susceptible) and poncirus trifoliata cv. rubidoux ( ... | 2006 | 16424605 |
| fatty acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae. | arbuscule-forming fungi in the order glomales form obligate endomycorrhizal associations with plants that make them difficult to quantify, and taxonomy of the group is only beginning to be objectively understood. fatty acid methyl ester (fame) profiles were analyzed to assess the diversity and quantity of fatty acids in 53 isolates of 24 glomalean species. spores and endomycorrhizal roots of sudan grass (sorghum sudanense) and the citrus rootstock carrizo citrange (poncirus trifoliata x citrus s ... | 1995 | 16534923 |
| anti-inflammatory effect of poncirus trifoliata fruit through inhibition of nf-kappab activation in mast cells. | mast cell-mediated allergic inflammation is involved in many diseases such as asthma, sinusitis, and rheumatoid arthritis. mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (tnf)-alpha and interleukin (il)-6 with immune regulatory properties. we investigated the effect of the fruits of poncirus trifoliata (l.) raf (rutaceae) (fpt) on expression of pro-inflammatory cytokines by activated human mast cell line, hmc-1. fpt dose dependently decre ... | 2006 | 16574371 |
| photosynthate partitioning in split-root citrus seedlings with mycorrhizal and nonmycorrhizal root systems. | photosynthate partitioning was examined in seedings of sour orange (citrus aurantium l.) and carrizo citrange (poncirus trifoliata [l.] raf. x c. sinensis [l.] osbeck) grown with split root systems inoculated on one side with vesicular-arbuscular mycorrhizal fungus (glomus intraradices schenck and smith). source-sink relations were studied without mitigating differences in mineral content or physiological age that can occur in separate plant comparisons, because phosphorus was evenly distributed ... | 1984 | 16663589 |
| de novo arginine biosynthesis in leaves of phosphorus-deficient citrus and poncirus species. | young, fully expanded leaves from 7-month-old p-deficient citrus rootstock seedlings had levels of nonprotein arginine that were 10- to 50-fold greater than those from p-sufficient control plants. arginine content of the protein fraction increased 2- to 4-fold in p-deficient leaves. total arginine content, which averaged 72 +/- 6 micromoles per gram dry weight of p-sufficient leaf tissue (mean +/- se, n = the four rootstocks) was 207, 308, 241, and 178 micromoles in p-deficient leaves from citru ... | 1984 | 16663918 |
| carbon cost of the fungal symbiont relative to net leaf p accumulation in a split-root va mycorrhizal symbiosis. | translocation of (14)c-photosynthates to mycorrhizal (+ +), half mycorrhizal (0+), and nonmycorrhizal (00) split-root systems was compared to p accumulation in leaves of the host plant. carrizo citrange seedlings (poncirus trifoliata [l.] raf. x citrus sinensis [l.] osbeck) were inoculated with the vesicular-arbuscular mycorrhizal fungus glomus intraradices schenck and smith. plants were exposed to (14) co(2) for 10 minutes and ambient air for 2 hours. three to 4% of recently labeled photosyntha ... | 1988 | 16665934 |
| potent protective effect of isoimperatorin against aflatoxin b1-inducible cytotoxicity in h4iie cells: bifunctional effects on glutathione s-transferase and cyp1a. | typically chemopreventive agents either induce phase ii detoxifying enzymes or inhibit the cytochrome p450 enzymes (cyps) that are required for the metabolism of carcinogens. in this study, we isolated a coumarin compound, isoimperatorin from poncirus trifoliata raf., and studied its protective effects against aflatoxin b1 (afb1)-induced cytotoxicity in h4iie cells. isoimperatorin (>0.3 microm) significantly inhibited the cytotoxic effect of afb1. cdnb [1-chloro-2,4-dinitrobenzene; glutathine s- ... | 2006 | 16829687 |
| refinement of the citrus tristeza virus resistance gene (ctv) positional map in poncirus trifoliata and generation of transgenic grapefruit (citrus paradisi) plant lines with candidate resistance genes in this region. | citrus tristeza virus (ctv) is a major pathogen of citrus. a single dominant gene ctv present in the trifoliate relative of citrus, poncirus trifoliata confers broad spectrum resistance against ctv. refinement of genetic maps has delimited this gene to a 121 kb region, comprising of ten candidate ctv resistance genes. the ten candidate genes were individually cloned in agrobacterium based binary vector and transformed into three ctv susceptible grapefruit varieties. two of the candidate r-genes, ... | 2006 | 16830176 |
