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the sub/supra-optimal temperature-induced inhibition of photosynthesis and oxidative damage in cucumber leaves are alleviated by grafting onto figleaf gourd/luffa rootstocks.shoot-root communication is involved in plant stress responses, but its mechanism is largely unknown. to determine the role of roots in stress tolerance, cucumber (cucumis sativus) shoots from plants with roots of their own or with figleaf gourd (cucurbita ficifolia, a chilling-tolerant species) or luffa (luffa cylindrica (l.) m. roem., a heat-tolerant species) rootstocks were exposed to low (18/13°c), optimal (27/22°c) and high (36/31°c) temperatures, respectively. grafting onto figleaf gourd a ...201424735050
effect of cucurbita ficifolia and probiotic yogurt consumption on blood glucose, lipid profile, and inflammatory marker in type 2 diabetes.control of blood sugar, hypertension, and dyslipidemia are key factors in diabetes management. cucurbita ficifolia (pumpkin) is a vegetable which has been used traditionally as a remedy for diabetes in iran. in addition, consumption of probiotics may have beneficial effects on people with type 2 diabetes. the aim of this study was an investigation of the effects of c. ficifolia and probiotic yogurt consumption alone or at the same time on blood glucose and serum lipids in diabetic patients.201626955460
grafting of cucumis sativus onto cucurbita ficifolia leads to improved plant growth, increased light utilization and reduced accumulation of reactive oxygen species in chilled plants.the effects of chilling at 14 and 7 degrees c on plant growth, co(2) assimilation, light allocation, photosynthetic electron flux and antioxidant metabolism were examined in cucumber (cucumis sativus l. cv. jinyan no. 4, cs) plants with figleaf gourd (cucurbita ficifolia bouché, cf) and cucumber as rootstocks, respectively. growth inhibition by chilling at 7 degrees c was characterized by irreversible inhibition of co(2) assimilation in grafted plants with cucumber as rootstock and scion (cs/cs) ...200919521746
overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic arabidopsis thaliana.polyamines play pivotal roles in plant defense to environmental stresses. however, stress tolerance of genetically engineered plants for polyamine biosynthesis has been little examined so far. we cloned spermidine synthase cdna from cucurbita ficifolia and the gene was introduced to arabidopsis thaliana under the control of the cauliflower mosaic virus 35s promoter. the transgene was stably integrated and actively transcribed in the transgenic plants. as compared with the wild-type plants, the t ...200415215506
an attractive way of egg white protein by-product use for producing of novel anti-hypertensive peptides.the aim of this study was to (i) examine how enzymatic hydrolysis with a non-commercially available proteinase of fig-leaf gourd fruit (cucurbita ficifolia) increased the use value of egg white protein preparations, generated as byproducts in the industrial process of lysozyme and cystatin isolation from egg white, and (ii) evaluate the inhibition of angiotensin i-converting enzyme (ace) by the obtained hydrolysates. purification procedures including membrane filtration, gel filtration chromatog ...201424423562
cytotoxic activity of four mexican medicinal plants.ibervillea sonorae greene, cucurbita ficifolia bouché, tagetes lucida cav and justicia spicigera scheltdd are mexican native plants used in the treatment of different illnesses. the ethanolic extract of j. spicigera and t. lucida as well as aqueous extracts from i. sonorae, c. ficifolia, t. lucida and j. spicigera were investigated using sulforhodamine b assay. these extracts were assessed using two cell line: t47d (human breast cancer) and hela (human cervix cancer). colchicine was used as the ...200922128430
d-chiro-inositol found in cucurbita ficifolia (cucurbitaceae) fruit extracts plays the hypoglycaemic role in streptozocin-diabetic rats.cucurbita ficifolia is commonly used as an antihyperglycaemic agent in asia. however, the mechanism of its action is unknown. chemically synthesized d-chiro-inositol (d-ci), a component of an insulin mediator, has been demonstrated to have antihyperglycaemic effects in rats. in this study, we found that c. ficifolia contained fairly high levels of d-ci, thus, c. ficifolia may be a natural source of d-ci for reducing blood glucose concentrations in diabetics. we evaluated c. ficifolia fruit extra ...200617132216
