| sequences of the raccoon poxvirus hemagglutinin protein. | primers based on sequences flanking the vaccinia virus (vv) strain ihd hemagglutinin protein (ha) open reading frame (orf) enabled amplification of ha dna segments from the genome of raccoon poxvirus (rcn) and vv strain wr. the amplified segments produced unequal cross-hybridization signal intensities against each other, indicating sequence differences between the ha of rcn (in hindiii-g) and that of vv-wr (in hindiii-a). about 1.5 kb of sequences in the ha region were then determined from clone ... | 1992 | 1529542 |
| coat protein gene-mediated resistance to potato virus y in tobacco: examination of the resistance mechanisms--is the transgenic coat protein required for protection? | we transformed sr1 tobacco with a cdna fragment of the potato virus y strain n (pvyn) 605 genome which contained the 3' end of the nib polymerase gene, the coat protein (cp) coding sequence, and most of the viral 3' untranslated region. complete resistance to several pvy strains was obtained in plants of the r2 generation. the resistance to pvyn and pvyo strains was maintained when the plants were inoculated dually with the potato potyviruses v or a (pvv or pva). only partial resistance to pvyn ... | 1993 | 8324247 |
| analysis of the p1 gene sequences and the 3'-terminal sequences and secondary structures of the single-stranded rna genome of potato virus v. | the immunocapture reverse transcriptase pcr method (ic-rt-pcr) was used to selectively amplify specific genome sequences of an isolate of potato virus v (pvv, genus potyvirus) from a potato plant infected by multiple viruses in the field in finland. the sequences of the 5'- and 3'-non-translated regions (ntr) and the p1-and coat protein (cp)-encoding sequences were determined because they are the most variable genomic regions in potyviruses. the sequences of the new finnish pvv isolate obtained ... | 2001 | 11450952 |
| a mosaic disease of senna hirsuta induced by a potyvirus in nigeria. | a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ... | 2001 | 11719985 |
| the complete nucleotide sequence of pepper mottle virus-florida rna. | the pepper mottle virus-florida (pepmov-fl) rna genome was cloned and sequenced, and shown to consist of 9,717 nucleotides (nt) excluding the poly (a) tail. a single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3068 amino acids. phylogenetic sequence analysis revealed that of 44 full-length viral rna genomes analyzed within the family potyviridae, pepmov-fl was most closely related to pepmov-california (pepmov-ca), potato virus y-h (pvy-h), pvy ... | 2003 | 12536304 |
| genomic sequence of wild potato mosaic virus as compared to the genomes of other potyviruses. | the complete nucleotide sequence of wild potato mosaic virus (wpmv) was determined, showing that it is a distinct member of the genus potyvirus (family potyviridae). the genome consists of 9853 nucleotides and encodes a single polyprotein of 3065 amino acids. the 5'- and 3'-non translated regions (ntr) were 182 and 472 nucleotides, respectively. alignment of the predicted wpmv polyprotein sequence with other members of the genus potyvirus revealed nine putative cleavage sites resulting in ten ma ... | 2003 | 12556999 |
| molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in peru. | peru is a centre of origin and domestication of the potato, pepper and tomato (family solanaceae). many potyviruses (genus potyvirus) that infect these crops were described 20-30 years ago. however, definitive classification of these viruses as distinct species remains unresolved for several reasons, including their close serological relationships, similar symptomatology in test plants and lack of genomic sequence data. using samples collected from peru, we have determined the complete genomic s ... | 2003 | 12917478 |
| noninvasive tests for the prediction of significant hepatic fibrosis in hepatitis c virus carriers with persistently normal alanine aminotransferases. | the diagnostic value of doppler and various noninvasive indices in predicting significant hepatic fibrosis in hepatitis c virus (hcv) carriers with persistently normal alanine aminotransferases (pnalt) is unknown. | 2006 | 17032409 |
| [incidence and altitudinal distribution of 13 virus cultures in solanum tuberosum (solanaceae) from costa rica]. | a survey was conducted in 30 fields located at three different altitudes in cartago, costa rica's main potato producing area. twenty plants were sampled per farm, for a total of 600 samples with 200 samples per altitude. elisa was used with commercial reagents to independently test for pvx, pvy, pvm, pva, pvs, plrv, pmtv, pamv, pvv, pvt, aplv, apmov and trsv. the presence of the following viruses was determined: pvx (77 %), pamv (62 %), plrv (42 %), trsv (42 %), pvt (39 %), pvv (37 %), pmtv (31% ... | 2006 | 18457151 |
| characterization of a new potyvirus infecting pepper crops in ecuador. | sequencing 2,951 nucleotides of the 3' proximal region of the genome of a potyvirus isolate collected from capsicum pubescens (rocoto) pepper in ecuador revealed that this was the first representative of a new species tentatively named ecuadorian rocoto virus (erv). phylogeny reconstruction showed that this isolate clustered with potato virus v (pvv), peru tomato virus and wild potato mosaic virus into a monophyletic group, and was closest to pvv. the isolate was shown to be infectious in tobacc ... | 2008 | 18553171 |
| molecular characterization of potato virus v genomes from europe indicates limited spatiotemporal strain differentiation. | abstract because there were no previous reports on the molecular characterization of potato virus v (pvv, genus potyvirus, family potyviridae), the complete genomic sequence of pvv isolate dv42 was determined. the length of the single-stranded messenger-polarity rna genome was 9,851 nt (nucleotides), followed by a poly(a) tail. the genome contained a 5'-terminal nontranslated region (5'-ntr; 204 nt), a single open reading frame (nucleotides 205-9406; 3,067 amino acids), and a 3'-ntr that was unu ... | 2000 | 18944596 |