| [arbuscular mycorrhizal fungal growth on citrus roots and its correlations with soil available phosphorus content and phosphatase activity]. | in 2004, a field investigation was made on the mycorrhizal infection rate, spore density, soil available phosphorus content, and soil phosphatase activity in the guoqing no. 1 (citrus unshiu )/trifoliate orange (poncirus trifoliata) and guoqing no. 4 (c. unshiu)/trifoliate orange (p. trifoliata) plantations, aimed to approach the correlations between the arbuscular mycorrhizal fungal growth on citrus roots and the available phosphorus content and phosphatase activity in rhizosphere soil. the res ... | 2006 | 16836102 |
| efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination. | the presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. in citrus, selection using the selectable marker gene nptii, that confers resistance to the antibiotic kanamycin, is in general very effective. an attractive alternative is offered by the mat system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system r/rs for removal of ma ... | 2007 | 16927091 |
| screening of potential chemopreventive compounds from poncirus trifoliata raf. | chemopreventive agents induce a battery of genes whose protein products can protect cells from chemical-induced carcinogenesis. in this study, we isolated three different coumarins compounds (1; poncimarin, 2; heraclenol 3'-methyl ester and 3; oxypeucedanin methanolate) from poncirus trifoliata raf., and studied whether these compounds increase glutathione s-transferase (gst) expression and activity in the h4iie cell-line (a rat hepatocyte cell line). cdnb (1-chloro-2,4-dinitrobenzene; gst subty ... | 2006 | 17020159 |
| reactive oxygen metabolism in mycorrhizal and non-mycorrhizal citrus (poncirus trifoliata) seedlings subjected to water stress. | the effect of the arbuscular mycorrhizal (am) fungus, glomus versiforme, on growth and reactive oxygen metabolism of trifoliate orange (poncirus trifoliata) seedlings was studied in potted plants under well-watered (ww) and water stressed (ws) conditions. water stress significantly decreased root colonization. shoot dry weight, plant height and stem diameter were higher in am than in non-am seedlings regardless of the water status. inoculation with g. versiforme increased root dry weight and lea ... | 2006 | 17032615 |
| analysis of 13000 unique citrus clusters associated with fruit quality, production and salinity tolerance. | improvement of citrus, the most economically important fruit crop in the world, is extremely slow and inherently costly because of the long-term nature of tree breeding and an unusual combination of reproductive characteristics. aside from disease resistance, major commercial traits in citrus are improved fruit quality, higher yield and tolerance to environmental stresses, especially salinity. | 2007 | 17254327 |
| molecular characterization of cevd strains that induce different phenotypes in gynura aurantiaca: structure-pathogenicity relationships. | two citrus exocortis viroid isolates (cevd-s and cevd-129) that induce severe and mild symptoms in gynura aurantiaca, respectively, have been characterized. they present nucleotide sequences in the pathogenicity motifs p(l), c and p(r) similar to those of "class a" and "class b". infectivity and symptom expression in g. aurantica and tomato were evaluated with a selection of sequence variants recovered from both isolates. as expected, the two variants selected from cevd-s induced severe symptoms ... | 2007 | 17393070 |
| differential display analysis of gene expression in etrog citron leaves infected by citrus viroid iii. | citrus are natural hosts of several viroids, which are plant pathogens composed exclusively of a non-protein-coding, small single-stranded circular rna that is able to replicate autonomously in susceptible hosts. they are responsible for symptoms such as stunting, leaf epinasty, and chlorosis. citrus viroid iii (cvd-iii) has been long regarded as a possible dwarfing agent of citrus grafted on trifoliate orange and its hybrids. to investigate molecular mechanisms involved in pathogenesis, the mes ... | 2007 | 17475349 |