in vitro biocompatibility and proliferative effects of polar and non-polar extracts of cucurbita ficifolia on human mesenchymal stem cells.cucurbita ficifolia (c. ficifolia) has been traditionally known for its medicinal properties as an antioxidant, anti-diabetic and anti-inflammatory agent. however, there has been an enduring attention towards the identification of unique method, to isolate the natural components for therapeutic applications. our study focuses on different polar and non-polar solvents (methanol, hexane and chloroform) to extract the bioactive components from c. ficifolia (pumpkin) and to study the biocompatibilit ...201728231542
[fusarium species of the quebrada de lozano, jujuy, argentina].a soil sampling was made in 50 cultivated places with horticultural and floral species where there were fusariosis records. for the isolation bengal rose agar, peptone dichloran chloramphenicol agar and cayote dichloran chloramphenicol agar were used, being this last one, the more convenient. the isolates were identified as f. acuminatum, f. avenaceum, f. graminearum, f. merismoides, f. oxysporum, f. poae, f. proliferatum, f. semitectum, f. solani, f. subglutinans and f. sporotrichioides. it was ...200516178470
mexican plants with hypoglycaemic effect used in the treatment of diabetes.diabetes mellitus is a syndrome which affects more and more people in all countries over the world. in méxico, it is commonly treated with herbal extracts. such treatment may be of considerable benefit especially during the early stages of the illness. in this review, we discuss species commonly used in méxico in the treatment of diabetes. a total of 306 species have records of a popular use in the treatment of this syndrome in méxico. seven of these species--cecropia obtusifolia bertol. (cecrop ...200515964161
evaluation of the hypoglycemic effect of cucurbita ficifolia bouché (cucurbitaceae) in different experimental models.acute hypoglycemic effects of freeze-dried juice of cucurbita ficifolia bouché (cucurbitaceae) fruits were studied in healthy and alloxan-diabetic mice. c. ficifolia fruit administered by intraperitoneal route produced, in a dose-dependent manner, a significant decrease of the glycemia in healthy mice. although oral route of c. ficifolia fruit juice also caused significant reductions of blood glucose levels in healthy mice, the effect was minor. the juice administered by intraperitoneal route sh ...200212241994
anti-hyperglycemic effect of some edible plants.the anti-hyperglycemic effect of 12 edible plants was studied on 27 healthy rabbits, submitted weekly to subcutaneous glucose tolerance tests after gastric administration of water, tolbutamide or a traditional preparation of the plant. tolbutamide, cucurbita ficifolia, phaseolus vulgaris, opuntia streptacantha, spinacea oleracea, cucumis sativus and cuminum cyminum decrease significantly the area under the glucose tolerance curve and the hyperglycemic peak. brassica oleracea var. botrytis, alliu ...19958569244
cucurbita ficifolia (cucurbitaceae) modulates inflammatory cytokines and ifn-γ in obese mice.this study investigated the effect of aqueous extract of cucurbita ficifolia bouché on systemic chronic inflammation in an obesity model induced by monosodium glutamate (msg) via modulating the expression of adipokines (tnf-α, il-6, resistin, and adiponectin) and immune-regulatory cytokines (ifn-γ and il-10). cucurbita ficifolia extract was administered daily by gavage to lean and msg-obese mice for 30 days. at the end of treatment, cytokine mrna expression in adipose tissue was determined by re ...201727918843
chemical characterization of a hypoglycemic extract from cucurbita ficifolia bouche that induces liver glycogen accumulation in diabetic mice.the aqueous extract of cucurbita ficifolia (c. ficifolia) fruit has demonstrated hypoglycemic effect, which may be attributed to some components in the extract. however, the major secondary metabolites in this fruit have not yet been identified and little is known about its extra-pancreatic action, in particular, on liver carbohydrate metabolism. therefore, in addition to the isolation and structural elucidation of the principal components in the aqueous extract of c. ficifolia, the aim of this ...201728480434
immunocytochemical localization of lectin in cotyledons of developing seedlings of cucurbita ficifolia. 19947976049
developmental differences in acid phosphatase and agglutination activity in roots of cucurbita ficifolia seedlings. purification and some properties of lectin. 19947976045
aspartic proteinase from the seeds of figleaf gourd (cucurbita ficifolia)*. 19947976038
lectins from squash (cucurbita ficifolia) seedlings. 19938372529
translocation of structural p proteins in the phloem.phloem-specific proteins (p proteins) are particularly useful markers to investigate long-distance trafficking of macromolecules in plants. in this study, genus-specific molecular probes were used in combination with intergeneric grafts to reveal the presence of a pool of translocatable p protein subunits. immunoblot analyses demonstrated that cucurbita spp p proteins pp1 and pp2 are translocated from cucurbita maxima stocks and accumulate in cucumis sativus scions. cucurbita maxima or cucurbita ...19999878637
gating of aquaporins by low temperature in roots of chilling-sensitive cucumber and chilling-tolerant figleaf gourd.effects of low temperature (8 degrees c) on the hydraulic conductivity of young roots of a chilling-sensitive (cucumber, cucumis sativus l.) and a chilling-resistant (figleaf gourd, cucurbita ficifolia bouche) crop have been measured at the levels of whole root systems (root hydraulic conductivity, lp(r)) and of individual cortical cells (cell hydraulic conductivity, lp). exposure of roots to low temperature (lrt) for up to 6 d caused a stronger suberization of the endodermis in cucumber compare ...200515734792
chill-induced decrease in capacity of rubp carboxylation and associated h2o2 accumulation in cucumber leaves are alleviated by grafting onto figleaf gourd.chilling results in a significant decrease in rubisco content and increased generation of reactive oxygen species (ros) in cucumber (cucumis sativus), a chilling-sensitive species. the role of roots in the regulation of the tolerance is unknown. here, cucumber plants grafted onto figleaf gourd (cucurbita ficifolia), a chilling-tolerant species were used to study the role of roots in the regulation of shoot functioning and the associated root-to-shoot communication.200717761689
cucurbita ficifolia bouché increases insulin secretion in rinm5f cells through an influx of ca(2+) from the endoplasmic reticulum.cucurbita ficifolia bouché(c. ficifolia) is a plant used in mexican traditional medicine to control type 2 diabetes (t2d). the hypoglycemic effect of the fruit of c. ficifolia has been demonstrated in different experimental models and in t2d patients. it has been proposed that d-chiro-inositol (dci) is the active compound of the fruit. additionally, it has been reported that c. ficifolia increases the mrna expression of insulin and kir 6.2 (a component of the atp-sensitive potassium (k(+)atp) ch ...201627174079
selective trans-cinnamic acid uptake impairs [ca2+]cyt homeostasis and growth in cucumis sativus l.to obtain insight into interspecies interactions mediated by allelochemicals, the response of cucumber (cucumis sativus l. cv jinyan no.4) and figleaf gourd (cucurbita ficifolia bouché) seedlings to trans-cinnamic acid (ca) (1) was investigated. while trans-ca is an autotoxin in cucumber root exudates, figleaf gourd is resistant to it. cucumber, however, had a high rate of trans-ca uptake by the roots, leading to reduced root growth. the trans-ca treatment also resulted in an intracellular relea ...200920063205
the evaluation of dipeptidyl peptidase (dpp)-iv, α-glucosidase and angiotensin converting enzyme (ace) inhibitory activities of whey proteins hydrolyzed with serine protease isolated from asian pumpkin (cucurbita ficifolia).in the present study, whey protein concentrate (wpc-80) and β-lactoglobulin were hydrolyzed with a noncommercial serine protease isolated from asian pumpkin (cucurbita ficifolia). hydrolysates were further fractionated by ultrafiltration using membranes with cut-offs equal 3 and 10 kda. peptide fractions of molecular weight lower than 3 and 3-10 kda were further subjected to the rp-hplc. separated preparations were investigated for their potential as the natural inhibitors of dipeptidyl peptidas ...201425364320