| inhibition of colon cancer cell growth and antioxidant activity of bioactive compounds from poncirus trifoliata (l.) raf. | recently several plant derived natural compounds have been screened for their anticancer activity in order to identify putative compounds with novel structures or mechanism of action. in the present study, fruits of poncirus trifoliata were extracted with acetone and loaded onto silica gel column chromatography. the column was eluted with different solvents to obtain two bioactive compounds. the purity of compounds was analyzed by hplc and their structures were identified by 1h and 13c nmr exper ... | 2007 | 17512744 |
| leaf water relations and net gas exchange responses of salinized carrizo citrange seedlings during drought stress and recovery. | since salinity and drought stress can occur together, an assessment was made of their interacting effects on leaf water relations, osmotic adjustment and net gas exchange in seedlings of the relatively chloride-sensitive carrizo citrange, citrus sinensis x poncirus trifoliata. | 2007 | 17575285 |
| anti-inflammatory activity of 21(alpha, beta)-methylmelianodiols, novel compounds from poncirus trifoliata rafinesque. | the fruits of poncirus trifoliata (l.) are widely used in oriental medicine as a remedy for allergic inflammation. as a part of our program to screen medicinal plants for potential anti-inflammatory compounds, 21alpha-methylmelianodiol (21alpha-mmd) and 21beta-methylmelianodiol (21beta-mmd), which are two isomers of 21-methylmelianodiol isolated from the fruits of p. trifoliata for the first time, were found to inhibit nitric oxide (no) production in lipopolysaccharide (lps)-stimulated raw 264.7 ... | 2007 | 17662711 |
| growth inhibition and g1 cell cycle arrest mediated by 25-methoxyhispidol a, a novel triterpenoid, isolated from the fruit of poncirus trifoliata in human hepatocellular carcinoma cells. | poncirus trifoliata (rutaceae) extracts have been known to possess anti-allergic, anti-inflammatory and antiviral activities. however, other biological activities, especially, the anticancer potential of extracts of p. trifoliata or its constituents, have not been fully investigated yet. in this study, we have evaluated the antiproliferative effects of a novel triterpenoid, 25-methoxyhispidol a, isolated from the fruit of p. trifoliata against sk-hep-1 human hepatocellular carcinoma cells. flow ... | 2008 | 18219600 |
| antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus. | soil flooding constitutes a seasonal factor that negatively affects plant performance and crop yields. in this work, the relationship between oxidative damage and flooding sensitivity was addressed in three citrus genotypes with different abilities to tolerate waterlogging. we examined leaf visible damage, oxidative damage in terms of malondialdehyde (mda) concentration, leaf proline concentration, leaf and root ascorbate and glutathione contents and the antioxidant enzyme activities superoxide ... | 2008 | 18333999 |
| hesperidin, hesperidin methyl chalone and phellopterin from poncirus trifoliata (rutaceae) differentially regulate the expression of adhesion molecules in tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells. | the fruits of poncirus trifoliata (l.) are widely used in oriental medicine to treat allergic inflammation. recently, several active compounds including hesperidin, hesperidin methyl chalone and phellopterin from p. trifoliata (rutaceae) were isolated and characterized. the goal of this study was to investigate the differential effect of hesperidin, hesperidin methyl chalone and phellopterin derived from p. trifoliata (rutaceae) on the induction of intercellular adhesion molecule-1 (icam-1) and ... | 2008 | 18387509 |
| development and characterization of scar markers linked to the citrus tristeza virus resistance gene from poncirus trifoliata. | twelve new dominant randomly amplified polymorphic dna (rapd) fragments associated with a single dominant gene for resistance to citrus tristeza virus (ctv) were identified using bulked segregant analysis of an intergeneric backcross family. these and eight previously reported rapds were mapped in the resistance gene (ctv) region; the resulting localized linkage map spans about 32 cm, with nine close flanking markers within 2.5 cm of ctv. seven of 20 rapd fragments linked with the resistance gen ... | 1997 | 18464859 |
| gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress. | the effect of gibberellins (ga) on internode transcriptome was investigated in transgenic carrizo citrange (citrus sinensis x poncirus trifoliata) plants overexpressing endogenous ccga20ox1 (encoding a ga biosynthetic gene), and in non-transformed explants treated with ga(3), using a citrus cdna microarray. substantial modulation of gene expression was found in sense ccga20ox plants. extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those ... | 2008 | 18684239 |
| tetraploid citrus rootstocks are more tolerant to salt stress than diploid. | citrus trees are subject to several abiotic constraints such as salinity. providing new rootstocks more tolerant is thus a requirement. in this article, we investigated salt stress tolerance of three tetraploid rootstock genotypes when compared to their respective diploid rootstocks (poncirus trifoliata, carrizo citrange, cleopatra mandarin). plant growth, leaf fall and ion contents were investigated. at the end of the experiment, leaf fall was observed only for diploid poncirus trifoliata plant ... | 2008 | 18722990 |
| mapping the chromosomes of poncirus trifoliata raf. by bac-fish. | in spite of the importance of citrus in agriculture and recent progress in genetic mapping and cytogenetics of this group, chromosome mapping of citrus species is still limited to rdna probes. in order to obtain a better chromosome characterization of one species from this group, cma/dapi double staining followed by in situ hybridization using 45s rdna and 24 bacs (bac-fish) were used on poncirus trifoliata. the bacs used were obtained from a genomic library of this species and were selected by ... | 2008 | 18758171 |
| multiple citrus viroids in citrus from japan and their ability to produce exocortis-like symptoms in citron. | abstract sequential polyacrylamide gel electrophoresis analyses showed many viroid-like rnas in samples collected from citrus trees in japan. reverse transcription polymerase chain reaction and sequencing analyses of the amplified fragments verified that they were derived from variants of six citrus viroids, citrus exocortis viroid (cevd), citrus bent leaf viroid (cblvd) including cvd-i-lss (a distinct variant of cblvd), hop stunt viroid, citrus viroid iii, citrus viroid iv, and citrus viroid os ... | 2002 | 18943029 |
| interactions between citrus viroids affect symptom expression and field performance of clementine trees grafted on trifoliate orange. | abstract citrus exocortis viroid (cevd), citrus bent leaf viroid (cblvd), a noncachexia variant of hop stunt viroid (hsvd), citrus viroid iii (cvd-iii), and citrus viroid iv (cvd-iv) were co-inoculated as two-, three-, four-, and five-viroid mixtures to clementine trees grafted on trifoliate orange to evaluate their effect on symptom expression, tree growth, and fruit yield. most trees infected with cevd-containing viroid mixtures developed exocortis scaling symptoms, as did cevd alone, whereas ... | 2006 | 18943417 |
| protection of citrus rootstocks against phytophthora spp. with a hypovirulent isolate of phytophthora nicotianae. | abstract phytophthora root rot of citrus in florida is caused by phytophthora nicotianae and p. palmivora. a naturally occurring isolate of p. nicotianae (pn117) was characterized as hypovirulent on citrus roots. pn117 infected and colonized fibrous roots, but caused significantly less disease than the virulent isolates p. nicotianae pn198 and p. palmivora pp99. coincident inoculation of rootstock seedlings of cleopatra mandarin (citrus reticulata) or swingle citrumelo (c. paradisi x poncirus tr ... | 2007 | 18943635 |
| histological comparison of fibrous root infection of disease-tolerant and susceptible citrus hosts by phytophthora nicotianae and p. palmivora. | abstract phytophthora nicotianae and p. palmivora infect and cause rot of fibrous roots of susceptible and tolerant citrus rootstocks in florida orchards. the infection and colonization by the two phytophthora spp. of a susceptible citrus host, sour orange (citrus aurantium), and a tolerant host, trifoliate orange (poncirus trifoliata), were compared using light and electron microscopy. penetration by both phytophthora spp. occurred within 1 h after inoculation, regardless of the host species. n ... | 1998 | 18944916 |
| high-efficiency agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration. | an improved method for the agrobacterium infiltration of epicotyl segments of 'pineapple' sweet orange [citrus sinensis (l.) osbeck] and 'swingle' citrumelo [citrus paradisi macf. x poncirus trifoliata (l.) raf.] was developed in order to increase transformation frequency. sonication-assisted agrobacterium-mediated transformation (saat), vacuum infiltration, and a combination of the two procedures were compared with conventional agrobacterium-mediated inoculation method ('dipping' method). it wa ... | 2009 | 19048258 |