egg-yolk protein by-product as a source of ace-inhibitory peptides obtained with using unconventional proteinase from asian pumpkin (cucurbita ficifolia).in the present study angiotensin i-converting enzyme (ace) inhibitory peptides were isolated from egg-yolk protein preparation (yp). enzymatic hydrolysis conducted using unconventional enzyme from cucurbita ficifolia (dose: 1000 u/mg of hydrolyzed yp (e/s (w/w)=1:7.52)) was employed to obtain protein hydrolysates. the 4-h hydrolysate exhibited a significant (ic₅₀=482.5 μg/ml) ace inhibitory activity. moreover, hydrolysate showed no cytotoxic activity on human and animal cell lines which makes it ...201425138009
hydrolysis with cucurbita ficifolia serine protease reduces antigenic response to bovine whey protein concentrate and αs-casein.in the present study the effect of hydrolysis with non-commercial cucurbita ficifolia serine protease on a reduction of the ige and igg binding capacity of whey protein concentrate and αs-casein was investigated. the intensity of the protein degradation was analyzed by the degree of hydrolysis, the free amino groups content and rp-hplc. the ability to bind the antibodies by native proteins and their hydrolysates was determined using a competitive elisa test. deep hydrolysis contributed to a sign ...201526036686
cucurbita ficifolia bouché (cucurbitaceae) and d-chiro-inositol modulate the redox state and inflammation in 3t3-l1 adipocytes.cucurbita ficifolia (characterised by its d chiro inositol (dci) content) and of synthetic dci on the redox state, mrna expression and secretions of proinflammatory cytokines. additionally, we evaluated the insulin-mimetic action of both treatments by assessing protein kinase b (pkb) activation in 3t3-l1 adipocytes.201324028624
effect of an aqueous extract of cucurbita ficifolia bouché on the glutathione redox cycle in mice with stz-induced diabetes.cucurbita ficifolia is used in mexican traditional medicine as an anti-diabetic and anti-inflammatory agent and its actions can be mediated by antioxidant mechanisms. disturbance in the homeostasis of glutathione has been implicated in the etiology and progression of diabetes mellitus and its complications.201222960550
[effects of root exudates from cucumber and squash on fusarium wilt occurrence].with the root exudates of two cucumber varieties jinyan 4 (susceptible variety) and jinchun 4 (resistant variety) and of black seed squash variety as test materials, this paper studied their effects and action mechanisms on the occurrence of fusarium wilt on jinyan 4. the results showed that the occurrence of fusarium wilt was earlier when treated with the root exudates of jinyan 4, and the infection rate was significantly higher at 15 days after inoculation, but nearly the same as the control a ...200717552193
antioxidant and anti-inflammatory effects of a hypoglycemic fraction from cucurbita ficifolia bouché in streptozotocin-induced diabetes mice.type 2 diabetes is characterized by oxidative stress and a chronic low-grade inflammatory state, which also play roles in the pathogenesis of this disease and the accompanying vascular complications by increasing the production of free radicals and pro-inflammatory cytokines. cucurbita ficifolia bouché (c. ficifolia) is an edible mexican plant whose hypoglycemic activity has been demonstrated in several experimental and clinical conditions. recently, d-chiro-inositol has been proposed as the com ...201222298451
[effects of grafting on root polyamine metabolism of cucumber seedlings under copper stress].by the method of hydroponic culture, and taking cucurbita ficifolia b. as rootstock, this paper studied the effects of grafting on the root polyamine metabolism of cucumber seedlings under copper stress. the results showed that under copper stress, the root activities of cucumber seedlings were inhibited, and electrolyte leakage increased, with these changes being significantly lower for grafted than for ungrafted cucumber seedlings. in addition, the contents of free spermidine and spermine, and ...201021043115
light-induced transpiration alters cell water relations in figleaf gourd (cucurbita ficifolia) seedlings exposed to low root temperatures.water relation parameters including elastic modulus (epsilon), half-times of water exchange (t(w)(1/2)), hydraulic conductivity and turgor pressure (p) were measured in individual root cortical and cotyledon midrib cells in intact figleaf gourd (cucurbita ficifolia) seedlings, using a cell pressure probe. transpiration rates (e) of cotyledons were also measured using a steady-state porometer. the seedlings were exposed to low ambient (approximately 10 micromol m(-2) s(-1)) or high supplemental i ...200818346079