| characterization of gibberellin 20-oxidases in the citrus hybrid carrizo citrange. | ccga20ox1 is a gene encoding a ga 20-oxidase, a gibberellin (ga) biosynthetic enzyme, previously isolated from the citrus hybrid carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.). southern blot analysis of genomic dna of carrizo citrange with ccga20ox1 suggested the presence in the hybrid of another gene encoding another ga 20-oxidase. a cdna clone from this new gene (ccga20ox2) was isolated using rna from the other parent c. sinensis. ccga20ox2 encoded a protein of ... | 2009 | 19203975 |
| observations on the mode of parasitism and histopathology of meloidodera floridensis and verutus volvingentis (heteroderidae). | some aspects of the host-parasite relationships of two heteroderid nematodes are described. meloidodera floridensis induced formation of single uninucleate giant cells in the stelar parenchyma tissue of sand pine (pinus clausa) roots. wrinkling and yellowing of the cuticle were associated with maturation of the adult female (cystoid stage). the mode of parasitism of different life stages of verutus volvingentis on buttonweed (diodia virginiana) is described. the nematode caused extensive necrosi ... | 1984 | 19294021 |
| influence of the sting nematode, belonolaimus longicaudalus, on young citrus trees. | the sting nematode, belonolaimus longicaudatus, was associated with poor growth of citrus in a central florida nursery. foliage of trees was sparse and chlorotic. affected rootstocks included changsha and cleopatra mandarin orange; flying dragon, rubidoux, and jacobsen trifoliate orange; macrophylla and milam lemon; palestine sweet lime; sour orange; and the hybrids - carrizo, morton, and rusk citrange and swingle citrumelo. root symptoms included apical swelling, development of swollen terminal ... | 1985 | 19294118 |
| variation in radopholus citrophilus population densities in the citrus rootstock carrizo citrange. | seedlings of the hybrid citrus rootstock, carrizo citrange (citrus sinensis x poncirus trifoliata) do not uniformly limit development of the citrus burrowing nematode, radopholus citrophilus. variation in nematode population densities in roots of seedlings germinating from the same seed suggests that factors responsible for nematode incompatibility are not functional or are not inherited uniformly among progeny. seeds which produced a single seedling were more likely to produce plants which supp ... | 1986 | 19294135 |
| reaction of citrus rootstocks to meloidogyne javanica. | the response of citrus spp. and related rootstocks to a population of meloidogyne javanica was evaluated in a screenhouse experiment. palestine and rangpur lime, rough lemon, sour orange, sexton and thentriton tangelo, and volkamer lemon were not infected by m. javanica. galls and tip swellings were observed on the roots of poncirus triloliata and troyer citrange. there was no evidence of nematode development. symptoms induced by the nematode were stelar division, syncytia formation in the vascu ... | 1978 | 19305835 |
| differences in seasonal expression of flowering genes between deciduous trifoliate orange and evergreen satsuma mandarin. | to determine differences in seasonal flowering between evergreen and deciduous woody perennials, endogenous expression of flowering-related genes was investigated in satsuma mandarin (citrus unshiu marc.) and its close relative, trifoliate orange (poncirus trifoliata (l.) raf.), which are evergreen and deciduous, respectively, and show different seasonal flowering characteristics. in satsuma mandarin, in which floral induction is triggered by low temperatures during fall and winter, mrna levels ... | 2009 | 19364704 |
| protective effects of neohesperidin and poncirin isolated from the fruits of poncirus trifoliata on potential gastric disease. | the effects of poncirus trifoliata (p. trifoliata) (ponciri fructus, pf) extract and its constituents such as neohesperidin and poncirin on gastritis in rats and human gastric cancer cells were investigated. the pf 70% ethanol extracts (1 g) showed approximately 11.38% of acid-neutralizing capacities and cytotoxicity (ic50=85.39 microg/ml) against human ags gastric cancer cells. in addition, neohesperidin exhibited antioxidant activity (ic50=22.31 microg/ml) in the 1,1-diphenyl-2-picryldydrazyl ... | 2009 | 19367677 |
| effect of citrus hosts on the generation, maintenance and evolutionary fate of genetic variability of citrus exocortis viroid. | citrus exocortis viroid (cevd) populations are composed of closely related haplotypes whose frequencies in the population result from the equilibrium between mutation, selection and genetic drift. the genetic diversity of cevd populations infecting different citrus hosts was studied by comparing populations recovered from infected trifoliate orange and sour orange seedling trees after 10 years of evolution, with the ancestral population maintained for the same period in the original host, etrog ... | 2009 | 19403756 |
| inhibitory effect of poncirus trifoliate on acetylcholinesterase and attenuating activity against trimethyltin-induced learning and memory impairment. | various native korean plants were screened to find an effective acetylcholinesterase (ache) inhibitor for the treatment of alzheimer's disease (ad). among these plants, the ethanol extract of poncirus trifoliate was selected for isolating the ache inhibitor because it exhibited the highest inhibitory activity (47.31%). to separate the active compound from poncirus trifoliate, solvent partition, open column chromatography, thin-layer chromatography (tlc), and high-performance liquid chromatograph ... | 2009 | 19420715 |
| long-term administration of an aqueous extract of dried, immature fruit of poncirus trifoliata (l.) raf. suppresses body weight gain in rats. | the purpose of the present study was to examine the effects of daily administration of an aqueous extract of the dried, immature fruit of poncirus trifoliata raf. (rutaceae) (pf-w) on body weight in rats. | 2009 | 19703543 |
| hexane extract of poncirus trifoliata (l.) raf. stimulates the motility of rat distal colon. | poncirus trifoliata (l.) raf. (rutaceae, pt) has been commonly used for treating gastrointestinal (gi) disorders in korean traditional medicine, but its pharmacological roles in the regulation of colonic motility have not been clarified. this study investigated the regulatory effects of pt on the colonic motility. | 2010 | 19963058 |
| [effects of arbuscular mycorrhizal fungi on different iron species in poncirus trifoliata rhizospheric soil]. | we studied the effects of arbuscular mycorrhizal (am) fungus (glomus versiforme) on iron species at different trifoliate orange [poncirus trifoliata (l.) raf.] rhizospheric soil with pot culture. | 2009 | 20069881 |
| [effects of arbuscular mycorrhizal fungi on growth and iron uptake of poncirus trifoliata under different ph]. | we studied the effects of arbuscular mycorrhizal fungus ( glomus versiforme) on growth and iron uptake of trifoliate orange [poncirus trifoliata (l.) raf.] at different ph levels of nutrient solution. | 2009 | 20069885 |
| isolates of citrus tristeza virus that overcome poncirus trifoliata resistance comprise a novel strain. | the economically important rootstock species poncirus trifoliata is resistant to most isolates of citrus tristeza virus (ctv), but not to members of the ctv resistance-breaking (rb) strain presently found in new zealand. in this study, five known and suspected rb isolates were separated from field mixtures, and their genomes were sequenced in full. it was found that the rb isolates are members of a single phylogenetically distinct clade with an average of 90.3% genomic nucleotide sequence identi ... | 2010 | 20352212 |
| the evolution of cma bands in citrus and related genera. | most species of citrus and related genera display a similar karyotype with 2n = 18 and a variable number of terminal heterochromatic blocks positively stained with chromomycin a(3) (cma(+) bands). some of these blocks are 45s rdna sites, whereas others may correspond to the main gc-rich satellite dna found in several citrus species. in the present work, the distribution of the 45s rdna and the main satellite dna isolated from c. sinensis (cssat) were investigated by in situ hybridization in seve ... | 2010 | 20490650 |