physiological basis of different allelopathic reactions of cucumber and figleaf gourd plants to cinnamic acid.to provide an insight into the mechanism of interspecific interactions mediated by allelochemicals, cucumber and figleaf gourd seedlings were compared on their response to cinnamic acid, an autotoxin from root exudates of cucumber. reactive oxygen species metabolism and plasma membrane h(+)-atpase activity were examined in roots upon exposure to cinnamic acid. this exposure resulted in significant increases in activities of nadph oxidase, superoxide dismutase, guaiacol peroxidase, and catalase, ...200717965143
differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in figleaf gourd and cucumber roots.previous studies show that low temperature strongly induces suberin layers in the roots of chilling-sensitive cucumber plants, while in contrast, low temperature produces a much weaker induction of suberin layers in the roots of the chilling-tolerant figleaf gourd [s.h. lee, g.c. chung, s. steudle, gating of aquaporins by low temperature in roots of chilling-sensitive cucumber and -tolerant figleaf gourd, j. exp. bot. 56 (2005) 985-995; s.h. lee, g.c. chung, e. steudle, low temperature and mecha ...200515823569
application of asian pumpkin (cucurbita ficifolia) serine proteinase for production of biologically active peptides from casein.the main objective of this study was to determine potential application of a serine proteinase derived from asian pumpkin for obtaining biologically active peptides from casein. the course of casein hydrolysis by three doses of the enzyme (50, 150, 300 u/mg of protein) was monitored for 24 hours by the determinations of: hydrolysis degree dh (%), free amino group content (μmole gly/g), rp hplc peptide profiles and by polyacrylamide gel electrophoresis. in all hydrolyzates analyzed antioxidant ac ...201323520577
hypoglycemic action of cucurbita ficifolia on type 2 diabetic patients with moderately high blood glucose levels.ten type 2 diabetic patients with moderately hyperglycemia, received a raw extract of cucurbita ficifolia or potable water in a single dose of 4 ml/kg body weight, in two different sessions at least separated by 1 week. blood glucose levels were followed in both parts of the study by a period of 5 h. the patients had stopped their pharmacologic medication 24 h prior to each part of the study. as expected, no significant changes on blood glucose were observed after giving the vehicle; however, th ...200111483384
[effects of nacl stress on cation contents in seedlings of two pumpkin varieties].effects of different concentration nacl stress on cation contents of pumpkin cultivars (cucurbita ficifolia and cucurbita moschata) usually used as rootstocks of cucumber were studied. the results showed that, in both cultivars treated with 150, 300 and 500 mmol/l nacl for 7 d, increasing salinity caused in increasing retardation of shoot and root growth, and reduction of shoot relative water content (table 1). with increasing nacl concentration, na(+) content increased while k(+) content decrea ...200616477137
chloroplast dna diversity among wild and cultivated members of cucurbita (cucurbitaceae).cladistic analysis of 86 chloroplast dna restriction-site mutations among 30 samples representing 15 species of cucurbita indicates that annual species of the genus are derived from perennials. the malabar gourd, c. ficifolia, is placed as a basal, sister taxon relative to other domesticated species and allied wild-types. the pattern of variation supports three species groups as monophyletic: (1) c. fraterna, c. pepo, and c. texana, (2) c. lundelliana, c. martinezii, c. mixta, c. moschata and c. ...199224201487
serine proteinase from cucurbita ficifolia seeds.a new serine proteinase was isolated from cucurbita ficifolia seeds by the purification procedure, which includes: extraction, salting out with ammonium sulphate, chromatography on cm-cellulose. sephacryl s-300 gel filtration and h.p.l.c. on deae-2sw tsk column. the enzyme was homogeneous both in native and sds page. three independent methods showed its molecular mass to be approximately 77 kda. the enzyme was inhibited by specific serine proteinase organic inhibitors, and was active in the pres ...19902087907