| ptsvp, an svp homolog from trifoliate orange (poncirus trifoliata l. raf.), shows seasonal periodicity of meristem determination and affects flower development in transgenic arabidopsis and tobacco plants. | a mads-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (poncirus trifoliata l. raf.). this gene is highly homologous with arabidopsis short vegetative phase (svp). based on real-time pcr and in situ hybridization during bud differentiation, ptsvp was expressed intensively in dormant tissue and vegetative meristems. ptsvp transcripts were detected in apical meristems before floral transition, then down-re ... | 2010 | 20602150 |
| anti-human immunodeficiency virus-1 constituents of the bark of poncirus trifoliata. | a total of 36 compounds (1-36) were obtained from the stem bark of poncirus trifoliata including three new prenylated flavonoids, (-)-5,4'-dihydroxy-7,8-[(3'',4''-cis-dihydroxy-3'',4''-dihydro)-2'',2''-dimethylpyrano]-flavone (1), (-)-5,4'-dihydroxy-7,8-[(3''-hydroxy-4''-one)-2'',2''-dimethylpyrano]-flavone (2), and (-)-5,4'-dihydroxy-7,8-[(cis-3''-hydroxy-4''-ethoxy-3'',4''-dihydro)-2'',2''-dimethylpyrano]-flavone (3). the new structures were elucidated by means of spectroscopic methods. compou ... | 2010 | 20606349 |
| peroxyl radical scavenging capacity of extracts and isolated components from selected medicinal plants. | we determined the ability of extracts and active components isolated from nine medicinal plants, poncirus trifoliata, astragalus membranaceus, magnolia obovata, salvia miltiorrhiza, angelica dahurica, cornus officinalis, cnidium officinale, pueraria lobata and ostericum koreanum, to neutralize peroxyl radicals using the total oxyradical scavenging capacity (tosc) assay. peroxyl radicals were generated from thermal homolysis of 2,2'-azobis(2-methylpropionamidine) dihydrochloride, which oxidize al ... | 2010 | 20607491 |
| an aqueous extract of poncirus fructus activates the prokinetic activity of 5-ht receptor subtype 4 without herg interaction. | poncirus fructus (pf)--also known as the dried, immature fruit of poncirus trifoliata (l.) raf. (rutaceae)--is a natural substance that has long been used for various gastrointestinal disorders in eastern asia. an aqueous extract of pf (pf-w) has particularly potent gastroprokinetic effects, but its molecular mechanism was not well understood. identification of the underlying prokinetic mechanism of pf-w was pursued in the present study. | 2010 | 20736054 |
| overexpression of ptrabf gene, a bzip transcription factor isolated from poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ros and modulating expression of stress-responsive genes. | drought is one of the major abiotic stresses affecting plant growth, development and crop productivity. aba responsive element binding factor (abf) plays an important role in stress responses via regulating the expression of stress-responsive genes. | 2010 | 20973995 |
| [effects of zinc- and iron deficiency on physiological indices, mineral contents, and leaf ultrastructure of poncirus trifoliata]. | by using hydroponics, this paper studied the physiological responses of trifoliate orange (poncirus trifoliata) seedlings to the deficiency of zinc (0 micromol x l(-1) zn2+) and/or iron (0 micromol x l(-1) fe-edta). the deficiency of both zn and fe decreased the plant biomass and root viability, and increased the leaf-and root sod activity significantly. zinc deficiency increased the leaf-and root pod significantly, while fe deficiency had an adverse effect. the root cat activity increased signi ... | 2010 | 21043103 |
| phylogenetic and evolutionary analysis of nbs-encoding genes in rutaceae fruit crops. | the nucleotide-binding site leucine-rich repeat (nbs-lrr) genes are the largest class of disease resistance genes in plants. however, our understanding of the evolution of nbs-lrr genes in rutaceae fruit crops is rather limited. we report an evolutionary study of 103 nbs-encoding genes isolated from poncirus trifoliata (trifoliate orange), citrus reticulata (tangerine) and their f(1) progeny. in all, 58 of the sequences contained a continuous open reading frame. phylogenetic analysis classified ... | 2010 | 21153735 |
| somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the mediterranean citrus industry. | the prevalence of sour orange rootstock in the southern and eastern part of the mediterranean basin is presently threatened by the spread of citrus tristeza virus (ctv) and its main vector toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. the search for alternative ctv-resistant rootstocks that also withstand the other constraints is now considered an urgent priority for a sustainable citrus industry in the area. complementary progenitors can be fou ... | 2011 | 21225429 |