antihyperglycemic effect of cucurbita ficifolia fruit extract in streptozotocin-induced diabetic rats.in the present study, the antihyperglycemic effects of cucurbita ficifolia fruit extract were investigated on streptozotocin-induced experimental diabetes in rats. oral administration of the extract (300 and 600 mg/kg body weight, day) for 30 days resulted in a significant reduction in blood glucose, glycosylated haemoglobin, and an increase in plasma insulin and total haemoglobin. the effect was compared with 150 mg/kg b.w. tolbutamide.200616905276
sequence variations in the fad2 gene in seeded pumpkins.seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ...201526782391
[effect of low temperature stress on chilling tolerance and protective system against active oxygen of grafted watermelon].this paper studied the chilling tolerance and the activity of protective system against active oxygen of own-rooted and grafted watermelon seedlings. the results showed that under low temperature stress, the chilling tolerance of the watermelon seedlings grafted on figleaf was higher than that of the seedlings grafted on chaofeng f1, and that of the own-rooted seedlings was the lowest. the decrease of chlorophyll, ascorbate and gsh contents and superoxide dismutase, ascorbate peroxidase and dehy ...200415334965
acute toxicological study of cucurbita ficifolia juice in mice. 200212434522
serine proteinase from cucurbita ficifolia seed; purification, properties, substrate specificity and action on native squash trypsin inhibitor (cmti i).a proteinase was purified from resting seeds of cucurbita ficifolia by ammonium sulfate fractionation and successive chromatography on cm-cellulose, sephacryl s-300 and tsk deae-2sw (hplc) columns. inhibition by dfp and pmsf suggests that the enzyme is a serine proteinase. the apparent molecular mass of this enzyme is ca. 77 kda. the optimum activity for hydrolysis of casein and suc-ala-ala-pro-phe-pna is around ph 10.5. the following peptide bonds in the oxidized insulin b-chain were hydrolysed ...19902291813
[mycotoxins of fusarium spp. in rotten fruits from cucurbita ficifolia].swine acute toxicosis with hemorrhages was recognized after feeding on decayed chayotes (c. ficifolia fruits). in order to elucidate the origin of this pathology, chayotes spoiled by fusaria were analyzed. the mycotoxins (t-2 toxin, fusarenon x) identified in decayed chayotes, the mice alterations and the isolated species (f. equiseli, f. graminearum, f. heterosporum, f. sambucinum var. coeruleum and f. solani), suggest that the effects on swine may be due to trichothecenes produced by fusaria w ...19902103640
can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber?low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ...201627621740
protein inhibitors of trypsin from the seeds of cucurbitaceae plants.seven trypsin inhibitors were isolated from the seeds of cucurbitaceae plants: two from cucumber (cucumis sativus) and red bryony (bryonia diotica) and one from figleaf gourd (cucurbita ficifolia), spaghetti squash (cucurbita pepo var. (vegetable spaghetti) and water melon (citrullus vulgaris). the inhibitors were purified by fractionation with ammonium sulphate, followed by ion-exchange chromatography and affinity chromatography using immobilized trypsin or anhydro-trypsin. the homogeneous inhi ...20073450102
ectopic expression of a foreign aquaporin disrupts the natural expression patterns of endogenous aquaporin genes and alters plant responses to different stress conditions.although the number of reports demonstrating the roles of individual aquaporins in plants under diverse physiological conditions is expanding, the importance of interactions between different aquaporin isoforms and their integrated functions under stress conditions remain unclear. here, we expressed one cucumber aquaporin gene, designated cspip1;1, and one figleaf gourd aquaporin gene, designated cfpip2;1, in arabidopsis thaliana, and investigated the effect of its expression on the natural expr ...200717675323
hypoglycemic activity of some antidiabetic plants.to assess the hypoglycemic activity mechanism of some plants used empirically by the mexican population as antidiabetics, traditional preparations of cucurbita ficifolia, guaiacum coulteri, lepechinia caulescens, and psacalium peltatum, water, tolbutamide, and regular insulin were administered to three groups of rabbits each: 1. healthy rabbits with temporary hyperglycemia induced by the subcutaneous administration of glucose. 2. rabbits with moderate diabetes (fasting glycemia 150-300 mg/dl), i ...19921308798
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